MELO3C030359 (gene) Melon (DHL92) v4

Overview


Name	MELO3C030359
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	Beta-amyrin 11-oxidase-like
Location	chr04: 571356 .. 571657 (+)
RNA-Seq Expression	MELO3C030359
Synteny	MELO3C030359

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCACAAAACGGCCTATAGAATAATGGCGTCTTCCTTGAATGGGTATGAGATATTATCTAAACATGTGAGTTTCATTGATGAAATAATGAGTAAAGGGTTAGAGGAATGGAGCACAATGAAAGAACCCATTGAATTGTTGAGTGAAATTGGAAGTTTACTCTTTAAAGCCATTGTTCGTATCTTTTTAGGGAATGAAATTCCTATCCCTACATTGAATAAGCTTGAAGCGATGTACAAACACTTGGGTCCAGCAATTTTGTCCATTCTTCCTTACGATTTGCCTGTCACTCAAGGTAT

mRNA sequence

ATGGATCACAAAACGGCCTATAGAATAATGGCGTCTTCCTTGAATGGGTATGAGATATTATCTAAACATGTGAGTTTCATTGATGAAATAATGAGTAAAGGGTTAGAGGAATGGAGCACAATGAAAGAACCCATTGAATTGTTGAGTGAAATTGGAAGTTTACTCTTTAAAGCCATTGTTCGTATCTTTTTAGGGAATGAAATTCCTATCCCTACATTGAATAAGCTTGAAGCGATGTACAAACACTTGGGTCCAGCAATTTTGTCCATTCTTCCTTACGATTTGCCTGTCACTCAAGGTAT

Coding sequence (CDS)

ATGGATCACAAAACGGCCTATAGAATAATGGCGTCTTCCTTGAATGGGTATGAGATATTATCTAAACATGTGAGTTTCATTGATGAAATAATGAGTAAAGGGTTAGAGGAATGGAGCACAATGAAAGAACCCATTGAATTGTTGAGTGAAATTGGAAGTTTACTCTTTAAAGCCATTGTTCGTATCTTTTTAGGGAATGAAATTCCTATCCCTACATTGAATAAGCTTGAAGCGATGTACAAACACTTGGGTCCAGCAATTTTGTCCATTCTTCCTTACGATTTGCCTGTCACTCAAGGT

Protein sequence

MDHKTAYRIMASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIVRIFLGNEIPIPTLNKLEAMYKHLGPAILSILPYDLPVTQG

Homology

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BLAST of MELO3C030359 vs. NCBI nr
Match: KAA0038542.1 (beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa] >TYK31139.1 beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa])

HSP 1 Score: 181.4 bits (459), Expect = 3.8e-42
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0


Query: 10  MASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIVRIFLGNEIP 69
           MASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIVRIFLGNEIP
Sbjct: 1   MASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIVRIFLGNEIP 60

Query: 70  IPTLNKLEAMYKHLGPAILSILPYDLPVTQG 101
           IPTLNKLEAMYKHLGPAILSILPYDLPVTQG
Sbjct: 61  IPTLNKLEAMYKHLGPAILSILPYDLPVTQG 91

[Close]

BLAST of MELO3C030359 vs. NCBI nr
Match: KAE8651451.1 (hypothetical protein Csa_000969 [Cucumis sativus])

HSP 1 Score: 174.9 bits (442), Expect = 3.6e-40
Identity = 85/96 (88.54%), Postives = 91/96 (94.79%), Query Frame = 0


Query: 1   MDHKTAYRIMASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIV 60
           +DHKT YRIMAS LNGYEILSK+VSFI+EIMSKGLEEWSTMKEPIELLSEIGSLLFK I+
Sbjct: 132 IDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLSEIGSLLFKVII 191

Query: 61  RIFLGNEIPIPTLNKLEAMYKHLGPAILSILPYDLP 97
           RIFLGNEIP PTLNKLEA+YKHLGP ILSILPYD+P
Sbjct: 192 RIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVP 227

[Close]

BLAST of MELO3C030359 vs. NCBI nr
Match: XP_011652661.1 (beta-amyrin 11-oxidase [Cucumis sativus])

HSP 1 Score: 174.9 bits (442), Expect = 3.6e-40
Identity = 85/96 (88.54%), Postives = 91/96 (94.79%), Query Frame = 0


Query: 1   MDHKTAYRIMASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIV 60
           +DHKT YRIMAS LNGYEILSK+VSFI+EIMSKGLEEWSTMKEPIELLSEIGSLLFK I+
Sbjct: 132 IDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLSEIGSLLFKVII 191

Query: 61  RIFLGNEIPIPTLNKLEAMYKHLGPAILSILPYDLP 97
           RIFLGNEIP PTLNKLEA+YKHLGP ILSILPYD+P
Sbjct: 192 RIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVP 227

[Close]

BLAST of MELO3C030359 vs. NCBI nr
Match: XP_016903531.1 (PREDICTED: beta-amyrin 11-oxidase-like [Cucumis melo])

HSP 1 Score: 172.2 bits (435), Expect = 2.3e-39
Identity = 83/96 (86.46%), Postives = 91/96 (94.79%), Query Frame = 0


Query: 1   MDHKTAYRIMASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIV 60
           MDHKTAY+IMAS LNGYEILSK+V+FID++MSKGLEEWSTMKEPIELLSEIGS+LFK I+
Sbjct: 109 MDHKTAYKIMASPLNGYEILSKYVTFIDKLMSKGLEEWSTMKEPIELLSEIGSILFKVII 168

Query: 61  RIFLGNEIPIPTLNKLEAMYKHLGPAILSILPYDLP 97
            IFLGNEIP  TLNKLEA+YKHLGPAILSILPYDLP
Sbjct: 169 SIFLGNEIPTTTLNKLEALYKHLGPAILSILPYDLP 204

[Close]

BLAST of MELO3C030359 vs. NCBI nr
Match: XP_038877829.1 (beta-amyrin 11-oxidase-like [Benincasa hispida])

HSP 1 Score: 160.2 bits (404), Expect = 9.1e-36
Identity = 77/96 (80.21%), Postives = 86/96 (89.58%), Query Frame = 0


Query: 1   MDHKTAYRIMASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIV 60
           +DHKTAY+IMAS +NGYEILS +VSFID+ M+KGLEEWST KEPIELLSEIGSLLFK I+
Sbjct: 131 IDHKTAYKIMASPINGYEILSNYVSFIDQTMAKGLEEWSTAKEPIELLSEIGSLLFKVII 190

Query: 61  RIFLGNEIPIPTLNKLEAMYKHLGPAILSILPYDLP 97
            IFLGNEI  PT+ KLE +YKHLGPAILSILPYDLP
Sbjct: 191 HIFLGNEITAPTMAKLEELYKHLGPAILSILPYDLP 226

[Close]

BLAST of MELO3C030359 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.1e-07
Identity = 31/95 (32.63%), Postives = 58/95 (61.05%), Query Frame = 0


Query: 2   DHKTAYRIMASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIVR 61
           +H+   R++ S + G++ L+ ++  ++EI+   LEE S+MK P+ELL E+  + FKAIV 
Sbjct: 137 EHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFKAIVH 196

Query: 62  IFLGNEIPIPTLNKLEAMYKHLGPAILSILPYDLP 97
           +F+G+      + K+ + +  L   + SI P ++P
Sbjct: 197 VFMGSSNQ-DIIKKIGSSFTDLYNGMFSI-PINVP 229

[Close]

BLAST of MELO3C030359 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 6.0e-06
Identity = 27/83 (32.53%), Postives = 48/83 (57.83%), Query Frame = 0


Query: 2   DHKTAYRIMASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKE-PIELLSEIGSLLFKAIV 61
           DH+   ++ A+ +NG++ L+ ++SFID+ +   L  WS+ +   +E L+E+  + FK IV
Sbjct: 149 DHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMTFKIIV 208

Query: 62  RIFLGNEIPIPTLNKLEAMYKHL 84
           +IF+       T+  LE  Y  L
Sbjct: 209 QIFMSG-ADDATMEALERSYTDL 230

[Close]

BLAST of MELO3C030359 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 7.8e-06
Identity = 26/82 (31.71%), Postives = 45/82 (54.88%), Query Frame = 0


Query: 2   DHKTAYRIMASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIVR 61
           +H+   ++ A+ +NG++ L+ ++ FID  ++  L  W+     +E L+E+  + FK IV+
Sbjct: 166 EHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKIIVQ 225

Query: 62  IFLGNEIPIPTLNKLEAMYKHL 84
           IFLG      T   LE  Y  L
Sbjct: 226 IFLGG-ADQATTRALERSYTEL 246

[Close]

BLAST of MELO3C030359 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 44.3 bits (103), Expect = 9.5e-04
Identity = 28/97 (28.87%), Postives = 50/97 (51.55%), Query Frame = 0


Query: 2   DHKTAYRIMASSLNGYEILSKHVSFIDEIMSKGLEEWSTM--KEPIELLSEIGSLLFKAI 61
           +H+   ++ A+ +NG++ L+ ++ FID+ +   L  WS        E L+E+  + F+ I
Sbjct: 139 EHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRII 198

Query: 62  VRIFLGNEIPIPTLNKLEAMYKHLGPAILSILPYDLP 97
           V+IF+G      T  +LE  Y  L    +  +  DLP
Sbjct: 199 VQIFMGG-ADERTAAELERTYTELNYG-MRAMAIDLP 233

[Close]

BLAST of MELO3C030359 vs. ExPASy TrEMBL
Match: A0A5D3E5N1 (Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004190 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.8e-42
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0


Query: 10  MASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIVRIFLGNEIP 69
           MASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIVRIFLGNEIP
Sbjct: 1   MASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIVRIFLGNEIP 60

Query: 70  IPTLNKLEAMYKHLGPAILSILPYDLPVTQG 101
           IPTLNKLEAMYKHLGPAILSILPYDLPVTQG
Sbjct: 61  IPTLNKLEAMYKHLGPAILSILPYDLPVTQG 91

[Close]

BLAST of MELO3C030359 vs. ExPASy TrEMBL
Match: A0A0A0LI74 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G904060 PE=4 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.7e-40
Identity = 85/96 (88.54%), Postives = 91/96 (94.79%), Query Frame = 0


Query: 1   MDHKTAYRIMASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIV 60
           +DHKT YRIMAS LNGYEILSK+VSFI+EIMSKGLEEWSTMKEPIELLSEIGSLLFK I+
Sbjct: 129 IDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLSEIGSLLFKVII 188

Query: 61  RIFLGNEIPIPTLNKLEAMYKHLGPAILSILPYDLP 97
           RIFLGNEIP PTLNKLEA+YKHLGP ILSILPYD+P
Sbjct: 189 RIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVP 224

[Close]

BLAST of MELO3C030359 vs. ExPASy TrEMBL
Match: A0A1S4E5L3 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103503515 PE=3 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.1e-39
Identity = 83/96 (86.46%), Postives = 91/96 (94.79%), Query Frame = 0


Query: 1   MDHKTAYRIMASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIV 60
           MDHKTAY+IMAS LNGYEILSK+V+FID++MSKGLEEWSTMKEPIELLSEIGS+LFK I+
Sbjct: 109 MDHKTAYKIMASPLNGYEILSKYVTFIDKLMSKGLEEWSTMKEPIELLSEIGSILFKVII 168

Query: 61  RIFLGNEIPIPTLNKLEAMYKHLGPAILSILPYDLP 97
            IFLGNEIP  TLNKLEA+YKHLGPAILSILPYDLP
Sbjct: 169 SIFLGNEIPTTTLNKLEALYKHLGPAILSILPYDLP 204

[Close]

BLAST of MELO3C030359 vs. ExPASy TrEMBL
Match: A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.1e-30
Identity = 67/95 (70.53%), Postives = 80/95 (84.21%), Query Frame = 0


Query: 2   DHKTAYRIMASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIVR 61
           DHK+AYRIMA+ +NGYE+LS +V FID++M+KGLEEWS+MKEPIELL EIGSL FK I R
Sbjct: 132 DHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKVISR 191

Query: 62  IFLGNEIPIPTLNKLEAMYKHLGPAILSILPYDLP 97
           IFLG +I    + +LE +YK LGPAILSILPYDLP
Sbjct: 192 IFLGTDIGASRMAELETLYKDLGPAILSILPYDLP 226

[Close]

BLAST of MELO3C030359 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 4.7e-30
Identity = 66/95 (69.47%), Postives = 80/95 (84.21%), Query Frame = 0


Query: 2   DHKTAYRIMASSLNGYEILSKHVSFIDEIMSKGLEEWSTMKEPIELLSEIGSLLFKAIVR 61
           DHK+AYRIMA+ +NGYE+LS +V FID++M+KGLEEWS+MKEPIELL EIGSL FK I R
Sbjct: 132 DHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGEIGSLFFKVISR 191

Query: 62  IFLGNEIPIPTLNKLEAMYKHLGPAILSILPYDLP 97
           IFLG +I    + +LE +YK LGPAILSILPYD+P
Sbjct: 192 IFLGTDIGASRMAELETLYKDLGPAILSILPYDVP 226

The following BLAST results are available for this feature:

BLAST of MELO3C030359 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0038542.1	3.8e-42	100.00	beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa] >TYK31139.1 beta-amyrin 1...	[more]
KAE8651451.1	3.6e-40	88.54	hypothetical protein Csa_000969 [Cucumis sativus]	[more]
XP_011652661.1	3.6e-40	88.54	beta-amyrin 11-oxidase [Cucumis sativus]	[more]
XP_016903531.1	2.3e-39	86.46	PREDICTED: beta-amyrin 11-oxidase-like [Cucumis melo]	[more]
XP_038877829.1	9.1e-36	80.21	beta-amyrin 11-oxidase-like [Benincasa hispida]	[more]

BLAST of MELO3C030359 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	1.1e-07	32.63	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q5VRM7	6.0e-06	32.53	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q43246	7.8e-06	31.71	Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1	[more]
Q9AXH9	9.5e-04	28.87	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]

BLAST of MELO3C030359 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E5N1	1.8e-42	100.00	Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A0A0LI74	1.7e-40	88.54	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G904060 PE=4 SV=1	[more]
A0A1S4E5L3	1.1e-39	86.46	beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103503515 PE=3 SV=1	[more]
A0A6J1GLR1	2.1e-30	70.53	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 S...	[more]
A0A6J1GJM1	4.7e-30	69.47	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 S...	[more]

BLAST of MELO3C030359 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR24286:SF289	ENT-KAURENOIC ACID OXIDASE 2	coord: 2..96
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 2..96

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C030359.1	MELO3C030359.1	mRNA