MELO3C022770.jh1 (gene) Melon (Harukei-3) v1.41

BLAST of MELO3C022770.jh1 vs. NCBI nr
Match: TYK21080.1 (4-coumarate--CoA ligase-like 6 [Cucumis melo var. makuwa])

HSP 1 Score: 1125 bits (2909), Expect = 0.0
Identity = 577/578 (99.83%), Postives = 577/578 (99.83%), Query Frame = 0

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQ 540
           GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGS LSQKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

Query: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 578
           VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPASKL
Sbjct: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 578

BLAST of MELO3C022770.jh1 vs. NCBI nr
Match: XP_004144410.1 (4-coumarate--CoA ligase-like 6 [Cucumis sativus] >KGN58405.1 hypothetical protein Csa_001031 [Cucumis sativus])

HSP 1 Score: 1065 bits (2755), Expect = 0.0
Identity = 545/578 (94.29%), Postives = 557/578 (96.37%), Query Frame = 0

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MARL++N QFSFETPENEA ITKKIP SERTNKYPNWYSPDTGIC SVHGYRKLPSDPFL
Sbjct: 1   MARLINN-QFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIF+PIIILGALYLGAV+TTMFPQSSSSEIKKRITDCNVRLAF ITQKIKNFEALGIKT
Sbjct: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNF+LMR MGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV RFRSLKQVSCGAAASSKKTID+F
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           V AL HVDFIQGYGMTESTA+GTRGFN K  RNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQ 540
           GFQIAPTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGS L+QKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQ 540

Query: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 578
           VAPYKKVRKVIFTESIPKSAAGKVLRRELQKH PASKL
Sbjct: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 577

BLAST of MELO3C022770.jh1 vs. NCBI nr
Match: XP_008460452.1 (PREDICTED: 4-coumarate--CoA ligase-like 6 [Cucumis melo])

HSP 1 Score: 1057 bits (2733), Expect = 0.0
Identity = 542/552 (98.19%), Postives = 545/552 (98.73%), Query Frame = 0

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQ 540
           GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGS LSQKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

Query: 541 VAPYKKVRKVIF 552
           V+   ++   IF
Sbjct: 541 VSHIFQICPCIF 552

BLAST of MELO3C022770.jh1 vs. NCBI nr
Match: KAA0068160.1 (4-coumarate--CoA ligase-like 6 [Cucumis melo var. makuwa])

HSP 1 Score: 1054 bits (2726), Expect = 0.0
Identity = 539/540 (99.81%), Postives = 539/540 (99.81%), Query Frame = 0

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQ 540
           GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGS LSQKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

BLAST of MELO3C022770.jh1 vs. NCBI nr
Match: XP_038888724.1 (4-coumarate--CoA ligase-like 6 [Benincasa hispida])

HSP 1 Score: 988 bits (2555), Expect = 0.0
Identity = 502/575 (87.30%), Postives = 538/575 (93.57%), Query Frame = 0

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MA LL ND FS++TPENE  ITKKIP SE+TNKYPNWYSPDTGIC SVH  R +PSDPFL
Sbjct: 1   MAPLL-NDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFL 60

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS  F+HNG+SA+IDS TG+SISYKE+F MVKSMASGL   GISQGDVVLL+LP
Sbjct: 61  DVVSFIFS--FKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPII+LG LYLGAV+TTMFPQSSSSEIKKRI DCNVRLAFTITQKIKNFE+LGI+ 
Sbjct: 121 NSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQA 180

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPE TNFDLM+PMGFS F+ELISSGSDL +RPVIRQEDTAA+LFSSGTTGVSKGVML+
Sbjct: 181 IGVPEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLT 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPT+NVYLAAIPMFHIYGLSIFVMGL+SLGSSVV+MSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           DVK+VVKAIDRFKVTHFPVVPPI+ A++RTAEKIGV RFRSLKQVSCGAA+ +KKTIDDF
Sbjct: 301 DVKEVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDF 360

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           V+A  HVDFIQGYGMTESTAIGTRGFN K  RNY SVGLLAPNTEAKVVDWV+GS MPPG
Sbjct: 361 VKAFPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEATMSTID+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQ 540
           GFQIAPTDLEAVV+ HPEVLDTAV AAKDEECGEIPVAFVVKKPGS LSQKDV+DYVAQQ
Sbjct: 481 GFQIAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQ 540

Query: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPA 575
           VAPYKKVRKV+FTESIPKSAAGKVLRREL KHLP+
Sbjct: 541 VAPYKKVRKVVFTESIPKSAAGKVLRRELGKHLPS 572

BLAST of MELO3C022770.jh1 vs. ExPASy Swiss-Prot
Match: Q84P24 (4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=2)

HSP 1 Score: 647.9 bits (1670), Expect = 1.1e-184
Identity = 323/543 (59.48%), Postives = 424/543 (78.08%), Query Frame = 0

Query: 35  PNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELF 94
           P W+S  TGI  S      LP DP LD VS +FS   +H+G +ALIDSLTG SIS+ EL 
Sbjct: 22  PFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFS--HKHHGDTALIDSLTGFSISHTELQ 81

Query: 95  PMVKSMASGL-HNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKK 154
            MV+SMA+G+ H LG+ QGDVV L+LPNS+++P+I L  + LGA++TTM P SS  EIKK
Sbjct: 82  IMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKK 141

Query: 155 RITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYELISSGSDLIKR 214
           ++++C+V LAFT T+ ++   +LG+  I V E+ +FD +R +    FY ++      + +
Sbjct: 142 QVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSIMKESFGFVPK 201

Query: 215 PVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAIP 274
           P+I+Q+D AAI++SSGTTG SKGV+L+HRN I+++ELFVRFEASQYEY  + NVYLAA+P
Sbjct: 202 PLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALP 261

Query: 275 MFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRTAEK 334
           + HIYGLS+FVMGL+SLGS++VVM +FD  DVV  I+RFK+THFPVVPP+L+AL++ A+ 
Sbjct: 262 LCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAKG 321

Query: 335 IGVRRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNKKTRN 394
           +    F+SLKQVS GAA  S+K I+DF+Q L HVD IQGYGMTESTA+GTRGFN++K   
Sbjct: 322 VCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGFNSEKLSR 381

Query: 395 YLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQENWL 454
           Y SVGLLAPN +AKVVDW +GS +PPG  GEL ++GPG MKGYLNNP+AT  +I +++WL
Sbjct: 382 YSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWL 441

Query: 455 HTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECG 514
            TGDI YFD DGYL++VDR+KE+IKYKGFQIAP DLEAV+++HP ++D AV AA +EECG
Sbjct: 442 RTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECG 501

Query: 515 EIPVAFVVKKPGSTLSQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRRELQKHL 574
           EIPVAFVV++  +TLS++DV+ YVA QVAPY+KVRKV+   SIPKS  GK+LR+EL++ L
Sbjct: 502 EIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKILRKELKRIL 561

Query: 575 PAS 577
             S
Sbjct: 562 TNS 561

BLAST of MELO3C022770.jh1 vs. ExPASy Swiss-Prot
Match: Q6YYZ2 (4-coumarate--CoA ligase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL3 PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 2.3e-142
Identity = 282/550 (51.27%), Postives = 366/550 (66.55%), Query Frame = 0

Query: 35  PNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFS-VQFQHNGHSALIDSLTGNSISYKEL 94
           P +YS  TGI  S+H    LP+DP L +V+ +F+ +     G   L+D+ T +++S  +L
Sbjct: 25  PPFYSAATGIYSSIHPPVALPTDPSLTLVAHLFARLPLADPGAPTLVDAATASAVSRADL 84

Query: 95  FPMVKSMASGLHNL-GISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIK 154
             +V S+A+GL    G+ +G VVLL+LPNS+ +P+  L  L  GAV TTM P SS +EI 
Sbjct: 85  RRLVASLAAGLRRRHGVRKGSVVLLLLPNSVAFPVSFLAVLAAGAVATTMNPSSSPAEIA 144

Query: 155 KRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMG------FSSFYELISS 214
            +       L             L    + VPE  +       G      F++F  ++  
Sbjct: 145 AQARATGACLVLASRDGAARLPPLAAPVVLVPEILDHSAAADDGDDDQRVFAAFRAMLDG 204

Query: 215 G---SDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLP 274
           G         PV+ Q+D  AIL+SSGT+G SKGV+L+HRN I+  ELFVRFEASQY    
Sbjct: 205 GGGDGTETAVPVVGQDDAVAILYSSGTSGRSKGVVLTHRNLIAMTELFVRFEASQYHARG 264

Query: 275 TK-NVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPP 334
            + NVY+AA+PM H+YGLS+F +GL+S+G++VVVM +FD  D V AI R+KVTH P+VPP
Sbjct: 265 ARENVYMAALPMSHVYGLSLFAVGLLSIGATVVVMRRFDAGDAVAAIGRYKVTHMPLVPP 324

Query: 335 ILIALSRTAEKIGV--RRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTA 394
           I+ A+ R A   GV   +  SL QVSCGAA  +   I +F+QA  HVDFIQGYGMTESTA
Sbjct: 325 IMAAMVRAAAAGGVPPSQVASLVQVSCGAAPITAALIHEFLQAFPHVDFIQGYGMTESTA 384

Query: 395 IGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNP 454
           +GTRGFN  K + Y SVGLLAPN  AK+V   + S +PPG +GEL L GPG MKGYL++ 
Sbjct: 385 VGTRGFNTSKHKKYTSVGLLAPNMHAKIVHLESSSCLPPGFSGELWLHGPGIMKGYLSDD 444

Query: 455 EATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVL 514
           +   +   ++ WL TGDI YFD DGYLY+V RLK+ IKYKGFQIAP DLE V+I HPE+L
Sbjct: 445 DDACTR--KDGWLRTGDIAYFDLDGYLYIVGRLKDTIKYKGFQIAPGDLEEVLIHHPEIL 504

Query: 515 DTAVAAAKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQVAPYKKVRKVIFTESIPKSA 571
           D AV +A+DEE GEIPVAFVV++ GS LS K V++YVA+QVAPYK+VRKV+F E+IPKS 
Sbjct: 505 DVAVTSAEDEEAGEIPVAFVVRRSGSNLSCKQVMEYVAKQVAPYKRVRKVVFVEAIPKSP 564

BLAST of MELO3C022770.jh1 vs. ExPASy Swiss-Prot
Match: Q336M7 (4-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL2 PE=3 SV=3)

HSP 1 Score: 455.3 bits (1170), Expect = 1.0e-126
Identity = 257/515 (49.90%), Postives = 337/515 (65.44%), Query Frame = 0

Query: 36  NWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFP 95
           ++YS  TG+  S+H    LPSDP L +V  +FS    H+ HS L+D+ T  ++S  +   
Sbjct: 14  SFYSAATGLYSSLHPPLPLPSDPSLSLVPHLFSHLPLHH-HSLLVDAPTAATLSCADFRR 73

Query: 96  MVKSMASGL-HNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKR 155
           +V S+A+GL   L I++G +VLL+LPNS+ +P+  L  L  GAV TTM P S+ +EI  R
Sbjct: 74  LVSSLAAGLRRRLHIARGSLVLLLLPNSLTFPVAFLAVLATGAVATTMNPSSAPAEIAAR 133

Query: 156 ITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYELISSGSDL-IKR 215
           + D    L    T        L    + VP+              F+ L+ +  +  ++ 
Sbjct: 134 LRDTAPSLVLASTHNAAKLPPLAAPLVLVPDTFQQQHDDDQFDFFFHALLETDPETPVEM 193

Query: 216 PV-IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAI 275
            V + Q+D AA+L+SSGT+G SKGV+++HRN I+ +ELFVRFEASQY      NVYLAA+
Sbjct: 194 GVGVGQDDAAAVLYSSGTSGRSKGVVVTHRNLIAMVELFVRFEASQYTRPARDNVYLAAL 253

Query: 276 PMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRTAE 335
           PMFH+YGLS+F +GL+SLG +VVVM +F+V D VKAI ++KVTH P+VPPI+ AL R   
Sbjct: 254 PMFHVYGLSLFAVGLLSLGCTVVVMRRFNVDDAVKAIRKYKVTHLPLVPPIMSALLRANP 313

Query: 336 KIGVRRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNKKTR 395
            +      SL QVS GAA  +   I  F+ A  HVDFIQGYGMTESTA+GTRGFN  K +
Sbjct: 314 PL---ELDSLLQVSSGAAPLNHTLIHHFLHAFPHVDFIQGYGMTESTAVGTRGFNTCKHK 373

Query: 396 NYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMK------GYLNNPEATMST 455
            Y SVGLLAPN  AK+V   +GS +PPG  GEL L GP  MK      GYLN+ +   + 
Sbjct: 374 KYASVGLLAPNMHAKIVHLESGSCLPPGSYGELWLHGPAIMKEFCFVTGYLNDDDDAFTR 433

Query: 456 IDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAA 515
             ++ WL TGDI YFD DGYL++V RLK+ IKYKGFQIAP DLEAV+I HPE++D AV +
Sbjct: 434 --KDGWLRTGDIAYFDSDGYLFIVGRLKDTIKYKGFQIAPADLEAVLIRHPEIIDVAVTS 493

Query: 516 AKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQV 542
            +DEE GEIPVAFVV+K GSTLS   V++YVA+QV
Sbjct: 494 DEDEEAGEIPVAFVVRKSGSTLSCTHVMEYVAKQV 522

BLAST of MELO3C022770.jh1 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 4.1e-123
Identity = 237/549 (43.17%), Postives = 357/549 (65.03%), Query Frame = 0

Query: 38  YSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMV 97
           Y     I +S     +LP +  +DV +FI S    H+G  A ID+ TG  +++ +L+  V
Sbjct: 15  YCKSNSIFYSKREPVQLPQNHSVDVTTFISS--RAHHGKIAFIDAATGRHLTFPQLWRAV 74

Query: 98  KSMASGLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITD 157
            S+A+ L  +GI +GDV+LL+ PNSI++P++ L  + LGA+ITT  P ++  EI K+ITD
Sbjct: 75  DSVATCLSAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAKQITD 134

Query: 158 CNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPV-- 217
               LAFTI Q +       +  + + +     L + +   S     S G  + K P   
Sbjct: 135 SKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVS-----SLGEMMRKEPSPN 194

Query: 218 -----IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLA 277
                + QEDTA +L+SSGTTG SKGV+ SH+N I+ ++  +    S++     ++ ++ 
Sbjct: 195 RIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQTIL----SRFGTEDGEHTFIC 254

Query: 278 AIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRT 337
            +PMFHIYGL+ F MGL+S GS++V++SKF++ +++ AI++++ T+ P+VPPIL+AL + 
Sbjct: 255 TVPMFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKN 314

Query: 338 AEKIGVR-RFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNK 397
           A  I  +    SL+ V  G A  SK+ I+ FV+    V  +QGYG+TEST IG      +
Sbjct: 315 ANHIRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQ 374

Query: 398 KTRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQ 457
           ++R Y + G+L+P+ EAK+V+  TG ++   +TGEL LRGP  MKGY +N EAT STID 
Sbjct: 375 ESRRYGTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDS 434

Query: 458 ENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKD 517
           E WL TGD+ Y D DG+++VVDRLKE+IKYKG+Q+AP +LEA++++HPE+ D AV    D
Sbjct: 435 EGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYPD 494

Query: 518 EECGEIPVAFVVKKPGSTLSQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRREL 577
           +E G+ P+A+VV+K GS LS+  V+D++A+ VAPYK++RKV F  SIPK+ +GK+LR++L
Sbjct: 495 KEAGQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKDL 551

Query: 578 QKHLPASKL 579
            K L  SKL
Sbjct: 555 IK-LATSKL 551

BLAST of MELO3C022770.jh1 vs. ExPASy Swiss-Prot
Match: Q84P21 (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 432.6 bits (1111), Expect = 7.2e-120
Identity = 231/522 (44.25%), Postives = 347/522 (66.48%), Query Frame = 0

Query: 54  LPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGD 113
           LP +P LDV +FI S    H G  A ID+ TG ++++ EL+  V+S+A  L  +GI +G 
Sbjct: 26  LPPNPSLDVTTFISSQ--AHRGRIAFIDASTGQNLTFTELWRAVESVADCLSEIGIRKGH 85

Query: 114 VVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNF 173
           VVLL+ PNSI +P++ L  + LGA+ITT  P ++S+EI K+I D N  LAFT +Q +   
Sbjct: 86  VVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKI 145

Query: 174 EALGIK-TIGVPENTNFDLMRPM-GFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTT 233
            A   K  I + +    D +  +       +   SG+ + +R  + Q+DTA +L+SSGTT
Sbjct: 146 SAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGNRVKER--VDQDDTATLLYSSGTT 205

Query: 234 GVSKGVMLSHRNFISTIELFV-RFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSL 293
           G+SKGV+ SHRN I+ ++  V RF +   E       ++  +PMFHIYGL+ F  GL++ 
Sbjct: 206 GMSKGVISSHRNLIAMVQTIVNRFGSDDGE-----QRFICTVPMFHIYGLAAFATGLLAY 265

Query: 294 GSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVR-RFRSLKQVSCGA 353
           GS+++V+SKF++ +++ AI +++ T  P+VPPIL+A+   A++I  +    S+  V CG 
Sbjct: 266 GSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGG 325

Query: 354 AASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVV 413
           A  SK+  + F +    V  +QGYG+TEST IG      +++R Y + G L+ + E ++V
Sbjct: 326 APLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIV 385

Query: 414 DWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYV 473
           D VTG  + P +TGEL L+GP  MKGY +N EAT ST+D E WL TGD+ Y D DG+++V
Sbjct: 386 DPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFV 445

Query: 474 VDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSTLS 533
           VDRLKE+IKYKG+Q+AP +LEA+++ HPE+ D AV    D+E G+ P+A+VV+K GS+LS
Sbjct: 446 VDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLS 505

Query: 534 QKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRRELQK 572
           +K ++++VA+QVAPYK++RKV F  SIPK+ +GK+LR++L K
Sbjct: 506 EKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLIK 538

BLAST of MELO3C022770.jh1 vs. ExPASy TrEMBL
Match: A0A5D3DC80 (4-coumarate--CoA ligase-like 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold392G00450 PE=4 SV=1)

HSP 1 Score: 1125 bits (2909), Expect = 0.0
Identity = 577/578 (99.83%), Postives = 577/578 (99.83%), Query Frame = 0

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQ 540
           GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGS LSQKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

Query: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 578
           VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPASKL
Sbjct: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 578

BLAST of MELO3C022770.jh1 vs. ExPASy TrEMBL
Match: A0A0A0L8X1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G638510 PE=4 SV=1)

HSP 1 Score: 1065 bits (2755), Expect = 0.0
Identity = 545/578 (94.29%), Postives = 557/578 (96.37%), Query Frame = 0

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MARL++N QFSFETPENEA ITKKIP SERTNKYPNWYSPDTGIC SVHGYRKLPSDPFL
Sbjct: 1   MARLINN-QFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIF+PIIILGALYLGAV+TTMFPQSSSSEIKKRITDCNVRLAF ITQKIKNFEALGIKT
Sbjct: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNF+LMR MGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV RFRSLKQVSCGAAASSKKTID+F
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           V AL HVDFIQGYGMTESTA+GTRGFN K  RNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQ 540
           GFQIAPTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGS L+QKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQ 540

Query: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 578
           VAPYKKVRKVIFTESIPKSAAGKVLRRELQKH PASKL
Sbjct: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 577

BLAST of MELO3C022770.jh1 vs. ExPASy TrEMBL
Match: A0A1S3CCH7 (4-coumarate--CoA ligase-like 6 OS=Cucumis melo OX=3656 GN=LOC103499264 PE=4 SV=1)

HSP 1 Score: 1057 bits (2733), Expect = 0.0
Identity = 542/552 (98.19%), Postives = 545/552 (98.73%), Query Frame = 0

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQ 540
           GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGS LSQKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

Query: 541 VAPYKKVRKVIF 552
           V+   ++   IF
Sbjct: 541 VSHIFQICPCIF 552

BLAST of MELO3C022770.jh1 vs. ExPASy TrEMBL
Match: A0A5A7VLT7 (4-coumarate--CoA ligase-like 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold238G001280 PE=4 SV=1)

HSP 1 Score: 1054 bits (2726), Expect = 0.0
Identity = 539/540 (99.81%), Postives = 539/540 (99.81%), Query Frame = 0

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQ 540
           GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGS LSQKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

BLAST of MELO3C022770.jh1 vs. ExPASy TrEMBL
Match: A0A6J1HGK9 (4-coumarate--CoA ligase-like 6 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464107 PE=4 SV=1)

HSP 1 Score: 939 bits (2427), Expect = 0.0
Identity = 477/575 (82.96%), Postives = 519/575 (90.26%), Query Frame = 0

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MA LL NDQFSF+TPE++   TKKIP     NKYP WYSPDTGIC SVH  R LP+DPFL
Sbjct: 1   MAPLL-NDQFSFQTPESQTVQTKKIP-----NKYPGWYSPDTGICRSVHASRDLPTDPFL 60

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DV S+I S  FQHNG SALIDS TG+SISY+EL+ MV SMASGL  LG+SQGDVVLL+LP
Sbjct: 61  DVASYILS--FQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPI++LG LYLGAVITTMFPQSSS EIKKRI++CNVRLAF   Q + NFEA G++ 
Sbjct: 121 NSIFYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEAFGVQA 180

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTN DLMRPMGFSSFYELIS G DL KRPVIRQEDTAAILFSSGTTGVSKGV+L+
Sbjct: 181 IGVPENTNLDLMRPMGFSSFYELISGGFDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLT 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFIST+ELFVRFEASQYEYL T+NVYLAAIPMFH+YGLSIFVMGL+SLGSS+VVMSKF
Sbjct: 241 HRNFISTVELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           DVK+VV AIDRFKVTHFPVVPPI+I ++RTA K G  +FRSLKQVSCGAA+ SKK I DF
Sbjct: 301 DVKEVVVAIDRFKVTHFPVVPPIMIVMARTAVKFGGHKFRSLKQVSCGAASLSKKIIGDF 360

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           VQAL HVDFIQGYGMTE+TA+GTRGFN KK +NYLSVGLLAPN EAKVVDWV+GS MPPG
Sbjct: 361 VQALPHVDFIQGYGMTETTAVGTRGFNTKKAQNYLSVGLLAPNMEAKVVDWVSGSIMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPG MKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQ 540
           GFQIAPTDLE+VVI HPEVLDTAVAAA+DEECGEIPVAFVVKKPGS+LSQKDV+DYVAQQ
Sbjct: 481 GFQIAPTDLESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQ 540

Query: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPA 575
           VAPYKK+RKV+FTESIPKSAAGKVLR+EL KHLP+
Sbjct: 541 VAPYKKIRKVVFTESIPKSAAGKVLRKELGKHLPS 567

BLAST of MELO3C022770.jh1 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 647.9 bits (1670), Expect = 7.7e-186
Identity = 323/543 (59.48%), Postives = 424/543 (78.08%), Query Frame = 0

Query: 35  PNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELF 94
           P W+S  TGI  S      LP DP LD VS +FS   +H+G +ALIDSLTG SIS+ EL 
Sbjct: 22  PFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFS--HKHHGDTALIDSLTGFSISHTELQ 81

Query: 95  PMVKSMASGL-HNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKK 154
            MV+SMA+G+ H LG+ QGDVV L+LPNS+++P+I L  + LGA++TTM P SS  EIKK
Sbjct: 82  IMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKK 141

Query: 155 RITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYELISSGSDLIKR 214
           ++++C+V LAFT T+ ++   +LG+  I V E+ +FD +R +    FY ++      + +
Sbjct: 142 QVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSIMKESFGFVPK 201

Query: 215 PVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAIP 274
           P+I+Q+D AAI++SSGTTG SKGV+L+HRN I+++ELFVRFEASQYEY  + NVYLAA+P
Sbjct: 202 PLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALP 261

Query: 275 MFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRTAEK 334
           + HIYGLS+FVMGL+SLGS++VVM +FD  DVV  I+RFK+THFPVVPP+L+AL++ A+ 
Sbjct: 262 LCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAKG 321

Query: 335 IGVRRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNKKTRN 394
           +    F+SLKQVS GAA  S+K I+DF+Q L HVD IQGYGMTESTA+GTRGFN++K   
Sbjct: 322 VCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGFNSEKLSR 381

Query: 395 YLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQENWL 454
           Y SVGLLAPN +AKVVDW +GS +PPG  GEL ++GPG MKGYLNNP+AT  +I +++WL
Sbjct: 382 YSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWL 441

Query: 455 HTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECG 514
            TGDI YFD DGYL++VDR+KE+IKYKGFQIAP DLEAV+++HP ++D AV AA +EECG
Sbjct: 442 RTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECG 501

Query: 515 EIPVAFVVKKPGSTLSQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRRELQKHL 574
           EIPVAFVV++  +TLS++DV+ YVA QVAPY+KVRKV+   SIPKS  GK+LR+EL++ L
Sbjct: 502 EIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKILRKELKRIL 561

Query: 575 PAS 577
             S
Sbjct: 562 TNS 561

BLAST of MELO3C022770.jh1 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 432.6 bits (1111), Expect = 5.1e-121
Identity = 231/522 (44.25%), Postives = 347/522 (66.48%), Query Frame = 0

Query: 54  LPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGD 113
           LP +P LDV +FI S    H G  A ID+ TG ++++ EL+  V+S+A  L  +GI +G 
Sbjct: 26  LPPNPSLDVTTFISSQ--AHRGRIAFIDASTGQNLTFTELWRAVESVADCLSEIGIRKGH 85

Query: 114 VVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNF 173
           VVLL+ PNSI +P++ L  + LGA+ITT  P ++S+EI K+I D N  LAFT +Q +   
Sbjct: 86  VVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKI 145

Query: 174 EALGIK-TIGVPENTNFDLMRPM-GFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTT 233
            A   K  I + +    D +  +       +   SG+ + +R  + Q+DTA +L+SSGTT
Sbjct: 146 SAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGNRVKER--VDQDDTATLLYSSGTT 205

Query: 234 GVSKGVMLSHRNFISTIELFV-RFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSL 293
           G+SKGV+ SHRN I+ ++  V RF +   E       ++  +PMFHIYGL+ F  GL++ 
Sbjct: 206 GMSKGVISSHRNLIAMVQTIVNRFGSDDGE-----QRFICTVPMFHIYGLAAFATGLLAY 265

Query: 294 GSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVR-RFRSLKQVSCGA 353
           GS+++V+SKF++ +++ AI +++ T  P+VPPIL+A+   A++I  +    S+  V CG 
Sbjct: 266 GSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGG 325

Query: 354 AASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVV 413
           A  SK+  + F +    V  +QGYG+TEST IG      +++R Y + G L+ + E ++V
Sbjct: 326 APLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIV 385

Query: 414 DWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYV 473
           D VTG  + P +TGEL L+GP  MKGY +N EAT ST+D E WL TGD+ Y D DG+++V
Sbjct: 386 DPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFV 445

Query: 474 VDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSTLS 533
           VDRLKE+IKYKG+Q+AP +LEA+++ HPE+ D AV    D+E G+ P+A+VV+K GS+LS
Sbjct: 446 VDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLS 505

Query: 534 QKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRRELQK 572
           +K ++++VA+QVAPYK++RKV F  SIPK+ +GK+LR++L K
Sbjct: 506 EKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLIK 538

BLAST of MELO3C022770.jh1 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 417.2 bits (1071), Expect = 2.2e-116
Identity = 227/539 (42.12%), Postives = 343/539 (63.64%), Query Frame = 0

Query: 43  GICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMAS 102
           GI  S+     LP DP   +VSF+F     +    A+ DS TG+S+++ +L   V  +A 
Sbjct: 10  GIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAH 69

Query: 103 GLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRL 162
           G H LGI + DVVL+  PNS  +P+  L    +G V TT  P  + +E+ K+I D N ++
Sbjct: 70  GFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKI 129

Query: 163 AFTITQ---KIKNFE----ALGIK-TIGVPENTNFDLMRPMGFSSFYELISSGSDLIKRP 222
             ++ Q   KIK F+     LG K T+ +P  +N  ++      SF  ++     + + P
Sbjct: 130 IISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKIL------SFDNVMELSEPVSEYP 189

Query: 223 V--IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAI 282
              I+Q DTAA+L+SSGTTG SKGV L+H NFI+   +    +    EY     V+L  +
Sbjct: 190 FVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEY---HGVFLCFL 249

Query: 283 PMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRTAE 342
           PMFH++GL++     +  G+++V M++F+++ V+K I++F+VTH  VVPP+ +ALS+ + 
Sbjct: 250 PMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI 309

Query: 343 KIGVRRF--RSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNKK 402
              V++F   SLK +  GAA   K  +++  + + +V  +QGYGMTE+  I +       
Sbjct: 310 ---VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLG 369

Query: 403 TRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQE 462
            RN  S G+LAP  EA++V   TG S PP + GE+ +RGP  MKGYLNNP+AT  TID++
Sbjct: 370 KRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKK 429

Query: 463 NWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKDE 522
           +W+HTGD+ YF+ DG LYVVDR+KE+IKYKGFQ+AP +LE ++++HP++LD  V    DE
Sbjct: 430 SWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDE 489

Query: 523 ECGEIPVAFVVKKPGSTLSQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRREL 570
           E GE+P+AFVV+ P S+++++D+  ++A+QVAPYK++R+V F   +PKSAAGK+LRREL
Sbjct: 490 EAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRREL 536

BLAST of MELO3C022770.jh1 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 416.0 bits (1068), Expect = 4.9e-116
Identity = 236/552 (42.75%), Postives = 345/552 (62.50%), Query Frame = 0

Query: 38  YSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMV 97
           +   T I +S      LP + FLDV SFI S    H G +  +D++TG  +S+ EL+  V
Sbjct: 27  FCESTSIFYSKREPMALPPNQFLDVTSFIASQ--PHRGKTVFVDAVTGRRLSFPELWLGV 86

Query: 98  KSMASGLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITD 157
           + +A  L+ LG+ +G+VV+++ PNSI +PI+ L  + LGA+ITT  P ++S EI K+I D
Sbjct: 87  ERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQIGD 146

Query: 158 CNVRLAFTITQKIK--------NFEALGIKTIGVPENTNFDLMRPMG-FSSFYELISSGS 217
               LAFT  + +         N   + +    VP  +  D ++ +G   +  E   S S
Sbjct: 147 SRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIETEPSES 206

Query: 218 DLIKRPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELF-VRFEASQYEYLPTKNV 277
            + +R  + Q+DTAA+L+SSGTTG SKGVMLSHRN I+ ++ +  RF   Q         
Sbjct: 207 RVKQR--VNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRT------- 266

Query: 278 YLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIAL 337
            +  IPM HI+G   F  GL++LG ++VV+ KFD+  ++ A++  + ++  +VPPI++A+
Sbjct: 267 -ICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAM 326

Query: 338 SRTAEKIGVR-RFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGF 397
              A +I  +    SL  V  G A  S++  + FV+    V  +QGYG+TESTAI    F
Sbjct: 327 VNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMF 386

Query: 398 NNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMST 457
           N ++T+ Y + GLLAPN E K+VD  TG  +   +TGEL +R P  MKGY  N EAT ST
Sbjct: 387 NKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATAST 446

Query: 458 IDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAA 517
           ID E WL TGD+ Y D DG+++VVDRLKE+IK  G+Q+AP +LEA+++AHPE+ D AV  
Sbjct: 447 IDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIP 506

Query: 518 AKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLR 577
             D + G+ P+A++V+K GS LS+ +++ +VA+QV+PYKK+RKV F  SIPK+ +GK+LR
Sbjct: 507 IPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILR 565

Query: 578 RELQKHLPASKL 579
           REL K L  SKL
Sbjct: 567 RELTK-LTTSKL 565

BLAST of MELO3C022770.jh1 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 399.4 bits (1025), Expect = 4.8e-111
Identity = 227/555 (40.90%), Postives = 339/555 (61.08%), Query Frame = 0

Query: 40  PDTGICHSVHGY------RKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKEL 99
           P +G C S   +        LP +   DV +FI S    H G +A ID+ TG  +++ +L
Sbjct: 12  PRSGFCKSNSTFYSKRQPLSLPPNLSRDVTTFISSQ--PHRGKTAFIDAATGQCLTFSDL 71

Query: 100 FPMVKSMASGL-HNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIK 159
           +  V  +A  L H +GI +GDVVL++ PNSIF P++ L  + LGAV TT    ++S EI 
Sbjct: 72  WRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEIS 131

Query: 160 KRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYELISSGSDLIK 219
           K+I D N  L FT T+++     + I  +   +    +L      +S   ++   S+++K
Sbjct: 132 KQIADSNPTLVFT-TRQLAPKLPVAISVVLTDDEVYQEL------TSAIRVVGILSEMVK 191

Query: 220 --------RPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPT 279
                   R  + Q+DTA +L+SSGTTG SKGV+ SHRN  + +  F+       + L  
Sbjct: 192 KEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFIS------DNLKR 251

Query: 280 KNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPIL 339
            ++++  +PMFH YGL  F MG ++LGS+VV++ +F + D++ A+++ + T   + PP+L
Sbjct: 252 DDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVL 311

Query: 340 IALSRTAEKIGVR-RFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGT 399
           +A+   A+ I  +    SLK V CG A  SK+  + F++    VD +QGY +TES   G 
Sbjct: 312 VAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGA 371

Query: 400 RGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEAT 459
              + +++R Y + G L  + EA++VD  TG  M   +TGEL L+GP   KGY  N EAT
Sbjct: 372 FTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEAT 431

Query: 460 MSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTA 519
             TI+ E WL TGD+ Y D DG+L+VVDRLKE+IKYKG+Q+ P +LEA++I HP++LD A
Sbjct: 432 NETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAA 491

Query: 520 VAAAKDEECGEIPVAFVVKKPGSTLSQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGK 579
           V    D+E G+ P+A+VV+K  S LS+K V+D++++QVAPYKK+RKV F  SIPK+A+GK
Sbjct: 492 VIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASGK 550