MELO3C027303 (gene) Melon (DHL92) v4

Overview
NameMELO3C027303
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionPectinesterase
Locationchr12: 23536915 .. 23538230 (+)
RNA-Seq ExpressionMELO3C027303
SyntenyMELO3C027303
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAAAGTAAGAACGGTATCATTAGATGGATCGGGCAATTTCAAACGCATTAGTGATGCGATTGTAGCAGCTCCTAACAATTCCAATACGAGATTTTATATTCATGTAGCCCCCGGTACTTACCATGAAGCTATTCAAATTCCAAAGGCTAAGAAGTTCATTGCTTTGATAGGCGATGATGCATTCACGACCATTATCATAGACAACAGAAGTAATGGAACCGGATTCAAGACCAACAACTCTGCAACCCTTAGTAAGCTTCTAATTTCTATATCTTTTTTTAAATTATGGCCATTGATATTCACACCAAAATTTTGAAGATCTTAAAGTTTCAATTTTTTTTTTATAAACTTGAGATGTTCTTTACTTAAAATCAGTGTTTTATTAGTTGTATTTTAAAATAAATATAGAACAAAAATAAGGAGACATCAGTTTTATCTTTTTTTTTTTAGAGTAGTACAGTAACTGAGGTTATGGAATTTCAGCTACAATTATGTTTTAATTTTGAAGTAGGAATAAAGAGTATGACTAAAGGGTTTTCAATCAACAAACTTGGAGAAATGTGTTGGTTTAAAGAATTATCATATACATTATTGAATTTGTTTCCTGATTTTATTTTTATTTGATGCAGCCGTCCAAGGTAACAACTTTATGGCAAAATCTTTAACGTTCGAAAACTCAGCTGGACCTCAAAACGGTCAGGCAGTAGCGGTCTTCGACAAAGCCGACCACACTGCTTACTACAAGTGCAGATTTTTGGGTTTTCAAGACACGTTGTATGTCAATGGAAAGTACCAATTCTTTAAGGAGTCTAACATATATGGAAGCGTTGATTTCATCTTTGGACATGGGCGAGTAATGTTTCAAGATTGCAATATATATGCTCGGATGCCGAGCAATTCTATCACAGTCACAGCACAATCCAAAGATTTTTTACGGTCTATCAGCGGTTTTTCATTTCAAAACTATACGGTCACAGTTTTACCTGAAATATCTTCAAATAAACAAAACGTTAAAGTCTTTTTGGGTCGTCTATGGAGACAGTATTCAAACGTTGTGTTTATGGAATCATTTCTCGATGATGTGGTAGCGTCGGAAGGGTGGATGGAGTGGAAAGGGGTACCCATTAACAACTTGTTCTATGGAGAGTTCAACAACTACGGTCCTGGGGCTGACGTTTCCAAAAGAGTTAATTGGACTGGTTATCATATATTAAATAAGGAATCAGCCTTTCGTTTTACGGTAGATAACTTTATTAATAGATCTAAATGGTTGCATGAAACTGGGATTCCGTTTAGACATGGACTCCTTACCTAA

mRNA sequence

TTAAAGTAAGAACGGTATCATTAGATGGATCGGGCAATTTCAAACGCATTAGTGATGCGATTGTAGCAGCTCCTAACAATTCCAATACGAGATTTTATATTCATGTAGCCCCCGGTACTTACCATGAAGCTATTCAAATTCCAAAGGCTAAGAAGTTCATTGCTTTGATAGGCGATGATGCATTCACGACCATTATCATAGACAACAGAAGTAATGGAACCGGATTCAAGACCAACAACTCTGCAACCCTTACCGTCCAAGGTAACAACTTTATGGCAAAATCTTTAACGTTCGAAAACTCAGCTGGACCTCAAAACGGTCAGGCAGTAGCGGTCTTCGACAAAGCCGACCACACTGCTTACTACAAGTGCAGATTTTTGGGTTTTCAAGACACGTTGTATGTCAATGGAAAGTACCAATTCTTTAAGGAGTCTAACATATATGGAAGCGTTGATTTCATCTTTGGACATGGGCGAGTAATGTTTCAAGATTGCAATATATATGCTCGGATGCCGAGCAATTCTATCACAGTCACAGCACAATCCAAAGATTTTTTACGGTCTATCAGCGGTTTTTCATTTCAAAACTATACGGTCACAGTTTTACCTGAAATATCTTCAAATAAACAAAACGTTAAAGTCTTTTTGGGTCGTCTATGGAGACAGTATTCAAACGTTGTGTTTATGGAATCATTTCTCGATGATGTGGTAGCGTCGGAAGGGTGGATGGAGTGGAAAGGGGTACCCATTAACAACTTGTTCTATGGAGAGTTCAACAACTACGGTCCTGGGGCTGACGTTTCCAAAAGAGTTAATTGGACTGGTTATCATATATTAAATAAGGAATCAGCCTTTCGTTTTACGGTAGATAACTTTATTAATAGATCTAAATGGTTGCATGAAACTGGGATTCCGTTTAGACATGGACTCCTTACCTAA

Coding sequence (CDS)

AAAGTAAGAACGGTATCATTAGATGGATCGGGCAATTTCAAACGCATTAGTGATGCGATTGTAGCAGCTCCTAACAATTCCAATACGAGATTTTATATTCATGTAGCCCCCGGTACTTACCATGAAGCTATTCAAATTCCAAAGGCTAAGAAGTTCATTGCTTTGATAGGCGATGATGCATTCACGACCATTATCATAGACAACAGAAGTAATGGAACCGGATTCAAGACCAACAACTCTGCAACCCTTACCGTCCAAGGTAACAACTTTATGGCAAAATCTTTAACGTTCGAAAACTCAGCTGGACCTCAAAACGGTCAGGCAGTAGCGGTCTTCGACAAAGCCGACCACACTGCTTACTACAAGTGCAGATTTTTGGGTTTTCAAGACACGTTGTATGTCAATGGAAAGTACCAATTCTTTAAGGAGTCTAACATATATGGAAGCGTTGATTTCATCTTTGGACATGGGCGAGTAATGTTTCAAGATTGCAATATATATGCTCGGATGCCGAGCAATTCTATCACAGTCACAGCACAATCCAAAGATTTTTTACGGTCTATCAGCGGTTTTTCATTTCAAAACTATACGGTCACAGTTTTACCTGAAATATCTTCAAATAAACAAAACGTTAAAGTCTTTTTGGGTCGTCTATGGAGACAGTATTCAAACGTTGTGTTTATGGAATCATTTCTCGATGATGTGGTAGCGTCGGAAGGGTGGATGGAGTGGAAAGGGGTACCCATTAACAACTTGTTCTATGGAGAGTTCAACAACTACGGTCCTGGGGCTGACGTTTCCAAAAGAGTTAATTGGACTGGTTATCATATATTAAATAAGGAATCAGCCTTTCGTTTTACGGTAGATAACTTTATTAATAGATCTAAATGGTTGCATGAAACTGGGATTCCGTTTAGACATGGACTCCTTACCTAA

Protein sequence

KVRTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHETGIPFRHGLLT
Homology
BLAST of MELO3C027303 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 2.9e-75
Identity = 140/309 (45.31%), Postives = 204/309 (66.02%), Query Frame = 0

Query: 4   TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT 63
           +V++DG+GNF  I+DA+ AAPN S TRF I++  G Y E +++PK K  I  IGD    T
Sbjct: 223 SVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKT 282

Query: 64  IIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC 123
           +I  NRS   G+ T  + T+ V+G  ++AK ++F NSAGP   QAVA    +DH+A+Y+C
Sbjct: 283 VIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRC 342

Query: 124 RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPS--NSITVTAQS 183
            F G+QDTLYV+   QF++E +IYG++DFIFG+  V+FQ+ ++YAR P+  + I  TAQS
Sbjct: 343 EFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQS 402

Query: 184 KDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGW 243
           ++     +G S  N  +   P++   K+N K +LGR WR+YS  V ++SF+DD++   GW
Sbjct: 403 RNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGW 462

Query: 244 MEW-KGVPINNLFYGEFNNYGPGADVSKRVNWTGY-HILNKESAFRFTVDNFINRSKWLH 303
           +E  K   +  L+YGE+ N GPGA+++KRV W G+  I N+  A +FTV  FI+ S WL+
Sbjct: 463 LEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLN 522

Query: 304 ETGIPFRHG 309
            TGIPF  G
Sbjct: 523 STGIPFSLG 531

BLAST of MELO3C027303 vs. ExPASy Swiss-Prot
Match: O48711 (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PME12 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.5e-74
Identity = 137/308 (44.48%), Postives = 191/308 (62.01%), Query Frame = 0

Query: 5   VSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTTI 64
           V+ DG+GNF  I++AI  APN SN R  I+V  G Y E I IP  K  I LIGD +  T 
Sbjct: 240 VAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVTF 299

Query: 65  IIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKCR 124
           I  NRS G G+ T  SATL V G  F+A+ +   N+AGP+  QAVA+   AD  A Y+C 
Sbjct: 300 ITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRCV 359

Query: 125 FLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYAR--MPSNSITVTAQSK 184
             G+QDTLY +   QF++E +IYG++D+IFG+  V+FQ CNI ++  MP     +TAQS+
Sbjct: 360 IDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQSR 419

Query: 185 DFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWM 244
           D     +G S QN ++    ++ ++   VK +LGR WR++S  V MES++D+ +   GW 
Sbjct: 420 DTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGSGWS 479

Query: 245 EWK-GVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHET 304
           +W  G  ++ L+YGE+NN GPG++  KRVNW G+HI+  E AF FT   FI    WL  T
Sbjct: 480 KWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYEDAFNFTATEFITGDGWLGST 539

Query: 305 GIPFRHGL 310
             P+ +G+
Sbjct: 540 SFPYDNGI 547

BLAST of MELO3C027303 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.5e-74
Identity = 134/314 (42.68%), Postives = 197/314 (62.74%), Query Frame = 0

Query: 4   TVSLDGSGNFKRISDAIVAAPNNSNTR---FYIHVAPGTYHEAIQIPKAKKFIALIGDDA 63
           TVS DG+GNF  I+ A+ AAPNN++     F I+V  G Y E I I K K+++ +IGD  
Sbjct: 266 TVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGI 325

Query: 64  FTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAY 123
             T++  NRS   G+ T NSAT  V   NF+A ++TF N+AGP+  QAVA+   AD + +
Sbjct: 326 NQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIF 385

Query: 124 YKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYAR--MPSNSITVT 183
           Y C F  +QDTLY +   QF++E ++YG+VDFIFG+  V+FQ+CN+Y R  MP+    +T
Sbjct: 386 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAIT 445

Query: 184 AQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVAS 243
           AQ +      +G S QN T+    ++ S+   VK +LGR W++YS  V+M+S++D  V  
Sbjct: 446 AQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEP 505

Query: 244 EGWMEWKG-VPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKW 303
            GW EW G   ++ L+Y E+NN GPG++ + RV W GYH++N   A  FTV      + W
Sbjct: 506 VGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADW 565

Query: 304 LHETGIPFRHGLLT 312
           + +TG+P+  GL++
Sbjct: 566 IWKTGVPYTSGLIS 579

BLAST of MELO3C027303 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 272.7 bits (696), Expect = 5.1e-72
Identity = 131/314 (41.72%), Postives = 198/314 (63.06%), Query Frame = 0

Query: 4   TVSLDGSGNFKRISDAIVAAPNNSNTR---FYIHVAPGTYHEAIQIPKAKKFIALIGDDA 63
           TV+ +G+GNF  I++A+ +APN ++     F I+V  G Y E + I K K+++ +IGD  
Sbjct: 260 TVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDGI 319

Query: 64  FTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAY 123
             T++  NR+   G+ T NSAT  V   NF+A ++TF N+AGP+  QAVA+   AD + +
Sbjct: 320 NRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSIF 379

Query: 124 YKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNS--ITVT 183
           Y C F  +QDTLY +   QF++E +IYG+VDFIFG+  V+FQDCN+Y R P  +    +T
Sbjct: 380 YSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAIT 439

Query: 184 AQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVAS 243
           AQ +      +G S  N T+    ++ S+   VK +LGR W++YS  VFM+S++D+VV  
Sbjct: 440 AQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEP 499

Query: 244 EGWMEWKG-VPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKW 303
            GW EW G   ++ L+Y E+NN G G+  + RV W GYH++N   A  FTV+NF+    W
Sbjct: 500 VGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDGW 559

Query: 304 LHETGIPFRHGLLT 312
           + ++G+P+  GLL+
Sbjct: 560 MVQSGVPYISGLLS 573

BLAST of MELO3C027303 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.1e-71
Identity = 136/308 (44.16%), Postives = 195/308 (63.31%), Query Frame = 0

Query: 5   VSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTTI 64
           V+ DGSGN K I +A+ AA     +R+ I++  GTY+E I++    K I  +GD    TI
Sbjct: 205 VAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEV--KLKNIMFVGDGIGKTI 264

Query: 65  IIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKCR 124
           I  ++S G G  T  SAT+ V G+NF+A+ +T  N+AGP N QAVA+   +D + +Y+C 
Sbjct: 265 ITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCS 324

Query: 125 FLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSN-SITVTAQSKD 184
           F G+QDTLYV+ + QF++E +IYG+VDFIFG+  V+ Q+CNI+AR P N + T+TAQ + 
Sbjct: 325 FEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQGRT 384

Query: 185 FLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWME 244
                +G    N  VT   ++   + +VK FLGR W+QYS  V++++FLD ++   GWME
Sbjct: 385 DPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWME 444

Query: 245 WKG-VPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKES-AFRFTVDNFINRSKWLHET 304
           W G   +N L+Y E+ N GPG+  + RV W GYH+L   S   +FTV NFI  + WL  T
Sbjct: 445 WSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPAT 504

Query: 305 GIPFRHGL 310
            +PF  GL
Sbjct: 505 NVPFTSGL 510

BLAST of MELO3C027303 vs. NCBI nr
Match: TYJ99208.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 635.2 bits (1637), Expect = 3.0e-178
Identity = 307/308 (99.68%), Postives = 308/308 (100.00%), Query Frame = 0

Query: 4   TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT 63
           TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT
Sbjct: 12  TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT 71

Query: 64  IIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC 123
           IIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC
Sbjct: 72  IIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC 131

Query: 124 RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKD 183
           RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHG+VMFQDCNIYARMPSNSITVTAQSKD
Sbjct: 132 RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGQVMFQDCNIYARMPSNSITVTAQSKD 191

Query: 184 FLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWME 243
           FLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWME
Sbjct: 192 FLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWME 251

Query: 244 WKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHETGI 303
           WKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHETGI
Sbjct: 252 WKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHETGI 311

Query: 304 PFRHGLLT 312
           PFRHGLLT
Sbjct: 312 PFRHGLLT 319

BLAST of MELO3C027303 vs. NCBI nr
Match: KAA0055286.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 602.1 bits (1551), Expect = 2.8e-168
Identity = 292/308 (94.81%), Postives = 300/308 (97.40%), Query Frame = 0

Query: 4   TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT 63
           TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT
Sbjct: 12  TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT 71

Query: 64  IIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC 123
           IIIDNRSNGTGFKTNNSATLTVQG+NFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC
Sbjct: 72  IIIDNRSNGTGFKTNNSATLTVQGDNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC 131

Query: 124 RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKD 183
           RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKD
Sbjct: 132 RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKD 191

Query: 184 FLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWME 243
           FLRSISGFSFQN TVTV PEISSNKQNVKVFLGR WRQYSNVVFMESFLDDVVASEGWME
Sbjct: 192 FLRSISGFSFQNCTVTVSPEISSNKQNVKVFLGRPWRQYSNVVFMESFLDDVVASEGWME 251

Query: 244 WKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHETGI 303
           WKGVP+NNLFYGEFNN+GPGADVSKRVNWTGYH+L+K+SA  FTVDNFI+ S+WL ETGI
Sbjct: 252 WKGVPVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKSAIGFTVDNFIDGSEWLAETGI 311

Query: 304 PFRHGLLT 312
           PFR GLLT
Sbjct: 312 PFRRGLLT 319

BLAST of MELO3C027303 vs. NCBI nr
Match: XP_008465596.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 47, partial [Cucumis melo])

HSP 1 Score: 572.8 bits (1475), Expect = 1.8e-159
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 37  PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 96
           PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT
Sbjct: 1   PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 60

Query: 97  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 156
           FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH
Sbjct: 61  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 120

Query: 157 GRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLG 216
           GRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLG
Sbjct: 121 GRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLG 180

Query: 217 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYH 276
           RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYH
Sbjct: 181 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYH 240

Query: 277 ILNKESAFRFTVDNFINRSKWLHETGIPFRHGLLT 312
           ILNKESAFRFTVDNFINRSKWLHETGIPFRHGLLT
Sbjct: 241 ILNKESAFRFTVDNFINRSKWLHETGIPFRHGLLT 275

BLAST of MELO3C027303 vs. NCBI nr
Match: KAA0055285.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 562.4 bits (1448), Expect = 2.5e-156
Identity = 271/275 (98.55%), Postives = 272/275 (98.91%), Query Frame = 0

Query: 37  PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 96
           PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT
Sbjct: 7   PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 66

Query: 97  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 156
           FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH
Sbjct: 67  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 126

Query: 157 GRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLG 216
           G+VMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVL EISSNKQNVKVFLG
Sbjct: 127 GQVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLLEISSNKQNVKVFLG 186

Query: 217 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYH 276
           RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPI NLFYGEFNNYGPGADVSKRVNWTGYH
Sbjct: 187 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPIKNLFYGEFNNYGPGADVSKRVNWTGYH 246

Query: 277 ILNKESAFRFTVDNFINRSKWLHETGIPFRHGLLT 312
           ILNKESAFRFTVDNFIN SKWLHETGIPFRHGLLT
Sbjct: 247 ILNKESAFRFTVDNFINGSKWLHETGIPFRHGLLT 281

BLAST of MELO3C027303 vs. NCBI nr
Match: XP_004152582.2 (pectinesterase 2 [Cucumis sativus] >KGN64355.1 hypothetical protein Csa_013871 [Cucumis sativus])

HSP 1 Score: 549.7 bits (1415), Expect = 1.7e-152
Identity = 266/306 (86.93%), Postives = 283/306 (92.48%), Query Frame = 0

Query: 4   TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT 63
           TVSLDGSGNFKRISDAI AAPNNSNTRFYIHVAPGTYHE +QIP+ KKFIAL+GDD FTT
Sbjct: 29  TVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKFIALVGDDPFTT 88

Query: 64  IIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC 123
           IIIDNRSNGTGFKTNNSATLTVQG+NFMA+SLTFENSAGPQN QAVAVFDKA+HTAYYKC
Sbjct: 89  IIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTAYYKC 148

Query: 124 RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKD 183
           RFL FQDTLYVNGK QFFKES+IYGSVDFI G+G+VMFQDCNIYARMP NSITVTAQSK 
Sbjct: 149 RFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPINSITVTAQSKY 208

Query: 184 FLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWME 243
            LRS+SGFSFQN TVTV  EISSNKQNVKVFLGR W+QYS VVFMESFLDDVVASEGW+E
Sbjct: 209 ILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVASEGWVE 268

Query: 244 WKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHETGI 303
           W GVP+NNLFYGEFNN GPGADVSKRVNWT YH+L+KESA RFTVDNF+N S+WL ETGI
Sbjct: 269 WIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFTVDNFVNGSEWLPETGI 328

Query: 304 PFRHGL 310
           PFR GL
Sbjct: 329 PFRRGL 334

BLAST of MELO3C027303 vs. ExPASy TrEMBL
Match: A0A5D3BM28 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004180 PE=4 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 1.4e-178
Identity = 307/308 (99.68%), Postives = 308/308 (100.00%), Query Frame = 0

Query: 4   TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT 63
           TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT
Sbjct: 12  TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT 71

Query: 64  IIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC 123
           IIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC
Sbjct: 72  IIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC 131

Query: 124 RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKD 183
           RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHG+VMFQDCNIYARMPSNSITVTAQSKD
Sbjct: 132 RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGQVMFQDCNIYARMPSNSITVTAQSKD 191

Query: 184 FLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWME 243
           FLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWME
Sbjct: 192 FLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWME 251

Query: 244 WKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHETGI 303
           WKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHETGI
Sbjct: 252 WKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHETGI 311

Query: 304 PFRHGLLT 312
           PFRHGLLT
Sbjct: 312 PFRHGLLT 319

BLAST of MELO3C027303 vs. ExPASy TrEMBL
Match: A0A5A7ULX7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290 PE=4 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.4e-168
Identity = 292/308 (94.81%), Postives = 300/308 (97.40%), Query Frame = 0

Query: 4   TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT 63
           TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT
Sbjct: 12  TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT 71

Query: 64  IIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC 123
           IIIDNRSNGTGFKTNNSATLTVQG+NFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC
Sbjct: 72  IIIDNRSNGTGFKTNNSATLTVQGDNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC 131

Query: 124 RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKD 183
           RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKD
Sbjct: 132 RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKD 191

Query: 184 FLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWME 243
           FLRSISGFSFQN TVTV PEISSNKQNVKVFLGR WRQYSNVVFMESFLDDVVASEGWME
Sbjct: 192 FLRSISGFSFQNCTVTVSPEISSNKQNVKVFLGRPWRQYSNVVFMESFLDDVVASEGWME 251

Query: 244 WKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHETGI 303
           WKGVP+NNLFYGEFNN+GPGADVSKRVNWTGYH+L+K+SA  FTVDNFI+ S+WL ETGI
Sbjct: 252 WKGVPVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKSAIGFTVDNFIDGSEWLAETGI 311

Query: 304 PFRHGLLT 312
           PFR GLLT
Sbjct: 312 PFRRGLLT 319

BLAST of MELO3C027303 vs. ExPASy TrEMBL
Match: A0A1S3CP85 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503231 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 8.8e-160
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 37  PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 96
           PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT
Sbjct: 1   PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 60

Query: 97  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 156
           FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH
Sbjct: 61  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 120

Query: 157 GRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLG 216
           GRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLG
Sbjct: 121 GRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLG 180

Query: 217 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYH 276
           RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYH
Sbjct: 181 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYH 240

Query: 277 ILNKESAFRFTVDNFINRSKWLHETGIPFRHGLLT 312
           ILNKESAFRFTVDNFINRSKWLHETGIPFRHGLLT
Sbjct: 241 ILNKESAFRFTVDNFINRSKWLHETGIPFRHGLLT 275

BLAST of MELO3C027303 vs. ExPASy TrEMBL
Match: A0A5A7UNS3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001280 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.2e-156
Identity = 271/275 (98.55%), Postives = 272/275 (98.91%), Query Frame = 0

Query: 37  PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 96
           PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT
Sbjct: 7   PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 66

Query: 97  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 156
           FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH
Sbjct: 67  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 126

Query: 157 GRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLG 216
           G+VMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVL EISSNKQNVKVFLG
Sbjct: 127 GQVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLLEISSNKQNVKVFLG 186

Query: 217 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYH 276
           RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPI NLFYGEFNNYGPGADVSKRVNWTGYH
Sbjct: 187 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPIKNLFYGEFNNYGPGADVSKRVNWTGYH 246

Query: 277 ILNKESAFRFTVDNFINRSKWLHETGIPFRHGLLT 312
           ILNKESAFRFTVDNFIN SKWLHETGIPFRHGLLT
Sbjct: 247 ILNKESAFRFTVDNFINGSKWLHETGIPFRHGLLT 281

BLAST of MELO3C027303 vs. ExPASy TrEMBL
Match: A0A0A0LR14 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.0e-153
Identity = 266/306 (86.93%), Postives = 283/306 (92.48%), Query Frame = 0

Query: 4   TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT 63
           TVSLDGSGNFKRISDAI AAPNNSNTRFYIHVAPGTYHE +QIP+ KKFIAL+GDD FTT
Sbjct: 29  TVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKFIALVGDDPFTT 88

Query: 64  IIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC 123
           IIIDNRSNGTGFKTNNSATLTVQG+NFMA+SLTFENSAGPQN QAVAVFDKA+HTAYYKC
Sbjct: 89  IIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTAYYKC 148

Query: 124 RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNSITVTAQSKD 183
           RFL FQDTLYVNGK QFFKES+IYGSVDFI G+G+VMFQDCNIYARMP NSITVTAQSK 
Sbjct: 149 RFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPINSITVTAQSKY 208

Query: 184 FLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWME 243
            LRS+SGFSFQN TVTV  EISSNKQNVKVFLGR W+QYS VVFMESFLDDVVASEGW+E
Sbjct: 209 ILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVASEGWVE 268

Query: 244 WKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHETGI 303
           W GVP+NNLFYGEFNN GPGADVSKRVNWT YH+L+KESA RFTVDNF+N S+WL ETGI
Sbjct: 269 WIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFTVDNFVNGSEWLPETGI 328

Query: 304 PFRHGL 310
           PFR GL
Sbjct: 329 PFRRGL 334

BLAST of MELO3C027303 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 283.5 bits (724), Expect = 2.1e-76
Identity = 140/309 (45.31%), Postives = 204/309 (66.02%), Query Frame = 0

Query: 4   TVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTT 63
           +V++DG+GNF  I+DA+ AAPN S TRF I++  G Y E +++PK K  I  IGD    T
Sbjct: 223 SVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKT 282

Query: 64  IIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKC 123
           +I  NRS   G+ T  + T+ V+G  ++AK ++F NSAGP   QAVA    +DH+A+Y+C
Sbjct: 283 VIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRC 342

Query: 124 RFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPS--NSITVTAQS 183
            F G+QDTLYV+   QF++E +IYG++DFIFG+  V+FQ+ ++YAR P+  + I  TAQS
Sbjct: 343 EFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQS 402

Query: 184 KDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGW 243
           ++     +G S  N  +   P++   K+N K +LGR WR+YS  V ++SF+DD++   GW
Sbjct: 403 RNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGW 462

Query: 244 MEW-KGVPINNLFYGEFNNYGPGADVSKRVNWTGY-HILNKESAFRFTVDNFINRSKWLH 303
           +E  K   +  L+YGE+ N GPGA+++KRV W G+  I N+  A +FTV  FI+ S WL+
Sbjct: 463 LEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLN 522

Query: 304 ETGIPFRHG 309
            TGIPF  G
Sbjct: 523 STGIPFSLG 531

BLAST of MELO3C027303 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 279.3 bits (713), Expect = 3.9e-75
Identity = 134/314 (42.68%), Postives = 197/314 (62.74%), Query Frame = 0

Query: 4   TVSLDGSGNFKRISDAIVAAPNNSNTR---FYIHVAPGTYHEAIQIPKAKKFIALIGDDA 63
           TVS DG+GNF  I+ A+ AAPNN++     F I+V  G Y E I I K K+++ +IGD  
Sbjct: 266 TVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGI 325

Query: 64  FTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAY 123
             T++  NRS   G+ T NSAT  V   NF+A ++TF N+AGP+  QAVA+   AD + +
Sbjct: 326 NQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIF 385

Query: 124 YKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYAR--MPSNSITVT 183
           Y C F  +QDTLY +   QF++E ++YG+VDFIFG+  V+FQ+CN+Y R  MP+    +T
Sbjct: 386 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAIT 445

Query: 184 AQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVAS 243
           AQ +      +G S QN T+    ++ S+   VK +LGR W++YS  V+M+S++D  V  
Sbjct: 446 AQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEP 505

Query: 244 EGWMEWKG-VPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKW 303
            GW EW G   ++ L+Y E+NN GPG++ + RV W GYH++N   A  FTV      + W
Sbjct: 506 VGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADW 565

Query: 304 LHETGIPFRHGLLT 312
           + +TG+P+  GL++
Sbjct: 566 IWKTGVPYTSGLIS 579

BLAST of MELO3C027303 vs. TAIR 10
Match: AT2G26440.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 279.3 bits (713), Expect = 3.9e-75
Identity = 137/308 (44.48%), Postives = 191/308 (62.01%), Query Frame = 0

Query: 5   VSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTTI 64
           V+ DG+GNF  I++AI  APN SN R  I+V  G Y E I IP  K  I LIGD +  T 
Sbjct: 240 VAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVTF 299

Query: 65  IIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKCR 124
           I  NRS G G+ T  SATL V G  F+A+ +   N+AGP+  QAVA+   AD  A Y+C 
Sbjct: 300 ITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRCV 359

Query: 125 FLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYAR--MPSNSITVTAQSK 184
             G+QDTLY +   QF++E +IYG++D+IFG+  V+FQ CNI ++  MP     +TAQS+
Sbjct: 360 IDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQSR 419

Query: 185 DFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWM 244
           D     +G S QN ++    ++ ++   VK +LGR WR++S  V MES++D+ +   GW 
Sbjct: 420 DTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGSGWS 479

Query: 245 EWK-GVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHET 304
           +W  G  ++ L+YGE+NN GPG++  KRVNW G+HI+  E AF FT   FI    WL  T
Sbjct: 480 KWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYEDAFNFTATEFITGDGWLGST 539

Query: 305 GIPFRHGL 310
             P+ +G+
Sbjct: 540 SFPYDNGI 547

BLAST of MELO3C027303 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 272.7 bits (696), Expect = 3.6e-73
Identity = 131/314 (41.72%), Postives = 198/314 (63.06%), Query Frame = 0

Query: 4   TVSLDGSGNFKRISDAIVAAPNNSNTR---FYIHVAPGTYHEAIQIPKAKKFIALIGDDA 63
           TV+ +G+GNF  I++A+ +APN ++     F I+V  G Y E + I K K+++ +IGD  
Sbjct: 260 TVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDGI 319

Query: 64  FTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAY 123
             T++  NR+   G+ T NSAT  V   NF+A ++TF N+AGP+  QAVA+   AD + +
Sbjct: 320 NRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSIF 379

Query: 124 YKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPSNS--ITVT 183
           Y C F  +QDTLY +   QF++E +IYG+VDFIFG+  V+FQDCN+Y R P  +    +T
Sbjct: 380 YSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAIT 439

Query: 184 AQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVAS 243
           AQ +      +G S  N T+    ++ S+   VK +LGR W++YS  VFM+S++D+VV  
Sbjct: 440 AQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEP 499

Query: 244 EGWMEWKG-VPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKW 303
            GW EW G   ++ L+Y E+NN G G+  + RV W GYH++N   A  FTV+NF+    W
Sbjct: 500 VGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDGW 559

Query: 304 LHETGIPFRHGLLT 312
           + ++G+P+  GLL+
Sbjct: 560 MVQSGVPYISGLLS 573

BLAST of MELO3C027303 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 270.8 bits (691), Expect = 1.4e-72
Identity = 133/308 (43.18%), Postives = 193/308 (62.66%), Query Frame = 0

Query: 10  SGNFKRISDAIVAAPNNSNTR---FYIHVAPGTYHEAIQIPKAKKFIALIGDDAFTTIII 69
           S NF  I+DAI AAPNN+      F I+   G Y E I +P  KK + L+GD    TII 
Sbjct: 310 SDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIIT 369

Query: 70  DNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVFDKADHTAYYKCRFL 129
            N +   G+ T N ++  V G  FMA  +TF N+AGP+  QAVA+ + A+ +++Y+C F 
Sbjct: 370 GNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFE 429

Query: 130 GFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMP--SNSITVTAQSKDF 189
           G+QDTLYV+   QF++E +IYG+VDFIFG+   +FQ+CNIYAR P       +TA  +  
Sbjct: 430 GYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLD 489

Query: 190 LRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFLDDVVASEGWMEW 249
               +G S  N T+   P++++  ++   FLGR W+ YS  VFM+S++ D+V   GW+EW
Sbjct: 490 PNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEW 549

Query: 250 KG-VPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFINRSKWLHETGI 309
            G + ++ ++YGE++N+GPGA+ ++RV W GY++LN   A  FTV NF     WL +T I
Sbjct: 550 NGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDI 609

Query: 310 PFRHGLLT 312
           PF  GLL+
Sbjct: 610 PFYGGLLS 617

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O814152.9e-7545.31Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
O487115.5e-7444.48Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3... [more]
Q9SRX45.5e-7442.68Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
Q8RXK75.1e-7241.72Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
O048871.1e-7144.16Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
TYJ99208.13.0e-17899.68putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa][more]
KAA0055286.12.8e-16894.81putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa][more]
XP_008465596.11.8e-159100.00PREDICTED: probable pectinesterase/pectinesterase inhibitor 47, partial [Cucumis... [more]
KAA0055285.12.5e-15698.55putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa][more]
XP_004152582.21.7e-15286.93pectinesterase 2 [Cucumis sativus] >KGN64355.1 hypothetical protein Csa_013871 [... [more]
Match NameE-valueIdentityDescription
A0A5D3BM281.4e-17899.68Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00418... [more]
A0A5A7ULX71.4e-16894.81Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290... [more]
A0A1S3CP858.8e-160100.00Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503231 PE=4 SV=1[more]
A0A5A7UNS31.2e-15698.55Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001280... [more]
A0A0A0LR148.0e-15386.93Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G02300.12.1e-7645.31Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G02810.13.9e-7542.68Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G26440.13.9e-7544.48Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.13.6e-7341.72Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10720.21.4e-7243.18Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 1..309
e-value: 1.9E-107
score: 361.3
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 4..293
e-value: 3.5E-100
score: 334.6
NoneNo IPR availablePANTHERPTHR31707:SF149PECTINESTERASE/PECTINESTERASE INHIBITOR 17-RELATEDcoord: 4..307
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 4..307
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 146..155
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 3..307

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C027303.1MELO3C027303.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
molecular_function GO:0030599 pectinesterase activity