MS000035 (gene) Bitter gourd (TR) v1

BLAST of MS000035 vs. NCBI nr
Match: XP_022152059.1 (cytoplasmic tRNA 2-thiolation protein 2 [Momordica charantia])

HSP 1 Score: 920.2 bits (2377), Expect = 7.5e-264
Identity = 473/501 (94.41%), Postives = 474/501 (94.61%), Query Frame = 0

Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60
           MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN
Sbjct: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60

Query: 61  ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 120
           ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV
Sbjct: 61  ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 120

Query: 121 HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS 180
           HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS
Sbjct: 121 HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS 180

Query: 181 KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLIG 240
           KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQK          
Sbjct: 181 KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQK---------- 240

Query: 241 LQSKRGNFSVLLFVLQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDA 300
                           IATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDA
Sbjct: 241 ----------------IATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDA 300

Query: 301 RWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQVQNPSRES 360
           RWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQ +NPSRES
Sbjct: 301 RWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQEENPSRES 360

Query: 361 TIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNI 420
           TIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNI
Sbjct: 361 TIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNI 420

Query: 421 SDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTG 480
           SDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTG
Sbjct: 421 SDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTG 475

Query: 481 NSNSLREQIQEFLLSDDEAES 502
           NSNSLREQIQEFLLSDDEAES
Sbjct: 481 NSNSLREQIQEFLLSDDEAES 475

BLAST of MS000035 vs. NCBI nr
Match: XP_038905927.1 (cytoplasmic tRNA 2-thiolation protein 2 [Benincasa hispida])

HSP 1 Score: 766.9 bits (1979), Expect = 1.1e-217
Identity = 398/500 (79.60%), Postives = 429/500 (85.80%), Query Frame = 0

Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSS CQSNCYRNQ DE T+EQ SPP+++++ AAA+ESC VDTN D  +THLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQVSPPSSRSDTAAAVESCQVDTNDDSSHTHLCFKCKS 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ER+SSSGVGDDGR CS CFRSNLF KFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERISSSGVGDDGRLCSDCFRSNLFGKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAHKNFEAS+DRSLQVFGVGVAYI+ET+A SIPSHE+D  I+TIQSVVS+L PP
Sbjct: 121 VHEMQVKAHKNFEASKDRSLQVFGVGVAYINETAAYSIPSHEIDQTIQTIQSVVSSLCPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLI 240
            K+LHIVQIE+VYSSNL D REKLKKLLDTVNDVTGKEDL+S+LRMLSLQK         
Sbjct: 181 RKDLHIVQIENVYSSNLSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQK--------- 240

Query: 241 GLQSKRGNFSVLLFVLQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVD 300
                            IA ENGYNRLLVGTCTSRIA HVISSTVKGQGYSLPADIQY+D
Sbjct: 241 -----------------IAAENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPADIQYID 300

Query: 301 ARWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQVQNPSRE 360
           ARWE+PV LPL DC+AQEL++LCCVDGLKTVEL K S SGIN LVSSFVALLQV+NPSRE
Sbjct: 301 ARWEIPVVLPLLDCLAQELNLLCCVDGLKTVELTKQSSSGINDLVSSFVALLQVENPSRE 360

Query: 361 STIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLN 420
           STIVRTAGKLTPFNFSRIPDL ES+NVPLATKRRQKRNN KHVNSISSESFCAVCN PLN
Sbjct: 361 STIVRTAGKLTPFNFSRIPDLTESSNVPLATKRRQKRNNFKHVNSISSESFCAVCNGPLN 420

Query: 421 ISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGST 480
            SDVQTLR  E    +++IL  T CSSCQFQILPKDS SQELFLSLLPPSMVARA+HGS 
Sbjct: 421 TSDVQTLRRFEAGQTSSSILGCTCCSSCQFQILPKDSLSQELFLSLLPPSMVARAMHGSF 474

Query: 481 GNSNSLREQIQEFLLSDDEA 500
           GNSNSLREQIQEFLLSDDEA
Sbjct: 481 GNSNSLREQIQEFLLSDDEA 474

BLAST of MS000035 vs. NCBI nr
Match: XP_023539180.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 757.7 bits (1955), Expect = 6.4e-215
Identity = 400/502 (79.68%), Postives = 425/502 (84.66%), Query Frame = 0

Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSSTCQSNCYRNQDDE T+EQ S   NKA+  AA       TNGD G+THLCFKCK+
Sbjct: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ERMSSS  GDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAHKNFEASRDRSLQVFGVGVAYIDETSAC IP HE+D  I+ IQSVVS+L PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQAIQSVVSSLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLI 240
            K+LHIVQIESVYSSNL D REKLKKLLDTV+DVTGKEDL+SHLRMLSLQK         
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQK--------- 240

Query: 241 GLQSKRGNFSVLLFVLQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVD 300
                            IA ENGYNRL++GTCTSRIA HVISSTVKGQGYSLPADIQYVD
Sbjct: 241 -----------------IAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVD 300

Query: 301 ARWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQVQNPSRE 360
           ARWEVPVALPLRDC+AQEL+MLCC DGLKTVEL K SFSGIN LVSSFVALLQ +NPSRE
Sbjct: 301 ARWEVPVALPLRDCLAQELNMLCCADGLKTVELTKTSFSGINDLVSSFVALLQEENPSRE 360

Query: 361 STIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLN 420
           STIVRTAGKLTPFNFSRIPDL+ESN VPLATKRRQKRNN KHVNS+SSE+FCAVCN PLN
Sbjct: 361 STIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLN 420

Query: 421 ISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGST 480
           +SDVQTLRS E   + ++IL+GT CSSCQFQILPKDS SQEL LSLLPPSMVARA+HGS 
Sbjct: 421 MSDVQTLRSFEAG-QVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSP 468

Query: 481 GNSNSLREQIQEFLLSDDEAES 502
            NSNSLREQIQEFLLSDDE ES
Sbjct: 481 SNSNSLREQIQEFLLSDDEVES 468

BLAST of MS000035 vs. NCBI nr
Match: XP_022939604.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita moschata])

HSP 1 Score: 755.4 bits (1949), Expect = 3.2e-214
Identity = 399/502 (79.48%), Postives = 424/502 (84.46%), Query Frame = 0

Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSS CQSNCYRNQDDE T+EQ S   NKA+  AA       TNGD G+THLCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ERMSSS  GDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAHKNFEASRDRSLQVFGVGVAYIDETSAC IP HE+D  I+TIQSVVS+L PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLI 240
            K+LHIVQIESVYSSNL D REKLKKLLDTV+DVTGKEDL+SHLRMLSLQK         
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQK--------- 240

Query: 241 GLQSKRGNFSVLLFVLQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVD 300
                            IA ENGYNRL++GTCTSRIA HVISSTVKGQGYSLPADIQYVD
Sbjct: 241 -----------------IAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVD 300

Query: 301 ARWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQVQNPSRE 360
           ARWEVPVALPLRDC+AQEL+MLCC DGLKT EL K SFSGIN LVSSFVALLQ +NPSRE
Sbjct: 301 ARWEVPVALPLRDCLAQELNMLCCADGLKTEELTKTSFSGINDLVSSFVALLQEENPSRE 360

Query: 361 STIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLN 420
           STIVRTAGKLTPFNFSRIPDL+ESN VPLATKRRQKRNN KHVNS+SSE+FCAVCN PLN
Sbjct: 361 STIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLN 420

Query: 421 ISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGST 480
           +SDVQTLRS E   + ++IL+GT CSSCQFQILPKDS SQEL LSLLPPSMVARA+HGS 
Sbjct: 421 MSDVQTLRSFEAG-QVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSP 468

Query: 481 GNSNSLREQIQEFLLSDDEAES 502
            NSNSLREQIQEFLLSDDE ES
Sbjct: 481 SNSNSLREQIQEFLLSDDEVES 468

BLAST of MS000035 vs. NCBI nr
Match: XP_022971223.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita maxima])

HSP 1 Score: 749.6 bits (1934), Expect = 1.8e-212
Identity = 396/502 (78.88%), Postives = 422/502 (84.06%), Query Frame = 0

Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSS CQSNCYRNQDDE T+EQ S   NK +  AA       T GD G+THLCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAA-------TYGDSGDTHLCFKCKA 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ERMSSS  GDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAHKNFEASRDRSL VFGVGVAYIDETSAC IP HE+D  I+TIQSVVS+L PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLI 240
            K+LHIVQIESVYSSNL D REKLKKLLDTV+DVTGKEDL+SHLRMLSLQK         
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQK--------- 240

Query: 241 GLQSKRGNFSVLLFVLQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVD 300
                            IA ENGYNRL++GTCTSRIA HVISSTVKGQGYSLPADIQYVD
Sbjct: 241 -----------------IAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVD 300

Query: 301 ARWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQVQNPSRE 360
           ARWEVPVALPLRDC+AQEL+MLCC DGLKTVEL K SFSGIN LVSSFVALLQ +NPSRE
Sbjct: 301 ARWEVPVALPLRDCLAQELNMLCCADGLKTVELTKTSFSGINDLVSSFVALLQEENPSRE 360

Query: 361 STIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLN 420
           STIVRTAGKLTPFNFSRIPDL+ESN VPLATKRRQKRNN KHVNS+SSE+FCAVCN PLN
Sbjct: 361 STIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLN 420

Query: 421 ISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGST 480
           +SDVQTLRS E   + ++IL+GT CSSCQFQILPKDS SQEL LSLLPPSMVARA+HGS 
Sbjct: 421 MSDVQTLRSFEAG-QVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSP 468

Query: 481 GNSNSLREQIQEFLLSDDEAES 502
            NSNSLRE+IQEFLLSDDE ES
Sbjct: 481 SNSNSLREKIQEFLLSDDEVES 468

BLAST of MS000035 vs. ExPASy Swiss-Prot
Match: O65628 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 PE=2 SV=3)

HSP 1 Score: 493.0 bits (1268), Expect = 3.9e-138
Identity = 273/499 (54.71%), Postives = 345/499 (69.14%), Query Frame = 0

Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60
           MACNSS C+S CY  + D           +K    A        + G  +  +C KCK N
Sbjct: 1   MACNSSGCESGCYDREKDN---------GSKIVDDAV-------SGGGNHESVCVKCKCN 60

Query: 61  ERMS-SSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
             M+   G  DDGRFC+ CFR+N+F KFRLAVTS+AMI+PSD VLVAFSGG  SRV+LQF
Sbjct: 61  APMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHELQ+KA KN+EASRDRSL VFGVGVA++DET+A    S EM  AIE ++  VS L PP
Sbjct: 121 VHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLI 240
           +K+LH+V +ES++ S+  D R++L KLLD+V D TGKEDLL HL+MLSLQKV        
Sbjct: 181 AKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKV-------- 240

Query: 241 GLQSKRGNFSVLLFVLQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVD 300
                             A ENGYNRL++G+CTSRIA HV+++TVKG+GYSL ADIQ+VD
Sbjct: 241 ------------------AAENGYNRLVLGSCTSRIASHVLTATVKGRGYSLSADIQHVD 300

Query: 301 ARWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQVQNPSRE 360
           ARW+VP+ LPLRDC+  E+  LC +DGLKTVELA  S  GIN LVSSFVALLQ +NPSRE
Sbjct: 301 ARWKVPIVLPLRDCVRLEITRLCLLDGLKTVELACRSQCGINDLVSSFVALLQEENPSRE 360

Query: 361 STIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLN 420
            TIVRTA KLTPF F++IP+ D+S NVP+AT+RR KR N+K+  S+++E+FC +CN PLN
Sbjct: 361 CTIVRTAAKLTPFYFNKIPETDDS-NVPMATQRRLKRFNLKYDGSMTTEAFCPICNGPLN 420

Query: 421 ISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGST 480
            SD   L + E   E +++L    CSSC+FQILP+D +S E F S LP  M+++  H   
Sbjct: 421 RSDSSELDTFEEGQE-SDVLYAACCSSCRFQILPQDGSSLEQFSSFLPDHMISQVKHQKV 455

Query: 481 GNSNSLREQIQEFLLSDDE 499
            +   LRE+I++ LL DDE
Sbjct: 481 DSQAYLREKIKDCLLLDDE 455

BLAST of MS000035 vs. ExPASy Swiss-Prot
Match: A2ZM73 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=CTU2 PE=3 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 6.7e-98
Identity = 206/472 (43.64%), Postives = 289/472 (61.23%), Query Frame = 0

Query: 29  ANKAEAAAAMESCPVDTNGDGNTHLCFKC-KSNERMSSSGVGDDGRFCSGCFRSNLFAKF 88
           ++ A AAA  ++        G   L  +C K     ++  V      C  CFR+NL  KF
Sbjct: 19  SSSASAAAVEDAAAAAAEKVGRLSLSRECGKCGGGAAAVAVAGGLGLCGECFRANLLGKF 78

Query: 89  RLAVTSNAMISPSDKVLVAFSGGPCSRVALQFVHELQVKAHKNFEASRDRSLQVFGVGVA 148
           +LAVTSNAM+ P+D VL+AFSGGP SRVALQF+HE++ KA ++++ S  ++L VFGVGVA
Sbjct: 79  KLAVTSNAMVRPTDSVLLAFSGGPASRVALQFIHEMRCKAIESWDVSNSQALPVFGVGVA 138

Query: 149 YIDETSACSIPSHEMDSAIETIQSVVSNLRPPSKELHIVQIESVYSSNLGDRREKLKKLL 208
           ++DE+  CS P  E++ AIE I+S+VS+L    K +HIV++E V+S+   D   +L++ +
Sbjct: 139 FVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGVKAMHIVRLEDVFSTESEDGERRLREAV 198

Query: 209 DTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLIGLQSKRGNFSVLLFVLQIATENGYNRLL 268
           D + D TG+ED L  LRMLSLQK                          IA ENGY +++
Sbjct: 199 DMIGDDTGREDFLRCLRMLSLQK--------------------------IAMENGYAKIM 258

Query: 269 VGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVDGL 328
           +G+C S IA HV+S+TVKGQGYSLPAD+QYVD RWE+PV LPLRDC+AQEL +LC +D L
Sbjct: 259 LGSCASAIACHVLSATVKGQGYSLPADVQYVDTRWEIPVVLPLRDCLAQELTLLCELDSL 318

Query: 329 KTVELAKNSFSGINGLVSSFVALLQVQNPSRESTIVRTAGKLTPFNFSRIPDLDESNNVP 388
           KT +      +GIN LV+SF+  L+ +NPSRE TIVRTA KL PF+F++       + +P
Sbjct: 319 KTQQHLDRPSNGINSLVASFIKRLREENPSREHTIVRTAQKLKPFSFNKFSADGYHDFLP 378

Query: 389 LATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCE-TSPETANILSGTWCSS 448
             ++ R K   V    S  SE  C +C SP + S++Q L S +  + +  ++ +   C S
Sbjct: 379 --SRLRPKFQKVDSDESTFSEILCLMCGSPFSESELQNLESTKHKAQKKIDLYTAHCCQS 438

Query: 449 CQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDE 499
           C FQILP      E F SLLP     +    S  +S  LR+QI+E+LL +++
Sbjct: 439 CYFQILPAGENLNEHFFSLLPKLWTGKMDTISDSHS-LLRDQIEEYLLEEND 461

BLAST of MS000035 vs. ExPASy Swiss-Prot
Match: Q2QMW0 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CTU2 PE=2 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 7.4e-97
Identity = 204/472 (43.22%), Postives = 287/472 (60.81%), Query Frame = 0

Query: 29  ANKAEAAAAMESCPVDTNGDGNTHLCFKC-KSNERMSSSGVGDDGRFCSGCFRSNLFAKF 88
           ++ A AAA  ++        G   L  +C K     ++  V      C  CFR+NL  KF
Sbjct: 19  SSSASAAAVEDAAAAAAEKVGRLSLSRECGKCGGGAAAVAVAGGLGLCGECFRANLLGKF 78

Query: 89  RLAVTSNAMISPSDKVLVAFSGGPCSRVALQFVHELQVKAHKNFEASRDRSLQVFGVGVA 148
           +LAVTSNAM+ P+D VL+AFSGGP SRVALQF+HE++ KA ++++ S  ++L VFGVGVA
Sbjct: 79  KLAVTSNAMVRPTDSVLLAFSGGPASRVALQFIHEMRCKAIESWDVSNSQALPVFGVGVA 138

Query: 149 YIDETSACSIPSHEMDSAIETIQSVVSNLRPPSKELHIVQIESVYSSNLGDRREKLKKLL 208
           ++DE+  CS P  E++ AIE I+S+VS+L    K +HI ++E V+S+   D   +L++ +
Sbjct: 139 FVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGVKAMHIARLEDVFSTESEDGERRLREAV 198

Query: 209 DTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLIGLQSKRGNFSVLLFVLQIATENGYNRLL 268
           D + D TG+ED L  LRMLSLQK                          IA ENGY +++
Sbjct: 199 DMIGDDTGREDFLRCLRMLSLQK--------------------------IAMENGYAKIM 258

Query: 269 VGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVDGL 328
           +G+C S IA HV+S+TVKGQGYSLPAD+QYVD RWE+PV LPLRDC+AQEL +LC +D L
Sbjct: 259 LGSCASAIACHVLSATVKGQGYSLPADVQYVDTRWEIPVVLPLRDCLAQELTLLCELDSL 318

Query: 329 KTVELAKNSFSGINGLVSSFVALLQVQNPSRESTIVRTAGKLTPFNFSRIPDLDESNNVP 388
           KT +      +GIN LV+SF+  L+ +NPSRE TIVRTA KL PF+F++       + +P
Sbjct: 319 KTQQHLDRPSNGINSLVASFIKRLREENPSREHTIVRTAQKLKPFSFNKFSADGYHDFLP 378

Query: 389 LATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCE-TSPETANILSGTWCSS 448
             ++ R K        S  SE  C +C SP + S++Q L S +  + +  ++ +   C S
Sbjct: 379 --SRLRPKFQKFDSDESTFSEILCLMCGSPFSESELQNLESTKHKAQKKIDLYTAHCCQS 438

Query: 449 CQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDE 499
           C FQILP      E F SLLP     +    S  +S  LR+QI+E+LL +++
Sbjct: 439 CYFQILPAGENLNEHFFSLLPKLWTGKMDTISDSHS-LLRDQIEEYLLEEND 461

BLAST of MS000035 vs. ExPASy Swiss-Prot
Match: Q08B12 (Cytoplasmic tRNA 2-thiolation protein 2-A OS=Xenopus laevis OX=8355 GN=ctu2-a PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 2.9e-24
Identity = 132/515 (25.63%), Postives = 214/515 (41.55%), Query Frame = 0

Query: 53  LCFKCKSNERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPC 112
           +C KCK +        GD   FC  CF+     KFR  +  N +I P +KVL+A+SGGP 
Sbjct: 24  ICMKCKESSAALLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEKVLLAYSGGPS 83

Query: 113 SRVALQFVHELQVKAHKNFEASRDRSLQV-FGVGVAYIDETSACSIPSHEMDSAIETIQS 172
           S   ++ V E           SRD   ++ F  G+ +IDE +AC +   E    +  I S
Sbjct: 84  SSAMVRQVQE---------GLSRDAPKKLRFVPGILFIDEGTACGMSWEERQQILSEICS 143

Query: 173 VVSNLRPPSKELHIVQIESVYS----------------------------SNLGDR--RE 232
           V+   + P    HIV +E V+S                               GD    E
Sbjct: 144 VLQQTKIP---FHIVSLEQVFSLPGSVLQRGAPEQRPNYKEEVDRFLVQEREQGDAGCSE 203

Query: 233 KLKKLLDTVNDVTGKEDLL-----SHLRMLSLQKVCPSIYSLIGLQSKRGNFSVLL--FV 292
            L++L  T +D  G  D +     SH   +  QK+     S   L +K+     L    +
Sbjct: 204 MLERLEVTDSDSPGSSDKMYQSTCSHPPDMHTQKLKQLFASAKTLTAKQQLLHTLRSHLI 263

Query: 293 LQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARW-EVPVALPLRDC 352
           L IA   GY++++ G   +R++  ++S+   G+G  LP D  + D+R+ +V +  P+R+ 
Sbjct: 264 LHIARTCGYSKVMTGESCTRLSIRLLSNVSLGRGAFLPLDTGFCDSRYGDVDIIRPMREY 323

Query: 353 IAQELHMLCCVDGLKTV-----ELAKNSFSGINGLVSSFVALLQVQNPSRESTIVRTAGK 412
            ++E+        +  +     +   +  S I  L   FV  LQ   PS  ST+ RT+ K
Sbjct: 324 SSKEIAYYNRFFNVSPIFIPALDTKASENSSIQHLTEVFVNRLQADFPSTVSTLYRTSEK 383

Query: 413 LTPFNFSRIPDLDESNNV---------PLATKRRQ----KRNNVKH---------VNSIS 472
           L   N S+I D D+             PL T+  +        + H          N ++
Sbjct: 384 L---NVSKI-DADQETCAKDRCLLCLSPLDTQAGKASAFSATQLSHHLSQKIPMKSNDLA 443

Query: 473 SESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLL 502
           + S  + C       +     +C++            C SC+  +  KD  S    L +L
Sbjct: 444 NNSDKSCCQGGQGCKEAGYGDTCQSRALQTPSFVHMLCYSCRLTV--KDMQS----LDVL 503

BLAST of MS000035 vs. ExPASy Swiss-Prot
Match: Q32NV1 (Cytoplasmic tRNA 2-thiolation protein 2-B OS=Xenopus laevis OX=8355 GN=ctu2-b PE=2 SV=2)

HSP 1 Score: 107.5 bits (267), Expect = 4.6e-22
Identity = 130/515 (25.24%), Postives = 212/515 (41.17%), Query Frame = 0

Query: 53  LCFKCKSNERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPC 112
           +C KCK +        GD   FC  CF+     KFR  +  N +I P +KVL+A+SGGP 
Sbjct: 24  ICMKCKESSAALLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEKVLLAYSGGPS 83

Query: 113 SRVALQFVHELQVKAHKNFEASRDRSLQV-FGVGVAYIDETSACSIPSHEMDSAIETIQS 172
           S   ++ V E           SRD   ++ F  G+ +IDE +AC +   E    +  I S
Sbjct: 84  SSAMVRQVQE---------GLSRDAPKKLRFVPGILFIDEGTACGMSWEERQQILSEICS 143

Query: 173 VVSNLRPPSKELHIVQIESVYS----------------------------SNLGDR--RE 232
           V+   + P    HIV +E V+S                               GD    E
Sbjct: 144 VLQQTKIP---FHIVSLEQVFSLPGSVLQRGAPEQRPNYKEEVDRFLVQEREQGDAGCSE 203

Query: 233 KLKKLLDTVNDVTGKEDLL-----SHLRMLSLQKVCPSIYSLIGLQSKRGNFSVLL--FV 292
            L++L  T +D  G  D +     S    +  QK+     S   L +K+     L    +
Sbjct: 204 MLERLEVTDSDSPGSSDKMYQSTCSRPPDMHTQKLKQLFASAKTLTAKQQLLHTLRSHLI 263

Query: 293 LQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARW-EVPVALPLRDC 352
           L IA   GY++++ G   +R++  ++S+   G+G  LP D  + D+R+ +V +  P+R+ 
Sbjct: 264 LHIARTCGYSKVMTGESCTRLSIRLLSNVSLGRGAFLPLDTGFCDSRYGDVDIIRPMREY 323

Query: 353 IAQEL-----HMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQVQNPSRESTIVRTAGK 412
            ++E+             +  ++   +  S I  L   FV  LQ   PS  ST+ RT+ K
Sbjct: 324 SSKEIAYYNRFFNVLPIFIPALDTKASENSSIQHLTEVFVNRLQADFPSTVSTLYRTSEK 383

Query: 413 LTPFNFSRIPDLDESNNV---------PLATKRRQ----KRNNVKH---------VNSIS 472
           L   N S I D D+             PL T+  +        + H          N ++
Sbjct: 384 L---NVS-IIDADQETCAKDRCLLCLSPLDTQAGKASAFSATQLSHHLSQKIPMKSNDLA 443

Query: 473 SESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLL 502
           + S  + C       +     +C++            C SC+  +  KD  S    L +L
Sbjct: 444 NNSDKSCCQGGQGCKEAGYGDTCQSRALQTPSFVHMLCYSCRLTV--KDMQS----LDVL 503

BLAST of MS000035 vs. ExPASy TrEMBL
Match: A0A6J1DEW9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Momordica charantia OX=3673 GN=LOC111019863 PE=3 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 3.6e-264
Identity = 473/501 (94.41%), Postives = 474/501 (94.61%), Query Frame = 0

Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60
           MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN
Sbjct: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60

Query: 61  ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 120
           ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV
Sbjct: 61  ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 120

Query: 121 HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS 180
           HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS
Sbjct: 121 HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS 180

Query: 181 KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLIG 240
           KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQK          
Sbjct: 181 KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQK---------- 240

Query: 241 LQSKRGNFSVLLFVLQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDA 300
                           IATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDA
Sbjct: 241 ----------------IATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDA 300

Query: 301 RWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQVQNPSRES 360
           RWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQ +NPSRES
Sbjct: 301 RWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQEENPSRES 360

Query: 361 TIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNI 420
           TIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNI
Sbjct: 361 TIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNI 420

Query: 421 SDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTG 480
           SDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTG
Sbjct: 421 SDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTG 475

Query: 481 NSNSLREQIQEFLLSDDEAES 502
           NSNSLREQIQEFLLSDDEAES
Sbjct: 481 NSNSLREQIQEFLLSDDEAES 475

BLAST of MS000035 vs. ExPASy TrEMBL
Match: A0A6J1FHP7 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111445447 PE=3 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.5e-214
Identity = 399/502 (79.48%), Postives = 424/502 (84.46%), Query Frame = 0

Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSS CQSNCYRNQDDE T+EQ S   NKA+  AA       TNGD G+THLCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ERMSSS  GDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAHKNFEASRDRSLQVFGVGVAYIDETSAC IP HE+D  I+TIQSVVS+L PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLI 240
            K+LHIVQIESVYSSNL D REKLKKLLDTV+DVTGKEDL+SHLRMLSLQK         
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQK--------- 240

Query: 241 GLQSKRGNFSVLLFVLQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVD 300
                            IA ENGYNRL++GTCTSRIA HVISSTVKGQGYSLPADIQYVD
Sbjct: 241 -----------------IAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVD 300

Query: 301 ARWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQVQNPSRE 360
           ARWEVPVALPLRDC+AQEL+MLCC DGLKT EL K SFSGIN LVSSFVALLQ +NPSRE
Sbjct: 301 ARWEVPVALPLRDCLAQELNMLCCADGLKTEELTKTSFSGINDLVSSFVALLQEENPSRE 360

Query: 361 STIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLN 420
           STIVRTAGKLTPFNFSRIPDL+ESN VPLATKRRQKRNN KHVNS+SSE+FCAVCN PLN
Sbjct: 361 STIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLN 420

Query: 421 ISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGST 480
           +SDVQTLRS E   + ++IL+GT CSSCQFQILPKDS SQEL LSLLPPSMVARA+HGS 
Sbjct: 421 MSDVQTLRSFEAG-QVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSP 468

Query: 481 GNSNSLREQIQEFLLSDDEAES 502
            NSNSLREQIQEFLLSDDE ES
Sbjct: 481 SNSNSLREQIQEFLLSDDEVES 468

BLAST of MS000035 vs. ExPASy TrEMBL
Match: A0A6J1I684 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111470013 PE=3 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 8.5e-213
Identity = 396/502 (78.88%), Postives = 422/502 (84.06%), Query Frame = 0

Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSS CQSNCYRNQDDE T+EQ S   NK +  AA       T GD G+THLCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAA-------TYGDSGDTHLCFKCKA 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ERMSSS  GDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAHKNFEASRDRSL VFGVGVAYIDETSAC IP HE+D  I+TIQSVVS+L PP
Sbjct: 121 VHEMQVKAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLI 240
            K+LHIVQIESVYSSNL D REKLKKLLDTV+DVTGKEDL+SHLRMLSLQK         
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQK--------- 240

Query: 241 GLQSKRGNFSVLLFVLQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVD 300
                            IA ENGYNRL++GTCTSRIA HVISSTVKGQGYSLPADIQYVD
Sbjct: 241 -----------------IAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVD 300

Query: 301 ARWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQVQNPSRE 360
           ARWEVPVALPLRDC+AQEL+MLCC DGLKTVEL K SFSGIN LVSSFVALLQ +NPSRE
Sbjct: 301 ARWEVPVALPLRDCLAQELNMLCCADGLKTVELTKTSFSGINDLVSSFVALLQEENPSRE 360

Query: 361 STIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLN 420
           STIVRTAGKLTPFNFSRIPDL+ESN VPLATKRRQKRNN KHVNS+SSE+FCAVCN PLN
Sbjct: 361 STIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLN 420

Query: 421 ISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGST 480
           +SDVQTLRS E   + ++IL+GT CSSCQFQILPKDS SQEL LSLLPPSMVARA+HGS 
Sbjct: 421 MSDVQTLRSFEAG-QVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSP 468

Query: 481 GNSNSLREQIQEFLLSDDEAES 502
            NSNSLRE+IQEFLLSDDE ES
Sbjct: 481 SNSNSLREKIQEFLLSDDEVES 468

BLAST of MS000035 vs. ExPASy TrEMBL
Match: A0A0A0LC57 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis sativus OX=3659 GN=Csa_3G816060 PE=3 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 1.1e-209
Identity = 388/502 (77.29%), Postives = 419/502 (83.47%), Query Frame = 0

Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60
           MACNSS CQSNCYRNQ DE T+EQ SPP+++ +  AAME+  V T  D  NT+LCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60

Query: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
            ERMS+SGVGDDGRFCS CFRSNLFAKFR AVTSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHE+QVKAH NFEASRDRSLQVFGVGVAYI+ET+ACSIPS E+D  ++TIQSVVS+L  P
Sbjct: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLI 240
            K LHIVQIESVYSSNL D REKLKKLLD VNDVTGKEDL+S+LRMLSLQK         
Sbjct: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQK--------- 240

Query: 241 GLQSKRGNFSVLLFVLQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVD 300
                            IA ENGYNRLLVGTCTSRIA HVISSTVKGQGYSLP D+QY+D
Sbjct: 241 -----------------IAAENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPGDVQYID 300

Query: 301 ARWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQVQNPSRE 360
           ARWEVPV LPL DC+AQEL+MLCCVDGLKTVEL K S SGIN LVSSFVALLQV+NPSRE
Sbjct: 301 ARWEVPVVLPLLDCLAQELNMLCCVDGLKTVELTKKSSSGINDLVSSFVALLQVENPSRE 360

Query: 361 STIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLN 420
           STIVRTAGKLTPFNFSRIPDL+ESNNVPLATKRRQ+RNN KHVNS+SSESFCAVCN PLN
Sbjct: 361 STIVRTAGKLTPFNFSRIPDLNESNNVPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLN 420

Query: 421 ISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGST 480
            SDV T  +        + + G  CSSCQFQILPKDS+SQELFLSLLPPSMVARA HGS 
Sbjct: 421 TSDVLTRHA-------GSSILGACCSSCQFQILPKDSSSQELFLSLLPPSMVARATHGSL 469

Query: 481 GNSNSLREQIQEFLLSDDEAES 502
           GNSNSLR++IQEFLLSDDEAES
Sbjct: 481 GNSNSLRKKIQEFLLSDDEAES 469

BLAST of MS000035 vs. ExPASy TrEMBL
Match: A0A1S3B7H9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 PE=3 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 3.5e-206
Identity = 382/501 (76.25%), Postives = 416/501 (83.03%), Query Frame = 0

Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60
           MACNSS CQSNCYRNQ DE T+EQ SPP+++   AA  ES  +  + + NT+LCFKCKS 
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGES-RITNDDNSNTNLCFKCKSK 60

Query: 61  ERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 120
           ERMS+SGVGDDGR CS CFRSNLFAKFR AVTSNAMISPSDKVLVAFSGGPCSRVALQFV
Sbjct: 61  ERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 120

Query: 121 HELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPPS 180
           HE+QVKAHKNFEASRDRSLQVFGVGVAYI+ET+A SIPS E+D  I+TIQSVVS+L  P 
Sbjct: 121 HEIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQPR 180

Query: 181 KELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLIG 240
           K+LHIV IE+VYSSNL D REKLKKLLD V+DVTGKEDL+S+LRMLSLQK          
Sbjct: 181 KDLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQK---------- 240

Query: 241 LQSKRGNFSVLLFVLQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDA 300
                           IA ENGYNRLLVGTCTSRIA HVISSTVKGQGYSLPAD+QY+DA
Sbjct: 241 ----------------IAAENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDA 300

Query: 301 RWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQVQNPSRES 360
           RWEVPV LPL DC+AQEL+MLCC+DGLKTVEL K S SGIN LVSSFVALLQV+NPSRES
Sbjct: 301 RWEVPVVLPLLDCLAQELNMLCCIDGLKTVELTKKSSSGINDLVSSFVALLQVENPSRES 360

Query: 361 TIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNI 420
           TIVRTAGKLTPFNFSRIPDL+ESNNVPLATKRRQKRNN KHVNS+SSESFCAVCN PLN 
Sbjct: 361 TIVRTAGKLTPFNFSRIPDLNESNNVPLATKRRQKRNNFKHVNSLSSESFCAVCNGPLNT 420

Query: 421 SDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTG 480
           SDVQT  +       ++ + G  CSSCQFQILPKDS S ELFLSLLPPSMVARA HGS G
Sbjct: 421 SDVQTRHA-------SSSILGACCSSCQFQILPKDSLSHELFLSLLPPSMVARATHGSLG 467

Query: 481 NSNSLREQIQEFLLSDDEAES 502
           NSNSLR +IQEFLLSDD+AES
Sbjct: 481 NSNSLRNKIQEFLLSDDDAES 467

BLAST of MS000035 vs. TAIR 10
Match: AT4G35910.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 493.0 bits (1268), Expect = 2.8e-139
Identity = 273/499 (54.71%), Postives = 345/499 (69.14%), Query Frame = 0

Query: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGDGNTHLCFKCKSN 60
           MACNSS C+S CY  + D           +K    A        + G  +  +C KCK N
Sbjct: 1   MACNSSGCESGCYDREKDN---------GSKIVDDAV-------SGGGNHESVCVKCKCN 60

Query: 61  ERMS-SSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
             M+   G  DDGRFC+ CFR+N+F KFRLAVTS+AMI+PSD VLVAFSGG  SRV+LQF
Sbjct: 61  APMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQF 120

Query: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180
           VHELQ+KA KN+EASRDRSL VFGVGVA++DET+A    S EM  AIE ++  VS L PP
Sbjct: 121 VHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPP 180

Query: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKVCPSIYSLI 240
           +K+LH+V +ES++ S+  D R++L KLLD+V D TGKEDLL HL+MLSLQKV        
Sbjct: 181 AKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKV-------- 240

Query: 241 GLQSKRGNFSVLLFVLQIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVD 300
                             A ENGYNRL++G+CTSRIA HV+++TVKG+GYSL ADIQ+VD
Sbjct: 241 ------------------AAENGYNRLVLGSCTSRIASHVLTATVKGRGYSLSADIQHVD 300

Query: 301 ARWEVPVALPLRDCIAQELHMLCCVDGLKTVELAKNSFSGINGLVSSFVALLQVQNPSRE 360
           ARW+VP+ LPLRDC+  E+  LC +DGLKTVELA  S  GIN LVSSFVALLQ +NPSRE
Sbjct: 301 ARWKVPIVLPLRDCVRLEITRLCLLDGLKTVELACRSQCGINDLVSSFVALLQEENPSRE 360

Query: 361 STIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAVCNSPLN 420
            TIVRTA KLTPF F++IP+ D+S NVP+AT+RR KR N+K+  S+++E+FC +CN PLN
Sbjct: 361 CTIVRTAAKLTPFYFNKIPETDDS-NVPMATQRRLKRFNLKYDGSMTTEAFCPICNGPLN 420

Query: 421 ISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVARAIHGST 480
            SD   L + E   E +++L    CSSC+FQILP+D +S E F S LP  M+++  H   
Sbjct: 421 RSDSSELDTFEEGQE-SDVLYAACCSSCRFQILPQDGSSLEQFSSFLPDHMISQVKHQKV 455

Query: 481 GNSNSLREQIQEFLLSDDE 499
            +   LRE+I++ LL DDE
Sbjct: 481 DSQAYLREKIKDCLLLDDE 455