MELO3C024346 (gene) Melon (DHL92) v4

BLAST of MELO3C024346 vs. NCBI nr
Match: XP_008462294.1 (PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease SBT1.4 [Cucumis melo])

HSP 1 Score: 1531.5 bits (3964), Expect = 0.0e+00
Identity = 769/770 (99.87%), Postives = 769/770 (99.87%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180
           LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180

Query: 181 NRKIIGARAFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAFF GYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFXGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360
           IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420
           SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540
           IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN
Sbjct: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600

Query: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660
           FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN
Sbjct: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE 720
           PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE
Sbjct: 661 PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE 720

Query: 721 KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 771
           KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM
Sbjct: 721 KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 770

BLAST of MELO3C024346 vs. NCBI nr
Match: TYK03935.1 (subtilisin-like protease SBT1.4 [Cucumis melo var. makuwa])

HSP 1 Score: 1490.3 bits (3857), Expect = 0.0e+00
Identity = 745/748 (99.60%), Postives = 748/748 (100.00%), Query Frame = 0

Query: 23  VFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTKLLYNYERAANGFS 82
           +FSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPT+LLYNYERAANGFS
Sbjct: 23  LFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTRLLYNYERAANGFS 82

Query: 83  ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 142
           ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL
Sbjct: 83  ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 142

Query: 143 DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL 202
           DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL
Sbjct: 143 DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL 202

Query: 203 KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG 262
           KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG
Sbjct: 203 KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG 262

Query: 263 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS 322
           CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS
Sbjct: 263 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS 322

Query: 323 GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG 382
           GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG
Sbjct: 323 GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG 382

Query: 383 DCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 442
           DCGSRYCYSGSLDSSKVAG+IVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA
Sbjct: 383 DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 442

Query: 443 DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT 502
           DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT
Sbjct: 443 DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT 502

Query: 503 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 562
           AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA
Sbjct: 503 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 562

Query: 563 FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL 622
           FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL
Sbjct: 563 FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL 622

Query: 623 TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT 682
           TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT
Sbjct: 623 TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT 682

Query: 683 RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF 742
           RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF
Sbjct: 683 RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF 742

Query: 743 GSIQWSDGIHNVRSPIAVSFKTGSIASM 771
           GSIQWSDGIHNVRSPIAVSFKTGSIASM
Sbjct: 743 GSIQWSDGIHNVRSPIAVSFKTGSIASM 770

BLAST of MELO3C024346 vs. NCBI nr
Match: KAA0059392.1 (subtilisin-like protease SBT1.4 [Cucumis melo var. makuwa])

HSP 1 Score: 1490.3 bits (3857), Expect = 0.0e+00
Identity = 745/748 (99.60%), Postives = 748/748 (100.00%), Query Frame = 0

Query: 23  VFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTKLLYNYERAANGFS 82
           +FSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPT+LLYNYERAANGFS
Sbjct: 3   LFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTRLLYNYERAANGFS 62

Query: 83  ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 142
           ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL
Sbjct: 63  ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 122

Query: 143 DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL 202
           DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL
Sbjct: 123 DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL 182

Query: 203 KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG 262
           KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG
Sbjct: 183 KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG 242

Query: 263 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS 322
           CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS
Sbjct: 243 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS 302

Query: 323 GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG 382
           GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG
Sbjct: 303 GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG 362

Query: 383 DCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 442
           DCGSRYCYSGSLDSSKVAG+IVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA
Sbjct: 363 DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 422

Query: 443 DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT 502
           DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT
Sbjct: 423 DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT 482

Query: 503 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 562
           AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA
Sbjct: 483 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 542

Query: 563 FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL 622
           FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL
Sbjct: 543 FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL 602

Query: 623 TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT 682
           TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT
Sbjct: 603 TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT 662

Query: 683 RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF 742
           RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF
Sbjct: 663 RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF 722

Query: 743 GSIQWSDGIHNVRSPIAVSFKTGSIASM 771
           GSIQWSDGIHNVRSPIAVSFKTGSIASM
Sbjct: 723 GSIQWSDGIHNVRSPIAVSFKTGSIASM 750

BLAST of MELO3C024346 vs. NCBI nr
Match: XP_004141706.1 (subtilisin-like protease SBT1.4 [Cucumis sativus] >KGN45480.1 hypothetical protein Csa_016246 [Cucumis sativus])

HSP 1 Score: 1462.2 bits (3784), Expect = 0.0e+00
Identity = 733/771 (95.07%), Postives = 750/771 (97.28%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MANFHLPSSSFFFFF+   FPL+FSRSLE QETYIVHVSKSEKPSLFSSHHHWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120
           LS SPHPTKLLYNYERAANGFSARITT QAEELRRVPGIISVIPD+IRQLHTTRTPHFLG
Sbjct: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180
           LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGL+PVPARWKGTCDTGEG SA AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180

Query: 181 NRKIIGARAFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F+GYESNLRGSLK SSDFKSARDT+GHGTHTASTAAGSFV NASFFQYAR
Sbjct: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420
           SGVSLYSGDPLGDSKL LVYGGDCGSRYCYSGSLDSSKVAG+IVVCDRGGNARVAKGGAV
Sbjct: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVG IAGNKLRDYIHTDPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540
           IGDSPPAP+VA+FSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSG PIKDL+TSEESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600

Query: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660
           FVHGAGHI+PNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSY QLCEHKL+N
Sbjct: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660

Query: 661 PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE 720
           PGNLNYPSFSVVFD+ EVVKYTRTVTNVGDETEVVY VKVEAPQGV ISVVPNKLEFNKE
Sbjct: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720

Query: 721 KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKT-GSIASM 771
           KTT SYEITFTKINGFKESASFGSIQW DGIH+VRSPIAVSFKT GSIASM
Sbjct: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771

BLAST of MELO3C024346 vs. NCBI nr
Match: XP_038897980.1 (subtilisin-like protease SBT1.4 [Benincasa hispida])

HSP 1 Score: 1392.9 bits (3604), Expect = 0.0e+00
Identity = 696/770 (90.39%), Postives = 729/770 (94.68%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA  HL  SS FF F+L   P++FSRS E QET+IVHVSKSEKPSLFSSHHHWHSSILES
Sbjct: 1   MAKSHL-CSSLFFLFILCFAPVIFSRSPENQETFIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLYNYERA NGFSAR+T AQA +LR VPG+ISVIPDRIRQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAVNGFSARLTAAQAAQLRHVPGVISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180
           LADNLGLW DTNYADDVIIGVLDTGIWPERPSFSDEGL+PVPA WKGTC+TGEG SA  C
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASFC 180

Query: 181 NRKIIGARAFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F+GY SN   S  GSSDFKSARDT+GHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAYFYGYASNFGRSFNGSSDFKSARDTEGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGC+DSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360
           IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420
           SGVSLYSGDPLGDSK+PLVYGGDCGSRYCYSGSL+ SKVAG+IVVCDRGGNARVAKGGAV
Sbjct: 361 SGVSLYSGDPLGDSKVPLVYGGDCGSRYCYSGSLNPSKVAGKIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIH+DPNPTA IVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHSDPNPTAIIVFRGTV 480

Query: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540
           IGDSPPAP+VA+FSSRGPNYRTAEILKPDVIAPGVNILA WSGYSSPTGLNIDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILASWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDL+TSEESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSEESNP 600

Query: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660
           FVHGAGHIDPN+ALNPGLIYD++PQDYVSFLCSIGYDSKQIAVFVK SSYSQLCEHKLSN
Sbjct: 601 FVHGAGHIDPNKALNPGLIYDISPQDYVSFLCSIGYDSKQIAVFVKDSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE 720
           PGNLNYPSF+VVF+  +VVKYTRTVTNVGDE++VVY VKVEAPQGVEISVVPNKL FN E
Sbjct: 661 PGNLNYPSFAVVFNGEDVVKYTRTVTNVGDESDVVYEVKVEAPQGVEISVVPNKLSFNAE 720

Query: 721 KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 771
           KTT SYEITFTKI+GF E+ASFGSIQWSDG HNVRSPIAVSF TGSIASM
Sbjct: 721 KTTDSYEITFTKISGFVEAASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769

BLAST of MELO3C024346 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 6.1e-284
Identity = 492/770 (63.90%), Postives = 595/770 (77.27%), Query Frame = 0

Query: 8   SSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHP 67
           SS FF F +L  F    S S +  E+YIVHV +S KPSLFSSH++WH S+L SL  SP P
Sbjct: 7   SSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQP 66

Query: 68  TKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGL 127
             LLY+Y RA +GFSAR++  Q   LRR P +ISVIPD+ R++HTT TP FLG + N GL
Sbjct: 67  ATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGL 126

Query: 128 WADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGA 187
           W+++NY +DVI+GVLDTGIWPE PSFSD GL P+P+ WKG C+ G    A +CNRK+IGA
Sbjct: 127 WSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGA 186

Query: 188 RAFFFGYESNLRGSLK-GSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGM 247
           RAF+ GY +   G+ K  + + +S RDT+GHGTHTASTAAGS V NAS +QYARG A GM
Sbjct: 187 RAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGM 246

Query: 248 ASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAF 307
           AS+ARIAAYKICW  GCYDSDILAAMDQA++DGV VISLSVG+SG AP Y+ DSIAIGAF
Sbjct: 247 ASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAF 306

Query: 308 GAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLY 367
           GA +HGIVVSCSAGNSGP P TA NIAPWI+TVGAST+DREF A+ I GD +VF+G SLY
Sbjct: 307 GATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLY 366

Query: 368 SGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAVKSAGGL 427
           +G+ L DS+L LVY GDCGSR CY G L+SS V G+IV+CDRGGNARV KG AVK AGG 
Sbjct: 367 AGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGA 426

Query: 428 GMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPP 487
           GM+LANT E+GEEL ADSHL+P TMVG  AG+++RDYI T  +PTA I F GT+IG SPP
Sbjct: 427 GMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPP 486

Query: 488 APKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTS 547
           +P+VA+FSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L+IDPRRV+FNIISGTS
Sbjct: 487 SPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTS 546

Query: 548 MSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAG 607
           MSCPHVSG+AALLRKA P WSPAAIKSAL+TT+Y +++SG PI+DLAT + SN F+HGAG
Sbjct: 547 MSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAG 606

Query: 608 HIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCE-HKLSNPGNLN 667
           H+DPN+ALNPGL+YD+  ++YV+FLC++GY+   I VF++  +    CE  KL   G+LN
Sbjct: 607 HVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLN 666

Query: 668 YPSFSVVF-DDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTL 727
           YPSFSVVF   GEVVKY R V NVG   + VY V V++P  VEI V P+KL F+KEK+ L
Sbjct: 667 YPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVL 726

Query: 728 SYEITFTKI-----NGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIAS 770
            YE+TF  +      G      FGSI+W+DG H V+SP+AV +  GS+ S
Sbjct: 727 EYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of MELO3C024346 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 1.6e-220
Identity = 416/764 (54.45%), Postives = 528/764 (69.11%), Query Frame = 0

Query: 8   SSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHP 67
           SS+ FF  +   F  V S S + Q TYIVH++KS+ PS F  H +W+ S L S+S S   
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSD-QGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS--- 66

Query: 68  TKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLG- 127
            +LLY YE A +GFS R+T  +A+ L   PG+ISV+P+   +LHTTRTP FLGL ++   
Sbjct: 67  AELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD 126

Query: 128 LWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIG 187
           L+ +     DV++GVLDTG+WPE  S+SDEG  P+P+ WKG C+ G   +A  CNRK+IG
Sbjct: 127 LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIG 186

Query: 188 ARAFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGM 247
           AR F  GYES + G +  S + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGM
Sbjct: 187 ARFFARGYESTM-GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGM 246

Query: 248 ASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAF 307
           A RAR+A YK+CW  GC+ SDILAA+D+AI+D V+V+S+S+G  G    YYRD +AIGAF
Sbjct: 247 APRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAF 306

Query: 308 GAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLY 367
            AM+ GI+VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+ + F+GVSL+
Sbjct: 307 AAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLF 366

Query: 368 SGDPLGDSKLPLVYGGDCGS----RYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAVKS 427
            G+ L D  LP +Y G+  +      C +G+L   KV G+IV+CDRG NARV KG  VK+
Sbjct: 367 KGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKA 426

Query: 428 AGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIG 487
           AGG+GM+LANT  NGEEL+AD+HL+P T VGE AG+ +R Y+ TDPNPTA+I   GTV+G
Sbjct: 427 AGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG 486

Query: 488 DSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNII 547
              P+P VA+FSSRGPN  T  ILKPD+IAPGVNILA W+G + PTGL  D RRVEFNII
Sbjct: 487 -VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNII 546

Query: 548 SGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFV 607
           SGTSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D+AT + S PF 
Sbjct: 547 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFD 606

Query: 608 HGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCE-HKLSNP 667
           HGAGH+ P  A NPGLIYDLT +DY+ FLC++ Y S QI      S  +  C+  K  + 
Sbjct: 607 HGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR---SVSRRNYTCDPSKSYSV 666

Query: 668 GNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEA-PQGVEISVVPNKLEFNKE 727
            +LNYPSF+V  D     KYTRTVT+VG      Y VKV +   GV+ISV P  L F + 
Sbjct: 667 ADLNYPSFAVNVDGVGAYKYTRTVTSVGGAG--TYSVKVTSETTGVKISVEPAVLNFKEA 726

Query: 728 KTTLSYEITFTKINGFKESA--SFGSIQWSDGIHNVRSPIAVSF 763
               SY +TFT ++  K S   SFGSI+WSDG H V SP+A+S+
Sbjct: 727 NEKKSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of MELO3C024346 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 4.6e-215
Identity = 399/760 (52.50%), Postives = 514/760 (67.63%), Query Frame = 0

Query: 8   SSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHP 67
           SSS       F+F L+ + +   ++TYI+ V+ S+KP  F +HH W++S L S S     
Sbjct: 7   SSSSITIITTFLFLLLHTTA---KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES----- 66

Query: 68  TKLLYNYERAANGFSARITTAQAEE-LRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLG 127
             LLY Y  + +GFSA + + +A+  L     I+ +  D +  LHTTRTP FLGL    G
Sbjct: 67  -SLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFG 126

Query: 128 LWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIG 187
           +    + ++ VIIGVLDTG+WPE  SF D  +  +P++WKG C++G    +  CN+K+IG
Sbjct: 127 VHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIG 186

Query: 188 ARAFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGM 247
           AR+F  G++    G      +  S RD DGHGTHT++TAAGS V+NASF  YA G ARGM
Sbjct: 187 ARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGM 246

Query: 248 ASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAF 307
           A+RAR+A YK+CW  GC+ SDILAAMD+AI DGVDV+SLS+G  G AP YYRD+IAIGAF
Sbjct: 247 ATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLG-GGSAP-YYRDTIAIGAF 306

Query: 308 GAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLY 367
            AM+ G+ VSCSAGNSGP   +  N+APW++TVGA T+DR+F A   LG+ +  +GVSLY
Sbjct: 307 SAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLY 366

Query: 368 SGDPLGDSKLPLVY--GGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAVKSAG 427
           SG  +G   L LVY  G    S  C  GSLDSS V G+IVVCDRG NARV KG  V+ AG
Sbjct: 367 SGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAG 426

Query: 428 GLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDS 487
           GLGM++ANT  +GEEL+ADSHL+P   VG+  G+ LR+Y+ +D  PTA +VF+GTV+ D 
Sbjct: 427 GLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DV 486

Query: 488 PPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISG 547
            P+P VA+FSSRGPN  T EILKPDVI PGVNILAGWS    PTGL+ D RR +FNI+SG
Sbjct: 487 KPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSG 546

Query: 548 TSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHG 607
           TSMSCPH+SG+A LL+ A P WSP+AIKSAL+TT+Y LD++ +P+ D A +  SNP+ HG
Sbjct: 547 TSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHG 606

Query: 608 AGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNL 667
           +GH+DP +AL+PGL+YD++ ++Y+ FLCS+ Y    I   VK  S +  C  K S+PG L
Sbjct: 607 SGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQL 666

Query: 668 NYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTL 727
           NYPSFSV+F    VV+YTR VTNVG  +  VY V V     V ISV P+KL F       
Sbjct: 667 NYPSFSVLFGGKRVVRYTREVTNVGAASS-VYKVTVNGAPSVGISVKPSKLSFKSVGEKK 726

Query: 728 SYEITFTKING--FKESASFGSIQWSDGIHNVRSPIAVSF 763
            Y +TF    G      A FGSI WS+  H VRSP+A S+
Sbjct: 727 RYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of MELO3C024346 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 2.9e-209
Identity = 393/778 (50.51%), Postives = 519/778 (66.71%), Query Frame = 0

Query: 11  FFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTKL 70
           F+FFF+L +     S S     TYIVHV    KPS+F +H HW++S L SL+ S  P  +
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPSI 64

Query: 71  LYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGL--ADNLGLW 130
           ++ Y+   +GFSAR+T+  A +L   P +ISVIP+++R LHTTR+P FLGL   D  GL 
Sbjct: 65  IHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLL 124

Query: 131 ADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGAR 190
            ++++  D++IGV+DTG+WPERPSF D GL PVP +WKG C   +     ACNRK++GAR
Sbjct: 125 EESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGAR 184

Query: 191 AFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMAS 250
            F  GYE+   G +  +++F+S RD+DGHGTHTAS +AG +V  AS   YA G A GMA 
Sbjct: 185 FFCGGYEAT-NGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAP 244

Query: 251 RARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGA 310
           +AR+AAYK+CW  GCYDSDILAA D A++DGVDVISLSVG  G    YY D+IAIGAFGA
Sbjct: 245 KARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGA 304

Query: 311 MQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSG 370
           +  GI VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+ ++ SGVS+Y G
Sbjct: 305 IDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGG 364

Query: 371 DPLGDSKL-PLVYGGDC------GSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAVK 430
             L   ++ PLVYGG         S  C  GSLD + V G+IV+CDRG N+R  KG  V+
Sbjct: 365 PGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVR 424

Query: 431 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYI------HTDPNPTATIV 490
             GGLGM++AN   +GE L+AD H++P T VG   G+++R YI       +  +PTATIV
Sbjct: 425 KNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIV 484

Query: 491 FRGTVIGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPR 550
           F+GT +G   PAP VASFS+RGPN  T EILKPDVIAPG+NILA W     P+G+  D R
Sbjct: 485 FKGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNR 544

Query: 551 RVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATS 610
           R EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y++D+SG P+ D +T 
Sbjct: 545 RTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTG 604

Query: 611 EESNPFVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCE 670
             S+   +G+GH+ P +A++PGL+YD+T  DY++FLC+  Y    I    +  +    C+
Sbjct: 605 NTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD---CD 664

Query: 671 --HKLSNPGNLNYPSFSVVFDD-GE---VVKYTRTVTNVGDETEVVYGVKVEAPQGVEIS 730
              +  + GNLNYPSFSVVF   GE      + RTVTNVGD ++ VY +K+  P+G  ++
Sbjct: 665 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVT 724

Query: 731 VVPNKLEFNKEKTTLSYEI----TFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFK 764
           V P KL F +    LS+ +    T  K++    +   G I WSDG  NV SP+ V+ +
Sbjct: 725 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of MELO3C024346 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.0e-201
Identity = 374/774 (48.32%), Postives = 509/774 (65.76%), Query Frame = 0

Query: 13  FFFVLFVFPLVF-----SRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLS----- 72
           F F++    L+F     +  + T++TY++H+ KS  P  +++H  W+SS + S++     
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 73  -PSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGL 132
               +  ++LY Y+ A +G +A++T  +AE L    G+++VIP+   +LHTTR+P FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 133 --ADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALA 192
              ++  +WA+     DV++GVLDTGIWPE  SF+D G++PVPA W+G C+TG+      
Sbjct: 131 ERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRN 190

Query: 193 CNRKIIGARAFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYA 252
           CNRKI+GAR F+ GYE+   G +    ++KS RD DGHGTHTA+T AGS VK A+ F +A
Sbjct: 191 CNRKIVGARVFYRGYEA-ATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFA 250

Query: 253 RGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRD 312
            G ARGMA +AR+AAYK+CW  GC+ SDIL+A+DQA++DGV V+S+S+G  G    Y RD
Sbjct: 251 YGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLG--GGVSTYSRD 310

Query: 313 SIAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRV 372
           S++I  FGAM+ G+ VSCSAGN GP P +  N++PWI TVGAST+DR+F A V +G  R 
Sbjct: 311 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 370

Query: 373 FSGVSLYSGDPL--GDSKLPLVYGGDCGS-----RYCYSGSLDSSKVAGRIVVCDRGGNA 432
           F GVSLY G  +   + + PLVY G   S      +C  G+LD   VAG+IV+CDRG   
Sbjct: 371 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 430

Query: 433 RVAKGGAVKSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTA 492
           RV KG  VK AGG+GMVL NT  NGEEL+ADSH++P   VGE  G  ++ Y  T    TA
Sbjct: 431 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATA 490

Query: 493 TIVFRGTVIGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNI 552
           ++   GT IG   P+P VA+FSSRGPN+ + EILKPD++APGVNILA W+G  +P+ L+ 
Sbjct: 491 SLEILGTRIG-IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSS 550

Query: 553 DPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDL 612
           DPRRV+FNI+SGTSMSCPHVSGVAAL++   P WSPAAIKSAL+TT+Y  D+   P+ D 
Sbjct: 551 DPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDA 610

Query: 613 ATSEESNPFVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQ 672
           + +  S+P+ HGAGHIDP +A +PGL+YD+ PQ+Y  FLC+      Q+ VF K S  ++
Sbjct: 611 SGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHS--NR 670

Query: 673 LCEHKLS-NPGNLNYPSFSVVFDDGEVVK---YTRTVTNVGDETEVVYGVKVEAPQGVEI 732
            C+H L+ NPGNLNYP+ S +F +   VK     RTVTNVG      Y V V   +G  +
Sbjct: 671 TCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHIS-SYKVSVSPFKGASV 730

Query: 733 SVVPNKLEFNKEKTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSF 763
           +V P  L F  +   LSY +TF      K    FG + W    H VRSP+ +++
Sbjct: 731 TVQPKTLNFTSKHQKLSYTVTFRTRFRMKR-PEFGGLVWKSTTHKVRSPVIITW 776

BLAST of MELO3C024346 vs. ExPASy TrEMBL
Match: A0A1S3CGN8 (LOW QUALITY PROTEIN: subtilisin-like protease SBT1.4 OS=Cucumis melo OX=3656 GN=LOC103500685 PE=3 SV=1)

HSP 1 Score: 1531.5 bits (3964), Expect = 0.0e+00
Identity = 769/770 (99.87%), Postives = 769/770 (99.87%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180
           LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180

Query: 181 NRKIIGARAFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAFF GYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFXGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360
           IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420
           SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540
           IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN
Sbjct: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600

Query: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660
           FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN
Sbjct: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE 720
           PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE
Sbjct: 661 PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE 720

Query: 721 KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 771
           KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM
Sbjct: 721 KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 770

BLAST of MELO3C024346 vs. ExPASy TrEMBL
Match: A0A5A7UU12 (Subtilisin-like protease SBT1.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G001180 PE=3 SV=1)

HSP 1 Score: 1490.3 bits (3857), Expect = 0.0e+00
Identity = 745/748 (99.60%), Postives = 748/748 (100.00%), Query Frame = 0

Query: 23  VFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTKLLYNYERAANGFS 82
           +FSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPT+LLYNYERAANGFS
Sbjct: 3   LFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTRLLYNYERAANGFS 62

Query: 83  ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 142
           ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL
Sbjct: 63  ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 122

Query: 143 DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL 202
           DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL
Sbjct: 123 DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL 182

Query: 203 KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG 262
           KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG
Sbjct: 183 KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG 242

Query: 263 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS 322
           CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS
Sbjct: 243 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS 302

Query: 323 GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG 382
           GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG
Sbjct: 303 GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG 362

Query: 383 DCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 442
           DCGSRYCYSGSLDSSKVAG+IVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA
Sbjct: 363 DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 422

Query: 443 DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT 502
           DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT
Sbjct: 423 DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT 482

Query: 503 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 562
           AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA
Sbjct: 483 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 542

Query: 563 FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL 622
           FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL
Sbjct: 543 FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL 602

Query: 623 TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT 682
           TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT
Sbjct: 603 TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT 662

Query: 683 RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF 742
           RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF
Sbjct: 663 RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF 722

Query: 743 GSIQWSDGIHNVRSPIAVSFKTGSIASM 771
           GSIQWSDGIHNVRSPIAVSFKTGSIASM
Sbjct: 723 GSIQWSDGIHNVRSPIAVSFKTGSIASM 750

BLAST of MELO3C024346 vs. ExPASy TrEMBL
Match: A0A5D3BXR8 (Subtilisin-like protease SBT1.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001320 PE=3 SV=1)

HSP 1 Score: 1490.3 bits (3857), Expect = 0.0e+00
Identity = 745/748 (99.60%), Postives = 748/748 (100.00%), Query Frame = 0

Query: 23  VFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTKLLYNYERAANGFS 82
           +FSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPT+LLYNYERAANGFS
Sbjct: 23  LFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTRLLYNYERAANGFS 82

Query: 83  ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 142
           ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL
Sbjct: 83  ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 142

Query: 143 DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL 202
           DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL
Sbjct: 143 DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL 202

Query: 203 KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG 262
           KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG
Sbjct: 203 KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG 262

Query: 263 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS 322
           CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS
Sbjct: 263 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS 322

Query: 323 GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG 382
           GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG
Sbjct: 323 GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG 382

Query: 383 DCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 442
           DCGSRYCYSGSLDSSKVAG+IVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA
Sbjct: 383 DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 442

Query: 443 DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT 502
           DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT
Sbjct: 443 DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT 502

Query: 503 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 562
           AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA
Sbjct: 503 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 562

Query: 563 FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL 622
           FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL
Sbjct: 563 FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL 622

Query: 623 TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT 682
           TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT
Sbjct: 623 TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT 682

Query: 683 RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF 742
           RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF
Sbjct: 683 RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF 742

Query: 743 GSIQWSDGIHNVRSPIAVSFKTGSIASM 771
           GSIQWSDGIHNVRSPIAVSFKTGSIASM
Sbjct: 743 GSIQWSDGIHNVRSPIAVSFKTGSIASM 770

BLAST of MELO3C024346 vs. ExPASy TrEMBL
Match: A0A0A0K6Z1 (Subtilase family protein OS=Cucumis sativus OX=3659 GN=Csa_7G448910 PE=3 SV=1)

HSP 1 Score: 1462.2 bits (3784), Expect = 0.0e+00
Identity = 733/771 (95.07%), Postives = 750/771 (97.28%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MANFHLPSSSFFFFF+   FPL+FSRSLE QETYIVHVSKSEKPSLFSSHHHWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120
           LS SPHPTKLLYNYERAANGFSARITT QAEELRRVPGIISVIPD+IRQLHTTRTPHFLG
Sbjct: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180
           LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGL+PVPARWKGTCDTGEG SA AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180

Query: 181 NRKIIGARAFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F+GYESNLRGSLK SSDFKSARDT+GHGTHTASTAAGSFV NASFFQYAR
Sbjct: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420
           SGVSLYSGDPLGDSKL LVYGGDCGSRYCYSGSLDSSKVAG+IVVCDRGGNARVAKGGAV
Sbjct: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVG IAGNKLRDYIHTDPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540
           IGDSPPAP+VA+FSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSG PIKDL+TSEESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600

Query: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660
           FVHGAGHI+PNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSY QLCEHKL+N
Sbjct: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660

Query: 661 PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE 720
           PGNLNYPSFSVVFD+ EVVKYTRTVTNVGDETEVVY VKVEAPQGV ISVVPNKLEFNKE
Sbjct: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720

Query: 721 KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKT-GSIASM 771
           KTT SYEITFTKINGFKESASFGSIQW DGIH+VRSPIAVSFKT GSIASM
Sbjct: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771

BLAST of MELO3C024346 vs. ExPASy TrEMBL
Match: A0A6J1HMN7 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111464338 PE=3 SV=1)

HSP 1 Score: 1355.1 bits (3506), Expect = 0.0e+00
Identity = 674/772 (87.31%), Postives = 723/772 (93.65%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MAN HL SS FFF  + FV P++FSRSL+ QET+IVHVSKSEKP+LFSSHHHWHSSILES
Sbjct: 1   MANLHLCSSGFFFLILCFV-PVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLY+YE AANGFSARIT AQA ELRR+PG+ISVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180
           LAD+ GLWA+TNYADDVIIGVLDTGIWPERPSFSDEGL+PVPA WKGTC TG G SA AC
Sbjct: 121 LADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASAC 180

Query: 181 NRKIIGARAFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F GYES +R SL+GSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWE GCYDSDILAAMDQAI+DGV +ISLSVGSSGR PAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDS 300

Query: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420
           SGVSLYSGDPLGDSKLPLVY GDCG RYCYSG+LDSSKVAG+IVVCDRGGNARVAKG AV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480
           K+AGGLGM+LANTEENGEELLADSHLIPGTM+GEIAGN L+DYIH+DP+PTATIVFRGTV
Sbjct: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTV 480

Query: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540
           IGDSPPAP+VA+FSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPT L+IDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSAL+TTSYSLDSSG PI DL+TS++SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNP 600

Query: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660
           FVHGAGHIDPN+ALNPGLIYDL PQDYVSFLCSIGYDS+QIAVFVK S Y+QLCEHKL++
Sbjct: 601 FVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTS 660

Query: 661 PGNLNYPSFSVVFD-DG-EVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFN 720
           PGNLNYPSF+VVFD DG EVVKYTRTVTNVGDE + VY VKVE+PQGVEI+V+PNKLEF+
Sbjct: 661 PGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFS 720

Query: 721 KEKTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 771
            +  T SYEITFTKING K SASFGSIQWSDG H VRSP+AVSF +G IASM
Sbjct: 721 AKDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of MELO3C024346 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 978.0 bits (2527), Expect = 4.3e-285
Identity = 492/770 (63.90%), Postives = 595/770 (77.27%), Query Frame = 0

Query: 8   SSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHP 67
           SS FF F +L  F    S S +  E+YIVHV +S KPSLFSSH++WH S+L SL  SP P
Sbjct: 7   SSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQP 66

Query: 68  TKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGL 127
             LLY+Y RA +GFSAR++  Q   LRR P +ISVIPD+ R++HTT TP FLG + N GL
Sbjct: 67  ATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGL 126

Query: 128 WADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGA 187
           W+++NY +DVI+GVLDTGIWPE PSFSD GL P+P+ WKG C+ G    A +CNRK+IGA
Sbjct: 127 WSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGA 186

Query: 188 RAFFFGYESNLRGSLK-GSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGM 247
           RAF+ GY +   G+ K  + + +S RDT+GHGTHTASTAAGS V NAS +QYARG A GM
Sbjct: 187 RAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGM 246

Query: 248 ASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAF 307
           AS+ARIAAYKICW  GCYDSDILAAMDQA++DGV VISLSVG+SG AP Y+ DSIAIGAF
Sbjct: 247 ASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAF 306

Query: 308 GAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLY 367
           GA +HGIVVSCSAGNSGP P TA NIAPWI+TVGAST+DREF A+ I GD +VF+G SLY
Sbjct: 307 GATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLY 366

Query: 368 SGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAVKSAGGL 427
           +G+ L DS+L LVY GDCGSR CY G L+SS V G+IV+CDRGGNARV KG AVK AGG 
Sbjct: 367 AGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGA 426

Query: 428 GMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPP 487
           GM+LANT E+GEEL ADSHL+P TMVG  AG+++RDYI T  +PTA I F GT+IG SPP
Sbjct: 427 GMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPP 486

Query: 488 APKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTS 547
           +P+VA+FSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L+IDPRRV+FNIISGTS
Sbjct: 487 SPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTS 546

Query: 548 MSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAG 607
           MSCPHVSG+AALLRKA P WSPAAIKSAL+TT+Y +++SG PI+DLAT + SN F+HGAG
Sbjct: 547 MSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAG 606

Query: 608 HIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCE-HKLSNPGNLN 667
           H+DPN+ALNPGL+YD+  ++YV+FLC++GY+   I VF++  +    CE  KL   G+LN
Sbjct: 607 HVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLN 666

Query: 668 YPSFSVVF-DDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTL 727
           YPSFSVVF   GEVVKY R V NVG   + VY V V++P  VEI V P+KL F+KEK+ L
Sbjct: 667 YPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVL 726

Query: 728 SYEITFTKI-----NGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIAS 770
            YE+TF  +      G      FGSI+W+DG H V+SP+AV +  GS+ S
Sbjct: 727 EYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of MELO3C024346 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 767.3 bits (1980), Expect = 1.2e-221
Identity = 416/764 (54.45%), Postives = 528/764 (69.11%), Query Frame = 0

Query: 8   SSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHP 67
           SS+ FF  +   F  V S S + Q TYIVH++KS+ PS F  H +W+ S L S+S S   
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSD-QGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS--- 66

Query: 68  TKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLG- 127
            +LLY YE A +GFS R+T  +A+ L   PG+ISV+P+   +LHTTRTP FLGL ++   
Sbjct: 67  AELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD 126

Query: 128 LWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIG 187
           L+ +     DV++GVLDTG+WPE  S+SDEG  P+P+ WKG C+ G   +A  CNRK+IG
Sbjct: 127 LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIG 186

Query: 188 ARAFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGM 247
           AR F  GYES + G +  S + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGM
Sbjct: 187 ARFFARGYESTM-GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGM 246

Query: 248 ASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAF 307
           A RAR+A YK+CW  GC+ SDILAA+D+AI+D V+V+S+S+G  G    YYRD +AIGAF
Sbjct: 247 APRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAF 306

Query: 308 GAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLY 367
            AM+ GI+VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+ + F+GVSL+
Sbjct: 307 AAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLF 366

Query: 368 SGDPLGDSKLPLVYGGDCGS----RYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAVKS 427
            G+ L D  LP +Y G+  +      C +G+L   KV G+IV+CDRG NARV KG  VK+
Sbjct: 367 KGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKA 426

Query: 428 AGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIG 487
           AGG+GM+LANT  NGEEL+AD+HL+P T VGE AG+ +R Y+ TDPNPTA+I   GTV+G
Sbjct: 427 AGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG 486

Query: 488 DSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNII 547
              P+P VA+FSSRGPN  T  ILKPD+IAPGVNILA W+G + PTGL  D RRVEFNII
Sbjct: 487 -VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNII 546

Query: 548 SGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFV 607
           SGTSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D+AT + S PF 
Sbjct: 547 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFD 606

Query: 608 HGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCE-HKLSNP 667
           HGAGH+ P  A NPGLIYDLT +DY+ FLC++ Y S QI      S  +  C+  K  + 
Sbjct: 607 HGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR---SVSRRNYTCDPSKSYSV 666

Query: 668 GNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEA-PQGVEISVVPNKLEFNKE 727
            +LNYPSF+V  D     KYTRTVT+VG      Y VKV +   GV+ISV P  L F + 
Sbjct: 667 ADLNYPSFAVNVDGVGAYKYTRTVTSVGGAG--TYSVKVTSETTGVKISVEPAVLNFKEA 726

Query: 728 KTTLSYEITFTKINGFKESA--SFGSIQWSDGIHNVRSPIAVSF 763
               SY +TFT ++  K S   SFGSI+WSDG H V SP+A+S+
Sbjct: 727 NEKKSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of MELO3C024346 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 749.2 bits (1933), Expect = 3.3e-216
Identity = 399/760 (52.50%), Postives = 514/760 (67.63%), Query Frame = 0

Query: 8   SSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHP 67
           SSS       F+F L+ + +   ++TYI+ V+ S+KP  F +HH W++S L S S     
Sbjct: 7   SSSSITIITTFLFLLLHTTA---KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES----- 66

Query: 68  TKLLYNYERAANGFSARITTAQAEE-LRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLG 127
             LLY Y  + +GFSA + + +A+  L     I+ +  D +  LHTTRTP FLGL    G
Sbjct: 67  -SLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFG 126

Query: 128 LWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIG 187
           +    + ++ VIIGVLDTG+WPE  SF D  +  +P++WKG C++G    +  CN+K+IG
Sbjct: 127 VHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIG 186

Query: 188 ARAFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGM 247
           AR+F  G++    G      +  S RD DGHGTHT++TAAGS V+NASF  YA G ARGM
Sbjct: 187 ARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGM 246

Query: 248 ASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAF 307
           A+RAR+A YK+CW  GC+ SDILAAMD+AI DGVDV+SLS+G  G AP YYRD+IAIGAF
Sbjct: 247 ATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLG-GGSAP-YYRDTIAIGAF 306

Query: 308 GAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLY 367
            AM+ G+ VSCSAGNSGP   +  N+APW++TVGA T+DR+F A   LG+ +  +GVSLY
Sbjct: 307 SAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLY 366

Query: 368 SGDPLGDSKLPLVY--GGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAVKSAG 427
           SG  +G   L LVY  G    S  C  GSLDSS V G+IVVCDRG NARV KG  V+ AG
Sbjct: 367 SGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAG 426

Query: 428 GLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDS 487
           GLGM++ANT  +GEEL+ADSHL+P   VG+  G+ LR+Y+ +D  PTA +VF+GTV+ D 
Sbjct: 427 GLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DV 486

Query: 488 PPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISG 547
            P+P VA+FSSRGPN  T EILKPDVI PGVNILAGWS    PTGL+ D RR +FNI+SG
Sbjct: 487 KPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSG 546

Query: 548 TSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHG 607
           TSMSCPH+SG+A LL+ A P WSP+AIKSAL+TT+Y LD++ +P+ D A +  SNP+ HG
Sbjct: 547 TSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHG 606

Query: 608 AGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNL 667
           +GH+DP +AL+PGL+YD++ ++Y+ FLCS+ Y    I   VK  S +  C  K S+PG L
Sbjct: 607 SGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQL 666

Query: 668 NYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTL 727
           NYPSFSV+F    VV+YTR VTNVG  +  VY V V     V ISV P+KL F       
Sbjct: 667 NYPSFSVLFGGKRVVRYTREVTNVGAASS-VYKVTVNGAPSVGISVKPSKLSFKSVGEKK 726

Query: 728 SYEITFTKING--FKESASFGSIQWSDGIHNVRSPIAVSF 763
            Y +TF    G      A FGSI WS+  H VRSP+A S+
Sbjct: 727 RYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of MELO3C024346 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 729.9 bits (1883), Expect = 2.1e-210
Identity = 393/778 (50.51%), Postives = 519/778 (66.71%), Query Frame = 0

Query: 11  FFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTKL 70
           F+FFF+L +     S S     TYIVHV    KPS+F +H HW++S L SL+ S  P  +
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPSI 64

Query: 71  LYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGL--ADNLGLW 130
           ++ Y+   +GFSAR+T+  A +L   P +ISVIP+++R LHTTR+P FLGL   D  GL 
Sbjct: 65  IHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLL 124

Query: 131 ADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGAR 190
            ++++  D++IGV+DTG+WPERPSF D GL PVP +WKG C   +     ACNRK++GAR
Sbjct: 125 EESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGAR 184

Query: 191 AFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMAS 250
            F  GYE+   G +  +++F+S RD+DGHGTHTAS +AG +V  AS   YA G A GMA 
Sbjct: 185 FFCGGYEAT-NGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAP 244

Query: 251 RARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGA 310
           +AR+AAYK+CW  GCYDSDILAA D A++DGVDVISLSVG  G    YY D+IAIGAFGA
Sbjct: 245 KARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGA 304

Query: 311 MQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSG 370
           +  GI VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+ ++ SGVS+Y G
Sbjct: 305 IDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGG 364

Query: 371 DPLGDSKL-PLVYGGDC------GSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAVK 430
             L   ++ PLVYGG         S  C  GSLD + V G+IV+CDRG N+R  KG  V+
Sbjct: 365 PGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVR 424

Query: 431 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYI------HTDPNPTATIV 490
             GGLGM++AN   +GE L+AD H++P T VG   G+++R YI       +  +PTATIV
Sbjct: 425 KNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIV 484

Query: 491 FRGTVIGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPR 550
           F+GT +G   PAP VASFS+RGPN  T EILKPDVIAPG+NILA W     P+G+  D R
Sbjct: 485 FKGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNR 544

Query: 551 RVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATS 610
           R EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y++D+SG P+ D +T 
Sbjct: 545 RTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTG 604

Query: 611 EESNPFVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCE 670
             S+   +G+GH+ P +A++PGL+YD+T  DY++FLC+  Y    I    +  +    C+
Sbjct: 605 NTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD---CD 664

Query: 671 --HKLSNPGNLNYPSFSVVFDD-GE---VVKYTRTVTNVGDETEVVYGVKVEAPQGVEIS 730
              +  + GNLNYPSFSVVF   GE      + RTVTNVGD ++ VY +K+  P+G  ++
Sbjct: 665 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVT 724

Query: 731 VVPNKLEFNKEKTTLSYEI----TFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFK 764
           V P KL F +    LS+ +    T  K++    +   G I WSDG  NV SP+ V+ +
Sbjct: 725 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of MELO3C024346 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 704.9 bits (1818), Expect = 7.1e-203
Identity = 374/774 (48.32%), Postives = 509/774 (65.76%), Query Frame = 0

Query: 13  FFFVLFVFPLVF-----SRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLS----- 72
           F F++    L+F     +  + T++TY++H+ KS  P  +++H  W+SS + S++     
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 73  -PSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGL 132
               +  ++LY Y+ A +G +A++T  +AE L    G+++VIP+   +LHTTR+P FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 133 --ADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALA 192
              ++  +WA+     DV++GVLDTGIWPE  SF+D G++PVPA W+G C+TG+      
Sbjct: 131 ERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRN 190

Query: 193 CNRKIIGARAFFFGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYA 252
           CNRKI+GAR F+ GYE+   G +    ++KS RD DGHGTHTA+T AGS VK A+ F +A
Sbjct: 191 CNRKIVGARVFYRGYEA-ATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFA 250

Query: 253 RGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRD 312
            G ARGMA +AR+AAYK+CW  GC+ SDIL+A+DQA++DGV V+S+S+G  G    Y RD
Sbjct: 251 YGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLG--GGVSTYSRD 310

Query: 313 SIAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRV 372
           S++I  FGAM+ G+ VSCSAGN GP P +  N++PWI TVGAST+DR+F A V +G  R 
Sbjct: 311 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 370

Query: 373 FSGVSLYSGDPL--GDSKLPLVYGGDCGS-----RYCYSGSLDSSKVAGRIVVCDRGGNA 432
           F GVSLY G  +   + + PLVY G   S      +C  G+LD   VAG+IV+CDRG   
Sbjct: 371 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 430

Query: 433 RVAKGGAVKSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTA 492
           RV KG  VK AGG+GMVL NT  NGEEL+ADSH++P   VGE  G  ++ Y  T    TA
Sbjct: 431 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATA 490

Query: 493 TIVFRGTVIGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNI 552
           ++   GT IG   P+P VA+FSSRGPN+ + EILKPD++APGVNILA W+G  +P+ L+ 
Sbjct: 491 SLEILGTRIG-IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSS 550

Query: 553 DPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDL 612
           DPRRV+FNI+SGTSMSCPHVSGVAAL++   P WSPAAIKSAL+TT+Y  D+   P+ D 
Sbjct: 551 DPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDA 610

Query: 613 ATSEESNPFVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQ 672
           + +  S+P+ HGAGHIDP +A +PGL+YD+ PQ+Y  FLC+      Q+ VF K S  ++
Sbjct: 611 SGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHS--NR 670

Query: 673 LCEHKLS-NPGNLNYPSFSVVFDDGEVVK---YTRTVTNVGDETEVVYGVKVEAPQGVEI 732
            C+H L+ NPGNLNYP+ S +F +   VK     RTVTNVG      Y V V   +G  +
Sbjct: 671 TCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHIS-SYKVSVSPFKGASV 730

Query: 733 SVVPNKLEFNKEKTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSF 763
           +V P  L F  +   LSY +TF      K    FG + W    H VRSP+ +++
Sbjct: 731 TVQPKTLNFTSKHQKLSYTVTFRTRFRMKR-PEFGGLVWKSTTHKVRSPVIITW 776