MELO3C030350 (gene) Melon (DHL92) v4

BLAST of MELO3C030350 vs. NCBI nr
Match: XP_011652661.1 (beta-amyrin 11-oxidase [Cucumis sativus])

HSP 1 Score: 450.7 bits (1158), Expect = 9.3e-123
Identity = 223/266 (83.83%), Postives = 237/266 (89.10%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           ARKEI NIL+ VI EKRRVLER KET D+LV+CQMDKLIVA DENGK+LYDN AIIDL L
Sbjct: 237 ARKEIENILEGVIMEKRRVLER-KETNDELVKCQMDKLIVATDENGKKLYDNKAIIDLLL 296

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
           GLLHAGHHTPAY AMW +VQISQNSH+FQKAKEEQE IMKERPS+QKGLSF EIKQMKYL
Sbjct: 297 GLLHAGHHTPAYTAMWAIVQISQNSHIFQKAKEEQELIMKERPSTQKGLSFTEIKQMKYL 356

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
           MKF NELLRR+T+AVASFRE T NV+INDYTIPKGWT+ IWTRA+HMDPQIYSNPQEFDP
Sbjct: 357 MKFTNELLRRHTVAVASFREATANVHINDYTIPKGWTVTIWTRAIHMDPQIYSNPQEFDP 416

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERVN 252
           SRW+         NYTPKPGE+LPFGIGSRFCPGSELTKLYITILLH FIL NYKMERVN
Sbjct: 417 SRWD---------NYTPKPGEFLPFGIGSRFCPGSELTKLYITILLHQFIL-NYKMERVN 476

Query: 253 SRCDTTHLPAPKLVDNCLCRIIKLPY 279
            RCDTTHLPAPKLVDNCLCRIIKL Y
Sbjct: 477 QRCDTTHLPAPKLVDNCLCRIIKLSY 491

BLAST of MELO3C030350 vs. NCBI nr
Match: XP_038877829.1 (beta-amyrin 11-oxidase-like [Benincasa hispida])

HSP 1 Score: 440.3 bits (1131), Expect = 1.3e-119
Identity = 221/265 (83.40%), Postives = 237/265 (89.43%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           ARKEIGNILDCVIEEKRRVLE  KET++  V+ QMDKLIVA DENGK+LYDNN IIDL L
Sbjct: 236 ARKEIGNILDCVIEEKRRVLE-NKETRE--VKSQMDKLIVATDENGKKLYDNNDIIDLLL 295

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
           GLLHAGHHTPAYAAMW +VQIS+N+HVFQ  KEEQEFIMKERPS+QKGLSFKEIKQMKYL
Sbjct: 296 GLLHAGHHTPAYAAMWALVQISENAHVFQ--KEEQEFIMKERPSTQKGLSFKEIKQMKYL 355

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
           MKF+NELLRR+TIAVASFRE  T+VYINDY IPKGWT+ IWTRA+HMDPQIYSNPQEFDP
Sbjct: 356 MKFINELLRRHTIAVASFREAKTDVYINDYIIPKGWTVPIWTRAIHMDPQIYSNPQEFDP 415

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERVN 252
           SRW+         NYTPKPG ++PFGIGSRFCPGSELTKLYITILLHHFIL NYKMERVN
Sbjct: 416 SRWD---------NYTPKPGAFIPFGIGSRFCPGSELTKLYITILLHHFIL-NYKMERVN 475

Query: 253 SRCDTTHLPAPKLVDNCLCRIIKLP 278
            RCD THLPAPKLVDNCLCRIIKLP
Sbjct: 476 PRCDITHLPAPKLVDNCLCRIIKLP 485

BLAST of MELO3C030350 vs. NCBI nr
Match: XP_016903531.1 (PREDICTED: beta-amyrin 11-oxidase-like [Cucumis melo])

HSP 1 Score: 422.9 bits (1086), Expect = 2.1e-114
Identity = 210/237 (88.61%), Postives = 218/237 (91.98%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           ARKEIGNIL+CVIEEKRRVLER KETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDL L
Sbjct: 214 ARKEIGNILECVIEEKRRVLERNKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLLL 273

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
           GLLHAGHHTPAYAA W VVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL
Sbjct: 274 GLLHAGHHTPAYAATWAVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 333

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
           MKFVNELLRRY IAVASFRE TTNVYINDYTIPKGW + IWTRA+HMDPQ+YSNPQEFDP
Sbjct: 334 MKFVNELLRRYAIAVASFREATTNVYINDYTIPKGWIVTIWTRAIHMDPQMYSNPQEFDP 393

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKME 250
           SRW+         NYTPKPGE++PFG+GSRFCPGSELTKL ITILLHHFIL NYKME
Sbjct: 394 SRWD---------NYTPKPGEFIPFGLGSRFCPGSELTKLEITILLHHFIL-NYKME 440

BLAST of MELO3C030350 vs. NCBI nr
Match: XP_023511598.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 397.5 bits (1020), Expect = 9.4e-107
Identity = 194/265 (73.21%), Postives = 226/265 (85.28%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           ARK+I N+L+CVIEEKRR LE KKET++  V  QMDKLI+A DENG +LYDNNAIIDL L
Sbjct: 84  ARKKIENVLECVIEEKRRALE-KKETRE--VHSQMDKLIMARDENGMKLYDNNAIIDLLL 143

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
           GLLH GHHTPAYAAMW +V IS+N H+F +AKEEQE I+K+RPS+QKGL+F+EIKQMKYL
Sbjct: 144 GLLHGGHHTPAYAAMWALVHISENPHIFYEAKEEQESILKQRPSTQKGLTFQEIKQMKYL 203

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
           MKF+NELLRR TIAVASFR+  T++ INDYTIPKGW ++IWTRA+HMDP +YSNPQEFDP
Sbjct: 204 MKFINELLRRNTIAVASFRKAKTDININDYTIPKGWNVLIWTRAIHMDPHLYSNPQEFDP 263

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERVN 252
           SRW         +N++PKPG ++PFGIG+RFCPGSELTKL ITILLHHFIL NYKMERVN
Sbjct: 264 SRW---------ENHSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFIL-NYKMERVN 323

Query: 253 SRCDTTHLPAPKLVDNCLCRIIKLP 278
            +C  +HLPAPKLVDNCLC I KLP
Sbjct: 324 PKCYISHLPAPKLVDNCLCTITKLP 335

BLAST of MELO3C030350 vs. NCBI nr
Match: XP_023511599.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 397.5 bits (1020), Expect = 9.4e-107
Identity = 192/265 (72.45%), Postives = 226/265 (85.28%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           ARK+I NIL CVIEEKRR LE+    +++ V+ QMDKLI+A DENG +LYDNNAIIDL L
Sbjct: 236 ARKKIENILQCVIEEKRRALEK----QEREVESQMDKLIMARDENGMKLYDNNAIIDLLL 295

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
           GLLH GHHTPAYAAMW +V IS+N H+F +AKEEQE I+K+RPS+QKGL+F++IKQMKYL
Sbjct: 296 GLLHGGHHTPAYAAMWALVHISENPHIFYQAKEEQESILKQRPSTQKGLTFQKIKQMKYL 355

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
           MKF+NELLRR TIAVASFRE  T++ INDYTIPKGWT++IWTRA+HMDPQ+YSNP+EFDP
Sbjct: 356 MKFINELLRRNTIAVASFREAKTDININDYTIPKGWTVLIWTRAIHMDPQLYSNPEEFDP 415

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERVN 252
           SRW         +N++PKPG ++PFGIG+RFCPGSELTKL +TILLHHFIL NYKMERVN
Sbjct: 416 SRW---------ENHSPKPGTFIPFGIGTRFCPGSELTKLELTILLHHFIL-NYKMERVN 475

Query: 253 SRCDTTHLPAPKLVDNCLCRIIKLP 278
            +C  THLPAPKLVDNCLC I KLP
Sbjct: 476 PKCCITHLPAPKLVDNCLCTITKLP 486

BLAST of MELO3C030350 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.2e-64
Identity = 127/264 (48.11%), Postives = 175/264 (66.29%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           ARK++  I+  V++E+R ++E   +   Q     +D L+   DENG++L D + I DL +
Sbjct: 239 ARKKLAKIVQPVVDERRLMIENGPQEGSQRKDL-IDILLEVKDENGRKLEDED-ISDLLI 298

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
           GLL AGH + A + MW +  ++Q+ H+ +KAKEEQE I + R SSQK LS KEIKQM YL
Sbjct: 299 GLLFAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYL 358

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
            + ++E LR   IA A+FRE T +V IN Y IPKGW ++IW RA+HMD + Y NP+EF+P
Sbjct: 359 SQVIDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNP 418

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERVN 252
           SRW+         +Y  K G +LPFG GSR CPG++L KL I+I LH+F L NY++ER+N
Sbjct: 419 SRWD---------DYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYF-LRNYRLERIN 478

Query: 253 SRCDTTHLPAPKLVDNCLCRIIKL 277
             C  T LP  K  DNCL ++IK+
Sbjct: 479 PECHVTSLPVSKPTDNCLAKVIKV 490

BLAST of MELO3C030350 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.3e-58
Identity = 113/264 (42.80%), Postives = 172/264 (65.15%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           AR+ +  +L  V++E+R    R K      V   MD+LI A DE G+ L D++ IID+ +
Sbjct: 268 ARRRLVAVLQGVLDERRAA--RAKGVSGGGVD-MMDRLIEAQDERGRHL-DDDEIIDVLV 327

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
             L+AGH +  +  MW  V + +N  +F +AK EQE IM+  PSSQ+GL+ ++ ++M+YL
Sbjct: 328 MYLNAGHESSGHITMWATVFLQENPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYL 387

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
            + ++E LR   I+  SFR+ T +V++N Y IPKGW + +W R+VHMDPQ+Y +P +FDP
Sbjct: 388 SQVIDETLRLVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDP 447

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERVN 252
           SRW         + ++P+ G +L FG+G+R CPG++L KL I++ LHHF+L  YK+ R N
Sbjct: 448 SRW---------EGHSPRAGTFLAFGLGARLCPGNDLAKLEISVFLHHFLL-GYKLARTN 507

Query: 253 SRCDTTHLPAPKLVDNCLCRIIKL 277
            RC   +LP P+ VDNCL +I ++
Sbjct: 508 PRCRVRYLPHPRPVDNCLAKITRV 517

BLAST of MELO3C030350 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 5.4e-57
Identity = 113/264 (42.80%), Postives = 163/264 (61.74%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           AR+ +   L  V++E RR    K           MD+LI   DE G+RL D+  IID+ +
Sbjct: 243 ARRRLVAALQRVLDE-RRARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDE-IIDVLV 302

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
             L+AGH +  +  MW  V + +N  +  KAK EQE IM+  P  QKGL+ ++ ++M YL
Sbjct: 303 MYLNAGHESSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYL 362

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
            + V+E LR   I+  SFR+ T +V++N Y IPKGW + +W R+VHMDPQ+Y +P++FDP
Sbjct: 363 SQVVDETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDP 422

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERVN 252
           SRW         +   P+ G +LPFG+G+R CPG++L KL I++ LHHF+L  YK+ R N
Sbjct: 423 SRW---------EGPPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLL-GYKLTRKN 482

Query: 253 SRCDTTHLPAPKLVDNCLCRIIKL 277
             C   +LP P+ VDNCL +I +L
Sbjct: 483 PNCRVRYLPHPRPVDNCLAKITRL 494

BLAST of MELO3C030350 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.1e-56
Identity = 109/264 (41.29%), Postives = 168/264 (63.64%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           AR+++ ++L  V++ +R      K  K       MD+LI A DE G+RL D+  I+D+ +
Sbjct: 252 ARRKLVSVLQGVLDGRRAA--AAKGFKRSGAMDMMDRLIEAEDERGRRLADDE-IVDVLI 311

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
             L+AGH +  +  MW  V + +N  +F +AK EQE IM+  P++Q GL+ ++ K+M +L
Sbjct: 312 MYLNAGHESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFL 371

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
            + V+E LR   I+  SFR+ T ++++N Y IPKGW + +W R+VHMD Q+Y +P+ F+P
Sbjct: 372 SQVVDETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNP 431

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERVN 252
           SRW         +   PK G +LPFG+G+R CPG++L KL I++ LHHF+L  YK++R N
Sbjct: 432 SRW---------EGPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLL-GYKLKRAN 491

Query: 253 SRCDTTHLPAPKLVDNCLCRIIKL 277
            +C   +LP P+ VDNCL  I K+
Sbjct: 492 PKCRVRYLPHPRPVDNCLATITKV 502

BLAST of MELO3C030350 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 6.6e-55
Identity = 110/266 (41.35%), Postives = 162/266 (60.90%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKR-----RVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAI 72
           ARK +      ++ E+R      +L  KK+         +D L+   DE+GK L D+  I
Sbjct: 240 ARKTLVAAFQSIVTERRNQRKQNILSNKKD--------MLDNLLNVKDEDGKTL-DDEEI 299

Query: 73  IDLFLGLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIK 132
           ID+ L  L+AGH +  +  MW  V + ++  V Q+AK EQE I+K RP  QKGLS KE +
Sbjct: 300 IDVLLMYLNAGHESSGHTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETR 359

Query: 133 QMKYLMKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNP 192
           +M++L + V+E LR  T ++ +FRE  T+V +N Y IPKGW ++ W R VH+DP+++ +P
Sbjct: 360 KMEFLSQVVDETLRVITFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDP 419

Query: 193 QEFDPSRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYK 252
           ++FDP+RW        +  + PK G +LPFG GS  CPG++L KL I+I LHHF+L  Y+
Sbjct: 420 RKFDPARW--------DNGFVPKAGAFLPFGAGSHLCPGNDLAKLEISIFLHHFLL-KYQ 479

Query: 253 MERVNSRCDTTHLPAPKLVDNCLCRI 274
           ++R N  C   +LP  +  DNCL RI
Sbjct: 480 VKRSNPECPVMYLPHTRPTDNCLARI 487

BLAST of MELO3C030350 vs. ExPASy TrEMBL
Match: A0A1S4E5L3 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103503515 PE=3 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 1.0e-114
Identity = 210/237 (88.61%), Postives = 218/237 (91.98%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           ARKEIGNIL+CVIEEKRRVLER KETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDL L
Sbjct: 214 ARKEIGNILECVIEEKRRVLERNKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLLL 273

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
           GLLHAGHHTPAYAA W VVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL
Sbjct: 274 GLLHAGHHTPAYAATWAVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 333

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
           MKFVNELLRRY IAVASFRE TTNVYINDYTIPKGW + IWTRA+HMDPQ+YSNPQEFDP
Sbjct: 334 MKFVNELLRRYAIAVASFREATTNVYINDYTIPKGWIVTIWTRAIHMDPQMYSNPQEFDP 393

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKME 250
           SRW+         NYTPKPGE++PFG+GSRFCPGSELTKL ITILLHHFIL NYKME
Sbjct: 394 SRWD---------NYTPKPGEFIPFGLGSRFCPGSELTKLEITILLHHFIL-NYKME 440

BLAST of MELO3C030350 vs. ExPASy TrEMBL
Match: A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 5.9e-107
Identity = 196/265 (73.96%), Postives = 223/265 (84.15%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           ARK+I NIL CVIEEKRR LE+    +++ V+ QMDKLI+A DENG +LYDNNAIIDL L
Sbjct: 236 ARKKIENILQCVIEEKRRALEK----EEREVESQMDKLIMARDENGMKLYDNNAIIDLLL 295

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
           GLLHAGHHTPAYAAMW +V IS+  HVF KAKEEQE I+K+RPS+QKGL+F+EIKQMKYL
Sbjct: 296 GLLHAGHHTPAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYL 355

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
           MKF+NELLRR TIAVASFR+   +V INDY IPKGW ++IWTRA+HMDPQ+YSNPQEFDP
Sbjct: 356 MKFINELLRRNTIAVASFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDP 415

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERVN 252
           SRW         +NY+PKPG +LPFGIG+RFCPGSELTKL ITILLHHFIL NYKMERVN
Sbjct: 416 SRW---------ENYSPKPGTFLPFGIGTRFCPGSELTKLEITILLHHFIL-NYKMERVN 475

Query: 253 SRCDTTHLPAPKLVDNCLCRIIKLP 278
            +C  THLPAPKLVDNCLC I KLP
Sbjct: 476 PKCCITHLPAPKLVDNCLCTITKLP 486

BLAST of MELO3C030350 vs. ExPASy TrEMBL
Match: A0A6J1GJP8 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454912 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.7e-106
Identity = 193/265 (72.83%), Postives = 223/265 (84.15%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           ARK+I NIL CVIEEKRR LE+    +++ V+ QMDKLI+A DENG +LYDNNAIIDL L
Sbjct: 236 ARKKIENILQCVIEEKRRALEK----QEREVESQMDKLIMARDENGMKLYDNNAIIDLLL 295

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
           GLLH GHHTPAYAAMW +V IS+  HVF KAKEEQE I+K+R S+QKGL+F+EIKQMKYL
Sbjct: 296 GLLHGGHHTPAYAAMWALVHISETPHVFHKAKEEQESILKQRSSTQKGLTFQEIKQMKYL 355

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
           MKF+NELLRR TIAVASFRE  T + INDYTIPKGWT++IWTRA+HMDPQ+YSNP+EFDP
Sbjct: 356 MKFINELLRRNTIAVASFREAKTEININDYTIPKGWTVLIWTRAIHMDPQLYSNPEEFDP 415

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERVN 252
           SRW         +N++PKPG ++PFGIG+RFCPGSELTKL +TILLHHFIL NYKMERVN
Sbjct: 416 SRW---------ENHSPKPGTFIPFGIGTRFCPGSELTKLELTILLHHFIL-NYKMERVN 475

Query: 253 SRCDTTHLPAPKLVDNCLCRIIKLP 278
            +C  THLPAPKLVDNCLC I KLP
Sbjct: 476 PKCCITHLPAPKLVDNCLCTITKLP 486

BLAST of MELO3C030350 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 2.5e-105
Identity = 191/265 (72.08%), Postives = 224/265 (84.53%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           ARK+I N+L+CVIEEKRR LE +KET++  V  QMDKLI+A DENG +LYDNN IIDL L
Sbjct: 236 ARKKIENVLECVIEEKRRALE-QKETRE--VHSQMDKLIMARDENGMKLYDNNTIIDLLL 295

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
           GLLHAGHHTPAYAAMW +V IS+  H+F KAKEEQE I+K+RPS+QKGL+F+EIKQMKYL
Sbjct: 296 GLLHAGHHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYL 355

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
           MKF+NELLRR TI VASFR+  T++ INDY IPKGW+++IWTRAVHMDPQ+YSNP+EFDP
Sbjct: 356 MKFINELLRRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDP 415

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERVN 252
           SRW         +N++PKPG ++PFGIG+RFCPGSELTKL ITILLHHFIL NYKMER N
Sbjct: 416 SRW---------ENHSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFIL-NYKMERTN 475

Query: 253 SRCDTTHLPAPKLVDNCLCRIIKLP 278
            +C  +HLPAPKLVDNCLC I KLP
Sbjct: 476 PKCCISHLPAPKLVDNCLCTITKLP 487

BLAST of MELO3C030350 vs. ExPASy TrEMBL
Match: A0A6J1I8I5 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470631 PE=3 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 1.2e-104
Identity = 193/274 (70.44%), Postives = 225/274 (82.12%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           ARK+I NIL CVIEEKRR LE+    +++ V+ QMDKLI+A DENG +LYDNNAIIDL L
Sbjct: 236 ARKKIENILQCVIEEKRRALEK----QEREVESQMDKLIMARDENGMKLYDNNAIIDLLL 295

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
           GLLH GHHTPAYAAMW +VQI +  HVF KAKEEQE I+K+RPS+QKGL+F+EIKQMKYL
Sbjct: 296 GLLHGGHHTPAYAAMWALVQIFETPHVFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYL 355

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYIN---------DYTIPKGWTIMIWTRAVHMDPQI 192
           MKF+NELLRR TIAVASFRE  T++ IN         DYTIPKGWT++IWTRA+HMDPQ+
Sbjct: 356 MKFINELLRRNTIAVASFREAKTDININGELFYYIVLDYTIPKGWTVLIWTRAIHMDPQL 415

Query: 193 YSNPQEFDPSRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFIL 252
           YSNP+EFDPSRW         +N++PKPG ++PFG+G+RFCPGSELTKL +TILLHHFIL
Sbjct: 416 YSNPKEFDPSRW---------ENHSPKPGTFIPFGMGTRFCPGSELTKLELTILLHHFIL 475

Query: 253 NNYKMERVNSRCDTTHLPAPKLVDNCLCRIIKLP 278
            NYKMERVN +C  THLPAPKLVDNCLC I KLP
Sbjct: 476 -NYKMERVNPKCCITHLPAPKLVDNCLCTITKLP 495

BLAST of MELO3C030350 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 215.7 bits (548), Expect = 4.7e-56
Identity = 110/266 (41.35%), Postives = 162/266 (60.90%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKR-----RVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAI 72
           ARK +      ++ E+R      +L  KK+         +D L+   DE+GK L D+  I
Sbjct: 240 ARKTLVAAFQSIVTERRNQRKQNILSNKKD--------MLDNLLNVKDEDGKTL-DDEEI 299

Query: 73  IDLFLGLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIK 132
           ID+ L  L+AGH +  +  MW  V + ++  V Q+AK EQE I+K RP  QKGLS KE +
Sbjct: 300 IDVLLMYLNAGHESSGHTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETR 359

Query: 133 QMKYLMKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNP 192
           +M++L + V+E LR  T ++ +FRE  T+V +N Y IPKGW ++ W R VH+DP+++ +P
Sbjct: 360 KMEFLSQVVDETLRVITFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDP 419

Query: 193 QEFDPSRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYK 252
           ++FDP+RW        +  + PK G +LPFG GS  CPG++L KL I+I LHHF+L  Y+
Sbjct: 420 RKFDPARW--------DNGFVPKAGAFLPFGAGSHLCPGNDLAKLEISIFLHHFLL-KYQ 479

Query: 253 MERVNSRCDTTHLPAPKLVDNCLCRI 274
           ++R N  C   +LP  +  DNCL RI
Sbjct: 480 VKRSNPECPVMYLPHTRPTDNCLARI 487

BLAST of MELO3C030350 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 211.1 bits (536), Expect = 1.2e-54
Identity = 110/263 (41.83%), Postives = 163/263 (61.98%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           ARK++      ++  +R    ++K+      +  +D LI   DENG R+ D+  IIDL L
Sbjct: 239 ARKKLVAAFQSIVTNRR---NQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLL 298

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
             L+AGH +  +  MW  + + ++  + QKAKEEQE I+K+R   QK L+ KE ++M YL
Sbjct: 299 MYLNAGHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYL 358

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
            + ++E LR  T ++ +FRE  ++V ++ Y IPKGW ++ W R VH+DP+IY +P++FDP
Sbjct: 359 SQVIDETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDP 418

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERVN 252
           SRW         + YTPK G +LPFG+GS  CPG++L KL I+I LHHF+L  Y++ER N
Sbjct: 419 SRW---------EGYTPKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSN 478

Query: 253 SRCDTTHLPAPKLVDNCLCRIIK 276
             C    LP  +  DNCL RI +
Sbjct: 479 PGCPVMFLPHNRPKDNCLARITR 486

BLAST of MELO3C030350 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 211.1 bits (536), Expect = 1.2e-54
Identity = 110/263 (41.83%), Postives = 163/263 (61.98%), Query Frame = 0

Query: 13  ARKEIGNILDCVIEEKRRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFL 72
           ARK++      ++  +R    ++K+      +  +D LI   DENG R+ D+  IIDL L
Sbjct: 239 ARKKLVAAFQSIVTNRR---NQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLL 298

Query: 73  GLLHAGHHTPAYAAMWGVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYL 132
             L+AGH +  +  MW  + + ++  + QKAKEEQE I+K+R   QK L+ KE ++M YL
Sbjct: 299 MYLNAGHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYL 358

Query: 133 MKFVNELLRRYTIAVASFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDP 192
            + ++E LR  T ++ +FRE  ++V ++ Y IPKGW ++ W R VH+DP+IY +P++FDP
Sbjct: 359 SQVIDETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDP 418

Query: 193 SRWNFIFVKCDEQNYTPKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERVN 252
           SRW         + YTPK G +LPFG+GS  CPG++L KL I+I LHHF+L  Y++ER N
Sbjct: 419 SRW---------EGYTPKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSN 478

Query: 253 SRCDTTHLPAPKLVDNCLCRIIK 276
             C    LP  +  DNCL RI +
Sbjct: 479 PGCPVMFLPHNRPKDNCLARITR 486

BLAST of MELO3C030350 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 124.8 bits (312), Expect = 1.1e-28
Identity = 71/223 (31.84%), Postives = 115/223 (51.57%), Query Frame = 0

Query: 29  RRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFLGLLHAGHHTPAYAAMW 88
           R +L+ ++E   +      D +I  I + G  L +  A +DL   LL A   T + A   
Sbjct: 233 RNMLQERRENPRKNPSDFFDYVIEEIQKEGTILTEEIA-LDLMFVLLFASFETTSLALTL 292

Query: 89  GVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYLMKFVNELLRRYTIAVA 148
            +  +S +  V ++  EE E I++ R  +  GL+++E K M Y  +F+NE  R   I  A
Sbjct: 293 AIKFLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPA 352

Query: 149 SFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDPSRWNFIFVKCDEQNYT 208
            FR+   ++   DYTIP GW +M+   AVH++P++Y +P  F+PSRW       +    T
Sbjct: 353 IFRKALRDIKFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRW-------EGSKVT 412

Query: 209 PKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERV 252
                ++ FG G RFC G++ TKL +   LH  ++  Y+ E +
Sbjct: 413 NASKHFMAFGGGMRFCVGTDFTKLQMAAFLHS-LVTKYRWEEI 446

BLAST of MELO3C030350 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 124.8 bits (312), Expect = 1.1e-28
Identity = 71/223 (31.84%), Postives = 115/223 (51.57%), Query Frame = 0

Query: 29  RRVLERKKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLFLGLLHAGHHTPAYAAMW 88
           R +L+ ++E   +      D +I  I + G  L +  A +DL   LL A   T + A   
Sbjct: 239 RNMLQERRENPRKNPSDFFDYVIEEIQKEGTILTEEIA-LDLMFVLLFASFETTSLALTL 298

Query: 89  GVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYLMKFVNELLRRYTIAVA 148
            +  +S +  V ++  EE E I++ R  +  GL+++E K M Y  +F+NE  R   I  A
Sbjct: 299 AIKFLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPA 358

Query: 149 SFRETTTNVYINDYTIPKGWTIMIWTRAVHMDPQIYSNPQEFDPSRWNFIFVKCDEQNYT 208
            FR+   ++   DYTIP GW +M+   AVH++P++Y +P  F+PSRW       +    T
Sbjct: 359 IFRKALRDIKFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRW-------EGSKVT 418

Query: 209 PKPGEYLPFGIGSRFCPGSELTKLYITILLHHFILNNYKMERV 252
                ++ FG G RFC G++ TKL +   LH  ++  Y+ E +
Sbjct: 419 NASKHFMAFGGGMRFCVGTDFTKLQMAAFLHS-LVTKYRWEEI 452