MELO3C023253 (gene) Melon (DHL92) v4

Overview
NameMELO3C023253
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionPectinesterase
Locationchr11: 548267 .. 550764 (+)
RNA-Seq ExpressionMELO3C023253
SyntenyMELO3C023253
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGACAATACCAAAGAGAGAGAGAGAGAGAGAAAATAATTTACACCGTTGTCATAAATGGTTATGAAACTGCATCATTTTGTCACCACTCCCATTTCCTCCGCCGCCGCACCAACACGTGACCCTCTCTCTCTCTTTCTATTTTCTCCTGAAATTCACTCTTTATATTTCCTCCATGAATTTCCCACCTAAATCTCAAACCCAACAATGCCTTCCCTTCTCCTTCTCTTCTTCCTCCCTCTACATTTCTCCTCCGCCGCCCACCAAACCCTTCCCCCTGAGATCGCCGCCGCCTGCAACTCCACCCGCTTCCCGGACCAATGCGCATCCTCCATCTCCCAATCCAACCGTCTCCTCCAGAATCCCACTCCGCTGCAGATCATTCATGCCGCCCTCTACCTCTCCTCAGAGAATCTCAAAACTGCTAAATCCATGGTGAAGGCCATTCTCGATTCTGCCGAGAAGATTCCCAACCGCAACCGCACCGCGGCGGCGCTAAGCTGTATGGACGCGCTGGGTTATTCGGAGTACAGAATTTCTCTTTCCAACGACGCAATTCCGCTCGGTCGAATTAAAGACGCGCGCGCGTGGTTAAGCGCGTCGCTCCTCTACCAGACCGGTTGCCGGTCGGGGTTGAAGTACGTGAACGACACGCACGAAGTGGATCAAACGATGTCGTTTCTTGACAACTTAACCGTGCTGTCGAGCAACGCGCTGAGCATGGTTGTGGCGTACGATAATTTCGGGAATGAGACGGCGGCCTGGTTACCTCCGAGGACGGAGCGAGACGGGTTCTGGGAACACAGCAGTGGAACGGGGGAGGTGGTGAGAGGGGTGAGGATTCCGGCGAAGACGGAGGTGAATGTGACGGTGTGCAAGGATGGGAGTGAGGGGTGTTATCGAACGGTACAAGCAGCGGTGGAGGCGGCGCCGGGAGAGTCGGCGGTGAGGTTTGTGATACACATAAAGGAAGGGGTTTACGAAGAGAGTGTGAGAGTGGGGATAGAGAAGAAGAATTTGGTATTTTTGGGGGATGGGATGGGCAAAACGGTCATTACTGGATCTTTGAGTGTGGGTCAGCCTGGGATCTCGACTTACAATTCGGCCACCGTCGGTGAGTCACCTTTTTTAGTTCACCAAAGCTCAGTTATTAATTATTATCAATACCTTTCTACGCAATATAAAGTTGTCGGGAGTGTACTATAAGCGTGTCGTGTCGGCTTCATTTGTGTTTGCTACATTCATGAACAAAAGGAGAATTATTTATCATTAGAGAGAATAATAATGTGTAAGAAATTGCAGGAGTTCTTGGGGATGGATTCATTGCAAGTGAACTCACCATTCAAAACACAGCAGGTCCTGATGTCCACCAAGCTGTTGCCTTTAGATCAGACAGTGACCTCTCGATCATACATAACTGTGAGTTTATAAGCAATCAAGACACTCTCTACGCCCATTCCCTCCGTCAATTCTACAACTCCTGTCGAATTTTAGGCAATGTGGACTTCATTTTTGGCAACTCAGCCTCCTTCTTTCTAAACTGTCATATCTTAGTTCGTCCTCACCCACTCAATCCTCAACTGGGAGATGAGAATCCAATCACTGCACATGGGAGGACAGACCCAGCGCAGTCGACTGGGTTTGTGTTCCAAAACTGCTTGTTAAATGGCACTGAGGAGTTCATGGCATTGTACTATGCCAATCCTCAGAGGCATAGGACCTTCTTGGGGCGGCCTTGGAAAGAGTATTCGAGAACGGTTTTTATACATTGTGAACTTGAAGCTCTTGTTTCGTCATCGGGATGGATGCCATGGAGTGGGGATATTGGATTGAATACATTATATTATGGGGAGTTTGAGAATTTTGGAACTGGTTCAAATTCGTCAAAACGAGTTGCATGGAGTAATCAAATCCCTGCAGAGCATGCTTTTAGTTACTCTATACAAAATTTCATCCAAGGAAACGAGTGGATTCCTTCCTCTTAAATGGAAAAAGAAGAAAACTAGGATTAGCATTTCAACTTTTGTTACTATACTTTACATAATTTTATGATTAAATAACAAGAAAACACTTTGTTTCTACTATTTTTCTACTTTGGGAAGTCTATTTATTTCAACTCTACAGTTCAAATGAAAATTTACTAATCTGAACCTATTAACTCTTACCACATAAAAACATGATAGAAGGAGCAAATTTATAGTTGAAGTAAACTGATTATTTGGATGCATGATTGTAATCTGCAAAGCGTAGTCAAGAAGGTAGGAGAGAATTTGTTGAGCCTGAGAGTGGTTTGCAGCTGTCTTTCCATCATTTGGTCCAATTTTTTAGTCATCTCATGTTGTGTCATGTCTTTAGAAGACACTTGGTATGTTATATGCTTGACAACAAATCCAAAGGTGTTGAAACAAAATATTATTTAATATCTCACTTTAGTTTGGTTTTGTTTGTCCCACTAAGTTAGACTTAATAAGCCTCATGTTGAAGTTCATACCTTTGTATT

mRNA sequence

AGACAATACCAAAGAGAGAGAGAGAGAGAGAAAATAATTTACACCGTTGTCATAAATGGTTATGAAACTGCATCATTTTGTCACCACTCCCATTTCCTCCGCCGCCGCACCAACACGTGACCCTCTCTCTCTCTTTCTATTTTCTCCTGAAATTCACTCTTTATATTTCCTCCATGAATTTCCCACCTAAATCTCAAACCCAACAATGCCTTCCCTTCTCCTTCTCTTCTTCCTCCCTCTACATTTCTCCTCCGCCGCCCACCAAACCCTTCCCCCTGAGATCGCCGCCGCCTGCAACTCCACCCGCTTCCCGGACCAATGCGCATCCTCCATCTCCCAATCCAACCGTCTCCTCCAGAATCCCACTCCGCTGCAGATCATTCATGCCGCCCTCTACCTCTCCTCAGAGAATCTCAAAACTGCTAAATCCATGGTGAAGGCCATTCTCGATTCTGCCGAGAAGATTCCCAACCGCAACCGCACCGCGGCGGCGCTAAGCTGTATGGACGCGCTGGGTTATTCGGAGTACAGAATTTCTCTTTCCAACGACGCAATTCCGCTCGGTCGAATTAAAGACGCGCGCGCGTGGTTAAGCGCGTCGCTCCTCTACCAGACCGGTTGCCGGTCGGGGTTGAAGTACGTGAACGACACGCACGAAGTGGATCAAACGATGTCGTTTCTTGACAACTTAACCGTGCTGTCGAGCAACGCGCTGAGCATGGTTGTGGCGTACGATAATTTCGGGAATGAGACGGCGGCCTGGTTACCTCCGAGGACGGAGCGAGACGGGTTCTGGGAACACAGCAGTGGAACGGGGGAGGTGGTGAGAGGGGTGAGGATTCCGGCGAAGACGGAGGTGAATGTGACGGTGTGCAAGGATGGGAGTGAGGGGTGTTATCGAACGGTACAAGCAGCGGTGGAGGCGGCGCCGGGAGAGTCGGCGGTGAGGTTTGTGATACACATAAAGGAAGGGGTTTACGAAGAGAGTGTGAGAGTGGGGATAGAGAAGAAGAATTTGGTATTTTTGGGGGATGGGATGGGCAAAACGGTCATTACTGGATCTTTGAGTGTGGGTCAGCCTGGGATCTCGACTTACAATTCGGCCACCGTCGGAGTTCTTGGGGATGGATTCATTGCAAGTGAACTCACCATTCAAAACACAGCAGGTCCTGATGTCCACCAAGCTGTTGCCTTTAGATCAGACAGTGACCTCTCGATCATACATAACTGTGAGTTTATAAGCAATCAAGACACTCTCTACGCCCATTCCCTCCGTCAATTCTACAACTCCTGTCGAATTTTAGGCAATGTGGACTTCATTTTTGGCAACTCAGCCTCCTTCTTTCTAAACTGTCATATCTTAGTTCGTCCTCACCCACTCAATCCTCAACTGGGAGATGAGAATCCAATCACTGCACATGGGAGGACAGACCCAGCGCAGTCGACTGGGTTTGTGTTCCAAAACTGCTTGTTAAATGGCACTGAGGAGTTCATGGCATTGTACTATGCCAATCCTCAGAGGCATAGGACCTTCTTGGGGCGGCCTTGGAAAGAGTATTCGAGAACGGTTTTTATACATTGTGAACTTGAAGCTCTTGTTTCGTCATCGGGATGGATGCCATGGAGTGGGGATATTGGATTGAATACATTATATTATGGGGAGTTTGAGAATTTTGGAACTGGTTCAAATTCGTCAAAACGAGTTGCATGGAGTAATCAAATCCCTGCAGAGCATGCTTTTAGTTACTCTATACAAAATTTCATCCAAGGAAACGAGTGGATTCCTTCCTCTTAAATGGAAAAAGAAGAAAACTAGGATTAGCATTTCAACTTTTGTTACTATACTTTACATAATTTTATGATTAAATAACAAGAAAACACTTTGTTTCTACTATTTTTCTACTTTGGGAAGTCTATTTATTTCAACTCTACAGTTCAAATGAAAATTTACTAATCTGAACCTATTAACTCTTACCACATAAAAACATGATAGAAGGAGCAAATTTATAGTTGAAGTAAACTGATTATTTGGATGCATGATTGTAATCTGCAAAGCGTAGTCAAGAAGGTAGGAGAGAATTTGTTGAGCCTGAGAGTGGTTTGCAGCTGTCTTTCCATCATTTGGTCCAATTTTTTAGTCATCTCATGTTGTGTCATGTCTTTAGAAGACACTTGGTATGTTATATGCTTGACAACAAATCCAAAGGTGTTGAAACAAAATATTATTTAATATCTCACTTTAGTTTGGTTTTGTTTGTCCCACTAAGTTAGACTTAATAAGCCTCATGTTGAAGTTCATACCTTTGTATT

Coding sequence (CDS)

ATGCCTTCCCTTCTCCTTCTCTTCTTCCTCCCTCTACATTTCTCCTCCGCCGCCCACCAAACCCTTCCCCCTGAGATCGCCGCCGCCTGCAACTCCACCCGCTTCCCGGACCAATGCGCATCCTCCATCTCCCAATCCAACCGTCTCCTCCAGAATCCCACTCCGCTGCAGATCATTCATGCCGCCCTCTACCTCTCCTCAGAGAATCTCAAAACTGCTAAATCCATGGTGAAGGCCATTCTCGATTCTGCCGAGAAGATTCCCAACCGCAACCGCACCGCGGCGGCGCTAAGCTGTATGGACGCGCTGGGTTATTCGGAGTACAGAATTTCTCTTTCCAACGACGCAATTCCGCTCGGTCGAATTAAAGACGCGCGCGCGTGGTTAAGCGCGTCGCTCCTCTACCAGACCGGTTGCCGGTCGGGGTTGAAGTACGTGAACGACACGCACGAAGTGGATCAAACGATGTCGTTTCTTGACAACTTAACCGTGCTGTCGAGCAACGCGCTGAGCATGGTTGTGGCGTACGATAATTTCGGGAATGAGACGGCGGCCTGGTTACCTCCGAGGACGGAGCGAGACGGGTTCTGGGAACACAGCAGTGGAACGGGGGAGGTGGTGAGAGGGGTGAGGATTCCGGCGAAGACGGAGGTGAATGTGACGGTGTGCAAGGATGGGAGTGAGGGGTGTTATCGAACGGTACAAGCAGCGGTGGAGGCGGCGCCGGGAGAGTCGGCGGTGAGGTTTGTGATACACATAAAGGAAGGGGTTTACGAAGAGAGTGTGAGAGTGGGGATAGAGAAGAAGAATTTGGTATTTTTGGGGGATGGGATGGGCAAAACGGTCATTACTGGATCTTTGAGTGTGGGTCAGCCTGGGATCTCGACTTACAATTCGGCCACCGTCGGAGTTCTTGGGGATGGATTCATTGCAAGTGAACTCACCATTCAAAACACAGCAGGTCCTGATGTCCACCAAGCTGTTGCCTTTAGATCAGACAGTGACCTCTCGATCATACATAACTGTGAGTTTATAAGCAATCAAGACACTCTCTACGCCCATTCCCTCCGTCAATTCTACAACTCCTGTCGAATTTTAGGCAATGTGGACTTCATTTTTGGCAACTCAGCCTCCTTCTTTCTAAACTGTCATATCTTAGTTCGTCCTCACCCACTCAATCCTCAACTGGGAGATGAGAATCCAATCACTGCACATGGGAGGACAGACCCAGCGCAGTCGACTGGGTTTGTGTTCCAAAACTGCTTGTTAAATGGCACTGAGGAGTTCATGGCATTGTACTATGCCAATCCTCAGAGGCATAGGACCTTCTTGGGGCGGCCTTGGAAAGAGTATTCGAGAACGGTTTTTATACATTGTGAACTTGAAGCTCTTGTTTCGTCATCGGGATGGATGCCATGGAGTGGGGATATTGGATTGAATACATTATATTATGGGGAGTTTGAGAATTTTGGAACTGGTTCAAATTCGTCAAAACGAGTTGCATGGAGTAATCAAATCCCTGCAGAGCATGCTTTTAGTTACTCTATACAAAATTTCATCCAAGGAAACGAGTGGATTCCTTCCTCTTAA

Protein sequence

MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIHAALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLGRIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFGNETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEAAPGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS
Homology
BLAST of MELO3C023253 vs. NCBI nr
Match: XP_008460901.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis melo] >KAA0040769.1 putative pectinesterase/pectinesterase inhibitor 51 [Cucumis melo var. makuwa] >TYK02085.1 putative pectinesterase/pectinesterase inhibitor 51 [Cucumis melo var. makuwa])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.2e-309
Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 0

Query: 1   MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIH 60
           MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIH
Sbjct: 1   MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIH 60

Query: 61  AALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLG 120
           AALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLG
Sbjct: 61  AALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLG 120

Query: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFG 180
           RIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFG
Sbjct: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFG 180

Query: 181 NETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEA 240
           NETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEA
Sbjct: 181 NETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEA 240

Query: 241 APGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSA 300
           APGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSA
Sbjct: 241 APGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSA 300

Query: 301 TVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFY 360
           TVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFY
Sbjct: 301 TVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFY 360

Query: 361 NSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQN 420
           NSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQN
Sbjct: 361 NSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQN 420

Query: 421 CLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLN 480
           CLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLN
Sbjct: 421 CLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLN 480

Query: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 530
           TLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS
Sbjct: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 529

BLAST of MELO3C023253 vs. NCBI nr
Match: XP_004147374.1 (probable pectinesterase/pectinesterase inhibitor 51 [Cucumis sativus] >KGN62147.1 hypothetical protein Csa_006578 [Cucumis sativus])

HSP 1 Score: 1009.6 bits (2609), Expect = 9.9e-291
Identity = 498/529 (94.14%), Postives = 509/529 (96.22%), Query Frame = 0

Query: 1   MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIH 60
           MPSLL LFFLPLHFSSAAHQTL P+IAAACN+TRFPDQCASSISQS+RL QNPT LQII 
Sbjct: 1   MPSLLFLFFLPLHFSSAAHQTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQ 60

Query: 61  AALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLG 120
           AALYLSS+NLKTAKSMVKAILDSAEKIPNRNRT  ALSCMDALGYSEYRISLSNDAIPLG
Sbjct: 61  AALYLSSDNLKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLG 120

Query: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFG 180
           RIKDARAWLSASLLYQTGCRSGLKYVNDT EVDQTM FLDNLTVLSSNALSMVVAYDNFG
Sbjct: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFG 180

Query: 181 NETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEA 240
           NETAAW PPRTERDGFWE SSGTGEVVRGVRIP KTE NVTVCKDGSEGCYRTVQAAV+A
Sbjct: 181 NETAAWRPPRTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGSEGCYRTVQAAVDA 240

Query: 241 APGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSA 300
           APGESAVRFVIHIKEGVYEESVRVG+EKKNLVFLGDGMGKTVITGSL+VGQPGISTYNSA
Sbjct: 241 APGESAVRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSA 300

Query: 301 TVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFY 360
           TVGVLGDGFIASELTIQNTAGPD HQAVAFRSDSDLSII NCEFISNQDTLYAHSLRQFY
Sbjct: 301 TVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFY 360

Query: 361 NSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQN 420
           NSCRILGNVDFIFGNSAS FLNCHIL+RPH LNPQLGDEN ITAHGRTDPAQSTGFVFQN
Sbjct: 361 NSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQN 420

Query: 421 CLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLN 480
           CL+NGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWM WSGDIGLN
Sbjct: 421 CLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLN 480

Query: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 530
           TLYYGEFENFGTGSNSSKRVAWSNQIPAE+ FSYS+QNFIQGNEWIPSS
Sbjct: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS 529

BLAST of MELO3C023253 vs. NCBI nr
Match: XP_038900555.1 (probable pectinesterase/pectinesterase inhibitor 51 [Benincasa hispida])

HSP 1 Score: 939.9 bits (2428), Expect = 9.7e-270
Identity = 461/529 (87.15%), Postives = 485/529 (91.68%), Query Frame = 0

Query: 1   MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIH 60
           MPSLL LFFLPLHFSSAAHQT+ P IAAAC +TRFPDQCASSISQS+RL QNPTPLQII 
Sbjct: 78  MPSLLFLFFLPLHFSSAAHQTILPGIAAACGATRFPDQCASSISQSSRLPQNPTPLQIIQ 137

Query: 61  AALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLG 120
           AA++LSSENLK AKSMVKAILDS+EK PNRNRTAAALSC++ LGYSEYRISLSNDAI +G
Sbjct: 138 AAVWLSSENLKIAKSMVKAILDSSEKSPNRNRTAAALSCLEGLGYSEYRISLSNDAIAIG 197

Query: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFG 180
           R KDARAWLSASLLYQTGCRSGLKYVNDT EVD+TMSFLDNL VLSSNALSM VAYD FG
Sbjct: 198 RNKDARAWLSASLLYQTGCRSGLKYVNDTREVDETMSFLDNLIVLSSNALSMAVAYDIFG 257

Query: 181 NETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEA 240
           NET AW PPRTERDGFWE S GT  V  G+ IPAKTE N TVCK+GSEGCY TVQAAVEA
Sbjct: 258 NETEAWGPPRTERDGFWERSGGTAVVGTGLGIPAKTEANATVCKEGSEGCYTTVQAAVEA 317

Query: 241 APGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSA 300
           AP  SAVRFVIHIKEGVYEESVRVG+EKKNLVFLGDGMGKTVITGSL+VGQPGISTY SA
Sbjct: 318 APENSAVRFVIHIKEGVYEESVRVGLEKKNLVFLGDGMGKTVITGSLNVGQPGISTYKSA 377

Query: 301 TVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFY 360
           TVGVLGDGF+AS LTIQNTAGPD HQAVAFRSDSDLSIIHNCEFI NQDTLYAHSLRQFY
Sbjct: 378 TVGVLGDGFMASRLTIQNTAGPDAHQAVAFRSDSDLSIIHNCEFIGNQDTLYAHSLRQFY 437

Query: 361 NSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQN 420
           NSCRI+GNVDFIFGNSAS F NCHILVRP  LNPQLGD+NPITAHGRTDPAQSTGFVFQN
Sbjct: 438 NSCRIVGNVDFIFGNSASIFQNCHILVRPRQLNPQLGDDNPITAHGRTDPAQSTGFVFQN 497

Query: 421 CLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLN 480
           CL+NGTEEFM+LYYANPQ H+TFLGRPWKEYSRT+FIHC+LEALVSSSGWMPWSGD GL 
Sbjct: 498 CLVNGTEEFMSLYYANPQAHKTFLGRPWKEYSRTIFIHCDLEALVSSSGWMPWSGDFGLK 557

Query: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 530
           TLYYGEFENF  GSNSSKRV WSNQIPAE+ FSYS+QNFIQGNEWIPSS
Sbjct: 558 TLYYGEFENFEIGSNSSKRVGWSNQIPAEYVFSYSVQNFIQGNEWIPSS 606

BLAST of MELO3C023253 vs. NCBI nr
Match: XP_008460902.1 (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis melo])

HSP 1 Score: 734.2 bits (1894), Expect = 8.1e-208
Identity = 362/521 (69.48%), Postives = 420/521 (80.61%), Query Frame = 0

Query: 10  LPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIHAALYLSSEN 69
           LP HF++    T   +I  AC STRFPDQC SS+S S+    NP+PLQI+ +A+ LSS+ 
Sbjct: 57  LPSHFTN----TPSDQILHACKSTRFPDQCGSSLSHSSXSPPNPSPLQILQSAIALSSDG 116

Query: 70  LKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLGRIKDARAWL 129
           L TA+S+VK ILDS+    N NRT AA +C++ L  S+YRISL+NDA+  GR KDARAWL
Sbjct: 117 LTTAQSLVKQILDSS--ATNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWL 176

Query: 130 SASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFGNETAAWLPP 189
           SASLLY   C S LKY NDT  VD+TM+FLD+L  LSSNALSM+VAYD +GN+TAAW PP
Sbjct: 177 SASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPP 236

Query: 190 RTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEAAPGESAV-R 249
           RTERDGFWE   G   +      PAK   + TVCKDGS GCY ++Q AV+AAP  +A  R
Sbjct: 237 RTERDGFWEGGGGGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARR 296

Query: 250 FVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDG 309
           FVIHIKEGVYEE VRV +EKKN+VFLGDGMGKTVITGSL+ GQPGISTYN+AT+GVLGDG
Sbjct: 297 FVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDG 356

Query: 310 FIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGN 369
           F+AS LTIQNTAGPD HQAVAFRSDSDLS+I +CEFI NQDTLYAHSLRQFY SC+I GN
Sbjct: 357 FMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGN 416

Query: 370 VDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEE 429
           VDFIFGNSAS F +C IL+RP  L P+ G+ N ITAHGRTDPAQSTGFVFQNCL+NGT+E
Sbjct: 417 VDFIFGNSASIFQDCEILIRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQE 476

Query: 430 FMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFE 489
           +M+LYY+ P+ H+ FLGRPWKEYSRTVFIHC LEALVS SGWMPWSGD  L TL+YGEFE
Sbjct: 477 YMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFE 536

Query: 490 NFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 530
           N G GS +S+RV WS+QIPA+H FSYS+QNFIQG+EWIPSS
Sbjct: 537 NSGVGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSS 571

BLAST of MELO3C023253 vs. NCBI nr
Match: KAG6571352.1 (putative pectinesterase/pectinesterase inhibitor 51, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 731.5 bits (1887), Expect = 5.2e-207
Identity = 358/521 (68.71%), Postives = 421/521 (80.81%), Query Frame = 0

Query: 10  LPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIHAALYLSSEN 69
           LP HF+++       E   AC +TRFPDQC SS+S S  L  NPT LQII +A+ +SS+ 
Sbjct: 59  LPSHFTNSPSS---EETFRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAITVSSDG 118

Query: 70  LKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLGRIKDARAWL 129
           L +AKSMVK ILDS+   P  NRT AA +C++ L +SEYRISL+NDA+  GR KDARAW+
Sbjct: 119 LASAKSMVKQILDSSTDSP--NRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWM 178

Query: 130 SASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFGNETAAWLPP 189
           SASLLY   C S LKY NDT  V++TM+FLD+L  L+SNALSM+ AYD  GN+TAAW PP
Sbjct: 179 SASLLYHYDCWSALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTPP 238

Query: 190 RTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEAAPGES-AVR 249
           RTERDGFWE SSG   +      P+K + + TVCKDG+ GCY+TVQ AV AAP  +   R
Sbjct: 239 RTERDGFWEGSSGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKR 298

Query: 250 FVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDG 309
           FVIHIKEGVY+E VRV +EKKN+VFLGDGMGKTVITGSL+VGQPGISTYN+ATVGV+GDG
Sbjct: 299 FVIHIKEGVYDEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDG 358

Query: 310 FIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGN 369
           F+AS LTIQNTAGPD HQAVAFRSDSDLS+I +CEF+ NQDTLYAHSLRQFY SC+I GN
Sbjct: 359 FMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGN 418

Query: 370 VDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEE 429
           VDFIFGNSAS F +C ILVRP  L P+ G+ N +TAHGRTDPAQ+TGFVFQNCL+NGT+E
Sbjct: 419 VDFIFGNSASVFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQE 478

Query: 430 FMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFE 489
           +MALYY+ P+ H+ FLGRPWKEYSRTVFIHC LEALVS +GWMPW+GD  L TLYYGEFE
Sbjct: 479 YMALYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFE 538

Query: 490 NFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 530
           N GTGS +S+RV WS+QIPA+H +SYS+QNFIQG+EWIPSS
Sbjct: 539 NSGTGSKTSERVEWSSQIPAQHVYSYSVQNFIQGDEWIPSS 574

BLAST of MELO3C023253 vs. ExPASy Swiss-Prot
Match: Q9LXD9 (Probable pectinesterase/pectinesterase inhibitor 51 OS=Arabidopsis thaliana OX=3702 GN=PME51 PE=2 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 1.0e-181
Identity = 313/514 (60.89%), Postives = 397/514 (77.24%), Query Frame = 0

Query: 21  TLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIHAALYLSSENLKTAKSMVKAI 80
           +L  +I  ACN+TR+PDQC SS+S+  R+  +P P+QIIH+A+  S +NLKTA+S +K+I
Sbjct: 40  SLAAQIRLACNATRYPDQCVSSLSEQGRVPPDPKPIQIIHSAISFSFQNLKTAQSKIKSI 99

Query: 81  LDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLGRIKDARAWLSASLLYQTGCR 140
           +DS+  + N NRT AA +C+  L YSE+R   ++ A+  G+IKDARAW+SA+L+YQ    
Sbjct: 100 VDSS--VGNLNRTNAANTCLQLLTYSEHRTQSTDQALTRGKIKDARAWMSAALVYQYDSW 159

Query: 141 SGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFGNETAAWLPPRTERDGFWEHS 200
           S LKYVNDT +V +TMSFLD L  ++SNALSM+V+YDNFG+  A+W  P TERDGFWE +
Sbjct: 160 SALKYVNDTSQVGETMSFLDGLIHVTSNALSMMVSYDNFGDNVASWTYPATERDGFWEKT 219

Query: 201 S-GTG-EVVRGVRI--PAKTEVNVTVCKDGSEGCYRTVQAAVEAAPGESAVR-FVIHIKE 260
             G G +   G+ +  P+  + +VTVCKDG  G Y+TVQ AV AAP ++ +R FVI I E
Sbjct: 220 GPGLGLDPSTGLNLGFPSGLKEDVTVCKDGKCG-YKTVQDAVNAAPEDNGMRKFVIKISE 279

Query: 261 GVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDGFIASELT 320
           GVYEE+V V  EKKN+VF+GDGMGKTVITGSL+ G PGI+TYN+ATVGV+GDGF+A +LT
Sbjct: 280 GVYEENVIVPFEKKNVVFIGDGMGKTVITGSLNAGMPGITTYNTATVGVVGDGFMARDLT 339

Query: 321 IQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGNVDFIFGN 380
            QNTAGPD HQAVAFRSDSD S+I NCEF+ NQDTLYAH LRQFY +CRI GNVDFIFGN
Sbjct: 340 FQNTAGPDAHQAVAFRSDSDFSLIENCEFLGNQDTLYAHGLRQFYKNCRIQGNVDFIFGN 399

Query: 381 SASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEEFMALYYA 440
           SA+ F +C IL+ P  +NP+ G++N +TA GR DP+QSTGFVF NCL+NGTEE+M L+ A
Sbjct: 400 SAAVFQDCEILIAPRQINPEKGEKNAVTAQGRIDPSQSTGFVFLNCLINGTEEYMKLFKA 459

Query: 441 NPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFENFGTGSN 500
           NP+ H+ FLGRPWK+YSRTVFI C LEAL++  GW+PWSGD  L TLYYGE +N G GS+
Sbjct: 460 NPKVHKNFLGRPWKDYSRTVFIGCNLEALITPDGWLPWSGDFALKTLYYGESKNTGPGSD 519

Query: 501 SSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 530
            S+RV+WS+QIP EH   YS+ NFIQ +EW   S
Sbjct: 520 RSQRVSWSSQIPDEHVHVYSVANFIQADEWASMS 550

BLAST of MELO3C023253 vs. ExPASy Swiss-Prot
Match: Q8L7Q7 (Probable pectinesterase/pectinesterase inhibitor 64 OS=Arabidopsis thaliana OX=3702 GN=PME64 PE=2 SV=2)

HSP 1 Score: 602.4 bits (1552), Expect = 4.8e-171
Identity = 298/514 (57.98%), Postives = 385/514 (74.90%), Query Frame = 0

Query: 23  PPEIA---AACNSTRFPDQCASSISQSNRLLQNPTPLQIIHAALYLSSENLKTAKSMVKA 82
           PP IA    ACN+TRFPD C +S+S+  ++  +P P+QIIH+A+ +S ENLK+ +S +++
Sbjct: 87  PPPIAQIRLACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKIQS 146

Query: 83  ILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLGRIKDARAWLSASLLYQTGC 142
           ILDS+    NRNRT  A  C++ L YS++R   ++ A+  G IKDARAW+SA+L YQ  C
Sbjct: 147 ILDSS--AGNRNRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDC 206

Query: 143 RSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFGNETAAWLPPRTERDGFWEH 202
            SGLK VNDT +V  T++F + L  L+ NALSM++++D+FG++  +W+ P TERDGFWE 
Sbjct: 207 WSGLKTVNDTKQVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEK 266

Query: 203 S-----SGTGEVVRGVRIPAKTEVNVTVCKDGSEGC-YRTVQAAVEAAPGES-AVRFVIH 262
           +     SGTG     +  P+    +VTVCK+G + C Y+TVQ AV++AP  +  V+FVI 
Sbjct: 267 AGPSLGSGTG-TEASLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIR 326

Query: 263 IKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDGFIAS 322
           I+EGVYEE+VRV  EKKN+VF+GDGMGKTVITGSL+VGQPG++T+ SATVGVLGDGF+A 
Sbjct: 327 IREGVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMAR 386

Query: 323 ELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGNVDFI 382
           +LTI+NTAG D HQAVAFRSDSD S++ NCEF+ NQDTLYAHSLRQFY  CRI GNVDFI
Sbjct: 387 DLTIENTAGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFI 446

Query: 383 FGNSASFFLNCHILV-RPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEEFMA 442
           FGNSA+ F +C IL+   H    Q G  N ITAHGR D +QSTGFVF NC +NGTEE+M 
Sbjct: 447 FGNSAAVFQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMK 506

Query: 443 LYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFENFG 502
            + ANP+ H+ FLGRPWKE+SRTVF++C LE+L+S  GWMPW+GD  L TLYYGE++N G
Sbjct: 507 EFQANPEGHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTG 566

Query: 503 TGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEW 526
            GS  S RV WS++IP +H   YS+ NFIQ +EW
Sbjct: 567 PGSVRSSRVPWSSEIPEKHVDVYSVANFIQADEW 597

BLAST of MELO3C023253 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.0e-88
Identity = 199/536 (37.13%), Postives = 286/536 (53.36%), Query Frame = 0

Query: 3   SLLLLFFLPLHFSSAAHQTL---PPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQII 62
           +L +L  + L   S +H +    P E+ + C  T  P  C   ++Q   +          
Sbjct: 2   ALRILITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFY 61

Query: 63  HAALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPL 122
             +L L+ E   TA+S    +     K  N    AA   C +    +  +++ ++++ P 
Sbjct: 62  KISLQLALERATTAQSRTYTL---GSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPG 121

Query: 123 GRIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSM-VVAYD- 182
               D + WLS +L     CR+ L+ +     V   +S  +N+T L SN LS+  V Y+ 
Sbjct: 122 CTKVDKQTWLSTALTNLETCRASLEDLGVPEYVLPLLS--NNVTKLISNTLSLNKVPYNE 181

Query: 183 -NFGNETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQA 242
            ++ +    W+ P   +                  +      N+ V +DGS G  +T+Q 
Sbjct: 182 PSYKDGFPTWVKPGDRK-----------------LLQTTPRANIVVAQDGS-GNVKTIQE 241

Query: 243 AVEAAPGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGIST 302
           AV AA      R+VI+IK G Y E++ V +  KN++F+GDG+GKT+ITGS SVG  G +T
Sbjct: 242 AVAAASRAGGSRYVIYIKAGTYNENIEVKL--KNIMFVGDGIGKTIITGSKSVG-GGATT 301

Query: 303 YNSATVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSL 362
           + SATV V+GD FIA ++TI+NTAGP+ HQAVA RS SDLS+ + C F   QDTLY HS 
Sbjct: 302 FKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQ 361

Query: 363 RQFYNSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGF 422
           RQFY  C I G VDFIFGN+A    NC+I  R  P        N +TA GRTDP QSTG 
Sbjct: 362 RQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPP-----NRTNTLTAQGRTDPNQSTGI 421

Query: 423 VFQNCLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGD 482
           +  NC +    +   +        +TFLGRPWK+YSRTV+I   L++L++ +GWM WSGD
Sbjct: 422 IIHNCRVTAASDLKPV----QSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGD 481

Query: 483 IGLNTLYYGEFENFGTGSNSSKRVAWSNQ---IPAEHAFSYSIQNFIQGNEWIPSS 530
             LNTLYY E+ N G GS+++ RV W              +++ NFI GN W+P++
Sbjct: 482 FALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPAT 502

BLAST of MELO3C023253 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 4.6e-81
Identity = 185/516 (35.85%), Postives = 279/516 (54.07%), Query Frame = 0

Query: 26  IAAACNSTRFPDQCASSISQSNRLLQNPTPL--QIIHAALYLSSENLKTAKSMVKAILDS 85
           I   C  T + + C  ++ +  +   +P  L     +A +   S+  K +++M++   D 
Sbjct: 57  IKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDP 116

Query: 86  AEKIPNRNRTAAALSCMDALGYSEYRISLSNDAI---PLGRIKDA----RAWLSASLLYQ 145
             K+       A   C + + Y+   +S S + +      ++ +A    R WLSA++ ++
Sbjct: 117 RAKM-------ALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHE 176

Query: 146 TGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFGNETAAWLPPRTERDGF 205
             C  G +     +  +     L     L+ N L+MV    N+  +    +P    R   
Sbjct: 177 QTCLDGFQGTQG-NAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQ--IPEMNSRRLL 236

Query: 206 -WEHSSGTGEVVRGVRIPAKTEV--NVTVCKDGSEGCYRTVQAAVEAAPGESAVRFVIHI 265
             E  S      R +     +EV  ++ V +DGS G Y+T+  A+   P +    FV+HI
Sbjct: 237 SQEFPSWMDARARRLLNAPMSEVKPDIVVAQDGS-GQYKTINEALNFVPKKKNTTFVVHI 296

Query: 266 KEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDGFIASE 325
           KEG+Y+E V+V     +LVF+GDG  KTVI+GS S  + GI+TY +ATV ++GD FIA  
Sbjct: 297 KEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSY-KDGITTYKTATVAIVGDHFIAKN 356

Query: 326 LTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGNVDFIF 385
           +  +NTAG   HQAVA R  +D SI +NC+F   QDTLYAHS RQFY  C I G +DF+F
Sbjct: 357 IAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLF 416

Query: 386 GNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEEFMALY 445
           G++A+ F NC +LVR     P L    PITAHGR DP +STGFV Q C + G  +++A+ 
Sbjct: 417 GDAAAVFQNCTLLVR----KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAV- 476

Query: 446 YANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFENFGTG 505
               ++ +T+LGRPWKEYSRT+ ++  +   V   GW PW G+ GLNTL+Y E +N G G
Sbjct: 477 ---KEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPG 536

Query: 506 SNSSKRVAWS--NQIPAEHAFSYSIQNFIQGNEWIP 528
           +  +KRV W    ++  E    ++   +IQG+ WIP
Sbjct: 537 AAITKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIP 552

BLAST of MELO3C023253 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 296.6 bits (758), Expect = 5.6e-79
Identity = 178/517 (34.43%), Postives = 278/517 (53.77%), Query Frame = 0

Query: 30  CNSTRFPDQCASSISQSNRLLQNPTPLQIIHAALYLSSENLKTAKSMVKAILDSAEKIPN 89
           C  T +   C  ++ ++ +      P++++  A  ++ + +  A    + I++    +  
Sbjct: 62  CAPTDYRKTCEDTLIKNGK--NTTDPMELVKTAFNVTMKQITDAAKKSQTIME----LQK 121

Query: 90  RNRTAAAL-SCMDALGYSEYRISLSNDAIPLGRIK---------DARAWLSASLLYQTGC 149
            +RT  AL  C + + Y+     LSN    LG+ +         + R WLSA++ ++  C
Sbjct: 122 DSRTRMALDQCKELMDYA--LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETC 181

Query: 150 RSGLKYVNDTHEVDQTM-SFLDNLTVLSSNALSMVVAYDNFGNETAAWLPPRTER----D 209
             G  +        +TM   L     L+ N L+++    NF  +    +P    R    +
Sbjct: 182 LEG--FQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQ--IPGLNSRRLLAE 241

Query: 210 GF--WEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEAAPGESAVRFVIH 269
           GF  W    G  ++++     +  + ++ V +DGS G Y+T+  A++  P +    FV+H
Sbjct: 242 GFPSWVDQRGR-KLLQAAAAYSDVKPDIVVAQDGS-GQYKTINEALQFVPKKRNTTFVVH 301

Query: 270 IKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDGFIAS 329
           IK G+Y+E V+V     +LVF+GDG  KT+I+G+ +  + GI+TY +ATV ++G+ FIA 
Sbjct: 302 IKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNY-KDGITTYRTATVAIVGNYFIAK 361

Query: 330 ELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGNVDFI 389
            +  +NTAG   HQAVA R  SD SI  NC F   QDTLY HS RQF+  C I G +DF+
Sbjct: 362 NIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFL 421

Query: 390 FGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEEFMAL 449
           FG++A+ F NC +LVR     P      PITAHGR DP +STGFVFQ C + G  +++A+
Sbjct: 422 FGDAAAVFQNCTLLVR----KPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAV 481

Query: 450 YYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFENFGT 509
                +  + +LGRPWKEYSRT+ ++  +   V   GW PW GD GL TL+Y E +N G 
Sbjct: 482 ----KETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGP 541

Query: 510 GSNSSKRVAWS--NQIPAEHAFSYSIQNFIQGNEWIP 528
           GS  + RV W+    +  E    ++   +IQG++WIP
Sbjct: 542 GSALANRVTWAGIKTLSEEDILKFTPAQYIQGDDWIP 555

BLAST of MELO3C023253 vs. ExPASy TrEMBL
Match: A0A5A7THL0 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00720 PE=3 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 5.9e-310
Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 0

Query: 1   MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIH 60
           MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIH
Sbjct: 1   MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIH 60

Query: 61  AALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLG 120
           AALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLG
Sbjct: 61  AALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLG 120

Query: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFG 180
           RIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFG
Sbjct: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFG 180

Query: 181 NETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEA 240
           NETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEA
Sbjct: 181 NETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEA 240

Query: 241 APGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSA 300
           APGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSA
Sbjct: 241 APGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSA 300

Query: 301 TVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFY 360
           TVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFY
Sbjct: 301 TVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFY 360

Query: 361 NSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQN 420
           NSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQN
Sbjct: 361 NSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQN 420

Query: 421 CLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLN 480
           CLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLN
Sbjct: 421 CLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLN 480

Query: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 530
           TLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS
Sbjct: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 529

BLAST of MELO3C023253 vs. ExPASy TrEMBL
Match: A0A1S3CEP7 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499642 PE=3 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 5.9e-310
Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 0

Query: 1   MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIH 60
           MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIH
Sbjct: 1   MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIH 60

Query: 61  AALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLG 120
           AALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLG
Sbjct: 61  AALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLG 120

Query: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFG 180
           RIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFG
Sbjct: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFG 180

Query: 181 NETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEA 240
           NETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEA
Sbjct: 181 NETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEA 240

Query: 241 APGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSA 300
           APGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSA
Sbjct: 241 APGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSA 300

Query: 301 TVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFY 360
           TVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFY
Sbjct: 301 TVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFY 360

Query: 361 NSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQN 420
           NSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQN
Sbjct: 361 NSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQN 420

Query: 421 CLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLN 480
           CLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLN
Sbjct: 421 CLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLN 480

Query: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 530
           TLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS
Sbjct: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 529

BLAST of MELO3C023253 vs. ExPASy TrEMBL
Match: A0A0A0LMY9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G302140 PE=3 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 4.8e-291
Identity = 498/529 (94.14%), Postives = 509/529 (96.22%), Query Frame = 0

Query: 1   MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIH 60
           MPSLL LFFLPLHFSSAAHQTL P+IAAACN+TRFPDQCASSISQS+RL QNPT LQII 
Sbjct: 1   MPSLLFLFFLPLHFSSAAHQTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQ 60

Query: 61  AALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLG 120
           AALYLSS+NLKTAKSMVKAILDSAEKIPNRNRT  ALSCMDALGYSEYRISLSNDAIPLG
Sbjct: 61  AALYLSSDNLKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLG 120

Query: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFG 180
           RIKDARAWLSASLLYQTGCRSGLKYVNDT EVDQTM FLDNLTVLSSNALSMVVAYDNFG
Sbjct: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFG 180

Query: 181 NETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEA 240
           NETAAW PPRTERDGFWE SSGTGEVVRGVRIP KTE NVTVCKDGSEGCYRTVQAAV+A
Sbjct: 181 NETAAWRPPRTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGSEGCYRTVQAAVDA 240

Query: 241 APGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSA 300
           APGESAVRFVIHIKEGVYEESVRVG+EKKNLVFLGDGMGKTVITGSL+VGQPGISTYNSA
Sbjct: 241 APGESAVRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSA 300

Query: 301 TVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFY 360
           TVGVLGDGFIASELTIQNTAGPD HQAVAFRSDSDLSII NCEFISNQDTLYAHSLRQFY
Sbjct: 301 TVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFY 360

Query: 361 NSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQN 420
           NSCRILGNVDFIFGNSAS FLNCHIL+RPH LNPQLGDEN ITAHGRTDPAQSTGFVFQN
Sbjct: 361 NSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQN 420

Query: 421 CLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLN 480
           CL+NGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWM WSGDIGLN
Sbjct: 421 CLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLN 480

Query: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 530
           TLYYGEFENFGTGSNSSKRVAWSNQIPAE+ FSYS+QNFIQGNEWIPSS
Sbjct: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS 529

BLAST of MELO3C023253 vs. ExPASy TrEMBL
Match: A0A1S3CD05 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499643 PE=3 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.9e-208
Identity = 362/521 (69.48%), Postives = 420/521 (80.61%), Query Frame = 0

Query: 10  LPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIHAALYLSSEN 69
           LP HF++    T   +I  AC STRFPDQC SS+S S+    NP+PLQI+ +A+ LSS+ 
Sbjct: 57  LPSHFTN----TPSDQILHACKSTRFPDQCGSSLSHSSXSPPNPSPLQILQSAIALSSDG 116

Query: 70  LKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLGRIKDARAWL 129
           L TA+S+VK ILDS+    N NRT AA +C++ L  S+YRISL+NDA+  GR KDARAWL
Sbjct: 117 LTTAQSLVKQILDSS--ATNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWL 176

Query: 130 SASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFGNETAAWLPP 189
           SASLLY   C S LKY NDT  VD+TM+FLD+L  LSSNALSM+VAYD +GN+TAAW PP
Sbjct: 177 SASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPP 236

Query: 190 RTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEAAPGESAV-R 249
           RTERDGFWE   G   +      PAK   + TVCKDGS GCY ++Q AV+AAP  +A  R
Sbjct: 237 RTERDGFWEGGGGGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARR 296

Query: 250 FVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDG 309
           FVIHIKEGVYEE VRV +EKKN+VFLGDGMGKTVITGSL+ GQPGISTYN+AT+GVLGDG
Sbjct: 297 FVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDG 356

Query: 310 FIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGN 369
           F+AS LTIQNTAGPD HQAVAFRSDSDLS+I +CEFI NQDTLYAHSLRQFY SC+I GN
Sbjct: 357 FMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGN 416

Query: 370 VDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEE 429
           VDFIFGNSAS F +C IL+RP  L P+ G+ N ITAHGRTDPAQSTGFVFQNCL+NGT+E
Sbjct: 417 VDFIFGNSASIFQDCEILIRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQE 476

Query: 430 FMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFE 489
           +M+LYY+ P+ H+ FLGRPWKEYSRTVFIHC LEALVS SGWMPWSGD  L TL+YGEFE
Sbjct: 477 YMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFE 536

Query: 490 NFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 530
           N G GS +S+RV WS+QIPA+H FSYS+QNFIQG+EWIPSS
Sbjct: 537 NSGVGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSS 571

BLAST of MELO3C023253 vs. ExPASy TrEMBL
Match: A0A6J1EV58 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438335 PE=3 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 3.3e-207
Identity = 358/521 (68.71%), Postives = 421/521 (80.81%), Query Frame = 0

Query: 10  LPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIHAALYLSSEN 69
           LP HF+++       E   AC +TRFPDQC SS+S S  L  NPT LQII +A+ +SS+ 
Sbjct: 59  LPSHFTNSPSS---EETFRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDG 118

Query: 70  LKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLGRIKDARAWL 129
           L +AKSMVK ILDS+   P  NRT AA +C++ L +SEYRISL+NDA+  GR KDARAW+
Sbjct: 119 LASAKSMVKQILDSSTDSP--NRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWM 178

Query: 130 SASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFGNETAAWLPP 189
           SASLLY   C S LKY NDT  V++TM+FLD+L  L+SNALSM+ AYD  GN+TAAW PP
Sbjct: 179 SASLLYHYDCWSALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTPP 238

Query: 190 RTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEAAPGES-AVR 249
           RTERDGFWE SSG   +      P+K + + TVCKDG+ GCY+TVQ AV AAP  +   R
Sbjct: 239 RTERDGFWEGSSGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKR 298

Query: 250 FVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDG 309
           FVIHIKEGVY+E VRV +EKKN+VFLGDGMGKTVITGSL+VGQPGISTYN+ATVGV+GDG
Sbjct: 299 FVIHIKEGVYDEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDG 358

Query: 310 FIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGN 369
           F+AS LTIQNTAGPD HQAVAFRSDSDLS+I +CEF+ NQDTLYAHSLRQFY SC+I GN
Sbjct: 359 FMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGN 418

Query: 370 VDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEE 429
           VDFIFGNSAS F +C ILVRP  L P+ G+ N +TAHGRTDPAQ+TGFVFQNCL+NGT+E
Sbjct: 419 VDFIFGNSASVFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLVNGTQE 478

Query: 430 FMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFE 489
           +MALYY+ P+ H+ FLGRPWKEYSRTVFIHC LEALVS +GWMPW+GD  L TLYYGEFE
Sbjct: 479 YMALYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFE 538

Query: 490 NFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 530
           N GTGS +S+RV WS+QIPA+H +SYS+QNFIQG+EWIPSS
Sbjct: 539 NSGTGSKTSERVEWSSQIPAQHVYSYSVQNFIQGDEWIPSS 574

BLAST of MELO3C023253 vs. TAIR 10
Match: AT5G09760.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 637.9 bits (1644), Expect = 7.3e-183
Identity = 313/514 (60.89%), Postives = 397/514 (77.24%), Query Frame = 0

Query: 21  TLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIHAALYLSSENLKTAKSMVKAI 80
           +L  +I  ACN+TR+PDQC SS+S+  R+  +P P+QIIH+A+  S +NLKTA+S +K+I
Sbjct: 40  SLAAQIRLACNATRYPDQCVSSLSEQGRVPPDPKPIQIIHSAISFSFQNLKTAQSKIKSI 99

Query: 81  LDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLGRIKDARAWLSASLLYQTGCR 140
           +DS+  + N NRT AA +C+  L YSE+R   ++ A+  G+IKDARAW+SA+L+YQ    
Sbjct: 100 VDSS--VGNLNRTNAANTCLQLLTYSEHRTQSTDQALTRGKIKDARAWMSAALVYQYDSW 159

Query: 141 SGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFGNETAAWLPPRTERDGFWEHS 200
           S LKYVNDT +V +TMSFLD L  ++SNALSM+V+YDNFG+  A+W  P TERDGFWE +
Sbjct: 160 SALKYVNDTSQVGETMSFLDGLIHVTSNALSMMVSYDNFGDNVASWTYPATERDGFWEKT 219

Query: 201 S-GTG-EVVRGVRI--PAKTEVNVTVCKDGSEGCYRTVQAAVEAAPGESAVR-FVIHIKE 260
             G G +   G+ +  P+  + +VTVCKDG  G Y+TVQ AV AAP ++ +R FVI I E
Sbjct: 220 GPGLGLDPSTGLNLGFPSGLKEDVTVCKDGKCG-YKTVQDAVNAAPEDNGMRKFVIKISE 279

Query: 261 GVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDGFIASELT 320
           GVYEE+V V  EKKN+VF+GDGMGKTVITGSL+ G PGI+TYN+ATVGV+GDGF+A +LT
Sbjct: 280 GVYEENVIVPFEKKNVVFIGDGMGKTVITGSLNAGMPGITTYNTATVGVVGDGFMARDLT 339

Query: 321 IQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGNVDFIFGN 380
            QNTAGPD HQAVAFRSDSD S+I NCEF+ NQDTLYAH LRQFY +CRI GNVDFIFGN
Sbjct: 340 FQNTAGPDAHQAVAFRSDSDFSLIENCEFLGNQDTLYAHGLRQFYKNCRIQGNVDFIFGN 399

Query: 381 SASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEEFMALYYA 440
           SA+ F +C IL+ P  +NP+ G++N +TA GR DP+QSTGFVF NCL+NGTEE+M L+ A
Sbjct: 400 SAAVFQDCEILIAPRQINPEKGEKNAVTAQGRIDPSQSTGFVFLNCLINGTEEYMKLFKA 459

Query: 441 NPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFENFGTGSN 500
           NP+ H+ FLGRPWK+YSRTVFI C LEAL++  GW+PWSGD  L TLYYGE +N G GS+
Sbjct: 460 NPKVHKNFLGRPWKDYSRTVFIGCNLEALITPDGWLPWSGDFALKTLYYGESKNTGPGSD 519

Query: 501 SSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 530
            S+RV+WS+QIP EH   YS+ NFIQ +EW   S
Sbjct: 520 RSQRVSWSSQIPDEHVHVYSVANFIQADEWASMS 550

BLAST of MELO3C023253 vs. TAIR 10
Match: AT5G64640.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 602.4 bits (1552), Expect = 3.4e-172
Identity = 298/514 (57.98%), Postives = 385/514 (74.90%), Query Frame = 0

Query: 23  PPEIA---AACNSTRFPDQCASSISQSNRLLQNPTPLQIIHAALYLSSENLKTAKSMVKA 82
           PP IA    ACN+TRFPD C +S+S+  ++  +P P+QIIH+A+ +S ENLK+ +S +++
Sbjct: 87  PPPIAQIRLACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKIQS 146

Query: 83  ILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLGRIKDARAWLSASLLYQTGC 142
           ILDS+    NRNRT  A  C++ L YS++R   ++ A+  G IKDARAW+SA+L YQ  C
Sbjct: 147 ILDSS--AGNRNRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDC 206

Query: 143 RSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFGNETAAWLPPRTERDGFWEH 202
            SGLK VNDT +V  T++F + L  L+ NALSM++++D+FG++  +W+ P TERDGFWE 
Sbjct: 207 WSGLKTVNDTKQVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEK 266

Query: 203 S-----SGTGEVVRGVRIPAKTEVNVTVCKDGSEGC-YRTVQAAVEAAPGES-AVRFVIH 262
           +     SGTG     +  P+    +VTVCK+G + C Y+TVQ AV++AP  +  V+FVI 
Sbjct: 267 AGPSLGSGTG-TEASLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIR 326

Query: 263 IKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDGFIAS 322
           I+EGVYEE+VRV  EKKN+VF+GDGMGKTVITGSL+VGQPG++T+ SATVGVLGDGF+A 
Sbjct: 327 IREGVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMAR 386

Query: 323 ELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGNVDFI 382
           +LTI+NTAG D HQAVAFRSDSD S++ NCEF+ NQDTLYAHSLRQFY  CRI GNVDFI
Sbjct: 387 DLTIENTAGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFI 446

Query: 383 FGNSASFFLNCHILV-RPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEEFMA 442
           FGNSA+ F +C IL+   H    Q G  N ITAHGR D +QSTGFVF NC +NGTEE+M 
Sbjct: 447 FGNSAAVFQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMK 506

Query: 443 LYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFENFG 502
            + ANP+ H+ FLGRPWKE+SRTVF++C LE+L+S  GWMPW+GD  L TLYYGE++N G
Sbjct: 507 EFQANPEGHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTG 566

Query: 503 TGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEW 526
            GS  S RV WS++IP +H   YS+ NFIQ +EW
Sbjct: 567 PGSVRSSRVPWSSEIPEKHVDVYSVANFIQADEW 597

BLAST of MELO3C023253 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 303.5 bits (776), Expect = 3.3e-82
Identity = 185/516 (35.85%), Postives = 279/516 (54.07%), Query Frame = 0

Query: 26  IAAACNSTRFPDQCASSISQSNRLLQNPTPL--QIIHAALYLSSENLKTAKSMVKAILDS 85
           I   C  T + + C  ++ +  +   +P  L     +A +   S+  K +++M++   D 
Sbjct: 57  IKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDP 116

Query: 86  AEKIPNRNRTAAALSCMDALGYSEYRISLSNDAI---PLGRIKDA----RAWLSASLLYQ 145
             K+       A   C + + Y+   +S S + +      ++ +A    R WLSA++ ++
Sbjct: 117 RAKM-------ALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHE 176

Query: 146 TGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFGNETAAWLPPRTERDGF 205
             C  G +     +  +     L     L+ N L+MV    N+  +    +P    R   
Sbjct: 177 QTCLDGFQGTQG-NAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQ--IPEMNSRRLL 236

Query: 206 -WEHSSGTGEVVRGVRIPAKTEV--NVTVCKDGSEGCYRTVQAAVEAAPGESAVRFVIHI 265
             E  S      R +     +EV  ++ V +DGS G Y+T+  A+   P +    FV+HI
Sbjct: 237 SQEFPSWMDARARRLLNAPMSEVKPDIVVAQDGS-GQYKTINEALNFVPKKKNTTFVVHI 296

Query: 266 KEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDGFIASE 325
           KEG+Y+E V+V     +LVF+GDG  KTVI+GS S  + GI+TY +ATV ++GD FIA  
Sbjct: 297 KEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSY-KDGITTYKTATVAIVGDHFIAKN 356

Query: 326 LTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGNVDFIF 385
           +  +NTAG   HQAVA R  +D SI +NC+F   QDTLYAHS RQFY  C I G +DF+F
Sbjct: 357 IAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLF 416

Query: 386 GNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEEFMALY 445
           G++A+ F NC +LVR     P L    PITAHGR DP +STGFV Q C + G  +++A+ 
Sbjct: 417 GDAAAVFQNCTLLVR----KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAV- 476

Query: 446 YANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFENFGTG 505
               ++ +T+LGRPWKEYSRT+ ++  +   V   GW PW G+ GLNTL+Y E +N G G
Sbjct: 477 ---KEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPG 536

Query: 506 SNSSKRVAWS--NQIPAEHAFSYSIQNFIQGNEWIP 528
           +  +KRV W    ++  E    ++   +IQG+ WIP
Sbjct: 537 AAITKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIP 552

BLAST of MELO3C023253 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 296.6 bits (758), Expect = 4.0e-80
Identity = 178/517 (34.43%), Postives = 278/517 (53.77%), Query Frame = 0

Query: 30  CNSTRFPDQCASSISQSNRLLQNPTPLQIIHAALYLSSENLKTAKSMVKAILDSAEKIPN 89
           C  T +   C  ++ ++ +      P++++  A  ++ + +  A    + I++    +  
Sbjct: 62  CAPTDYRKTCEDTLIKNGK--NTTDPMELVKTAFNVTMKQITDAAKKSQTIME----LQK 121

Query: 90  RNRTAAAL-SCMDALGYSEYRISLSNDAIPLGRIK---------DARAWLSASLLYQTGC 149
            +RT  AL  C + + Y+     LSN    LG+ +         + R WLSA++ ++  C
Sbjct: 122 DSRTRMALDQCKELMDYA--LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETC 181

Query: 150 RSGLKYVNDTHEVDQTM-SFLDNLTVLSSNALSMVVAYDNFGNETAAWLPPRTER----D 209
             G  +        +TM   L     L+ N L+++    NF  +    +P    R    +
Sbjct: 182 LEG--FQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQ--IPGLNSRRLLAE 241

Query: 210 GF--WEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEAAPGESAVRFVIH 269
           GF  W    G  ++++     +  + ++ V +DGS G Y+T+  A++  P +    FV+H
Sbjct: 242 GFPSWVDQRGR-KLLQAAAAYSDVKPDIVVAQDGS-GQYKTINEALQFVPKKRNTTFVVH 301

Query: 270 IKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDGFIAS 329
           IK G+Y+E V+V     +LVF+GDG  KT+I+G+ +  + GI+TY +ATV ++G+ FIA 
Sbjct: 302 IKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNY-KDGITTYRTATVAIVGNYFIAK 361

Query: 330 ELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGNVDFI 389
            +  +NTAG   HQAVA R  SD SI  NC F   QDTLY HS RQF+  C I G +DF+
Sbjct: 362 NIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFL 421

Query: 390 FGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEEFMAL 449
           FG++A+ F NC +LVR     P      PITAHGR DP +STGFVFQ C + G  +++A+
Sbjct: 422 FGDAAAVFQNCTLLVR----KPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAV 481

Query: 450 YYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFENFGT 509
                +  + +LGRPWKEYSRT+ ++  +   V   GW PW GD GL TL+Y E +N G 
Sbjct: 482 ----KETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGP 541

Query: 510 GSNSSKRVAWS--NQIPAEHAFSYSIQNFIQGNEWIP 528
           GS  + RV W+    +  E    ++   +IQG++WIP
Sbjct: 542 GSALANRVTWAGIKTLSEEDILKFTPAQYIQGDDWIP 555

BLAST of MELO3C023253 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 294.7 bits (753), Expect = 1.5e-79
Identity = 193/516 (37.40%), Postives = 279/516 (54.07%), Query Frame = 0

Query: 25  EIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIHAALYLSSENLKTAKSMV--KAILD 84
           ++ A C+ T  P  C   ++ ++    N  P++         SE LK +  +V  +AIL 
Sbjct: 29  DVKAWCSQTPNPKPCEYFLTHNS----NNEPIK-------SESEFLKISMKLVLDRAILA 88

Query: 85  SAE------KIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLGRIKDARAWLSASLLYQ 144
                    K  +    AA   C+     +  +I+ + D        DA+ WLS +L   
Sbjct: 89  KTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNL 148

Query: 145 TGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFGNETAAWLPPRTERDGF 204
             CR+G   +  T  V   MS  +N++ L  N L++     N+        PP  E+DGF
Sbjct: 149 DTCRAGFLELGVTDIVLPLMS--NNVSNLLCNTLAINKVPFNY-------TPP--EKDGF 208

Query: 205 WEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEAAPGESAVRFVIHIKEG 264
               S      R +   +  + N  V KDGS G ++T++ A++AA G    RFVI++K+G
Sbjct: 209 ---PSWVKPGDRKLLQSSTPKDNAVVAKDGS-GNFKTIKEAIDAASGSG--RFVIYVKQG 268

Query: 265 VYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSATVGVLGDGFIASELTI 324
           VY E++   I KKN++  GDG+GKT+ITGS SVG  G +T+NSATV  +GDGFIA  +T 
Sbjct: 269 VYSENLE--IRKKNVMLRGDGIGKTIITGSKSVG-GGTTTFNSATVAAVGDGFIARGITF 328

Query: 325 QNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFYNSCRILGNVDFIFGNS 384
           +NTAG    QAVA RS SDLS+ + C F + QDTLY HS RQFY  C + G VDFIFGN+
Sbjct: 329 RNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNA 388

Query: 385 ASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQNCLLNGTEEFMALYYAN 444
           A+   NC+I  R     P+    N ITA GR+DP Q+TG +  N  +    +   +  + 
Sbjct: 389 AAVLQNCNIFAR----RPR-SKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGST 448

Query: 445 PQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLNTLYYGEFENFGTGSNS 504
               +T+LGRPW++YSRTVF+   L++L+   GW+ W G+  L TL+Y EF+N G G+++
Sbjct: 449 ----KTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGAST 504

Query: 505 SKRVAWSN---QIPAEHAFSYSIQNFIQGNEWIPSS 530
           S RV W        A  A  +++  F+ G  WIPSS
Sbjct: 509 SGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS 504

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008460901.11.2e-309100.00PREDICTED: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis melo] >K... [more]
XP_004147374.19.9e-29194.14probable pectinesterase/pectinesterase inhibitor 51 [Cucumis sativus] >KGN62147.... [more]
XP_038900555.19.7e-27087.15probable pectinesterase/pectinesterase inhibitor 51 [Benincasa hispida][more]
XP_008460902.18.1e-20869.48PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor... [more]
KAG6571352.15.2e-20768.71putative pectinesterase/pectinesterase inhibitor 51, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
Q9LXD91.0e-18160.89Probable pectinesterase/pectinesterase inhibitor 51 OS=Arabidopsis thaliana OX=3... [more]
Q8L7Q74.8e-17157.98Probable pectinesterase/pectinesterase inhibitor 64 OS=Arabidopsis thaliana OX=3... [more]
O048873.0e-8837.13Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
Q3E8Z84.6e-8135.85Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q8GX865.6e-7934.43Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A5A7THL05.9e-310100.00Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00720... [more]
A0A1S3CEP75.9e-310100.00Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499642 PE=3 SV=1[more]
A0A0A0LMY94.8e-29194.14Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G302140 PE=3 SV=1[more]
A0A1S3CD053.9e-20869.48Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499643 PE=3 SV=1[more]
A0A6J1EV583.3e-20768.71Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438335 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G09760.17.3e-18360.89Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G64640.13.4e-17257.98Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.13.3e-8235.85Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.14.0e-8034.43Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G45220.11.5e-7937.40Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 21..172
e-value: 1.4E-17
score: 74.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 26..172
e-value: 8.5E-19
score: 68.4
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 4..172
e-value: 1.0E-14
score: 52.2
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 219..523
e-value: 9.8E-116
score: 385.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 24..175
e-value: 5.9E-20
score: 73.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 25..173
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 213..529
e-value: 7.2E-106
score: 356.2
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 8..527
NoneNo IPR availablePANTHERPTHR31707:SF249PECTINESTERASE/PECTINESTERASE INHIBITORcoord: 8..527
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 28..173
e-value: 2.78671E-26
score: 102.516
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 217..528

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C023253.1MELO3C023253.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity