MELO3C032321 (gene) Melon (DHL92) v4

BLAST of MELO3C032321 vs. NCBI nr
Match: XP_008452823.1 (PREDICTED: uncharacterized transporter C405.03c-like [Cucumis melo])

HSP 1 Score: 713.4 bits (1840), Expect = 1.1e-201
Identity = 375/403 (93.05%), Postives = 376/403 (93.30%), Query Frame = 0

Query: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60
           MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV
Sbjct: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60

Query: 61  CSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREELG 120
           CSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREELG
Sbjct: 61  CSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREELG 120

Query: 121 QPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFA 180
           QPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFA
Sbjct: 121 QPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFA 180

Query: 181 LLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFGLL 240
           LLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFGLL
Sbjct: 181 LLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFGLL 240

Query: 241 SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYGQTEVFDHENLSTSLTSYDHETVLISKVL 300
           SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGY          L T L  +           
Sbjct: 241 SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGY--------VGLFTILGLW----------- 300

Query: 301 LAVWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSL 360
             VWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSL
Sbjct: 301 WLVWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSL 360

Query: 361 TIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIST 404
           TIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIST
Sbjct: 361 TIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIST 384

BLAST of MELO3C032321 vs. NCBI nr
Match: XP_004145580.1 (uncharacterized transporter C405.03c [Cucumis sativus])

HSP 1 Score: 684.5 bits (1765), Expect = 5.6e-193
Identity = 360/403 (89.33%), Postives = 367/403 (91.07%), Query Frame = 0

Query: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60
           MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV
Sbjct: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60

Query: 61  CSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREELG 120
           CSLLNPH+LLDNNY SIISTSIGIDGPLRFNE+HYNLD SMGHCLIINDKDLST   E G
Sbjct: 61  CSLLNPHSLLDNNYDSIISTSIGIDGPLRFNEIHYNLDESMGHCLIINDKDLSTTEREEG 120

Query: 121 QPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFA 180
           QPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFA
Sbjct: 121 QPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFA 180

Query: 181 LLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFGLL 240
           LLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISE RG SITGDIFGLL
Sbjct: 181 LLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISESRGLSITGDIFGLL 240

Query: 241 SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYGQTEVFDHENLSTSLTSYDHETVLISKVL 300
           SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGY          L T L  +           
Sbjct: 241 SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGY--------VGLFTVLGLW----------- 300

Query: 301 LAVWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSL 360
             VWPLTA+GIEPPLKFPPS S+TEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSL
Sbjct: 301 WLVWPLTAMGIEPPLKFPPSTSLTEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSL 360

Query: 361 TIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIST 404
           TIPLAMLADV+LHGRRYSALYILGCIQVFAGFLIVNFSDK+ST
Sbjct: 361 TIPLAMLADVILHGRRYSALYILGCIQVFAGFLIVNFSDKVST 384

BLAST of MELO3C032321 vs. NCBI nr
Match: XP_038899552.1 (uncharacterized vacuolar membrane protein YML018C-like [Benincasa hispida])

HSP 1 Score: 646.0 bits (1665), Expect = 2.2e-181
Identity = 342/403 (84.86%), Postives = 363/403 (90.07%), Query Frame = 0

Query: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60
           MGCKYK GLGLICTAVLIWVASAEITQRIFSEYKHPFA+SYLGVSLMVIYLP+AV+KDLV
Sbjct: 55  MGCKYKFGLGLICTAVLIWVASAEITQRIFSEYKHPFAISYLGVSLMVIYLPMAVVKDLV 114

Query: 61  CSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREELG 120
           CSL+NPHNLLDNN GS++S+SIG+DGPLRFN++HYN D SMGH LII DKDLS EREE G
Sbjct: 115 CSLINPHNLLDNN-GSVMSSSIGLDGPLRFNDVHYNADDSMGHSLIIRDKDLS-EREE-G 174

Query: 121 QPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFA 180
            PLIPNFE SHHKVSTWE+IKCS+YLTPLWF+TEYFSNSALANTSVATATILNSTSGLFA
Sbjct: 175 HPLIPNFE-SHHKVSTWEVIKCSIYLTPLWFSTEYFSNSALANTSVATATILNSTSGLFA 234

Query: 181 LLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFGLL 240
           LLFGAL GQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISE RG++ITGD+FGLL
Sbjct: 235 LLFGALFGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISESRGQTITGDVFGLL 294

Query: 241 SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYGQTEVFDHENLSTSLTSYDHETVLISKVL 300
           SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGY          L T L  +           
Sbjct: 295 SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGY--------VGLFTILGLW----------- 354

Query: 301 LAVWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSL 360
             VWPLTAVGIEPPLKFPPSKSI+EI+LLNGF+GSVLSDYFWALSVIWTSPLVATLGMSL
Sbjct: 355 WLVWPLTAVGIEPPLKFPPSKSISEILLLNGFIGSVLSDYFWALSVIWTSPLVATLGMSL 414

Query: 361 TIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIST 404
           TIPLAMLADVVLHGRRYSALYILGCIQVFAGF IVNFSDK+ST
Sbjct: 415 TIPLAMLADVVLHGRRYSALYILGCIQVFAGFFIVNFSDKLST 434

BLAST of MELO3C032321 vs. NCBI nr
Match: KGN55414.1 (hypothetical protein Csa_012018 [Cucumis sativus])

HSP 1 Score: 592.8 bits (1527), Expect = 2.2e-165
Identity = 314/357 (87.96%), Postives = 321/357 (89.92%), Query Frame = 0

Query: 47  MVIYLPVAVLKDLVCSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLI 106
           MVIYLPVAVLKDLVCSLLNPH+LLDNNY SIISTSIGIDGPLRFNE+HYNLD SMGHCLI
Sbjct: 1   MVIYLPVAVLKDLVCSLLNPHSLLDNNYDSIISTSIGIDGPLRFNEIHYNLDESMGHCLI 60

Query: 107 INDKDLSTEREELGQPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSV 166
           INDKDLST   E GQPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSV
Sbjct: 61  INDKDLSTTEREEGQPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSV 120

Query: 167 ATATILNSTSGLFALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISE 226
           ATATILNSTSGLFALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISE
Sbjct: 121 ATATILNSTSGLFALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISE 180

Query: 227 PRGRSITGDIFGLLSAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYGQTEVFDHENLSTSL 286
            RG SITGDIFGLLSAAIYGLFTVLLKKSAGSEGDKIDVQKFFGY          L T L
Sbjct: 181 SRGLSITGDIFGLLSAAIYGLFTVLLKKSAGSEGDKIDVQKFFGY--------VGLFTVL 240

Query: 287 TSYDHETVLISKVLLAVWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSV 346
             +             VWPLTA+GIEPPLKFPPS S+TEIVLLNGFVGSVLSDYFWALSV
Sbjct: 241 GLW-----------WLVWPLTAMGIEPPLKFPPSTSLTEIVLLNGFVGSVLSDYFWALSV 300

Query: 347 IWTSPLVATLGMSLTIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIST 404
           IWTSPLVATLGMSLTIPLAMLADV+LHGRRYSALYILGCIQVFAGFLIVNFSDK+ST
Sbjct: 301 IWTSPLVATLGMSLTIPLAMLADVILHGRRYSALYILGCIQVFAGFLIVNFSDKVST 338

BLAST of MELO3C032321 vs. NCBI nr
Match: XP_022982173.1 (thiamine-repressible mitochondrial transport protein THI74-like isoform X4 [Cucurbita maxima])

HSP 1 Score: 570.9 bits (1470), Expect = 9.0e-159
Identity = 315/404 (77.97%), Postives = 341/404 (84.41%), Query Frame = 0

Query: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60
           MG +YK GLGLIC AVLIWVASAEITQRIF+EYK PFALSYLGVSLMV+YLPVAV+KDLV
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 61  CSLLNPHNLLDN--NYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREE 120
            SLLNP  L D+  N GS+ S+SIG+D PL+FNE+HYN D  +  CL  +DKDLS EREE
Sbjct: 61  YSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYNFDEGLKSCL-TSDKDLS-EREE 120

Query: 121 LGQPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGL 180
            GQPL+ NFE SHHKVSTWEII+ SLYLTPLWFTTEYFSNSALANTSVATATILNSTSGL
Sbjct: 121 -GQPLMSNFE-SHHKVSTWEIIERSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGL 180

Query: 181 FALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFG 240
           FALLFGALVGQESITI+KVVAVFISMAGV+MTTLGKTWATN F +ISE RGR+ITGDIF 
Sbjct: 181 FALLFGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDIFC 240

Query: 241 LLSAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYGQTEVFDHENLSTSLTSYDHETVLISK 300
           LLSAAIYGLFTVLLKK AGS GDKIDVQKFFGY          L T           I  
Sbjct: 241 LLSAAIYGLFTVLLKKLAGSGGDKIDVQKFFGY--------VGLFT-----------IVG 300

Query: 301 VLLAVWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGM 360
           +   VWPLTA+GIEPP KFPPSKSITE V+LNGFVG+VLSDYFWALSVIWTSPLV+ LGM
Sbjct: 301 LWWLVWPLTAMGIEPPFKFPPSKSITESVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGM 360

Query: 361 SLTIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIS 403
           SLTIPLAMLAD+VLHGRRYS LYI+GC+QVFAGF IVNFSDKIS
Sbjct: 361 SLTIPLAMLADIVLHGRRYSTLYIVGCVQVFAGFFIVNFSDKIS 381

BLAST of MELO3C032321 vs. ExPASy Swiss-Prot
Match: Q03730 (Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YML018C PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 4.6e-33
Identity = 117/414 (28.26%), Postives = 197/414 (47.58%), Query Frame = 0

Query: 4   KYKIGLGLICTAVLIWVASAEITQRIFSE--YKHPFALSYLGVSLMVIYL-PVAVLKDLV 63
           ++ +GL ++   +++WV S+ +   IF +  Y+ PF ++Y   +  + YL P A  K +V
Sbjct: 12  RWTLGLLMLGLVIILWVLSSFLINLIFEDDSYRKPFFITYTNTAAFIFYLFPTA--KAVV 71

Query: 64  CSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREELG 123
                  N  D    ++              E+    +G+            S    ++ 
Sbjct: 72  V------NYKDTGRANV------------HRELIMEEEGTGSD---------SNRSVDMT 131

Query: 124 QPLIPNFESSHH-----KVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNST 183
            PL+ N E+  H     +++ +E IK S     LWFT    +N++LA TSVA+ TIL++T
Sbjct: 132 SPLLTNLEAGTHANQKKRLTLYETIKLSAEFCILWFTANLVTNASLAFTSVASQTILSTT 191

Query: 184 SGLFALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLII-----SEPRGR 243
           S  F L  GA+   ES++ SKV+  FIS  G+IM T   +    +  I           +
Sbjct: 192 SSFFTLFIGAICHVESLSKSKVLGSFISFVGIIMVTKSDSHQRYQRHIADVSGDDNDAVQ 251

Query: 244 SITGDIFGLLSAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYGQTEVFDHENLSTSLTSYD 303
            + G++  L  A +YG+++ LLK+  G E  +++++ FFG+    +F+            
Sbjct: 252 VLIGNLLALAGAVLYGVYSTLLKREVGDE-TRVNMKIFFGF--VGLFN------------ 311

Query: 304 HETVLISKVLLAVWP----LTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSV 363
                    LL +WP    L   G E P   P    +  I+ +N  + + +SD+ WA ++
Sbjct: 312 ---------LLFLWPSLIVLDFFGWE-PFSLPKDPKVVVIIFVNCLI-TFVSDFCWAKAM 370

Query: 364 IWTSPLVATLGMSLTIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDK 401
           + TSPL  T+G+S+TIPLAM  DV+   +  SALY+ G   +   F I+N S +
Sbjct: 372 LLTSPLTVTVGLSITIPLAMFGDVIFKHKTMSALYLFGATLILGSFFIINKSSE 370

BLAST of MELO3C032321 vs. ExPASy Swiss-Prot
Match: Q8R314 (Solute carrier family 35 member F5 OS=Mus musculus OX=10090 GN=Slc35f5 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.5e-31
Identity = 118/436 (27.06%), Postives = 200/436 (45.87%), Query Frame = 0

Query: 7   IGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYL--------------- 66
           +G+ ++    +IWVAS+E+T  +F++Y  PF  ++   S+ V+YL               
Sbjct: 71  LGIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYLLGFIIWKPWRQQCTR 130

Query: 67  -----PVAVLKD-----LVCS------------LLNPHNLLDNNYGSIISTSIGIDGPLR 126
                P A   D       C+            L  P    D     + ST+IG +   +
Sbjct: 131 GFRGKPAAFFADAEGYFAACTTDTSMSSSLSEPLYVPVKFHDLPSEKLESTNIGTEKTPK 190

Query: 127 FNEMHYNLDGSMGHCLIINDKDLSTEREELGQPLIPNFES---SHHKVSTWEIIKCSLYL 186
            + + ++    M    + +   L  +   +  P + + ES   +  K++  ++ K S + 
Sbjct: 191 KSRVRFS--NIMEIRQLPSSHALEAKLSRMSYPTVKDQESILKTVGKLTATQVAKISFFF 250

Query: 187 TPLWFTTEYFSNSALANTSVATATILNSTSGLFALLFGALVGQES---ITISKVVAVFIS 246
             +WF        AL++T VA   IL+STSGLF L+  A+    S    T+SK++AV +S
Sbjct: 251 CFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSGDRFTLSKLLAVILS 310

Query: 247 MAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFGLLSAAIYGLFTVLLKKSAGSEGDKI 306
           + GV++  L  +          +  GR   G I+ L  A  Y ++ V++K+    E DK+
Sbjct: 311 IGGVVLVNLSGS---------EKSAGRDTIGSIWSLAGAMFYAVYIVMIKRKVDRE-DKL 370

Query: 307 DVQKFFGYGQTEVFDHENLSTSLTSYDHETVLISKVLLAVWP----LTAVGIEPPLKFPP 366
           D+  FFG+    +F+                     LL +WP    L   G E   +F P
Sbjct: 371 DIPMFFGF--VGLFN---------------------LLLLWPGFFLLHYTGFE-DFEF-P 430

Query: 367 SKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVVLHGRRYSA 396
           +K +   +++NG +G+VLS++ W      TS L+ TL +SLTIPL+++AD+ +   ++S 
Sbjct: 431 NKVVLLCIIINGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSW 469

BLAST of MELO3C032321 vs. ExPASy Swiss-Prot
Match: Q8WV83 (Solute carrier family 35 member F5 OS=Homo sapiens OX=9606 GN=SLC35F5 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 5.7e-31
Identity = 118/438 (26.94%), Postives = 198/438 (45.21%), Query Frame = 0

Query: 7   IGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVL-----KDLVC 66
           +G+ ++    +IWVAS+E+T  +F++Y  PF  ++   S+ V+YL   ++     +    
Sbjct: 71  LGIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYLLGFIIWKPWRQQCTR 130

Query: 67  SLLNPHNLL-------------DNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIIN 126
            L   H                D    S +S  + +  P++F+++      S     I  
Sbjct: 131 GLRGKHAAFFADAEGYFAACTTDTTMNSSLSEPLYV--PVKFHDLPSEKPESTN---IDT 190

Query: 127 DKDLSTEREELGQPL-IPNFESSH-----------------------HKVSTWEIIKCSL 186
           +K     R      + I    SSH                        K++  ++ K S 
Sbjct: 191 EKTPKKSRVRFSNIMEIRQLPSSHALEAKLSRMSYPVKEQESILKTVGKLTATQVAKISF 250

Query: 187 YLTPLWFTTEYFSNSALANTSVATATILNSTSGLFALLFGALVGQES---ITISKVVAVF 246
           +   +WF        AL++T VA   IL+STSGLF L+  A+    S    T+SK++AV 
Sbjct: 251 FFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSGDRFTLSKLLAVI 310

Query: 247 ISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFGLLSAAIYGLFTVLLKKSAGSEGD 306
           +S+ GV++  L  +          +P GR   G I+ L  A +Y ++ V++K+    E D
Sbjct: 311 LSIGGVVLVNLAGS---------EKPAGRDTVGSIWSLAGAMLYAVYIVMIKRKVDRE-D 370

Query: 307 KIDVQKFFGYGQTEVFDHENLSTSLTSYDHETVLISKVLLAVWP----LTAVGIEPPLKF 366
           K+D+  FFG+    +F+                     LL +WP    L   G E   +F
Sbjct: 371 KLDIPMFFGF--VGLFN---------------------LLLLWPGFFLLHYTGFE-DFEF 430

Query: 367 PPSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVVLHGRRY 396
            P+K +   +++NG +G+VLS++ W      TS L+ TL +SLTIPL+++AD+ +   ++
Sbjct: 431 -PNKVVLMCIIINGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQF 468

BLAST of MELO3C032321 vs. ExPASy Swiss-Prot
Match: A6QL92 (Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.3e-30
Identity = 114/435 (26.21%), Postives = 203/435 (46.67%), Query Frame = 0

Query: 7   IGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVL-----KDLVC 66
           +G+ ++    +IWVAS+E+T  +F++Y  PF  ++   S+ V+YL   ++     +    
Sbjct: 71  LGIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYLLGFIVWKPWRQQCTR 130

Query: 67  SLLNPHNLL-------------DNNYGSIISTSIGIDGPLRFNEM------HYNLD---- 126
                H                D    S +S  + +  P++F+++      + N+D    
Sbjct: 131 GFRGKHATFFADAEGYFAACTTDTTMNSSLSEPLYV--PVKFHDLPSEKPENTNIDTEKI 190

Query: 127 ---------GSMGHCLIINDKDLSTEREELGQPLIPNFES---SHHKVSTWEIIKCSLYL 186
                      M    + +   L  +   +  P +   ES   +  K++  ++ K S + 
Sbjct: 191 PKKSRVRFSNIMEIRQLPSSHALEAKLSRMSYPTVKEQESLLKTVGKLTATQVAKISFFF 250

Query: 187 TPLWFTTEYFSNSALANTSVATATILNSTSGLFALLFGALVGQES---ITISKVVAVFIS 246
             +WF   +    AL++T VA   IL+STSGLF L+  A+    S    T+SK++AV +S
Sbjct: 251 CFVWFLANFSYQEALSDTQVAIVNILSSTSGLFTLILAAMFPSNSGDRFTLSKLLAVILS 310

Query: 247 MAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFGLLSAAIYGLFTVLLKKSAGSEGDKI 306
           + GV++  L  +          +  GR+  G I+ L+ A +Y ++ V++K+    E DK+
Sbjct: 311 IGGVVLVNLSGS---------EKSPGRNTIGSIWSLVGAMLYAVYIVMIKRKVDRE-DKL 370

Query: 307 DVQKFFGYGQTEVFDHENLSTSLTSYDHETVLISKVLLAVWP----LTAVGIEPPLKFPP 366
           D+  FFG+    +F+                     LL +WP    L   G E   +F P
Sbjct: 371 DIPMFFGF--VGLFN---------------------LLLLWPGFFLLHYTGFE-DFEF-P 430

Query: 367 SKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVVLHGRRYSA 395
           +K +   +++NG +G+VLS++ W      TS L+ TL +SLTIPL+++AD+ +   ++S 
Sbjct: 431 NKVVLMCIVINGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSW 468

BLAST of MELO3C032321 vs. ExPASy Swiss-Prot
Match: O94654 (Uncharacterized transporter C405.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC405.03c PE=3 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 6.3e-30
Identity = 111/407 (27.27%), Postives = 190/407 (46.68%), Query Frame = 0

Query: 4   KYKIGLGLICTAVLIWVASAEITQRIFSE--YKHPFALSYLGVSLMVIYL---PVAVLKD 63
           K+ +G+ L+   V +W+ S+ +T  +  +  +  PF ++Y+     V YL     +  K 
Sbjct: 8   KHALGVVLLLFVVFLWLISSFLTSSLLDDDNFFSPFLITYINTGTFVFYLIPWYFSEKKT 67

Query: 64  LVCSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREE 123
               L++  ++ ++ + S  +     + PL F +  Y    S+G C+I            
Sbjct: 68  RKHRLMSELSMYESVHDSSFNLGTRPNSPLGFRQTAYL---SLGFCII------------ 127

Query: 124 LGQPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGL 183
                                          WF   YFSNS+L  T+VA+ TI++S SG 
Sbjct: 128 -------------------------------WFAANYFSNSSLGFTNVASFTIISSMSGF 187

Query: 184 FALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFG 243
           F L  G +V  E  T+SK++A+  S+ GVI+     T    +  +   P  R   G+ + 
Sbjct: 188 FTLGLGTIVNVERFTLSKLLALMASVGGVIIVV---TQDAKQADLNDSPPSRPALGNAYA 247

Query: 244 LLSAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYGQTEVFDHENLSTSLTSYDHETVLISK 303
           LL+A +YG ++V++K    +E   +  + FFG     +FD                    
Sbjct: 248 LLAALLYGCYSVMVKFHI-TEESCVSTRLFFGL--VGLFD-------------------- 307

Query: 304 VLLAVWPLTAV----GIEPPLKFP-PSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLV 363
            L+ +WP   +    G+E   +F  PS +   IVL+     + +SDY W ++++ TSPL+
Sbjct: 308 -LILLWPFLIILHLYGVE---RFSLPSTTAGLIVLIINASITFVSDYLWVIAMLMTSPLL 338

Query: 364 ATLGMSLTIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDK 401
            T+GMSL+IPLA+  D++L G   +   ILG + VFAGF++VN++ +
Sbjct: 368 VTVGMSLSIPLALFFDILLKGHYLNFSLILGSLLVFAGFIVVNYNQQ 338

BLAST of MELO3C032321 vs. ExPASy TrEMBL
Match: A0A1S3BUT3 (uncharacterized transporter C405.03c-like OS=Cucumis melo OX=3656 GN=LOC103493733 PE=3 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 5.4e-202
Identity = 375/403 (93.05%), Postives = 376/403 (93.30%), Query Frame = 0

Query: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60
           MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV
Sbjct: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60

Query: 61  CSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREELG 120
           CSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREELG
Sbjct: 61  CSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREELG 120

Query: 121 QPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFA 180
           QPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFA
Sbjct: 121 QPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFA 180

Query: 181 LLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFGLL 240
           LLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFGLL
Sbjct: 181 LLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFGLL 240

Query: 241 SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYGQTEVFDHENLSTSLTSYDHETVLISKVL 300
           SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGY          L T L  +           
Sbjct: 241 SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGY--------VGLFTILGLW----------- 300

Query: 301 LAVWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSL 360
             VWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSL
Sbjct: 301 WLVWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSL 360

Query: 361 TIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIST 404
           TIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIST
Sbjct: 361 TIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIST 384

BLAST of MELO3C032321 vs. ExPASy TrEMBL
Match: A0A0A0L306 (EamA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G651810 PE=3 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 1.1e-165
Identity = 314/357 (87.96%), Postives = 321/357 (89.92%), Query Frame = 0

Query: 47  MVIYLPVAVLKDLVCSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLI 106
           MVIYLPVAVLKDLVCSLLNPH+LLDNNY SIISTSIGIDGPLRFNE+HYNLD SMGHCLI
Sbjct: 1   MVIYLPVAVLKDLVCSLLNPHSLLDNNYDSIISTSIGIDGPLRFNEIHYNLDESMGHCLI 60

Query: 107 INDKDLSTEREELGQPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSV 166
           INDKDLST   E GQPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSV
Sbjct: 61  INDKDLSTTEREEGQPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSV 120

Query: 167 ATATILNSTSGLFALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISE 226
           ATATILNSTSGLFALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISE
Sbjct: 121 ATATILNSTSGLFALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISE 180

Query: 227 PRGRSITGDIFGLLSAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYGQTEVFDHENLSTSL 286
            RG SITGDIFGLLSAAIYGLFTVLLKKSAGSEGDKIDVQKFFGY          L T L
Sbjct: 181 SRGLSITGDIFGLLSAAIYGLFTVLLKKSAGSEGDKIDVQKFFGY--------VGLFTVL 240

Query: 287 TSYDHETVLISKVLLAVWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSV 346
             +             VWPLTA+GIEPPLKFPPS S+TEIVLLNGFVGSVLSDYFWALSV
Sbjct: 241 GLW-----------WLVWPLTAMGIEPPLKFPPSTSLTEIVLLNGFVGSVLSDYFWALSV 300

Query: 347 IWTSPLVATLGMSLTIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIST 404
           IWTSPLVATLGMSLTIPLAMLADV+LHGRRYSALYILGCIQVFAGFLIVNFSDK+ST
Sbjct: 301 IWTSPLVATLGMSLTIPLAMLADVILHGRRYSALYILGCIQVFAGFLIVNFSDKVST 338

BLAST of MELO3C032321 vs. ExPASy TrEMBL
Match: A0A6J1J463 (thiamine-repressible mitochondrial transport protein THI74-like isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111481080 PE=3 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 4.3e-159
Identity = 315/404 (77.97%), Postives = 341/404 (84.41%), Query Frame = 0

Query: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60
           MG +YK GLGLIC AVLIWVASAEITQRIF+EYK PFALSYLGVSLMV+YLPVAV+KDLV
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 61  CSLLNPHNLLDN--NYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREE 120
            SLLNP  L D+  N GS+ S+SIG+D PL+FNE+HYN D  +  CL  +DKDLS EREE
Sbjct: 61  YSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYNFDEGLKSCL-TSDKDLS-EREE 120

Query: 121 LGQPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGL 180
            GQPL+ NFE SHHKVSTWEII+ SLYLTPLWFTTEYFSNSALANTSVATATILNSTSGL
Sbjct: 121 -GQPLMSNFE-SHHKVSTWEIIERSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGL 180

Query: 181 FALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFG 240
           FALLFGALVGQESITI+KVVAVFISMAGV+MTTLGKTWATN F +ISE RGR+ITGDIF 
Sbjct: 181 FALLFGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDIFC 240

Query: 241 LLSAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYGQTEVFDHENLSTSLTSYDHETVLISK 300
           LLSAAIYGLFTVLLKK AGS GDKIDVQKFFGY          L T           I  
Sbjct: 241 LLSAAIYGLFTVLLKKLAGSGGDKIDVQKFFGY--------VGLFT-----------IVG 300

Query: 301 VLLAVWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGM 360
           +   VWPLTA+GIEPP KFPPSKSITE V+LNGFVG+VLSDYFWALSVIWTSPLV+ LGM
Sbjct: 301 LWWLVWPLTAMGIEPPFKFPPSKSITESVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGM 360

Query: 361 SLTIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIS 403
           SLTIPLAMLAD+VLHGRRYS LYI+GC+QVFAGF IVNFSDKIS
Sbjct: 361 SLTIPLAMLADIVLHGRRYSTLYIVGCVQVFAGFFIVNFSDKIS 381

BLAST of MELO3C032321 vs. ExPASy TrEMBL
Match: A0A6J1CLJ1 (uncharacterized vacuolar membrane protein YML018C-like OS=Momordica charantia OX=3673 GN=LOC111012569 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 3.1e-157
Identity = 311/404 (76.98%), Postives = 339/404 (83.91%), Query Frame = 0

Query: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60
           MG +Y+IGLGLI TAV+IWV SAEITQRIFSEYKHPFALSYLGVSLMV+YLPVAV++DL+
Sbjct: 1   MGWRYRIGLGLILTAVVIWVTSAEITQRIFSEYKHPFALSYLGVSLMVVYLPVAVVRDLI 60

Query: 61  CSLLNPH--NLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREE 120
            SLLNP   N   +N GS++S+SIG+D PLRFNE H +LD  MG CL I+DKDLS EREE
Sbjct: 61  FSLLNPDLPNNHPDNGGSVLSSSIGLDFPLRFNEAHNSLDEGMGSCL-ISDKDLS-EREE 120

Query: 121 LGQPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGL 180
            GQPLIP  E   ++VS+WEI KCSL+LTPLWFTTEYFSNSALANTSVATATILNSTSGL
Sbjct: 121 -GQPLIPKIEPK-NEVSSWEIAKCSLFLTPLWFTTEYFSNSALANTSVATATILNSTSGL 180

Query: 181 FALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFG 240
           FALLFGA++GQESITISKVVAV ISMAGV MTTLGKTWATNE+LIISE RGR++ GD+FG
Sbjct: 181 FALLFGAVLGQESITISKVVAVLISMAGVAMTTLGKTWATNEYLIISESRGRTVMGDMFG 240

Query: 241 LLSAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYGQTEVFDHENLSTSLTSYDHETVLISK 300
           LLSA  YGLFTVLLKKSAGSEGDKIDVQKFFGY          L T L  +         
Sbjct: 241 LLSAVTYGLFTVLLKKSAGSEGDKIDVQKFFGY--------VGLFTILGLW--------- 300

Query: 301 VLLAVWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGM 360
               VWPLTAVGIEPPLKFP SKS+TEIVLLNGFVGSV+SDY WA SVIWTSPLVATLGM
Sbjct: 301 --WLVWPLTAVGIEPPLKFPASKSVTEIVLLNGFVGSVVSDYLWAHSVIWTSPLVATLGM 360

Query: 361 SLTIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIS 403
           SLTIPLAMLADVVLHGRRYSALYILGCIQVFAGF +VN SDK S
Sbjct: 361 SLTIPLAMLADVVLHGRRYSALYILGCIQVFAGFFLVNISDKFS 381

BLAST of MELO3C032321 vs. ExPASy TrEMBL
Match: A0A6J1FNF4 (thiamine-repressible mitochondrial transport protein THI74-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111445844 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.5e-154
Identity = 307/404 (75.99%), Postives = 335/404 (82.92%), Query Frame = 0

Query: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60
           MG +YK GLGLIC AVLIWVASAEITQRIF+EYK PFALSYLGVSLMV+YLPVAV+KDLV
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 61  CSLLNPHNLLDN--NYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREE 120
            SLLNP  L D+  N GS+ S+SIG+  PL+FNE+HYN D  +   L  +DKDLS E E 
Sbjct: 61  YSLLNPDLLNDHHENDGSVPSSSIGLGVPLKFNEIHYNFDEDLKSSL-TSDKDLSEEEE- 120

Query: 121 LGQPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGL 180
            GQPL+ NFE SHHKVST EII+ SLYLTP+WFTTEYFSNSALANTSVATATILNSTSGL
Sbjct: 121 -GQPLMSNFE-SHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGL 180

Query: 181 FALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFG 240
           FALLFGALVGQESITI+KVVAVFISMAGV MTTLGKTWATN F +I E RGR+ITGD+F 
Sbjct: 181 FALLFGALVGQESITITKVVAVFISMAGVAMTTLGKTWATNGFPVIIESRGRTITGDVFC 240

Query: 241 LLSAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYGQTEVFDHENLSTSLTSYDHETVLISK 300
           LLSAAIYGLFTVLLKK AGS GDKIDVQKFFGY          L T           I  
Sbjct: 241 LLSAAIYGLFTVLLKKLAGSGGDKIDVQKFFGY--------VGLFT-----------IVG 300

Query: 301 VLLAVWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGM 360
           +   VWPLTA+GIEPP KFPPSKSITEIV+LNGFVG+VLSDYFWALSVIWTSPLV+ LGM
Sbjct: 301 LWWLVWPLTAMGIEPPFKFPPSKSITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGM 360

Query: 361 SLTIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIS 403
           SLTIPLAMLAD+VLHGRRYS +YI+GC+QVFAGF IVNFSDKIS
Sbjct: 361 SLTIPLAMLADMVLHGRRYSTIYIVGCVQVFAGFFIVNFSDKIS 381

BLAST of MELO3C032321 vs. TAIR 10
Match: AT4G32140.1 (EamA-like transporter family )

HSP 1 Score: 387.1 bits (993), Expect = 1.7e-107
Identity = 217/412 (52.67%), Postives = 277/412 (67.23%), Query Frame = 0

Query: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60
           MG +YK GL LI T V+IWV SAE+TQ IF+ YK PFA++YLG SLM++YLPVA LKD +
Sbjct: 1   MGWRYKAGLFLIGTVVIIWVTSAEVTQDIFTAYKQPFAVTYLGASLMIVYLPVAFLKDWL 60

Query: 61  CSLLNPHNLLDNNYGSII-STSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREEL 120
           C  L+  +   N   ++   +S+ +  PLR   +   L G++      +++ LS+  EE 
Sbjct: 61  CRYLDRRSSKSNKIPALTDDSSVELGSPLRHKIIEMGLQGTI--TKKDSEEHLSSHEEED 120

Query: 121 GQPLIPNFE------SSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILN 180
            +PLI              +++T +I    LYL P+WF TEY SN+ALA TSVA+ T+L+
Sbjct: 121 ERPLIGKVREEVQTLKQRKEITTKQIAMYGLYLAPIWFVTEYLSNAALARTSVASTTVLS 180

Query: 181 STSGLFALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRG-RSI 240
           STSGLF L  G  +GQ+++ +SKVVAVF+SMAGV+MTTLGKTWA++E  + S   G RS+
Sbjct: 181 STSGLFTLFIGVFLGQDTLNLSKVVAVFVSMAGVVMTTLGKTWASDESQLNSSLNGERSL 240

Query: 241 TGDIFGLLSAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYGQTEVFDHENLSTSLTSYDHE 300
            GD+FGLLSA  YGLFTVLLKK AG EG+ +DVQK FGY                     
Sbjct: 241 MGDLFGLLSAVSYGLFTVLLKKFAGEEGEGVDVQKLFGY--------------------- 300

Query: 301 TVLISKVLL--AVWPLTAVGIEPPLKFPPSKSITEIVLLNGFVGSVLSDYFWALSVIWTS 360
             L + V L   VWPLTA+GIEP    P S  + E+VL NGF+GSVLSDYFWALSV+WT+
Sbjct: 301 IGLFTLVALWWLVWPLTALGIEPKFTIPHSVKVDEVVLANGFIGSVLSDYFWALSVVWTT 360

Query: 361 PLVATLGMSLTIPLAMLADVVLHGRRYSALYILGCIQVFAGFLIVNFSDKIS 403
           PLVATLGMSLTIPLAM+AD+++HGR YSA+YILG  QVFAGF+I N SD  S
Sbjct: 361 PLVATLGMSLTIPLAMVADMMIHGRHYSAIYILGSTQVFAGFVIANISDLFS 389

BLAST of MELO3C032321 vs. TAIR 10
Match: AT3G07080.1 (EamA-like transporter family )

HSP 1 Score: 96.7 bits (239), Expect = 4.6e-20
Identity = 102/428 (23.83%), Postives = 183/428 (42.76%), Query Frame = 0

Query: 4   KYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPV----AVLKDL 63
           ++ +GL  +     IW+A++ + Q +      PF ++++  SL V+YLP+      L+D 
Sbjct: 7   RWVLGLIYLFAVATIWIAASFVVQSVVEAGVSPFLITFICNSLFVVYLPLFEIGRYLEDA 66

Query: 64  VCSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLS------ 123
             SLL   +   +    + S    + G    +      D +    L++ ++++S      
Sbjct: 67  YGSLLFWRSKRSHLMELVESEKAVLLGK---DVSGVKSDATESSGLVVREQEISEGGNGI 126

Query: 124 ---TEREELGQPLIPNFESSHHKVST--------W---EIIKCSLYLTPLWFTTEYFSNS 183
               E  EL      N       VS         W    + K SL + P WF  +   N 
Sbjct: 127 ESGLENIELEIDASVNVSDGASGVSNKGLDEKGRWTRMRVAKVSLVICPFWFLAQLTFNV 186

Query: 184 ALANTSVATATILNSTSGLFALLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATN 243
           +L  T+V + TIL+S S LF  L   +   E  T  K+ +V + M+G I+ ++G + + +
Sbjct: 187 SLKYTTVTSNTILSSASSLFTFLVSLIFLGEKFTWLKLFSVLLCMSGTIIVSMGDSESKS 246

Query: 244 EFLIISEPRGRSITGDIFGLLSAAIYGLFTVLLKKSAGSEGD---KIDVQKFFGY---GQ 303
              +   P    + GDI  L+SAA+Y ++  L++K    + +   ++ + +F G+     
Sbjct: 247 N-AVAKNP----LLGDILSLVSAALYAVYITLIRKKLPDDDERNGRVSMAQFLGFLGLFN 306

Query: 304 TEVFDHENLSTSLTSYDHETVLISKVLLAVWPLTAVGIEPPLKFPPSKSITEIVLLNGFV 363
             +F    L  + T  +    L  K                           +V+  G +
Sbjct: 307 FFIFLPPALILNFTKRERFNALTLKQF------------------------GLVVGKGLL 366

Query: 364 GSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVVLHGRRYSALYILGCIQVFAGFL 402
            +VLSDY WA +V+ T+  VAT G+++ +PLA + D  L G + S    +G   V  GF 
Sbjct: 367 DNVLSDYLWAKAVLLTTTTVATAGLTIQVPLAAIVD-SLSGNKPSFTDYIGAAAVMVGFA 401