MELO3C025408 (gene) Melon (DHL92) v4

Overview


Name	MELO3C025408
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	Thiamine thiazole synthase, chloroplastic
Location	chr06: 30000762 .. 30002850 (-)
RNA-Seq Expression	MELO3C025408
Synteny	MELO3C025408

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTATATGTATCAATGTTTGAAATACATGCATAGATAATCTATGCATCATACATGCAAGCGTGTAAGATTGTGTGATATTCTTCAATATCCTCTATAAAACCCTTCTTCCTTCCCCCCTTCTTCCACACACCAAACTACAATCTCCTCTGTTTTCCTCTGCTTCAAAAATGGCTTCCATTGCTTCCACTCTCACCACCAAGCTCCAAATACCTTCTTTACTTCATGACTCCTCTTTCCATGGCACACCTCTCGCCTCACCTCCTTCTCTCCGCCTCAAATCCCCCGCCGCCACATCTCTCACCATCTCCGCCTCCGCCTCTCAGCCTCCCTACGATTTAAACCAATTCAAATTCAACCCTATCAGAGAATCTATCGTCTCTCGGGAGATGACCCGTCGTTACATGACCGATATGATTACCTACGCTGACACTGACGTTATCATCGTCGGCGCTGGCTCCGCTGGGCTCTCTTGCGCTTACGAACTCAGCAAAAACCCCAATATTCGTATTGCCATCATCGAACAATCCGTGAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTAAGAAATTCATTTCCTTTCCATATTTGAATGATGCTCTGTTTTTTTTTTCTGTTTTGTTCTTTTCTTGAAAATAACAAAAATTTGAAAATCCCCCAATCAGCTTTCATGCATGCCGTATTTGGATTTAAAAAATCCTTTACTTTTTAACAGAGATCTGAAATAAAGAAACCCAGAAAGTAAAATCATTAATTTGAGAGGAAATAGTATACAGATCTTGTGATACCTTAAAAAATTGGATTAATTTGCGTCGGAAACAAATATTCTGATCTTGTTCAGCTGATTGATGTTGGTTAAATTTAATGTTTGATAGGTGGTCCGTAAGCCAGCCCATTTATTCTTGGATGAGGTGGGTGTGGAGTATGACGAGCAAGACGACTATGTCGTGATAAAGCACGCGGCTCTGTTCACGTCGACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTTTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGGGTCGTCACCAACTGGGCTCTTGTGTCAATGAACCATGACACCCAGTCCTGCATGGACCCCAATGTGATGGAGGCTAAGGTGGTTGTGAGCTCTTGTGGCCACGATGGACCTTTTGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGATAGTGTCCCGGGAATGAAGGCCCTTGACATGAACACTGCTGAGGATGCCATTGTTCGATTCACTCGAGAGGTCGTTCCTGGCATGATTGTCACCGGCATGGAGGTTGCCGAGATCGATGGAGCTCCAAGAATGGTAAACGAACTAGAGAGATATTACTGCTTTAGCCCGTTTAATAATCATGATACAGTAAAATATAACAGCGTTTAGATGAGTTTACACGCTTCACACTAATAACTTATTTTACTCCGGCGGCTATCTGCATTAGATTACCACCGAAAGATTTTGTTATACCCAAATTTATTAGTCTTTAAACCGTGCCATTCATAGGGGCCAACGTTCGGGGCGATGATGATATCAGGGCAGAAGGCGGCACACTTGGCACTAAAGTCATTGGGAGAGGCGAACAGCATTGGAGGTGAAGGAGAGATGGGAGAAAGAAAGGTTGGACTTGAAGAGGAAGAGTTGCTACTGGCAGCGGGAGAGTCGCCGGAGGTTGCAGACGCTTAAAGTTTTTAATGGCGGGCGGATGTTGATTTGGAAGAAATATTTGCTTTTGGGTTTTATGGTGCTGTTTGTTTGTTTGTAGTGAGTTTTCAGTAAACGAAAAGGGGGGTTGGTTGTGTTAGGCTTTTTTGTGTTTTCATAACACAATGGGGTTTGGGGAAAGACAAAAAATATCAGTTGCCTTTTTCTCTCTCTCTTTCTTTCTCTTTTTATTTTGTTAATAAAAATGCCTTTCCAAAATATCTCTCCTCCCCACCCCATCCAAATTGGTAATGTTGGTCACGTTTAACCCAGCAGAAGAAAACGTAATAAATCTATGATAATTGATTGATGTACA

mRNA sequence

TTATATGTATCAATGTTTGAAATACATGCATAGATAATCTATGCATCATACATGCAAGCGTGTAAGATTGTGTGATATTCTTCAATATCCTCTATAAAACCCTTCTTCCTTCCCCCCTTCTTCCACACACCAAACTACAATCTCCTCTGTTTTCCTCTGCTTCAAAAATGGCTTCCATTGCTTCCACTCTCACCACCAAGCTCCAAATACCTTCTTTACTTCATGACTCCTCTTTCCATGGCACACCTCTCGCCTCACCTCCTTCTCTCCGCCTCAAATCCCCCGCCGCCACATCTCTCACCATCTCCGCCTCCGCCTCTCAGCCTCCCTACGATTTAAACCAATTCAAATTCAACCCTATCAGAGAATCTATCGTCTCTCGGGAGATGACCCGTCGTTACATGACCGATATGATTACCTACGCTGACACTGACGTTATCATCGTCGGCGCTGGCTCCGCTGGGCTCTCTTGCGCTTACGAACTCAGCAAAAACCCCAATATTCGTATTGCCATCATCGAACAATCCGTGAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTGGTCCGTAAGCCAGCCCATTTATTCTTGGATGAGGTGGGTGTGGAGTATGACGAGCAAGACGACTATGTCGTGATAAAGCACGCGGCTCTGTTCACGTCGACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTTTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGGGTCGTCACCAACTGGGCTCTTGTGTCAATGAACCATGACACCCAGTCCTGCATGGACCCCAATGTGATGGAGGCTAAGGTGGTTGTGAGCTCTTGTGGCCACGATGGACCTTTTGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGATAGTGTCCCGGGAATGAAGGCCCTTGACATGAACACTGCTGAGGATGCCATTGTTCGATTCACTCGAGAGGTCGTTCCTGGCATGATTGTCACCGGCATGGAGGTTGCCGAGATCGATGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCGATGATGATATCAGGGCAGAAGGCGGCACACTTGGCACTAAAGTCATTGGGAGAGGCGAACAGCATTGGAGGTGAAGGAGAGATGGGAGAAAGAAAGGTTGGACTTGAAGAGGAAGAGTTGCTACTGGCAGCGGGAGAGTCGCCGGAGGTTGCAGACGCTTAAAGTTTTTAATGGCGGGCGGATGTTGATTTGGAAGAAATATTTGCTTTTGGGTTTTATGGTGCTGTTTGTTTGTTTGTAGTGAGTTTTCAGTAAACGAAAAGGGGGGTTGGTTGTGTTAGGCTTTTTTGTGTTTTCATAACACAATGGGGTTTGGGGAAAGACAAAAAATATCAGTTGCCTTTTTCTCTCTCTCTTTCTTTCTCTTTTTATTTTGTTAATAAAAATGCCTTTCCAAAATATCTCTCCTCCCCACCCCATCCAAATTGGTAATGTTGGTCACGTTTAACCCAGCAGAAGAAAACGTAATAAATCTATGATAATTGATTGATGTACA

Coding sequence (CDS)

ATGGCTTCCATTGCTTCCACTCTCACCACCAAGCTCCAAATACCTTCTTTACTTCATGACTCCTCTTTCCATGGCACACCTCTCGCCTCACCTCCTTCTCTCCGCCTCAAATCCCCCGCCGCCACATCTCTCACCATCTCCGCCTCCGCCTCTCAGCCTCCCTACGATTTAAACCAATTCAAATTCAACCCTATCAGAGAATCTATCGTCTCTCGGGAGATGACCCGTCGTTACATGACCGATATGATTACCTACGCTGACACTGACGTTATCATCGTCGGCGCTGGCTCCGCTGGGCTCTCTTGCGCTTACGAACTCAGCAAAAACCCCAATATTCGTATTGCCATCATCGAACAATCCGTGAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTGGTCCGTAAGCCAGCCCATTTATTCTTGGATGAGGTGGGTGTGGAGTATGACGAGCAAGACGACTATGTCGTGATAAAGCACGCGGCTCTGTTCACGTCGACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTTTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGGGTCGTCACCAACTGGGCTCTTGTGTCAATGAACCATGACACCCAGTCCTGCATGGACCCCAATGTGATGGAGGCTAAGGTGGTTGTGAGCTCTTGTGGCCACGATGGACCTTTTGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGATAGTGTCCCGGGAATGAAGGCCCTTGACATGAACACTGCTGAGGATGCCATTGTTCGATTCACTCGAGAGGTCGTTCCTGGCATGATTGTCACCGGCATGGAGGTTGCCGAGATCGATGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCGATGATGATATCAGGGCAGAAGGCGGCACACTTGGCACTAAAGTCATTGGGAGAGGCGAACAGCATTGGAGGTGAAGGAGAGATGGGAGAAAGAAAGGTTGGACTTGAAGAGGAAGAGTTGCTACTGGCAGCGGGAGAGTCGCCGGAGGTTGCAGACGCTTAA

Protein sequence

MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA

Homology

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BLAST of MELO3C025408 vs. NCBI nr
Match: XP_008463440.1 (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo])

HSP 1 Score: 697.2 bits (1798), Expect = 7.4e-197
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0


Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60
           MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 359

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BLAST of MELO3C025408 vs. NCBI nr
Match: XP_004152916.1 (thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothetical protein Csa_011133 [Cucumis sativus])

HSP 1 Score: 684.5 bits (1765), Expect = 5.0e-193
Identity = 353/359 (98.33%), Postives = 355/359 (98.89%), Query Frame = 0


Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60
           MASIASTLTTKLQ PSLLHDSSFHGTPLASP SLRLKS AATSLTISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPIRESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGEANSIG EGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 359

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BLAST of MELO3C025408 vs. NCBI nr
Match: XP_038905461.1 (thiamine thiazole synthase, chloroplastic [Benincasa hispida])

HSP 1 Score: 646.4 bits (1666), Expect = 1.5e-181
Identity = 342/363 (94.21%), Postives = 350/363 (96.42%), Query Frame = 0


Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKS----PAATSLTISASASQPPYD 60
           MASIASTLTTKLQ PSLL DSSF+GTPLA PPSLRLKS     AATSL+ISASASQPPYD
Sbjct: 1   MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSLRLKSTTTTTAATSLSISASASQPPYD 60

Query: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAI 120
           LNQFKFNPI+ESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEV 360
           APRMGPTFGAMMISGQKAAHLALKSLGEAN I  +GE+GERKV +EE ELLLAAGESPEV
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANGI-EDGEVGERKV-VEEPELLLAAGESPEV 360

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BLAST of MELO3C025408 vs. NCBI nr
Match: XP_022985241.1 (thiamine thiazole synthase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 608.6 bits (1568), Expect = 3.5e-170
Identity = 319/360 (88.61%), Postives = 338/360 (93.89%), Query Frame = 0


Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLK-SPAATSLTISASASQPPYDLNQ 60
           MASIAS+L TKL  PSLL DSSFHG+PL   PS RLK + AA SL+ISASA+QPPYDLNQ
Sbjct: 1   MASIASSLPTKLHRPSLL-DSSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPYDLNQ 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKFNPIRESIVSREMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSK+P++R+AIIEQ
Sbjct: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQ+DYVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKAAHLALK+LG+ANSI  +GE G RKV  E  E++LAAGE+PEVADA
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGKANSIDEKGENGGRKV--EAAEMVLAAGETPEVADA 357

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BLAST of MELO3C025408 vs. NCBI nr
Match: XP_023520449.1 (thiamine thiazole synthase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 602.4 bits (1552), Expect = 2.5e-168
Identity = 314/360 (87.22%), Postives = 338/360 (93.89%), Query Frame = 0


Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLK-SPAATSLTISASASQPPYDLNQ 60
           MASIAS+L TKL  PSLL +SSFHG+PL   PS RLK + AA SL+ISASA+QPPYDLNQ
Sbjct: 1   MASIASSLPTKLHRPSLL-ESSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPYDLNQ 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKFNPIRESIVSREMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSK+P++R+AIIEQ
Sbjct: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQ+DYVVIKHAALFTSTIMSKLL+
Sbjct: 121 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLS 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+IDSVPGMKALDMN+AEDAIVRFTREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNSAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKAAHLALK+LG+ANSI  +GE G R+V  E  E++LAAGE+PEVADA
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGKANSIDEKGENGGREV--EAAEMVLAAGETPEVADA 357

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BLAST of MELO3C025408 vs. ExPASy Swiss-Prot
Match: O23787 (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=2 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 1.6e-157
Identity = 296/364 (81.32%), Postives = 321/364 (88.19%), Query Frame = 0


Query: 1   MASIAST--LTTKLQIPSLLHDSSFHGTPLASPPSLRL---KSPAATSLTISASASQPPY 60
           MA++AST    +     + L DSSFHG P+ SP  LRL   KS    +L+ISASAS PPY
Sbjct: 1   MAAMASTAFAPSVSSTTNKLFDSSFHGAPM-SPSLLRLQPIKSSRPNNLSISASAS-PPY 60

Query: 61  DLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIA 120
           DLN FKF+PI+ESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNPNI+IA
Sbjct: 61  DLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH+FLDE+G++YDEQD+YVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIG+I+ VPGMKALDMN+AEDAIVR TREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPE 360
           GAPRMGPTFGAMMISGQKAAHLALKSLG+ N++ G        VG    EL+LAA +S E
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDG------TYVGGVHPELILAAADSAE 356

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BLAST of MELO3C025408 vs. ExPASy Swiss-Prot
Match: Q38709 (Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=2 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 5.4e-150
Identity = 282/361 (78.12%), Postives = 316/361 (87.53%), Query Frame = 0


Query: 4   IASTLTTKLQIPSLLHD--SSFHGTPLASPPSLRL---KSPAATSLTISASASQPPYDLN 63
           +ASTLT+K Q  +L  +  SSF+GTPLA P S+R+   K+ A  S+++S + S PPYDL 
Sbjct: 1   MASTLTSKPQRLALFENSASSFYGTPLA-PSSIRVQPTKAGAKPSISMSGAPS-PPYDLK 60

Query: 64  QFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIE 123
            F F+PI+ESIVSREMTRRYM DMITYADTDV++VGAGS+GL C YELSKNP++++AIIE
Sbjct: 61  AFTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQVAIIE 120

Query: 124 QSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL 183
           QSVSPGGGAWLGGQLFS MVVRKPAHLFLDE+G+EYDEQD+YVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLL 180

Query: 184 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 243
           ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240

Query: 244 GPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAP 303
           GPFGATGVK L+SIG+ID+VPGMKALDMN AEDAIVR TRE+VPGMIVTGMEVAEIDGAP
Sbjct: 241 GPFGATGVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDGAP 300

Query: 304 RMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVAD 360
           RMGPTFGAMMISGQKAAHLALK+LG  N++ G        VG    EL+LAA +S E+AD
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKALGLPNALDGS------YVGGIHPELILAAADSAEIAD 352

[Close]

BLAST of MELO3C025408 vs. ExPASy Swiss-Prot
Match: F6H7K5 (Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 2.1e-149
Identity = 284/365 (77.81%), Postives = 311/365 (85.21%), Query Frame = 0


Query: 1   MASIASTLTTKLQIPS---LLHDSSFHGTPLA---SPPSLRLKSPAATSLTISASASQPP 60
           MAS+A+TLT+    P      + SSFHG+P++    P  +  +SP     TIS S S  P
Sbjct: 1   MASMATTLTSLSSNPKPAFFDNKSSFHGSPISYRVLPIKVSHQSP-----TISMS-SVSP 60

Query: 61  YDLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRI 120
           YDL  FKF PI+ESIV+REMTRRYM DMITYADTDV+IVGAGSAGLSCAYELSKNP+IR+
Sbjct: 61  YDLQSFKFEPIKESIVAREMTRRYMMDMITYADTDVVIVGAGSAGLSCAYELSKNPSIRV 120

Query: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIM 180
           AIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQD+YVVIKHAALFTSTIM
Sbjct: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHHFLDELGIEYDEQDNYVVIKHAALFTSTIM 180

Query: 181 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240
           SKLLARPNVKLFNAVAAEDLIVK  RV GVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS
Sbjct: 181 SKLLARPNVKLFNAVAAEDLIVKEERVAGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240

Query: 241 CGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEI 300
           CGHDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TRE+VPGMIVTGMEVAEI
Sbjct: 241 CGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEI 300

Query: 301 DGAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESP 360
           DGAPRMGPTFGAMMISGQKAAHLAL++LG+ N+I G     E      + EL+LAA E+ 
Sbjct: 301 DGAPRMGPTFGAMMISGQKAAHLALRALGQPNAIDGNYTEAETM----QPELILAAAETG 355

[Close]

BLAST of MELO3C025408 vs. ExPASy Swiss-Prot
Match: F6H9A9 (Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 1.7e-148
Identity = 272/360 (75.56%), Postives = 311/360 (86.39%), Query Frame = 0


Query: 1   MASIASTLTTKLQIPSL-LHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQ 60
           MA++ S++ +K +      H SSFHG P+A+    RL    +T + ++ +A+  PYDL  
Sbjct: 1   MATLTSSICSKPKASVFDPHKSSFHGVPIAT--QARLSPVKSTPVNLAVTAAAMPYDLRS 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKF PI+ESIVSREMTRRYM DMITYADTDV++VGAGSAGLSCAYELSKNP++++AIIEQ
Sbjct: 61  FKFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFS+MVVRKPAH FLDE+G+EYDEQD+YVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSSMVVRKPAHRFLDELGLEYDEQDNYVVIKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLI+K G+VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIIKEGKVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRL+S+G+IDSVPGMKALDMNTAED IVR TREVVPGMIVTGMEVAEIDG+PR
Sbjct: 241 PFGATGVKRLRSVGMIDSVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDGSPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKAAHLALKSLG  N++ G   +G     L  E +L AA ++ E+A+A
Sbjct: 301 MGPTFGAMMISGQKAAHLALKSLGLPNALDGT-YIG----NLHPELVLAAAADAAEIAEA 353

[Close]

BLAST of MELO3C025408 vs. ExPASy Swiss-Prot
Match: Q38814 (Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI1 PE=1 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 1.7e-148
Identity = 277/360 (76.94%), Postives = 304/360 (84.44%), Query Frame = 0


Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPL-ASPPSLRLKSPAATSLTISASASQPPYDLNQ 60
           MA+IASTL+     P  L DSSFHG+ + A+P S+ LK       + S  A+   YDLN 
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAPISIGLK-----PRSFSVRATTAGYDLNA 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           F F+PI+ESIVSREMTRRYMTDMITYA+TDV++VGAGSAGLS AYE+SKNPN+++AIIEQ
Sbjct: 61  FTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAM+VRKPAHLFLDE+GV YDEQD YVV+KHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+ID VPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKA  LALK+LG  N+I G        VG    EL+LAA +S E  DA
Sbjct: 301 MGPTFGAMMISGQKAGQLALKALGLPNAIDG------TLVGNLSPELVLAAADSAETVDA 349

[Close]

BLAST of MELO3C025408 vs. ExPASy TrEMBL
Match: A0A1S3CJ81 (Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501605 PE=3 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 3.6e-197
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0


Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60
           MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]

BLAST of MELO3C025408 vs. ExPASy TrEMBL
Match: A0A0A0L546 (Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=3 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 2.4e-193
Identity = 353/359 (98.33%), Postives = 355/359 (98.89%), Query Frame = 0


Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60
           MASIASTLTTKLQ PSLLHDSSFHGTPLASP SLRLKS AATSLTISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPIRESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGEANSIG EGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]

BLAST of MELO3C025408 vs. ExPASy TrEMBL
Match: A0A6J1J7L4 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483293 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.7e-170
Identity = 319/360 (88.61%), Postives = 338/360 (93.89%), Query Frame = 0


Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLK-SPAATSLTISASASQPPYDLNQ 60
           MASIAS+L TKL  PSLL DSSFHG+PL   PS RLK + AA SL+ISASA+QPPYDLNQ
Sbjct: 1   MASIASSLPTKLHRPSLL-DSSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPYDLNQ 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKFNPIRESIVSREMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSK+P++R+AIIEQ
Sbjct: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQ+DYVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKAAHLALK+LG+ANSI  +GE G RKV  E  E++LAAGE+PEVADA
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGKANSIDEKGENGGRKV--EAAEMVLAAGETPEVADA 357

[Close]

BLAST of MELO3C025408 vs. ExPASy TrEMBL
Match: A0A6J1EVU2 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438464 PE=3 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.5e-166
Identity = 311/360 (86.39%), Postives = 332/360 (92.22%), Query Frame = 0


Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLK-SPAATSLTISASASQPPYDLNQ 60
           MASIAS+L  KL  PSLL +SSFHG+PL   PS RLK + AA SL+ISASA+QPPYDLNQ
Sbjct: 1   MASIASSLPIKLHRPSLL-ESSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPYDLNQ 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKFNPIRESIVSREMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSK+P++R+AIIEQ
Sbjct: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQ+DYVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+IDSVPGMKALDMN+AEDAIVRFTREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNSAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKAAHLALK+LG+ NSI GE         +E  E++LAAGE+PEVADA
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGKTNSIDGE---------VEAAEMVLAAGETPEVADA 350

[Close]

BLAST of MELO3C025408 vs. ExPASy TrEMBL
Match: A0A6J1FWL6 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448035 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.6e-165
Identity = 317/361 (87.81%), Postives = 327/361 (90.58%), Query Frame = 0


Query: 1   MASIASTLTTKLQIP-SLLHDSSFHGTPLA-SPPSLRLKS-PAATSLTISASASQPPYDL 60
           MAS+ASTLTTKLQ P S L DSSFHGTPLA  PPSLRLKS PAA S++ S    Q PYDL
Sbjct: 1   MASVASTLTTKLQTPSSSLLDSSFHGTPLAPPPPSLRLKSTPAAFSISAS---QQQPYDL 60

Query: 61  NQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAII 120
           N FKFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP+IRIAII
Sbjct: 61  NSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAII 120

Query: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180
           EQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKL 180

Query: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240
           LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240

Query: 241 DGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300
           DGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA 300

Query: 301 PRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVA 359
           PRMGPTFGAMMISGQKAAHLALKSLG AN I  E             +L+LAAGESPEVA
Sbjct: 301 PRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA-----------ADLILAAGESPEVA 347

[Close]

BLAST of MELO3C025408 vs. TAIR 10
Match: AT5G54770.1 (thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) )

HSP 1 Score: 526.9 bits (1356), Expect = 1.2e-149
Identity = 277/360 (76.94%), Postives = 304/360 (84.44%), Query Frame = 0


Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPL-ASPPSLRLKSPAATSLTISASASQPPYDLNQ 60
           MA+IASTL+     P  L DSSFHG+ + A+P S+ LK       + S  A+   YDLN 
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAPISIGLK-----PRSFSVRATTAGYDLNA 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           F F+PI+ESIVSREMTRRYMTDMITYA+TDV++VGAGSAGLS AYE+SKNPN+++AIIEQ
Sbjct: 61  FTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAM+VRKPAHLFLDE+GV YDEQD YVV+KHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+ID VPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKA  LALK+LG  N+I G        VG    EL+LAA +S E  DA
Sbjct: 301 MGPTFGAMMISGQKAGQLALKALGLPNAIDG------TLVGNLSPELVLAAADSAETVDA 349

The following BLAST results are available for this feature:

BLAST of MELO3C025408 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008463440.1	7.4e-197	100.00	PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo]	[more]
XP_004152916.1	5.0e-193	98.33	thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothet...	[more]
XP_038905461.1	1.5e-181	94.21	thiamine thiazole synthase, chloroplastic [Benincasa hispida]	[more]
XP_022985241.1	3.5e-170	88.61	thiamine thiazole synthase, chloroplastic-like [Cucurbita maxima]	[more]
XP_023520449.1	2.5e-168	87.22	thiamine thiazole synthase, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MELO3C025408 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23787	1.6e-157	81.32	Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=...	[more]
Q38709	5.4e-150	78.12	Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=...	[more]
F6H7K5	2.1e-149	77.81	Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2...	[more]
F6H9A9	1.7e-148	75.56	Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1...	[more]
Q38814	1.7e-148	76.94	Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI...	[more]

BLAST of MELO3C025408 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CJ81	3.6e-197	100.00	Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10350160...	[more]
A0A0A0L546	2.4e-193	98.33	Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=...	[more]
A0A6J1J7L4	1.7e-170	88.61	Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1EVU2	2.5e-166	86.39	Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1FWL6	1.6e-165	87.81	Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of MELO3C025408 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54770.1	1.2e-149	76.94	thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00411	PNDRDTASEI	coord: 344..358 score: 28.63 coord: 89..111 score: 30.31
None	No IPR available	PFAM	PF01946	Thi4	coord: 71..304 e-value: 1.2E-114 score: 381.4
None	No IPR available	GENE3D	6.10.250.2840		coord: 56..86 e-value: 4.6E-19 score: 69.8
None	No IPR available	PANTHER	PTHR43422	THIAMINE THIAZOLE SYNTHASE	coord: 1..359
None	No IPR available	PANTHER	PTHR43422:SF11	THIAMINE THIAZOLE SYNTHASE, CHLOROPLASTIC	coord: 1..359
IPR002922	Thiazole biosynthetic enzyme Thi4 family	TIGRFAM	TIGR00292	TIGR00292	coord: 67..322 e-value: 1.8E-82 score: 274.4
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 87..322 e-value: 3.8E-122 score: 408.0
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 47..316
IPR027495	Thiamine thiazole synthase	HAMAP	MF_03158	THI4	coord: 5..331 score: 68.31456

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C025408.1	MELO3C025408.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009228	thiamine biosynthetic process