MS000012 (gene) Bitter gourd (TR) v1

BLAST of MS000012 vs. NCBI nr
Match: XP_022152042.1 (NF-kappa-B-activating protein [Momordica charantia])

HSP 1 Score: 937.6 bits (2422), Expect = 4.7e-269
Identity = 519/521 (99.62%), Postives = 520/521 (99.81%), Query Frame = 0

Query: 1   MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDYRRRSPSYESYDRYNHRRRSDSPR 60
           MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDYRRRSPSYESYDRYNHRRRSDSPR
Sbjct: 1   MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDYRRRSPSYESYDRYNHRRRSDSPR 60

Query: 61  YHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKRQ 120
           YHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKRQ
Sbjct: 61  YHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKRQ 120

Query: 121 KLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCGDDGGYRSGSGEIKQHEEKYESDK 180
           KLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCGDDGGYRSGSGEIKQHEEKYESDK
Sbjct: 121 KLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCGDDGGYRSGSGEIKQHEEKYESDK 180

Query: 181 TKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTESGSESESESESEEESRRRRK 240
           TKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTESGS SESESESEEESRRRRK
Sbjct: 181 TKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTESGSASESESESEEESRRRRK 240

Query: 241 KSKNRRRNRKHKNSSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRTRSSRKR 300
           KSKNRRRNRKHKNSSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRTRSSRKR
Sbjct: 241 KSKNRRRNRKHKNSSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRTRSSRKR 300

Query: 301 RYSDSDTDEGEESEGRKLRKSKTSSKSRSKRKRQSETESKSPASEKNSGSEDVDANSKLK 360
           RYSDSDTDEGEESEGRKLRKSKTSSKSRSKRKRQSETESKSPASE+NSGSEDVDANSKLK
Sbjct: 301 RYSDSDTDEGEESEGRKLRKSKTSSKSRSKRKRQSETESKSPASEENSGSEDVDANSKLK 360

Query: 361 VDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGALRPGEGDAI 420
           VDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGALRPGEGDAI
Sbjct: 361 VDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGALRPGEGDAI 420

Query: 421 AQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQVYSAEDKRA 480
           AQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQVYSAEDKRA
Sbjct: 421 AQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQVYSAEDKRA 480

Query: 481 LAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 522
           LAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA
Sbjct: 481 LAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 521

BLAST of MS000012 vs. NCBI nr
Match: KAG6580871.1 (NKAP family protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 773.1 bits (1995), Expect = 1.5e-219
Identity = 447/530 (84.34%), Postives = 481/530 (90.75%), Query Frame = 0

Query: 1   MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDY-RRRSPSYESYDRYNHRRRSDSP 60
           MGRLGSTVEIPERRHRSDRE+ +HRY+PDSDASGGDY RRRSPSYESYDRY+HRRRS SP
Sbjct: 1   MGRLGSTVEIPERRHRSDRENGSHRYSPDSDASGGDYRRRRSPSYESYDRYSHRRRSVSP 60

Query: 61  RYHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKR 120
            Y D+RRSPR+DGD+NGLPKRFGRAGGRAYLDRNGR SEES+SDEELKGLNYE+YRR+KR
Sbjct: 61  GYQDIRRSPRADGDQNGLPKRFGRAGGRAYLDRNGRASEESDSDEELKGLNYEDYRRMKR 120

Query: 121 QKLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCG-DDGGYRSGSGEIKQHEEKYES 180
           QKLRKTLKHCIWRVTPSPPRN N+D+E+K DEIL+K G DDGG  SG  E KQHE+KYES
Sbjct: 121 QKLRKTLKHCIWRVTPSPPRNGNEDYEDKDDEILEKYGNDDGGDISGLSEKKQHEKKYES 180

Query: 181 DKTKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTESGS--ESESESESEEESR 240
           DK KNSDS SDSELSDTK K+++LKSSGSR RSRKSS SD+ESGS  ESESESESEEESR
Sbjct: 181 DKAKNSDSDSDSELSDTKLKRRKLKSSGSRRRSRKSSLSDSESGSDTESESESESEEESR 240

Query: 241 RRRKKSKNRRRNRKHKN--SSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRT 300
           +RRKKS N RRNRKHK+  SS KKKKS YSDTED EESETDDSD SD V+S+KR R KR+
Sbjct: 241 KRRKKSTN-RRNRKHKSIRSSKKKKKSRYSDTEDSEESETDDSDASDHVKSRKRSRRKRS 300

Query: 301 RSSRKRRYSDSDTDEGEESEGRKLRK---SKTSSKSRSKRKRQSETESKSPASEKNSGSE 360
           ++ RKRRYSDS  D+ E SEG KLRK   S TS+KSRSK+ RQSETESK  +SE+NSGSE
Sbjct: 301 KNGRKRRYSDS--DDSENSEGEKLRKRKSSSTSTKSRSKKNRQSETESKGSSSEENSGSE 360

Query: 361 DVDANSKLKVDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGA 420
           DVDA SKLK+DG+KM E+NAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGA
Sbjct: 361 DVDAKSKLKIDGDKMAEINAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGA 420

Query: 421 LRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQ 480
           LRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQ FETLGYVMSGSRHQRMNAIRIRKENQ
Sbjct: 421 LRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQNFETLGYVMSGSRHQRMNAIRIRKENQ 480

Query: 481 VYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 522
           VYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA
Sbjct: 481 VYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 527

BLAST of MS000012 vs. NCBI nr
Match: KAG7017627.1 (NKAP family protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 769.6 bits (1986), Expect = 1.7e-218
Identity = 445/530 (83.96%), Postives = 480/530 (90.57%), Query Frame = 0

Query: 1   MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDY-RRRSPSYESYDRYNHRRRSDSP 60
           MGRLGSTVEIPERRHRSDRE+  HRY+PDSDASGGDY RRRSPSYESYDRY+HRRRS SP
Sbjct: 1   MGRLGSTVEIPERRHRSDRENGRHRYSPDSDASGGDYRRRRSPSYESYDRYSHRRRSVSP 60

Query: 61  RYHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKR 120
            Y D+RRSPR+DGD+NGLPKRFGRAGGRAYLDRNGR SEES+SDEELKGLNYE+YRR+KR
Sbjct: 61  GYQDIRRSPRADGDQNGLPKRFGRAGGRAYLDRNGRASEESDSDEELKGLNYEDYRRMKR 120

Query: 121 QKLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCG-DDGGYRSGSGEIKQHEEKYES 180
           QKLRKTLKHCIWRVTPSPPRN N+D+E+K DEIL+K G DDGG  SG  E KQHE+KYES
Sbjct: 121 QKLRKTLKHCIWRVTPSPPRNGNEDYEDKDDEILEKYGNDDGGDISGLSEKKQHEKKYES 180

Query: 181 DKTKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTESGS--ESESESESEEESR 240
           DK KNSDS SDSELSDTK K+++LKSSGSR RSRKSS SD+E+GS  ESESESESEEESR
Sbjct: 181 DKAKNSDSDSDSELSDTKLKRRKLKSSGSRRRSRKSSLSDSETGSDTESESESESEEESR 240

Query: 241 RRRKKSKNRRRNRKHKN--SSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRT 300
           +RRKKS N RRNRKHK+  SS KKKKS YSDTED EES+TDDSD SD V+S+KR R KR+
Sbjct: 241 KRRKKSTN-RRNRKHKSIRSSKKKKKSRYSDTEDSEESKTDDSDASDHVKSRKRSRRKRS 300

Query: 301 RSSRKRRYSDSDTDEGEESEGRKLRK---SKTSSKSRSKRKRQSETESKSPASEKNSGSE 360
           ++ RKRRYSDS  D+ E SEG KLRK   S TS+KSRSK+ RQSETESK  +SE+NSGSE
Sbjct: 301 KNGRKRRYSDS--DDSENSEGEKLRKRKSSSTSTKSRSKKNRQSETESKGSSSEENSGSE 360

Query: 361 DVDANSKLKVDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGA 420
           DVDA SKLK+DG+KM E+NAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGA
Sbjct: 361 DVDAKSKLKIDGDKMAEINAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGA 420

Query: 421 LRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQ 480
           LRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQ FETLGYVMSGSRHQRMNAIRIRKENQ
Sbjct: 421 LRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQNFETLGYVMSGSRHQRMNAIRIRKENQ 480

Query: 481 VYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 522
           VYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA
Sbjct: 481 VYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 527

BLAST of MS000012 vs. NCBI nr
Match: XP_023522495.1 (NF-kappa-B-activating protein-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023522496.1 NF-kappa-B-activating protein-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023522497.1 NF-kappa-B-activating protein-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023522498.1 NF-kappa-B-activating protein-like isoform X3 [Cucurbita pepo subsp. pepo] >XP_023527311.1 NF-kappa-B-activating protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 766.1 bits (1977), Expect = 1.9e-217
Identity = 446/529 (84.31%), Postives = 477/529 (90.17%), Query Frame = 0

Query: 1   MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDY-RRRSPSYESYDRYNHRRRSDSP 60
           MGRLGSTVEIPERRHRSDRE+ +HRY+PDSDASGGDY RRRSPSYESYDRY+HRRRS SP
Sbjct: 1   MGRLGSTVEIPERRHRSDRENGSHRYSPDSDASGGDYRRRRSPSYESYDRYSHRRRSVSP 60

Query: 61  RYHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKR 120
            Y D RRSPR+DGD+NGLPKRFGRAGGRAYLDRNGR S+ES+SDEELKGLNYE+YRRLKR
Sbjct: 61  GYQDNRRSPRADGDQNGLPKRFGRAGGRAYLDRNGRASDESDSDEELKGLNYEDYRRLKR 120

Query: 121 QKLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCG-DDGGYRSGSGEIKQHEEKYES 180
           QKLRKTLKHCIWRVTPSPPRN N+D+E+K DEIL+K G DD G  SG  E KQHE+KY S
Sbjct: 121 QKLRKTLKHCIWRVTPSPPRNGNEDYEDKDDEILEKYGNDDDGDISGLSEKKQHEKKYAS 180

Query: 181 DKTKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTESGS--ESESESESEEESR 240
           DK KNSDS SDSELSDTK KK++LKSSGSR RSRKSS SD+ESGS  ESESESESEEESR
Sbjct: 181 DKAKNSDSDSDSELSDTKLKKRKLKSSGSRRRSRKSSLSDSESGSDTESESESESEEESR 240

Query: 241 RRRKKSKNRRRNRKHKN-SSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRTR 300
           +RRKKS N RRNRKHK+  SSKKKKS YSDTED EESETDDSD SD V+S+KR R KR++
Sbjct: 241 KRRKKSTN-RRNRKHKSIRSSKKKKSRYSDTEDSEESETDDSDASDHVKSRKRSRRKRSK 300

Query: 301 SSRKRRYSDSDTDEGEESEGRKLRK---SKTSSKSRSKRKRQSETESKSPASEKNSGSED 360
           + RKRRYSDS  DE E SEG KLRK   S TS+KSRSK+ RQSETESK  +SE+NSGSED
Sbjct: 301 NGRKRRYSDS--DESENSEGEKLRKRKSSSTSTKSRSKKNRQSETESKGSSSEENSGSED 360

Query: 361 VDANSKLKVDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGAL 420
           VD  SKLK+DG+KM E+NAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGAL
Sbjct: 361 VDGKSKLKIDGDKMAEINAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGAL 420

Query: 421 RPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQV 480
           RPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQ FETLGYVMSGSRHQRMNAIRIRKENQV
Sbjct: 421 RPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQNFETLGYVMSGSRHQRMNAIRIRKENQV 480

Query: 481 YSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 522
           YSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA
Sbjct: 481 YSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 526

BLAST of MS000012 vs. NCBI nr
Match: XP_022934812.1 (NF-kappa-B-activating protein-like [Cucurbita moschata])

HSP 1 Score: 765.4 bits (1975), Expect = 3.2e-217
Identity = 443/529 (83.74%), Postives = 479/529 (90.55%), Query Frame = 0

Query: 1   MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDY-RRRSPSYESYDRYNHRRRSDSP 60
           MGRLGSTVEIPERRHRSDRE+ +HRY+PDSDASGGDY RRRSPSYESYDRY+HRRRS SP
Sbjct: 1   MGRLGSTVEIPERRHRSDRENGSHRYSPDSDASGGDYRRRRSPSYESYDRYSHRRRSVSP 60

Query: 61  RYHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKR 120
            Y ++RRSPR+DGD+NGLPKRFGRAGGRAYLDRNGR S+ES+SDEELKGLNYE++RRLKR
Sbjct: 61  GYQEIRRSPRADGDQNGLPKRFGRAGGRAYLDRNGRASDESDSDEELKGLNYEDFRRLKR 120

Query: 121 QKLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCG-DDGGYRSGSGEIKQHEEKYES 180
           QKLRKTLKHCIWRVTPSPPRN N+D+E+K DEIL+K G DDGG  SG  E KQHE+KY S
Sbjct: 121 QKLRKTLKHCIWRVTPSPPRNGNEDYEDKDDEILEKYGNDDGGDISGLSEKKQHEKKYAS 180

Query: 181 DKTKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTESGS--ESESESESEEESR 240
           DK KNSDS SDSELSDTK K+++LKSSGSR RSRKSS SD+ESGS  ESESESESEEESR
Sbjct: 181 DKAKNSDSDSDSELSDTKLKRRKLKSSGSRRRSRKSSLSDSESGSDTESESESESEEESR 240

Query: 241 RRRKKSKNRRRNRKHKN-SSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRTR 300
           +RRKKS N RRNRKHK+  SSKKKKS YSDTED EES+TDDSD SD V+S+KR R KR++
Sbjct: 241 KRRKKSTN-RRNRKHKSIRSSKKKKSRYSDTEDSEESKTDDSDASDHVKSRKRSRRKRSK 300

Query: 301 SSRKRRYSDSDTDEGEESEGRKLRK---SKTSSKSRSKRKRQSETESKSPASEKNSGSED 360
           + RKRRYSDS  DE E SEG KLRK   S TS+KSRSK+ RQSETESK  +SE+NSGSED
Sbjct: 301 NGRKRRYSDS--DESENSEGEKLRKRKSSSTSTKSRSKKNRQSETESKGSSSEENSGSED 360

Query: 361 VDANSKLKVDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGAL 420
           VD  SKLK+DG+KM E+NAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGAL
Sbjct: 361 VDGKSKLKIDGDKMAEINAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGAL 420

Query: 421 RPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQV 480
           RPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQ FETLGYVMSGSRHQRMNAIRIRKENQV
Sbjct: 421 RPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQNFETLGYVMSGSRHQRMNAIRIRKENQV 480

Query: 481 YSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 522
           YSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA
Sbjct: 481 YSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 526

BLAST of MS000012 vs. ExPASy Swiss-Prot
Match: Q8N5F7 (NF-kappa-B-activating protein OS=Homo sapiens OX=9606 GN=NKAP PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 5.4e-26
Identity = 153/483 (31.68%), Postives = 222/483 (45.96%), Query Frame = 0

Query: 27  TPDSDASGGDYRRRSPSYESYDRYNHRR-RSDSPRYHDVRRSPRSDGDRNGLPKRFG--R 86
           +PD +ASG   RRRS S       + R  R    R H   RS    GDRNGL  + G   
Sbjct: 9   SPDREASGSGGRRRSSSKSPKPSKSARSPRGRRSRSHSCSRS----GDRNGLTHQLGGLS 68

Query: 87  AGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKRQKLRKTLKHCIWRVTPSPPRNDND 146
            G R    R+  +S   E     +G+    +          +         P P   D +
Sbjct: 69  QGSRNQSYRSRSRSRSRERPSAPRGI---PFASASSSVYYGSYSRPYGSDKPWPSLLDKE 128

Query: 147 DFEEKADEILDKCGDDGGYRSGSGEIKQHEEKYESDKTKNSDSGSDSELSDTKSKKKRLK 206
             E    + L +       R   GE+   E    S K    DS   + + D + KK    
Sbjct: 129 REESLRQKRLSE-------RERIGELGAPEVWGLSPKNPEPDSDEHTPVEDEEPKK---- 188

Query: 207 SSGSRHRSRKSSRSDTESGSESESESESEEESRRRRKKSKNRRRNRKHKNSSSKKKKSMY 266
                                + S S SEEE +++  +SK R + R+ K SS +K K   
Sbjct: 189 --------------------STTSASTSEEEKKKKSSRSKERSKKRRKKKSSKRKHKKYS 248

Query: 267 SDTEDGEESETDDSDVSDRVRSKKRGRSKRTRSSRKRRYSDSDTDEGEESEGRKLRKSKT 326
            D++   +SETD SD  ++ R+KK                       ++ E +K  +SK 
Sbjct: 249 EDSDSDSDSETDSSDEDNKRRAKK----------------------AKKKEKKKKHRSKK 308

Query: 327 SSKSRSKRKRQSETESKSPASEKNSGSEDVDANSKLKVDGEKMTEVNAEALKIKEILEAQ 386
             K RSK+ R+  ++S    S K S  E ++   K +   E+ +++           EA 
Sbjct: 309 YKKKRSKKSRKESSDS----SSKESQEEFLENPWKDRTKAEEPSDLIGP--------EAP 368

Query: 387 KKPAFDNEMPVGPMPLPRAEGHISYGGALRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEI 446
           K     ++ P            ++YG AL PGEG A+A+YV+ GKRIPRRGE+GL++EEI
Sbjct: 369 KTLTSQDDKP------------LNYGHALLPGEGAAMAEYVKAGKRIPRRGEIGLTSEEI 407

Query: 447 QTFETLGYVMSGSRHQRMNAIRIRKENQVYSAEDKRALAMYNYEEKAKRERKVMDDLQRL 506
            +FE  GYVMSGSRH+RM A+R+RKENQ+YSA++KRALA +N EE+ KRE K++   + +
Sbjct: 429 ASFECSGYVMSGSRHRRMEAVRLRKENQIYSADEKRALASFNQEERRKRENKILASFREM 407

BLAST of MS000012 vs. ExPASy Swiss-Prot
Match: Q9D0F4 (NF-kappa-B-activating protein OS=Mus musculus OX=10090 GN=Nkap PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.5e-23
Identity = 151/490 (30.82%), Postives = 228/490 (46.53%), Query Frame = 0

Query: 27  TPDSDASGGDYRRRSPSYESYDRYNHRRRSDSPRYHDVRRSPRSDGDRNGLPKRFGRAGG 86
           +P+ +ASG   +RRSP             S SP+     RSPR    R+    RFG    
Sbjct: 9   SPEREASGA--KRRSP-------------SRSPKSIKSSRSPRCRRSRSRSCSRFG---- 68

Query: 87  RAYLDRNGRQSEESESDEELKGLNYEEYRRLKRQKLRKTLKHCIWRVTPSPPRNDNDDFE 146
               DRNG     S   +  +  +Y    R + ++     +   +    S          
Sbjct: 69  ----DRNGLSHSLSGFSQSSRNQSYRSRSRSRSRERPSAQRSAPFASASSSAYYGGYSRP 128

Query: 147 EKADE----ILDKCGDDG------GYRSGSGEIKQHEEKYESDKTKNSDSGSDSELSDTK 206
              D+    +LDK  ++         R   GE+   E    S K    DS   + + D +
Sbjct: 129 YGGDKPWPSLLDKEREESLRQKRLSERERIGELGAPEVWGLSPKNPEPDSDEHTPVEDEE 188

Query: 207 SKKKRLKSSGSRHRSRKSSRSDTESGSESESESESEEESRRRRKKSKNRRRNRKHKNSSS 266
            KK                       + S S SE +++ +R+   SK+R + ++ K SS 
Sbjct: 189 PKK----------------------STTSASSSEDDKKKKRKSSHSKDRAKKKRKKKSSK 248

Query: 267 KKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRTRSSRKRRYSDSDTDEGEESEGR 326
           +K K    D++   ES+TD SD   + R+KK   +K+    +KRR             G+
Sbjct: 249 RKHKKYSEDSDSDSESDTDSSDEDSKRRAKK---AKKKDKKKKRR-------------GK 308

Query: 327 KLRKSKTSSKSRSKRKRQSETESKSPASEKNSGSEDVDANSKLKVDGEKMTEVNAEALKI 386
           K +K K      SK+ R+  ++S    S K S  E ++   K +   E+ +++       
Sbjct: 309 KYKKKK------SKKNRKESSDS----SSKESQEEFLENPWKDRSKAEEPSDLIGP---- 368

Query: 387 KEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGALRPGEGDAIAQYVQQGKRIPRRGEV 446
               EA K  A  ++ P            ++YG AL PGEG A+A+YV+ GKRIPRRGE+
Sbjct: 369 ----EAPKTLASQDDKP------------LNYGHALLPGEGAAMAEYVKAGKRIPRRGEI 407

Query: 447 GLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQVYSAEDKRALAMYNYEEKAKRERKV 506
           GL++EEI +FE  GYVMSGSRH+RM A+R+RKENQ+YSA++KRALA +N EE+ KRE K+
Sbjct: 429 GLTSEEIASFECSGYVMSGSRHRRMEAVRLRKENQIYSADEKRALASFNQEERRKRENKI 407

BLAST of MS000012 vs. ExPASy Swiss-Prot
Match: Q4V7C9 (NF-kappa-B-activating protein OS=Rattus norvegicus OX=10116 GN=Nkap PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 9.6e-23
Identity = 145/477 (30.40%), Postives = 220/477 (46.12%), Query Frame = 0

Query: 40  RSPSYESYDRYNHRRRSDSPRYHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEE 99
           RSP  E+      R  S SP+     RSPR    R+    RFG        DRNG     
Sbjct: 8   RSPVREA-SGGKRRSSSRSPKSIKSSRSPRCRRSRSRSCSRFG--------DRNGLSHSL 67

Query: 100 SESDEELKGLNYEEYRRLKRQKLRKTLKHCIWRVTPSPPRNDNDDFEEKADE----ILDK 159
           S   +  +  +Y    R + ++     +   +  + S             D+    +LDK
Sbjct: 68  SGFSQSSRNQSYRSRSRSRSRERPSAQRSAPFASSSSSAYYGGYSRPYGGDKPWPSLLDK 127

Query: 160 CGDDG------GYRSGSGEIKQHEEKYESDKTKNSDSGSDSELSDTKSKKKRLKSSGSRH 219
             ++         R   GE+   E    S K    DS   + + D + KK          
Sbjct: 128 EREESLRQKRLSERERIGELGAPEVWGLSPKNPEPDSDEHTPVEDEEPKK---------- 187

Query: 220 RSRKSSRSDTESGSESESESESEEESRRRRKKSKNRRRNRKHKNSSSKKKKSMYSDTEDG 279
                        + S S SE +++ +R+  +SK R + ++ K SS +K K    D++  
Sbjct: 188 ------------STTSASSSEDDKKKKRKSSRSKERAKKKRKKKSSKRKHKKYSEDSDSD 247

Query: 280 EESETDDSDVSDRVRSKKRGRSKRTRSSRKRRYSDSDTDEGEESEGRKLRKSKTSSKSRS 339
            ES+TD SD   + R+KK                       ++ E +K R+ K   K +S
Sbjct: 248 SESDTDSSDEDSKRRAKK----------------------AKKKEKKKKRRGKKYKKKKS 307

Query: 340 KRKRQSETESKSPASEKNSGSEDVDANSKLKVDGEKMTEVNAEALKIKEILEAQKKPAFD 399
           K+ R+  ++S    S K S  E ++   K +   E+ +++           EA K  A  
Sbjct: 308 KKNRKESSDS----SSKESQEEFLENPWKDRSKTEEPSDLIGP--------EAPKTLASQ 367

Query: 400 NEMPVGPMPLPRAEGHISYGGALRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETL 459
           ++ P            ++YG AL PGEG A+A+YV+ GKRIPRRGE+GL++EEI +FE  
Sbjct: 368 DDKP------------LNYGHALLPGEGAAMAEYVKAGKRIPRRGEIGLTSEEIASFECS 407

Query: 460 GYVMSGSRHQRMNAIRIRKENQVYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQR 507
           GYVMSGSRH+RM A+R+RKENQ+YSA++KRALA +N EE+ KRE K++   + +V R
Sbjct: 428 GYVMSGSRHRRMEAVRLRKENQIYSADEKRALASFNQEERRKRENKILASFREMVYR 407

BLAST of MS000012 vs. ExPASy Swiss-Prot
Match: Q4P4G8 (NKAP family protein UM04995 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=UMAG_04995 PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 3.7e-22
Identity = 150/469 (31.98%), Postives = 223/469 (47.55%), Query Frame = 0

Query: 53  RRRSDSPRYHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYE 112
           RRR D     + RR+ + D  R     R G    R Y D N  ++   E  E        
Sbjct: 142 RRRDDQRWQANERRANQDDSRRAWTSSRSG--DRRRYFDSNDDRNPRQEDREAPSEYGAA 201

Query: 113 EYRRLKRQKLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCGDDGGYRSGSGEIKQH 172
           +++RL+R     ++         S   N  D             G+ G + S + + K+ 
Sbjct: 202 QWKRLQRDPYASSM---------SSGGNQGD-------------GNGGFFSSRNEQRKKS 261

Query: 173 EEKYESDKTKNSDSGSDSELSDTKSKKKRLKSSGSRHRSR-KSSRSDTESGSESESESES 232
                     +    SD E    + K+KR + S SRHR + K    D + G      S S
Sbjct: 262 TISIWPPSPPHPTLDSDEE----REKRKRHRDSSSRHRDKDKDKDRDKDKGKRDRKHSSS 321

Query: 233 EE---ESRRRRKKSKNRRRNRKHKNSSSKK-------KKSMYSDTEDGEESETDDSDVSD 292
           ++    SR  R  S   RR R  ++S   K       + S     +D ++ + +D DV  
Sbjct: 322 DKHHSSSRSHRHHSSTSRRRRSSRHSEDDKASRHRHTRSSRSHHRDDDDDDDDEDVDVDG 381

Query: 293 RVRSKKRGRSKRTRSSRKRRYSDSDTD-EGEESEGRKLRKSKTSSKSRSKRKRQSETESK 352
           R R  KR R+K +  S   R S+S+TD + +    R  R+   S +S + R+R+SE   +
Sbjct: 382 R-RKSKRSRTKVSDGSDSGR-SESETDSDSDARSSRHRRRHHKSDRSSTHRRRESEKRHR 441

Query: 353 SPASEKNSGSEDVDANSKLKVDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGP-MPL 412
           S      S S     +++ +    +   V+A               A D+++ VGP +P 
Sbjct: 442 SSGQRSESESSLHSEHNQKETAKSRRDSVSAS--------------ASDSDVEVGPTLPT 501

Query: 413 PRAEGH----ISYGGALRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSG 472
             A+G      +YGGAL PGEG A+A YVQ GKRIPRRGE+GL++++I+ +E  GYVMSG
Sbjct: 502 VAADGKPVDPRAYGGALLPGEGSAMASYVQDGKRIPRRGEIGLTSDQIEAYEKAGYVMSG 561

Query: 473 SRHQRMNAIRIRKENQVYSAEDKRALAMYNYEEKAKRERKVMDDLQRLV 505
           SRH RMNA+R+RKENQV SAE+KR +     EEKAK+ER+++   + LV
Sbjct: 562 SRHHRMNAVRMRKENQVISAEEKRTMLRLQAEEKAKKEREIVSQFKELV 566

BLAST of MS000012 vs. ExPASy Swiss-Prot
Match: Q55ED4 (NKAP family protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0269284 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 2.2e-19
Identity = 159/534 (29.78%), Postives = 252/534 (47.19%), Query Frame = 0

Query: 12  ERRHRSDRESAAHRYTPDSDASGGDYRRRSPSYESYDR-----YNHRRRSDSPRYHDVR- 71
           +R   SDR+   +RY+  S + G   R RS S  S DR     YN  R S+   Y++ R 
Sbjct: 9   DRDRDSDRDRDRNRYS-RSRSRGSRSRSRSRS-RSRDRNRNRDYNKDRSSNRDSYYNDRD 68

Query: 72  -RSPRS--------DGDRNGLPKR------FGRAGGRAYLDRNGRQSEESESDEELKGLN 131
            +  RS        D DRN   K        G  GG +  +   + S   ES+     + 
Sbjct: 69  YKKDRSSNRDRDYYDRDRNRDYKNSSSGSSSGGGGGGSGENYTVKSSSYRESNSNNSKVE 128

Query: 132 YEEYRRLKRQKL--RKTLKHCIWRV-TPSPPRNDNDDFEEKADEILDKC---------GD 191
            ++   + +      K + + I R        N+N+ F+ +  E  +            +
Sbjct: 129 IQKQENISKDYFIDNKGVNNSIERKDNIKNENNNNNSFDNRKAEFNNNSYGEKKEFDNNN 188

Query: 192 DGGYRSGSGEIKQHEEKYESDKTKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSD 251
           +  Y  G+     +     ++   N+++ S+   +D    +K+L+   +     + S S 
Sbjct: 189 NRNYHGGNSRYVNNNNNNNNNNYNNNNNKSNYNGTDFFEYRKKLREEATCFSVYERSPSP 248

Query: 252 TESGSESESESESEEESRRRRKKSKNRRRNRKHKNSSSKKKKSMYSDTEDGEESETDDSD 311
            + G   E E   ++    ++K  K+RR++  + +SSS       SD+    E       
Sbjct: 249 PKKG---ELEEIDDQFLSNKKKSKKSRRKSSSNSDSSSSDSD---SDSSRSREKRKKSKS 308

Query: 312 VSDRVRSKKRGRSKRTRSSRKRRYSDSDTD-EGEESEGRKLRKSKTSSKSRSKRKRQSET 371
             D+ + K++ + +R  S R  +YSDSD+D +   S+       ++ S+ RS++KR  + 
Sbjct: 309 RKDKKKRKEKKKHQRKSSKRSSKYSDSDSDSDSSFSDSDYSDSDRSDSEGRSRKKRSKKR 368

Query: 372 ESKSPASEKNSGSEDVDANSKLKVDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGPM 431
             K     K S            V    M E   E          Q+     N   +GP 
Sbjct: 369 SKKRHDHHKES------------VHDASMWEEKVE----------QQTQTLSNR-DIGPK 428

Query: 432 PLPRAEGHISYGGALRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSR 491
           P+   +   SYGGA+ PGE +AIAQ+V++ KRIPRRGEVGL++E+I +FE  GYVMSGSR
Sbjct: 429 PISEVQVG-SYGGAMMPGEAEAIAQFVKENKRIPRRGEVGLTSEQIASFEETGYVMSGSR 488

Query: 492 HQRMNAIRIRKENQVYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHD 512
           H+RMNA+RIRKE+QVYSAE+++ALAM N EEKAKRE +++ D + L+   +  D
Sbjct: 489 HRRMNAVRIRKESQVYSAEEQKALAMLNREEKAKRENRLLADFRDLINTKLQAD 510

BLAST of MS000012 vs. ExPASy TrEMBL
Match: A0A6J1DF44 (NF-kappa-B-activating protein OS=Momordica charantia OX=3673 GN=LOC111019852 PE=3 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 2.3e-269
Identity = 519/521 (99.62%), Postives = 520/521 (99.81%), Query Frame = 0

Query: 1   MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDYRRRSPSYESYDRYNHRRRSDSPR 60
           MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDYRRRSPSYESYDRYNHRRRSDSPR
Sbjct: 1   MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDYRRRSPSYESYDRYNHRRRSDSPR 60

Query: 61  YHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKRQ 120
           YHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKRQ
Sbjct: 61  YHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKRQ 120

Query: 121 KLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCGDDGGYRSGSGEIKQHEEKYESDK 180
           KLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCGDDGGYRSGSGEIKQHEEKYESDK
Sbjct: 121 KLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCGDDGGYRSGSGEIKQHEEKYESDK 180

Query: 181 TKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTESGSESESESESEEESRRRRK 240
           TKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTESGS SESESESEEESRRRRK
Sbjct: 181 TKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTESGSASESESESEEESRRRRK 240

Query: 241 KSKNRRRNRKHKNSSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRTRSSRKR 300
           KSKNRRRNRKHKNSSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRTRSSRKR
Sbjct: 241 KSKNRRRNRKHKNSSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRTRSSRKR 300

Query: 301 RYSDSDTDEGEESEGRKLRKSKTSSKSRSKRKRQSETESKSPASEKNSGSEDVDANSKLK 360
           RYSDSDTDEGEESEGRKLRKSKTSSKSRSKRKRQSETESKSPASE+NSGSEDVDANSKLK
Sbjct: 301 RYSDSDTDEGEESEGRKLRKSKTSSKSRSKRKRQSETESKSPASEENSGSEDVDANSKLK 360

Query: 361 VDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGALRPGEGDAI 420
           VDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGALRPGEGDAI
Sbjct: 361 VDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGALRPGEGDAI 420

Query: 421 AQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQVYSAEDKRA 480
           AQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQVYSAEDKRA
Sbjct: 421 AQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQVYSAEDKRA 480

Query: 481 LAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 522
           LAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA
Sbjct: 481 LAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 521

BLAST of MS000012 vs. ExPASy TrEMBL
Match: A0A6J1F3U7 (NF-kappa-B-activating protein-like OS=Cucurbita moschata OX=3662 GN=LOC111441871 PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.6e-217
Identity = 443/529 (83.74%), Postives = 479/529 (90.55%), Query Frame = 0

Query: 1   MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDY-RRRSPSYESYDRYNHRRRSDSP 60
           MGRLGSTVEIPERRHRSDRE+ +HRY+PDSDASGGDY RRRSPSYESYDRY+HRRRS SP
Sbjct: 1   MGRLGSTVEIPERRHRSDRENGSHRYSPDSDASGGDYRRRRSPSYESYDRYSHRRRSVSP 60

Query: 61  RYHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKR 120
            Y ++RRSPR+DGD+NGLPKRFGRAGGRAYLDRNGR S+ES+SDEELKGLNYE++RRLKR
Sbjct: 61  GYQEIRRSPRADGDQNGLPKRFGRAGGRAYLDRNGRASDESDSDEELKGLNYEDFRRLKR 120

Query: 121 QKLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCG-DDGGYRSGSGEIKQHEEKYES 180
           QKLRKTLKHCIWRVTPSPPRN N+D+E+K DEIL+K G DDGG  SG  E KQHE+KY S
Sbjct: 121 QKLRKTLKHCIWRVTPSPPRNGNEDYEDKDDEILEKYGNDDGGDISGLSEKKQHEKKYAS 180

Query: 181 DKTKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTESGS--ESESESESEEESR 240
           DK KNSDS SDSELSDTK K+++LKSSGSR RSRKSS SD+ESGS  ESESESESEEESR
Sbjct: 181 DKAKNSDSDSDSELSDTKLKRRKLKSSGSRRRSRKSSLSDSESGSDTESESESESEEESR 240

Query: 241 RRRKKSKNRRRNRKHKN-SSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRTR 300
           +RRKKS N RRNRKHK+  SSKKKKS YSDTED EES+TDDSD SD V+S+KR R KR++
Sbjct: 241 KRRKKSTN-RRNRKHKSIRSSKKKKSRYSDTEDSEESKTDDSDASDHVKSRKRSRRKRSK 300

Query: 301 SSRKRRYSDSDTDEGEESEGRKLRK---SKTSSKSRSKRKRQSETESKSPASEKNSGSED 360
           + RKRRYSDS  DE E SEG KLRK   S TS+KSRSK+ RQSETESK  +SE+NSGSED
Sbjct: 301 NGRKRRYSDS--DESENSEGEKLRKRKSSSTSTKSRSKKNRQSETESKGSSSEENSGSED 360

Query: 361 VDANSKLKVDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGAL 420
           VD  SKLK+DG+KM E+NAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGAL
Sbjct: 361 VDGKSKLKIDGDKMAEINAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGAL 420

Query: 421 RPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQV 480
           RPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQ FETLGYVMSGSRHQRMNAIRIRKENQV
Sbjct: 421 RPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQNFETLGYVMSGSRHQRMNAIRIRKENQV 480

Query: 481 YSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 522
           YSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA
Sbjct: 481 YSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 526

BLAST of MS000012 vs. ExPASy TrEMBL
Match: A0A6J1J8D5 (NF-kappa-B-activating protein-like OS=Cucurbita maxima OX=3661 GN=LOC111482277 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.5e-215
Identity = 442/529 (83.55%), Postives = 474/529 (89.60%), Query Frame = 0

Query: 1   MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDY-RRRSPSYESYDRYNHRRRSDSP 60
           MGRLGSTVEIPERRHRSDRE+ +HRY+PDSDASGGDY RRRSPSYESYDRY+HRRRS SP
Sbjct: 1   MGRLGSTVEIPERRHRSDRENGSHRYSPDSDASGGDYRRRRSPSYESYDRYSHRRRSVSP 60

Query: 61  RYHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKR 120
            Y D+RRSPR+DGD+NGLPKRFGRAGGRAYLDRNGR SEES+SDEELKGLNYE+YRR+KR
Sbjct: 61  GYQDIRRSPRADGDQNGLPKRFGRAGGRAYLDRNGRASEESDSDEELKGLNYEDYRRMKR 120

Query: 121 QKLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCG-DDGGYRSGSGEIKQHEEKYES 180
           QKLRKTLKHCIWRVTPSPPRN N+D+E+K DEIL+K G DDGG  SG  E KQHE+KY S
Sbjct: 121 QKLRKTLKHCIWRVTPSPPRNGNEDYEDKDDEILEKYGNDDGGDISGLSEKKQHEKKYAS 180

Query: 181 DKTKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTESGS--ESESESESEEESR 240
           DKTKNSDS SDSELSDTK K ++LKSSGSR RSRK S SD+ESGS  ESESESESEEESR
Sbjct: 181 DKTKNSDSDSDSELSDTKLKGRKLKSSGSRRRSRKLSLSDSESGSDTESESESESEEESR 240

Query: 241 RRRKKSKNRRRNRKHKN-SSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRTR 300
           +RRKKS N RRNRKHK+  SSKKKKS YSD ED EESETDDSD S  V+S+KR   KR++
Sbjct: 241 KRRKKSMN-RRNRKHKSIRSSKKKKSRYSDMEDSEESETDDSDASGHVKSRKRSHRKRSK 300

Query: 301 SSRKRRYSDSDTDEGEESEGRKLRKSK---TSSKSRSKRKRQSETESKSPASEKNSGSED 360
           + RKRRYSDS  DE E SE  KLRK K   TS+KSRSK+ RQSETESK  +SE+NS SED
Sbjct: 301 NGRKRRYSDS--DESENSEAEKLRKRKSSLTSTKSRSKKNRQSETESKGSSSEENSDSED 360

Query: 361 VDANSKLKVDGEKMTEVNAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGAL 420
           VDA SKLK+DG+KM E+NAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGAL
Sbjct: 361 VDAKSKLKIDGDKMAEINAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHISYGGAL 420

Query: 421 RPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQV 480
           RPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQ FETLGYVMSGSRHQRMNAIRIRKENQV
Sbjct: 421 RPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQNFETLGYVMSGSRHQRMNAIRIRKENQV 480

Query: 481 YSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 522
           YSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA
Sbjct: 481 YSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 526

BLAST of MS000012 vs. ExPASy TrEMBL
Match: A0A5D3DPM0 (NF-kappa-B-activating protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006460 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.8e-213
Identity = 447/534 (83.71%), Postives = 481/534 (90.07%), Query Frame = 0

Query: 1   MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDY-RRRSPSYESYDRYNHRRRSDSP 60
           MGRLGSTVEIPERRHRSDRE+  HRY+PDSDAS GDY RRRSPSYESYDRYN+RRRS SP
Sbjct: 1   MGRLGSTVEIPERRHRSDRENGTHRYSPDSDASRGDYRRRRSPSYESYDRYNNRRRSVSP 60

Query: 61  RYHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKR 120
            Y +VRRSPR+DGD+NGLPKRFGRAGGRAYLDRNGR S+ES+SDEELKGLNYEEYRRLKR
Sbjct: 61  GYENVRRSPRADGDQNGLPKRFGRAGGRAYLDRNGRASDESDSDEELKGLNYEEYRRLKR 120

Query: 121 QKLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCG----DDGGYRSGSGEIKQHEEK 180
           QKLRK+LKHCIWRVTPSPPRN N+++EEK D+IL+K G     DGG +SG  E KQ EEK
Sbjct: 121 QKLRKSLKHCIWRVTPSPPRNGNEEYEEKYDDILEKYGGDGDGDGGDKSGLNEKKQLEEK 180

Query: 181 YESDKTKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTE--SGSESESESESEE 240
           Y SDKTKNSDS SDSELSD K+++++ KSSGSR RSRKSS SD+E  S +ESESESESEE
Sbjct: 181 YVSDKTKNSDSDSDSELSDRKTERRKSKSSGSRRRSRKSSPSDSESYSNTESESESESEE 240

Query: 241 ESRRRRKKSKNRRRNRKHKN-SSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSK 300
           ESRRRRKKSK+ RR+RKHKN  SSKKKKS YSDTED EESETDDSDVSD V+S+KR RSK
Sbjct: 241 ESRRRRKKSKS-RRSRKHKNIKSSKKKKSRYSDTEDSEESETDDSDVSDHVKSRKRSRSK 300

Query: 301 RTRSSRKRRYSDSDTDEGEESEGRKLRKSK---TSSKSRSKRKRQSETESKSPAS-EKNS 360
           R+++SRKRRYSDS  DE E+SEG KLRK K   TSSKSRSKRKRQSETESKS +S E+NS
Sbjct: 301 RSKNSRKRRYSDS--DESEDSEGEKLRKRKSSLTSSKSRSKRKRQSETESKSCSSAEENS 360

Query: 361 GSEDVDANSKLKVDGEKMTEVN-AEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHIS 420
           GSED+D  SK  VDGEKM E+N AEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHIS
Sbjct: 361 GSEDIDDKSKSMVDGEKMAEINAAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHIS 420

Query: 421 YGGALRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIR 480
           YGGALRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIR
Sbjct: 421 YGGALRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIR 480

Query: 481 KENQVYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 522
           KENQVYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA
Sbjct: 481 KENQVYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 531

BLAST of MS000012 vs. ExPASy TrEMBL
Match: A0A1S3B8B4 (NF-kappa-B-activating protein OS=Cucumis melo OX=3656 GN=LOC103486869 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.8e-213
Identity = 447/534 (83.71%), Postives = 481/534 (90.07%), Query Frame = 0

Query: 1   MGRLGSTVEIPERRHRSDRESAAHRYTPDSDASGGDY-RRRSPSYESYDRYNHRRRSDSP 60
           MGRLGSTVEIPERRHRSDRE+  HRY+PDSDAS GDY RRRSPSYESYDRYN+RRRS SP
Sbjct: 1   MGRLGSTVEIPERRHRSDRENGTHRYSPDSDASRGDYRRRRSPSYESYDRYNNRRRSVSP 60

Query: 61  RYHDVRRSPRSDGDRNGLPKRFGRAGGRAYLDRNGRQSEESESDEELKGLNYEEYRRLKR 120
            Y +VRRSPR+DGD+NGLPKRFGRAGGRAYLDRNGR S+ES+SDEELKGLNYEEYRRLKR
Sbjct: 61  GYENVRRSPRADGDQNGLPKRFGRAGGRAYLDRNGRASDESDSDEELKGLNYEEYRRLKR 120

Query: 121 QKLRKTLKHCIWRVTPSPPRNDNDDFEEKADEILDKCG----DDGGYRSGSGEIKQHEEK 180
           QKLRK+LKHCIWRVTPSPPRN N+++EEK D+IL+K G     DGG +SG  E KQ EEK
Sbjct: 121 QKLRKSLKHCIWRVTPSPPRNGNEEYEEKYDDILEKYGGDGDGDGGDKSGLNEKKQLEEK 180

Query: 181 YESDKTKNSDSGSDSELSDTKSKKKRLKSSGSRHRSRKSSRSDTE--SGSESESESESEE 240
           Y SDKTKNSDS SDSELSD K+++++ KSSGSR RSRKSS SD+E  S +ESESESESEE
Sbjct: 181 YVSDKTKNSDSDSDSELSDRKTERRKSKSSGSRRRSRKSSPSDSESYSNTESESESESEE 240

Query: 241 ESRRRRKKSKNRRRNRKHKN-SSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSK 300
           ESRRRRKKSK+ RR+RKHKN  SSKKKKS YSDTED EESETDDSDVSD V+S+KR RSK
Sbjct: 241 ESRRRRKKSKS-RRSRKHKNIKSSKKKKSRYSDTEDSEESETDDSDVSDHVKSRKRSRSK 300

Query: 301 RTRSSRKRRYSDSDTDEGEESEGRKLRKSK---TSSKSRSKRKRQSETESKSPAS-EKNS 360
           R+++SRKRRYSDS  DE E+SEG KLRK K   TSSKSRSKRKRQSETESKS +S E+NS
Sbjct: 301 RSKNSRKRRYSDS--DESEDSEGEKLRKRKSSLTSSKSRSKRKRQSETESKSCSSAEENS 360

Query: 361 GSEDVDANSKLKVDGEKMTEVN-AEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHIS 420
           GSED+D  SK  VDGEKM E+N AEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHIS
Sbjct: 361 GSEDIDDKSKSMVDGEKMAEINAAEALKIKEILEAQKKPAFDNEMPVGPMPLPRAEGHIS 420

Query: 421 YGGALRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIR 480
           YGGALRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIR
Sbjct: 421 YGGALRPGEGDAIAQYVQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIR 480

Query: 481 KENQVYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 522
           KENQVYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA
Sbjct: 481 KENQVYSAEDKRALAMYNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 531

BLAST of MS000012 vs. TAIR 10
Match: AT4G02720.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF926 (InterPro:IPR009269); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 338.2 bits (866), Expect = 1.2e-92
Identity = 243/458 (53.06%), Postives = 304/458 (66.38%), Query Frame = 0

Query: 75  NGLPKRFGRAGGRAYLD---RNGRQSEESESDEELKGLNYEEYRRLKRQKLRKTLKHCIW 134
           N LP+RFG+  G  YLD   RNGR+S  S+SDEELKGL++EEYRR KR K+RK+ K C W
Sbjct: 4   NSLPRRFGKNHG--YLDRDYRNGRRS-GSDSDEELKGLSHEEYRRQKRLKMRKSAKFCFW 63

Query: 135 RVTPSPPRNDNDDFEEKADEILDKCGDDGGYRSGSGEIKQHEEKYESDKTKNSDSGSDSE 194
             TPSPPR+ N+D +E ADEI DK   +GG R  + + K+ + K          S S+SE
Sbjct: 64  ENTPSPPRDQNEDSDENADEIQDK---NGGERDDNSKGKERKGK----------SDSESE 123

Query: 195 LSDTKSKKKRLKSSGSRHRSRKSSRSDTESGSESESESESEEESRRRRKKSKNRRRNRKH 254
               +S+K++ KSS S+ R ++S  SD+ES     SES+SEEE RRRR+KS ++R+ +  
Sbjct: 124 SDGLRSRKRKSKSSRSKRRRKRSYDSDSES---EGSESDSEEEDRRRRRKSSSKRK-KSR 183

Query: 255 KNSSSKKKKSMYSDTEDGEESETDDSDVSDRVRSKKRGRSKRTRSSRKRRYSDSDTDEGE 314
            + S +KK+S    T+  +  E+ D D    + +   G  + T+S  KRR          
Sbjct: 184 SSRSFRKKRSHRRKTKYSDSDESSDEDSKAEISASSSGEEEDTKSKSKRR---------- 243

Query: 315 ESEGRKLRKSKTSSKSRSKRKRQSETESKSPASEKNSGSEDVDANSKLKVDGE-KMTEV- 374
                     K SS S SKR +  +T+S        S S+  + +SK++VD   K TE+ 
Sbjct: 244 ----------KKSSDSSSKRSKGEKTKS-------GSDSDGTEEDSKMQVDETVKNTELE 303

Query: 375 --NAEALKIKEILEAQKKPAF----DNEMPVGPMPLPRAEGHISYGGALRPGEGDAIAQY 434
               E  K KE++E +KK +     + E  VGPMPLP+AEGHISYGGALRPGEGDAIAQY
Sbjct: 304 LDEEELKKFKEMIELKKKSSAVDEEEEEGDVGPMPLPKAEGHISYGGALRPGEGDAIAQY 363

Query: 435 VQQGKRIPRRGEVGLSAEEIQTFETLGYVMSGSRHQRMNAIRIRKENQVYSAEDKRALAM 494
           VQQGKRIPRRGEVGL+AEEIQ FE LGYVMSGSRHQRMNAIRIRKENQVYSAEDKRALAM
Sbjct: 364 VQQGKRIPRRGEVGLNAEEIQKFEDLGYVMSGSRHQRMNAIRIRKENQVYSAEDKRALAM 414

Query: 495 YNYEEKAKRERKVMDDLQRLVQRHIGHDVGPSHDPFAA 522
           +NYEEKAKRE KVM DLQRLVQRH+G +VGP+HDPF A
Sbjct: 424 FNYEEKAKREAKVMSDLQRLVQRHMGEEVGPNHDPFGA 414