MELO3C031619.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C031619.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
DescriptionPectinesterase
Locationchr06: 5694851 .. 5697350 (-)
RNA-Seq ExpressionMELO3C031619.jh1
SyntenyMELO3C031619.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSstart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCATTCACCAGTAATCCATCTCTTCCGTCTCTAAATTCATGGCTGATTCATCCTCGAATTCCCTATTCATCAAACCGATTTACATCGCATTTGTGATCGCTTTTGCTTTCTTAATTTCATCTCCCATCGGTTCTTATGCCTTTACTGCACAAAATGTGATTGATTCGCCGTTGTTGACGAAGAAGATCGGTACAAATCGAACTATTAAGGTCGATATCAATGGAAATGGCGAATTCAAGTCCATTCAAGCTGCAGTCGATTCTGTTCCGGAAGGGAATTCGCAGTGGATGATCATCCATGTTAGGAAAGGAATTTATAGGTGATTTCTACGGTTTTCTTTTGCAGTTTTATTTTAGGATGTTGTTGATTTGATGAAATTGTTCATTTTTGTGGTCTGAATTTACGAAAACGAGAGCGTACGTCCTTATTTTTAATTTGGCTCTGTAGAGAGAAGGTTCATATTCCGTCGAGTAAGCCGTACATCTTCTTGAGAGGGAATGGAAAAGGAAGGACGTCAATCGTTTGGTCACAGAGCTCTAGTGATAACGTTGAATCTGCTACTTTTAAAGTTGAAGCTCAACATTTCATTGCTTTTGGCATTAGCTTCAAGGTCCGAATATTTCTCAGTTCTTTACAGTGATTCTCTCTCTCTCTCTCTCTCTCTCCCTTGCTTGAATTAGCTTCAAAACGTATTCTGTTAATGCATTGATTGCTTTTTCTGTTGGAAAATTTTGCTATTACAGAATGAGGCTCCGACTGGTGTGGCTTACACATCGCAAAATCAATCGGTAGCGGCATTCGTTGCGGCAGATAAAGCTGCATTTTACCACTGTGGATTCTATAGCACTCACAATACTCTGTTCGATTATAAAGGCAGACATTACTACGACAGTTGCTACATTCAAGGCTCCATTGATTTCATCTTTGGCCGAGGCAAATCCGTATTCCATGTAAAACTATTTTCCTCCCCTTTCTCCACCGTTGCTTGCTTACTGAAAAAAATTACAATGCGTTCTATTACATGCCTAGAATATTTATGACAAGCTCGTCTACCTCCATTCCATAACTATTTAGTTTTCATTTTTTAAATTAAAATTTTAAATATAGATTCCATGGATTTGATTTCTTAGTTTTAAACTTGAGTATCAGAAAATCATCCATATAAATGCTACTTTTATTAGTTTCTTAATCTTCTTAATGATGAAAATTCTAAGCCAAATTTGTAAAAGTAGCAGTACATTATGAGGAAACGGTGAGAAAACAAGATTTTAGAAAATGAAACTGTTATCAAACTTTCACTACATAATTATATGATCTTGTTATTGTCTATTGATTGATTTGTTATCTTATGAATCAAACTTCAATTTATTTGCTACCCAAAGTCAATAGGTTCAGTTATACATTTTGCTTTAGTTAAAAAGATTAGCAATGAAACATGAATGCATCATCATGGGATTTAAAAGAAGCTAGGCATTTGGAAAAGAGTTAAAAGAAATCTTCTATTTACTTTACATATATTACATTGAATGGAAAGAAATTAAAAAAAAAAAAAAAAAGATCTAGTGAGTTAAGAAGAGGTCAAAAAAAGAATCGAAAGATTGAGTACATGTTATACCAGCTAATTTCATTATATATATAATTGAGTAGATTTAATTTTTTTTCATGATATGTTCCATAAACAAGACAAGCATTAAAATCATACGTTGTCATTATTATAATTGGGTGTGTTACTGTATGGATGCAGAATTGTGAGATGTTTGTGATTGATGACAAAAGATTGACGATCCGTGGTTCAATCACAGCTCAAAACAGAAAGAGTGCAAATGAGAACAGTGGATTTGTTTTCATCAAAGGAAAGGTTTACGGAGTTGGCAGTACGTATTTGGGCAGAGCAAAAGGTGCACATTCTAGAGTCGTTTTTGCTAAAACTTACCTCTCCATCACCGTTGTTCCTGCTGGTTGGACCAATTGGAGCTACGTTGGCAGCACTGAGTAAGTGAAATGCACCTGAATATACTTTTTTCTTAAATATTTGAGGGACGTTGGATGGAGTTAGTAGTGTAGAACTTAAGAAATCCTAATTTTGTTATATATTACAGGAATCTGTACCATGCAGAATATGATTGTCGTGGACCAGGATCAGAATCAAAAAGTCGTGCACCATGGGCTAAACAGTTAACTAAAGAGGAAGTTGCCCCATTAATGGACGTTAGTTTTATTGATGGCACCGATTGGCTTCCTGCCTGGCTATGAAAACAACAACAATTAAATAGCTAAGCTAATATTACACACACTCTCCTTCATGCTAGCCCTCCTTCCCTTTTAAATTTCCATTACTCTACGGTCCTTTTTGGTATAATTATATTGTTTTTGTTTTTGTTTTTGCTTTTTTTGTTCTTTTTTTGTTTGTGACAATATCTTCATGCAAACTGCATTGTAAAGATATTCTTATTTAAAAATATACTTGATCGATGAGATGAAATACTAAAGAATTCCCTT

mRNA sequence

TTCATTCACCAGTAATCCATCTCTTCCGTCTCTAAATTCATGGCTGATTCATCCTCGAATTCCCTATTCATCAAACCGATTTACATCGCATTTGTGATCGCTTTTGCTTTCTTAATTTCATCTCCCATCGGTTCTTATGCCTTTACTGCACAAAATGTGATTGATTCGCCGTTGTTGACGAAGAAGATCGGTACAAATCGAACTATTAAGGTCGATATCAATGGAAATGGCGAATTCAAGTCCATTCAAGCTGCAGTCGATTCTGTTCCGGAAGGGAATTCGCAGTGGATGATCATCCATGTTAGGAAAGGAATTTATAGAGAGAAGGTTCATATTCCGTCGAGTAAGCCGTACATCTTCTTGAGAGGGAATGGAAAAGGAAGGACGTCAATCGTTTGGTCACAGAGCTCTAGTGATAACGTTGAATCTGCTACTTTTAAAGTTGAAGCTCAACATTTCATTGCTTTTGGCATTAGCTTCAAGAATGAGGCTCCGACTGGTGTGGCTTACACATCGCAAAATCAATCGGTAGCGGCATTCGTTGCGGCAGATAAAGCTGCATTTTACCACTGTGGATTCTATAGCACTCACAATACTCTGTTCGATTATAAAGGCAGACATTACTACGACAGTTGCTACATTCAAGGCTCCATTGATTTCATCTTTGGCCGAGGCAAATCCGTATTCCATAATTGTGAGATGTTTGTGATTGATGACAAAAGATTGACGATCCGTGGTTCAATCACAGCTCAAAACAGAAAGAGTGCAAATGAGAACAGTGGATTTGTTTTCATCAAAGGAAAGGTTTACGGAGTTGGCAGTACGTATTTGGGCAGAGCAAAAGGTGCACATTCTAGAGTCGTTTTTGCTAAAACTTACCTCTCCATCACCGTTGTTCCTGCTGGTTGGACCAATTGGAGCTACGTTGGCAGCACTGAGAATCTGTACCATGCAGAATATGATTGTCGTGGACCAGGATCAGAATCAAAAAGTCGTGCACCATGGGCTAAACAGTTAACTAAAGAGGAAGTTGCCCCATTAATGGACGTTAGTTTTATTGATGGCACCGATTGGCTTCCTGCCTGGCTATGAAAACAACAACAATTAAATAGCTAAGCTAATATTACACACACTCTCCTTCATGCTAGCCCTCCTTCCCTTTTAAATTTCCATTACTCTACGGTCCTTTTTGGTATAATTATATTGTTTTTGTTTTTGTTTTTGCTTTTTTTGTTCTTTTTTTGTTTGTGACAATATCTTCATGCAAACTGCATTGTAAAGATATTCTTATTTAAAAATATACTTGATCGATGAGATGAAATACTAAAGAATTCCCTT

Coding sequence (CDS)

ATGGCTGATTCATCCTCGAATTCCCTATTCATCAAACCGATTTACATCGCATTTGTGATCGCTTTTGCTTTCTTAATTTCATCTCCCATCGGTTCTTATGCCTTTACTGCACAAAATGTGATTGATTCGCCGTTGTTGACGAAGAAGATCGGTACAAATCGAACTATTAAGGTCGATATCAATGGAAATGGCGAATTCAAGTCCATTCAAGCTGCAGTCGATTCTGTTCCGGAAGGGAATTCGCAGTGGATGATCATCCATGTTAGGAAAGGAATTTATAGAGAGAAGGTTCATATTCCGTCGAGTAAGCCGTACATCTTCTTGAGAGGGAATGGAAAAGGAAGGACGTCAATCGTTTGGTCACAGAGCTCTAGTGATAACGTTGAATCTGCTACTTTTAAAGTTGAAGCTCAACATTTCATTGCTTTTGGCATTAGCTTCAAGAATGAGGCTCCGACTGGTGTGGCTTACACATCGCAAAATCAATCGGTAGCGGCATTCGTTGCGGCAGATAAAGCTGCATTTTACCACTGTGGATTCTATAGCACTCACAATACTCTGTTCGATTATAAAGGCAGACATTACTACGACAGTTGCTACATTCAAGGCTCCATTGATTTCATCTTTGGCCGAGGCAAATCCGTATTCCATAATTGTGAGATGTTTGTGATTGATGACAAAAGATTGACGATCCGTGGTTCAATCACAGCTCAAAACAGAAAGAGTGCAAATGAGAACAGTGGATTTGTTTTCATCAAAGGAAAGGTTTACGGAGTTGGCAGTACGTATTTGGGCAGAGCAAAAGGTGCACATTCTAGAGTCGTTTTTGCTAAAACTTACCTCTCCATCACCGTTGTTCCTGCTGGTTGGACCAATTGGAGCTACGTTGGCAGCACTGAGAATCTGTACCATGCAGAATATGATTGTCGTGGACCAGGATCAGAATCAAAAAGTCGTGCACCATGGGCTAAACAGTTAACTAAAGAGGAAGTTGCCCCATTAATGGACGTTAGTTTTATTGATGGCACCGATTGGCTTCCTGCCTGGCTATGA

Protein sequence

MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDINGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVWSQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGFYSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNRKSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTENLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL
Homology
BLAST of MELO3C031619.jh1 vs. NCBI nr
Match: XP_008438961.1 (PREDICTED: probable pectinesterase 67 [Cucumis melo] >KAA0049551.1 putative pectinesterase 67 [Cucumis melo var. makuwa] >TYK16228.1 putative pectinesterase 67 [Cucumis melo var. makuwa])

HSP 1 Score: 706 bits (1821), Expect = 1.81e-256
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI
Sbjct: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW
Sbjct: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180
           SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF
Sbjct: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300
           KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE
Sbjct: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300

Query: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350
           NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL
Sbjct: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350

BLAST of MELO3C031619.jh1 vs. NCBI nr
Match: XP_011651086.1 (probable pectinesterase 67 [Cucumis sativus] >KGN57171.1 hypothetical protein Csa_009846 [Cucumis sativus])

HSP 1 Score: 668 bits (1724), Expect = 1.10e-241
Identity = 328/350 (93.71%), Postives = 336/350 (96.00%), Query Frame = 0

Query: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSLFIKPIYIAF IAF FLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI
Sbjct: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW
Sbjct: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180
           SQSSSDNVESATFKVEA +FIAFG+SFKNEAPTGVAYTSQNQSVAAFVAADK AFYHCGF
Sbjct: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300
           KSANENSGFVFIKGKVYGVG TYLGRAKGA SRV+FAKTY SI+VVPAGWTNWS+VGSTE
Sbjct: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300

Query: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350
           NLYH EYDC GPGSES +RAPWAKQLTKEE  P M+V+FIDGTDWLPAWL
Sbjct: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350

BLAST of MELO3C031619.jh1 vs. NCBI nr
Match: XP_038888955.1 (probable pectinesterase 67 [Benincasa hispida])

HSP 1 Score: 631 bits (1627), Expect = 6.45e-227
Identity = 311/350 (88.86%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSS SLF  PI+IAFVIAFA LI SPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI
Sbjct: 1   MADSSSKSLF-NPIHIAFVIAFALLILSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEF SIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW
Sbjct: 61  NGNGEFTSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180
           SQSSSDN+ESATFKVEAQ+FIAFGISFKNEAPTGVAYTSQNQSVAAFVAADK +FYHCGF
Sbjct: 121 SQSSSDNIESATFKVEAQNFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKISFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYY +CYIQGSIDFIFGR +SVFHNCEMFV+DDKR TIRGSITAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYHNCYIQGSIDFIFGRSRSVFHNCEMFVVDDKRSTIRGSITAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300
           KS+NENSGFVFI+GKVYG+G  YLGRAKGA SRV+FAKTY S TV+PAGWTNWSY GSTE
Sbjct: 241 KSSNENSGFVFIRGKVYGIGGVYLGRAKGAFSRVIFAKTYFSKTVLPAGWTNWSYDGSTE 300

Query: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350
           NL+HAEY C GPGS S  RAPWAKQLTKEE  P M+V FIDGTDWLPAWL
Sbjct: 301 NLFHAEYACHGPGSASGHRAPWAKQLTKEEAQPFMEVGFIDGTDWLPAWL 349

BLAST of MELO3C031619.jh1 vs. NCBI nr
Match: XP_022979848.1 (probable pectinesterase 67 [Cucurbita maxima])

HSP 1 Score: 629 bits (1622), Expect = 3.73e-226
Identity = 303/350 (86.57%), Postives = 326/350 (93.14%), Query Frame = 0

Query: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSL  KPI+IAF IAFA LISSPIGSY FTAQNVI+SPLLTKKI TNRTIKVDI
Sbjct: 1   MADSSSNSL-CKPIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEF SIQAAVD+VPEGNSQWMIIHVRKGIYREKVH+PSSKPY+FLRGNGKGRT IVW
Sbjct: 61  NGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYVFLRGNGKGRTHIVW 120

Query: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180
           SQSS+DN+ESATFKVEA+HFIAFGISFKNEAPTGVAYTSQNQSVAAFVAAD  AFYHCGF
Sbjct: 121 SQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADNVAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCE+FVIDDKRLT+RGS+TAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEIFVIDDKRLTVRGSVTAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300
           KSA+ENSGFVFI+GKVYG+G  YLGRAKGA SRVVFAKTY S+TV+PAGWTNWSY G TE
Sbjct: 241 KSADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYAGGTE 300

Query: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350
           NLYH+EYDC GPG+E  +RAPWAKQLT++E A  MD+SFI+G DWLPAWL
Sbjct: 301 NLYHSEYDCHGPGAEKGNRAPWAKQLTEKEAAQFMDISFIEGADWLPAWL 349

BLAST of MELO3C031619.jh1 vs. NCBI nr
Match: KAG6582165.1 (putative pectinesterase 67, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 627 bits (1616), Expect = 3.06e-225
Identity = 303/350 (86.57%), Postives = 324/350 (92.57%), Query Frame = 0

Query: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSL   PI+IAF IAFA LISSPIGSY FTAQNVI+SPLLTKKI TNRTIKVDI
Sbjct: 1   MADSSSNSL-CNPIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEF SIQAAVD+VPEGNSQWMIIHVRKGIYREKVH+PSSKPYIFLRGNGKGRT +VW
Sbjct: 61  NGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYIFLRGNGKGRTHMVW 120

Query: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180
           SQSS+DN+ESATFKVEA+HFIAFGISFKNEAPTGVAYTSQNQSVAAF+AADK AFYHCGF
Sbjct: 121 SQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLT+RGS+TAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300
           KSA+ENSGFVFI+GKVYG+G  YLGRAKGA SRVVFAKTY S+TV+PAGWTNWSY G TE
Sbjct: 241 KSADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYSGGTE 300

Query: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350
           NLYH+EYDC GPGSE  +RAPWAKQLT +E    MD+SFI+G DWLPAWL
Sbjct: 301 NLYHSEYDCHGPGSEKGNRAPWAKQLTDKEADQFMDISFIEGADWLPAWL 349

BLAST of MELO3C031619.jh1 vs. ExPASy Swiss-Prot
Match: Q9LSP1 (Probable pectinesterase 67 OS=Arabidopsis thaliana OX=3702 GN=PME67 PE=2 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 2.5e-131
Identity = 220/333 (66.07%), Postives = 265/333 (79.58%), Query Frame = 0

Query: 19  VIAFAFLISSPIGSYAFTAQNV-IDSPLLTKKIGTNRTIKVDINGNGEFKSIQAAVDSVP 78
           ++    ++ S  GS A   Q    D+PLLT+KI TNR+I VDI G G++ S+Q A+D+VP
Sbjct: 8   ILVLTLVVMSIWGSDASAMQKTKFDAPLLTEKIATNRSIIVDIEGKGDYTSVQKAIDAVP 67

Query: 79  EGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVWSQSSSDNVESATFKVEA 138
            GNS W+I+HVRKGIY+E+VHIP +KP+IF+RGNGKG+T I  SQSS DNV SATFKVEA
Sbjct: 68  VGNSNWIIVHVRKGIYKERVHIPENKPFIFMRGNGKGKTVIESSQSSVDNVASATFKVEA 127

Query: 139 QHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGFYSTHNTLFDYKGRHYYD 198
            HF+AFGIS +N+AP G+A+TS+NQSVAAFVAADK AFYHC FYS HNTLFD KGRHYY 
Sbjct: 128 NHFVAFGISIRNDAPVGMAFTSENQSVAAFVAADKVAFYHCAFYSLHNTLFDNKGRHYYH 187

Query: 199 SCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNRKSANENSGFVFIKGKVY 258
            CYIQGSIDFIFGR  S+F+NCE+FVI DKR+   GSITA +R+SA E +G+VFI+GKVY
Sbjct: 188 ECYIQGSIDFIFGRATSIFNNCEIFVISDKRVKPYGSITAHHRESAEEKTGYVFIRGKVY 247

Query: 259 GVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTENLYHAEYDCRGPGSESK 318
           G+   YLGRAKG +SRV+FAKTYLS TVVP GWTNWSY GST+NLYH EY C GPG+E +
Sbjct: 248 GIDEVYLGRAKGPYSRVIFAKTYLSKTVVPDGWTNWSYHGSTQNLYHGEYKCHGPGAERQ 307

Query: 319 SRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 351
            R+ WAK LTK+EV   + + FIDGT WLP WL
Sbjct: 308 KRSDWAKDLTKQEVESFLSIDFIDGTSWLPVWL 340

BLAST of MELO3C031619.jh1 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.7e-66
Identity = 129/299 (43.14%), Postives = 186/299 (62.21%), Query Frame = 0

Query: 52  TNRTIKVDINGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGN 111
           T+  + VD++G G F ++Q+A+D VP+ +S   +I V  G YREKV +  +K  + ++G 
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 112 GKGRTSIVW---SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFV 171
           G   TSI W   ++S+ +  +S +F V A +F A+ ISFKN AP      +  Q+VA  +
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 172 AADKAAFYHCGFYSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKR 231
             D+AAFY CGFY   +TL D KGRH++  C+IQGSIDFIFG G+S++ +C +  I    
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 232 LT-IRGSITAQNRKSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVP 291
            + + GSITAQ R+S +E SGF F+  K+ G G   LGRA GA++ VVF+ TY+S  + P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 292 AGWTNWSYVGSTENLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWL 347
            GW NW      + +   E+ C GPG++ K R  + KQLT  E +  +DVSFIDG +WL
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWL 385

BLAST of MELO3C031619.jh1 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 7.1e-62
Identity = 122/308 (39.61%), Postives = 177/308 (57.47%), Query Frame = 0

Query: 49  KIGTNRTIKVDINGN-GEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIF 108
           K+  + T+ V    N G+F  IQ A+DS+P  N   ++I V  G+Y+EKV IP  K +I 
Sbjct: 78  KLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFIT 137

Query: 109 LRGNGKGRTSIVWSQSSS---------DNVESATFKVEAQHFIAFGISFKNEAPTGVAYT 168
           + G G  +T++ W  ++              SA+F V +  F+A  I+F+N  P  +   
Sbjct: 138 IEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGA 197

Query: 169 SQNQSVAAFVAADKAAFYHCGFYSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHN 228
              Q+VA  V+AD AAF+ C      +TL+D+ GRHYY  CYI+GS+DFIFG   S++  
Sbjct: 198 VGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEG 257

Query: 229 CEMFVIDDKRLTIRGSITAQNRKSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAK 288
           C +  I DK     G++TAQ R S  E++GF F+K KV G G  YLGRA G  SRVVFA 
Sbjct: 258 CHVHAIADK----LGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAY 317

Query: 289 TYLSITVVPAGWTNWSYVGSTENLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVS 347
           TY+   ++P GW NW        +++ +Y C G G+    R  WA++LT EE  P + ++
Sbjct: 318 TYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLT 377

BLAST of MELO3C031619.jh1 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 2.7e-61
Identity = 126/293 (43.00%), Postives = 170/293 (58.02%), Query Frame = 0

Query: 56  IKVDINGNGEFKSIQAAVDSVPE--GNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGK 115
           I+VD +G G+F  IQ A++S+P    NSQ   I V+ GIYREKV IP+ KPYI L G   
Sbjct: 51  IRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQA 110

Query: 116 GRTSIVWSQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKA 175
             T ++WS    D +ES T  + A  F+   ++ +N+      + +  ++VA  VAADKA
Sbjct: 111 SNTFLIWS-DGEDILESPTLTIFASDFVCRFLTIQNK------FGTAGRAVALRVAADKA 170

Query: 176 AFYHCGFYSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRG 235
           AFY C   S  +TL D  G HY+ +CYI+G+ DFI G   S++  C +  +        G
Sbjct: 171 AFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPN----NG 230

Query: 236 SITAQNRKSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNW 295
           SITAQ R SA E SGF F+  K+ G GST+LGR  GA+SRVVFA ++ S  V P GW  W
Sbjct: 231 SITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQW 290

Query: 296 SYVGSTENLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWL 347
                   +Y+ EY C GPG++ + R  W+KQL+ EE    +   FI G DWL
Sbjct: 291 GDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWL 332

BLAST of MELO3C031619.jh1 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 6.6e-60
Identity = 118/304 (38.82%), Postives = 176/304 (57.89%), Query Frame = 0

Query: 53  NRTIKVDINGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNG 112
           ++ I V +NG+ +F+S+Q AVDS+P+ N++ + I +  G YREKV +P++KPYI  +G G
Sbjct: 58  HKVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAG 117

Query: 113 KGRTSIVWSQSSSD---------NVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQS 172
           +  T+I W   +SD           ++A+  V A +F A  ISF N AP  +      Q+
Sbjct: 118 RDVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQA 177

Query: 173 VAAFVAADKAAFYHCGFYSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFV 232
           VA  ++ DKA F  CGFY   +TL D  GRHY+  CYI+GSIDFIFG G+S++ +CE+  
Sbjct: 178 VAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHS 237

Query: 233 IDDKRLTIRGSITAQNRKSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSI 292
           I  +     GSI A  R    E +GF F+  +V G G  Y+GRA G +SR+V+A TY   
Sbjct: 238 IASR----FGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDA 297

Query: 293 TVVPAGWTNWSYVGS-TENLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDG 347
            V   GW +W +  + ++  +   Y+C GPG+ +     WA+ L  E   P +  SF++G
Sbjct: 298 LVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNG 357

BLAST of MELO3C031619.jh1 vs. ExPASy TrEMBL
Match: A0A5A7U7L5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001570 PE=4 SV=1)

HSP 1 Score: 706 bits (1821), Expect = 8.75e-257
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI
Sbjct: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW
Sbjct: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180
           SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF
Sbjct: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300
           KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE
Sbjct: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300

Query: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350
           NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL
Sbjct: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350

BLAST of MELO3C031619.jh1 vs. ExPASy TrEMBL
Match: A0A1S3AY88 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483896 PE=4 SV=1)

HSP 1 Score: 706 bits (1821), Expect = 8.75e-257
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI
Sbjct: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW
Sbjct: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180
           SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF
Sbjct: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300
           KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE
Sbjct: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300

Query: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350
           NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL
Sbjct: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350

BLAST of MELO3C031619.jh1 vs. ExPASy TrEMBL
Match: A0A0A0LAR0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G166360 PE=4 SV=1)

HSP 1 Score: 668 bits (1724), Expect = 5.35e-242
Identity = 328/350 (93.71%), Postives = 336/350 (96.00%), Query Frame = 0

Query: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSLFIKPIYIAF IAF FLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI
Sbjct: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW
Sbjct: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180
           SQSSSDNVESATFKVEA +FIAFG+SFKNEAPTGVAYTSQNQSVAAFVAADK AFYHCGF
Sbjct: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300
           KSANENSGFVFIKGKVYGVG TYLGRAKGA SRV+FAKTY SI+VVPAGWTNWS+VGSTE
Sbjct: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300

Query: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350
           NLYH EYDC GPGSES +RAPWAKQLTKEE  P M+V+FIDGTDWLPAWL
Sbjct: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350

BLAST of MELO3C031619.jh1 vs. ExPASy TrEMBL
Match: A0A6J1IXH1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479420 PE=4 SV=1)

HSP 1 Score: 629 bits (1622), Expect = 1.80e-226
Identity = 303/350 (86.57%), Postives = 326/350 (93.14%), Query Frame = 0

Query: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSL  KPI+IAF IAFA LISSPIGSY FTAQNVI+SPLLTKKI TNRTIKVDI
Sbjct: 1   MADSSSNSL-CKPIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEF SIQAAVD+VPEGNSQWMIIHVRKGIYREKVH+PSSKPY+FLRGNGKGRT IVW
Sbjct: 61  NGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYVFLRGNGKGRTHIVW 120

Query: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180
           SQSS+DN+ESATFKVEA+HFIAFGISFKNEAPTGVAYTSQNQSVAAFVAAD  AFYHCGF
Sbjct: 121 SQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADNVAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCE+FVIDDKRLT+RGS+TAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEIFVIDDKRLTVRGSVTAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300
           KSA+ENSGFVFI+GKVYG+G  YLGRAKGA SRVVFAKTY S+TV+PAGWTNWSY G TE
Sbjct: 241 KSADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYAGGTE 300

Query: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350
           NLYH+EYDC GPG+E  +RAPWAKQLT++E A  MD+SFI+G DWLPAWL
Sbjct: 301 NLYHSEYDCHGPGAEKGNRAPWAKQLTEKEAAQFMDISFIEGADWLPAWL 349

BLAST of MELO3C031619.jh1 vs. ExPASy TrEMBL
Match: A0A6J1GU67 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457552 PE=4 SV=1)

HSP 1 Score: 624 bits (1609), Expect = 1.73e-224
Identity = 301/350 (86.00%), Postives = 324/350 (92.57%), Query Frame = 0

Query: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSL   PI+IAF IAFA LISSPIGSY FTAQNVI+SPLLTKKI TNRTIKVDI
Sbjct: 1   MADSSSNSL-CNPIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEF SIQAAVD+VPEGNSQWMIIHVRKGIYREKVH+PSSKPYIFLRGNGKGRT +VW
Sbjct: 61  NGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYIFLRGNGKGRTHMVW 120

Query: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180
           SQSS+DN+ESATFKVEA+HFIAFGISFKNEAPTGVAYTSQNQSVAAF+AADK AFYHCGF
Sbjct: 121 SQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRL++RGS+TAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLSVRGSVTAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300
           KSA+ENSGFVFI+GKVYG+G  YLGRAKGA SRVVFAKTY S+TV+PAGWTNWSY G TE
Sbjct: 241 KSADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYSGGTE 300

Query: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350
           NLYH+EYDC GPG+E  +RAPWAKQLT +E    MD+SFI+G DWLPAWL
Sbjct: 301 NLYHSEYDCHGPGAEKGNRAPWAKQLTDKEADQFMDISFIEGADWLPAWL 349

BLAST of MELO3C031619.jh1 vs. TAIR 10
Match: AT3G17060.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 469.9 bits (1208), Expect = 1.7e-132
Identity = 220/333 (66.07%), Postives = 265/333 (79.58%), Query Frame = 0

Query: 19  VIAFAFLISSPIGSYAFTAQNV-IDSPLLTKKIGTNRTIKVDINGNGEFKSIQAAVDSVP 78
           ++    ++ S  GS A   Q    D+PLLT+KI TNR+I VDI G G++ S+Q A+D+VP
Sbjct: 8   ILVLTLVVMSIWGSDASAMQKTKFDAPLLTEKIATNRSIIVDIEGKGDYTSVQKAIDAVP 67

Query: 79  EGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVWSQSSSDNVESATFKVEA 138
            GNS W+I+HVRKGIY+E+VHIP +KP+IF+RGNGKG+T I  SQSS DNV SATFKVEA
Sbjct: 68  VGNSNWIIVHVRKGIYKERVHIPENKPFIFMRGNGKGKTVIESSQSSVDNVASATFKVEA 127

Query: 139 QHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGFYSTHNTLFDYKGRHYYD 198
            HF+AFGIS +N+AP G+A+TS+NQSVAAFVAADK AFYHC FYS HNTLFD KGRHYY 
Sbjct: 128 NHFVAFGISIRNDAPVGMAFTSENQSVAAFVAADKVAFYHCAFYSLHNTLFDNKGRHYYH 187

Query: 199 SCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNRKSANENSGFVFIKGKVY 258
            CYIQGSIDFIFGR  S+F+NCE+FVI DKR+   GSITA +R+SA E +G+VFI+GKVY
Sbjct: 188 ECYIQGSIDFIFGRATSIFNNCEIFVISDKRVKPYGSITAHHRESAEEKTGYVFIRGKVY 247

Query: 259 GVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTENLYHAEYDCRGPGSESK 318
           G+   YLGRAKG +SRV+FAKTYLS TVVP GWTNWSY GST+NLYH EY C GPG+E +
Sbjct: 248 GIDEVYLGRAKGPYSRVIFAKTYLSKTVVPDGWTNWSYHGSTQNLYHGEYKCHGPGAERQ 307

Query: 319 SRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 351
            R+ WAK LTK+EV   + + FIDGT WLP WL
Sbjct: 308 KRSDWAKDLTKQEVESFLSIDFIDGTSWLPVWL 340

BLAST of MELO3C031619.jh1 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 253.1 bits (645), Expect = 3.4e-67
Identity = 129/299 (43.14%), Postives = 186/299 (62.21%), Query Frame = 0

Query: 52  TNRTIKVDINGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGN 111
           T+  + VD++G G F ++Q+A+D VP+ +S   +I V  G YREKV +  +K  + ++G 
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 112 GKGRTSIVW---SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFV 171
           G   TSI W   ++S+ +  +S +F V A +F A+ ISFKN AP      +  Q+VA  +
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 172 AADKAAFYHCGFYSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKR 231
             D+AAFY CGFY   +TL D KGRH++  C+IQGSIDFIFG G+S++ +C +  I    
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 232 LT-IRGSITAQNRKSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVP 291
            + + GSITAQ R+S +E SGF F+  K+ G G   LGRA GA++ VVF+ TY+S  + P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 292 AGWTNWSYVGSTENLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWL 347
            GW NW      + +   E+ C GPG++ K R  + KQLT  E +  +DVSFIDG +WL
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWL 385

BLAST of MELO3C031619.jh1 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 239.2 bits (609), Expect = 5.0e-63
Identity = 122/308 (39.61%), Postives = 177/308 (57.47%), Query Frame = 0

Query: 49  KIGTNRTIKVDINGN-GEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIF 108
           K+  + T+ V    N G+F  IQ A+DS+P  N   ++I V  G+Y+EKV IP  K +I 
Sbjct: 78  KLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFIT 137

Query: 109 LRGNGKGRTSIVWSQSSS---------DNVESATFKVEAQHFIAFGISFKNEAPTGVAYT 168
           + G G  +T++ W  ++              SA+F V +  F+A  I+F+N  P  +   
Sbjct: 138 IEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGA 197

Query: 169 SQNQSVAAFVAADKAAFYHCGFYSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHN 228
              Q+VA  V+AD AAF+ C      +TL+D+ GRHYY  CYI+GS+DFIFG   S++  
Sbjct: 198 VGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEG 257

Query: 229 CEMFVIDDKRLTIRGSITAQNRKSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAK 288
           C +  I DK     G++TAQ R S  E++GF F+K KV G G  YLGRA G  SRVVFA 
Sbjct: 258 CHVHAIADK----LGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAY 317

Query: 289 TYLSITVVPAGWTNWSYVGSTENLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVS 347
           TY+   ++P GW NW        +++ +Y C G G+    R  WA++LT EE  P + ++
Sbjct: 318 TYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLT 377

BLAST of MELO3C031619.jh1 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 237.3 bits (604), Expect = 1.9e-62
Identity = 126/293 (43.00%), Postives = 170/293 (58.02%), Query Frame = 0

Query: 56  IKVDINGNGEFKSIQAAVDSVPE--GNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGK 115
           I+VD +G G+F  IQ A++S+P    NSQ   I V+ GIYREKV IP+ KPYI L G   
Sbjct: 51  IRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQA 110

Query: 116 GRTSIVWSQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKA 175
             T ++WS    D +ES T  + A  F+   ++ +N+      + +  ++VA  VAADKA
Sbjct: 111 SNTFLIWS-DGEDILESPTLTIFASDFVCRFLTIQNK------FGTAGRAVALRVAADKA 170

Query: 176 AFYHCGFYSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRG 235
           AFY C   S  +TL D  G HY+ +CYI+G+ DFI G   S++  C +  +        G
Sbjct: 171 AFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPN----NG 230

Query: 236 SITAQNRKSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNW 295
           SITAQ R SA E SGF F+  K+ G GST+LGR  GA+SRVVFA ++ S  V P GW  W
Sbjct: 231 SITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQW 290

Query: 296 SYVGSTENLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWL 347
                   +Y+ EY C GPG++ + R  W+KQL+ EE    +   FI G DWL
Sbjct: 291 GDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWL 332

BLAST of MELO3C031619.jh1 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 232.6 bits (592), Expect = 4.7e-61
Identity = 118/304 (38.82%), Postives = 176/304 (57.89%), Query Frame = 0

Query: 53  NRTIKVDINGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNG 112
           ++ I V +NG+ +F+S+Q AVDS+P+ N++ + I +  G YREKV +P++KPYI  +G G
Sbjct: 58  HKVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAG 117

Query: 113 KGRTSIVWSQSSSD---------NVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQS 172
           +  T+I W   +SD           ++A+  V A +F A  ISF N AP  +      Q+
Sbjct: 118 RDVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQA 177

Query: 173 VAAFVAADKAAFYHCGFYSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFV 232
           VA  ++ DKA F  CGFY   +TL D  GRHY+  CYI+GSIDFIFG G+S++ +CE+  
Sbjct: 178 VAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHS 237

Query: 233 IDDKRLTIRGSITAQNRKSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSI 292
           I  +     GSI A  R    E +GF F+  +V G G  Y+GRA G +SR+V+A TY   
Sbjct: 238 IASR----FGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDA 297

Query: 293 TVVPAGWTNWSYVGS-TENLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDG 347
            V   GW +W +  + ++  +   Y+C GPG+ +     WA+ L  E   P +  SF++G
Sbjct: 298 LVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNG 357

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008438961.11.81e-256100.00PREDICTED: probable pectinesterase 67 [Cucumis melo] >KAA0049551.1 putative pect... [more]
XP_011651086.11.10e-24193.71probable pectinesterase 67 [Cucumis sativus] >KGN57171.1 hypothetical protein Cs... [more]
XP_038888955.16.45e-22788.86probable pectinesterase 67 [Benincasa hispida][more]
XP_022979848.13.73e-22686.57probable pectinesterase 67 [Cucurbita maxima][more]
KAG6582165.13.06e-22586.57putative pectinesterase 67, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9LSP12.5e-13166.07Probable pectinesterase 67 OS=Arabidopsis thaliana OX=3702 GN=PME67 PE=2 SV=1[more]
Q9ZQA34.7e-6643.14Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
Q8VYZ37.1e-6239.61Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
Q9SIJ92.7e-6143.00Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1[more]
Q8LPF36.6e-6038.82Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7U7L58.75e-257100.00Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00157... [more]
A0A1S3AY888.75e-257100.00Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483896 PE=4 SV=1[more]
A0A0A0LAR05.35e-24293.71Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G166360 PE=4 SV=1[more]
A0A6J1IXH11.80e-22686.57Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479420 PE=4 SV=1[more]
A0A6J1GU671.73e-22486.00Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457552 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G17060.11.7e-13266.07Pectin lyase-like superfamily protein [more]
AT2G36710.13.4e-6743.14Pectin lyase-like superfamily protein [more]
AT5G19730.15.0e-6339.61Pectin lyase-like superfamily protein [more]
AT2G21610.11.9e-6243.00pectinesterase 11 [more]
AT5G47500.14.7e-6138.82Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 50..349
e-value: 2.7E-95
score: 321.4
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 57..342
e-value: 1.3E-62
score: 211.2
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 14..349
NoneNo IPR availablePANTHERPTHR31321:SF98PECTINESTERASE 67-RELATEDcoord: 14..349
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 201..210
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 52..348

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C031619.jh1.t1MELO3C031619.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
molecular_function GO:0030599 pectinesterase activity