MELO3C027470 (gene) Melon (DHL92) v4

Overview


Name	MELO3C027470
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	Pyruvate kinase
Location	chr12: 18902576 .. 18902855 (-)
RNA-Seq Expression	MELO3C027470
Synteny	MELO3C027470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTCACCTTAACCAAACTCAAGTATTTGCAAAGATGGAAAGTGTAGAGGTAAGTCATTGTCAGCTCACTTTTTACTCGTATTCTTTACCATGGTTTCCTTAGGATTGATTATTTCTGTTTTCTACATTGTTACAGGTGTTTTTGTTTCAGAAAACTGCCCTTTATAGATGTAATATGGCTGGAAAGCCTGCTGTTGTAACTCGTGTGGTAGACAGCATGACTAACAACTTGAGACCTACACGTGCAGTAGCTACTGTTGTTGCCAATGCTGTACTTGATG

mRNA sequence

GTTCACCTTAACCAAACTCAAGTATTTGCAAAGATGGAAAGTGTAGAGGTGTTTTTGTTTCAGAAAACTGCCCTTTATAGATGTAATATGGCTGGAAAGCCTGCTGTTGTAACTCGTGTGGTAGACAGCATGACTAACAACTTGAGACCTACACGTGCAGTAGCTACTGTTGTTGCCAATGCTGTACTTGATG

Coding sequence (CDS)

ATGGAAAGTGTAGAGGTGTTTTTGTTTCAGAAAACTGCCCTTTATAGATGTAATATGGCTGGAAAGCCTGCTGTTGTAACTCGTGTGGTAGACAGCATGACTAACAACTTGAGACCTACACGTGCAGTAGCTACTGTTGTTGCCAATGCTGTACTTGAT

Protein sequence

MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD

Homology

[Close]

BLAST of MELO3C027470 vs. ExPASy Swiss-Prot
Match: B8BJ39 (Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_35105 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.3e-15
Identity = 42/49 (85.71%), Postives = 47/49 (95.92%), Query Frame = 0


Query: 5   EVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +VFLFQK+AL++CNMAGKPAVVTRVVDSMT+NLRPTRA AT VANAVLD
Sbjct: 291 KVFLFQKSALHKCNMAGKPAVVTRVVDSMTDNLRPTRAEATDVANAVLD 339

[Close]

BLAST of MELO3C027470 vs. ExPASy Swiss-Prot
Match: Q2RAK2 (Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0148500 PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.3e-15
Identity = 42/49 (85.71%), Postives = 47/49 (95.92%), Query Frame = 0


Query: 5   EVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +VFLFQK+AL++CNMAGKPAVVTRVVDSMT+NLRPTRA AT VANAVLD
Sbjct: 291 KVFLFQKSALHKCNMAGKPAVVTRVVDSMTDNLRPTRAEATDVANAVLD 339

[Close]

BLAST of MELO3C027470 vs. ExPASy Swiss-Prot
Match: B8BM17 (Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_37456 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.3e-15
Identity = 42/49 (85.71%), Postives = 47/49 (95.92%), Query Frame = 0


Query: 5   EVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +VFLFQK+AL++CNMAGKPAVVTRVVDSMT+NLRPTRA AT VANAVLD
Sbjct: 291 KVFLFQKSALHKCNMAGKPAVVTRVVDSMTDNLRPTRAEATDVANAVLD 339

[Close]

BLAST of MELO3C027470 vs. ExPASy Swiss-Prot
Match: Q2QXR8 (Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0145700 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.3e-15
Identity = 42/49 (85.71%), Postives = 47/49 (95.92%), Query Frame = 0


Query: 5   EVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +VFLFQK+AL++CNMAGKPAVVTRVVDSMT+NLRPTRA AT VANAVLD
Sbjct: 291 KVFLFQKSALHKCNMAGKPAVVTRVVDSMTDNLRPTRAEATDVANAVLD 339

[Close]

BLAST of MELO3C027470 vs. ExPASy Swiss-Prot
Match: Q42806 (Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 3.7e-07
Identity = 28/50 (56.00%), Postives = 38/50 (76.00%), Query Frame = 0


Query: 5   EVFLFQKTALYRCNMAGKPAV-VTRVVDSMTNNLRPTRAVATVVANAVLD 54
           ++FL QK  +Y+CN+ GKP V  T++++SM  + RPTRA AT VANAVLD
Sbjct: 276 KIFLAQKMMIYKCNLVGKPVVTATQMLESMIKSPRPTRAEATDVANAVLD 325

[Close]

BLAST of MELO3C027470 vs. NCBI nr
Match: KAA0036915.1 (pyruvate kinase 1, cytosolic-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 103.2 bits (256), Expect = 7.0e-19
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 0


Query: 1  MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
          MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD
Sbjct: 1  MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 53

[Close]

BLAST of MELO3C027470 vs. NCBI nr
Match: KAA0054856.1 (pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa])

HSP 1 Score: 96.7 bits (239), Expect = 6.5e-17
Identity = 49/53 (92.45%), Postives = 52/53 (98.11%), Query Frame = 0


Query: 1   MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +E+VEVFLFQKTALYRCNMAGKPAV+TRVVDSMTNNLRPTRAVAT VANAVLD
Sbjct: 155 IENVEVFLFQKTALYRCNMAGKPAVLTRVVDSMTNNLRPTRAVATDVANAVLD 207

[Close]

BLAST of MELO3C027470 vs. NCBI nr
Match: KAA0054848.1 (pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa])

HSP 1 Score: 95.9 bits (237), Expect = 1.1e-16
Identity = 49/53 (92.45%), Postives = 52/53 (98.11%), Query Frame = 0


Query: 1   MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +ESVEVFLFQKTALYRCNMAGKPAV+TRVV+SMTNNLRPTRAVAT VANAVLD
Sbjct: 188 IESVEVFLFQKTALYRCNMAGKPAVLTRVVNSMTNNLRPTRAVATDVANAVLD 240

[Close]

BLAST of MELO3C027470 vs. NCBI nr
Match: KAA0035250.1 (phagocyte signaling-impaired protein [Cucumis melo var. makuwa])

HSP 1 Score: 95.5 bits (236), Expect = 1.5e-16
Identity = 49/53 (92.45%), Postives = 51/53 (96.23%), Query Frame = 0


Query: 1   MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +ESVEVFLFQKTALYRCNMAGKP V+TRVVDSMTNNLRPTRAVAT VANAVLD
Sbjct: 183 IESVEVFLFQKTALYRCNMAGKPDVLTRVVDSMTNNLRPTRAVATDVANAVLD 235

[Close]

BLAST of MELO3C027470 vs. NCBI nr
Match: KAA0046425.1 (pyruvate kinase 1, cytosolic-like [Cucumis melo var. makuwa])

HSP 1 Score: 93.6 bits (231), Expect = 5.5e-16
Identity = 48/53 (90.57%), Postives = 51/53 (96.23%), Query Frame = 0


Query: 1   MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +ESVEVFL QKTALYRCNMAGKPAV+TRVVDSMTNNLRPTRAVAT VANAVL+
Sbjct: 52  IESVEVFLLQKTALYRCNMAGKPAVLTRVVDSMTNNLRPTRAVATDVANAVLN 104

[Close]

BLAST of MELO3C027470 vs. ExPASy TrEMBL
Match: A0A5A7T5Z6 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold9475G00070 PE=3 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.4e-19
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 0


Query: 1  MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
          MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD
Sbjct: 1  MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 53

[Close]

BLAST of MELO3C027470 vs. ExPASy TrEMBL
Match: A0A5A7UKP0 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold406G00640 PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.2e-17
Identity = 49/53 (92.45%), Postives = 52/53 (98.11%), Query Frame = 0


Query: 1   MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +E+VEVFLFQKTALYRCNMAGKPAV+TRVVDSMTNNLRPTRAVAT VANAVLD
Sbjct: 155 IENVEVFLFQKTALYRCNMAGKPAVLTRVVDSMTNNLRPTRAVATDVANAVLD 207

[Close]

BLAST of MELO3C027470 vs. ExPASy TrEMBL
Match: A0A5A7UIR4 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold406G00540 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 5.4e-17
Identity = 49/53 (92.45%), Postives = 52/53 (98.11%), Query Frame = 0


Query: 1   MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +ESVEVFLFQKTALYRCNMAGKPAV+TRVV+SMTNNLRPTRAVAT VANAVLD
Sbjct: 188 IESVEVFLFQKTALYRCNMAGKPAVLTRVVNSMTNNLRPTRAVATDVANAVLD 240

[Close]

BLAST of MELO3C027470 vs. ExPASy TrEMBL
Match: A0A5A7SW39 (Phagocyte signaling-impaired protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold228G00330 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 7.0e-17
Identity = 49/53 (92.45%), Postives = 51/53 (96.23%), Query Frame = 0


Query: 1   MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +ESVEVFLFQKTALYRCNMAGKP V+TRVVDSMTNNLRPTRAVAT VANAVLD
Sbjct: 183 IESVEVFLFQKTALYRCNMAGKPDVLTRVVDSMTNNLRPTRAVATDVANAVLD 235

[Close]

BLAST of MELO3C027470 vs. ExPASy TrEMBL
Match: A0A5A7TYG6 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold149G001810 PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.7e-16
Identity = 48/53 (90.57%), Postives = 51/53 (96.23%), Query Frame = 0


Query: 1   MESVEVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +ESVEVFL QKTALYRCNMAGKPAV+TRVVDSMTNNLRPTRAVAT VANAVL+
Sbjct: 52  IESVEVFLLQKTALYRCNMAGKPAVLTRVVDSMTNNLRPTRAVATDVANAVLN 104

[Close]

BLAST of MELO3C027470 vs. TAIR 10
Match: AT2G36580.1 (Pyruvate kinase family protein )

HSP 1 Score: 83.2 bits (204), Expect = 7.0e-17
Identity = 42/49 (85.71%), Postives = 46/49 (93.88%), Query Frame = 0


Query: 5   EVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +VFLFQK ALY+CNMAGKPAV+TRVVDSMT+NLRPTRA AT VANAVLD
Sbjct: 291 KVFLFQKAALYKCNMAGKPAVLTRVVDSMTDNLRPTRAEATDVANAVLD 339

[Close]

BLAST of MELO3C027470 vs. TAIR 10
Match: AT3G52990.1 (Pyruvate kinase family protein )

HSP 1 Score: 83.2 bits (204), Expect = 7.0e-17
Identity = 42/49 (85.71%), Postives = 46/49 (93.88%), Query Frame = 0


Query: 5   EVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +VFLFQK ALY+CNMAGKPAV+TRVVDSMT+NLRPTRA AT VANAVLD
Sbjct: 291 KVFLFQKAALYKCNMAGKPAVLTRVVDSMTDNLRPTRAEATDVANAVLD 339

[Close]

BLAST of MELO3C027470 vs. TAIR 10
Match: AT3G52990.2 (Pyruvate kinase family protein )

HSP 1 Score: 83.2 bits (204), Expect = 7.0e-17
Identity = 42/49 (85.71%), Postives = 46/49 (93.88%), Query Frame = 0


Query: 5   EVFLFQKTALYRCNMAGKPAVVTRVVDSMTNNLRPTRAVATVVANAVLD 54
           +VFLFQK ALY+CNMAGKPAV+TRVVDSMT+NLRPTRA AT VANAVLD
Sbjct: 238 KVFLFQKAALYKCNMAGKPAVLTRVVDSMTDNLRPTRAEATDVANAVLD 286

[Close]

BLAST of MELO3C027470 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 56.2 bits (134), Expect = 9.1e-09
Identity = 29/50 (58.00%), Postives = 39/50 (78.00%), Query Frame = 0


Query: 5   EVFLFQKTALYRCNMAGKPAV-VTRVVDSMTNNLRPTRAVATVVANAVLD 54
           ++FL QK  +Y+CN+AGKP V  T++++SM  + RPTRA AT VANAVLD
Sbjct: 275 KIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLD 324

[Close]

BLAST of MELO3C027470 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 56.2 bits (134), Expect = 9.1e-09
Identity = 29/50 (58.00%), Postives = 39/50 (78.00%), Query Frame = 0


Query: 5   EVFLFQKTALYRCNMAGKPAV-VTRVVDSMTNNLRPTRAVATVVANAVLD 54
           ++FL QK  +Y+CN+AGKP V  T++++SM  + RPTRA AT VANAVLD
Sbjct: 275 KIFLAQKLMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLD 324

The following BLAST results are available for this feature:

BLAST of MELO3C027470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B8BJ39	1.3e-15	85.71	Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_35105...	[more]
Q2RAK2	1.3e-15	85.71	Pyruvate kinase 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g01...	[more]
B8BM17	1.3e-15	85.71	Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_37456...	[more]
Q2QXR8	1.3e-15	85.71	Pyruvate kinase 2, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g01...	[more]
Q42806	3.7e-07	56.00	Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1	[more]

BLAST of MELO3C027470 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0036915.1	7.0e-19	100.00	pyruvate kinase 1, cytosolic-like isoform X2 [Cucumis melo var. makuwa]	[more]
KAA0054856.1	6.5e-17	92.45	pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa]	[more]
KAA0054848.1	1.1e-16	92.45	pyruvate kinase 2, cytosolic-like [Cucumis melo var. makuwa]	[more]
KAA0035250.1	1.5e-16	92.45	phagocyte signaling-impaired protein [Cucumis melo var. makuwa]	[more]
KAA0046425.1	5.5e-16	90.57	pyruvate kinase 1, cytosolic-like [Cucumis melo var. makuwa]	[more]

BLAST of MELO3C027470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T5Z6	3.4e-19	100.00	Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold9475G000...	[more]
A0A5A7UKP0	3.2e-17	92.45	Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold406G0064...	[more]
A0A5A7UIR4	5.4e-17	92.45	Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold406G0054...	[more]
A0A5A7SW39	7.0e-17	92.45	Phagocyte signaling-impaired protein OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A5A7TYG6	2.7e-16	90.57	Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold149G0018...	[more]

BLAST of MELO3C027470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G36580.1	7.0e-17	85.71	Pyruvate kinase family protein	[more]
AT3G52990.1	7.0e-17	85.71	Pyruvate kinase family protein	[more]
AT3G52990.2	7.0e-17	85.71	Pyruvate kinase family protein	[more]
AT5G63680.1	9.1e-09	58.00	Pyruvate kinase family protein	[more]
AT5G08570.1	9.1e-09	58.00	Pyruvate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015793	Pyruvate kinase, barrel	PFAM	PF00224	PK	coord: 4..53 e-value: 1.8E-12 score: 46.8
IPR040442	Pyruvate kinase-like domain superfamily	GENE3D	3.20.20.60		coord: 3..53 e-value: 1.2E-14 score: 55.7
IPR001697	Pyruvate kinase	PANTHER	PTHR11817	PYRUVATE KINASE	coord: 5..53
None	No IPR available	PANTHER	PTHR11817:SF98	PYRUVATE KINASE	coord: 5..53
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 5..53

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C027470.1	MELO3C027470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0030955	potassium ion binding
molecular_function	GO:0004743	pyruvate kinase activity