MELO3C026934.jh1 (gene) Melon (Harukei-3) v1.41

Overview


Name	MELO3C026934.jh1
Type	gene
Organism	Cucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Description	dihydrofolate synthetase isoform X1
Location	chr06: 8848033 .. 8857361 (-)
RNA-Seq Expression	MELO3C026934.jh1
Synteny	MELO3C026934.jh1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSstart_codonstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGTCTTCAAATATCATCACCACTGTCGCCCACAGATTCTCGGAAGCCTTCTCCTAAATCATTTCATTGGTGAATGCCCTTCTATCAGTTCCCGAAATGGGTCCAAACAATGCTTCGCCACTCACTCAGAAGATCAACACATGACTGAATTCATGGAATATTTAGATTCCCTGAAGAACTACGAGAAATTGGGTGTGCCCAGAGGTGCAGGCACTGATTCTGATGATGGGTTTGACCTGGGAAGGATGAGAAGGCTGATGGAGCGACTGGGTAATCCACAATCCAAGTTTAAGGTTGCCTTGCCTCTTAGTTTTCTTAGTCTTTACTAAATCTTGCAGTTTTGGACTGACACAATTCATCTGTAGCTCAACTGAGTTAAATTAGACTTAAAAAGCCTAAAAAAAGTCGGTAGATTTTTAATCTTTTATTCATACTAATACTCATCTCATGGGAAATGATATTACCTATGTTTGAAAATTTCATATGAGAAATGTAAGGTTATATTGACTAATGGTAGGACAATTTTAGGCAATTCACATTGCTGGAACGAAGGGAAAAGGGTCGATTGCGGCATTCCTTTCGAACATTTTACGAGCAGAGGGATACTCAGTTGGTTGTTATACTAGGTACAGCCCTTTTGCAATAATTATCAAATTTTCTGTATAGTCTTACTCGGGATTGATTTGAGAAGGTTTAACTTCGTGATTGTGAATTTCTTCATGCGTAGTCCTCATATTGAAACTTTAAGGGAACGTATTTCACTTGGAAGATCTGGAGAGATGGTCTCTGGGAAGGCACTAAATTCTCTTTTCAAAAGGAATAAAGAGGTTTTTGATCAGTCAGTAGAGCTTGAACATGGACGTATAAGTCACTTTGAGGTATTAATTCGGGCTTCTGTTGTTGTGATTGAAAATTCTCTAGGAACTCAAATGGCTTATTTGCTGCTTCTGATTCTTCTACATGATTTTATTAAGCAAAATGATGTTCCATGTCTAAGTTTTCACTTTGTAAATTTGTGTGTCATGTGATCTTTAATAATATATCCTGCATCTTTTTCTTTTTTCTTTTGGTTTCATCTAGAAAGATATAAATTTTTCATGTCAATGATAAACCTTTGAACTCTTAAAAGTTCATTGGACCAATTCAGTCATTGATGTTTTCTTTAATCTTTTAGACGAGCTGATGAAATTCTCCCAGGTCCTTACTGCTATGGCATTCTCGCTCTTTGCTCAAGAGAATGTTGATGTTGCAGTCATTGAGGTTCACTTTCTTTTAACTAATTTTTTTCCTTGACAAGTATTTTTGTTTGTTTCTCCAGCAAAAACTTGTAAGCCATTGACAGTATGTTCTTATTTTAACGGAAACAGGCTGGACTTGGTGGTGCACGGGATGCGACAAACATAATTTGCAGCTCTGGACTTGCTGCTGCAGTCATAACTTCGATTGGAGAGGAACACATGGCTGCCCTGGGTGGCTCTTTGGAAAGCATTGCAATGGCAAAGGCTGGAATAATTAAACGTGGTTGTCCAGTTAGGTTGTTTTCATGATGTAATTTTGATGCTGTTTGACTTTGTCAAGCTTTTGATATTCATTATCCACGCAATAGTTAGTTTTTCTTTTCTAATGAGTTGAAATAACCTACAAGAAGTGAAGAGTTGACTAAAGTCAGCCCGAACTTGCATAAGATCAGAGAAATGTTTTCTGAATGGCTGTAATTATTGTTTGAAATCATAATTGTGGTTGAAAGACTGAACTGAAGTTTTTGTCATAATGAACTTCAAGCACCACACAAGAAAACTAAATTGAAATCTGTTTGTCAAATTATATTGTCATATGGGAAGGTATCAAATTGGTCTATTGCAGTAAATGACGTAGAAGAAAATGTCCCCTAACGTGATTGAATTTTCTCTCCTAAAGTTGTTGATGTTAGTCTATGTGTTTGTTTGTCTGTGTCTATGTGTGAGAGAAGTTTATTCTTCAACCACTATTGTGATAATATTCTCCTGATTCATTCTCTTTTTCGTTCTGAAATTTATTCCTAAAAGGCTCCTGAATTTAATGTCAGACAATTCTGGGTGGTTCATTCCTTCCAAGAATTGAGTACATTCTTCGCGATAAAGCATTGTCCATGTCTTCACCTGTAATAGCAGTCTCAGATCCTGGAAACAGAAGTACCATAAAAGGTGTAAACATGCTCAATGGAGGACTTTGCCAATGCTGCGACATAGTAATTCAAATAGACAATGAAGTATGGCTAGCTCATGTATTCTTAAACAAATATAATTTCACTCCTTGAACTTCAAGCAAGAGTTCTTTAATCTCTTTTTCAATGCAGTTCGTCGAGTTATTGGATGTCAACCTCCGCATGCTTGGACGTCATCAACTTCAGAATGCAGCCACTGCAACTTGTGTTATTCTTACTCTCCGCAATCTAGGTATAAATATCATTTTGTATATTTGATTTGATATCCGTACTTTCATTTAGAGAATAAATATGTGATGCGTAATGTATTTCCATGACACGTTTACTTAAGGTGCATCTACACTTTCTTTTTGAAGTTTCTGAACAGTTTGTGCCCTTTGCATGTGAGACCTGTGCAGACAGGATGCTTTAGAGACAAATGTACTAAAGTCTTAAAAAAAGATGATAATGATGCAAGATAGATTGTAGTTTATTTCACTGAATACTTTTAATATCTCCCTAATGAGAAATAATTCAGGTTGGAGAATTTCGGATGCATCTATCAGGAGTGGACTAGAGCAAACATTCCTTATTGGTAGGAGTCATTTCTTAGCAGCCAAGGAAGCTGAGGTTCTTGGACTACCTGGAGCAACAATATTGCTGGATGGAGGTGCATTCATATTTCCATTGTGCTCATGACAATTTGGTCTGTTGGAACCTGATCATTGTGCTCATGCTGCGTCTTCTCTATCGTATTGTACTTAAAGATTTTTGGCAGTTTGATGTTCAGTTCATGGGCTCTCTTTTCACTGTTATTGGTAAATGTTTATAGTTGGTCTGCTGATGCCTGACGCTTGAAGGTTTGCAGAATATTGTAGATCAACTTAGTGTTTTCGACCTGCATCAACTGATATATATATATATTTGAGTTATGTTGTTCTGGATATTGGTGCTATGTCTTGCATTCTTTTAGACTGCATTTCTAATTGGTTCTTTAATTTTTTCACAGCACATACCAAAGACTCTGCTAAAGCATTAGTGGATACCATTCAAATGGCTTTCCCTGAGGCTCGATTGGCTCTTGTGATTGCAATGGCTAGTGATAAGAATCACGTTGATTTTGCCAGAGAATTCCTTCAAGGTTAGCCTTCTCCTCTGCTAAGAGTTGGTCTCATATTTTCTTCAGCACAGAAGTTTCTTCTTGACTGTAAATTCTTCGATATTCTTGGAACTTTTGGATTATATAGGCCACATCTGGTTCTTATAACCATGTTTCTTTTGAATCATATGAGAATGTCTGCAGAGATAGATTAGCTTAAGTGTGCACAGATTGAAATAGATGACTGGAACAAAGTGTGTTTAACAGAAGTTGGTAAGAGAAAATATGTTTAAAGAGTGAATAAACTAAAACTGGTAGCGACATTTTTGTCTAGTGAATTTATGATAGGTTCTAGAAATTTTCATTTTAGTTCTTAAATATTAGTCGAGGTTTCAAATTTTAGTTAAAAGAAGCTTAACAAACGAAAACAGAGAAAACTTTATCTTCCTTATTTATCAAATAGGAGACTGAAAATGAGGTGTGGACTCCATCAAATCACATGCTTCCTCTTCTACCTTTGCTCTCCATTTTCTAAATACAAAATGAAACCTTTAGTTGAAGTAATGAAAAGTAACGAATAAACTTTCCCAAGGGAGAAATAAAAAAGCAAAGAGAAAATATACATAAAGTGAGCTTACTGAGCACCCAAATTAATACAAATATCCTGAGAAGCAAAATCAATCAATAATTTAGGAAAATGATATCATTGATAAGCTTTTAATTTGGAAGTATTGAAGCCCTCCAGGCAACCCTGCTGCTTATTCTCTAAAGTCTTTTGATTATGGTCCATCAAATCTTAGAAATGATTGCAATTTAAACAAAGCAATAACTTTTTCGAGAGATTTTGTTTAATTTTTTATATGTTATGCGATGTCATGTAATTTTGTTCGTATCTGTCTACTTTGATGATGTGACAGTGAGATGTAGCAATAAAATGCTGACAGTCGGCAGTTAATTGACTTTCAGGTGGAAAATTAGAGTGCGTTGTCTTAACTGAAGCCCACATTGGTGGAGGCAAATCAAGGACAACGTCAGCTGCTTTTCTAAGAGACTGCTGGATCCAAGCATCCATTGAATTGGGGATCCCTATTTCTTTAGAAACTAAAGATGCAGAAGTTTCCTTCACAAGCAAACTCGAAAACAGACCGGTATTGACTACTGAAACCTCGTTATTACACGCCATAAAAATTGCTGCTGAGATTCTTAAGCAAAGAACGAAAGGACAGCAAAGCCTTGTCGTAGTATCTGGCTCTCTGCATGCTGTTTCAATGGTGTTATCTTCTCTTCATTCTTGAAGTCTTTATTTGTAAAAGCACTTCTTTTGTATGTACTTTGAGCATAGTTCTTGAATATTGCAGATTATTTTCTTAGCATATTTCTATGAATTATCTGAAGTGTGGCATTAGTGGCAGGGAAAAGTAATGAAACAAACCTTACAAAGGGTAAGTTGTTATTGCTC

mRNA sequence

ATGAATGTCTTCAAATATCATCACCACTGTCGCCCACAGATTCTCGGAAGCCTTCTCCTAAATCATTTCATTGGTGAATGCCCTTCTATCAGTTCCCGAAATGGGTCCAAACAATGCTTCGCCACTCACTCAGAAGATCAACACATGACTGAATTCATGGAATATTTAGATTCCCTGAAGAACTACGAGAAATTGGGTGTGCCCAGAGGTGCAGGCACTGATTCTGATGATGGGTTTGACCTGGGAAGGATGAGAAGGCTGATGGAGCGACTGGGTAATCCACAATCCAAGTTTAAGGCAATTCACATTGCTGGAACGAAGGGAAAAGGGTCGATTGCGGCATTCCTTTCGAACATTTTACGAGCAGAGGGATACTCAGTTGGTTGTTATACTAGTCCTCATATTGAAACTTTAAGGGAACGTATTTCACTTGGAAGATCTGGAGAGATGGTCTCTGGGAAGGCACTAAATTCTCTTTTCAAAAGGAATAAAGAGGTTTTTGATCAGTCAGTAGAGCTTGAACATGGACACGAGCTGATGAAATTCTCCCAGGTCCTTACTGCTATGGCATTCTCGCTCTTTGCTCAAGAGAATGTTGATGTTGCAGTCATTGAGGCTGGACTTGGTGGTGCACGGGATGCGACAAACATAATTTGCAGCTCTGGACTTGCTGCTGCAGTCATAACTTCGATTGGAGAGGAACACATGGCTGCCCTGGGTGGCTCTTTGGAAAGCATTGCAATGGCAAAGGCTGGAATAATTAAACGTGGTTGTCCAACAATTCTGGGTGGTTCATTCCTTCCAAGAATTGAGTACATTCTTCGCGATAAAGCATTGTCCATGTCTTCACCTGTAATAGCAGTCTCAGATCCTGGAAACAGAAGTACCATAAAAGGTGTAAACATGCTCAATGGAGGACTTTGCCAATGCTGCGACATAGTAATTCAAATAGACAATGAATTCGTCGAGTTATTGGATGTCAACCTCCGCATGCTTGGACGTCATCAACTTCAGAATGCAGCCACTGCAACTTGTGTTATTCTTACTCTCCGCAATCTAGGTTGGAGAATTTCGGATGCATCTATCAGGAGTGGACTAGAGCAAACATTCCTTATTGGTAGGAGTCATTTCTTAGCAGCCAAGGAAGCTGAGGTTCTTGGACTACCTGGAGCAACAATATTGCTGGATGGAGGTGCATTCATATTTCCATTGTGCTCATGACAATTTGGTCTGTTGGAACCTGATCATTGTGCTCATGCTGCGTCTTCTCTATCGTATTGTACTTAAAGATTTTTGGCAGTTTGATGTTCAGTTCATGGGCTCTCTTTTCACTGTTATTGGTAAATGTTTATAGTTGGTCTGCTGATGCCTGACGCTTGAAGGTTTGCAGAATATTGTAGATCAACTTAGTGTTTTCGACCTGCATCAACTGATATATATATATATTTGAGTTATGTTGTTCTGGATATTGGTGCTATGTCTTGCATTCTTTTAGACTGCATTTCTAATTGGTTCTTTAATTTTTTCACAGCACATACCAAAGACTCTGCTAAAGCATTAGTGGATACCATTCAAATGGCTTTCCCTGAGGCTCGATTGGCTCTTGTGATTGCAATGGCTAGTGATAAGAATCACGTTGATTTTGCCAGAGAATTCCTTCAAGGTGGAAAATTAGAGTGCGTTGTCTTAACTGAAGCCCACATTGGTGGAGGCAAATCAAGGACAACGTCAGCTGCTTTTCTAAGAGACTGCTGGATCCAAGCATCCATTGAATTGGGGATCCCTATTTCTTTAGAAACTAAAGATGCAGAAGTTTCCTTCACAAGCAAACTCGAAAACAGACCGGTATTGACTACTGAAACCTCGTTATTACACGCCATAAAAATTGCTGCTGAGATTCTTAAGCAAAGAACGAAAGGACAGCAAAGCCTTGTCGTAGTATCTGGCTCTCTGCATGCTGTTTCAATGGTGTTATCTTCTCTTCATTCTTGAAGTCTTTATTTGTAAAAGCACTTCTTTTGTATGTACTTTGAGCATAGTTCTTGAATATTGCAGATTATTTTCTTAGCATATTTCTATGAATTATCTGAAGTGTGGCATTAGTGGCAGGGAAAAGTAATGAAACAAACCTTACAAAGGGTAAGTTGTTATTGCTC

Coding sequence (CDS)

ATGAATGTCTTCAAATATCATCACCACTGTCGCCCACAGATTCTCGGAAGCCTTCTCCTAAATCATTTCATTGGTGAATGCCCTTCTATCAGTTCCCGAAATGGGTCCAAACAATGCTTCGCCACTCACTCAGAAGATCAACACATGACTGAATTCATGGAATATTTAGATTCCCTGAAGAACTACGAGAAATTGGGTGTGCCCAGAGGTGCAGGCACTGATTCTGATGATGGGTTTGACCTGGGAAGGATGAGAAGGCTGATGGAGCGACTGGGTAATCCACAATCCAAGTTTAAGGCAATTCACATTGCTGGAACGAAGGGAAAAGGGTCGATTGCGGCATTCCTTTCGAACATTTTACGAGCAGAGGGATACTCAGTTGGTTGTTATACTAGTCCTCATATTGAAACTTTAAGGGAACGTATTTCACTTGGAAGATCTGGAGAGATGGTCTCTGGGAAGGCACTAAATTCTCTTTTCAAAAGGAATAAAGAGGTTTTTGATCAGTCAGTAGAGCTTGAACATGGACACGAGCTGATGAAATTCTCCCAGGTCCTTACTGCTATGGCATTCTCGCTCTTTGCTCAAGAGAATGTTGATGTTGCAGTCATTGAGGCTGGACTTGGTGGTGCACGGGATGCGACAAACATAATTTGCAGCTCTGGACTTGCTGCTGCAGTCATAACTTCGATTGGAGAGGAACACATGGCTGCCCTGGGTGGCTCTTTGGAAAGCATTGCAATGGCAAAGGCTGGAATAATTAAACGTGGTTGTCCAACAATTCTGGGTGGTTCATTCCTTCCAAGAATTGAGTACATTCTTCGCGATAAAGCATTGTCCATGTCTTCACCTGTAATAGCAGTCTCAGATCCTGGAAACAGAAGTACCATAAAAGGTGTAAACATGCTCAATGGAGGACTTTGCCAATGCTGCGACATAGTAATTCAAATAGACAATGAATTCGTCGAGTTATTGGATGTCAACCTCCGCATGCTTGGACGTCATCAACTTCAGAATGCAGCCACTGCAACTTGTGTTATTCTTACTCTCCGCAATCTAGGTTGGAGAATTTCGGATGCATCTATCAGGAGTGGACTAGAGCAAACATTCCTTATTGGTAGGAGTCATTTCTTAGCAGCCAAGGAAGCTGAGGTTCTTGGACTACCTGGAGCAACAATATTGCTGGATGGAGGTGCATTCATATTTCCATTGTGCTCATGA

Protein sequence

MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGHELMKFSQVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGGAFIFPLCS

Homology

[Close]

BLAST of MELO3C026934.jh1 vs. NCBI nr
Match: XP_008465388.1 (PREDICTED: dihydrofolate synthetase isoform X1 [Cucumis melo])

HSP 1 Score: 1068 bits (2763), Expect = 0.0
Identity = 548/548 (100.00%), Postives = 548/548 (100.00%), Query Frame = 0


Query: 159 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 218
           MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 219 NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 278
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 279 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 338
           RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 339 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 398
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 399 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 458
           LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM
Sbjct: 241 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 300

Query: 459 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 518
           LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 519 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 578
           RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 420

Query: 579 VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP 638
           VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP
Sbjct: 421 VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP 480

Query: 639 ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVS 698
           ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVS
Sbjct: 481 ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVS 540

Query: 699 MVLSSLHS 706
           MVLSSLHS
Sbjct: 541 MVLSSLHS 548

[Close]

BLAST of MELO3C026934.jh1 vs. NCBI nr
Match: XP_004149457.1 (dihydrofolate synthetase isoform X2 [Cucumis sativus])

HSP 1 Score: 1002 bits (2591), Expect = 0.0
Identity = 513/548 (93.61%), Postives = 531/548 (96.90%), Query Frame = 0


Query: 159 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 218
           MNVFKYHHHCRPQILGSLLLNHFIGECPSISSR GSKQCFATHSEDQHMT+FMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 219 NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 278
           NYEKLGVPRG+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 279 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 338
           R EGYSVGCYTSPHIET+RERISLGRSG+MVSGKALNSLFKRNKEVFDQSVELE+G +SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 339 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 398
           FEVLTAMAFSLFAQE+VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240

Query: 399 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 458
           LESIA AKAGIIKRGCPTILGG FLPRIEYILRDKALSMSSPVI+ SDPGNRSTIKGVN+
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300

Query: 459 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 518
           LNGGL QCCDIVIQIDNEF+ELLDVNLRMLG HQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 519 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 578
           RSGLEQTFLIGRSHFLAA+EAEVLGLPGATILLDGAHTKDSAKAL+DTIQMAFPEA+LAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLAL 420

Query: 579 VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP 638
           V+AMASDKNHV FAREFLQGGKLE VVLTEA IGGGKSRTTSAAFLRDCWIQAS ELGIP
Sbjct: 421 VVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIP 480

Query: 639 ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVS 698
           ISLETKDAEV FTSKL NRPVLTTETSLLHAIKIAAEILKQRTKG+Q LVVVSGSLHAVS
Sbjct: 481 ISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQGLVVVSGSLHAVS 540

Query: 699 MVLSSLHS 706
           MVL+SLHS
Sbjct: 541 MVLASLHS 548

[Close]

BLAST of MELO3C026934.jh1 vs. NCBI nr
Match: XP_011651415.1 (dihydrofolate synthetase isoform X1 [Cucumis sativus] >KGN57917.2 hypothetical protein Csa_010822 [Cucumis sativus])

HSP 1 Score: 991 bits (2563), Expect = 0.0
Identity = 507/541 (93.72%), Postives = 524/541 (96.86%), Query Frame = 0


Query: 159 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 218
           MNVFKYHHHCRPQILGSLLLNHFIGECPSISSR GSKQCFATHSEDQHMT+FMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 219 NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 278
           NYEKLGVPRG+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 279 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 338
           R EGYSVGCYTSPHIET+RERISLGRSG+MVSGKALNSLFKRNKEVFDQSVELE+G +SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 339 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 398
           FEVLTAMAFSLFAQE+VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240

Query: 399 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 458
           LESIA AKAGIIKRGCPTILGG FLPRIEYILRDKALSMSSPVI+ SDPGNRSTIKGVN+
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300

Query: 459 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 518
           LNGGL QCCDIVIQIDNEF+ELLDVNLRMLG HQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 519 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 578
           RSGLEQTFLIGRSHFLAA+EAEVLGLPGATILLDGAHTKDSAKAL+DTIQMAFPEA+LAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLAL 420

Query: 579 VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP 638
           V+AMASDKNHV FAREFLQGGKLE VVLTEA IGGGKSRTTSAAFLRDCWIQAS ELGIP
Sbjct: 421 VVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIP 480

Query: 639 ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVS 698
           ISLETKDAEV FTSKL NRPVLTTETSLLHAIKIAAEILKQRTKG+Q LVVVSGSLHAVS
Sbjct: 481 ISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQGLVVVSGSLHAVS 540

[Close]

BLAST of MELO3C026934.jh1 vs. NCBI nr
Match: XP_038905573.1 (dihydrofolate synthetase [Benincasa hispida])

HSP 1 Score: 981 bits (2537), Expect = 0.0
Identity = 497/548 (90.69%), Postives = 521/548 (95.07%), Query Frame = 0


Query: 159 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 218
           M +FKYHHHCRPQILG+LLLNHF GECPSIS R GSKQCF+TH EDQHMTEFMEYLDSLK
Sbjct: 1   MKIFKYHHHCRPQILGTLLLNHFRGECPSISVRIGSKQCFSTHPEDQHMTEFMEYLDSLK 60

Query: 219 NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 278
           NYEKLGVP+GAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPKGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 279 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 338
           RAEGYS GCYTSPHIET+RERISLGRSGEMVSGKALNSLFKRNKE FDQSVELE+GRISH
Sbjct: 121 RAEGYSFGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180

Query: 339 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 398
           FEVLTAMAF LFAQENVD+AVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFLLFAQENVDLAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 399 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 458
           LESIA AKAGIIKRGCP ILGG FLP IE ILRDKALSMSSPVI+ S PGNRSTIKGV++
Sbjct: 241 LESIATAKAGIIKRGCPAILGGPFLPSIERILRDKALSMSSPVISASGPGNRSTIKGVSL 300

Query: 459 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 518
           LNGG+CQCCD+VIQID+EF+ELLDVNLRMLG HQLQNAATATCVIL LRNLGWRISDAS+
Sbjct: 301 LNGGICQCCDVVIQIDDEFIELLDVNLRMLGHHQLQNAATATCVILALRNLGWRISDASV 360

Query: 519 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 578
           RSGLEQTFL+GRSHFL AKEA VLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL
Sbjct: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 420

Query: 579 VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP 638
           V+AMASDK+H+ FAREFLQGGKLE +VLTEAHIGGGKSRTTSA FLRDCWIQAS ELGIP
Sbjct: 421 VVAMASDKDHIGFAREFLQGGKLESIVLTEAHIGGGKSRTTSAVFLRDCWIQASNELGIP 480

Query: 639 ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVS 698
           ISLETKDA VSFTSKLENRPVLTTETSLLHAIK+AAEILKQRT+G+Q LVVV+GSLHAVS
Sbjct: 481 ISLETKDAPVSFTSKLENRPVLTTETSLLHAIKVAAEILKQRTEGRQGLVVVTGSLHAVS 540

Query: 699 MVLSSLHS 706
           MVLS LHS
Sbjct: 541 MVLSFLHS 548

[Close]

BLAST of MELO3C026934.jh1 vs. NCBI nr
Match: KAA0050459.1 (dihydrofolate synthetase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 0


Query: 207 MTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKG 266
           MTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKG
Sbjct: 1   MTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKG 60

Query: 267 KGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFD 326
           KGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFD
Sbjct: 61  KGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFD 120

Query: 327 QSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITS 386
           QSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITS
Sbjct: 121 QSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITS 180

Query: 387 IGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSD 446
           IGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSD
Sbjct: 181 IGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSD 240

Query: 447 PGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTL 506
           PGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTL
Sbjct: 241 PGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTL 300

Query: 507 RNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDT 566
           RNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDT
Sbjct: 301 RNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDT 360

Query: 567 IQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRD 626
           IQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRD
Sbjct: 361 IQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRD 420

Query: 627 CWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQS 686
           CWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQS
Sbjct: 421 CWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQS 480

Query: 687 LVVVSGSLHAVSMVLSSLHS 706
           LVVVSGSLHAVSMVLSSLHS
Sbjct: 481 LVVVSGSLHAVSMVLSSLHS 500

[Close]

BLAST of MELO3C026934.jh1 vs. ExPASy Swiss-Prot
Match: F4JYE9 (Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 9.6e-159
Identity = 317/523 (60.61%), Postives = 388/523 (74.19%), Query Frame = 0


Query: 188 ISSRNGSKQCFATHSEDQHMTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLME 247
           IS+RN S     + +ED  + +F+ +L+SLKNYEK GVP+GAGTDSDDGFDLGRM+RLM 
Sbjct: 29  ISNRNLST---ISSTEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLML 88

Query: 248 RLGNPQSKFKAIHIAGTKGKGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGE 307
           RL NP  K+K +H+AGTKGKGS +AFLSNILRA GYSVGCY+SPHI +++ERIS   +GE
Sbjct: 89  RLRNPHYKYKVVHVAGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGE 148

Query: 308 MVSGKALNSLFKRNKEVFDQSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGA 367
            VS   LN LF   K + +QS++ E+G +SHFE+LT +AFSLF +ENVD+AVIEAGLGGA
Sbjct: 149 PVSASTLNDLFYSVKPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGA 208

Query: 368 RDATNIICSSGLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIE 427
           RDATN+I SS LAA+VIT+IGEEHMAALGGSLESIA AK+GIIK G P +LGG FLP IE
Sbjct: 209 RDATNVIESSNLAASVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIE 268

Query: 428 YILRDKALSMSSPVIAVSDPGNRSTIKGVNMLNG-GLCQCCDIVIQ---IDNEFVELLDV 487
            ILR KA S+SS VI  S+ G+ S+IKG+   NG GLCQ CDIVIQ    D   VEL DV
Sbjct: 269 GILRSKAASVSSSVILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDV 328

Query: 488 NLRMLGRHQLQNAATATCVILTLRNLG-WRISDASIRSGLEQTFLIGRSHFLAAKEAEVL 547
           NLRMLG HQLQNA TATCV L LR+ G  R++D +IR GLE T L+GRS FL  KEAE L
Sbjct: 329 NLRMLGHHQLQNAVTATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETL 388

Query: 548 GLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLE 607
            LPGAT+LLDGAHTK+SA+AL + I+  FPE RL  V+AMASDK+HV FA+E L G K E
Sbjct: 389 LLPGATVLLDGAHTKESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPE 448

Query: 608 CVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIPISLETKDAEVSFTSKLENRPVLTT 667
            V+LTEA IGGGK R+T ++ L++ WI+A+ ELG      ++  E S     EN+ V   
Sbjct: 449 AVILTEADIGGGKIRSTESSVLKESWIKAADELG------SRSMEAS-----ENKTV--- 508

Query: 668 ETSLLHAIKIAAEILKQRTKGQQS-LVVVSGSLHAVSMVLSSL 705
               L ++K+A +IL   T    S +V+V+GSLH VS VL+SL
Sbjct: 509 ----LGSLKLAYKILSDDTTSSDSGMVIVTGSLHIVSSVLASL 528

[Close]

BLAST of MELO3C026934.jh1 vs. ExPASy Swiss-Prot
Match: Q05865 (Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=folC PE=3 SV=2)

HSP 1 Score: 179.1 bits (453), Expect = 1.7e-43
Identity = 122/351 (34.76%), Postives = 188/351 (53.56%), Query Frame = 0


Query: 239 LGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNILRAEGYSVGCYTSPHIETLRE 298
           LGRM++LM RLG+P+ K +A H+AGT GKGS  AF+ ++L+  GY+VG +TSP+I T  E
Sbjct: 24  LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNE 83

Query: 299 RISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISHFEVLTAMAFSLFAQ-ENVDV 358
           RIS+  +G  +S +   +L  + K   +   + E+G+ + FE++TA AF  FA+   VD 
Sbjct: 84  RISV--NGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDF 143

Query: 359 AVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKRGCPTI 418
            + E GLGG  D+TN++        VITSIG +HM  LG ++E IA  KAGIIK G P I
Sbjct: 144 VIFETGLGGRFDSTNVV---EPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIP-I 203

Query: 419 LGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNMLNGGLCQCCDIVIQIDNEF 478
           +     P    ++R +A   ++P  ++ D      I     L  G         + + + 
Sbjct: 204 VTAVTQPEALQVIRHEAERHAAPFQSLHD---ACVIFNEEALPAGE----QFSFKTEEKC 263

Query: 479 VELLDVNLRMLGRHQLQNAATATCVI--LTLRNLGWRISDASIRSGLEQTFLIGRSHFLA 538
            E  D+   ++G HQ QNAA +      L   N+   ISD ++RSGL +    GR   + 
Sbjct: 264 YE--DIRTSLIGTHQRQNAALSILAAEWLNKENIA-HISDEALRSGLVKAAWPGRLELVQ 323

Query: 539 AKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVIAMASDK 587
                        + LDGAH ++  + L +T++  F  +R+++V +   DK
Sbjct: 324 EH---------PPVYLDGAHNEEGVEKLAETMKQRFANSRISVVFSALKDK 349

[Close]

BLAST of MELO3C026934.jh1 vs. ExPASy Swiss-Prot
Match: P15925 (Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 7.6e-31
Identity = 109/387 (28.17%), Postives = 186/387 (48.06%), Query Frame = 0


Query: 208 TEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGK 267
           TE + Y+ S     K G             D  R+  L+  LGNPQ + + IH+ GT GK
Sbjct: 4   TETVAYIHSFPRLAKTG-------------DHRRILTLLHALGNPQQQGRYIHVTGTNGK 63

Query: 268 GSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQ 327
           GS A  ++++L A G +VG YTSP I    ERI +    E +   AL +     +   ++
Sbjct: 64  GSAANAIAHVLEASGLTVGLYTSPFIMRFNERIMIDH--EPIPDAALVNAVAFVRAALER 123

Query: 328 SVELEHG-RISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITS 387
             + +    ++ FE +TA+ +  F Q  VDVAVIE G+GG  D+TN+I       +V+T 
Sbjct: 124 LQQQQADFNVTEFEFITALGYWYFRQRQVDVAVIEVGIGGDTDSTNVITP---VVSVLTE 183

Query: 388 IGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSD 447
           +  +H   LG ++ +IA  KAGIIKRG P ++ G+ +P    ++  K  +  S  +    
Sbjct: 184 VALDHQKLLGHTITAIAKHKAGIIKRGIP-VVTGNLVPDAAAVVAAKVATTGSQWLRFDR 243

Query: 448 PGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATA-TCVILT 507
             +    K    L+G   +        +++   + D+ + ++G +Q +N A A     + 
Sbjct: 244 DFSVPKAK----LHGWGQR-----FTYEDQDGRISDLEVPLVGDYQQRNMAIAIQTAKVY 303

Query: 508 LRNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVD 567
            +   W ++  +IR GL        SH+ A  E ++   P   I++DGAH  D    L+ 
Sbjct: 304 AKQTEWPLTPQNIRQGL------AASHWPARLE-KISDTP--LIVIDGAHNPDGINGLIT 352

Query: 568 TIQMAFPEARLALVIAMASDKNHVDFA 593
            ++  F +  + ++  + +DK++   A
Sbjct: 364 ALKQLFSQP-ITVIAGILADKDYAAMA 352

[Close]

BLAST of MELO3C026934.jh1 vs. ExPASy Swiss-Prot
Match: D4GW05 (Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=folCP PE=3 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.7e-30
Identity = 109/349 (31.23%), Postives = 168/349 (48.14%), Query Frame = 0


Query: 242 MRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNILRAEGYSVGCYTSPHIETLRERIS 301
           +RRL+  LG+P      + +AG+ GKGS A  +  +LR  G  VG YTSPH + +RER+ 
Sbjct: 25  IRRLLSHLGDPHEGVSFVQVAGSNGKGSTARMVDAMLRESGAHVGLYTSPHFDDVRERVR 84

Query: 302 LGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIE 361
           +   G  +   AL++     K    +    +   ++ FE +TA+A   F +  VDVAV+E
Sbjct: 85  V--DGRKIPKSALSAFVAEAKPYLVERA-ADGEPLTFFETVTALALWYFDRAGVDVAVLE 144

Query: 362 AGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKRGCPTILG-- 421
            G+GG  DAT+ +      A+ +T++  EH A LG ++  IA  KA +     P + G  
Sbjct: 145 VGMGGELDATSAV---DPVASAVTNVSLEHTAVLGDTVAEIAKTKAAVAPADAPLVTGTT 204

Query: 422 GSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVE 481
           G  L     ++R++A      V+ V D  +  +   V +  GG     +  + ++ +  E
Sbjct: 205 GEALS----VIREEA----GDVLTVGD-ADADSDADVRVSYGGRVNHQEAAVTVETD-AE 264

Query: 482 LLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAKEA 541
            LDV + +LG +Q +NA  A  +   +R     I   +I  GL      GR         
Sbjct: 265 TLDVRIPLLGAYQARNAGIAVSLARQVRP---DIDAEAIHRGLRNAHWPGR--------F 324

Query: 542 EVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVIAMASDKNH 589
           EV+G    T++LDGAH  D A A V T+   F    L LV     DK+H
Sbjct: 325 EVMGTE-PTVVLDGAHNPD-ACAQVATVLDEFDYDDLHLVYGAMHDKDH 344

[Close]

BLAST of MELO3C026934.jh1 vs. ExPASy Swiss-Prot
Match: Q12676 (Dihydrofolate synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=FOL3 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 2.2e-30
Identity = 106/372 (28.49%), Postives = 186/372 (50.00%), Query Frame = 0


Query: 239 LGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNILRAEGYSVGCYTSPHIETLRE 298
           L R+ +L+E LGNPQ+  + +HIAGT GKGS+  +LS++L+ + Y +G +T+PH+  + +
Sbjct: 7   LSRITKLLEHLGNPQNSLRVLHIAGTNGKGSVCTYLSSVLQQKSYQIGKFTTPHLVHVTD 66

Query: 299 RISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHG-RISHFEVLTAMAFSLFAQENVDV 358
            I+       ++ K +     +N  +  +++   H  + + FE+LT  AF  F       
Sbjct: 67  SIT-------INNKPIPLERYQNIRLQLEALNKSHSLKCTEFELLTCTAFKYFYDVQCQW 126

Query: 359 AVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKRGCP-T 418
            VIE GLGG  DATN+I  +  A   IT I  +H + LG +L  I+  KAGII  G P T
Sbjct: 127 CVIEVGLGGRLDATNVIPGANKACCGITKISLDHESFLGNTLSEISKEKAGIITEGVPFT 186

Query: 419 ILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNMLNGGLCQCCDIVIQIDNE 478
           ++ G+    +  +++++  ++ S  ++V+D    S + G NM++     C D+       
Sbjct: 187 VIDGTNEASVINVVKERCKALGSE-LSVTD----SQLNG-NMIDTNSWGCFDL------- 246

Query: 479 FVELLDVNLRMLGRHQLQNAATATCVILTLR-NLGWRISDASIRSGLEQTFLIGRSHFLA 538
                   L + G +Q+ N   A  ++  L+ N    I+   + + L +    GR + + 
Sbjct: 247 ------AKLPLNGEYQIFNLRVAMGMLDYLQMNELIDITKNEVSTRLAKVDWPGRLYRMD 306

Query: 539 AKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVIAMASDKNHVDFAREF 598
            +  +V       IL+DGAH   +A  LV  ++  +    L  V+A+   KN     +  
Sbjct: 307 YRFDKVSNRT-VPILMDGAHNGSAAVELVKYLRKEYGNQPLTFVMAVTHGKNLEPLLQPL 349

Query: 599 LQGGKLECVVLT 608
           L+   ++ V+LT
Sbjct: 367 LR--PIDQVILT 349

[Close]

BLAST of MELO3C026934.jh1 vs. ExPASy TrEMBL
Match: A0A1S3CNR7 (dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3 SV=1)

HSP 1 Score: 1068 bits (2763), Expect = 0.0
Identity = 548/548 (100.00%), Postives = 548/548 (100.00%), Query Frame = 0


Query: 159 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 218
           MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 219 NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 278
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 279 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 338
           RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 339 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 398
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 399 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 458
           LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM
Sbjct: 241 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 300

Query: 459 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 518
           LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 519 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 578
           RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 420

Query: 579 VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP 638
           VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP
Sbjct: 421 VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP 480

Query: 639 ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVS 698
           ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVS
Sbjct: 481 ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVS 540

Query: 699 MVLSSLHS 706
           MVLSSLHS
Sbjct: 541 MVLSSLHS 548

[Close]

BLAST of MELO3C026934.jh1 vs. ExPASy TrEMBL
Match: A0A0A0L7A4 (Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G354540 PE=3 SV=1)

HSP 1 Score: 1002 bits (2591), Expect = 0.0
Identity = 513/548 (93.61%), Postives = 531/548 (96.90%), Query Frame = 0


Query: 159 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 218
           MNVFKYHHHCRPQILGSLLLNHFIGECPSISSR GSKQCFATHSEDQHMT+FMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 219 NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 278
           NYEKLGVPRG+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 279 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 338
           R EGYSVGCYTSPHIET+RERISLGRSG+MVSGKALNSLFKRNKEVFDQSVELE+G +SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 339 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 398
           FEVLTAMAFSLFAQE+VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240

Query: 399 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 458
           LESIA AKAGIIKRGCPTILGG FLPRIEYILRDKALSMSSPVI+ SDPGNRSTIKGVN+
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300

Query: 459 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 518
           LNGGL QCCDIVIQIDNEF+ELLDVNLRMLG HQLQNAATATCVILTLRNLGWRISDASI
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 519 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 578
           RSGLEQTFLIGRSHFLAA+EAEVLGLPGATILLDGAHTKDSAKAL+DTIQMAFPEA+LAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLAL 420

Query: 579 VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP 638
           V+AMASDKNHV FAREFLQGGKLE VVLTEA IGGGKSRTTSAAFLRDCWIQAS ELGIP
Sbjct: 421 VVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIP 480

Query: 639 ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVS 698
           ISLETKDAEV FTSKL NRPVLTTETSLLHAIKIAAEILKQRTKG+Q LVVVSGSLHAVS
Sbjct: 481 ISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQGLVVVSGSLHAVS 540

Query: 699 MVLSSLHS 706
           MVL+SLHS
Sbjct: 541 MVLASLHS 548

[Close]

BLAST of MELO3C026934.jh1 vs. ExPASy TrEMBL
Match: A0A5A7UAE3 (Dihydrofolate synthetase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00720 PE=3 SV=1)

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 0


Query: 207 MTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKG 266
           MTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKG
Sbjct: 1   MTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKG 60

Query: 267 KGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFD 326
           KGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFD
Sbjct: 61  KGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFD 120

Query: 327 QSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITS 386
           QSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITS
Sbjct: 121 QSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITS 180

Query: 387 IGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSD 446
           IGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSD
Sbjct: 181 IGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSD 240

Query: 447 PGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTL 506
           PGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTL
Sbjct: 241 PGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTL 300

Query: 507 RNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDT 566
           RNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDT
Sbjct: 301 RNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDT 360

Query: 567 IQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRD 626
           IQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRD
Sbjct: 361 IQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRD 420

Query: 627 CWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQS 686
           CWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQS
Sbjct: 421 CWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQS 480

Query: 687 LVVVSGSLHAVSMVLSSLHS 706
           LVVVSGSLHAVSMVLSSLHS
Sbjct: 481 LVVVSGSLHAVSMVLSSLHS 500

[Close]

BLAST of MELO3C026934.jh1 vs. ExPASy TrEMBL
Match: A0A5D3BTU4 (Dihydrofolate synthetase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11G00160 PE=3 SV=1)

HSP 1 Score: 959 bits (2478), Expect = 0.0
Identity = 497/500 (99.40%), Postives = 497/500 (99.40%), Query Frame = 0


Query: 207 MTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKG 266
           MTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKG
Sbjct: 1   MTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKG 60

Query: 267 KGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFD 326
           KGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFD
Sbjct: 61  KGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFD 120

Query: 327 QSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITS 386
           QSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITS
Sbjct: 121 QSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITS 180

Query: 387 IGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSD 446
           IGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIA SD
Sbjct: 181 IGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAASD 240

Query: 447 PGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTL 506
           PGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTL
Sbjct: 241 PGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTL 300

Query: 507 RNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDT 566
           RNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPG TILLDGAHTKDSAKALVDT
Sbjct: 301 RNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGVTILLDGAHTKDSAKALVDT 360

Query: 567 IQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRD 626
           IQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLE VVLTEAHIGGGKSRTTSAAFLRD
Sbjct: 361 IQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLESVVLTEAHIGGGKSRTTSAAFLRD 420

Query: 627 CWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQS 686
           CWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQS
Sbjct: 421 CWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQS 480

Query: 687 LVVVSGSLHAVSMVLSSLHS 706
           LVVVSGSLHAVSMVLSSLHS
Sbjct: 481 LVVVSGSLHAVSMVLSSLHS 500

[Close]

BLAST of MELO3C026934.jh1 vs. ExPASy TrEMBL
Match: A0A6J1CJG1 (dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011557 PE=3 SV=1)

HSP 1 Score: 939 bits (2426), Expect = 0.0
Identity = 484/548 (88.32%), Postives = 511/548 (93.25%), Query Frame = 0


Query: 159 MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 218
           MNVFKYH   RPQILG LLLN  +GE PSIS R GSKQCFATHSED  +TEFMEYLDSLK
Sbjct: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60

Query: 219 NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 278
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 279 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 338
           RAEGYSVGCYTSPHIET+RERISLGRSGEMVSGKALNSLFKRNKE  DQSVE E+GRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180

Query: 339 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 398
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 399 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 458
           LESIAMAKAGIIK GCPTILGG FLP IE IL DKALSMSS VI+ S PG+RSTIKGV++
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300

Query: 459 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 518
           L+G LCQCCDIVIQIDNEF+ELLDVNLRMLGRHQLQNAATATC ILTLRNLGWRISD+SI
Sbjct: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360

Query: 519 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 578
           ++GLEQTFL+GRSHFL+AKEA VLGLPGATILLDGAHTKDSAKALVDTIQM FPEARLAL
Sbjct: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420

Query: 579 VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP 638
           V+AMASDK+HV FAREFLQGGKLE ++LTEA+IGGGKSRTTSAA LRDCWIQA+ ELGIP
Sbjct: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480

Query: 639 ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVS 698
           ISLETKDA VS TSKLE+RP+LTTETSLLHA+KIAAEILKQRT+G+  L VV+GSLHAVS
Sbjct: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS 540

Query: 699 MVLSSLHS 706
           MVLSSLHS
Sbjct: 541 MVLSSLHS 548

[Close]

BLAST of MELO3C026934.jh1 vs. TAIR 10
Match: AT5G41480.1 (Folylpolyglutamate synthetase family protein )

HSP 1 Score: 562.0 bits (1447), Expect = 6.8e-160
Identity = 317/523 (60.61%), Postives = 388/523 (74.19%), Query Frame = 0


Query: 188 ISSRNGSKQCFATHSEDQHMTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLME 247
           IS+RN S     + +ED  + +F+ +L+SLKNYEK GVP+GAGTDSDDGFDLGRM+RLM 
Sbjct: 29  ISNRNLST---ISSTEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLML 88

Query: 248 RLGNPQSKFKAIHIAGTKGKGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGE 307
           RL NP  K+K +H+AGTKGKGS +AFLSNILRA GYSVGCY+SPHI +++ERIS   +GE
Sbjct: 89  RLRNPHYKYKVVHVAGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGE 148

Query: 308 MVSGKALNSLFKRNKEVFDQSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGA 367
            VS   LN LF   K + +QS++ E+G +SHFE+LT +AFSLF +ENVD+AVIEAGLGGA
Sbjct: 149 PVSASTLNDLFYSVKPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGA 208

Query: 368 RDATNIICSSGLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIE 427
           RDATN+I SS LAA+VIT+IGEEHMAALGGSLESIA AK+GIIK G P +LGG FLP IE
Sbjct: 209 RDATNVIESSNLAASVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIE 268

Query: 428 YILRDKALSMSSPVIAVSDPGNRSTIKGVNMLNG-GLCQCCDIVIQ---IDNEFVELLDV 487
            ILR KA S+SS VI  S+ G+ S+IKG+   NG GLCQ CDIVIQ    D   VEL DV
Sbjct: 269 GILRSKAASVSSSVILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDV 328

Query: 488 NLRMLGRHQLQNAATATCVILTLRNLG-WRISDASIRSGLEQTFLIGRSHFLAAKEAEVL 547
           NLRMLG HQLQNA TATCV L LR+ G  R++D +IR GLE T L+GRS FL  KEAE L
Sbjct: 329 NLRMLGHHQLQNAVTATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETL 388

Query: 548 GLPGATILLDGAHTKDSAKALVDTIQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLE 607
            LPGAT+LLDGAHTK+SA+AL + I+  FPE RL  V+AMASDK+HV FA+E L G K E
Sbjct: 389 LLPGATVLLDGAHTKESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPE 448

Query: 608 CVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIPISLETKDAEVSFTSKLENRPVLTT 667
            V+LTEA IGGGK R+T ++ L++ WI+A+ ELG      ++  E S     EN+ V   
Sbjct: 449 AVILTEADIGGGKIRSTESSVLKESWIKAADELG------SRSMEAS-----ENKTV--- 508

Query: 668 ETSLLHAIKIAAEILKQRTKGQQS-LVVVSGSLHAVSMVLSSL 705
               L ++K+A +IL   T    S +V+V+GSLH VS VL+SL
Sbjct: 509 ----LGSLKLAYKILSDDTTSSDSGMVIVTGSLHIVSSVLASL 528

[Close]

BLAST of MELO3C026934.jh1 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 118.6 bits (296), Expect = 2.0e-26
Identity = 116/381 (30.45%), Postives = 169/381 (44.36%), Query Frame = 0


Query: 199 ATHSEDQHMTEFMEYLDSLKN-YEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFK 258
           AT  + +    +   LD+L +   K G  R A  +    F L      +  L +  S+ K
Sbjct: 2   ATEDDGELSARYQNTLDALSSLITKRG--RLASNNQSHRFRLLFHYLKVLELEDAVSQMK 61

Query: 259 AIHIAGTKGKGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALN-- 318
            IH+AGTKGKGS   F  +ILR  G   G +TSPH+  +RER  L    E+   K +N  
Sbjct: 62  IIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL-NGIEISQEKFVNYF 121

Query: 319 -SLFKRNKEVFDQSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNII 378
              F + KE     V +     ++F  L  +AF +F  E VDV ++E GLGG  DATN+I
Sbjct: 122 WCCFHKLKEKTSNEVPMP----TYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVI 181

Query: 379 CSSGLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKA 438
               +    I+S+G +HM  LG +L  IA  KAGI K G P                  A
Sbjct: 182 QKPVVCG--ISSLGYDHMEILGYTLAEIAAEKAGIFKSGVP------------------A 241

Query: 439 LSMSSPVIAVSDPGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLR--MLGRHQ 498
            +++ P  A+     +++   VN+                 + VE LD + R  + G HQ
Sbjct: 242 FTVAQPDEAMRVLNEKASKLEVNL-----------------QVVEPLDSSQRLGLQGEHQ 301

Query: 499 LQNAATATCVILT-LRNLGWRISDA---------SIRSGLEQTFLIGRSHFLAAKEAEVL 558
             NA  A  +  T L+ +G    +             SGL   +L+GR+  +   E    
Sbjct: 302 YLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMIVPDSE---- 333

Query: 559 GLPGATI-LLDGAHTKDSAKA 563
            LP   +  LDGAH+ +S +A
Sbjct: 362 -LPEEIVYYLDGAHSPESMEA 333

[Close]

BLAST of MELO3C026934.jh1 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 117.9 bits (294), Expect = 3.4e-26
Identity = 117/385 (30.39%), Postives = 169/385 (43.90%), Query Frame = 0


Query: 199 ATHSEDQHMTEFMEYLDSLKN-YEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFK 258
           AT  + +    +   LD+L +   K G  R A  +    F L      +  L +  S+ K
Sbjct: 2   ATEDDGELSARYQNTLDALSSLITKRG--RLASNNQSHRFRLLFHYLKVLELEDAVSQMK 61

Query: 259 AIHIAGTKGKGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALN-- 318
            IH+AGTKGKGS   F  +ILR  G   G +TSPH+  +RER  L    E+   K +N  
Sbjct: 62  IIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL-NGIEISQEKFVNYF 121

Query: 319 -SLFKRNKEVFDQSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNI- 378
              F + KE     V +     ++F  L  +AF +F  E VDV ++E GLGG  DATNI 
Sbjct: 122 WCCFHKLKEKTSNEVPMP----TYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNIQ 181

Query: 379 ---ICSSGLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYIL 438
              +C        I+S+G +HM  LG +L  IA  KAGI K G P               
Sbjct: 182 KPVVCG-------ISSLGYDHMEILGYTLAEIAAEKAGIFKSGVP--------------- 241

Query: 439 RDKALSMSSPVIAVSDPGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLR--ML 498
              A +++ P  A+     +++   VN+                 + VE LD + R  + 
Sbjct: 242 ---AFTVAQPDEAMRVLNEKASKLEVNL-----------------QVVEPLDSSQRLGLQ 301

Query: 499 GRHQLQNAATATCVILT-LRNLGWRISDA---------SIRSGLEQTFLIGRSHFLAAKE 558
           G HQ  NA  A  +  T L+ +G    +             SGL   +L+GR+  +   E
Sbjct: 302 GEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMIVPDSE 332

Query: 559 AEVLGLPGATI-LLDGAHTKDSAKA 563
                LP   +  LDGAH+ +S +A
Sbjct: 362 -----LPEEIVYYLDGAHSPESMEA 332

[Close]

BLAST of MELO3C026934.jh1 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 106.3 bits (264), Expect = 1.0e-22
Identity = 66/165 (40.00%), Postives = 91/165 (55.15%), Query Frame = 0


Query: 255 KFKAIHIAGTKGKGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKAL 314
           K   IH+AGTKGKGS   F  +I+R  G+  G +TSPH+  +RER  L   G  +S +  
Sbjct: 111 KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRL--DGVDISEEKF 170

Query: 315 NSLF----KRNKEVFDQSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDA 374
              F     R KE  ++ + +     ++F  L  +AF +FA E VD A++E GLGG  DA
Sbjct: 171 LGYFWWCYNRLKERTNEEIPMP----TYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDA 230

Query: 375 TNIICSSGLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKRGCP 416
           TN +    +    I+S+G +HM  LG +L  IA  KAGI K G P
Sbjct: 231 TNAVQKPVVCG--ISSLGYDHMEILGDTLGKIAGEKAGIFKLGVP 267

[Close]

BLAST of MELO3C026934.jh1 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 106.3 bits (264), Expect = 1.0e-22
Identity = 66/165 (40.00%), Postives = 91/165 (55.15%), Query Frame = 0


Query: 255 KFKAIHIAGTKGKGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKAL 314
           K   IH+AGTKGKGS   F  +I+R  G+  G +TSPH+  +RER  L   G  +S +  
Sbjct: 53  KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRL--DGVDISEEKF 112

Query: 315 NSLF----KRNKEVFDQSVELEHGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDA 374
              F     R KE  ++ + +     ++F  L  +AF +FA E VD A++E GLGG  DA
Sbjct: 113 LGYFWWCYNRLKERTNEEIPMP----TYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDA 172

Query: 375 TNIICSSGLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKRGCP 416
           TN +    +    I+S+G +HM  LG +L  IA  KAGI K G P
Sbjct: 173 TNAVQKPVVCG--ISSLGYDHMEILGDTLGKIAGEKAGIFKLGVP 209

The following BLAST results are available for this feature:

BLAST of MELO3C026934.jh1 vs. NCBI nr


Match Name	Identity	Description
XP_008465388.1	100.00	PREDICTED: dihydrofolate synthetase isoform X1 [Cucumis melo]	[more]
XP_004149457.1	93.61	dihydrofolate synthetase isoform X2 [Cucumis sativus]	[more]
XP_011651415.1	93.72	dihydrofolate synthetase isoform X1 [Cucumis sativus] >KGN57917.2 hypothetical p...	[more]
XP_038905573.1	90.69	dihydrofolate synthetase [Benincasa hispida]	[more]
KAA0050459.1	100.00	dihydrofolate synthetase isoform X1 [Cucumis melo var. makuwa]	[more]

BLAST of MELO3C026934.jh1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JYE9	9.6e-159	60.61	Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1	[more]
Q05865	1.7e-43	34.76	Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain ...	[more]
P15925	7.6e-31	28.17	Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1	[more]
D4GW05	1.7e-30	31.23	Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Ha...	[more]
Q12676	2.2e-30	28.49	Dihydrofolate synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	[more]

BLAST of MELO3C026934.jh1 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A1S3CNR7	100.00	dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3...	[more]
A0A0A0L7A4	93.61	Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G35454...	[more]
A0A5A7UAE3	100.00	Dihydrofolate synthetase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6...	[more]
A0A5D3BTU4	99.40	Dihydrofolate synthetase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A6J1CJG1	88.32	dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110115...	[more]

BLAST of MELO3C026934.jh1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41480.1	6.8e-160	60.61	Folylpolyglutamate synthetase family protein	[more]
AT3G55630.3	2.0e-26	30.45	DHFS-FPGS homolog D	[more]
AT3G55630.2	3.4e-26	30.39	DHFS-FPGS homolog D	[more]
AT5G05980.1	1.0e-22	40.00	DHFS-FPGS homolog B	[more]
AT5G05980.2	1.0e-22	40.00	DHFS-FPGS homolog B	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001645	Folylpolyglutamate synthetase	TIGRFAM	TIGR01499	TIGR01499	coord: 81..398 e-value: 1.8E-73 score: 246.2
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 46..398
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	3.40.1190.10		coord: 38..370 e-value: 2.4E-82 score: 278.3
IPR036565	Mur-like, catalytic domain superfamily	SUPERFAMILY	53623	MurD-like peptide ligases, catalytic domain	coord: 78..370
IPR013221	Mur ligase, central	PFAM	PF08245	Mur_ligase_M	coord: 103..346 e-value: 4.3E-9 score: 36.6
None	No IPR available	PIRSR	PIRSR038895-2	PIRSR038895-2	coord: 32..383 e-value: 2.7E-48 score: 162.6
None	No IPR available	PIRSR	PIRSR038895-1	PIRSR038895-1	coord: 32..383 e-value: 2.7E-48 score: 162.6
None	No IPR available	PANTHER	PTHR11136:SF0	DIHYDROFOLATE SYNTHETASE-RELATED	coord: 46..398
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01011	FOLYLPOLYGLU_SYNT_1	coord: 101..124
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01012	FOLYLPOLYGLU_SYNT_2	coord: 203..218

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C026934.jh1.t3	MELO3C026934.jh1.t3	mRNA
MELO3C026934.jh1.t2	MELO3C026934.jh1.t2	mRNA
MELO3C026934.jh1.t1	MELO3C026934.jh1.t1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0004326	tetrahydrofolylpolyglutamate synthase activity