MELO3C034150.jh1 (gene) Melon (Harukei-3) v1.41

Overview


Name	MELO3C034150.jh1
Type	gene
Organism	Cucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Description	cytochrome P450, family 71, subfamily B, polypeptide 32
Location	chr10: 3846833 .. 3848821 (-)
RNA-Seq Expression	MELO3C034150.jh1
Synteny	MELO3C034150.jh1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSstart_codonstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAATACAGAACTTTCAGTCATTACCATTTGGAGGTGGTAGAAGAAACTGTCCTGGTATGAACGTGGGGATTGTTATGATGAAGTTGGTATTGGCTAATTTATTGCCAATTTAATTGGAAATTGCATGATGGGATGAAAGAAGAAGATATGGATATGGAAGAGGATGCTGGTCTAACTGTTGCGAAATTGCCTCTTAAACTCGTTCCAATGACCTATTGTCCTTAGCTTTACTCATCATCAAACTTTTGTTGCTGTAAAAATTGAAGTAATATATATTACAAATTTAGGCTTCATTTGGGATTACTATCATTTACGTAATTAGTGGCAACTGTACTTTTAGTAAAATTAAGTATAATGTAAAACGTATTAGTGTTTTGCTAAATTTCAGGTATACCAAGGATTTAATATCTTTCGAGTTTCCAAGATGCCGAGATGCTGTAAGGTAATACAAGTTTCATGAAAGATAAGTCGAGATGCATGTAAATTTTCTCGAACACACAAATATTTACTTTTTCTTTACTTTGATTAAGATGAAGCACAAAGTTGATGTGTCTTATTTTTATTGAAGATACCGTCAAAAAATCTCATAATGTCATGTCTTTGTTACATTTTACCCATTGGAGACCAAATACGAAATAATTGAGTCAGAATTCATCTATTTTAATGATATCTTATCTGTCCCCCTAGAAGGTAGAAAACTTAAGCAAGTCGCTCAAAGGAAAAAGGAAATAAACAACCAAATTCCACCAAATAATCAGAAGAAGCGAAAGCATGACACTTGGTTTGGACAAGGTCTCAATGTTTTGTTTTACAGTTACTTTTGATTAGAACACCAGTAGGAGGTCCACCAACAAACAATTGGCAATAAGTGTGCATCCAATAAGAGTATCTCAGCATAGTACCAAAATTAGACCTTGCTCTGAAAAGCCCTTTTAGCTCAAGCTTAATCCCATTTGTCTCCAATTGTTTCTGTAGTCTTTGCCTTTCCAAAATTGGTAGTCTCACTTGACTTGATACCATATGAATATTAATCAACATTGATTCTTCTTTGTATGCTGAGAATGGTGCTATGAATTGGGTAGCAATGAGTGTGTTTCCATAGTAAAGATAGAGTATCATTGAGCTGAAATCCACACTCACTTTCTTGTTTGGATTTGTGAAGTTTGCTAGTAAATTCACATCTGCATTTAGTAGATACCCCATGTCGAGATACGCTGCGTTTAGATTGGCTGTTGCTACGTCAAACCGTGGAGATTTCGGGCGGAAGATGAGGTATACAATGAGGATTACTAGTCCTCCAACGATTACAAGCACCCAAAAGATTGCGCAAAAGACTGCTCCAAACCACATTAATGGATTGGTTTGACGGGGTAGAGGCATAAGTAGGATGTTGTTTTGTTGATTTCTTCTGTTGTCATCATCAACGACATCATTCCTTCGATGGCGATAAGGCTCTCGTGAATCAGATGGGTCTTGAATTTGTACTCGAGGACGTGATTGTTGCACATTTTGTTCAGGTGATTTTAAGTTTGGCTCTCCATATGGAACTTGATATTCCTTCTTCAAACGTGGTGCTTTTCCATATGGATCTTGTGGTGCTTTTCCATATGGATCTTGAGGATGGCGCTGCAGTTGTTCACGATGGTCAGATGGCGGCGATTGAGTGGGCGGTGGAGGATACGATGGGTTTTGACGATTTTTCTTCAAACGTGGTGCCTTACCAATAGTAGGTCGTTGTTCTGCCACCGGCCATGGAGTGGTTGGGTCAGAATATGGGTCGGGATCAGGCTGTGGCGGATCAGAAGGGTCTTGAGGCTGTAATGGGGGTTGCTGAGAACGGCGGTCAGGCTGCAACGGCCGCCGGAAATGGGGGTTGGTTTCAGGACGATTCATGTTGGGAAGAAGTGTGCAGCAGAAATGAAAGGGGACAGAATGGAAATGAGTGGAAATTTGATGTTAAGCCCATAAGAAGCTTGCTTTGGTAA

mRNA sequence

ATGGGAATACAGAACTTTCAGTCATTACCATTTGGAGGTGGTAGAAGAAACTGTCCTGGTATGAACGTGGGGATTGTTATGATGAAGTTGGTATACCAAGGATTTAATATCTTTCGAGTTTCCAAGATGCCGAGATGCTGTAAGAACACCAGTAGGAGGTCCACCAACAAACAATTGGCAATAAGTGTGCATCCAATAAGATTTGCTAGTAAATTCACATCTGCATTTAGTAGATACCCCATGTCGAGATACGCTGCGTTTAGATTGGCTGTTGCTACGTCAAACCGTGGAGATTTCGGGCGGAAGATGAGGTATACAATGAGGATTACTAGTCCTCCAACGATTACAAGCACCCAAAAGATTGCGCAAAAGACTGCTCCAAACCACATTAATGGATTGGTGATTTTAAGTTTGGCTCTCCATATGGAACTTGATATTCCTTCTTCAAACGTGGTGCTTTTCCATATGGATCTTGTGGTGCTTTTCCATATGGATCTTGAGGATGGCGCTGCAGTTGTTCACGATGGTCAGATGGCGGCGATTGAGTGGGCGGTGGAGGATACGATGGGTTTTGACGATTTTTCTTCAAACGTGGTGCCTTACCAATAGTAGGTCGTTGTTCTGCCACCGGCCATGGAGTGGTTGGGTCAGAATATGGGTCGGGATCAGGCTGTGGCGGATCAGAAGGGTCTTGAGGCTGTAATGGGGGTTGCTGAGAACGGCGGTCAGGCTGCAACGGCCGCCGGAAATGGGGGTTGGTTTCAGGACGATTCATGTTGGGAAGAAGTGTGCAGCAGAAATGAAAGGGGACAGAATGGAAATGAGTGGAAATTTGATGTTAAGCCCATAAGAAGCTTGCTTTGGTAA

Coding sequence (CDS)

ATGGGAATACAGAACTTTCAGTCATTACCATTTGGAGGTGGTAGAAGAAACTGTCCTGGTATGAACGTGGGGATTGTTATGATGAAGTTGGTATACCAAGGATTTAATATCTTTCGAGTTTCCAAGATGCCGAGATGCTGTAAGAACACCAGTAGGAGGTCCACCAACAAACAATTGGCAATAAGTGTGCATCCAATAAGATTTGCTAGTAAATTCACATCTGCATTTAGTAGATACCCCATGTCGAGATACGCTGCGTTTAGATTGGCTGTTGCTACGTCAAACCGTGGAGATTTCGGGCGGAAGATGAGGTATACAATGAGGATTACTAGTCCTCCAACGATTACAAGCACCCAAAAGATTGCGCAAAAGACTGCTCCAAACCACATTAATGGATTGGTGATTTTAAGTTTGGCTCTCCATATGGAACTTGATATTCCTTCTTCAAACGTGGTGCTTTTCCATATGGATCTTGTGGTGCTTTTCCATATGGATCTTGAGGATGGCGCTGCAGTTGTTCACGATGGTCAGATGGCGGCGATTGAGTGGGCGGTGGAGGATACGATGGGTTTTGACGATTTTTCTTCAAACGTGGTGCCTTACCAATAG

Protein sequence

MGIQNFQSLPFGGGRRNCPGMNVGIVMMKLVYQGFNIFRVSKMPRCCKNTSRRSTNKQLAISVHPIRFASKFTSAFSRYPMSRYAAFRLAVATSNRGDFGRKMRYTMRITSPPTITSTQKIAQKTAPNHINGLVILSLALHMELDIPSSNVVLFHMDLVVLFHMDLEDGAAVVHDGQMAAIEWAVEDTMGFDDFSSNVVPYQ

Homology

[Close]

BLAST of MELO3C034150.jh1 vs. NCBI nr
Match: XP_026432287.1 (cytochrome P450 71B20-like [Papaver somniferum])

HSP 1 Score: 52.4 bits (124), Expect = 1.80e-04
Identity = 28/65 (43.08%), Postives = 38/65 (58.46%), Query Frame = 0


Query: 4   QNFQSLPFGGGRRNCPGMNVGIVMMKLVYQGFNIFRVSKMPRCCKNTSRRSTNKQLAI-- 63
           QNF+ LPFGGGRR CPG+N GIV+++LV          ++P+  K     S  + LA   
Sbjct: 117 QNFEYLPFGGGRRVCPGLNTGIVLVELVLANILCSFDWELPKGLKREDLNSKEEYLAALG 176

Query: 64  SVHPI 66
           S HP+
Sbjct: 177 SRHPL 181

[Close]

BLAST of MELO3C034150.jh1 vs. NCBI nr
Match: XP_026432067.1 (cytochrome P450 71B34-like [Papaver somniferum])

HSP 1 Score: 52.0 bits (123), Expect = 6.37e-04
Identity = 28/75 (37.33%), Postives = 42/75 (56.00%), Query Frame = 0


Query: 1   MGIQNFQSLPFGGGRRNCPGMNVGIVMMKLVYQGFNIFRVSKMPRCCKN-------TSRR 60
           +G +NF+ LPFGGGRR CPG+N+GI+M +L+          KMP   K        +S  
Sbjct: 442 LGGRNFEFLPFGGGRRGCPGVNMGIIMTELILANLLYSFDWKMPNGLKKEDLNMDESSGI 501

Query: 61  STNKQLAISVHPIRF 68
           S  K+  + + PI++
Sbjct: 502 SIYKKFPLELVPIKY 516

[Close]

BLAST of MELO3C034150.jh1 vs. NCBI nr
Match: XP_026383323.1 (cytochrome P450 71A1-like [Papaver somniferum])

HSP 1 Score: 50.8 bits (120), Expect = 7.24e-04
Identity = 21/30 (70.00%), Postives = 26/30 (86.67%), Query Frame = 0


Query: 2   GIQNFQSLPFGGGRRNCPGMNVGIVMMKLV 31
           G QNF+ LPFGGGRR CPGMN+ IV+++LV
Sbjct: 71  GSQNFEYLPFGGGRRGCPGMNMAIVLIELV 100

[Close]

BLAST of MELO3C034150.jh1 vs. ExPASy Swiss-Prot
Match: Q9LSF8 (Dimethylnonatriene synthase OS=Arabidopsis thaliana OX=3702 GN=CYP82G1 PE=1 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 5.1e-04
Identity = 20/44 (45.45%), Postives = 30/44 (68.18%), Query Frame = 0


Query: 5   NFQSLPFGGGRRNCPGMNVGIVMMKLV----YQGFNIFRVSKMP 45
           NF+ +PFG GRR+CPG+N+G+ ++  V     QGF + +VS  P
Sbjct: 439 NFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSDEP 482

[Close]

BLAST of MELO3C034150.jh1 vs. ExPASy Swiss-Prot
Match: O81972 (Cytochrome P450 82A2 OS=Glycine max OX=3847 GN=CYP82A2 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 8.6e-04
Identity = 18/32 (56.25%), Postives = 24/32 (75.00%), Query Frame = 0


Query: 4   QNFQSLPFGGGRRNCPGMNVGIVMMKLVYQGF 36
           Q+FQ LPFGGGRR CPG+N+G+  ++L    F
Sbjct: 445 QHFQLLPFGGGRRICPGINLGLQTVRLTLASF 476

[Close]

BLAST of MELO3C034150.jh1 vs. ExPASy TrEMBL
Match: A0A6J1G094 (cytochrome P450 71B37-like OS=Cucurbita moschata OX=3662 GN=LOC111449540 PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 7.51e-04
Identity = 26/70 (37.14%), Postives = 39/70 (55.71%), Query Frame = 0


Query: 4   QNFQSLPFGGGRRNCPGMNVGIVMMKLVYQGFNIFRVSKMPRCCKNTSRR-----STNKQ 63
           QNF+ LPFG GRR CPGMN+GIV M+L      +    K+P   K+         + +K+
Sbjct: 432 QNFEYLPFGAGRRICPGMNMGIVTMELALANLLLLFDWKLPNGMKDVDMEEDAGLTVSKK 491

Query: 64  LAISVHPIRF 68
           L + + P+ +
Sbjct: 492 LPLKLIPVGY 501

[Close]

BLAST of MELO3C034150.jh1 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 46.2 bits (108), Expect = 3.6e-05
Identity = 20/44 (45.45%), Postives = 30/44 (68.18%), Query Frame = 0


Query: 5   NFQSLPFGGGRRNCPGMNVGIVMMKLV----YQGFNIFRVSKMP 45
           NF+ +PFG GRR+CPG+N+G+ ++  V     QGF + +VS  P
Sbjct: 439 NFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSDEP 482

[Close]

BLAST of MELO3C034150.jh1 vs. TAIR 10
Match: AT3G53300.1 (cytochrome P450, family 71, subfamily B, polypeptide 31 )

HSP 1 Score: 43.9 bits (102), Expect = 1.8e-04
Identity = 16/27 (59.26%), Postives = 23/27 (85.19%), Query Frame = 0


Query: 4   QNFQSLPFGGGRRNCPGMNVGIVMMKL 31
           Q+++ LPFG GRRNCPGM +GI +++L
Sbjct: 427 QHYELLPFGAGRRNCPGMTLGITILEL 453

[Close]

BLAST of MELO3C034150.jh1 vs. TAIR 10
Match: AT3G53305.1 (cytochrome P450, family 71, subfamily B, polypeptide 32 )

HSP 1 Score: 43.9 bits (102), Expect = 1.8e-04
Identity = 16/27 (59.26%), Postives = 24/27 (88.89%), Query Frame = 0


Query: 4   QNFQSLPFGGGRRNCPGMNVGIVMMKL 31
           Q+++ LPFG GRR+CPGMN+GI +++L
Sbjct: 260 QDYKLLPFGAGRRSCPGMNLGITILEL 286

[Close]

BLAST of MELO3C034150.jh1 vs. TAIR 10
Match: AT3G53290.1 (cytochrome P450, family 71, subfamily B, polypeptide 30 pseudogene )

HSP 1 Score: 43.5 bits (101), Expect = 2.3e-04
Identity = 16/27 (59.26%), Postives = 24/27 (88.89%), Query Frame = 0


Query: 4   QNFQSLPFGGGRRNCPGMNVGIVMMKL 31
           Q+F+ LPFG GRR+CPGM++GI +++L
Sbjct: 334 QHFELLPFGAGRRSCPGMSLGITILEL 360

[Close]

BLAST of MELO3C034150.jh1 vs. TAIR 10
Match: AT5G35715.1 (cytochrome P450, family 71, subfamily B, polypeptide 8 )

HSP 1 Score: 42.0 bits (97), Expect = 6.8e-04
Identity = 15/27 (55.56%), Postives = 23/27 (85.19%), Query Frame = 0


Query: 4   QNFQSLPFGGGRRNCPGMNVGIVMMKL 31
           Q+++ LPFG GRR+CPGM +GI +++L
Sbjct: 365 QHYELLPFGAGRRSCPGMTLGITILEL 391

The following BLAST results are available for this feature:

BLAST of MELO3C034150.jh1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_026432287.1	1.80e-04	43.08	cytochrome P450 71B20-like [Papaver somniferum]	[more]
XP_026432067.1	6.37e-04	37.33	cytochrome P450 71B34-like [Papaver somniferum]	[more]
XP_026383323.1	7.24e-04	70.00	cytochrome P450 71A1-like [Papaver somniferum]	[more]

BLAST of MELO3C034150.jh1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSF8	5.1e-04	45.45	Dimethylnonatriene synthase OS=Arabidopsis thaliana OX=3702 GN=CYP82G1 PE=1 SV=1	[more]
O81972	8.6e-04	56.25	Cytochrome P450 82A2 OS=Glycine max OX=3847 GN=CYP82A2 PE=2 SV=1	[more]

BLAST of MELO3C034150.jh1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G094	7.51e-04	37.14	cytochrome P450 71B37-like OS=Cucurbita moschata OX=3662 GN=LOC111449540 PE=3 SV...	[more]

BLAST of MELO3C034150.jh1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25180.1	3.6e-05	45.45	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]
AT3G53300.1	1.8e-04	59.26	cytochrome P450, family 71, subfamily B, polypeptide 31	[more]
AT3G53305.1	1.8e-04	59.26	cytochrome P450, family 71, subfamily B, polypeptide 32	[more]
AT3G53290.1	2.3e-04	59.26	cytochrome P450, family 71, subfamily B, polypeptide 30 pseudogene	[more]
AT5G35715.1	6.8e-04	55.56	cytochrome P450, family 71, subfamily B, polypeptide 8	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 4..32 e-value: 4.8E-7 score: 28.9
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 2..60 e-value: 3.9E-6 score: 28.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 4..32
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 11..20

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C034150.jh1.t1	MELO3C034150.jh1.t1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen