MELO3C029194 (gene) Melon (DHL92) v4

Overview
NameMELO3C029194
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationchr01: 28127212 .. 28128755 (-)
RNA-Seq ExpressionMELO3C029194
SyntenyMELO3C029194
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGCAGTAATATCAACCTCCTCGATACCCGTTATTAGTTTGTTAGAATTCCAAAGAAAAAATGGAGTGGAAAATTCGATCTCAAGGTTGATAAAAACGTACTTTATGTCAATTGAATTTGAGTTAACTTTTATTTTAACTATTAGTTTGTATGGGTACAAAACAATTTGTATTATAAACCTACAGCAGTACTGATAAACTAACATTAAAATGATGATTAATAATTGTTTTTTTAGGGAGGTGGTGAAAGCATATCTATTGAAGATAGAAAAGTTTGCATTAGAGATATTAGAGGTGTTAAGTGAAGGGTTAGGACTTGAGAAAGGGTATTTGAGAGGTGAAATGAGTAAAAATCCAGAGTTGTTTGTACACCATTACCCACCATGCCCAAACCCTAGCTTGACTATAGGGTTGAACCAACATGCTGATCCAACACTCATCACCATCTTGTTTCAAGATGTCAATGGCCTTCAATTCCTCAAAGATGGTCAATGGATTGATGTTGATCCTCTTCCTAATGCCTTTCTCGTTAATCTTGGCTATGTTCTTGAGGTACGTACGCTTTCCAAACACTGTAAAAAAAATGACTTTACATGACATTTTTAAATATTAAATAACATATATGCATTTTAAAATATGATAATTAATTTAAATAACAAAATTGTTTGAAAATGTTATCAAATATAGCAAAATGACACTATCGTCTGTCGTATATATATACAACGACTTTTTGTTATATATTTATAAATAAGTTAGCTCATTTTTTCTTATATTTAAAAATTGTCCAACAAGATGACATAAGTATATGTAATAATGCAATTTTGAATTACTTCTCAGCTTTTGCATTTAATATAAAAATTATAGAAATGAGGGACTGAACCTCCCACCTCTAAAATGGAAAGTCATACCAACTACTACTTAACTAAGCTAACTTTGACATTTGTGGTTTTTATATGTAGGTGATAAGCAATGGGAAGATGAAAGCAGTGGAACATCGAGTTGTGACAAATACTACAACATCTCGACAAAGCTTGGCGTATGTAGTTTATCCTGGAAATGATGTGAATATAGAACCATCAAAATGTTTCATAAATGAAGCCAACCCTCCACATTATCTCTCCCTCGAGTTCAAAGATTTCATCAAGAAACTTATGTCTAAGCCTCCTGACAGGGAGACAGCATTGAAGTTTATTAGCTTCAACCAACCTTAGTGGGTTACCCAAAGTAAAATTGTCACGTTACTTTGGTGAATTATATTACTATATTTTGTAATGTATTAGATGAACTATTTTGTTAGTATGAAAAGTCAGGTCATTATATATTATTGTAATGTACTACAAAAATAACAACTTATGGCATCACTACACTATCCTTATGTAAGAAAAAAGTTGTAAGAAACGTCATACATGCTATCATATAAATAAAAAGAAAAGTTCTAATATCTCTACACCCTTGACTTTAATTCAAGGAGTAGTGGGTTTAACTTTTACTGTCTTATTAAAACTTGATAATTATTAATTGTAAAATTTTCTAACTTCGAC

mRNA sequence

ATGTCTGCAGTAATATCAACCTCCTCGATACCCGTTATTAGTTTGTTAGAATTCCAAAGAAAAAATGGAGTGGAAAATTCGATCTCAAGGGAGGTGGTGAAAGCATATCTATTGAAGATAGAAAAGTTTGCATTAGAGATATTAGAGGTGTTAAGTGAAGGGTTAGGACTTGAGAAAGGGTATTTGAGAGGTGAAATGAGTAAAAATCCAGAGTTGTTTGTACACCATTACCCACCATGCCCAAACCCTAGCTTGACTATAGGGTTGAACCAACATGCTGATCCAACACTCATCACCATCTTGTTTCAAGATGTCAATGGCCTTCAATTCCTCAAAGATGGTCAATGGATTGATGTTGATCCTCTTCCTAATGCCTTTCTCGTTAATCTTGGCTATGTTCTTGAGGTGATAAGCAATGGGAAGATGAAAGCAGTGGAACATCGAGTTGTGACAAATACTACAACATCTCGACAAAGCTTGGCGTATGTAGTTTATCCTGGAAATGATGTGAATATAGAACCATCAAAATGTTTCATAAATGAAGCCAACCCTCCACATTATCTCTCCCTCGAGTTCAAAGATTTCATCAAGAAACTTATGTCTAAGCCTCCTGACAGGGAGACAGCATTGAAGTTTATTAGCTTCAACCAACCTTAGTGGGTTACCCAAAGTAAAATTGTCACGTTACTTTGGTGAATTATATTACTATATTTTGTAATGTATTAGATGAACTATTTTGTTAGTATGAAAAGTCAGGTCATTATATATTATTGTAATGTACTACAAAAATAACAACTTATGGCATCACTACACTATCCTTATGTAAGAAAAAAGTTGTAAGAAACGTCATACATGCTATCATATAAATAAAAAGAAAAGTTCTAATATCTCTACACCCTTGACTTTAATTCAAGGAGTAGTGGGTTTAACTTTTACTGTCTTATTAAAACTTGATAATTATTAATTGTAAAATTTTCTAACTTCGAC

Coding sequence (CDS)

ATGTCTGCAGTAATATCAACCTCCTCGATACCCGTTATTAGTTTGTTAGAATTCCAAAGAAAAAATGGAGTGGAAAATTCGATCTCAAGGGAGGTGGTGAAAGCATATCTATTGAAGATAGAAAAGTTTGCATTAGAGATATTAGAGGTGTTAAGTGAAGGGTTAGGACTTGAGAAAGGGTATTTGAGAGGTGAAATGAGTAAAAATCCAGAGTTGTTTGTACACCATTACCCACCATGCCCAAACCCTAGCTTGACTATAGGGTTGAACCAACATGCTGATCCAACACTCATCACCATCTTGTTTCAAGATGTCAATGGCCTTCAATTCCTCAAAGATGGTCAATGGATTGATGTTGATCCTCTTCCTAATGCCTTTCTCGTTAATCTTGGCTATGTTCTTGAGGTGATAAGCAATGGGAAGATGAAAGCAGTGGAACATCGAGTTGTGACAAATACTACAACATCTCGACAAAGCTTGGCGTATGTAGTTTATCCTGGAAATGATGTGAATATAGAACCATCAAAATGTTTCATAAATGAAGCCAACCCTCCACATTATCTCTCCCTCGAGTTCAAAGATTTCATCAAGAAACTTATGTCTAAGCCTCCTGACAGGGAGACAGCATTGAAGTTTATTAGCTTCAACCAACCTTAG

Protein sequence

MSAVISTSSIPVISLLEFQRKNGVENSISREVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETALKFISFNQP
Homology
BLAST of MELO3C029194 vs. NCBI nr
Match: XP_008451257.1 (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])

HSP 1 Score: 387.5 bits (994), Expect = 7.6e-104
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 89
           REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL
Sbjct: 151 REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 210

Query: 90  NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 149
           NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV
Sbjct: 211 NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 270

Query: 150 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 209
           VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA
Sbjct: 271 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 330

Query: 210 LKFISFNQP 219
           LKFISFNQP
Sbjct: 331 LKFISFNQP 339

BLAST of MELO3C029194 vs. NCBI nr
Match: KAA0059649.1 (hyoscyamine 6-dioxygenase-like [Cucumis melo var. makuwa] >TYK08181.1 hyoscyamine 6-dioxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 387.5 bits (994), Expect = 7.6e-104
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 89
           REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL
Sbjct: 151 REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 210

Query: 90  NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 149
           NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV
Sbjct: 211 NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 270

Query: 150 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 209
           VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA
Sbjct: 271 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 330

Query: 210 LKFISFNQP 219
           LKFISFNQP
Sbjct: 331 LKFISFNQP 339

BLAST of MELO3C029194 vs. NCBI nr
Match: XP_004148957.1 (hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44743.1 hypothetical protein Csa_016242 [Cucumis sativus])

HSP 1 Score: 341.3 bits (874), Expect = 6.3e-90
Identity = 162/188 (86.17%), Postives = 174/188 (92.55%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 89
           REVVKAY++ IEKFA EILEVLSEGLGLEKGY RGEMSKNPE   HHYPPCPNPSLT+GL
Sbjct: 149 REVVKAYIVMIEKFASEILEVLSEGLGLEKGYFRGEMSKNPEFLAHHYPPCPNPSLTVGL 208

Query: 90  NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 149
           +QHADPTLITILFQDVNGLQFLKDGQWIDVDPLP+AFLVNLGYV EVISNGK+KA EHRV
Sbjct: 209 SQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPDAFLVNLGYVFEVISNGKLKAAEHRV 268

Query: 150 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 209
           VTN+TTSRQ+L YVVYP NDV IEPSKCFINEANPPHY SLEFKDF  KLMSKPPDR+TA
Sbjct: 269 VTNSTTSRQTLVYVVYPENDVTIEPSKCFINEANPPHYRSLEFKDFYNKLMSKPPDRKTA 328

Query: 210 LKFISFNQ 218
           ++FISF+Q
Sbjct: 329 MEFISFDQ 336

BLAST of MELO3C029194 vs. NCBI nr
Match: XP_011659768.2 (protein DOWNY MILDEW RESISTANCE 6 [Cucumis sativus] >KAE8646291.1 hypothetical protein Csa_015678 [Cucumis sativus])

HSP 1 Score: 326.2 bits (835), Expect = 2.1e-85
Identity = 155/189 (82.01%), Postives = 171/189 (90.48%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 89
           REV++AYL+ ++KF+LEILEVLSEGLGLEK Y  G MS NPE  VHHYPPCPNPSLT+GL
Sbjct: 150 REVMEAYLVVMKKFSLEILEVLSEGLGLEKEYFSGGMSNNPEFLVHHYPPCPNPSLTLGL 209

Query: 90  NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 149
           N+HADPT+ITILFQDVNGLQFLKDG+WIDVDPLP+AFLVNLGYV EVISNGKMKA EHRV
Sbjct: 210 NKHADPTVITILFQDVNGLQFLKDGEWIDVDPLPDAFLVNLGYVFEVISNGKMKAAEHRV 269

Query: 150 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 209
           VTNTTTSRQSLAY VYP N++ IEPSKCFINEANPPHY +LEFKDF+ KL SK PDRE A
Sbjct: 270 VTNTTTSRQSLAYGVYPENNMTIEPSKCFINEANPPHYRTLEFKDFVNKLRSKHPDREMA 329

Query: 210 LKFISFNQP 219
           +KFISFNQP
Sbjct: 330 MKFISFNQP 338

BLAST of MELO3C029194 vs. NCBI nr
Match: KAA0059663.1 (hyoscyamine 6-dioxygenase-like [Cucumis melo var. makuwa] >TYK26103.1 hyoscyamine 6-dioxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 260.8 bits (665), Expect = 1.1e-65
Identity = 120/188 (63.83%), Postives = 151/188 (80.32%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 89
           REVV AY + + K  LEILE++SEGLGL KGYL GEMS+NP L V+HYPPCPNPSLT+GL
Sbjct: 70  REVVGAYCVAMRKLVLEILELMSEGLGLGKGYLGGEMSENPLLLVNHYPPCPNPSLTLGL 129

Query: 90  NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 149
           +QH DP+LITILFQDVNGLQ LKDGQWI V P+ NAF+VN+G+VL++I+NGK++A EHR 
Sbjct: 130 SQHCDPSLITILFQDVNGLQVLKDGQWIGVQPIDNAFVVNIGFVLQIITNGKLQAAEHRA 189

Query: 150 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 209
           VTN+ TSRQSL Y+VYP ++  +EP+KC INEANPP Y SL FKDF +  + +  D +  
Sbjct: 190 VTNSKTSRQSLTYLVYPKDEATMEPAKCMINEANPPRYRSLNFKDFQRNYLPRAVDTKAV 249

Query: 210 LKFISFNQ 218
           +++I  NQ
Sbjct: 250 MQYIETNQ 257

BLAST of MELO3C029194 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.4e-36
Identity = 78/189 (41.27%), Postives = 114/189 (60.32%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 89
           REVV  Y +++ K  + +L+ + EGLGL+ GY   E+S+   +  ++YPPCP+PS T+G 
Sbjct: 155 REVVAKYSVEVRKLTMRMLDYICEGLGLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGS 214

Query: 90  NQHADPTLITILFQDVNGLQ--FLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEH 149
             H D  LIT+L QD+ GLQ   +KD  WI V P+P AF+VNLG  L+VI+N K +   H
Sbjct: 215 GGHYDGNLITLLQQDLPGLQQLIVKDATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIH 274

Query: 150 RVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRE 209
           RVVT+ T  R S+A ++ P     IEP+K  +N+ NPP Y    + +F    +S   D +
Sbjct: 275 RVVTDPTRDRVSIATLIGPDYSCTIEPAKELLNQDNPPLYKPYSYSEFADIYLSDKSDYD 334

Query: 210 TALKFISFN 217
           + +K    N
Sbjct: 335 SGVKPYKIN 343

BLAST of MELO3C029194 vs. ExPASy Swiss-Prot
Match: P31239 (1-aminocyclopropane-1-carboxylate oxidase OS=Pisum sativum OX=3888 GN=ACO PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.5e-35
Identity = 86/197 (43.65%), Postives = 116/197 (58.88%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLR----GEMSKNPELFVHHYPPCPNPSL 89
           R+V+K + LK+E+ A E+L++L E LGLEKGYL+    G    N    V +YPPCP P L
Sbjct: 111 RKVMKEFALKLEELAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPEL 170

Query: 90  TIGLNQHADPTLITILFQD--VNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMK 149
             GL  H D   I +LFQD  V+GLQ LKD QWIDV P+ ++ ++NLG  LEVI+NGK K
Sbjct: 171 IKGLRAHTDAGGIILLFQDDKVSGLQLLKDDQWIDVPPMRHSIVINLGDQLEVITNGKYK 230

Query: 150 AVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPH-YLSLEFKDFIK----- 209
           +V HRV+  T  +R S+A    PG+D  I P+   + E      Y    F D++K     
Sbjct: 231 SVMHRVIAQTDGARMSIASFYNPGDDAVISPASTLLKENETSEVYPKFVFDDYMKLYMGL 290

Query: 210 KLMSKPPDRETALKFIS 215
           K  +K P  E  +K +S
Sbjct: 291 KFQAKEPRFEAMMKAMS 307

BLAST of MELO3C029194 vs. ExPASy Swiss-Prot
Match: Q9MB94 (1-aminocyclopropane-1-carboxylate oxidase OS=Prunus mume OX=102107 GN=ACO1 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 7.8e-35
Identity = 88/201 (43.78%), Postives = 119/201 (59.20%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEM-SKNPELF---VHHYPPCPNPSL 89
           R V+K + LK+EK A ++L++L E LGLEKGYL+      N   F   V +YPPCPNP L
Sbjct: 111 RNVMKEFALKLEKLAEQLLDLLCENLGLEKGYLKKAFYGTNGPTFGTKVSNYPPCPNPEL 170

Query: 90  TIGLNQHADPTLITILFQD--VNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMK 149
             GL  H D   + +LFQD  V+GLQ LKDGQWIDV P+ ++ ++NLG  LEVI+NGK +
Sbjct: 171 IKGLRAHTDAGGLILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVINLGDQLEVITNGKYR 230

Query: 150 AVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFIN---EANPPHYLSLEFKDFIK--- 209
           +VEHRV+  T  +R S+A    PG+D  I P+   +    E     Y    F+D++K   
Sbjct: 231 SVEHRVIAQTDGTRMSIASFYNPGSDAVIYPAPTLVEKEAEEKNQVYPKFVFEDYMKLYA 290

Query: 210 --KLMSKPPDRETALKFISFN 217
             K   K P R  A+K +  N
Sbjct: 291 GLKFQPKEP-RFEAMKAVETN 310

BLAST of MELO3C029194 vs. ExPASy Swiss-Prot
Match: P24157 (1-aminocyclopropane-1-carboxylate oxidase 4 OS=Solanum lycopersicum OX=4081 GN=ACO4 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.0e-34
Identity = 91/198 (45.96%), Postives = 117/198 (59.09%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEM--SKNPEL--FVHHYPPCPNPSL 89
           REV++ +  ++EK A E+L++L E LGLEKGYL+     SK P     V +YPPCP P L
Sbjct: 111 REVMRDFAKRLEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDL 170

Query: 90  TIGLNQHADPTLITILFQD--VNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMK 149
             GL  H D   I +LFQD  V+GLQ LKD QWIDV P+ ++ +VNLG  LEVI+NGK K
Sbjct: 171 IKGLRAHTDAGGIILLFQDDKVSGLQLLKDEQWIDVPPMRHSIVVNLGDQLEVITNGKYK 230

Query: 150 AVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIK-----K 209
           +V HRV+  T  +R SLA    PGND  I P+   I E+    Y    F D++K     K
Sbjct: 231 SVMHRVIAQTDGTRMSLASFYNPGNDAVIYPAPSLIEESKQV-YPKFVFDDYMKLYAGLK 290

Query: 210 LMSKPPDRETALKFISFN 217
              K P R  A+K +  N
Sbjct: 291 FQPKEP-RFEAMKAMEAN 306

BLAST of MELO3C029194 vs. ExPASy Swiss-Prot
Match: Q43792 (1-aminocyclopropane-1-carboxylate oxidase OS=Nicotiana tabacum OX=4097 GN=ACO PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.7e-34
Identity = 88/196 (44.90%), Postives = 114/196 (58.16%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLR----GEMSKNPELFVHHYPPCPNPSL 89
           REV++ +  ++EK A E+L++L E LGLEKGYL+    G    N    V +YPPCP P L
Sbjct: 111 REVMRDFAKRLEKLAEELLDLLCENLGLEKGYLKKIFYGTKGPNFGSKVSNYPPCPKPDL 170

Query: 90  TIGLNQHADPTLITILFQD--VNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMK 149
             GL  H D   I +LFQD  V GLQ LKDGQWIDV P+  + +VNLG  LEVI+NGK K
Sbjct: 171 IKGLRAHTDAGGIILLFQDDKVTGLQLLKDGQWIDVPPMRLSIVVNLGDQLEVITNGKYK 230

Query: 150 AVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFIN---EANPPHYLSLEFKDFIK--- 209
           +V HRV+T T  +R SLA    PG+D  I P+   +    E +   Y    F D++K   
Sbjct: 231 SVMHRVITQTDGTRMSLASFYNPGSDAVIFPAPTLVEKEAEESKAIYPKFVFDDYMKLYA 290

Query: 210 --KLMSKPPDRETALK 212
             K  +K P  E  +K
Sbjct: 291 GLKFQAKEPRFEAMIK 306

BLAST of MELO3C029194 vs. ExPASy TrEMBL
Match: A0A5A7V1J0 (Hyoscyamine 6-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00760 PE=3 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 3.7e-104
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 89
           REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL
Sbjct: 151 REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 210

Query: 90  NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 149
           NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV
Sbjct: 211 NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 270

Query: 150 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 209
           VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA
Sbjct: 271 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 330

Query: 210 LKFISFNQP 219
           LKFISFNQP
Sbjct: 331 LKFISFNQP 339

BLAST of MELO3C029194 vs. ExPASy TrEMBL
Match: A0A1S3BRU2 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492607 PE=3 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 3.7e-104
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 89
           REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL
Sbjct: 151 REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 210

Query: 90  NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 149
           NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV
Sbjct: 211 NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 270

Query: 150 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 209
           VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA
Sbjct: 271 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 330

Query: 210 LKFISFNQP 219
           LKFISFNQP
Sbjct: 331 LKFISFNQP 339

BLAST of MELO3C029194 vs. ExPASy TrEMBL
Match: A0A0A0K5H0 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375850 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 3.0e-90
Identity = 162/188 (86.17%), Postives = 174/188 (92.55%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 89
           REVVKAY++ IEKFA EILEVLSEGLGLEKGY RGEMSKNPE   HHYPPCPNPSLT+GL
Sbjct: 149 REVVKAYIVMIEKFASEILEVLSEGLGLEKGYFRGEMSKNPEFLAHHYPPCPNPSLTVGL 208

Query: 90  NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 149
           +QHADPTLITILFQDVNGLQFLKDGQWIDVDPLP+AFLVNLGYV EVISNGK+KA EHRV
Sbjct: 209 SQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPDAFLVNLGYVFEVISNGKLKAAEHRV 268

Query: 150 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 209
           VTN+TTSRQ+L YVVYP NDV IEPSKCFINEANPPHY SLEFKDF  KLMSKPPDR+TA
Sbjct: 269 VTNSTTSRQTLVYVVYPENDVTIEPSKCFINEANPPHYRSLEFKDFYNKLMSKPPDRKTA 328

Query: 210 LKFISFNQ 218
           ++FISF+Q
Sbjct: 329 MEFISFDQ 336

BLAST of MELO3C029194 vs. ExPASy TrEMBL
Match: A0A0A0K745 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375860 PE=3 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 1.0e-85
Identity = 155/189 (82.01%), Postives = 171/189 (90.48%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 89
           REV++AYL+ ++KF+LEILEVLSEGLGLEK Y  G MS NPE  VHHYPPCPNPSLT+GL
Sbjct: 149 REVMEAYLVVMKKFSLEILEVLSEGLGLEKEYFSGGMSNNPEFLVHHYPPCPNPSLTLGL 208

Query: 90  NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 149
           N+HADPT+ITILFQDVNGLQFLKDG+WIDVDPLP+AFLVNLGYV EVISNGKMKA EHRV
Sbjct: 209 NKHADPTVITILFQDVNGLQFLKDGEWIDVDPLPDAFLVNLGYVFEVISNGKMKAAEHRV 268

Query: 150 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 209
           VTNTTTSRQSLAY VYP N++ IEPSKCFINEANPPHY +LEFKDF+ KL SK PDRE A
Sbjct: 269 VTNTTTSRQSLAYGVYPENNMTIEPSKCFINEANPPHYRTLEFKDFVNKLRSKHPDREMA 328

Query: 210 LKFISFNQP 219
           +KFISFNQP
Sbjct: 329 MKFISFNQP 337

BLAST of MELO3C029194 vs. ExPASy TrEMBL
Match: A0A5D3DR20 (Hyoscyamine 6-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00110 PE=3 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 5.2e-66
Identity = 120/188 (63.83%), Postives = 151/188 (80.32%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPELFVHHYPPCPNPSLTIGL 89
           REVV AY + + K  LEILE++SEGLGL KGYL GEMS+NP L V+HYPPCPNPSLT+GL
Sbjct: 70  REVVGAYCVAMRKLVLEILELMSEGLGLGKGYLGGEMSENPLLLVNHYPPCPNPSLTLGL 129

Query: 90  NQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEHRV 149
           +QH DP+LITILFQDVNGLQ LKDGQWI V P+ NAF+VN+G+VL++I+NGK++A EHR 
Sbjct: 130 SQHCDPSLITILFQDVNGLQVLKDGQWIGVQPIDNAFVVNIGFVLQIITNGKLQAAEHRA 189

Query: 150 VTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIKKLMSKPPDRETA 209
           VTN+ TSRQSL Y+VYP ++  +EP+KC INEANPP Y SL FKDF +  + +  D +  
Sbjct: 190 VTNSKTSRQSLTYLVYPKDEATMEPAKCMINEANPPRYRSLNFKDFQRNYLPRAVDTKAV 249

Query: 210 LKFISFNQ 218
           +++I  NQ
Sbjct: 250 MQYIETNQ 257

BLAST of MELO3C029194 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 138.7 bits (348), Expect = 5.7e-33
Identity = 68/154 (44.16%), Postives = 94/154 (61.04%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPE-LFVHHYPPCPNPSLTIG 89
           REV   Y   +    L +LE +SE LGLE  ++   + K+ + +  ++YPPCP P LT G
Sbjct: 158 REVTAEYATSVRALVLRLLEAISESLGLESDHISNILGKHAQHMAFNYYPPCPEPELTYG 217

Query: 90  LNQHADPTLITILFQD-VNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEH 149
           L  H DPT+IT+L QD V+GLQ  KD +W+ V P+PN F+VN+G  ++VISN K K+V H
Sbjct: 218 LPGHKDPTVITVLLQDQVSGLQVFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLH 277

Query: 150 RVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFINE 182
           R V NT   R S+    +P  D  I P+   +NE
Sbjct: 278 RAVVNTENERLSIPTFYFPSTDAVIGPAHELVNE 311

BLAST of MELO3C029194 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 138.7 bits (348), Expect = 5.7e-33
Identity = 74/188 (39.36%), Postives = 112/188 (59.57%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPE-LFVHHYPPCPNPSLTIG 89
           +E+V  Y  ++ +   +I E++SE LGLEK Y++  + +  + + V++YPPCP P LT G
Sbjct: 149 KEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHMAVNYYPPCPEPELTYG 208

Query: 90  LNQHADPTLITILFQD--VNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVE 149
           L  H DP  +TIL QD  V GLQ L DGQW  V+P P+AF++N+G  L+ +SNG  K+V 
Sbjct: 209 LPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINIGDQLQALSNGVYKSVW 268

Query: 150 HRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCF---INEANPPHYLSLEFKDFIKKLMSKP 209
           HR VTNT   R S+A  + P +   + P+K      ++   P Y    + ++ KK  S+ 
Sbjct: 269 HRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPVYKDFTYAEYYKKFWSRN 328

Query: 210 PDRETALK 212
            D+E  L+
Sbjct: 329 LDQEHCLE 336

BLAST of MELO3C029194 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 138.3 bits (347), Expect = 7.5e-33
Identity = 85/191 (44.50%), Postives = 115/191 (60.21%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEM--SKNPEL--FVHHYPPCPNPSL 89
           R ++K +  KIEK + E+L++L E LGLEKGYL+     SK P     V +YPPCPNP L
Sbjct: 111 RTLMKDFAGKIEKLSEELLDLLCENLGLEKGYLKKVFYGSKRPTFGTKVSNYPPCPNPDL 170

Query: 90  TIGLNQHADPTLITILFQD--VNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMK 149
             GL  H D   I +LFQD  V+GLQ LKDG+W+DV P+ ++ +VNLG  LEVI+NGK K
Sbjct: 171 VKGLRAHTDAGGIILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYK 230

Query: 150 AVEHRVVTNTT-TSRQSLAYVVYPGNDVNIEPSKCFI----NEANPPHYLSLEFKDFIK- 205
           +VEHRV++ T    R S+A    PG+D  I P+   I     +    +Y    F+D++K 
Sbjct: 231 SVEHRVLSQTDGEGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKL 290

BLAST of MELO3C029194 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 137.1 bits (344), Expect = 1.7e-32
Identity = 77/174 (44.25%), Postives = 106/174 (60.92%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEM--SKNPEL--FVHHYPPCPNPSL 89
           R  +K +  ++E  A ++L++L E LGLEKGYL+     +K P     V +YPPCP P +
Sbjct: 114 RTAMKDFGKRLENLAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEM 173

Query: 90  TIGLNQHADPTLITILFQD--VNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMK 149
             GL  H D   I +LFQD  V+GLQ LKDG WIDV PL ++ ++NLG  LEVI+NGK K
Sbjct: 174 IKGLRAHTDAGGIILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNGKYK 233

Query: 150 AVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEANPPHYLSLEFKDFIK 198
           +V HRVVT    +R S+A    PG+D  I P+   + + +   Y S  F D++K
Sbjct: 234 SVLHRVVTQQEGNRMSVASFYNPGSDAEISPATSLVEKDS--EYPSFVFDDYMK 285

BLAST of MELO3C029194 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 136.7 bits (343), Expect = 2.2e-32
Identity = 74/185 (40.00%), Postives = 106/185 (57.30%), Query Frame = 0

Query: 30  REVVKAYLLKIEKFALEILEVLSEGLGLEKGYLRGEMSKNPE-LFVHHYPPCPNPSLTIG 89
           REV   Y   +    L +LE +SE LGL K  +   + K+ + + +++YP CP P LT G
Sbjct: 156 REVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYG 215

Query: 90  LNQHADPTLITILFQD-VNGLQFLKDGQWIDVDPLPNAFLVNLGYVLEVISNGKMKAVEH 149
           L  H D  LIT+L QD V+GLQ  KDG+WI V+P+PN F+VNLG  ++VISN K K+V H
Sbjct: 216 LPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLH 275

Query: 150 RVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFINEA--NPPHYLSLEFKDFIKKLMSKPPD 209
           R V N+   R S+     P  D  I P++  INE   +P  Y +  + ++ +K      D
Sbjct: 276 RAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEKFWDTAFD 335

Query: 210 RETAL 211
            E+ +
Sbjct: 336 TESCI 340

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008451257.17.6e-104100.00PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo][more]
KAA0059649.17.6e-104100.00hyoscyamine 6-dioxygenase-like [Cucumis melo var. makuwa] >TYK08181.1 hyoscyamin... [more]
XP_004148957.16.3e-9086.17hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44743.1 hypothetical protein Csa... [more]
XP_011659768.22.1e-8582.01protein DOWNY MILDEW RESISTANCE 6 [Cucumis sativus] >KAE8646291.1 hypothetical p... [more]
KAA0059663.11.1e-6563.83hyoscyamine 6-dioxygenase-like [Cucumis melo var. makuwa] >TYK26103.1 hyoscyamin... [more]
Match NameE-valueIdentityDescription
P243971.4e-3641.27Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1[more]
P312393.5e-3543.651-aminocyclopropane-1-carboxylate oxidase OS=Pisum sativum OX=3888 GN=ACO PE=2 S... [more]
Q9MB947.8e-3543.781-aminocyclopropane-1-carboxylate oxidase OS=Prunus mume OX=102107 GN=ACO1 PE=2 ... [more]
P241571.0e-3445.961-aminocyclopropane-1-carboxylate oxidase 4 OS=Solanum lycopersicum OX=4081 GN=A... [more]
Q437921.7e-3444.901-aminocyclopropane-1-carboxylate oxidase OS=Nicotiana tabacum OX=4097 GN=ACO PE... [more]
Match NameE-valueIdentityDescription
A0A5A7V1J03.7e-104100.00Hyoscyamine 6-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BRU23.7e-104100.00hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492607 PE=3 SV=1[more]
A0A0A0K5H03.0e-9086.17Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G... [more]
A0A0A0K7451.0e-8582.01Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G... [more]
A0A5D3DR205.2e-6663.83Hyoscyamine 6-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
Match NameE-valueIdentityDescription
AT4G10500.15.7e-3344.162-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G24530.15.7e-3339.362-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G05010.17.5e-3344.50ethylene-forming enzyme [more]
AT1G62380.11.7e-3244.25ACC oxidase 2 [more]
AT4G10490.12.2e-3240.002-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 72..163
e-value: 1.6E-23
score: 83.1
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 12..214
e-value: 9.9E-57
score: 194.6
NoneNo IPR availablePANTHERPTHR47991:SF311-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASEcoord: 29..213
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 29..213
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 28..202
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 68..166
score: 13.310082

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C029194.1MELO3C029194.1mRNA