Homology
BLAST of MELO3C026085 vs. ExPASy Swiss-Prot
Match:
F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)
HSP 1 Score: 852.8 bits (2202), Expect = 2.9e-246
Identity = 440/769 (57.22%), Postives = 542/769 (70.48%), Query Frame = 0
Query: 5 ISILIFFSFLLLI----SPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFL 64
+S+ S LLL+ SPA A KKSY+V LGSH+H +++ + V SH L SF+
Sbjct: 16 MSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFV 75
Query: 65 RSDEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMH 124
S E AK++IFYSYK+HINGFAA LD+ +A +A HP+V +V PNK + L+TTHSW FM
Sbjct: 76 GSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFML 135
Query: 125 LEKNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK-------- 184
L KNGVV SS W A +G+D IIANLDTGVWPESKSF + G G P++WK
Sbjct: 136 LAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGY-GAVPARWKGRCHKDVP 195
Query: 185 ------GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVF 244
GA+YFNKGYL Y + + ++ + RD+DGHGSHTLSTAAGN+V GA+VF
Sbjct: 196 CNRKLIGARYFNKGYLAY-----TGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVF 255
Query: 245 GSGIGTAKGGSPKARVAAYKVCWPFEQGG-CFDADITEAFDHAIHDGVDVLSLSLGGDPI 304
G G GTA GGSPKARVAAYKVCWP G CFDADI A + AI DGVDVLS S+GGD
Sbjct: 256 GIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAG 315
Query: 305 KYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVL 364
Y D IAI SFHAVK G+ VVC+ GNSGP T SN APW++TVGAS++DREF A V L
Sbjct: 316 DYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVEL 375
Query: 365 QNGHRFMGSSHSKGLTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCL 424
+NG F G+S SK L K+Y LI+ A A N N DA+LCK +LD KVKGKILVCL
Sbjct: 376 KNGQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCL 435
Query: 425 RGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSA 484
RG+ AR+DKG QAA AGA GM+LCNDK SG+ I+ D H+LPAS I+Y+DG+ L SY++S
Sbjct: 436 RGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSST 495
Query: 485 RNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPT 544
++P GY+ P A +NTKPAP MA FSSRGPNTI+P I+KPD+TAPGVNIIAAF+EA PT
Sbjct: 496 KDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPT 555
Query: 545 RDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNP 604
SDNR TPF T SGTSMSCPH++G+VGLL+ LHP WSP+AI+SAIMT+S+ R+N P
Sbjct: 556 DLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKP 615
Query: 605 MIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSD 664
M+D A PF+YGSGH+ P A PGLVYDL+ DYL+FLCA GY+ ++ F++
Sbjct: 616 MVDE---SFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAE 675
Query: 665 EP-FKCPPASSVLNLNYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKP 724
+P + C +++L+ NYPSI V NL GS++VTRKLKNVG P Y A+ P GV VSV+P
Sbjct: 676 DPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEP 735
Query: 725 RFLKFERVGEEKSFELTL--AGVVPKDRVGYGALIWSDGRHVVRSPIVV 752
+ L F + GE K F++TL V P V +G L W+D H VRSPIVV
Sbjct: 736 KQLTFNKTGEVKIFQMTLRPLPVTPSGYV-FGELTWTDSHHYVRSPIVV 774
BLAST of MELO3C026085 vs. ExPASy Swiss-Prot
Match:
Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)
HSP 1 Score: 761.5 bits (1965), Expect = 8.8e-219
Identity = 400/775 (51.61%), Postives = 523/775 (67.48%), Query Frame = 0
Query: 11 FSFLLLI------SPAIAAKK---SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLR 70
FSFLLL+ S I A K SYVV G+HSH E+TE + V ++H+ LGSF
Sbjct: 7 FSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTG 66
Query: 71 SDEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHL 130
S E+A D+IFYSY KHINGFAA LD + A ++ HPEV +V PNK L+TT SW+F+ L
Sbjct: 67 SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 126
Query: 131 EKNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK--------- 190
E N VP SS WR A+FG+D IIANLDTGVWPESKSF + G+ GP PS+WK
Sbjct: 127 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDA 186
Query: 191 ---------GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGA 250
GA+YFNKGY + NS+ D S RD DGHGSHTLSTAAG++V G
Sbjct: 187 TFHCNRKLIGARYFNKGYAAAVGHLNSSFD------SPRDLDGHGSHTLSTAAGDFVPGV 246
Query: 251 SVFGSGIGTAKGGSPKARVAAYKVCWPFEQGG-CFDADITEAFDHAIHDGVDVLSLSLGG 310
S+FG G GTAKGGSP+ARVAAYKVCWP +G C+DAD+ AFD AIHDG DV+S+SLGG
Sbjct: 247 SIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGG 306
Query: 311 DPIKYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAP 370
+P + DS+AI SFHA KK I VVC+ GNSGP T SN APW +TVGAST+DREF +
Sbjct: 307 EPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASN 366
Query: 371 VVLQNGHRFMGSS-HSKGLTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKI 430
+VL NG + G S S L K YP++ AKA NA+ DA LCK +LD K KGKI
Sbjct: 367 LVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKI 426
Query: 431 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSY 490
LVCLRG+ R++KG+ AL G +GM+L N ++G+ ++ D H+LPA+ + +D + Y
Sbjct: 427 LVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRY 486
Query: 491 INSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEA 550
I+ + P+ ++ P + KPAP MA FSS+GP+ ++P+I+KPD+TAPGV++IAA++ A
Sbjct: 487 ISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGA 546
Query: 551 ISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDN 610
+SPT + D R F +SGTSMSCPH++G+ GLL+ +P WSP+AI+SAIMT++ + D+
Sbjct: 547 VSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDD 606
Query: 611 TLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIR 670
P+ + ++ ATPF++G+GH+ P A++PGLVYDL DYL FLC+ GY+ I
Sbjct: 607 IPGPIQNATNM---KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQIS 666
Query: 671 AFSDEPFKC-PPASSVLNLNYPSIGVQNLKGS-VSVTRKLKNVGSPGVYRAQILHPNGVV 730
FS F C P S++NLNYPSI V NL S V+V+R +KNVG P +Y ++ +P GV
Sbjct: 667 VFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVY 726
Query: 731 VSVKPRFLKFERVGEEKSFELTLA---GVVPKDRVGYGALIWSDGRHVVRSPIVV 752
V+VKP L F +VGE+K+F++ L G V K V +G L+WSD +H VRSPIVV
Sbjct: 727 VAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYV-FGELVWSDKKHRVRSPIVV 770
BLAST of MELO3C026085 vs. ExPASy Swiss-Prot
Match:
I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)
HSP 1 Score: 620.9 bits (1600), Expect = 1.9e-176
Identity = 353/793 (44.51%), Postives = 478/793 (60.28%), Query Frame = 0
Query: 3 LSISILIFFSFLLLISPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRS 62
L + + FF F L++ +KK Y+V +G+HSHG T D E DSH+ LLGS S
Sbjct: 8 LHLILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGS 67
Query: 63 DEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLE 122
EKAK++I YSY +HINGFAA L++E+A +A +P V +V +K L+TT SWEF+ L
Sbjct: 68 REKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLH 127
Query: 123 KNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK---------- 182
+ G +S W+ +FG++ II N+DTGVWPES+SF + G G PSKW+
Sbjct: 128 RRG---QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGY-GTVPSKWRGGLCQINKLP 187
Query: 183 ------------GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYV 242
GA+Y+NK + E L ++++ RD+ GHG+HTLSTA GN+V
Sbjct: 188 GSMKNTCNRKLIGARYYNKAF------EAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFV 247
Query: 243 FGASVFGSGIGTAKGGSPKARVAAYKVCWPF-EQGGCFDADITEAFDHAIHDGVDVLSLS 302
GA VF G GTAKGGSP+ARVAAYKVCW + C+ AD+ A D AI DGVDV+++S
Sbjct: 248 PGARVFAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVS 307
Query: 303 LGGDPIKYSE----DSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTL 362
G + +E D I+I +FHA+ K I +V + GN GPTP T +N APW+ T+ ASTL
Sbjct: 308 FGVSYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTL 367
Query: 363 DREFYAPVVLQNGHRFMGSSHSKGLTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHS 422
DR+F + + + N G+S L + + LI AK NA DA LC+ TLD +
Sbjct: 368 DRDFSSNLTI-NNQLIEGASLFVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRT 427
Query: 423 KVKGKILVCLR-GETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLL-----PASH 482
KV GKI++C R G+ + +G +A AGA GMIL N +G ++ + H+ P
Sbjct: 428 KVNGKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRR 487
Query: 483 INYQDGQVLLSYINSARNPM--GYLIPPLAKVNT----KPAPTMAVFSSRGPNTISPEII 542
+ V + I +P+ G I +++ T KPAP MA FSSRGPN I P I+
Sbjct: 488 AKSRPHGVKTTAIGDEDDPLKTGDTI-KMSRARTLFGRKPAPVMASFSSRGPNKIQPSIL 547
Query: 543 KPDVTAPGVNIIAAFSEAISPTRDASDNRT-TPFITMSGTSMSCPHVAGLVGLLRNLHPD 602
KPDVTAPGVNI+AA+SE S + DNR F + GTSMSCPH +G+ GLL+ HP
Sbjct: 548 KPDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPS 607
Query: 603 WSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLS 662
WSP+AIKSAIMT++ DNT P+ D LA A FAYGSGH+ P AI+PGLVYDLS
Sbjct: 608 WSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADA--FAYGSGHVRPDLAIEPGLVYDLS 667
Query: 663 PNDYLEFLCASGYDEKTIRAFS-DEPFKCPPASSVLNLNYPSIGVQNLK-GSVSVTRKLK 722
DYL FLCASGYD++ I A + + F C + SV +LNYPSI + NL+ V++ R +
Sbjct: 668 LTDYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVT 727
Query: 723 NVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTL--AGVVPKDRVGYGALIW 752
NVG P Y PNG ++V P L F ++GE K+F++ + + + + +G L W
Sbjct: 728 NVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRW 786
BLAST of MELO3C026085 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 547.4 bits (1409), Expect = 2.6e-154
Identity = 327/779 (41.98%), Postives = 451/779 (57.89%), Query Frame = 0
Query: 3 LSISILIFFSFLLLISPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRS 62
+S S L +F LL+ S G++ + + + S D H S LRS
Sbjct: 1 MSSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYI--VHMAKSQMPSSFDLHSNWYDSSLRS 60
Query: 63 DEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLE 122
+ + + Y+Y+ I+GF+ L E+A L P V +VLP +L+TT + F+ L+
Sbjct: 61 ISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD 120
Query: 123 KN--GVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK-------- 182
++ + P + + DV++ LDTGVWPESKS+ + G GP PS WK
Sbjct: 121 EHTADLFPEAGSY------SDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTN 180
Query: 183 -----------GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVF 242
GA++F +GY +S +D S S RD DGHG+HT STAAG+ V
Sbjct: 181 FTASLCNRKLIGARFFARGY----ESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVE 240
Query: 243 GASVFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLG 302
GAS+ G GTA+G +P+ARVA YKVCW GGCF +DI A D AI D V+VLS+SLG
Sbjct: 241 GASLLGYASGTARGMAPRARVAVYKVCW---LGGCFSSDILAAIDKAIADNVNVLSMSLG 300
Query: 303 GDPIKYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYA 362
G Y D +AI +F A+++GI V C+ GN+GP+ + SN APWI TVGA TLDR+F A
Sbjct: 301 GGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPA 360
Query: 363 PVVLQNGHRFMGSSHSKG-LTGRKLYPLITGAQAKAGNA-NEDDAMLCKPETLDHSKVKG 422
+L NG F G S KG KL P I AGNA N + LC TL KVKG
Sbjct: 361 LAILGNGKNFTGVSLFKGEALPDKLLPFI-----YAGNASNATNGNLCMTGTLIPEKVKG 420
Query: 423 KILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLL 482
KI++C RG AR+ KG AG VGMIL N +G +V D HLLPA+ + + G ++
Sbjct: 421 KIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIR 480
Query: 483 SYINSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFS 542
Y+ + NP + V KP+P +A FSSRGPN+I+P I+KPD+ APGVNI+AA++
Sbjct: 481 HYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWT 540
Query: 543 EAISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVR 602
A PT ASD+R F +SGTSMSCPHV+GL LL+++HP+WSP+AI+SA+MT++
Sbjct: 541 GAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKT 600
Query: 603 DNTLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKT 662
P++D + P+TPF +G+GH++PT A +PGL+YDL+ DYL FLCA Y
Sbjct: 601 YKDGKPLLDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQ 660
Query: 663 IRAFSDEPFKCPPAS--SVLNLNYPSIGVQ-NLKGSVSVTRKLKNVGSPGVYRAQIL-HP 722
IR+ S + C P+ SV +LNYPS V + G+ TR + +VG G Y ++
Sbjct: 661 IRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSET 720
Query: 723 NGVVVSVKPRFLKFERVGEEKSFELTLA--GVVPKDRVGYGALIWSDGRHVVRSPIVVS 753
GV +SV+P L F+ E+KS+ +T P +G++ WSDG+HVV SP+ +S
Sbjct: 721 TGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755
BLAST of MELO3C026085 vs. ExPASy Swiss-Prot
Match:
O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)
HSP 1 Score: 533.9 bits (1374), Expect = 3.0e-150
Identity = 328/789 (41.57%), Postives = 458/789 (58.05%), Query Frame = 0
Query: 4 SISILIFFSFLLLISPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSD 63
+I +L+F SF + A A K+++ + S S+ +H+ S
Sbjct: 5 TIVLLLFLSFPFISFAASQAAKTFIFRIDGGS---------MPSIFPTHYHWY-----ST 64
Query: 64 EKAKDS-IFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLE 123
E A++S I + Y +GF+A + ++A L NHP V AV ++ ++L+TT S +F+ L+
Sbjct: 65 EFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ 124
Query: 124 KNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK---------- 183
W + +G DVII DTG+WPE +SF + + GP P +W+
Sbjct: 125 NQ-----KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGARFS 184
Query: 184 ---------GAKYFNKG-YLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFG 243
GA++F KG + N TV+ S RD DGHG+HT STAAG + F
Sbjct: 185 PRNCNRKIIGARFFAKGQQAAVIGGINKTVEFL----SPRDADGHGTHTSSTAAGRHAFK 244
Query: 244 ASVFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSL-G 303
AS+ G G AKG +PKAR+AAYKVCW + GC D+DI AFD A+ DGVDV+S+S+ G
Sbjct: 245 ASMSGYASGVAKGVAPKARIAAYKVCW--KDSGCLDSDILAAFDAAVRDGVDVISISIGG 304
Query: 304 GDPI--KYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREF 363
GD I Y D IAI S+ A KGI V + GN GP + +N APW+ TVGAST+DR F
Sbjct: 305 GDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNF 364
Query: 364 YAPVVLQNGHRFMGSSHSKG--LTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKV 423
A +L +GHR G S G L GR ++P++ G + A LC TLD +V
Sbjct: 365 PADAILGDGHRLRGVSLYAGVPLNGR-MFPVV-----YPGKSGMSSASLCMENTLDPKQV 424
Query: 424 KGKILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQV 483
+GKI++C RG + R+ KG AG VGMIL N +G +V D HL+PA + +G
Sbjct: 425 RGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDR 484
Query: 484 LLSYINSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAA 543
+ +Y +S NP+ + V KPAP +A FS RGPN +SPEI+KPD+ APGVNI+AA
Sbjct: 485 IKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAA 544
Query: 544 FSEAISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQ 603
+++A+ PT SD R T F +SGTSM+CPHV+G LL++ HPDWSP+ I+SA+MT++
Sbjct: 545 WTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTN 604
Query: 604 VRDNTLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDE 663
+ DN+ +ID + ATP+ YGSGH+N A++PGLVYD++ +DY+ FLC+ GY
Sbjct: 605 LVDNSNRSLIDEST--GKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGP 664
Query: 664 KTIRAFSDEPFKCPPA--SSVLNLNYPSIGV---QNLKGSVS--VTRKLKNVG-SPGVYR 723
KTI+ + P +CP S NLNYPSI N +G VS V R NVG + VYR
Sbjct: 665 KTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYR 724
Query: 724 AQILHPNGVVVSVKPRFLKFERVGEEKSFELTLA-----GVVPKDRVGYGALIWSD-GRH 753
A+I P GV V+VKP L F + +S+ +T+ V+ + +G++ W D G+H
Sbjct: 725 ARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKH 759
BLAST of MELO3C026085 vs. NCBI nr
Match:
TYK15479.1 (subtilisin-like protease SBT5.4 [Cucumis melo var. makuwa])
HSP 1 Score: 1471.8 bits (3809), Expect = 0.0e+00
Identity = 731/749 (97.60%), Postives = 731/749 (97.60%), Query Frame = 0
Query: 26 SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 85
SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL
Sbjct: 755 SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 814
Query: 86 DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIA 145
DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIA
Sbjct: 815 DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIA 874
Query: 146 NLDTGVWPESKSFGEHGIDGPAPSKWK------------------GAKYFNKGYLEYLKS 205
NLDTGVWPESKSFGEHGIDGPAPSKWK GAKYFNKGYLEYLKS
Sbjct: 875 NLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYLEYLKS 934
Query: 206 ENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYKV 265
ENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYKV
Sbjct: 935 ENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYKV 994
Query: 266 CWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPVV 325
CWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPVV
Sbjct: 995 CWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPVV 1054
Query: 326 CAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLYP 385
CAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLYP
Sbjct: 1055 CAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLYP 1114
Query: 386 LITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMI 445
LITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMI
Sbjct: 1115 LITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMI 1174
Query: 446 LCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTM 505
LCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTM
Sbjct: 1175 LCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTM 1234
Query: 506 AVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCP 565
AVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCP
Sbjct: 1235 AVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCP 1294
Query: 566 HVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHI 625
HVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHI
Sbjct: 1295 HVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHI 1354
Query: 626 NPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGVQ 685
NPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGVQ
Sbjct: 1355 NPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGVQ 1414
Query: 686 NLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVVP 745
NLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVVP
Sbjct: 1415 NLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVVP 1474
Query: 746 KDRVGYGALIWSDGRHVVRSPIVVSSGLF 757
KDRVGYGALIWSDGRHVVRSPIVVSSGLF
Sbjct: 1475 KDRVGYGALIWSDGRHVVRSPIVVSSGLF 1503
BLAST of MELO3C026085 vs. NCBI nr
Match:
XP_011648463.1 (subtilisin-like protease SBT5.4 [Cucumis sativus] >KGN64981.1 hypothetical protein Csa_022745 [Cucumis sativus])
HSP 1 Score: 1387.1 bits (3589), Expect = 0.0e+00
Identity = 690/775 (89.03%), Postives = 721/775 (93.03%), Query Frame = 0
Query: 1 MKLSISILIFFSFLLLISPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFL 60
MK IS LIFFSFLLLISPAIA KKSYVVLLGSHSHGL+ TEKDF+ VVDSHHKLLGSFL
Sbjct: 1 MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL 60
Query: 61 RSDEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMH 120
RS+EKAKD+IFYSYKK+INGFAATLDDEDATRLANHPEVAAVLPNK K+LYTTHSWEFMH
Sbjct: 61 RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH 120
Query: 121 LEKNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK-------- 180
LEKNGV+PPSSPW AKFGKDVIIANLDTGVWPESKSFGEHGI GPAPSKWK
Sbjct: 121 LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT 180
Query: 181 -----------GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVF 240
GAKYFNKGY EYLKSENSTVDLSSIINSTRDY+GHGSHTLSTA GNYV
Sbjct: 181 PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV 240
Query: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLG 300
GASVFGSGIGTAKGGSPKARVAAYKVCWP+E GGCFDADITEAFDHAIHDGVDVLSLSLG
Sbjct: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLG 300
Query: 301 GDPIKYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYA 360
D IKYSED+IAIASFHAVKKGIPVVCAVGNSGP PKTASNTAPWILTVGASTLDREFYA
Sbjct: 301 SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA 360
Query: 361 PVVLQNGHRFMGSSHSKGLTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKI 420
PVVL+NG++FMGSSHSKGL GR LYPLITGAQAKAGNA EDDAMLCKPETLDHSKVKGKI
Sbjct: 361 PVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKI 420
Query: 421 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSY 480
LVCLRGETARLDKGKQAALAGAVGMILCNDKLSG+SI PDFH+LPASHINY DGQVLLSY
Sbjct: 421 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSY 480
Query: 481 INSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEA 540
NSAR PMG LIPPLA+VNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGV+IIAAFSEA
Sbjct: 481 TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEA 540
Query: 541 ISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDN 600
ISPTRD SDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDW+PSAIKSAIMTS+QVRDN
Sbjct: 541 ISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDN 600
Query: 601 TLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIR 660
TLNPM+DGGSL L PATPFAYGSGHINPTGA+DPGLVYDLSPNDYLEFLCASGYDE+TIR
Sbjct: 601 TLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIR 660
Query: 661 AFSDEPFKCPPASSVLNLNYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVS 720
AFSDEPFKCP ++SVLNLNYPSIGVQNLK SV++TRKLKNVG+PGVY+AQILHPN V VS
Sbjct: 661 AFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVS 720
Query: 721 VKPRFLKFERVGEEKSFELTLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 757
VKPRFLKFERVGEEKSFELT++GVVPK+R YGALIWSDGRH VRSPIVVSSGLF
Sbjct: 721 VKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 775
BLAST of MELO3C026085 vs. NCBI nr
Match:
XP_038895602.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])
HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 622/774 (80.36%), Postives = 681/774 (87.98%), Query Frame = 0
Query: 1 MKLSISILIFFSFLLLISPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFL 60
MK+S SILI FSFLLLIS AIAAKKSYVVLLGSHSHG+E+TE+D E VV SHHKLLGSFL
Sbjct: 1 MKISNSILILFSFLLLISSAIAAKKSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFL 60
Query: 61 RSDEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMH 120
S+EKAKD+IFYSYKKHINGFAATLD+E+AT+LA HPEVAA+L N+ K L+TTHSWEFMH
Sbjct: 61 GSEEKAKDAIFYSYKKHINGFAATLDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMH 120
Query: 121 LEKNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK-------- 180
LEKNGV+P SSPWR+AK GKDVIIANLDTGVWPESKSFGEHGI GP PSKWK
Sbjct: 121 LEKNGVIPSSSPWRIAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETP 180
Query: 181 ----------GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFG 240
GAKYFNKG++ YLKSENST + SS+INSTRDY+GHGSHTLSTA G+YV G
Sbjct: 181 DRVPCNRKLIGAKYFNKGFIAYLKSENST-EFSSVINSTRDYEGHGSHTLSTAGGSYVSG 240
Query: 241 ASVFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGG 300
ASVFGSG GTAKGGSPKARVAAYKVCWP E GGCFD+DI EAFDHAIHDGVDVLSLSLG
Sbjct: 241 ASVFGSGAGTAKGGSPKARVAAYKVCWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGS 300
Query: 301 DPIKYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAP 360
DP +YSEDSIAIA+FHAVKKGIPVVC+ GNSGP TASNTAPWILTVGASTLDREF AP
Sbjct: 301 DPAEYSEDSIAIAAFHAVKKGIPVVCSAGNSGPDALTASNTAPWILTVGASTLDREFQAP 360
Query: 361 VVLQNGHRFMGSSHSKGLTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKIL 420
V LQNGHR+MGSS SK L G KLYPLITGAQAKA NA DDAMLCKPETLDHSKVKGKIL
Sbjct: 361 VELQNGHRYMGSSLSKALQGNKLYPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKIL 420
Query: 421 VCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYI 480
CLRG+ AR+DKG+QAALAGAVGMILCND+LSG + D H+LPASHINY DGQ +LSYI
Sbjct: 421 ACLRGDNARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVLSYI 480
Query: 481 NSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAI 540
NS +NPMGYLIPP AKVNTKPAPTMA FSSRGPN ISPEIIKPDVTAPGVN+IAAFSEA+
Sbjct: 481 NSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAV 540
Query: 541 SPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNT 600
SPT +A DNRT PFITMSGTSMSCPHV+GLVGLLR LHP WSPSAIKSAIMTS+++RDNT
Sbjct: 541 SPTGEAFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNT 600
Query: 601 LNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRA 660
+ PM+DGGS DLAPATPFAYGSGHI PTGAIDPGLVYDLSPNDYLEFLCASGYD+KTIRA
Sbjct: 601 MKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRA 660
Query: 661 FSDEPFKCPPASSVLNLNYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSV 720
FSD PFKCPP +S+LN NYPSIGVQNL G+V+VTRKLKNV +PGVYRA+I++P+GV VSV
Sbjct: 661 FSDGPFKCPPNASILNFNYPSIGVQNLNGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSV 720
Query: 721 KPRFLKFERVGEEKSFELTLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 757
KP+ LKFERV EEKSFELT+ G VP+D+V G LIW+DG+H VRSPIV+SS LF
Sbjct: 721 KPKVLKFERVAEEKSFELTMTGNVPEDQVVDGVLIWTDGKHFVRSPIVISSVLF 773
BLAST of MELO3C026085 vs. NCBI nr
Match:
XP_008464322.1 (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo])
HSP 1 Score: 1201.4 bits (3107), Expect = 0.0e+00
Identity = 595/774 (76.87%), Postives = 662/774 (85.53%), Query Frame = 0
Query: 1 MKLSISILIFFSFLLLISPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFL 60
MK+ SILI FS LL+ISPAIAAKKSY+VLLGSHSHGLEV ++D E V DSHHKLLGS +
Sbjct: 6 MKIYNSILILFSLLLVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKLLGSII 65
Query: 61 RSDEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMH 120
SDEKA+D+IFYSYK++INGFAA +D+E+AT+LA HPEVAAVL NK K L+TTHSWEFMH
Sbjct: 66 GSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHSWEFMH 125
Query: 121 LEKNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK-------- 180
LEKNGV+PPSS WR AK GKDVII NLDTGVW ESKSFGEHGI G PSKWK
Sbjct: 126 LEKNGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGCTDKTP 185
Query: 181 ----------GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFG 240
GAKYFNKG+L YL S+N T +S+INSTRDYDGHGSHTLSTA G+YV G
Sbjct: 186 DGVSCNRKLIGAKYFNKGFLAYLNSQNLT---ASVINSTRDYDGHGSHTLSTAGGSYVSG 245
Query: 241 ASVFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGG 300
ASVFG G+GTAKGGSPKARVA+YKVCWP E GGCF+ADI EAFDHAIHD VDVLSLSLGG
Sbjct: 246 ASVFGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSLGG 305
Query: 301 DPIKYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAP 360
+P Y +D IAIA+FHAVKKGIPVVC+ GNSGP +T SNTAPWILTVGASTLDREF AP
Sbjct: 306 EPADYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDREFQAP 365
Query: 361 VVLQNGHRFMGSSHSKGLTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKIL 420
V LQNGH +MGSS SKGL G KLYPLITGA+AKA NA + AMLCKP+TLDHSKVKGKIL
Sbjct: 366 VELQNGHSYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKIL 425
Query: 421 VCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYI 480
VCLRG+TAR+DKG+QAALAGAVGMILCNDKLSG + D H+LPASHINY DGQ + SYI
Sbjct: 426 VCLRGDTARVDKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASHINYNDGQAVFSYI 485
Query: 481 NSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAI 540
S +NPMG LIPP AKVNTKPAP+MA FSSRGPN ISPEIIKPDVTAPGVNIIAAFSEA+
Sbjct: 486 KSTKNPMGSLIPPSAKVNTKPAPSMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAV 545
Query: 541 SPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNT 600
SPT + DNRT PFITMSGTSMSCPHV+GLVGLLR LHP WSPSAIKSAIMTS+++RDNT
Sbjct: 546 SPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNT 605
Query: 601 LNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRA 660
PM+DGGS DLAPATPFAYGSGHI PTGAIDPGLVYDLSPNDYLEFLCASGY+EKTI+A
Sbjct: 606 KKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQA 665
Query: 661 FSDEPFKCPPASSVLNLNYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSV 720
FSD PFKCP ++S+LN NYPSIGVQNL GSV++TRKLKNV +PGVY+A+++HPNGV V V
Sbjct: 666 FSDGPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLKNVSTPGVYKARVMHPNGVKVLV 725
Query: 721 KPRFLKFERVGEEKSFELTLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 757
KP+ LKFERVGEEK FEL + G VP+++V G LIW+DG+H VRSPIVVSSGLF
Sbjct: 726 KPKVLKFERVGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776
BLAST of MELO3C026085 vs. NCBI nr
Match:
KAE8652826.1 (hypothetical protein Csa_022873 [Cucumis sativus])
HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 584/756 (77.25%), Postives = 651/756 (86.11%), Query Frame = 0
Query: 21 IAAKK--SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHI 80
+AAKK SY+VLLGSHSHGLEVT++D + V DSHHKLLGS SDEKA+++IFYSYKK+I
Sbjct: 1 MAAKKNISYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNI 60
Query: 81 NGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKF 140
NGFAA +D+E+A +LA HPEVAAVLPN+ K L+TTHSWEFMHLEKNGV+PPSS WR AK
Sbjct: 61 NGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKS 120
Query: 141 GKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK------------------GAKYFNKG 200
GKDVIIANLDTGVWPESKSFGEHGI GP PSKWK GAKYFNKG
Sbjct: 121 GKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKG 180
Query: 201 YLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKA 260
+L YLKSEN T + +INSTRDYDGHGSHTLSTA G+YV GASVFG G+GTAKGGSPKA
Sbjct: 181 FLAYLKSENLT---ALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKA 240
Query: 261 RVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAV 320
RVAAYKVCWP E GGCFDADI +AFDHAIHD VDVLSLSLGG+P Y +D IAI++FHAV
Sbjct: 241 RVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAV 300
Query: 321 KKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGL 380
KKGIPVVC+ GNSGP +T SNTAPWILTVGAST+DREF APV LQNGHR+MGSS SKGL
Sbjct: 301 KKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGL 360
Query: 381 TGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAAL 440
G KLYPLITGA+AKA NA ++A LCKP+TLDHSKVKGKILVCLRG+TAR+DKG+QAAL
Sbjct: 361 KGDKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAAL 420
Query: 441 AGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVN 500
AGAVGMILCND+LSG + D H+LPASHINY DGQ + SYI + +NPMGYLIPP AKVN
Sbjct: 421 AGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVN 480
Query: 501 TKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMS 560
TKPAPTMA FSSRGPN ISPEIIKPDVTAPGVNIIAAFSEA+SPT + DNRT PFITMS
Sbjct: 481 TKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMS 540
Query: 561 GTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPF 620
GTSMSCPHV+GLVGLLR LHP WSPSAIKSAIMTS+++RDNT PM+DGGS DLAP+TPF
Sbjct: 541 GTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPF 600
Query: 621 AYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLN 680
AYGSGHI PTGAIDPGLVYDLSPNDYLEFLCASGY+EKTI+AFSD PFKCP ++S+LNLN
Sbjct: 601 AYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLN 660
Query: 681 YPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFEL 740
YPSIGVQNL GSV+VTRKLKNV +PGVY+ ++ HPNGV V VKP+ LKFERVGEEKSFEL
Sbjct: 661 YPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFEL 720
Query: 741 TLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 757
T+ G VP+D+V G LIW+DG+H VRSPIVVSS LF
Sbjct: 721 TITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 753
BLAST of MELO3C026085 vs. ExPASy TrEMBL
Match:
A0A5D3CZ66 (Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00570 PE=3 SV=1)
HSP 1 Score: 1471.8 bits (3809), Expect = 0.0e+00
Identity = 731/749 (97.60%), Postives = 731/749 (97.60%), Query Frame = 0
Query: 26 SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 85
SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL
Sbjct: 755 SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 814
Query: 86 DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIA 145
DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIA
Sbjct: 815 DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIA 874
Query: 146 NLDTGVWPESKSFGEHGIDGPAPSKWK------------------GAKYFNKGYLEYLKS 205
NLDTGVWPESKSFGEHGIDGPAPSKWK GAKYFNKGYLEYLKS
Sbjct: 875 NLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYLEYLKS 934
Query: 206 ENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYKV 265
ENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYKV
Sbjct: 935 ENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYKV 994
Query: 266 CWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPVV 325
CWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPVV
Sbjct: 995 CWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPVV 1054
Query: 326 CAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLYP 385
CAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLYP
Sbjct: 1055 CAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLYP 1114
Query: 386 LITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMI 445
LITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMI
Sbjct: 1115 LITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMI 1174
Query: 446 LCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTM 505
LCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTM
Sbjct: 1175 LCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTM 1234
Query: 506 AVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCP 565
AVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCP
Sbjct: 1235 AVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCP 1294
Query: 566 HVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHI 625
HVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHI
Sbjct: 1295 HVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHI 1354
Query: 626 NPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGVQ 685
NPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGVQ
Sbjct: 1355 NPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGVQ 1414
Query: 686 NLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVVP 745
NLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVVP
Sbjct: 1415 NLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVVP 1474
Query: 746 KDRVGYGALIWSDGRHVVRSPIVVSSGLF 757
KDRVGYGALIWSDGRHVVRSPIVVSSGLF
Sbjct: 1475 KDRVGYGALIWSDGRHVVRSPIVVSSGLF 1503
BLAST of MELO3C026085 vs. ExPASy TrEMBL
Match:
A0A0A0LYF1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=3 SV=1)
HSP 1 Score: 1387.1 bits (3589), Expect = 0.0e+00
Identity = 690/775 (89.03%), Postives = 721/775 (93.03%), Query Frame = 0
Query: 1 MKLSISILIFFSFLLLISPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFL 60
MK IS LIFFSFLLLISPAIA KKSYVVLLGSHSHGL+ TEKDF+ VVDSHHKLLGSFL
Sbjct: 1 MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL 60
Query: 61 RSDEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMH 120
RS+EKAKD+IFYSYKK+INGFAATLDDEDATRLANHPEVAAVLPNK K+LYTTHSWEFMH
Sbjct: 61 RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH 120
Query: 121 LEKNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK-------- 180
LEKNGV+PPSSPW AKFGKDVIIANLDTGVWPESKSFGEHGI GPAPSKWK
Sbjct: 121 LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT 180
Query: 181 -----------GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVF 240
GAKYFNKGY EYLKSENSTVDLSSIINSTRDY+GHGSHTLSTA GNYV
Sbjct: 181 PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV 240
Query: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLG 300
GASVFGSGIGTAKGGSPKARVAAYKVCWP+E GGCFDADITEAFDHAIHDGVDVLSLSLG
Sbjct: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLG 300
Query: 301 GDPIKYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYA 360
D IKYSED+IAIASFHAVKKGIPVVCAVGNSGP PKTASNTAPWILTVGASTLDREFYA
Sbjct: 301 SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA 360
Query: 361 PVVLQNGHRFMGSSHSKGLTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKI 420
PVVL+NG++FMGSSHSKGL GR LYPLITGAQAKAGNA EDDAMLCKPETLDHSKVKGKI
Sbjct: 361 PVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKI 420
Query: 421 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSY 480
LVCLRGETARLDKGKQAALAGAVGMILCNDKLSG+SI PDFH+LPASHINY DGQVLLSY
Sbjct: 421 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSY 480
Query: 481 INSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEA 540
NSAR PMG LIPPLA+VNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGV+IIAAFSEA
Sbjct: 481 TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEA 540
Query: 541 ISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDN 600
ISPTRD SDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDW+PSAIKSAIMTS+QVRDN
Sbjct: 541 ISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDN 600
Query: 601 TLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIR 660
TLNPM+DGGSL L PATPFAYGSGHINPTGA+DPGLVYDLSPNDYLEFLCASGYDE+TIR
Sbjct: 601 TLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIR 660
Query: 661 AFSDEPFKCPPASSVLNLNYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVS 720
AFSDEPFKCP ++SVLNLNYPSIGVQNLK SV++TRKLKNVG+PGVY+AQILHPN V VS
Sbjct: 661 AFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVS 720
Query: 721 VKPRFLKFERVGEEKSFELTLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 757
VKPRFLKFERVGEEKSFELT++GVVPK+R YGALIWSDGRH VRSPIVVSSGLF
Sbjct: 721 VKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 775
BLAST of MELO3C026085 vs. ExPASy TrEMBL
Match:
A0A1S3CLB7 (subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=1)
HSP 1 Score: 1201.4 bits (3107), Expect = 0.0e+00
Identity = 595/774 (76.87%), Postives = 662/774 (85.53%), Query Frame = 0
Query: 1 MKLSISILIFFSFLLLISPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFL 60
MK+ SILI FS LL+ISPAIAAKKSY+VLLGSHSHGLEV ++D E V DSHHKLLGS +
Sbjct: 6 MKIYNSILILFSLLLVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKLLGSII 65
Query: 61 RSDEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMH 120
SDEKA+D+IFYSYK++INGFAA +D+E+AT+LA HPEVAAVL NK K L+TTHSWEFMH
Sbjct: 66 GSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHSWEFMH 125
Query: 121 LEKNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK-------- 180
LEKNGV+PPSS WR AK GKDVII NLDTGVW ESKSFGEHGI G PSKWK
Sbjct: 126 LEKNGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGCTDKTP 185
Query: 181 ----------GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFG 240
GAKYFNKG+L YL S+N T +S+INSTRDYDGHGSHTLSTA G+YV G
Sbjct: 186 DGVSCNRKLIGAKYFNKGFLAYLNSQNLT---ASVINSTRDYDGHGSHTLSTAGGSYVSG 245
Query: 241 ASVFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGG 300
ASVFG G+GTAKGGSPKARVA+YKVCWP E GGCF+ADI EAFDHAIHD VDVLSLSLGG
Sbjct: 246 ASVFGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSLGG 305
Query: 301 DPIKYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAP 360
+P Y +D IAIA+FHAVKKGIPVVC+ GNSGP +T SNTAPWILTVGASTLDREF AP
Sbjct: 306 EPADYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDREFQAP 365
Query: 361 VVLQNGHRFMGSSHSKGLTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKIL 420
V LQNGH +MGSS SKGL G KLYPLITGA+AKA NA + AMLCKP+TLDHSKVKGKIL
Sbjct: 366 VELQNGHSYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKIL 425
Query: 421 VCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYI 480
VCLRG+TAR+DKG+QAALAGAVGMILCNDKLSG + D H+LPASHINY DGQ + SYI
Sbjct: 426 VCLRGDTARVDKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASHINYNDGQAVFSYI 485
Query: 481 NSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAI 540
S +NPMG LIPP AKVNTKPAP+MA FSSRGPN ISPEIIKPDVTAPGVNIIAAFSEA+
Sbjct: 486 KSTKNPMGSLIPPSAKVNTKPAPSMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAV 545
Query: 541 SPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNT 600
SPT + DNRT PFITMSGTSMSCPHV+GLVGLLR LHP WSPSAIKSAIMTS+++RDNT
Sbjct: 546 SPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNT 605
Query: 601 LNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRA 660
PM+DGGS DLAPATPFAYGSGHI PTGAIDPGLVYDLSPNDYLEFLCASGY+EKTI+A
Sbjct: 606 KKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQA 665
Query: 661 FSDEPFKCPPASSVLNLNYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSV 720
FSD PFKCP ++S+LN NYPSIGVQNL GSV++TRKLKNV +PGVY+A+++HPNGV V V
Sbjct: 666 FSDGPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLKNVSTPGVYKARVMHPNGVKVLV 725
Query: 721 KPRFLKFERVGEEKSFELTLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 757
KP+ LKFERVGEEK FEL + G VP+++V G LIW+DG+H VRSPIVVSSGLF
Sbjct: 726 KPKVLKFERVGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776
BLAST of MELO3C026085 vs. ExPASy TrEMBL
Match:
A0A0A0LVY8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1)
HSP 1 Score: 1189.5 bits (3076), Expect = 0.0e+00
Identity = 580/750 (77.33%), Postives = 647/750 (86.27%), Query Frame = 0
Query: 25 KSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAAT 84
+SY+VLLGSHSHGLEVT++D + V DSHHKLLGS SDEKA+++IFYSYKK+INGFAA
Sbjct: 11 QSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNINGFAAI 70
Query: 85 LDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVII 144
+D+E+A +LA HPEVAAVLPN+ K L+TTHSWEFMHLEKNGV+PPSS WR AK GKDVII
Sbjct: 71 MDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVII 130
Query: 145 ANLDTGVWPESKSFGEHGIDGPAPSKWK------------------GAKYFNKGYLEYLK 204
ANLDTGVWPESKSFGEHGI GP PSKWK GAKYFNKG+L YLK
Sbjct: 131 ANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFLAYLK 190
Query: 205 SENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYK 264
SEN T + +INSTRDYDGHGSHTLSTA G+YV GASVFG G+GTAKGGSPKARVAAYK
Sbjct: 191 SENLT---ALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAYK 250
Query: 265 VCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPV 324
VCWP E GGCFDADI +AFDHAIHD VDVLSLSLGG+P Y +D IAI++FHAVKKGIPV
Sbjct: 251 VCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIPV 310
Query: 325 VCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLY 384
VC+ GNSGP +T SNTAPWILTVGAST+DREF APV LQNGHR+MGSS SKGL G KLY
Sbjct: 311 VCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKLY 370
Query: 385 PLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGM 444
PLITGA+AKA NA ++A LCKP+TLDHSKVKGKILVCLRG+TAR+DKG+QAALAGAVGM
Sbjct: 371 PLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGM 430
Query: 445 ILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPT 504
ILCND+LSG + D H+LPASHINY DGQ + SYI + +NPMGYLIPP AKVNTKPAPT
Sbjct: 431 ILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNTKPAPT 490
Query: 505 MAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSC 564
MA FSSRGPN ISPEIIKPDVTAPGVNIIAAFSEA+SPT + DNRT PFITMSGTSMSC
Sbjct: 491 MAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSC 550
Query: 565 PHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGH 624
PHV+GLVGLLR LHP WSPSAIKSAIMTS+++RDNT PM+DGGS DLAP+TPFAYGSGH
Sbjct: 551 PHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGH 610
Query: 625 INPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGV 684
I PTGAIDPGLVYDLSPNDYLEFLCASGY+EKTI+AFSD PFKCP ++S+LNLNYPSIGV
Sbjct: 611 IRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGV 670
Query: 685 QNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVV 744
QNL GSV+VTRKLKNV +PGVY+ ++ HPNGV V VKP+ LKFERVGEEKSFELT+ G V
Sbjct: 671 QNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGDV 730
Query: 745 PKDRVGYGALIWSDGRHVVRSPIVVSSGLF 757
P+D+V G LIW+DG+H VRSPIVVSS LF
Sbjct: 731 PEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757
BLAST of MELO3C026085 vs. ExPASy TrEMBL
Match:
A0A6J1CN43 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 PE=3 SV=1)
HSP 1 Score: 1174.1 bits (3036), Expect = 0.0e+00
Identity = 580/779 (74.45%), Postives = 658/779 (84.47%), Query Frame = 0
Query: 1 MKLSISILIFFS----FLLLISPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLL 60
MKLS SIL FS F LLISPAIAAKKSYVV+LGSHSHGLEV+E D + VVDSHHKLL
Sbjct: 1 MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL 60
Query: 61 GSFLRSDEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSW 120
GSFL S EKAKD+IFYSYKK+INGFAA L++E+A LA HPEVAAVL NK K L+TTHSW
Sbjct: 61 GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW 120
Query: 121 EFMHLEK-NGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK--- 180
FM LEK NGVVPP+SPW A FG+D IIANLDTGVWPESKSFGE+GI G P+KWK
Sbjct: 121 NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC 180
Query: 181 ---------------GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAG 240
GAKYFNKG++ YLK+ NS+ DLSSI NSTRDY+GHGSHTLSTA G
Sbjct: 181 TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 240
Query: 241 NYVFGASVFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLS 300
++V ASVFGSG+GTAKGGSPKARVAAYK+CWPF+ GGCFDADITE FDHAIHDGV+V+S
Sbjct: 241 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 300
Query: 301 LSLGGDPIKYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDR 360
LS+GG P Y +DSIAIA+FHAVKKGIPVVC+ GNSGP TASNTAPWILTVGASTLDR
Sbjct: 301 LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR 360
Query: 361 EFYAPVVLQNGHRFMGSSHSKGLTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKV 420
+F APV L+NG RF GSS S L KLYPLITGAQAKA NA+ DAMLCKPETLDHSK
Sbjct: 361 QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA 420
Query: 421 KGKILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQV 480
KGKIL CLRG+ AR+DKG+QAALAGA GMILCND+LSG + D HLLPASH+NY DGQ
Sbjct: 421 KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA 480
Query: 481 LLSYINSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAA 540
+L YI S +NPMGYLIPP AK+NTKPAP MA FSSRGPN ++PEIIKPDVTAPGVN+IAA
Sbjct: 481 VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA 540
Query: 541 FSEAISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQ 600
++EA+SPT + DNRT PFITMSGTSMSCPHVAGL GLLR+LHP+WSPSAIKSAIMTS++
Sbjct: 541 YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR 600
Query: 601 VRDNTLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDE 660
+RDNT+ PM+DGG+ +LAPATPF+YGSGHI P GA+DPGLVYDL+P+DYLEFLCA GYDE
Sbjct: 601 IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE 660
Query: 661 KTIRAFSDEPFKCPPASSVLNLNYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNG 720
K IRAFSD P+KCPP++S+LN NYPSIGVQN+ GSV+VTR+LKNVG+PGVYRA++ P G
Sbjct: 661 KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG 720
Query: 721 VVVSVKPRFLKFERVGEEKSFELTLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 757
V VSV+PRFLKF++VGEEKSF+LT+AGVVP RV G LIW+DG H VRSPIV+SSGLF
Sbjct: 721 VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779
BLAST of MELO3C026085 vs. TAIR 10
Match:
AT5G59810.1 (Subtilase family protein )
HSP 1 Score: 852.8 bits (2202), Expect = 2.1e-247
Identity = 440/769 (57.22%), Postives = 542/769 (70.48%), Query Frame = 0
Query: 5 ISILIFFSFLLLI----SPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFL 64
+S+ S LLL+ SPA A KKSY+V LGSH+H +++ + V SH L SF+
Sbjct: 16 MSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFV 75
Query: 65 RSDEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMH 124
S E AK++IFYSYK+HINGFAA LD+ +A +A HP+V +V PNK + L+TTHSW FM
Sbjct: 76 GSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFML 135
Query: 125 LEKNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK-------- 184
L KNGVV SS W A +G+D IIANLDTGVWPESKSF + G G P++WK
Sbjct: 136 LAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGY-GAVPARWKGRCHKDVP 195
Query: 185 ------GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVF 244
GA+YFNKGYL Y + + ++ + RD+DGHGSHTLSTAAGN+V GA+VF
Sbjct: 196 CNRKLIGARYFNKGYLAY-----TGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVF 255
Query: 245 GSGIGTAKGGSPKARVAAYKVCWPFEQGG-CFDADITEAFDHAIHDGVDVLSLSLGGDPI 304
G G GTA GGSPKARVAAYKVCWP G CFDADI A + AI DGVDVLS S+GGD
Sbjct: 256 GIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAG 315
Query: 305 KYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVL 364
Y D IAI SFHAVK G+ VVC+ GNSGP T SN APW++TVGAS++DREF A V L
Sbjct: 316 DYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVEL 375
Query: 365 QNGHRFMGSSHSKGLTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCL 424
+NG F G+S SK L K+Y LI+ A A N N DA+LCK +LD KVKGKILVCL
Sbjct: 376 KNGQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCL 435
Query: 425 RGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSA 484
RG+ AR+DKG QAA AGA GM+LCNDK SG+ I+ D H+LPAS I+Y+DG+ L SY++S
Sbjct: 436 RGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSST 495
Query: 485 RNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPT 544
++P GY+ P A +NTKPAP MA FSSRGPNTI+P I+KPD+TAPGVNIIAAF+EA PT
Sbjct: 496 KDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPT 555
Query: 545 RDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNP 604
SDNR TPF T SGTSMSCPH++G+VGLL+ LHP WSP+AI+SAIMT+S+ R+N P
Sbjct: 556 DLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKP 615
Query: 605 MIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSD 664
M+D A PF+YGSGH+ P A PGLVYDL+ DYL+FLCA GY+ ++ F++
Sbjct: 616 MVDE---SFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAE 675
Query: 665 EP-FKCPPASSVLNLNYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKP 724
+P + C +++L+ NYPSI V NL GS++VTRKLKNVG P Y A+ P GV VSV+P
Sbjct: 676 DPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEP 735
Query: 725 RFLKFERVGEEKSFELTL--AGVVPKDRVGYGALIWSDGRHVVRSPIVV 752
+ L F + GE K F++TL V P V +G L W+D H VRSPIVV
Sbjct: 736 KQLTFNKTGEVKIFQMTLRPLPVTPSGYV-FGELTWTDSHHYVRSPIVV 774
BLAST of MELO3C026085 vs. TAIR 10
Match:
AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 761.5 bits (1965), Expect = 6.3e-220
Identity = 400/775 (51.61%), Postives = 523/775 (67.48%), Query Frame = 0
Query: 11 FSFLLLI------SPAIAAKK---SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLR 70
FSFLLL+ S I A K SYVV G+HSH E+TE + V ++H+ LGSF
Sbjct: 7 FSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTG 66
Query: 71 SDEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHL 130
S E+A D+IFYSY KHINGFAA LD + A ++ HPEV +V PNK L+TT SW+F+ L
Sbjct: 67 SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 126
Query: 131 EKNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK--------- 190
E N VP SS WR A+FG+D IIANLDTGVWPESKSF + G+ GP PS+WK
Sbjct: 127 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDA 186
Query: 191 ---------GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGA 250
GA+YFNKGY + NS+ D S RD DGHGSHTLSTAAG++V G
Sbjct: 187 TFHCNRKLIGARYFNKGYAAAVGHLNSSFD------SPRDLDGHGSHTLSTAAGDFVPGV 246
Query: 251 SVFGSGIGTAKGGSPKARVAAYKVCWPFEQGG-CFDADITEAFDHAIHDGVDVLSLSLGG 310
S+FG G GTAKGGSP+ARVAAYKVCWP +G C+DAD+ AFD AIHDG DV+S+SLGG
Sbjct: 247 SIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGG 306
Query: 311 DPIKYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAP 370
+P + DS+AI SFHA KK I VVC+ GNSGP T SN APW +TVGAST+DREF +
Sbjct: 307 EPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASN 366
Query: 371 VVLQNGHRFMGSS-HSKGLTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKI 430
+VL NG + G S S L K YP++ AKA NA+ DA LCK +LD K KGKI
Sbjct: 367 LVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKI 426
Query: 431 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSY 490
LVCLRG+ R++KG+ AL G +GM+L N ++G+ ++ D H+LPA+ + +D + Y
Sbjct: 427 LVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRY 486
Query: 491 INSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEA 550
I+ + P+ ++ P + KPAP MA FSS+GP+ ++P+I+KPD+TAPGV++IAA++ A
Sbjct: 487 ISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGA 546
Query: 551 ISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDN 610
+SPT + D R F +SGTSMSCPH++G+ GLL+ +P WSP+AI+SAIMT++ + D+
Sbjct: 547 VSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDD 606
Query: 611 TLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIR 670
P+ + ++ ATPF++G+GH+ P A++PGLVYDL DYL FLC+ GY+ I
Sbjct: 607 IPGPIQNATNM---KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQIS 666
Query: 671 AFSDEPFKC-PPASSVLNLNYPSIGVQNLKGS-VSVTRKLKNVGSPGVYRAQILHPNGVV 730
FS F C P S++NLNYPSI V NL S V+V+R +KNVG P +Y ++ +P GV
Sbjct: 667 VFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVY 726
Query: 731 VSVKPRFLKFERVGEEKSFELTLA---GVVPKDRVGYGALIWSDGRHVVRSPIVV 752
V+VKP L F +VGE+K+F++ L G V K V +G L+WSD +H VRSPIVV
Sbjct: 727 VAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYV-FGELVWSDKKHRVRSPIVV 770
BLAST of MELO3C026085 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 547.4 bits (1409), Expect = 1.9e-155
Identity = 327/779 (41.98%), Postives = 451/779 (57.89%), Query Frame = 0
Query: 3 LSISILIFFSFLLLISPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRS 62
+S S L +F LL+ S G++ + + + S D H S LRS
Sbjct: 1 MSSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYI--VHMAKSQMPSSFDLHSNWYDSSLRS 60
Query: 63 DEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLE 122
+ + + Y+Y+ I+GF+ L E+A L P V +VLP +L+TT + F+ L+
Sbjct: 61 ISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD 120
Query: 123 KN--GVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK-------- 182
++ + P + + DV++ LDTGVWPESKS+ + G GP PS WK
Sbjct: 121 EHTADLFPEAGSY------SDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTN 180
Query: 183 -----------GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVF 242
GA++F +GY +S +D S S RD DGHG+HT STAAG+ V
Sbjct: 181 FTASLCNRKLIGARFFARGY----ESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVE 240
Query: 243 GASVFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLG 302
GAS+ G GTA+G +P+ARVA YKVCW GGCF +DI A D AI D V+VLS+SLG
Sbjct: 241 GASLLGYASGTARGMAPRARVAVYKVCW---LGGCFSSDILAAIDKAIADNVNVLSMSLG 300
Query: 303 GDPIKYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYA 362
G Y D +AI +F A+++GI V C+ GN+GP+ + SN APWI TVGA TLDR+F A
Sbjct: 301 GGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPA 360
Query: 363 PVVLQNGHRFMGSSHSKG-LTGRKLYPLITGAQAKAGNA-NEDDAMLCKPETLDHSKVKG 422
+L NG F G S KG KL P I AGNA N + LC TL KVKG
Sbjct: 361 LAILGNGKNFTGVSLFKGEALPDKLLPFI-----YAGNASNATNGNLCMTGTLIPEKVKG 420
Query: 423 KILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLL 482
KI++C RG AR+ KG AG VGMIL N +G +V D HLLPA+ + + G ++
Sbjct: 421 KIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIR 480
Query: 483 SYINSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFS 542
Y+ + NP + V KP+P +A FSSRGPN+I+P I+KPD+ APGVNI+AA++
Sbjct: 481 HYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWT 540
Query: 543 EAISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVR 602
A PT ASD+R F +SGTSMSCPHV+GL LL+++HP+WSP+AI+SA+MT++
Sbjct: 541 GAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKT 600
Query: 603 DNTLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKT 662
P++D + P+TPF +G+GH++PT A +PGL+YDL+ DYL FLCA Y
Sbjct: 601 YKDGKPLLDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQ 660
Query: 663 IRAFSDEPFKCPPAS--SVLNLNYPSIGVQ-NLKGSVSVTRKLKNVGSPGVYRAQIL-HP 722
IR+ S + C P+ SV +LNYPS V + G+ TR + +VG G Y ++
Sbjct: 661 IRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSET 720
Query: 723 NGVVVSVKPRFLKFERVGEEKSFELTLA--GVVPKDRVGYGALIWSDGRHVVRSPIVVS 753
GV +SV+P L F+ E+KS+ +T P +G++ WSDG+HVV SP+ +S
Sbjct: 721 TGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755
BLAST of MELO3C026085 vs. TAIR 10
Match:
AT4G34980.1 (subtilisin-like serine protease 2 )
HSP 1 Score: 533.9 bits (1374), Expect = 2.1e-151
Identity = 328/789 (41.57%), Postives = 458/789 (58.05%), Query Frame = 0
Query: 4 SISILIFFSFLLLISPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSD 63
+I +L+F SF + A A K+++ + S S+ +H+ S
Sbjct: 5 TIVLLLFLSFPFISFAASQAAKTFIFRIDGGS---------MPSIFPTHYHWY-----ST 64
Query: 64 EKAKDS-IFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLE 123
E A++S I + Y +GF+A + ++A L NHP V AV ++ ++L+TT S +F+ L+
Sbjct: 65 EFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ 124
Query: 124 KNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK---------- 183
W + +G DVII DTG+WPE +SF + + GP P +W+
Sbjct: 125 NQ-----KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGARFS 184
Query: 184 ---------GAKYFNKG-YLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFG 243
GA++F KG + N TV+ S RD DGHG+HT STAAG + F
Sbjct: 185 PRNCNRKIIGARFFAKGQQAAVIGGINKTVEFL----SPRDADGHGTHTSSTAAGRHAFK 244
Query: 244 ASVFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSL-G 303
AS+ G G AKG +PKAR+AAYKVCW + GC D+DI AFD A+ DGVDV+S+S+ G
Sbjct: 245 ASMSGYASGVAKGVAPKARIAAYKVCW--KDSGCLDSDILAAFDAAVRDGVDVISISIGG 304
Query: 304 GDPI--KYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREF 363
GD I Y D IAI S+ A KGI V + GN GP + +N APW+ TVGAST+DR F
Sbjct: 305 GDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNF 364
Query: 364 YAPVVLQNGHRFMGSSHSKG--LTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKV 423
A +L +GHR G S G L GR ++P++ G + A LC TLD +V
Sbjct: 365 PADAILGDGHRLRGVSLYAGVPLNGR-MFPVV-----YPGKSGMSSASLCMENTLDPKQV 424
Query: 424 KGKILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQV 483
+GKI++C RG + R+ KG AG VGMIL N +G +V D HL+PA + +G
Sbjct: 425 RGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDR 484
Query: 484 LLSYINSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAA 543
+ +Y +S NP+ + V KPAP +A FS RGPN +SPEI+KPD+ APGVNI+AA
Sbjct: 485 IKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAA 544
Query: 544 FSEAISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQ 603
+++A+ PT SD R T F +SGTSM+CPHV+G LL++ HPDWSP+ I+SA+MT++
Sbjct: 545 WTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTN 604
Query: 604 VRDNTLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDE 663
+ DN+ +ID + ATP+ YGSGH+N A++PGLVYD++ +DY+ FLC+ GY
Sbjct: 605 LVDNSNRSLIDEST--GKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGP 664
Query: 664 KTIRAFSDEPFKCPPA--SSVLNLNYPSIGV---QNLKGSVS--VTRKLKNVG-SPGVYR 723
KTI+ + P +CP S NLNYPSI N +G VS V R NVG + VYR
Sbjct: 665 KTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYR 724
Query: 724 AQILHPNGVVVSVKPRFLKFERVGEEKSFELTLA-----GVVPKDRVGYGALIWSD-GRH 753
A+I P GV V+VKP L F + +S+ +T+ V+ + +G++ W D G+H
Sbjct: 725 ARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKH 759
BLAST of MELO3C026085 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 531.2 bits (1367), Expect = 1.4e-150
Identity = 318/787 (40.41%), Postives = 448/787 (56.93%), Query Frame = 0
Query: 10 FFSFLLLISPAIAAKKSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDS 69
FF L L SP+ +A S S ++ + V + S+ +H S L S + S
Sbjct: 7 FFFLLTLSSPSSSASSS-----NSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPS 66
Query: 70 IFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHL---EKNGV 129
I ++Y +GF+A L +DA++L +HP V +V+P + + L+TT S EF+ L +K G+
Sbjct: 67 IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGL 126
Query: 130 VPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWK-------------- 189
+ S FG D++I +DTGVWPE SF + G+ GP P KWK
Sbjct: 127 LEESD------FGSDLVIGVIDTGVWPERPSFDDRGL-GPVPIKWKGQCIASQDFPESAC 186
Query: 190 -----GAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFG 249
GA++F GY ++ N ++ ++ S RD DGHG+HT S +AG YVF AS G
Sbjct: 187 NRKLVGARFFCGGY----EATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLG 246
Query: 250 SGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKY 309
G A G +PKAR+AAYKVCW GC+D+DI AFD A+ DGVDV+SLS+GG + Y
Sbjct: 247 YAHGVAAGMAPKARLAAYKVCW---NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPY 306
Query: 310 SEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQN 369
D+IAI +F A+ +GI V + GN GP T +N APW+ TVGA T+DR+F A V L N
Sbjct: 307 YLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGN 366
Query: 370 GHRFMGSS--HSKGLTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCL 429
G G S GL ++YPL+ G G+ + LC +LD + VKGKI++C
Sbjct: 367 GKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGY--SSSLCLEGSLDPNLVKGKIVLCD 426
Query: 430 RGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYIN-- 489
RG +R KG+ G +GMI+ N G +V D H+LPA+ + G + YI+
Sbjct: 427 RGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISES 486
Query: 490 ----SARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFS 549
S+++P ++ ++ +PAP +A FS+RGPN +PEI+KPDV APG+NI+AA+
Sbjct: 487 SKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWP 546
Query: 550 EAISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVR 609
+ I P+ SDNR T F +SGTSM+CPHV+GL LL+ HPDWSP+AI+SA++T++
Sbjct: 547 DRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTV 606
Query: 610 DNTLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKT 669
DN+ PM+D + + ++ YGSGH++PT A+DPGLVYD++ DY+ FLC S Y
Sbjct: 607 DNSGEPMMDESTGN--TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTN 666
Query: 670 IRAFSDEPFKCP---PASSVLNLNYPSIGV-----QNLKGSVSVTRKLKNVG-SPGVYRA 729
I + C A V NLNYPS V K S R + NVG S VY
Sbjct: 667 IVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEI 726
Query: 730 QILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVVPK-----DRVGYGALIWSDGRHVV 753
+I P G V+V+P L F RVG++ SF + + K V G ++WSDG+ V
Sbjct: 727 KIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNV 770
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| F4JXC5 | 2.9e-246 | 57.22 | Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... | [more] |
| Q9ZSP5 | 8.8e-219 | 51.61 | Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... | [more] |
| I1N462 | 1.9e-176 | 44.51 | Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... | [more] |
| O65351 | 2.6e-154 | 41.98 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| O49607 | 3.0e-150 | 41.57 | Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| TYK15479.1 | 0.0e+00 | 97.60 | subtilisin-like protease SBT5.4 [Cucumis melo var. makuwa] | [more] |
| XP_011648463.1 | 0.0e+00 | 89.03 | subtilisin-like protease SBT5.4 [Cucumis sativus] >KGN64981.1 hypothetical prote... | [more] |
| XP_038895602.1 | 0.0e+00 | 80.36 | subtilisin-like protease SBT5.4 [Benincasa hispida] | [more] |
| XP_008464322.1 | 0.0e+00 | 76.87 | PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo] | [more] |
| KAE8652826.1 | 0.0e+00 | 77.25 | hypothetical protein Csa_022873 [Cucumis sativus] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A5D3CZ66 | 0.0e+00 | 97.60 | Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... | [more] |
| A0A0A0LYF1 | 0.0e+00 | 89.03 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=3 SV=1 | [more] |
| A0A1S3CLB7 | 0.0e+00 | 76.87 | subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=... | [more] |
| A0A0A0LVY8 | 0.0e+00 | 77.33 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1 | [more] |
| A0A6J1CN43 | 0.0e+00 | 74.45 | subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 P... | [more] |
