MELO3C023086.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C023086.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Description9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic
Locationchr05: 7339023 .. 7340729 (-)
RNA-Seq ExpressionMELO3C023086.jh1
SyntenyMELO3C023086.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAATCAGTTTACTATCGCCGACCCCGCCGCTGACACCTTGCCGGAGCTCCTCCACCATTGAAACCCCCCCAATTAGGAAGCTCAAAGTAATCACTCCGCCACACCCACCACTGTCCCTGCCAAAAAAGGTGTCGCCGCCGCTGCGTCAGCTTAACCCATTACAGAATTTTGCGGCATCGCTACTGGACGCCGTCGAGTCCTCACTGTTCGTCGATAAATTGCCGAAAACGGTTGACCCGGCGGTTCAAATTTCGGGTAATTTCTCTCCGGTTCCGGAATGCTCAGTCCGCCACGACCTTGAAATCATCGGAAATTTACCTGCATGCTTACGTGGCGTTTATTTCCGAAACGGCGCTAATCCCATGCATGCACCCACTGGTGGGCACCACTTGTTTGACGGTGACGGAATGATCCACGCCGTTACATTTCACAGCGGTAACAAAGCTAGTTACAGCTGTCGGTTCACTCGTACAAACCGGCTCCAGTACGAAGCCGCTTTAGGCCGACCTGTTTTCCCCAAGCCGATCGGCGAGCTTCATGGTCACCAAGGACTGGCTCGCCTAGCTATCTTTTTGGCTCGAGCCGGGATCGGGATAATCGACGGCTCGAAAGGTACAGGTGTCGCCAACGCCGGTCTGGTTTATTTCAATGGTCGATTGTTAGCTCTATCAGAAGATGATCTTCCGTATCACGTACAAATCAAGGACGACGGAGATCTTGAAACGATCGGACGGTTCGACTTCAACGGACAGATTAACTGTCCCATGATTGCTCATCCTAAAGTGGACCCCATTTCGGGAGATCTTCACGGGTTGAGTTACAACATGATTAAAAAACCTTATTTGAAATACTTACGGTTCGACATGTTCGGGAAGAAATCTCGTGACGTGGACATCACCCTTCGTGAGCCGACGATGATACACGATTTCGCAATAACGGAAAACCACGTCGTGATACCGGACCATCAAGTGGTTTTCAAGTTATTCGAAATGGTTCGGGGTGGATCACCCGTGGTGTTTGACCCCAAGAAAACTTCTCGGTTCGGTATATTACCGAAATCCGGAGTTGACGAGAAGGGGATTGTTTGGATTGAGGTGCCCAATTGCTTTTGCTTCCATTTGTGGAATGCATGGGAGGAAACTGGTGGCAATGACGAGAAAAGTATCGTTGTTATCGGGTCGTGTATGAACCCGCCTGATTCGATTTTCAACGACCGGGACCAGCCATTAAGGATCGAGCTGACGGAAATTCGGATGGATGTGAAGAACCGGAAAGTGACCCGGCGGGTGTTCGGTTCAGGGATGAATTTGGAAGCGGGTCAAATGAACCGAAGGTTTGTTGGGCGGAAAACTCGGTTCGTTTATATGGCGATAGCGGAGCCTTGGCCCAAATGTTCTGGCATTGCGAAGGTGGATTTGGAAACCGGGAAGGTGAAGAAGTTTCTATACGGTGAAGGTCGGTATGGCGGGGAACCGTTTTACGTGTCGGAAAATGGGAATTGTGGGGGAGATAATGAGAACGAGGACGGAGGGTTTATTGTAGGGTTTGTGAGGGACGAGAAGCGGGAGCGGTCTGAAGTGGTGGTGGTGAAGGCGGCGGAGATGGAGGAAGTGGCGGCGGTGAGGCTGCCTGTGAGGGTTCCGTACGGATTTCACGGCACATTTGTTAGTGAAGAAGAGTTGAATGGGCAAGCTCGCAATTAA

mRNA sequence

ATGCAAATCAGTTTACTATCGCCGACCCCGCCGCTGACACCTTGCCGGAGCTCCTCCACCATTGAAACCCCCCCAATTAGGAAGCTCAAAGTAATCACTCCGCCACACCCACCACTGTCCCTGCCAAAAAAGGTGTCGCCGCCGCTGCGTCAGCTTAACCCATTACAGAATTTTGCGGCATCGCTACTGGACGCCGTCGAGTCCTCACTGTTCGTCGATAAATTGCCGAAAACGGTTGACCCGGCGGTTCAAATTTCGGGTAATTTCTCTCCGGTTCCGGAATGCTCAGTCCGCCACGACCTTGAAATCATCGGAAATTTACCTGCATGCTTACGTGGCGTTTATTTCCGAAACGGCGCTAATCCCATGCATGCACCCACTGGTGGGCACCACTTGTTTGACGGTGACGGAATGATCCACGCCGTTACATTTCACAGCGGTAACAAAGCTAGTTACAGCTGTCGGTTCACTCGTACAAACCGGCTCCAGTACGAAGCCGCTTTAGGCCGACCTGTTTTCCCCAAGCCGATCGGCGAGCTTCATGGTCACCAAGGACTGGCTCGCCTAGCTATCTTTTTGGCTCGAGCCGGGATCGGGATAATCGACGGCTCGAAAGGTACAGGTGTCGCCAACGCCGGTCTGGTTTATTTCAATGGTCGATTGTTAGCTCTATCAGAAGATGATCTTCCGTATCACGTACAAATCAAGGACGACGGAGATCTTGAAACGATCGGACGGTTCGACTTCAACGGACAGATTAACTGTCCCATGATTGCTCATCCTAAAGTGGACCCCATTTCGGGAGATCTTCACGGGTTGAGTTACAACATGATTAAAAAACCTTATTTGAAATACTTACGGTTCGACATGTTCGGGAAGAAATCTCGTGACGTGGACATCACCCTTCGTGAGCCGACGATGATACACGATTTCGCAATAACGGAAAACCACGTCGTGATACCGGACCATCAAGTGGTTTTCAAGTTATTCGAAATGGTTCGGGGTGGATCACCCGTGGTGTTTGACCCCAAGAAAACTTCTCGGTTCGGTATATTACCGAAATCCGGAGTTGACGAGAAGGGGATTGTTTGGATTGAGGTGCCCAATTGCTTTTGCTTCCATTTGTGGAATGCATGGGAGGAAACTGGTGGCAATGACGAGAAAAGTATCGTTGTTATCGGGTCGTGTATGAACCCGCCTGATTCGATTTTCAACGACCGGGACCAGCCATTAAGGATCGAGCTGACGGAAATTCGGATGGATGTGAAGAACCGGAAAGTGACCCGGCGGGTGTTCGGTTCAGGGATGAATTTGGAAGCGGGTCAAATGAACCGAAGGTTTGTTGGGCGGAAAACTCGGTTCGTTTATATGGCGATAGCGGAGCCTTGGCCCAAATGTTCTGGCATTGCGAAGGTGGATTTGGAAACCGGGAAGGTGAAGAAGTTTCTATACGGTGAAGGTCGGTATGGCGGGGAACCGTTTTACGTGTCGGAAAATGGGAATTGTGGGGGAGATAATGAGAACGAGGACGGAGGGTTTATTGTAGGGTTTGTGAGGGACGAGAAGCGGGAGCGGTCTGAAGTGGTGGTGGTGAAGGCGGCGGAGATGGAGGAAGTGGCGGCGGTGAGGCTGCCTGTGAGGGTTCCGTACGGATTTCACGGCACATTTGTTAGTGAAGAAGAGTTGAATGGGCAAGCTCGCAATTAA

Coding sequence (CDS)

ATGCAAATCAGTTTACTATCGCCGACCCCGCCGCTGACACCTTGCCGGAGCTCCTCCACCATTGAAACCCCCCCAATTAGGAAGCTCAAAGTAATCACTCCGCCACACCCACCACTGTCCCTGCCAAAAAAGGTGTCGCCGCCGCTGCGTCAGCTTAACCCATTACAGAATTTTGCGGCATCGCTACTGGACGCCGTCGAGTCCTCACTGTTCGTCGATAAATTGCCGAAAACGGTTGACCCGGCGGTTCAAATTTCGGGTAATTTCTCTCCGGTTCCGGAATGCTCAGTCCGCCACGACCTTGAAATCATCGGAAATTTACCTGCATGCTTACGTGGCGTTTATTTCCGAAACGGCGCTAATCCCATGCATGCACCCACTGGTGGGCACCACTTGTTTGACGGTGACGGAATGATCCACGCCGTTACATTTCACAGCGGTAACAAAGCTAGTTACAGCTGTCGGTTCACTCGTACAAACCGGCTCCAGTACGAAGCCGCTTTAGGCCGACCTGTTTTCCCCAAGCCGATCGGCGAGCTTCATGGTCACCAAGGACTGGCTCGCCTAGCTATCTTTTTGGCTCGAGCCGGGATCGGGATAATCGACGGCTCGAAAGGTACAGGTGTCGCCAACGCCGGTCTGGTTTATTTCAATGGTCGATTGTTAGCTCTATCAGAAGATGATCTTCCGTATCACGTACAAATCAAGGACGACGGAGATCTTGAAACGATCGGACGGTTCGACTTCAACGGACAGATTAACTGTCCCATGATTGCTCATCCTAAAGTGGACCCCATTTCGGGAGATCTTCACGGGTTGAGTTACAACATGATTAAAAAACCTTATTTGAAATACTTACGGTTCGACATGTTCGGGAAGAAATCTCGTGACGTGGACATCACCCTTCGTGAGCCGACGATGATACACGATTTCGCAATAACGGAAAACCACGTCGTGATACCGGACCATCAAGTGGTTTTCAAGTTATTCGAAATGGTTCGGGGTGGATCACCCGTGGTGTTTGACCCCAAGAAAACTTCTCGGTTCGGTATATTACCGAAATCCGGAGTTGACGAGAAGGGGATTGTTTGGATTGAGGTGCCCAATTGCTTTTGCTTCCATTTGTGGAATGCATGGGAGGAAACTGGTGGCAATGACGAGAAAAGTATCGTTGTTATCGGGTCGTGTATGAACCCGCCTGATTCGATTTTCAACGACCGGGACCAGCCATTAAGGATCGAGCTGACGGAAATTCGGATGGATGTGAAGAACCGGAAAGTGACCCGGCGGGTGTTCGGTTCAGGGATGAATTTGGAAGCGGGTCAAATGAACCGAAGGTTTGTTGGGCGGAAAACTCGGTTCGTTTATATGGCGATAGCGGAGCCTTGGCCCAAATGTTCTGGCATTGCGAAGGTGGATTTGGAAACCGGGAAGGTGAAGAAGTTTCTATACGGTGAAGGTCGGTATGGCGGGGAACCGTTTTACGTGTCGGAAAATGGGAATTGTGGGGGAGATAATGAGAACGAGGACGGAGGGTTTATTGTAGGGTTTGTGAGGGACGAGAAGCGGGAGCGGTCTGAAGTGGTGGTGGTGAAGGCGGCGGAGATGGAGGAAGTGGCGGCGGTGAGGCTGCCTGTGAGGGTTCCGTACGGATTTCACGGCACATTTGTTAGTGAAGAAGAGTTGAATGGGCAAGCTCGCAATTAA

Protein sequence

MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAASLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIGELHGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRMDVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQARN
Homology
BLAST of MELO3C023086.jh1 vs. NCBI nr
Match: XP_008460725.1 (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucumis melo] >KAA0032007.1 9-cis-epoxycarotenoid dioxygenase NCED6 [Cucumis melo var. makuwa] >TYK16828.1 9-cis-epoxycarotenoid dioxygenase NCED6 [Cucumis melo var. makuwa])

HSP 1 Score: 1150 bits (2976), Expect = 0.0
Identity = 565/568 (99.47%), Postives = 566/568 (99.65%), Query Frame = 0

Query: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA 60
           MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA
Sbjct: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA 60

Query: 61  SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRNGA 120
           SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVY RNGA
Sbjct: 61  SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYLRNGA 120

Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIGEL 180
           NPMHAPTGGHHLFDGDGMIHAVTFHS NKASYSCRFTRTNRLQ+EAALGRPVFPKPIGEL
Sbjct: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSNNKASYSCRFTRTNRLQHEAALGRPVFPKPIGEL 180

Query: 181 HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
           HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240

Query: 241 LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI 300
           LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI
Sbjct: 241 LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI 300

Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
           TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK
Sbjct: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360

Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM 420
           GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM 420

Query: 421 DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480
           DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480

Query: 481 KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA 540
           KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA
Sbjct: 481 KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA 540

Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568
           AVRLPVRVPYGFHGTFVSEEELNGQARN
Sbjct: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568

BLAST of MELO3C023086.jh1 vs. NCBI nr
Match: XP_004150345.1 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucumis sativus] >KGN61528.1 hypothetical protein Csa_006609 [Cucumis sativus])

HSP 1 Score: 1104 bits (2855), Expect = 0.0
Identity = 543/568 (95.60%), Postives = 552/568 (97.18%), Query Frame = 0

Query: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA 60
           MQISL SP PPLT CRSSSTIETPPIRKLKVITPP PPLSLPKKVSPP R+LNPLQ FAA
Sbjct: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA 60

Query: 61  SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRNGA 120
           SLLDAVESSLFVDKLPKT+DPAVQISGNFSPVPECSVRHDLEIIGNLPACLRG+Y RNGA
Sbjct: 61  SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120

Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIGEL 180
           NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQ EAALGR VFPKPIGEL
Sbjct: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180

Query: 181 HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
           HGHQGLARLAIFLARAGIG+IDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240

Query: 241 LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI 300
           LETIGRF+FNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFD FGKKSRDVDI
Sbjct: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300

Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
           TLREPTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDPKKTSRFGILPKSGVDEK
Sbjct: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360

Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM 420
           GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVV+GSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420

Query: 421 DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480
           DVK+RKVTRRVFGSGMNLEAGQMNR  VGRKTRFVYMAIA+PWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480

Query: 481 KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA 540
           KFLYGEGRYGGEPFYV ENGN GGDNE EDGG+IVGFVRDEKRERSEVVVVKAAEMEEVA
Sbjct: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540

Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568
           AVRLPVRVPYGFHGTFVSEEELNGQARN
Sbjct: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568

BLAST of MELO3C023086.jh1 vs. NCBI nr
Match: XP_038875554.1 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Benincasa hispida])

HSP 1 Score: 1065 bits (2753), Expect = 0.0
Identity = 522/568 (91.90%), Postives = 538/568 (94.72%), Query Frame = 0

Query: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA 60
           MQI L SP P  + CRSSSTIETPPIRKLKVITPP PPLSLPK VSPPLR+LNP QN AA
Sbjct: 1   MQIYLQSPPPLPSLCRSSSTIETPPIRKLKVITPPPPPLSLPKTVSPPLRRLNPFQNLAA 60

Query: 61  SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRNGA 120
           SLLDAVESSL VDKLPKTVDP VQISGNFSPVPEC VRHDLEIIGNLPACLRGVY RNGA
Sbjct: 61  SLLDAVESSLIVDKLPKTVDPTVQISGNFSPVPECPVRHDLEIIGNLPACLRGVYLRNGA 120

Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIGEL 180
           NPMHAP GGHHLFDGDGMIHAVTFH GNKASYSCRFTRT+RLQ EAALGRPVFPKPIGEL
Sbjct: 121 NPMHAPIGGHHLFDGDGMIHAVTFHCGNKASYSCRFTRTSRLQQEAALGRPVFPKPIGEL 180

Query: 181 HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
           HGHQGLARLAIFLARAGIG+IDGSKGTGV NAGLVYFNGRLLALSEDDLPYHV+IKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVTNAGLVYFNGRLLALSEDDLPYHVKIKDDGD 240

Query: 241 LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI 300
           LETIGRF+FNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFD FGKKSRDVDI
Sbjct: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300

Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
           TL EPTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDP K SRFG+LPKS VDEK
Sbjct: 301 TLPEPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKISRFGVLPKSEVDEK 360

Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM 420
           GIVW+EVPNCFCFHLWNAWEETG NDEK++VV+GSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWMEVPNCFCFHLWNAWEETGDNDEKTVVVLGSCMNPPDSIFNDRDQPLRIELTEIRM 420

Query: 421 DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480
           DVKNRKVTRRVFGSGMNLEAGQ+NR  VGR+TRFVYMAIAEPWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKNRKVTRRVFGSGMNLEAGQVNRGLVGRRTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480

Query: 481 KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA 540
           KFLYG+GRYGGEPFYVSENGNCGGDNENEDGGF+VGFVRDEKRERSE+VVVKAAEMEEVA
Sbjct: 481 KFLYGDGRYGGEPFYVSENGNCGGDNENEDGGFVVGFVRDEKRERSELVVVKAAEMEEVA 540

Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568
           AVRLP RVPYGFHGTFVSEEEL GQA N
Sbjct: 541 AVRLPARVPYGFHGTFVSEEELKGQAPN 568

BLAST of MELO3C023086.jh1 vs. NCBI nr
Match: KAG6574873.1 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013444.1 9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 996 bits (2575), Expect = 0.0
Identity = 495/570 (86.84%), Postives = 523/570 (91.75%), Query Frame = 0

Query: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVIT--PPHPPLSLPKKV--SPPLRQLNPLQ 60
           MQISL SP P    CRSSST+ET PIRKLK++T  PP PPL  P KV  SP  R+LNP Q
Sbjct: 1   MQISLQSPLPL---CRSSSTVETTPIRKLKLLTLPPPSPPLPPPPKVVLSPLPRRLNPFQ 60

Query: 61  NFAASLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYF 120
           N AASLLDAVESSL VDKLPKTVDPAVQISGNFSPVPEC VRHDLEIIGNLPACLRGVY 
Sbjct: 61  NLAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIGNLPACLRGVYL 120

Query: 121 RNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKP 180
           RNGANPMHAP GGHHLFDGDGMIHAVTFH GN+ASYSCRFTRT+RL+ E ALGRPVFPKP
Sbjct: 121 RNGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLEQEVALGRPVFPKP 180

Query: 181 IGELHGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIK 240
           IGELHGHQGLARLAIFLARAGIG+ID SKGTGVANAGLVYFNGRLLALSEDDLPYHV+I+
Sbjct: 181 IGELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALSEDDLPYHVKIR 240

Query: 241 DDGDLETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSR 300
           D GDLETIGRF FN QI+CPMIAHPKVDP+SGDLHGLSYNMIKKPYLKYLRFD  GKK R
Sbjct: 241 DGGDLETIGRFSFNSQISCPMIAHPKVDPVSGDLHGLSYNMIKKPYLKYLRFDRLGKKVR 300

Query: 301 DVDITLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSG 360
           DVDITLR+PTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDP KTSRFGILPKSG
Sbjct: 301 DVDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILPKSG 360

Query: 361 VDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELT 420
           VDE GIVWI+VP+CFCFHLWNAWEETG N EK++V +GSCMNPPDSIFNDRDQ L IELT
Sbjct: 361 VDETGIVWIDVPDCFCFHLWNAWEETGTNGEKTVVAVGSCMNPPDSIFNDRDQALTIELT 420

Query: 421 EIRMDVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLET 480
           EIRMD++NRKVTRRVFGSGMNLEAGQ+NR+ VGR+TRFVYMAIAEPWPKCSGIAKVDLET
Sbjct: 421 EIRMDMRNRKVTRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPKCSGIAKVDLET 480

Query: 481 GKVKKFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEM 540
           GKVKKFLYG+GRYGGEPFYVSENGN G D ENEDGG++VGFVRDEKRERSE+VVVKAAEM
Sbjct: 481 GKVKKFLYGDGRYGGEPFYVSENGNSGED-ENEDGGYLVGFVRDEKRERSELVVVKAAEM 540

Query: 541 EEVAAVRLPVRVPYGFHGTFVSEEELNGQA 566
           +EVAAVRLP RVPYGFHGTFVSEEEL GQA
Sbjct: 541 KEVAAVRLPARVPYGFHGTFVSEEELKGQA 566

BLAST of MELO3C023086.jh1 vs. NCBI nr
Match: XP_023548938.1 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 994 bits (2571), Expect = 0.0
Identity = 497/573 (86.74%), Postives = 524/573 (91.45%), Query Frame = 0

Query: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVIT--PPHPPLSLP--KKVSPPL-RQLNPL 60
           MQISL SP P    CRSSST+ETPPIRKLK++T  PP PP S P  K V  PL R+LNP 
Sbjct: 1   MQISLQSPLPL---CRSSSTVETPPIRKLKLLTLPPPSPPPSPPPPKVVLSPLPRRLNPF 60

Query: 61  QNFAASLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVY 120
           QN AASLLDAVESSL VDKLPKTVDPAVQISGNFSPVPEC VRHDLEIIGNLPACLRGVY
Sbjct: 61  QNLAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIGNLPACLRGVY 120

Query: 121 FRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPK 180
            RNGANPMHAP GGHHLFDGDGMIHAVTFH GN+ASYSCRFTRT+RL+ E ALGRPVFPK
Sbjct: 121 LRNGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLEQEVALGRPVFPK 180

Query: 181 PIGELHGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQI 240
           PIGELHGHQGLARLAIFLARAGIG+ID SKGTGVANAGLVYFNGRLLALSEDDLPYHV+I
Sbjct: 181 PIGELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALSEDDLPYHVKI 240

Query: 241 KDDGDLETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKS 300
           +D GDLETIGRF FN QI+CPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFD  GKK 
Sbjct: 241 RDGGDLETIGRFSFNSQISCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRLGKKV 300

Query: 301 RDVDITLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKS 360
           RDVDITLR+PTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDP KTSRFGILPKS
Sbjct: 301 RDVDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILPKS 360

Query: 361 GVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIEL 420
           GVDE GIVWI+VP+CFCFHLWNAWEETG N EK++V +GSCMNPPDSIFNDRDQ L IEL
Sbjct: 361 GVDETGIVWIDVPDCFCFHLWNAWEETGINGEKTVVAVGSCMNPPDSIFNDRDQALTIEL 420

Query: 421 TEIRMDVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLE 480
           TEIRMD++NRK TRRVFGSGMNLEAGQ+NR+ VGR+TRFVYMAIAEPWPKCSGIAKVDLE
Sbjct: 421 TEIRMDMRNRKATRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPKCSGIAKVDLE 480

Query: 481 TGKVKKFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAE 540
           TGKVKKFLYG+GRYGGEPFYVSENGN G D ENEDGG++VGFVRDEKRERSE+VVVKAAE
Sbjct: 481 TGKVKKFLYGDGRYGGEPFYVSENGNSGED-ENEDGGYVVGFVRDEKRERSELVVVKAAE 540

Query: 541 MEEVAAVRLPVRVPYGFHGTFVSEEELNGQARN 568
           M+EVAAVRLP RVPYGFHGTFVSEEEL GQA N
Sbjct: 541 MKEVAAVRLPARVPYGFHGTFVSEEELKGQAPN 569

BLAST of MELO3C023086.jh1 vs. ExPASy Swiss-Prot
Match: Q9LRM7 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED6 PE=2 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 1.4e-200
Identity = 334/547 (61.06%), Postives = 426/547 (77.88%), Query Frame = 0

Query: 26  IRKLKVITPPH--PPLSLPKKVSPPLRQLNPLQNFAASLLDAVESSLFVD-----KLPKT 85
           I   K+ T P    P+  P K+ P    LN LQ  AA++LD +ESS+ +       LPK 
Sbjct: 35  INPFKIPTLPDLTSPVPSPVKLKPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKP 94

Query: 86  VDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRNGANPMHAPTGGHHLFDGDGM 145
            DPAVQ+SGNF+PV EC V++ LE++G +P+CL+GVY RNGANPM  P  GHHLFDGDGM
Sbjct: 95  TDPAVQLSGNFAPVNECPVQNGLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGM 154

Query: 146 IHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIGELHGHQGLARLAIFLARAGI 205
           IHAV+    N+ SYSCR+T+TNRL  E ALGR VFPKPIGELHGH GLARLA+F ARAGI
Sbjct: 155 IHAVSIGFDNQVSYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGI 214

Query: 206 GIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFDFNGQINCPMI 265
           G++DG++G GVANAG+V+FNGRLLA+SEDDLPY V+I   GDLETIGRF F+ QI+  +I
Sbjct: 215 GLVDGTRGMGVANAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVI 274

Query: 266 AHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDITLREPTMIHDFAITENHV 325
           AHPKVD  +GDLH LSYN++KKP+L+YL+F+  GKK+RDV+ITL EPTMIHDFAITEN V
Sbjct: 275 AHPKVDATTGDLHTLSYNVLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFV 334

Query: 326 VIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNA 385
           VIPD Q+VFKL EM+RGGSPV++  +K +RFG+L K  +    I W++VP+CFCFHLWNA
Sbjct: 335 VIPDQQMVFKLSEMIRGGSPVIYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNA 394

Query: 386 WEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRMDVKNRKVTRRVFGSGMNL 445
           WEE     +  IVVIGSCM+PPD+IF++  +P R+EL+EIR++++ ++  R+V  +G+NL
Sbjct: 395 WEERTEEGDPVIVVIGSCMSPPDTIFSESGEPTRVELSEIRLNMRTKESNRKVIVTGVNL 454

Query: 446 EAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVSE 505
           EAG +NR +VGRK++FVY+AIA+PWPKCSGIAKVD++ G V +F YG  R+GGEP +V E
Sbjct: 455 EAGHINRSYVGRKSQFVYIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPE 514

Query: 506 NGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVS 565
                G+ E ED G+++GFVRDE+++ SE VVV A +M++VAAVRLP RVPYGFHGTFVS
Sbjct: 515 -----GEGE-EDKGYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVS 574

BLAST of MELO3C023086.jh1 vs. ExPASy Swiss-Prot
Match: O24592 (9-cis-epoxycarotenoid dioxygenase 1, chloroplastic OS=Zea mays OX=4577 GN=VP14 PE=1 SV=2)

HSP 1 Score: 647.5 bits (1669), Expect = 1.4e-184
Identity = 315/555 (56.76%), Postives = 394/555 (70.99%), Query Frame = 0

Query: 21  IETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAASLLDAVESSLFVD------K 80
           +  P  +   +  P H   + P+K     +QLN  Q  AA+ LDA E     +       
Sbjct: 55  LPAPSRKPAAIAVPGH--AAAPRKAEGGKKQLNLFQRAAAAALDAFEEGFVANVLERPHG 114

Query: 81  LPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRNGANPMHAPTGGHHLFD 140
           LP T DPAVQI+GNF+PV E    H+L + G +P  + GVY RNGANP   P  GHHLFD
Sbjct: 115 LPSTADPAVQIAGNFAPVGERPPVHELPVSGRIPPFIDGVYARNGANPCFDPVAGHHLFD 174

Query: 141 GDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIGELHGHQGLARLAIFLA 200
           GDGM+HA+   +G   SY+CRFT T RL+ E A+GRPVFPK IGELHGH G+ARLA+F A
Sbjct: 175 GDGMVHALRIRNGAAESYACRFTETARLRQERAIGRPVFPKAIGELHGHSGIARLALFYA 234

Query: 201 RAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFDFNGQIN 260
           RA  G++D S GTGVANAGLVYFNGRLLA+SEDDLPYHV++ DDGDLET+GR+DF+GQ+ 
Sbjct: 235 RAACGLVDPSAGTGVANAGLVYFNGRLLAMSEDDLPYHVRVADDGDLETVGRYDFDGQLG 294

Query: 261 CPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDITLREPTMIHDFAIT 320
           C MIAHPK+DP +G+LH LSY++IK+PYLKY  F   G KS DV+I L +PTMIHDFAIT
Sbjct: 295 CAMIAHPKLDPATGELHALSYDVIKRPYLKYFYFRPDGTKSDDVEIPLEQPTMIHDFAIT 354

Query: 321 ENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFH 380
           EN VV+PDHQVVFKL EM+RGGSPVV D +KTSRFG+LPK   D   + W++VP+CFCFH
Sbjct: 355 ENLVVVPDHQVVFKLQEMLRGGSPVVLDKEKTSRFGVLPKHAADASEMAWVDVPDCFCFH 414

Query: 381 LWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRMDVKNRKVTRRVF-- 440
           LWNAWE+    +   +VVIGSCM P DSIFN+ D+ L   LTEIR+D +  + TRR    
Sbjct: 415 LWNAWEDEATGE---VVVIGSCMTPADSIFNESDERLESVLTEIRLDARTGRSTRRAVLP 474

Query: 441 -GSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVKKFLYGEGRYGG 500
               +NLE G +NR  +GR+TR+ Y+A+AEPWPK SG AKVDL TG++ KF YGEGR+GG
Sbjct: 475 PSQQVNLEVGMVNRNLLGRETRYAYLAVAEPWPKVSGFAKVDLSTGELTKFEYGEGRFGG 534

Query: 501 EPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYG 560
           EP +V  +         ED G+++ FV DE+   SE++VV AA+M   A V+LP RVP+G
Sbjct: 535 EPCFVPMD-PAAAHPRGEDDGYVLTFVHDERAGTSELLVVNAADMRLEATVQLPSRVPFG 594

Query: 561 FHGTFVSEEELNGQA 567
           FHGTF++ +EL  QA
Sbjct: 595 FHGTFITGQELEAQA 603

BLAST of MELO3C023086.jh1 vs. ExPASy Swiss-Prot
Match: Q5MBR5 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED3 PE=2 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 1.2e-183
Identity = 312/526 (59.32%), Postives = 381/526 (72.43%), Query Frame = 0

Query: 50  RQLNPLQNFAASLLDAVESSLFVD------KLPKTVDPAVQISGNFSPVPECSVRHDLEI 109
           ++LN  Q  AA  LDA E     +       LP T DPAVQI+GNF+PV E      L +
Sbjct: 87  KRLNFFQRAAAMALDAFEEGFVANVLERPHGLPSTADPAVQIAGNFAPVGETPPARALPV 146

Query: 110 IGNLPACLRGVYFRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQ 169
            G +P  + GVY RNGANP   P  GHHLFDGDGM+HAV   +G   SY+CRFT T RL+
Sbjct: 147 SGRIPPFINGVYARNGANPHFDPVAGHHLFDGDGMVHAVRIRNGAAESYACRFTETARLR 206

Query: 170 YEAALGRPVFPKPIGELHGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLA 229
            E A+GRP+FPK IGELHGH G+ARLA+F ARA  G++D S GTGVANAGL+YFNGRLLA
Sbjct: 207 QERAMGRPMFPKAIGELHGHSGIARLALFYARAACGLLDPSHGTGVANAGLIYFNGRLLA 266

Query: 230 LSEDDLPYHVQIKDDGDLETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYL 289
           +SEDDLPY V++  DGDLET+GR+DF+GQ+ C MIAHPK+DP +G+LH LSY++IKKPYL
Sbjct: 267 MSEDDLPYQVRVTADGDLETVGRYDFDGQLGCAMIAHPKLDPATGELHALSYDVIKKPYL 326

Query: 290 KYLRFDMFGKKSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDP 349
           KY  F   G KS DV+I L +PTMIHDFAITEN+VV+PDHQVVFKL EM+RGGSPVV D 
Sbjct: 327 KYFYFAPDGTKSADVEIPLDQPTMIHDFAITENYVVVPDHQVVFKLQEMLRGGSPVVLDK 386

Query: 350 KKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSI 409
           +KTSRFG+LPK   D   +VW++VP+CFCFHLWNAWEE    D   +VVIGSCM P DSI
Sbjct: 387 EKTSRFGVLPKHAADASEMVWVDVPDCFCFHLWNAWEEA---DTDEVVVIGSCMTPADSI 446

Query: 410 FNDRDQPLRIELTEIRMDVKNRKVTRRVF---GSGMNLEAGQMNRRFVGRKTRFVYMAIA 469
           FN+ D  L   LTEIR++ +  + TRR      S +NLE G +NR  +GRKTR+ Y+A+A
Sbjct: 447 FNESDDRLESVLTEIRLNTRTGESTRRAILPPSSQVNLEVGMVNRNLLGRKTRYAYLAVA 506

Query: 470 EPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRD 529
           EPWPK SG AKVDL TG++ KF YGEGR+GGEP +V  +         ED G+I+ FV D
Sbjct: 507 EPWPKVSGFAKVDLATGELTKFEYGEGRFGGEPCFVPMDA-AAATPRGEDDGYILSFVHD 566

Query: 530 EKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQA 567
           E+   SE++VV AA+M   A V+LP RVPYGFHGTF++ +EL  QA
Sbjct: 567 ERAGTSELLVVNAADMRLEATVQLPSRVPYGFHGTFITGDELTTQA 608

BLAST of MELO3C023086.jh1 vs. ExPASy Swiss-Prot
Match: Q9M9F5 (9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED9 PE=2 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.5e-183
Identity = 320/571 (56.04%), Postives = 408/571 (71.45%), Query Frame = 0

Query: 12  LTPCRSSSTIETPPIRKLKVITPPHPPLSLPK----------KVSPPLRQLNPLQNFAAS 71
           + PC  SS++         + +P   P S  K          K +    +LNP+Q  AA 
Sbjct: 95  VVPCYVSSSVNKKSSVSSSLQSPTFKPPSWKKLCNDVTNLIPKTTNQNPKLNPVQRTAAM 154

Query: 72  LLDAVESSLFVDKL-----PKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYF 131
           +LDAVE+++   +      PKT DPAVQI+GNF PVPE  V H+L + G +P C++GVY 
Sbjct: 155 VLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYV 214

Query: 132 RNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKP 191
           RNGANP+H P  GHHLFDGDGM+HAV F +G+  SY+CRFT TNRL  E   GRPVFPK 
Sbjct: 215 RNGANPLHKPVSGHHLFDGDGMVHAVRFDNGS-VSYACRFTETNRLVQERECGRPVFPKA 274

Query: 192 IGELHGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIK 251
           IGELHGH G+A+L +F  R   G++D + G GVANAGLVYFNG LLA+SEDDLPYHV++ 
Sbjct: 275 IGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVT 334

Query: 252 DDGDLETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSR 311
             GDLET GR+DF+GQ+   MIAHPK+DP + +L  LSY+++ KPYLKY RF   G+KS 
Sbjct: 335 QTGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSP 394

Query: 312 DVDITLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSG 371
           DV+I L +PTMIHDFAITEN VVIPD QVVF+L EM+RGGSPVV+D KK SRFGIL K+ 
Sbjct: 395 DVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMIRGGSPVVYDEKKKSRFGILNKNA 454

Query: 372 VDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELT 431
            D   I WIEVP+CFCFHLWN+WEE    +   +VVIGSCM PPDSIFN+ D+ L+  L+
Sbjct: 455 KDASSIQWIEVPDCFCFHLWNSWEEP---ETDEVVVIGSCMTPPDSIFNEHDETLQSVLS 514

Query: 432 EIRMDVKNRKVTRR-VFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLE 491
           EIR+++K  + TRR V    +NLEAG +NR  +GRKTR+ Y+A+ EPWPK SG AKVDL 
Sbjct: 515 EIRLNLKTGESTRRPVISEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLS 574

Query: 492 TGKVKKFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAE 551
           TG+++K++YGEG+YGGEP ++       GD E EDGG+I+ FV DE++ +SE+ ++ A  
Sbjct: 575 TGEIRKYIYGEGKYGGEPLFLP-----SGDGE-EDGGYIMVFVHDEEKVKSELQLINAVN 634

Query: 552 MEEVAAVRLPVRVPYGFHGTFVSEEELNGQA 567
           M+  A V LP RVPYGFHGTF+S+E+L+ QA
Sbjct: 635 MKLEATVTLPSRVPYGFHGTFISKEDLSKQA 655

BLAST of MELO3C023086.jh1 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 2.0e-183
Identity = 322/557 (57.81%), Postives = 397/557 (71.27%), Query Frame = 0

Query: 18  SSTIETPPIRKLKVITPPHPPLSL-PKKVSPPLRQLNPLQNFAASLLDAVESSLF----V 77
           SS + TPP       +   P + + PK      +Q+N  Q  AA+ LDA E  L     +
Sbjct: 51  SSALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKL 110

Query: 78  DKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRNGANPMHAPTGGHHL 137
             LPKT DP+VQI+GNF+PV E  VR +L ++G LP  ++GVY RNGANP+H P  GHH 
Sbjct: 111 HPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHF 170

Query: 138 FDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIGELHGHQGLARLAIF 197
           FDGDGM+HAV F  G+ ASY+CRFT+TNR   E  LGRPVFPK IGELHGH G+ARL +F
Sbjct: 171 FDGDGMVHAVKFEHGS-ASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLF 230

Query: 198 LARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFDFNGQ 257
            ARA  GI+D + GTGVANAGLVYFNGRLLA+SEDDLPY VQI  +GDL+T+GRFDF+GQ
Sbjct: 231 YARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQ 290

Query: 258 INCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDITLREPTMIHDFA 317
           +   MIAHPKVDP SG+L  LSY+++ KPYLKY RF   G KS DV+I L +PTM+HDFA
Sbjct: 291 LESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFA 350

Query: 318 ITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFC 377
           ITEN VV+PD QVVFKL EM+RGGSPVV+D  K +RFGIL K   D   I WI+ P+CFC
Sbjct: 351 ITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFC 410

Query: 378 FHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRMDVKNRKVTRRVF 437
           FHLWNAWEE    +   +VVIGSCM PPDSIFN+ D+ L+  L+EIR+++K  + TRR  
Sbjct: 411 FHLWNAWEEP---ETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPI 470

Query: 438 GSG----MNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVKKFLYGEGR 497
            S     +NLEAG +NR  +GRKT+F Y+A+AEPWPK SG AKVDL TG+VKK LYG+ R
Sbjct: 471 ISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNR 530

Query: 498 YGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRV 557
           YGGEP ++       G+   ED G+I+ FV DEK  +SE+ +V A  +E  A V+LP RV
Sbjct: 531 YGGEPLFLP------GEGGEEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRV 590

Query: 558 PYGFHGTFVSEEELNGQ 566
           PYGFHGTF+  ++L  Q
Sbjct: 591 PYGFHGTFIGADDLAKQ 597

BLAST of MELO3C023086.jh1 vs. ExPASy TrEMBL
Match: A0A5D3CZW1 (9-cis-epoxycarotenoid dioxygenase NCED6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold96G001030 PE=3 SV=1)

HSP 1 Score: 1150 bits (2976), Expect = 0.0
Identity = 565/568 (99.47%), Postives = 566/568 (99.65%), Query Frame = 0

Query: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA 60
           MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA
Sbjct: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA 60

Query: 61  SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRNGA 120
           SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVY RNGA
Sbjct: 61  SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYLRNGA 120

Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIGEL 180
           NPMHAPTGGHHLFDGDGMIHAVTFHS NKASYSCRFTRTNRLQ+EAALGRPVFPKPIGEL
Sbjct: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSNNKASYSCRFTRTNRLQHEAALGRPVFPKPIGEL 180

Query: 181 HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
           HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240

Query: 241 LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI 300
           LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI
Sbjct: 241 LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI 300

Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
           TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK
Sbjct: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360

Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM 420
           GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM 420

Query: 421 DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480
           DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480

Query: 481 KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA 540
           KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA
Sbjct: 481 KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA 540

Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568
           AVRLPVRVPYGFHGTFVSEEELNGQARN
Sbjct: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568

BLAST of MELO3C023086.jh1 vs. ExPASy TrEMBL
Match: A0A1S3CD31 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103499487 PE=3 SV=1)

HSP 1 Score: 1150 bits (2976), Expect = 0.0
Identity = 565/568 (99.47%), Postives = 566/568 (99.65%), Query Frame = 0

Query: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA 60
           MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA
Sbjct: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA 60

Query: 61  SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRNGA 120
           SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVY RNGA
Sbjct: 61  SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYLRNGA 120

Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIGEL 180
           NPMHAPTGGHHLFDGDGMIHAVTFHS NKASYSCRFTRTNRLQ+EAALGRPVFPKPIGEL
Sbjct: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSNNKASYSCRFTRTNRLQHEAALGRPVFPKPIGEL 180

Query: 181 HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
           HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240

Query: 241 LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI 300
           LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI
Sbjct: 241 LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI 300

Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
           TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK
Sbjct: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360

Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM 420
           GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM 420

Query: 421 DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480
           DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480

Query: 481 KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA 540
           KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA
Sbjct: 481 KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA 540

Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568
           AVRLPVRVPYGFHGTFVSEEELNGQARN
Sbjct: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568

BLAST of MELO3C023086.jh1 vs. ExPASy TrEMBL
Match: A0A0A0LIE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G160620 PE=3 SV=1)

HSP 1 Score: 1104 bits (2855), Expect = 0.0
Identity = 543/568 (95.60%), Postives = 552/568 (97.18%), Query Frame = 0

Query: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA 60
           MQISL SP PPLT CRSSSTIETPPIRKLKVITPP PPLSLPKKVSPP R+LNPLQ FAA
Sbjct: 1   MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA 60

Query: 61  SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRNGA 120
           SLLDAVESSLFVDKLPKT+DPAVQISGNFSPVPECSVRHDLEIIGNLPACLRG+Y RNGA
Sbjct: 61  SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120

Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIGEL 180
           NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQ EAALGR VFPKPIGEL
Sbjct: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180

Query: 181 HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
           HGHQGLARLAIFLARAGIG+IDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240

Query: 241 LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI 300
           LETIGRF+FNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFD FGKKSRDVDI
Sbjct: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300

Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
           TLREPTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDPKKTSRFGILPKSGVDEK
Sbjct: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360

Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM 420
           GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVV+GSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420

Query: 421 DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480
           DVK+RKVTRRVFGSGMNLEAGQMNR  VGRKTRFVYMAIA+PWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480

Query: 481 KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA 540
           KFLYGEGRYGGEPFYV ENGN GGDNE EDGG+IVGFVRDEKRERSEVVVVKAAEMEEVA
Sbjct: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540

Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568
           AVRLPVRVPYGFHGTFVSEEELNGQARN
Sbjct: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568

BLAST of MELO3C023086.jh1 vs. ExPASy TrEMBL
Match: A0A6J1H4F0 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111460311 PE=3 SV=1)

HSP 1 Score: 992 bits (2565), Expect = 0.0
Identity = 493/571 (86.34%), Postives = 520/571 (91.07%), Query Frame = 0

Query: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVIT--PPHPPLSLPKKVSPPL-RQLNPLQN 60
           MQISL SP P    CRSSST+ETPPIRKLK++T  PP PP   PK V  PL R+LNP QN
Sbjct: 1   MQISLQSPLPL---CRSSSTVETPPIRKLKLLTLPPPSPPPPPPKVVLSPLPRRLNPFQN 60

Query: 61  FAASLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFR 120
            AASLLDAVESSL VDKLPKTVDPAVQISGNFSPVPEC VRHDLEIIGNLPACLRGVY R
Sbjct: 61  LAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIGNLPACLRGVYLR 120

Query: 121 NGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPI 180
           NGANPMHAP GGHHLFDGDGMIHAVTFH GN+ASYSCRFTRT+RL+ E ALGRPVFPKPI
Sbjct: 121 NGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLEQEVALGRPVFPKPI 180

Query: 181 GELHGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKD 240
           GELHGHQGLARLAIFLARAGIG+ID SKGTGVANAGLVYFNGRLLALSEDDLPYHV I+D
Sbjct: 181 GELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALSEDDLPYHVLIRD 240

Query: 241 DGDLETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRD 300
            GDLETIGRF FN QI+CPMIAHPKVDP+SGDLHGLSYNMIKKPYLKYLRFD  G+K RD
Sbjct: 241 GGDLETIGRFSFNSQISCPMIAHPKVDPVSGDLHGLSYNMIKKPYLKYLRFDRLGRKVRD 300

Query: 301 VDITLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGV 360
           VDITLR+PTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDP KTSRFGILPKSGV
Sbjct: 301 VDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILPKSGV 360

Query: 361 DEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTE 420
           DE GIVWI+VP+CFCFHLWNAWEETG N EK++V +GSCMNPPDSIFNDRDQ L IELTE
Sbjct: 361 DETGIVWIDVPDCFCFHLWNAWEETGSNGEKTVVAVGSCMNPPDSIFNDRDQALTIELTE 420

Query: 421 IRMDVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETG 480
           IRMD++NRK TRRVFGSGMNLEAGQ+NR+ VGR+TRFVYMAIAEPWPKCSGIAKVDLET 
Sbjct: 421 IRMDMRNRKATRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPKCSGIAKVDLETR 480

Query: 481 KVKKFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEME 540
           KVKKFLYG+GRYGGEPFYVSENGN G D ENEDGG++VGFVRDEKRERSE+VVVKAAEM 
Sbjct: 481 KVKKFLYGDGRYGGEPFYVSENGNSGED-ENEDGGYVVGFVRDEKRERSELVVVKAAEMM 540

Query: 541 EVAAVRLPVRVPYGFHGTFVSEEELNGQARN 568
           EVAAVRLP RVPYGFHGTFVSEEEL GQA N
Sbjct: 541 EVAAVRLPARVPYGFHGTFVSEEELKGQAPN 567

BLAST of MELO3C023086.jh1 vs. ExPASy TrEMBL
Match: A0A6J1L4D2 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499033 PE=3 SV=1)

HSP 1 Score: 988 bits (2554), Expect = 0.0
Identity = 491/571 (85.99%), Postives = 520/571 (91.07%), Query Frame = 0

Query: 1   MQISLLSPTPPLTPCRSSSTIETPPIRKLKVIT--PPHPPLSLPKKVSPPL-RQLNPLQN 60
           MQISL SP P    CRSSST+ETPPIRKLK++T  PP P  S PK VS PL  ++NP QN
Sbjct: 1   MQISLQSPLPL---CRSSSTVETPPIRKLKLLTLPPPSPSPSPPKVVSTPLPHRINPFQN 60

Query: 61  FAASLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFR 120
            AASLLDAVESSL VDKLPKTVDPAVQISGNFSPVPEC VRHDLEIIGNLPACLRGVY R
Sbjct: 61  LAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIGNLPACLRGVYLR 120

Query: 121 NGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPI 180
           NGANPMHAP GGHHLFDGDGMIHAVTFH GN+ASYSCRFTRT+RL  E ALGRPVFPKPI
Sbjct: 121 NGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLVQEVALGRPVFPKPI 180

Query: 181 GELHGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKD 240
           GELHGHQGLARLAIFLARAGIG+ID SKGTGVANAGLVYFNGRLLALSEDDLPYHV+I+D
Sbjct: 181 GELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALSEDDLPYHVKIRD 240

Query: 241 DGDLETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRD 300
            GDLETIGRF FN QI+CPMIAHPKVDPI GDLHGLSYNMIKKPYLKYLRFD  GKK RD
Sbjct: 241 GGDLETIGRFSFNSQISCPMIAHPKVDPILGDLHGLSYNMIKKPYLKYLRFDRLGKKVRD 300

Query: 301 VDITLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGV 360
           VDITLR+PTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFD  KTSRFGILPKSGV
Sbjct: 301 VDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDSNKTSRFGILPKSGV 360

Query: 361 DEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTE 420
           DE GIVWI+VP+CFCFHLWNAWEETG N EK++V +GSCMNPPDSIFNDRDQ L IELTE
Sbjct: 361 DETGIVWIDVPDCFCFHLWNAWEETGSNGEKTVVAVGSCMNPPDSIFNDRDQALTIELTE 420

Query: 421 IRMDVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETG 480
           IRMD++NRK TRRVFGSGMNLEAGQ+NR+ VGR+TRFVYMAIAEPWPKCSGIAKVDLETG
Sbjct: 421 IRMDMRNRKATRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPKCSGIAKVDLETG 480

Query: 481 KVKKFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEME 540
           KVKKFLYG+GR+GGEPFYVSENGN G D ENEDGG++VGFVRDEKR+RSE+VVVKAAEM+
Sbjct: 481 KVKKFLYGDGRFGGEPFYVSENGNSGED-ENEDGGYVVGFVRDEKRQRSELVVVKAAEMK 540

Query: 541 EVAAVRLPVRVPYGFHGTFVSEEELNGQARN 568
           EVAAVRLP RVPYGFHGTFVSEEEL GQA N
Sbjct: 541 EVAAVRLPARVPYGFHGTFVSEEELKGQAPN 567

BLAST of MELO3C023086.jh1 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 700.7 bits (1807), Expect = 9.9e-202
Identity = 334/547 (61.06%), Postives = 426/547 (77.88%), Query Frame = 0

Query: 26  IRKLKVITPPH--PPLSLPKKVSPPLRQLNPLQNFAASLLDAVESSLFVD-----KLPKT 85
           I   K+ T P    P+  P K+ P    LN LQ  AA++LD +ESS+ +       LPK 
Sbjct: 35  INPFKIPTLPDLTSPVPSPVKLKPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKP 94

Query: 86  VDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRNGANPMHAPTGGHHLFDGDGM 145
            DPAVQ+SGNF+PV EC V++ LE++G +P+CL+GVY RNGANPM  P  GHHLFDGDGM
Sbjct: 95  TDPAVQLSGNFAPVNECPVQNGLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGM 154

Query: 146 IHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIGELHGHQGLARLAIFLARAGI 205
           IHAV+    N+ SYSCR+T+TNRL  E ALGR VFPKPIGELHGH GLARLA+F ARAGI
Sbjct: 155 IHAVSIGFDNQVSYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGI 214

Query: 206 GIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFDFNGQINCPMI 265
           G++DG++G GVANAG+V+FNGRLLA+SEDDLPY V+I   GDLETIGRF F+ QI+  +I
Sbjct: 215 GLVDGTRGMGVANAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVI 274

Query: 266 AHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDITLREPTMIHDFAITENHV 325
           AHPKVD  +GDLH LSYN++KKP+L+YL+F+  GKK+RDV+ITL EPTMIHDFAITEN V
Sbjct: 275 AHPKVDATTGDLHTLSYNVLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFV 334

Query: 326 VIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNA 385
           VIPD Q+VFKL EM+RGGSPV++  +K +RFG+L K  +    I W++VP+CFCFHLWNA
Sbjct: 335 VIPDQQMVFKLSEMIRGGSPVIYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNA 394

Query: 386 WEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRMDVKNRKVTRRVFGSGMNL 445
           WEE     +  IVVIGSCM+PPD+IF++  +P R+EL+EIR++++ ++  R+V  +G+NL
Sbjct: 395 WEERTEEGDPVIVVIGSCMSPPDTIFSESGEPTRVELSEIRLNMRTKESNRKVIVTGVNL 454

Query: 446 EAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVSE 505
           EAG +NR +VGRK++FVY+AIA+PWPKCSGIAKVD++ G V +F YG  R+GGEP +V E
Sbjct: 455 EAGHINRSYVGRKSQFVYIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPE 514

Query: 506 NGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVS 565
                G+ E ED G+++GFVRDE+++ SE VVV A +M++VAAVRLP RVPYGFHGTFVS
Sbjct: 515 -----GEGE-EDKGYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVS 574

BLAST of MELO3C023086.jh1 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 644.0 bits (1660), Expect = 1.1e-184
Identity = 320/571 (56.04%), Postives = 408/571 (71.45%), Query Frame = 0

Query: 12  LTPCRSSSTIETPPIRKLKVITPPHPPLSLPK----------KVSPPLRQLNPLQNFAAS 71
           + PC  SS++         + +P   P S  K          K +    +LNP+Q  AA 
Sbjct: 95  VVPCYVSSSVNKKSSVSSSLQSPTFKPPSWKKLCNDVTNLIPKTTNQNPKLNPVQRTAAM 154

Query: 72  LLDAVESSLFVDKL-----PKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYF 131
           +LDAVE+++   +      PKT DPAVQI+GNF PVPE  V H+L + G +P C++GVY 
Sbjct: 155 VLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYV 214

Query: 132 RNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKP 191
           RNGANP+H P  GHHLFDGDGM+HAV F +G+  SY+CRFT TNRL  E   GRPVFPK 
Sbjct: 215 RNGANPLHKPVSGHHLFDGDGMVHAVRFDNGS-VSYACRFTETNRLVQERECGRPVFPKA 274

Query: 192 IGELHGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIK 251
           IGELHGH G+A+L +F  R   G++D + G GVANAGLVYFNG LLA+SEDDLPYHV++ 
Sbjct: 275 IGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVT 334

Query: 252 DDGDLETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSR 311
             GDLET GR+DF+GQ+   MIAHPK+DP + +L  LSY+++ KPYLKY RF   G+KS 
Sbjct: 335 QTGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSP 394

Query: 312 DVDITLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSG 371
           DV+I L +PTMIHDFAITEN VVIPD QVVF+L EM+RGGSPVV+D KK SRFGIL K+ 
Sbjct: 395 DVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMIRGGSPVVYDEKKKSRFGILNKNA 454

Query: 372 VDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELT 431
            D   I WIEVP+CFCFHLWN+WEE    +   +VVIGSCM PPDSIFN+ D+ L+  L+
Sbjct: 455 KDASSIQWIEVPDCFCFHLWNSWEEP---ETDEVVVIGSCMTPPDSIFNEHDETLQSVLS 514

Query: 432 EIRMDVKNRKVTRR-VFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLE 491
           EIR+++K  + TRR V    +NLEAG +NR  +GRKTR+ Y+A+ EPWPK SG AKVDL 
Sbjct: 515 EIRLNLKTGESTRRPVISEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLS 574

Query: 492 TGKVKKFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAE 551
           TG+++K++YGEG+YGGEP ++       GD E EDGG+I+ FV DE++ +SE+ ++ A  
Sbjct: 575 TGEIRKYIYGEGKYGGEPLFLP-----SGDGE-EDGGYIMVFVHDEEKVKSELQLINAVN 634

Query: 552 MEEVAAVRLPVRVPYGFHGTFVSEEELNGQA 567
           M+  A V LP RVPYGFHGTF+S+E+L+ QA
Sbjct: 635 MKLEATVTLPSRVPYGFHGTFISKEDLSKQA 655

BLAST of MELO3C023086.jh1 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 643.7 bits (1659), Expect = 1.4e-184
Identity = 322/557 (57.81%), Postives = 397/557 (71.27%), Query Frame = 0

Query: 18  SSTIETPPIRKLKVITPPHPPLSL-PKKVSPPLRQLNPLQNFAASLLDAVESSLF----V 77
           SS + TPP       +   P + + PK      +Q+N  Q  AA+ LDA E  L     +
Sbjct: 51  SSALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKL 110

Query: 78  DKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRNGANPMHAPTGGHHL 137
             LPKT DP+VQI+GNF+PV E  VR +L ++G LP  ++GVY RNGANP+H P  GHH 
Sbjct: 111 HPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHF 170

Query: 138 FDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIGELHGHQGLARLAIF 197
           FDGDGM+HAV F  G+ ASY+CRFT+TNR   E  LGRPVFPK IGELHGH G+ARL +F
Sbjct: 171 FDGDGMVHAVKFEHGS-ASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLF 230

Query: 198 LARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFDFNGQ 257
            ARA  GI+D + GTGVANAGLVYFNGRLLA+SEDDLPY VQI  +GDL+T+GRFDF+GQ
Sbjct: 231 YARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQ 290

Query: 258 INCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDITLREPTMIHDFA 317
           +   MIAHPKVDP SG+L  LSY+++ KPYLKY RF   G KS DV+I L +PTM+HDFA
Sbjct: 291 LESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFA 350

Query: 318 ITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFC 377
           ITEN VV+PD QVVFKL EM+RGGSPVV+D  K +RFGIL K   D   I WI+ P+CFC
Sbjct: 351 ITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFC 410

Query: 378 FHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRMDVKNRKVTRRVF 437
           FHLWNAWEE    +   +VVIGSCM PPDSIFN+ D+ L+  L+EIR+++K  + TRR  
Sbjct: 411 FHLWNAWEEP---ETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPI 470

Query: 438 GSG----MNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVKKFLYGEGR 497
            S     +NLEAG +NR  +GRKT+F Y+A+AEPWPK SG AKVDL TG+VKK LYG+ R
Sbjct: 471 ISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNR 530

Query: 498 YGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRV 557
           YGGEP ++       G+   ED G+I+ FV DEK  +SE+ +V A  +E  A V+LP RV
Sbjct: 531 YGGEPLFLP------GEGGEEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRV 590

Query: 558 PYGFHGTFVSEEELNGQ 566
           PYGFHGTF+  ++L  Q
Sbjct: 591 PYGFHGTFIGADDLAKQ 597

BLAST of MELO3C023086.jh1 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 620.9 bits (1600), Expect = 9.9e-178
Identity = 310/551 (56.26%), Postives = 391/551 (70.96%), Query Frame = 0

Query: 21  IETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAASLLDAVESSLFVDK----LP 80
           I+   + K + +  P    + P+    PLR LN  Q  AA  +DA E +L   +    LP
Sbjct: 41  IDVTELTKKRQLFTPRTTATPPQ--HNPLR-LNIFQKAAAIAIDAAERALISHEQDSPLP 100

Query: 81  KTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRNGANPMHAPTGGHHLFDGD 140
           KT DP VQI+GN+SPVPE SVR +L + G +P C+ GVY RNGANPM  PT GHHLFDGD
Sbjct: 101 KTADPRVQIAGNYSPVPESSVRRNLTVEGTIPDCIDGVYIRNGANPMFEPTAGHHLFDGD 160

Query: 141 GMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIGELHGHQGLARLAIFLARA 200
           GM+HAV   +G+ ASY+CRFT+T RL  E  LGRPVFPK IGELHGH G+ARL +F AR 
Sbjct: 161 GMVHAVKITNGS-ASYACRFTKTERLVQEKRLGRPVFPKAIGELHGHSGIARLMLFYARG 220

Query: 201 GIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFDFNGQINCP 260
             G+I+   G GVANAGLVYFN RLLA+SEDDLPY ++I   GDL+T+GR+DF+GQ+   
Sbjct: 221 LCGLINNQNGVGVANAGLVYFNNRLLAMSEDDLPYQLKITQTGDLQTVGRYDFDGQLKSA 280

Query: 261 MIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDITLREPTMIHDFAITEN 320
           MIAHPK+DP++ +LH LSY+++KKPYLKY RF   G KS +++I L  PTMIHDFAITEN
Sbjct: 281 MIAHPKLDPVTKELHALSYDVVKKPYLKYFRFSPDGVKSPELEIPLETPTMIHDFAITEN 340

Query: 321 HVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLW 380
            VVIPD QVVFKL EM+ G SPVVFD +K SR GI+PK   +   I+W+  P  FCFHLW
Sbjct: 341 FVVIPDQQVVFKLGEMISGKSPVVFDGEKVSRLGIMPKDATEASQIIWVNSPETFCFHLW 400

Query: 381 NAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRMDVKNRKVTRR--VFGS 440
           NAWE     + + IVVIGSCM+P DSIFN+RD+ LR  L+EIR++++ RK TRR  +   
Sbjct: 401 NAWESP---ETEEIVVIGSCMSPADSIFNERDESLRSVLSEIRINLRTRKTTRRSLLVNE 460

Query: 441 GMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPF 500
            +NLE G +NR  +GRKTRF ++AIA PWPK SG AKVDL TG++KK++YG  +YGGEPF
Sbjct: 461 DVNLEIGMVNRNRLGRKTRFAFLAIAYPWPKVSGFAKVDLCTGEMKKYIYGGEKYGGEPF 520

Query: 501 YVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHG 560
           ++   GN G   ENED G+I   V DE+ + SE+ ++ A  ++  A ++LP RVPYGFHG
Sbjct: 521 FLP--GNSGNGEENEDDGYIFCHVHDEETKTSELQIINAVNLKLEATIKLPSRVPYGFHG 580

Query: 561 TFVSEEELNGQ 566
           TFV   EL  Q
Sbjct: 581 TFVDSNELVDQ 582

BLAST of MELO3C023086.jh1 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 611.7 bits (1576), Expect = 6.0e-175
Identity = 310/571 (54.29%), Postives = 395/571 (69.18%), Query Frame = 0

Query: 3   ISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAASL 62
           +S  +  P      ++S  +TP +        P+P   +P+K      + NPLQ  A++ 
Sbjct: 32  VSFTNTKPRRRKLSANSVSDTPNLLNFPNYPSPNP--IIPEK---DTSRWNPLQRAASAA 91

Query: 63  LDAVESSLF----VDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYFRN 122
           LD  E++L        LPKTVDP  QISGN++PVPE SV+  L + G +P C+ GVY RN
Sbjct: 92  LDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRN 151

Query: 123 GANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQYEAALGRPVFPKPIG 182
           GANP+  P  GHHLFDGDGM+HAV   +G+ ASYSCRFT T RL  E  LG P+FPK IG
Sbjct: 152 GANPLFEPVSGHHLFDGDGMVHAVKITNGD-ASYSCRFTETERLVQEKQLGSPIFPKAIG 211

Query: 183 ELHGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDD 242
           ELHGH G+ARL +F AR   G+++   GTGVANAGLVYF+ RLLA+SEDDLPY V++ D+
Sbjct: 212 ELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDN 271

Query: 243 GDLETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDV 302
           GDLETIGRFDF+GQ++  MIAHPK+DP++ +L  LSY+++KKPYLKY +F   G+KS DV
Sbjct: 272 GDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDV 331

Query: 303 DITLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVD 362
           +I L  PTM+HDFAITEN VVIPD QVVFKL +M  G SPV +D +K SRFGILP++  D
Sbjct: 332 EIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKD 391

Query: 363 EKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEI 422
              +VW+E P  FCFHLWNAWE     +   +VVIGSCM P DSIFN+ D+ L   L+EI
Sbjct: 392 ASEMVWVESPETFCFHLWNAWESP---ETDEVVVIGSCMTPADSIFNECDEQLNSVLSEI 451

Query: 423 RMDVKNRKVTRRVFGSG---MNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLE 482
           R+++K  K TRR    G   MNLEAG +NR  +GRKTR+ Y+AIAEPWPK SG AKVDL 
Sbjct: 452 RLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLS 511

Query: 483 TGKVKKFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAE 542
           TG+VK   YG  +YGGEPF++      G +++ ED G+I+ FV DE+   SE+ +V A  
Sbjct: 512 TGEVKNHFYGGKKYGGEPFFLPR----GLESDGEDDGYIMSFVHDEESWESELHIVNAVT 571

Query: 543 MEEVAAVRLPVRVPYGFHGTFVSEEELNGQA 567
           +E  A V+LP RVPYGFHGTFV+  ++  QA
Sbjct: 572 LELEATVKLPSRVPYGFHGTFVNSADMLNQA 589

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008460725.10.099.47PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucumis melo]... [more]
XP_004150345.10.095.609-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucumis sativus] >KGN615... [more]
XP_038875554.10.091.909-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Benincasa hispida][more]
KAG6574873.10.086.849-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic, partial [Cucurbita argyr... [more]
XP_023548938.10.086.749-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucurbita pepo subsp. pe... [more]
Match NameE-valueIdentityDescription
Q9LRM71.4e-20061.069-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Arabidopsis thaliana O... [more]
O245921.4e-18456.769-cis-epoxycarotenoid dioxygenase 1, chloroplastic OS=Zea mays OX=4577 GN=VP14 P... [more]
Q5MBR51.2e-18359.329-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q9M9F51.5e-18356.049-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9LRR72.0e-18357.819-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A5D3CZW10.099.479-cis-epoxycarotenoid dioxygenase NCED6 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3CD310.099.479-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucumis melo OX=3656 G... [more]
A0A0A0LIE20.095.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G160620 PE=3 SV=1[more]
A0A6J1H4F00.086.349-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucurbita moschata OX=... [more]
A0A6J1L4D20.085.999-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
AT3G24220.19.9e-20261.06nine-cis-epoxycarotenoid dioxygenase 6 [more]
AT1G78390.11.1e-18456.04nine-cis-epoxycarotenoid dioxygenase 9 [more]
AT3G14440.11.4e-18457.81nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT4G18350.19.9e-17856.26nine-cis-epoxycarotenoid dioxygenase 2 [more]
AT1G30100.16.0e-17554.29nine-cis-epoxycarotenoid dioxygenase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 94..558
e-value: 4.9E-114
score: 382.0
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 48..565
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR10543:SF1019-CIS-EPOXYCAROTENOID DIOXYGENASE NCED6, CHLOROPLASTICcoord: 48..565

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C023086.jh1.t1MELO3C023086.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen