MELO3C024317.jh1 (gene) Melon (Harukei-3) v1.41

BLAST of MELO3C024317.jh1 vs. NCBI nr
Match: XP_008462253.1 (PREDICTED: GDSL esterase/lipase 5-like isoform X1 [Cucumis melo] >KAA0059365.1 GDSL esterase/lipase 5-like isoform X1 [Cucumis melo var. makuwa] >TYK03961.1 GDSL esterase/lipase 5-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 763 bits (1971), Expect = 1.48e-278
Identity = 373/373 (100.00%), Postives = 373/373 (100.00%), Query Frame = 0

Query: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60
           MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR
Sbjct: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120
           ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG
Sbjct: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120

Query: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180
           ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240
           SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC
Sbjct: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240

Query: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC 300
           LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC
Sbjct: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC 300

Query: 301 GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY 360
           GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY
Sbjct: 301 GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY 360

Query: 361 NLKQFFQYIQSQP 373
           NLKQFFQYIQSQP
Sbjct: 361 NLKQFFQYIQSQP 373

BLAST of MELO3C024317.jh1 vs. NCBI nr
Match: XP_004141726.1 (GDSL lipase isoform X1 [Cucumis sativus] >KGN45451.1 hypothetical protein Csa_016520 [Cucumis sativus])

HSP 1 Score: 698 bits (1802), Expect = 8.88e-253
Identity = 341/374 (91.18%), Postives = 351/374 (93.85%), Query Frame = 0

Query: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60
           MKI ++HF FLIF+AVFFIAQSS IDDVSS EKRLAFFIFGDS FDPGNNNFINTTEDFR
Sbjct: 1   MKIPNLHFLFLIFTAVFFIAQSSLIDDVSSPEKRLAFFIFGDSLFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120
           ANFTPYG+SFF+TPTGRFSD RLVPDFVAEYANLPLIPAYLDP NKRYIHGVNFASGGGG
Sbjct: 61  ANFTPYGESFFKTPTGRFSDGRLVPDFVAEYANLPLIPAYLDPHNKRYIHGVNFASGGGG 120

Query: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180
           ALVETHRGFAIDIETQLRYFKKVERSIRKKLGD RAYNLFSNSVYLFSIGGNDYIVPFEG
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDWRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKK-DGHGS 240
           SP+F+KYTE EYVNMVIGN TAVLEEIYKKGGRKFAFVAVPPLGCLPHIRL+KK  GHGS
Sbjct: 181 SPIFDKYTEREYVNMVIGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLVKKAGGHGS 240

Query: 241 CLDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKAC 300
           C DE SALVRLHNKLL  ALQKLADKL GFKYT+GDTYTMLQNRIDNPSKYG KE K AC
Sbjct: 241 CWDEPSALVRLHNKLLPGALQKLADKLQGFKYTVGDTYTMLQNRIDNPSKYGFKEEKTAC 300

Query: 301 CGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINP 360
           CGSGK RGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINP
Sbjct: 301 CGSGKFRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINP 360

Query: 361 YNLKQFFQYIQSQP 373
           Y+LKQFFQY  SQP
Sbjct: 361 YSLKQFFQYASSQP 374

BLAST of MELO3C024317.jh1 vs. NCBI nr
Match: XP_038897092.1 (GDSL lipase-like [Benincasa hispida])

HSP 1 Score: 682 bits (1761), Expect = 1.51e-246
Identity = 328/367 (89.37%), Postives = 343/367 (93.46%), Query Frame = 0

Query: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60
           MKIS+ HF FLIFS++F IA+SSRIDDV  SE RLAFFIFGDSFFDPGNNNFINTTEDFR
Sbjct: 1   MKISNFHFHFLIFSSLFLIARSSRIDDVPPSENRLAFFIFGDSFFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120
           ANFTPYG+SFF+ PTGRFSD RL+PDF+AEYANLPLIPAYLDPRNK YIHGVNFASGGGG
Sbjct: 61  ANFTPYGESFFKCPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG 120

Query: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180
           ALVETHRGFAIDIETQLRYFKKVERS+RKKLGDSRAYNLF NSVY+FSIGGNDYIVPFEG
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSMRKKLGDSRAYNLFLNSVYMFSIGGNDYIVPFEG 180

Query: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240
           SPV EKYTETEYVNMVIGN TAVLEEIYKKGGRKFAF AVPPLGCLPHIRLMKK GHGSC
Sbjct: 181 SPVLEKYTETEYVNMVIGNTTAVLEEIYKKGGRKFAFAAVPPLGCLPHIRLMKKGGHGSC 240

Query: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC 300
            DEASAL RLHNKLL  ALQKLADKL GFKYT+ DTYT+LQNRIDNPSKYG KEGKKACC
Sbjct: 241 WDEASALTRLHNKLLPIALQKLADKLQGFKYTVADTYTLLQNRIDNPSKYGFKEGKKACC 300

Query: 301 GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY 360
           GSG+LRGIYSCGGMRG KEFELCENPNEYLFFDSYHPNE+AYEQFAKLMWSGD+QVI PY
Sbjct: 301 GSGELRGIYSCGGMRGQKEFELCENPNEYLFFDSYHPNEKAYEQFAKLMWSGDAQVIKPY 360

Query: 361 NLKQFFQ 367
           NLKQ FQ
Sbjct: 361 NLKQLFQ 367

BLAST of MELO3C024317.jh1 vs. NCBI nr
Match: KAA0059367.1 (GDSL esterase/lipase 5-like [Cucumis melo var. makuwa] >TYK03959.1 GDSL esterase/lipase 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 639 bits (1648), Expect = 3.23e-229
Identity = 309/374 (82.62%), Postives = 333/374 (89.04%), Query Frame = 0

Query: 1   MKISSV-HFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDF 60
           MKI S+ HF FLIFS  FFIAQ SRI +++SS+  LAFF+FGDSF DPGNNNFINTTE F
Sbjct: 7   MKIISIFHFLFLIFSTFFFIAQPSRIHNLTSSQNPLAFFVFGDSFVDPGNNNFINTTESF 66

Query: 61  RANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGG 120
           RANFTPYG++FF++PTGRFSD R+VPDF+AEYANLPLI AYLDP NK YIHG NFASGG 
Sbjct: 67  RANFTPYGQTFFKSPTGRFSDGRIVPDFIAEYANLPLIQAYLDPHNKLYIHGANFASGGA 126

Query: 121 GALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFE 180
           G LVETHRG AI IETQLRYFKKVERS+RKKLGDSRAYNLFSNSVY F +GGNDY VPFE
Sbjct: 127 GVLVETHRGLAIGIETQLRYFKKVERSMRKKLGDSRAYNLFSNSVYFFHVGGNDYKVPFE 186

Query: 181 GSPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGS 240
            S V E Y+ETE+V+MVIGN++AVLEEIYKKGGRKFAFVA+PPLGCLP+ RL+KK+G GS
Sbjct: 187 SSSVHETYSETEHVHMVIGNLSAVLEEIYKKGGRKFAFVAIPPLGCLPNTRLLKKEGDGS 246

Query: 241 CLDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKAC 300
           C DE SAL  LHNKL   ALQK ADK  GFKYT+ D YTMLQNRIDNPSKYGLKEGKKAC
Sbjct: 247 CWDEVSALAVLHNKLFPIALQKFADKFPGFKYTVADMYTMLQNRIDNPSKYGLKEGKKAC 306

Query: 301 CGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINP 360
           CGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINP
Sbjct: 307 CGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINP 366

Query: 361 YNLKQFFQYIQSQP 373
           YNLKQFFQYIQSQP
Sbjct: 367 YNLKQFFQYIQSQP 380

BLAST of MELO3C024317.jh1 vs. NCBI nr
Match: XP_022964041.1 (GDSL esterase/lipase 5-like [Cucurbita moschata])

HSP 1 Score: 630 bits (1624), Expect = 1.31e-225
Identity = 302/372 (81.18%), Postives = 330/372 (88.71%), Query Frame = 0

Query: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60
           MKIS+VHFFF IF  + F +  S+IDDV SS   +AFF+FGDSF DPGNNNFINTTEDFR
Sbjct: 5   MKISNVHFFFKIFFTILFTSHGSQIDDVPSSNNGVAFFVFGDSFLDPGNNNFINTTEDFR 64

Query: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120
           ANFTPYG++FF +PTGRFSD RL+PDF+AEYANLPLIPAYLDP N  YIHGVNFASGGGG
Sbjct: 65  ANFTPYGETFFNSPTGRFSDGRLIPDFIAEYANLPLIPAYLDPHNNLYIHGVNFASGGGG 124

Query: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180
           ALVETH+GFAI+IETQLRYFKKVERS+RKKLGD+RA +L S+SVY+FSIGGNDYIV FEG
Sbjct: 125 ALVETHQGFAINIETQLRYFKKVERSLRKKLGDARASSLLSDSVYMFSIGGNDYIVAFEG 184

Query: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240
           SPV EKYTE EYVNMVIGNVT+VL+EIYKKGGRKFAF+AVPPLGC+PH RLMK  GHGSC
Sbjct: 185 SPVLEKYTEIEYVNMVIGNVTSVLQEIYKKGGRKFAFLAVPPLGCMPHTRLMKVGGHGSC 244

Query: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC 300
            DEASAL RLHNKLL AALQKLA  L GFKYT+ DTY MLQNRIDNPSKYGLK+G+ ACC
Sbjct: 245 WDEASALARLHNKLLPAALQKLAHDLQGFKYTLADTYNMLQNRIDNPSKYGLKDGRTACC 304

Query: 301 GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY 360
           GSG+LRGIYSCGGMRG  EFELCENPNEYLFFDSYHPNERAYEQFAKL+W GD+Q INP 
Sbjct: 305 GSGELRGIYSCGGMRGQTEFELCENPNEYLFFDSYHPNERAYEQFAKLIWYGDAQFINPS 364

Query: 361 NLKQFFQYIQSQ 372
           NLKQFFQ   SQ
Sbjct: 365 NLKQFFQCGSSQ 376

BLAST of MELO3C024317.jh1 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.8e-101
Identity = 180/338 (53.25%), Postives = 230/338 (68.05%), Query Frame = 0

Query: 30  SSEKRLAFFIFGDSFFDPGNNNFINTTEDFRANFTPYGKSFFRTPTGRFSDSRLVPDFVA 89
           SS++  A FIFGDS FDPGNNN INT  +F+ANF PYG+S+F +PTGRFSD R++PDF+A
Sbjct: 27  SSQQAAALFIFGDSVFDPGNNNHINTHVNFKANFWPYGQSYFSSPTGRFSDGRIIPDFIA 86

Query: 90  EYANLPLIPAYLDPRNKRYIHGVNFASGGGGALVETHRGFAIDIETQLRYFKKVERSIRK 149
           EYA+LP+IPAYL+P N  + HG NFAS G GAL+ +H G A+ ++TQLRYF  +    R+
Sbjct: 87  EYASLPIIPAYLEPNND-FTHGANFASAGAGALIASHAGLAVGLQTQLRYFGDLVDHYRQ 146

Query: 150 KLGDSRAYNLFSNSVYLFSIGGNDYIVPFEGSPVFEKYTETEYVNMVIGNVTAVLEEIYK 209
            LGD ++  L S++VYLFS GGNDY  P      +  YT+ +YV++VIGN+T V++ IY+
Sbjct: 147 NLGDIKSRQLLSDAVYLFSCGGNDYQSP------YYPYTQEQYVDIVIGNMTNVIKGIYE 206

Query: 210 KGGRKFAFVAVPPLGCLPHIRLMKKDGHGSCLDEASALVRLHNKLLHAALQKLADKLHGF 269
           KGGRKF  V VP +GC P +R   K    +C  E   L RLHN+     L++L  +L GF
Sbjct: 207 KGGRKFGVVNVPLIGCWPGMR--AKQPGNTCNTEVDELTRLHNQAFAKRLEQLEKQLEGF 266

Query: 270 KYTIGDTYTMLQNRIDNPSKYGLKEGKKACCGSGKLRGIYSCGGMRGVKEFELCENPNEY 329
            Y   D  T + NR+ NPSKYG KEG+ ACCGSG   G Y CG    +KEF LC+N  EY
Sbjct: 267 VYAKFDLSTAILNRMKNPSKYGFKEGESACCGSGPFGGNYDCG---RIKEFGLCDNATEY 326

Query: 330 LFFDSYHPNERAYEQFAKLMWSGDSQVINPYNLKQFFQ 368
            FFD +HPNE A  QFA++ W GDS V  PYNLK  F+
Sbjct: 327 FFFDPFHPNELASRQFAEMFWDGDSMVTQPYNLKALFE 352

BLAST of MELO3C024317.jh1 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 359.8 bits (922), Expect = 3.8e-98
Identity = 179/372 (48.12%), Postives = 250/372 (67.20%), Query Frame = 0

Query: 5   SVHFFFLIFSAVFFIAQSSRIDDVSSSEKRL-AFFIFGDSFFDPGNNNFINTTEDFRANF 64
           S   FF++ S + F+A  S      + +  + A F+FGDSF D GNNN+INTT   +ANF
Sbjct: 16  SSFIFFIVSSTILFLAGKSSAKISHNGDNNVTALFLFGDSFLDAGNNNYINTTTLDQANF 75

Query: 65  TPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRN-KRYIHGVNFASGGGGAL 124
            PYG++FF  PTGRFSD RL+ DF+AEYANLPLIP +L+P N ++ ++GVNFAS G GAL
Sbjct: 76  PPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASAGAGAL 135

Query: 125 VETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPF---E 184
           VET +G  I++ TQL ++KKVER  R   G   +    S +VYL SIG NDY   F   +
Sbjct: 136 VETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSSIFLTNQ 195

Query: 185 GSPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGS 244
             P+    + +++V++VIGN+T  + EIYK GGRKF F+ VP LGC P +R+++     S
Sbjct: 196 SLPI----SMSQHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRILQPKNDDS 255

Query: 245 CLDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKAC 304
           CL +AS L  +HN+ L   L ++  ++ GFK+++ D    L+ R+ +PSK+G KEG++AC
Sbjct: 256 CLRDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGFKEGEEAC 315

Query: 305 CGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSG----DSQ 364
           CG+GK RG++SCGG R VKE++LCENP +Y+F+DS H  +  Y QFA L+W+G    DS 
Sbjct: 316 CGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNGGHMSDSL 375

Query: 365 VINPYNLKQFFQ 368
           V+ PYN+   FQ
Sbjct: 376 VVGPYNINNLFQ 383

BLAST of MELO3C024317.jh1 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 8.0e-96
Identity = 174/366 (47.54%), Postives = 238/366 (65.03%), Query Frame = 0

Query: 6   VHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFRANFTP 65
           V   FL ++ +  I   + ID+ +    + A F+FGDS FD GNNN+I+T    R+N+ P
Sbjct: 7   VSITFLAYTIIISIGSINCIDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSVRSNYWP 66

Query: 66  YGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDP--RNKRYIHGVNFASGGGGALV 125
           YG++ F++PTGR SD RL+PDF+AEYA LPLIP  L P   N ++ +GVNFASGG GALV
Sbjct: 67  YGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASGGAGALV 126

Query: 126 ETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPF-EGSP 185
            T  G  I++ TQL  FKKVE  +R KLGD+    + S +VYLF IG NDY  PF   S 
Sbjct: 127 GTFSGLVINLRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSS 186

Query: 186 VFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSCLD 245
           +F+  +  +YV+ V+GN+T V +E+Y  GGRKF  +   P  C P   ++ +    SC  
Sbjct: 187 LFQSISNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQ 246

Query: 246 EASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACCGS 305
             + L+ +HN+ L   L++L  +L GFKY + D +T L  R+++PSKYG KEGKKACCGS
Sbjct: 247 PVTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKEGKKACCGS 306

Query: 306 GKLRGIYSCGGMRGVKE-FELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPYN 365
           G LRGI +CGG  G+ + +ELCEN  +YLFFD +H  E+A  Q A+L+WSG + +  PYN
Sbjct: 307 GPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGPTNITGPYN 366

Query: 366 LKQFFQ 368
           LK  F+
Sbjct: 367 LKALFE 372

BLAST of MELO3C024317.jh1 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 2.6e-94
Identity = 171/369 (46.34%), Postives = 244/369 (66.12%), Query Frame = 0

Query: 5   SVHFFFLIFSAVFFIAQSSRIDDVSSS--EKRLAFFIFGDSFFDPGNNNFINTTEDFRAN 64
           S    F  ++ +  I   +  D+ +++    + A F+FGDS FD GNNN+I+T   FR+N
Sbjct: 6   STLIIFFAYTTIILIGSINCRDNNNNNLVTNQSALFVFGDSVFDAGNNNYIDTLPSFRSN 65

Query: 65  FTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRN--KRYIHGVNFASGGGG 124
           + PYG++ F+ PTGR SD R +PDF+AEYA LPLIPAYL P N   ++ +GV+FAS G G
Sbjct: 66  YWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLIPAYLQPSNGKNQFPYGVSFASAGAG 125

Query: 125 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFE- 184
           ALV T  G  I++++QL  FKKVE+ +R  LG+++   + S +VYLF IG NDY  PF  
Sbjct: 126 ALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEAQGKMVISRAVYLFHIGVNDYQYPFST 185

Query: 185 GSPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGS 244
            S +F+   +  YV+ V+GN TAV++E+YK GGRKF F+ +    C P   ++ +   G+
Sbjct: 186 NSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGRKFGFLNMGAYDCAPASLIIDQTKIGT 245

Query: 245 CLDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKAC 304
           C    + L+ LHN+ L + L++L  +L GFKY + D +T L  R++NPSKYG KEGK AC
Sbjct: 246 CFKPVTELINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYGFKEGKMAC 305

Query: 305 CGSGKLRGIYSCGGMRGVKE-FELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVIN 364
           CG+G LRGI +CGG  GV + +ELCE   +YLFFD +H  E+A++Q A+L+WSG + V  
Sbjct: 306 CGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFFDHFHLTEKAHQQIAELIWSGPTNVTK 365

Query: 365 PYNLKQFFQ 368
           PYNL+  F+
Sbjct: 366 PYNLQALFE 374

BLAST of MELO3C024317.jh1 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 328.9 bits (842), Expect = 7.2e-89
Identity = 167/366 (45.63%), Postives = 229/366 (62.57%), Query Frame = 0

Query: 6   VHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFRANFTP 65
           V   F +++ +  I   + ID+ +    + A F+FGDS FD GNNN+INT   FR+N  P
Sbjct: 5   VLIIFFVYTIILSIGSINCIDNNNLVTNQAALFVFGDSLFDAGNNNYINTVSSFRSNIWP 64

Query: 66  YGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPR--NKRYIHGVNFASGGGGALV 125
           YG++ F+ PTGR SD         E A LP IP  L P   N ++ +GV+FAS G GAL 
Sbjct: 65  YGQTNFKFPTGRLSDG-------PEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGAGALA 124

Query: 126 ETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFE-GSP 185
           E+  G  I++ TQL  FK VE+S+R +LGD+    +FS +VYLF IG NDY  PF   S 
Sbjct: 125 ESFLGMVINLGTQLNNFKDVEKSLRSELGDAETKRVFSRAVYLFHIGANDYFYPFSANSS 184

Query: 186 VFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSCLD 245
            F+  ++ ++V+ VIGN+T V+EE+YK GGRKF F+ V P  C P+  +  +   GSC  
Sbjct: 185 TFKSNSKEKFVDFVIGNITFVIEEVYKMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCFK 244

Query: 246 EASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACCGS 305
             + L+ +HNK     L++L  +L GF+Y + D +T L  RI++PSKYG KEGKKACCGS
Sbjct: 245 PVAELIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYGFKEGKKACCGS 304

Query: 306 GKLRGIYSCGGMRGVKE-FELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPYN 365
           G LRGI +CG   G  + + LCEN  +YLF+DS H  E+A+ Q A+L+W+G   V  PYN
Sbjct: 305 GPLRGINTCGNRIGPSQGYGLCENVTDYLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYN 363

Query: 366 LKQFFQ 368
           LK  F+
Sbjct: 365 LKALFE 363

BLAST of MELO3C024317.jh1 vs. ExPASy TrEMBL
Match: A0A5A7V0K2 (GDSL esterase/lipase 5-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001580 PE=3 SV=1)

HSP 1 Score: 763 bits (1971), Expect = 7.19e-279
Identity = 373/373 (100.00%), Postives = 373/373 (100.00%), Query Frame = 0

Query: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60
           MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR
Sbjct: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120
           ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG
Sbjct: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120

Query: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180
           ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240
           SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC
Sbjct: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240

Query: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC 300
           LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC
Sbjct: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC 300

Query: 301 GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY 360
           GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY
Sbjct: 301 GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY 360

Query: 361 NLKQFFQYIQSQP 373
           NLKQFFQYIQSQP
Sbjct: 361 NLKQFFQYIQSQP 373

BLAST of MELO3C024317.jh1 vs. ExPASy TrEMBL
Match: A0A1S3CI17 (GDSL esterase/lipase 5-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500655 PE=3 SV=1)

HSP 1 Score: 763 bits (1971), Expect = 7.19e-279
Identity = 373/373 (100.00%), Postives = 373/373 (100.00%), Query Frame = 0

Query: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60
           MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR
Sbjct: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120
           ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG
Sbjct: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120

Query: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180
           ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240
           SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC
Sbjct: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240

Query: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC 300
           LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC
Sbjct: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC 300

Query: 301 GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY 360
           GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY
Sbjct: 301 GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY 360

Query: 361 NLKQFFQYIQSQP 373
           NLKQFFQYIQSQP
Sbjct: 361 NLKQFFQYIQSQP 373

BLAST of MELO3C024317.jh1 vs. ExPASy TrEMBL
Match: A0A0A0KCD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G448130 PE=3 SV=1)

HSP 1 Score: 698 bits (1802), Expect = 4.30e-253
Identity = 341/374 (91.18%), Postives = 351/374 (93.85%), Query Frame = 0

Query: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60
           MKI ++HF FLIF+AVFFIAQSS IDDVSS EKRLAFFIFGDS FDPGNNNFINTTEDFR
Sbjct: 1   MKIPNLHFLFLIFTAVFFIAQSSLIDDVSSPEKRLAFFIFGDSLFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120
           ANFTPYG+SFF+TPTGRFSD RLVPDFVAEYANLPLIPAYLDP NKRYIHGVNFASGGGG
Sbjct: 61  ANFTPYGESFFKTPTGRFSDGRLVPDFVAEYANLPLIPAYLDPHNKRYIHGVNFASGGGG 120

Query: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180
           ALVETHRGFAIDIETQLRYFKKVERSIRKKLGD RAYNLFSNSVYLFSIGGNDYIVPFEG
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDWRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKK-DGHGS 240
           SP+F+KYTE EYVNMVIGN TAVLEEIYKKGGRKFAFVAVPPLGCLPHIRL+KK  GHGS
Sbjct: 181 SPIFDKYTEREYVNMVIGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLVKKAGGHGS 240

Query: 241 CLDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKAC 300
           C DE SALVRLHNKLL  ALQKLADKL GFKYT+GDTYTMLQNRIDNPSKYG KE K AC
Sbjct: 241 CWDEPSALVRLHNKLLPGALQKLADKLQGFKYTVGDTYTMLQNRIDNPSKYGFKEEKTAC 300

Query: 301 CGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINP 360
           CGSGK RGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINP
Sbjct: 301 CGSGKFRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINP 360

Query: 361 YNLKQFFQYIQSQP 373
           Y+LKQFFQY  SQP
Sbjct: 361 YSLKQFFQYASSQP 374

BLAST of MELO3C024317.jh1 vs. ExPASy TrEMBL
Match: A0A5A7UXK8 (GDSL esterase/lipase 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001560 PE=3 SV=1)

HSP 1 Score: 639 bits (1648), Expect = 1.56e-229
Identity = 309/374 (82.62%), Postives = 333/374 (89.04%), Query Frame = 0

Query: 1   MKISSV-HFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDF 60
           MKI S+ HF FLIFS  FFIAQ SRI +++SS+  LAFF+FGDSF DPGNNNFINTTE F
Sbjct: 7   MKIISIFHFLFLIFSTFFFIAQPSRIHNLTSSQNPLAFFVFGDSFVDPGNNNFINTTESF 66

Query: 61  RANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGG 120
           RANFTPYG++FF++PTGRFSD R+VPDF+AEYANLPLI AYLDP NK YIHG NFASGG 
Sbjct: 67  RANFTPYGQTFFKSPTGRFSDGRIVPDFIAEYANLPLIQAYLDPHNKLYIHGANFASGGA 126

Query: 121 GALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFE 180
           G LVETHRG AI IETQLRYFKKVERS+RKKLGDSRAYNLFSNSVY F +GGNDY VPFE
Sbjct: 127 GVLVETHRGLAIGIETQLRYFKKVERSMRKKLGDSRAYNLFSNSVYFFHVGGNDYKVPFE 186

Query: 181 GSPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGS 240
            S V E Y+ETE+V+MVIGN++AVLEEIYKKGGRKFAFVA+PPLGCLP+ RL+KK+G GS
Sbjct: 187 SSSVHETYSETEHVHMVIGNLSAVLEEIYKKGGRKFAFVAIPPLGCLPNTRLLKKEGDGS 246

Query: 241 CLDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKAC 300
           C DE SAL  LHNKL   ALQK ADK  GFKYT+ D YTMLQNRIDNPSKYGLKEGKKAC
Sbjct: 247 CWDEVSALAVLHNKLFPIALQKFADKFPGFKYTVADMYTMLQNRIDNPSKYGLKEGKKAC 306

Query: 301 CGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINP 360
           CGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINP
Sbjct: 307 CGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINP 366

Query: 361 YNLKQFFQYIQSQP 373
           YNLKQFFQYIQSQP
Sbjct: 367 YNLKQFFQYIQSQP 380

BLAST of MELO3C024317.jh1 vs. ExPASy TrEMBL
Match: A0A6J1HHR5 (GDSL esterase/lipase 5-like OS=Cucurbita moschata OX=3662 GN=LOC111464186 PE=3 SV=1)

HSP 1 Score: 630 bits (1624), Expect = 6.32e-226
Identity = 302/372 (81.18%), Postives = 330/372 (88.71%), Query Frame = 0

Query: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60
           MKIS+VHFFF IF  + F +  S+IDDV SS   +AFF+FGDSF DPGNNNFINTTEDFR
Sbjct: 5   MKISNVHFFFKIFFTILFTSHGSQIDDVPSSNNGVAFFVFGDSFLDPGNNNFINTTEDFR 64

Query: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120
           ANFTPYG++FF +PTGRFSD RL+PDF+AEYANLPLIPAYLDP N  YIHGVNFASGGGG
Sbjct: 65  ANFTPYGETFFNSPTGRFSDGRLIPDFIAEYANLPLIPAYLDPHNNLYIHGVNFASGGGG 124

Query: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180
           ALVETH+GFAI+IETQLRYFKKVERS+RKKLGD+RA +L S+SVY+FSIGGNDYIV FEG
Sbjct: 125 ALVETHQGFAINIETQLRYFKKVERSLRKKLGDARASSLLSDSVYMFSIGGNDYIVAFEG 184

Query: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240
           SPV EKYTE EYVNMVIGNVT+VL+EIYKKGGRKFAF+AVPPLGC+PH RLMK  GHGSC
Sbjct: 185 SPVLEKYTEIEYVNMVIGNVTSVLQEIYKKGGRKFAFLAVPPLGCMPHTRLMKVGGHGSC 244

Query: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC 300
            DEASAL RLHNKLL AALQKLA  L GFKYT+ DTY MLQNRIDNPSKYGLK+G+ ACC
Sbjct: 245 WDEASALARLHNKLLPAALQKLAHDLQGFKYTLADTYNMLQNRIDNPSKYGLKDGRTACC 304

Query: 301 GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY 360
           GSG+LRGIYSCGGMRG  EFELCENPNEYLFFDSYHPNERAYEQFAKL+W GD+Q INP 
Sbjct: 305 GSGELRGIYSCGGMRGQTEFELCENPNEYLFFDSYHPNERAYEQFAKLIWYGDAQFINPS 364

Query: 361 NLKQFFQYIQSQ 372
           NLKQFFQ   SQ
Sbjct: 365 NLKQFFQCGSSQ 376

BLAST of MELO3C024317.jh1 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 359.8 bits (922), Expect = 2.7e-99
Identity = 179/372 (48.12%), Postives = 250/372 (67.20%), Query Frame = 0

Query: 5   SVHFFFLIFSAVFFIAQSSRIDDVSSSEKRL-AFFIFGDSFFDPGNNNFINTTEDFRANF 64
           S   FF++ S + F+A  S      + +  + A F+FGDSF D GNNN+INTT   +ANF
Sbjct: 16  SSFIFFIVSSTILFLAGKSSAKISHNGDNNVTALFLFGDSFLDAGNNNYINTTTLDQANF 75

Query: 65  TPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRN-KRYIHGVNFASGGGGAL 124
            PYG++FF  PTGRFSD RL+ DF+AEYANLPLIP +L+P N ++ ++GVNFAS G GAL
Sbjct: 76  PPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASAGAGAL 135

Query: 125 VETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPF---E 184
           VET +G  I++ TQL ++KKVER  R   G   +    S +VYL SIG NDY   F   +
Sbjct: 136 VETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSSIFLTNQ 195

Query: 185 GSPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGS 244
             P+    + +++V++VIGN+T  + EIYK GGRKF F+ VP LGC P +R+++     S
Sbjct: 196 SLPI----SMSQHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRILQPKNDDS 255

Query: 245 CLDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKAC 304
           CL +AS L  +HN+ L   L ++  ++ GFK+++ D    L+ R+ +PSK+G KEG++AC
Sbjct: 256 CLRDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGFKEGEEAC 315

Query: 305 CGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSG----DSQ 364
           CG+GK RG++SCGG R VKE++LCENP +Y+F+DS H  +  Y QFA L+W+G    DS 
Sbjct: 316 CGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNGGHMSDSL 375

Query: 365 VINPYNLKQFFQ 368
           V+ PYN+   FQ
Sbjct: 376 VVGPYNINNLFQ 383

BLAST of MELO3C024317.jh1 vs. TAIR 10
Match: AT5G40990.1 (GDSL lipase 1 )

HSP 1 Score: 352.1 bits (902), Expect = 5.7e-97
Identity = 174/366 (47.54%), Postives = 238/366 (65.03%), Query Frame = 0

Query: 6   VHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFRANFTP 65
           V   FL ++ +  I   + ID+ +    + A F+FGDS FD GNNN+I+T    R+N+ P
Sbjct: 7   VSITFLAYTIIISIGSINCIDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSVRSNYWP 66

Query: 66  YGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDP--RNKRYIHGVNFASGGGGALV 125
           YG++ F++PTGR SD RL+PDF+AEYA LPLIP  L P   N ++ +GVNFASGG GALV
Sbjct: 67  YGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASGGAGALV 126

Query: 126 ETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPF-EGSP 185
            T  G  I++ TQL  FKKVE  +R KLGD+    + S +VYLF IG NDY  PF   S 
Sbjct: 127 GTFSGLVINLRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSS 186

Query: 186 VFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSCLD 245
           +F+  +  +YV+ V+GN+T V +E+Y  GGRKF  +   P  C P   ++ +    SC  
Sbjct: 187 LFQSISNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQ 246

Query: 246 EASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACCGS 305
             + L+ +HN+ L   L++L  +L GFKY + D +T L  R+++PSKYG KEGKKACCGS
Sbjct: 247 PVTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKEGKKACCGS 306

Query: 306 GKLRGIYSCGGMRGVKE-FELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPYN 365
           G LRGI +CGG  G+ + +ELCEN  +YLFFD +H  E+A  Q A+L+WSG + +  PYN
Sbjct: 307 GPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGPTNITGPYN 366

Query: 366 LKQFFQ 368
           LK  F+
Sbjct: 367 LKALFE 372

BLAST of MELO3C024317.jh1 vs. TAIR 10
Match: AT1G53940.1 (GDSL-motif lipase 2 )

HSP 1 Score: 334.0 bits (855), Expect = 1.6e-91
Identity = 165/354 (46.61%), Postives = 235/354 (66.38%), Query Frame = 0

Query: 5   SVHFFFLIFSAVFFIAQSSRIDDVSSS--EKRLAFFIFGDSFFDPGNNNFINTTEDFRAN 64
           S    F  ++ +  I   +  D+ +++    + A F+FGDS FD GNNN+I+T   FR+N
Sbjct: 6   STLIIFFAYTTIILIGSINCRDNNNNNLVTNQSALFVFGDSVFDAGNNNYIDTLPSFRSN 65

Query: 65  FTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRN--KRYIHGVNFASGGGG 124
           + PYG++ F+ PTGR SD R +PDF+AEYA LPLIPAYL P N   ++ +GV+FAS G G
Sbjct: 66  YWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLIPAYLQPSNGKNQFPYGVSFASAGAG 125

Query: 125 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFE- 184
           ALV T  G  I++++QL  FKKVE+ +R  LG+++   + S +VYLF IG NDY  PF  
Sbjct: 126 ALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEAQGKMVISRAVYLFHIGVNDYQYPFST 185

Query: 185 GSPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGS 244
            S +F+   +  YV+ V+GN TAV++E+YK GGRKF F+ +    C P   ++ +   G+
Sbjct: 186 NSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGRKFGFLNMGAYDCAPASLIIDQTKIGT 245

Query: 245 CLDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKAC 304
           C    + L+ LHN+ L + L++L  +L GFKY + D +T L  R++NPSKYG KEGK AC
Sbjct: 246 CFKPVTELINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYGFKEGKMAC 305

Query: 305 CGSGKLRGIYSCGGMRGVKE-FELCENPNEYLFFDSYHPNERAYEQFAKLMWSG 353
           CG+G LRGI +CGG  GV + +ELCE   +YLFFD +H  E+A++Q A+L+WSG
Sbjct: 306 CGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFFDHFHLTEKAHQQIAELIWSG 359

BLAST of MELO3C024317.jh1 vs. TAIR 10
Match: AT1G53990.1 (GDSL-motif lipase 3 )

HSP 1 Score: 328.9 bits (842), Expect = 5.1e-90
Identity = 167/366 (45.63%), Postives = 229/366 (62.57%), Query Frame = 0

Query: 6   VHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFRANFTP 65
           V   F +++ +  I   + ID+ +    + A F+FGDS FD GNNN+INT   FR+N  P
Sbjct: 5   VLIIFFVYTIILSIGSINCIDNNNLVTNQAALFVFGDSLFDAGNNNYINTVSSFRSNIWP 64

Query: 66  YGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPR--NKRYIHGVNFASGGGGALV 125
           YG++ F+ PTGR SD         E A LP IP  L P   N ++ +GV+FAS G GAL 
Sbjct: 65  YGQTNFKFPTGRLSDG-------PEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGAGALA 124

Query: 126 ETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFE-GSP 185
           E+  G  I++ TQL  FK VE+S+R +LGD+    +FS +VYLF IG NDY  PF   S 
Sbjct: 125 ESFLGMVINLGTQLNNFKDVEKSLRSELGDAETKRVFSRAVYLFHIGANDYFYPFSANSS 184

Query: 186 VFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSCLD 245
            F+  ++ ++V+ VIGN+T V+EE+YK GGRKF F+ V P  C P+  +  +   GSC  
Sbjct: 185 TFKSNSKEKFVDFVIGNITFVIEEVYKMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCFK 244

Query: 246 EASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACCGS 305
             + L+ +HNK     L++L  +L GF+Y + D +T L  RI++PSKYG KEGKKACCGS
Sbjct: 245 PVAELIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYGFKEGKKACCGS 304

Query: 306 GKLRGIYSCGGMRGVKE-FELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPYN 365
           G LRGI +CG   G  + + LCEN  +YLF+DS H  E+A+ Q A+L+W+G   V  PYN
Sbjct: 305 GPLRGINTCGNRIGPSQGYGLCENVTDYLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYN 363

Query: 366 LKQFFQ 368
           LK  F+
Sbjct: 365 LKALFE 363

BLAST of MELO3C024317.jh1 vs. TAIR 10
Match: AT3G14225.1 (GDSL-motif lipase 4 )

HSP 1 Score: 320.9 bits (821), Expect = 1.4e-87
Identity = 163/374 (43.58%), Postives = 230/374 (61.50%), Query Frame = 0

Query: 2   KISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFRA 61
           + +S+     I +    I   S  +D+ +++   A F FGDS F+ GNNN+ ++   FR+
Sbjct: 5   RFNSIIIILFICTISLSIVSISCKEDLKTNQ--AALFAFGDSLFEAGNNNYFDSISSFRS 64

Query: 62  NFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDP--RNKRYIHGVNFASGGG 121
           NF PYGK+ F+ PTGR SD R++ DF+AEYA LPLIP  L P   N +  +G+NFA+   
Sbjct: 65  NFWPYGKTTFKFPTGRVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATTAA 124

Query: 122 GALVETHRG----FAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYI 181
           G    T  G     + D+ TQL  FK VE+++R  LGD+ A  + S +VYLF IG NDY 
Sbjct: 125 GVFAGTFPGSVTNLSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGANDYQ 184

Query: 182 VP-FEGSPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKK 241
            P F  +  F   T+  +++ VIGN T V+EE+YK G RKF F+++ P GC P   ++  
Sbjct: 185 YPFFANTSTFSNTTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTPSALIINS 244

Query: 242 DGHGSCLDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKE 301
              GSC +  + L+ LHN+     L++L  +L GFKY + D +T L  RI+NPS+YG KE
Sbjct: 245 TKIGSCFEPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRYGFKE 304

Query: 302 GKKACCGSGKLRGIYSCGGMRGVKE-FELCENPNEYLFFDSYHPNERAYEQFAKLMWSGD 361
           G+ ACCGSG LRGI +CG   G  + ++LCEN ++Y+FFD  H  E A++Q A+L+WSG 
Sbjct: 305 GEMACCGSGPLRGINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELIWSGP 364

Query: 362 SQVINPYNLKQFFQ 368
             V  PYNLK  F+
Sbjct: 365 PNVTAPYNLKTLFR 376