MELO3C022695.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C022695.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
DescriptionE3 ubiquitin-protein ligase RNF144B-like
Locationchr04: 19793914 .. 19796175 (-)
RNA-Seq ExpressionMELO3C022695.jh1
SyntenyMELO3C022695.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSstart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCACGAGTTCATAAATATAAAAAATTGGTATACCGAGTTGAATTAACAAATCTGGAAACCGATAGAAAGGAAGTTGTTAGGATTGTTTCTTTCTCCGCTGCGGGTACAGATTCAAATACAAATAACAATAAATCATCCACACTCGTTTTCCTCTCATTCATTTCCCAATGCTTTCCCTTCCCACAATCCCTTAAATTCCCCCTTTCGAATTCCCTTTCTCCACTTCAAAATTCCTTCTAATGGCCTCTCCTTCTTCCTCCATCTTCCAACACCATCTATCCGATGAAAATTACGCAGAAGAGTTACAGCTTCAAGAAGTTATCACTTCCTCCTTATTATCTCCTCCAATAAAAATCCCCATTCATCCATCCCCAACACCCCACTCTTCTACTTCTCAAGTTCTCTGTTCAATCTGTACAGACGCAAAATCCCATTCCCAAATGTTCACCAACAGAGTATGTACACATACATTCTGCACCGATTGCATCTCAAAGCACATCGCTGCAAAGCTTGAGGTTACAATGGCAGTGAAGTGTCCGGAGCCGAATTGCGGGACGGTGCTGGAGCCGGAGATGTGTGGATCCTTCGTACCGAAGAGGGTTTTGGAGCGGTGGGCGGATGCTCTGTTTGAAGCGATGATTCTGAAGTCGAAGAGATTGAATTGTCCGTTTAAGGATTGTGGGGCGGCGATGATTGATGAAGGGGATGAGGAAGGGGTGACGGCGGTGGAATGTGGGAGTTGTTGGAGGTTGTTCTGTGCAGAGTGTAGAGTGGGATGGCATGGGGAAATGAAATGTGGAGAGTTTCAGAGACTGAGGAAAGAGGCGGGTGTAAGTGGAGATAAAGATGATGCGATGACTGTGAAACTAGCTGAGAATAAGAAATGGAGGAGATGTCCTCATTGTAAAATTTACGTTGAGAAGACTGTTGGTTGTGTTCATATCCTCTGCAGGTTTGTTCTTCAATATACCCTTTACTTTTTGGTTTTGTTCTATGCTTCCAATTAATTGTCAACCTAGTTTGTGTTAAATTGTAGAATTATAAAATGAAGTTTTTCGGAAAGAGGGAATTAGTTAATAAGGCTGGTTAATAAAGTGGGAGTTAGCCTAAGTATTGATATGTGGATGGCCTTGTCATGATTCAAAGGGTAGGTTGTATGGGTGTGGTATCTGGGCTTGGGGTAGGACGAAAAACTAAGTTTTAGTTTTGACATGGGTGTGTGGAGAATTGAACCTTTCGGATGGTGCTAGTTGAGGTAGGTTGGCAAAAAATGAAGTGGTTTAAATGACAATCCAAGTAATTTTGATTGAGAAGTAGGTAAACAAAGTCTAAATTTAAATTTACTAAATAAAACTAACTTAAGCTTTGAGATGTTTACCTCAATAAAGGGGAGGTTAAGCTTGTTTAGGCCTATGATAAGATGAAGTTTTTAATTTCCTTATCCTTCTTATGCCTATGATAGAGTAAATAAACATAAGAAACACTATATTAGGATTCCTTAATTCTCTAAAATTGATGAAAATCGTAACTATAGACTTGCTTTTATTGAAGATTGAAATAAAAATATATAAGATCTAATCTTTACGAGAAATAAGTTAAACTTTATAACAAAATAAAAGTGATATTAATAGACTTTTTTTTGTATGACATCTAATATTAAAGAAACTATCGGTAAAGTGTAGAGATATTCATACTATTTTAATTCATTACTCGACATCCAAGAATTATGTAAGGTCAACATAATGAAACAAATTTATGATAGATTAATAAAGTATCTAATGAAAGAAGATATTATCTACTACAGTAACACTAAAAAAATTTTCACTTTAATAAAGGAGGACATGAGCTTGTAGCATCTTTAATTTAGCTAGGAACTAATGTGTGTCGTTATGTTAAGGTAGTGATATAATAACGATAATATTAAGGGTAGTTAAATCAAATATTAATATAATATAGAACACCATACATAAAATAACTTTGAAGATAAATAGTAAATGATAGTGTATTTGTTAAGAAGAAGATGCAATTGATGGAGTTTTTAATTGTACGAGGAAATTTGTAGGTGTGGATCGGAATTTTGCTACAGCTGTGGAGGTAAGTGGGGTGGTGCTCATGCTTGTCGGGGTTCAGCTTATGAACGCACTGTCCATCGACGTTGATTTTCAACCCATCCTCATCCATATGAATGAATTCAAAATTGTTAGAATTTCATTTTTATGTTTTAAGAGTTAGATTTTAACAACTAAAATTTTATATTTAAC

mRNA sequence

TCCACGAGTTCATAAATATAAAAAATTGGTATACCGAGTTGAATTAACAAATCTGGAAACCGATAGAAAGGAAGTTGTTAGGATTGTTTCTTTCTCCGCTGCGGGTACAGATTCAAATACAAATAACAATAAATCATCCACACTCGTTTTCCTCTCATTCATTTCCCAATGCTTTCCCTTCCCACAATCCCTTAAATTCCCCCTTTCGAATTCCCTTTCTCCACTTCAAAATTCCTTCTAATGGCCTCTCCTTCTTCCTCCATCTTCCAACACCATCTATCCGATGAAAATTACGCAGAAGAGTTACAGCTTCAAGAAGTTATCACTTCCTCCTTATTATCTCCTCCAATAAAAATCCCCATTCATCCATCCCCAACACCCCACTCTTCTACTTCTCAAGTTCTCTGTTCAATCTGTACAGACGCAAAATCCCATTCCCAAATGTTCACCAACAGAGTATGTACACATACATTCTGCACCGATTGCATCTCAAAGCACATCGCTGCAAAGCTTGAGGTTACAATGGCAGTGAAGTGTCCGGAGCCGAATTGCGGGACGGTGCTGGAGCCGGAGATGTGTGGATCCTTCGTACCGAAGAGGGTTTTGGAGCGGTGGGCGGATGCTCTGTTTGAAGCGATGATTCTGAAGTCGAAGAGATTGAATTGTCCGTTTAAGGATTGTGGGGCGGCGATGATTGATGAAGGGGATGAGGAAGGGGTGACGGCGGTGGAATGTGGGAGTTGTTGGAGGTTGTTCTGTGCAGAGTGTAGAGTGGGATGGCATGGGGAAATGAAATGTGGAGAGTTTCAGAGACTGAGGAAAGAGGCGGGTGTAAGTGGAGATAAAGATGATGCGATGACTGTGAAACTAGCTGAGAATAAGAAATGGAGGAGATGTCCTCATTGTAAAATTTACGTTGAGAAGACTGTTGGTTGTGTTCATATCCTCTGCAGGTGTGGATCGGAATTTTGCTACAGCTGTGGAGGTAAGTGGGGTGGTGCTCATGCTTGTCGGGGTTCAGCTTATGAACGCACTGTCCATCGACGTTGATTTTCAACCCATCCTCATCCATATGAATGAATTCAAAATTGTTAGAATTTCATTTTTATGTTTTAAGAGTTAGATTTTAACAACTAAAATTTTATATTTAAC

Coding sequence (CDS)

ATGGCCTCTCCTTCTTCCTCCATCTTCCAACACCATCTATCCGATGAAAATTACGCAGAAGAGTTACAGCTTCAAGAAGTTATCACTTCCTCCTTATTATCTCCTCCAATAAAAATCCCCATTCATCCATCCCCAACACCCCACTCTTCTACTTCTCAAGTTCTCTGTTCAATCTGTACAGACGCAAAATCCCATTCCCAAATGTTCACCAACAGAGTATGTACACATACATTCTGCACCGATTGCATCTCAAAGCACATCGCTGCAAAGCTTGAGGTTACAATGGCAGTGAAGTGTCCGGAGCCGAATTGCGGGACGGTGCTGGAGCCGGAGATGTGTGGATCCTTCGTACCGAAGAGGGTTTTGGAGCGGTGGGCGGATGCTCTGTTTGAAGCGATGATTCTGAAGTCGAAGAGATTGAATTGTCCGTTTAAGGATTGTGGGGCGGCGATGATTGATGAAGGGGATGAGGAAGGGGTGACGGCGGTGGAATGTGGGAGTTGTTGGAGGTTGTTCTGTGCAGAGTGTAGAGTGGGATGGCATGGGGAAATGAAATGTGGAGAGTTTCAGAGACTGAGGAAAGAGGCGGGTGTAAGTGGAGATAAAGATGATGCGATGACTGTGAAACTAGCTGAGAATAAGAAATGGAGGAGATGTCCTCATTGTAAAATTTACGTTGAGAAGACTGTTGGTTGTGTTCATATCCTCTGCAGGTGTGGATCGGAATTTTGCTACAGCTGTGGAGGTAAGTGGGGTGGTGCTCATGCTTGTCGGGGTTCAGCTTATGAACGCACTGTCCATCGACGTTGA

Protein sequence

MASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICTDAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKRVLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGWHGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCGSEFCYSCGGKWGGAHACRGSAYERTVHRR
Homology
BLAST of MELO3C022695.jh1 vs. NCBI nr
Match: XP_008460341.1 (PREDICTED: E3 ubiquitin-protein ligase RNF144B-like [Cucumis melo] >KAA0053550.1 E3 ubiquitin-protein ligase RNF144B-like protein [Cucumis melo var. makuwa] >TYK19145.1 E3 ubiquitin-protein ligase RNF144B-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 570 bits (1470), Expect = 1.07e-205
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0

Query: 1   MASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICT 60
           MASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICT
Sbjct: 1   MASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICT 60

Query: 61  DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR 120
           DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR
Sbjct: 61  DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR 120

Query: 121 VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW 180
           VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW
Sbjct: 121 VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW 180

Query: 181 HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCG 240
           HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCG
Sbjct: 181 HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCG 240

Query: 241 SEFCYSCGGKWGGAHACRGSAYERTVHRR 269
           SEFCYSCGGKWGGAHACRGSAYERTVHRR
Sbjct: 241 SEFCYSCGGKWGGAHACRGSAYERTVHRR 269

BLAST of MELO3C022695.jh1 vs. NCBI nr
Match: XP_004144476.2 (probable E3 ubiquitin-protein ligase RNF144A [Cucumis sativus] >KGN58470.1 hypothetical protein Csa_001774 [Cucumis sativus])

HSP 1 Score: 506 bits (1303), Expect = 3.08e-180
Identity = 241/268 (89.93%), Postives = 251/268 (93.66%), Query Frame = 0

Query: 2   ASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICTD 61
           +S SSSI   +LSDENYAEELQLQEVITSSLLS PI    +PSPTPHSSTSQ+LCSICTD
Sbjct: 6   SSSSSSILLQNLSDENYAEELQLQEVITSSLLSSPI----NPSPTPHSSTSQLLCSICTD 65

Query: 62  AKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKRV 121
           AKSHSQMFTNRVCTHTFCT CIS HIAAKLEV MAVKCPEPNCGTVLEPEMCGSFVPKRV
Sbjct: 66  AKSHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRV 125

Query: 122 LERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGWH 181
           LERWADALFEAMILK KRLNCPFKDCGAA+IDEG EEGVTAVECGSCWRLFCAECRVGWH
Sbjct: 126 LERWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWH 185

Query: 182 GEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCGS 241
           GEM+CGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHI+CRCGS
Sbjct: 186 GEMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGS 245

Query: 242 EFCYSCGGKWGGAHACRGSAYERTVHRR 269
           +FCYSCG KWGG HAC+ SAYERTV+RR
Sbjct: 246 DFCYSCGAKWGGGHACKASAYERTVYRR 269

BLAST of MELO3C022695.jh1 vs. NCBI nr
Match: XP_038889081.1 (probable E3 ubiquitin-protein ligase RNF144A-A [Benincasa hispida])

HSP 1 Score: 389 bits (1000), Expect = 5.64e-133
Identity = 183/263 (69.58%), Postives = 218/263 (82.89%), Query Frame = 0

Query: 5   SSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIH-PSPTPHSSTSQVLCSICTDAK 64
           SSS F H LSDEN+A ELQLQE ITSSL S P +IP +  SP P  S    +C+ICTDAK
Sbjct: 13  SSSHFLHDLSDENFAYELQLQEAITSSLSSTPAEIPNNNSSPKPQDS----ICTICTDAK 72

Query: 65  SHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKRVLE 124
             S+MFTN +C+HTFCTDCISK+IAAKLE +MAVKCP+P CGT+LEPEMC SF+PK VLE
Sbjct: 73  LASEMFTNGLCSHTFCTDCISKYIAAKLEDSMAVKCPQPKCGTLLEPEMCRSFIPKEVLE 132

Query: 125 RWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGWHGE 184
           RW DALFEAMILK KRLNCPFKDC A MI EGDE+GVTAVEC +CWRLFCA+C+VGWHG+
Sbjct: 133 RWGDALFEAMILKWKRLNCPFKDCSAPMIYEGDEKGVTAVECLNCWRLFCAQCKVGWHGD 192

Query: 185 MKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCGSEF 244
           M+C EF+++RKE     + +D MT+KLA+ KKW++CP+CK+YVEKTVGCVHI+CRCG++F
Sbjct: 193 MECREFEKVRKE-----NTEDMMTMKLADEKKWKKCPNCKVYVEKTVGCVHIICRCGTQF 252

Query: 245 CYSCGGKWGGAHACRGSAYERTV 266
           CYSCG KWGG HAC  SAYE ++
Sbjct: 253 CYSCGAKWGGGHACPNSAYEHSM 266

BLAST of MELO3C022695.jh1 vs. NCBI nr
Match: XP_022956145.1 (probable E3 ubiquitin-protein ligase ARI10 [Cucurbita moschata])

HSP 1 Score: 355 bits (912), Expect = 1.69e-120
Identity = 171/257 (66.54%), Postives = 196/257 (76.26%), Query Frame = 0

Query: 13  LSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICTDAKSHSQMFTNR 72
           +SDE +AEELQLQE I SSL +  I +   PSP P SS S  LC+ICTD KS S+MFT+ 
Sbjct: 32  ISDEIFAEELQLQESIASSLFTSTI-MSNKPSPNPESSGSTTLCTICTDTKSASEMFTST 91

Query: 73  VCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKRVLERWADALFEA 132
            C+HTFC DCISKHIAAKLE +MAVKCPEP C  VLEPEMC SFVPK VLERW DAL +A
Sbjct: 92  GCSHTFCADCISKHIAAKLEDSMAVKCPEPKCAAVLEPEMCDSFVPKEVLERWGDALLKA 151

Query: 133 MILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGWHGEMKCGEFQRL 192
           M+LK K + CPFKDC A M+DE D E   AVEC  CWR+FCA+C VGWHG+M C EFQRL
Sbjct: 152 MLLKWKMVYCPFKDCSAGMVDEEDME---AVECPKCWRMFCAKCGVGWHGDMNCTEFQRL 211

Query: 193 RKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCGSEFCYSCGGKWG 252
            K  GV  D+++ MT+KLA+ +KW+RCPHCKI VEK  GC+HI CRCGSEFCY CG KW 
Sbjct: 212 LKNGGV--DREEEMTMKLADERKWKRCPHCKILVEKIEGCLHITCRCGSEFCYCCGAKWN 271

Query: 253 GAHACRGSAYERTVHRR 269
           G HAC  SAYERT +RR
Sbjct: 272 GHHACPPSAYERTAYRR 282

BLAST of MELO3C022695.jh1 vs. NCBI nr
Match: XP_022980686.1 (probable E3 ubiquitin-protein ligase ARI5 [Cucurbita maxima])

HSP 1 Score: 351 bits (901), Expect = 7.69e-119
Identity = 170/268 (63.43%), Postives = 201/268 (75.00%), Query Frame = 0

Query: 6   SSIFQHH----LSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICTD 65
           S++ +H     +SDE +AEELQLQE I SS  S  +     PSP P SS S+ LC+ICTD
Sbjct: 21  SALHEHQSVFPISDEIFAEELQLQESIASSFTSTIMSN--KPSPNPESSGSKTLCTICTD 80

Query: 66  AKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKRV 125
            KS S+MFT+  C+HTFC DCISKHIAAKL  ++AVKCPEP C  VL+PEMC SFVPK V
Sbjct: 81  TKSASEMFTSTGCSHTFCADCISKHIAAKLVDSLAVKCPEPKCAAVLKPEMCDSFVPKEV 140

Query: 126 LERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGWH 185
           LERW DAL +AM+LK K + CPFKDC A M+DE D E   AVEC  CWR+FCA+C VGWH
Sbjct: 141 LERWGDALLKAMLLKWKMVYCPFKDCSAGMVDEEDRE---AVECPKCWRMFCAKCGVGWH 200

Query: 186 GEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCGS 245
            +M C EFQRL K  GV  D+++ MT+KLA+ +KW+RCPHCKI VEK +GC+HI CRCGS
Sbjct: 201 ADMNCTEFQRLLKNGGV--DREEEMTMKLADERKWKRCPHCKILVEKILGCLHITCRCGS 260

Query: 246 EFCYSCGGKWGGAHACRGSAYERTVHRR 269
           EFCYSCGGKW   HAC  SAYERTV+RR
Sbjct: 261 EFCYSCGGKWNDRHACPPSAYERTVNRR 281

BLAST of MELO3C022695.jh1 vs. ExPASy Swiss-Prot
Match: Q8BKD6 (E3 ubiquitin-protein ligase RNF144B OS=Mus musculus OX=10090 GN=Rnf144b PE=2 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 6.3e-18
Identity = 59/202 (29.21%), Postives = 88/202 (43.56%), Query Frame = 0

Query: 54  VLCSICTDAKSHSQMFTNRVCTHTFCTDCISKHIAAKLE--VTMAVKCPEPNC---GTVL 113
           V C +C   +S  +M   + C   FCT C+ +++   +       + CP+  C   GT+ 
Sbjct: 28  VTCKLCLCEQSLDKMTMLQECQCIFCTPCLKQYMVLSIREGCGSPITCPDMVCLNHGTLQ 87

Query: 114 EPEMCGSFVPKRVLERWADALFEAMI-LKSKRLNCPFKDCGAAM-IDEGDEEGVTAVECG 173
           E E+    VP    + +    FE  + +   R  CP  DC     I  GD      VEC 
Sbjct: 88  ETEI-ACLVPLDEFQLYQRLKFEREVHMDPLRTWCPVADCQTVCHISAGDPGQPVLVECP 147

Query: 174 SCWRLFCAECRVGWHGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYV 233
           SC   FC+ C+  WH E  C + Q    E G     D    +K        +CP C+IY+
Sbjct: 148 SCHLKFCSCCKDAWHEESSCRDSQSAMPEHGALFGTDADAPIK--------QCPVCRIYI 207

Query: 234 EKTVGCVHILCR-CGSEFCYSC 248
           E+  GC  ++C+ C   FC+ C
Sbjct: 208 ERNEGCAQMMCKNCKHTFCWYC 220

BLAST of MELO3C022695.jh1 vs. ExPASy Swiss-Prot
Match: A5PK27 (E3 ubiquitin-protein ligase RNF144B OS=Bos taurus OX=9913 GN=RNF144B PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 6.9e-17
Identity = 63/217 (29.03%), Postives = 99/217 (45.62%), Query Frame = 0

Query: 42  HPSPTPHSSTSQVLCSICTDAKSHSQMFTNRVCTHTFCTDCISKH--IAAKLEVTMAVKC 101
           +P+P   +    V C +C   +S  +M T + C   FCT C+ ++  +A +      + C
Sbjct: 17  NPTPGDLALVPLVTCKLCLCEQSLDKMTTLQECRCIFCTACLKQYMQLAIREGCGSPITC 76

Query: 102 PEPNC---GTVLEPEMCGSFVPKRVLERWADALFEAMI-LKSKRLNCPFKDCGAAM-IDE 161
           P+  C   GT+ E E+    VP    + +    FE  + L   R  CP  DC     +  
Sbjct: 77  PDMVCLNHGTLQEAEI-ACLVPVDQFQLYQRLKFEREVHLDPCRTWCPVADCQTVCPVAT 136

Query: 162 GDEEGVTAVECGSCWRLFCAECRVGWHGEMKCGEFQR--LRKEAG-VSGDKDDAMTVKLA 221
            D      VEC SC   FC+ C+  WH E+ C + Q   L  E G + G + DA      
Sbjct: 137 SDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPGILPTEHGTLFGTETDAPI---- 196

Query: 222 ENKKWRRCPHCKIYVEKTVGCVHILCR-CGSEFCYSC 248
                ++CP C++Y+E+  GC  ++C+ C   FC+ C
Sbjct: 197 -----KQCPVCRVYIERNEGCAQMMCKNCKHTFCWYC 223

BLAST of MELO3C022695.jh1 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 6.9e-17
Identity = 56/222 (25.23%), Postives = 97/222 (43.69%), Query Frame = 0

Query: 51  TSQVLCSICTDAKSHSQMFTNRVCTHTFCTDCISKHIAAKLE---VTMAVKCPEPNCGTV 110
           +  V C IC +    +Q F   +C H +C  C   ++  K+      +   CP P C  +
Sbjct: 134 SGNVSCLICLEDYPPTQTFA-LICNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVI 193

Query: 111 LEPEMCGSFVPKRVLERWADALFEAMILKSKRLN-CPFKDC-GAAMIDEGDEEGVTAVEC 170
           +  +     V   V ER+ + + ++ +  + ++  CP   C  +   D  + +     +C
Sbjct: 194 VHQDAFKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAPGCIYSIRCDRKERKEAVNCKC 253

Query: 171 GSCWRLFCAECRVGWHGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIY 230
           G  +   C +  +G H    C +  +  ++A    D+ + +T  LA  KK   CP C+  
Sbjct: 254 GFQYCFNCNDYEIGDHMPCPCSQVDKWLQKA---SDESENVTWMLANTKK---CPECRSP 313

Query: 231 VEKTVGCVHILCR-----CGSEFCYSCGGKWGGAHACRGSAY 263
           +EK  GC+H+ CR     CG EFC+ C G W    +  G  Y
Sbjct: 314 IEKNGGCMHMTCRKNAGGCGFEFCWLCRGPWTEHGSTTGGYY 348

BLAST of MELO3C022695.jh1 vs. ExPASy Swiss-Prot
Match: Q7Z419 (E3 ubiquitin-protein ligase RNF144B OS=Homo sapiens OX=9606 GN=RNF144B PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.2e-16
Identity = 60/214 (28.04%), Postives = 98/214 (45.79%), Query Frame = 0

Query: 42  HPSPTPHSSTSQVLCSICTDAKSHSQMFTNRVCTHTFCTDCISKH--IAAKLEVTMAVKC 101
           +P+P   +    + C +C   +S  +M T + C   FCT C+ ++  +A +      + C
Sbjct: 16  NPTPGDLAPAPLITCKLCLCEQSLDKMTTLQECQCIFCTACLKQYMQLAIREGCGSPITC 75

Query: 102 PEPNC---GTVLEPEMCGSFVPKRVLERWADALFEAMI-LKSKRLNCPFKDCGAAM-IDE 161
           P+  C   GT+ E E+    VP    + +    FE  + L   R  CP  DC     +  
Sbjct: 76  PDMVCLNHGTLQEAEI-ACLVPVDQFQLYQRLKFEREVHLDPYRTWCPVADCQTVCPVAS 135

Query: 162 GDEEGVTAVECGSCWRLFCAECRVGWHGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENK 221
            D      VEC SC   FC+ C+  WH E+ C + Q +     V   +  A+    AE  
Sbjct: 136 SDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPI-----VLPTEHRALFGTDAE-A 195

Query: 222 KWRRCPHCKIYVEKTVGCVHILCR-CGSEFCYSC 248
             ++CP C++Y+E+  GC  ++C+ C   FC+ C
Sbjct: 196 PIKQCPVCRVYIERNEGCAQMMCKNCKHTFCWYC 222

BLAST of MELO3C022695.jh1 vs. ExPASy Swiss-Prot
Match: Q6NW85 (E3 ubiquitin-protein ligase arih1l OS=Danio rerio OX=7955 GN=arih1l PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 3.4e-16
Identity = 61/228 (26.75%), Postives = 96/228 (42.11%), Query Frame = 0

Query: 32  LLSPPIKIPIHPSPTPHSSTSQVLCSICTDAKSHSQMFTNRVCTHTFCTDCISKHIAAKL 91
           +++P  K    P  T  SS+  + C IC      +  FT   C H FC  C   ++  K+
Sbjct: 139 VINPSKKPKTRPMST-RSSSQDLPCQICY-LNYPNSYFTGLECGHKFCMQCWGDYLTTKI 198

Query: 92  ---EVTMAVKCPEPNCGTVLEPEMCGSFVPKRVLERWADALFEAMILKSKRLN--CPFKD 151
               +   + CP  NC  +++       +    ++     L     ++  RL   CP  D
Sbjct: 199 IEEGMGQTISCPAHNCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPD 258

Query: 152 CGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGWHGEMKCGEFQRLRKEAGVSGDKDDAM 211
           C   +  +  +      +CG   R FC  C   WH  +KC   ++  K+     D D   
Sbjct: 259 CHHVVKVQYPDAKPVRCKCG---RQFCFNCGENWHDPVKCKWLRKWIKKC----DDDSET 318

Query: 212 TVKLAENKKWRRCPHCKIYVEKTVGCVHILCR---CGSEFCYSCGGKW 252
           +  +A N K   CP C + +EK  GC H++CR   C +EFC+ C G W
Sbjct: 319 SNWIAANTK--ECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPW 355

BLAST of MELO3C022695.jh1 vs. ExPASy TrEMBL
Match: A0A5A7UED3 (E3 ubiquitin-protein ligase RNF144B-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00700 PE=3 SV=1)

HSP 1 Score: 570 bits (1470), Expect = 5.16e-206
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0

Query: 1   MASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICT 60
           MASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICT
Sbjct: 1   MASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICT 60

Query: 61  DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR 120
           DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR
Sbjct: 61  DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR 120

Query: 121 VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW 180
           VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW
Sbjct: 121 VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW 180

Query: 181 HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCG 240
           HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCG
Sbjct: 181 HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCG 240

Query: 241 SEFCYSCGGKWGGAHACRGSAYERTVHRR 269
           SEFCYSCGGKWGGAHACRGSAYERTVHRR
Sbjct: 241 SEFCYSCGGKWGGAHACRGSAYERTVHRR 269

BLAST of MELO3C022695.jh1 vs. ExPASy TrEMBL
Match: A0A1S3CDI0 (E3 ubiquitin-protein ligase RNF144B-like OS=Cucumis melo OX=3656 GN=LOC103499194 PE=3 SV=1)

HSP 1 Score: 570 bits (1470), Expect = 5.16e-206
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0

Query: 1   MASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICT 60
           MASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICT
Sbjct: 1   MASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICT 60

Query: 61  DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR 120
           DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR
Sbjct: 61  DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR 120

Query: 121 VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW 180
           VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW
Sbjct: 121 VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW 180

Query: 181 HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCG 240
           HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCG
Sbjct: 181 HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCG 240

Query: 241 SEFCYSCGGKWGGAHACRGSAYERTVHRR 269
           SEFCYSCGGKWGGAHACRGSAYERTVHRR
Sbjct: 241 SEFCYSCGGKWGGAHACRGSAYERTVHRR 269

BLAST of MELO3C022695.jh1 vs. ExPASy TrEMBL
Match: A0A0A0L952 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G646570 PE=3 SV=1)

HSP 1 Score: 506 bits (1303), Expect = 1.49e-180
Identity = 241/268 (89.93%), Postives = 251/268 (93.66%), Query Frame = 0

Query: 2   ASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICTD 61
           +S SSSI   +LSDENYAEELQLQEVITSSLLS PI    +PSPTPHSSTSQ+LCSICTD
Sbjct: 6   SSSSSSILLQNLSDENYAEELQLQEVITSSLLSSPI----NPSPTPHSSTSQLLCSICTD 65

Query: 62  AKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKRV 121
           AKSHSQMFTNRVCTHTFCT CIS HIAAKLEV MAVKCPEPNCGTVLEPEMCGSFVPKRV
Sbjct: 66  AKSHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRV 125

Query: 122 LERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGWH 181
           LERWADALFEAMILK KRLNCPFKDCGAA+IDEG EEGVTAVECGSCWRLFCAECRVGWH
Sbjct: 126 LERWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWH 185

Query: 182 GEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCGS 241
           GEM+CGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHI+CRCGS
Sbjct: 186 GEMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGS 245

Query: 242 EFCYSCGGKWGGAHACRGSAYERTVHRR 269
           +FCYSCG KWGG HAC+ SAYERTV+RR
Sbjct: 246 DFCYSCGAKWGGGHACKASAYERTVYRR 269

BLAST of MELO3C022695.jh1 vs. ExPASy TrEMBL
Match: A0A6J1GX07 (probable E3 ubiquitin-protein ligase ARI10 OS=Cucurbita moschata OX=3662 GN=LOC111457924 PE=3 SV=1)

HSP 1 Score: 355 bits (912), Expect = 8.19e-121
Identity = 171/257 (66.54%), Postives = 196/257 (76.26%), Query Frame = 0

Query: 13  LSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICTDAKSHSQMFTNR 72
           +SDE +AEELQLQE I SSL +  I +   PSP P SS S  LC+ICTD KS S+MFT+ 
Sbjct: 32  ISDEIFAEELQLQESIASSLFTSTI-MSNKPSPNPESSGSTTLCTICTDTKSASEMFTST 91

Query: 73  VCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKRVLERWADALFEA 132
            C+HTFC DCISKHIAAKLE +MAVKCPEP C  VLEPEMC SFVPK VLERW DAL +A
Sbjct: 92  GCSHTFCADCISKHIAAKLEDSMAVKCPEPKCAAVLEPEMCDSFVPKEVLERWGDALLKA 151

Query: 133 MILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGWHGEMKCGEFQRL 192
           M+LK K + CPFKDC A M+DE D E   AVEC  CWR+FCA+C VGWHG+M C EFQRL
Sbjct: 152 MLLKWKMVYCPFKDCSAGMVDEEDME---AVECPKCWRMFCAKCGVGWHGDMNCTEFQRL 211

Query: 193 RKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCGSEFCYSCGGKWG 252
            K  GV  D+++ MT+KLA+ +KW+RCPHCKI VEK  GC+HI CRCGSEFCY CG KW 
Sbjct: 212 LKNGGV--DREEEMTMKLADERKWKRCPHCKILVEKIEGCLHITCRCGSEFCYCCGAKWN 271

Query: 253 GAHACRGSAYERTVHRR 269
           G HAC  SAYERT +RR
Sbjct: 272 GHHACPPSAYERTAYRR 282

BLAST of MELO3C022695.jh1 vs. ExPASy TrEMBL
Match: A0A6J1IU95 (probable E3 ubiquitin-protein ligase ARI5 OS=Cucurbita maxima OX=3661 GN=LOC111479983 PE=3 SV=1)

HSP 1 Score: 351 bits (901), Expect = 3.72e-119
Identity = 170/268 (63.43%), Postives = 201/268 (75.00%), Query Frame = 0

Query: 6   SSIFQHH----LSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICTD 65
           S++ +H     +SDE +AEELQLQE I SS  S  +     PSP P SS S+ LC+ICTD
Sbjct: 21  SALHEHQSVFPISDEIFAEELQLQESIASSFTSTIMSN--KPSPNPESSGSKTLCTICTD 80

Query: 66  AKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKRV 125
            KS S+MFT+  C+HTFC DCISKHIAAKL  ++AVKCPEP C  VL+PEMC SFVPK V
Sbjct: 81  TKSASEMFTSTGCSHTFCADCISKHIAAKLVDSLAVKCPEPKCAAVLKPEMCDSFVPKEV 140

Query: 126 LERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGWH 185
           LERW DAL +AM+LK K + CPFKDC A M+DE D E   AVEC  CWR+FCA+C VGWH
Sbjct: 141 LERWGDALLKAMLLKWKMVYCPFKDCSAGMVDEEDRE---AVECPKCWRMFCAKCGVGWH 200

Query: 186 GEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCGS 245
            +M C EFQRL K  GV  D+++ MT+KLA+ +KW+RCPHCKI VEK +GC+HI CRCGS
Sbjct: 201 ADMNCTEFQRLLKNGGV--DREEEMTMKLADERKWKRCPHCKILVEKILGCLHITCRCGS 260

Query: 246 EFCYSCGGKWGGAHACRGSAYERTVHRR 269
           EFCYSCGGKW   HAC  SAYERTV+RR
Sbjct: 261 EFCYSCGGKWNDRHACPPSAYERTVNRR 281

BLAST of MELO3C022695.jh1 vs. TAIR 10
Match: AT3G14250.1 (RING/U-box superfamily protein )

HSP 1 Score: 231.1 bits (588), Expect = 1.0e-60
Identity = 110/266 (41.35%), Postives = 152/266 (57.14%), Query Frame = 0

Query: 13  LSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSST-----------------SQVL 72
           ++DE+YA E+ LQE + SS  +  + +  HP    +  T                 S+ L
Sbjct: 35  IADESYATEIHLQEAMFSSFGASTVGVNHHPQVHRNLVTLVKQEPEIKAENEPMEPSRRL 94

Query: 73  CSICTDAKSHSQMFTNRV-CTHTFCTDCISKHIAAKL-EVTMAVKCPEPNCGTVLEPEMC 132
           C IC D K  S +F     CTH +CTDC  +++A K+ E    +KCP+  C  ++EP  C
Sbjct: 95  CMICMDEKPSSDIFRGTTNCTHAYCTDCTVRYVATKIKENASRIKCPDVECTRLIEPYTC 154

Query: 133 GSFVPKRVLERWADALFEAMILKSKRLNCPFKDCGAAMI-DEGDEEGVTAVECGSCWRLF 192
              +PK V +RW   L E++I    +  CPFKDC A M+ +E  +  VT  EC SC RLF
Sbjct: 155 RDLIPKDVFDRWEKILCESLISSWDKFYCPFKDCSAMMVNNENGDANVTQTECRSCHRLF 214

Query: 193 CAECRVGWHGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGC 252
           C +C+V WH  + C EFQR       S D+DDA+ +++A+NK+WRRCP CK YV+K  GC
Sbjct: 215 CVQCKVTWHAGIGCDEFQRFGNTKKKSSDEDDALLIQMAKNKQWRRCPSCKFYVDKVEGC 274

Query: 253 VHILCRCGSEFCYSCGGKWGGAHACR 259
            HI CRCG +FCY CG  W  +HAC+
Sbjct: 275 QHIKCRCGYQFCYGCGSVWSSSHACQ 300

BLAST of MELO3C022695.jh1 vs. TAIR 10
Match: AT3G53690.1 (RING/U-box superfamily protein )

HSP 1 Score: 204.5 bits (519), Expect = 1.1e-52
Identity = 93/212 (43.87%), Postives = 130/212 (61.32%), Query Frame = 0

Query: 40  PIH--PSPTPHSSTSQVLCSICTDAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVT-MA 99
           PIH     +  S T+   C IC D+KS  + F    C+H +C DC+SK+IAAKL+   ++
Sbjct: 97  PIHREKGQSSSSKTATFDCEICVDSKSIIESFRIGGCSHFYCNDCVSKYIAAKLQDNILS 156

Query: 100 VKCPEPNCGTVLEPEMCGSFVPKRVLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGD 159
           ++CP   C   LEP+ C   +PK V +RW DAL EA++++SK+  CP+KDC A +  E  
Sbjct: 157 IECPVSGCSGRLEPDQCRQILPKEVFDRWGDALCEAVVMRSKKFYCPYKDCSALVFLEES 216

Query: 160 EEGVTAVECGSCWRLFCAECRVGWHGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKW 219
           E  +   EC  C R+ C EC   WH EM C EFQ+L   A     +DD +   +A+ KKW
Sbjct: 217 EVKMKDSECPHCHRMVCVECGTQWHPEMTCEEFQKL---AANERGRDDILLATMAKQKKW 276

Query: 220 RRCPHCKIYVEKTVGCVHILCRCGSEFCYSCG 249
           +RCP CK Y+EK+ GC+++ CRCG  FCY+CG
Sbjct: 277 KRCPSCKFYIEKSQGCLYMKCRCGLAFCYNCG 305

BLAST of MELO3C022695.jh1 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 156.4 bits (394), Expect = 3.3e-38
Identity = 85/258 (32.95%), Postives = 126/258 (48.84%), Query Frame = 0

Query: 7   SIFQHHL---SDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICTDAK 66
           S +QH L   +D  +A +L  + ++  S+++P      H  P    +  +  C+IC +  
Sbjct: 257 SSYQHVLVARNDVKFAYKLARESIL--SMVTP------HEDPRQAKAVLKEECAICFNDI 316

Query: 67  SHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKRVLE 126
               MF+   C H FC  C+ +H+  KL   MA KCP   C + L  + CG  +  ++ +
Sbjct: 317 VAEGMFSVDKCRHRFCFQCVKQHVEVKLLHGMAPKCPHDGCKSELVIDACGKLLTPKLSK 376

Query: 127 RWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAV----------ECGSCWRLFC 186
            W   L E  I  ++R+ CP+  C A M      E   ++           C  C  LFC
Sbjct: 377 LWQQRLQENAIPVTERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVRRCVECRGLFC 436

Query: 187 AECRVGWHGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCV 246
            +C+V WHG + C E+++L  E       DD     LA NK WR+C  C+  +E + GC 
Sbjct: 437 VDCKVPWHGNLSCTEYKKLHPEP----PADDVKLKSLANNKMWRQCGKCQHMIELSQGCN 496

Query: 247 HILCRCGSEFCYSCGGKW 252
           HI CRCG EFCY+CGG W
Sbjct: 497 HITCRCGHEFCYNCGGGW 502

BLAST of MELO3C022695.jh1 vs. TAIR 10
Match: AT5G37560.1 (RING/U-box superfamily protein )

HSP 1 Score: 152.1 bits (383), Expect = 6.2e-37
Identity = 71/205 (34.63%), Postives = 104/205 (50.73%), Query Frame = 0

Query: 56  CSICTDAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGS 115
           CSIC+D      M  N  C H  C  C+ + +  KL   +   C E  C + L  E C  
Sbjct: 181 CSICSDKTDAEHMLLNDKCLHRHCFSCVKQQVKVKLRSGIVPPCLEDGCKSELTLESCSM 240

Query: 116 FVPKRVLERWADALFEAMILKSKRLNCPFKDCGAAMID-----EGDEEGVTAVECGSCWR 175
            +  +++E W   + E +I  ++++ CP++ C   M       E ++  V A  C  C  
Sbjct: 241 VLTPKLIEMWKRKMEEDLIPDAEKIYCPYRSCSMLMSKTELSREAEQSNVRA--CIKCSE 300

Query: 176 LFCAECRVGWHGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTV 235
           LFC +C+V WH ++ C +++R+  E  V    +D M   LA ++ WR+C  CK  +E T 
Sbjct: 301 LFCIDCKVPWHSDLSCADYKRIHSERLV----NDMMLKVLANDQMWRQCSECKHMIELTE 360

Query: 236 GCVHILCRCGSEFCYSCGGKWGGAH 256
           GC HI CRCG EFCY CG KW   H
Sbjct: 361 GCNHITCRCGYEFCYRCGHKWTKYH 379

BLAST of MELO3C022695.jh1 vs. TAIR 10
Match: AT3G45540.1 (RING/U-box protein with C6HC-type zinc finger )

HSP 1 Score: 142.9 bits (359), Expect = 3.8e-34
Identity = 74/242 (30.58%), Postives = 122/242 (50.41%), Query Frame = 0

Query: 17  NYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICTDAK-SHSQMFTNRVCT 76
           +YA +L  + +++       I I ++P P    +T +  C IC D   + +QMF    C 
Sbjct: 68  SYAYKLARETIVSE------ISISVNP-PRQPKATRKTTCKICLDDDINENQMFCVGKCR 127

Query: 77  HTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKRVLERWADALFEAMIL 136
           H FC+DC+ +HI  +L     ++CP   C T L+   C + +  ++ E W   + E +I 
Sbjct: 128 HRFCSDCMRRHIEVRLLEGSVMRCPHYRCKTTLKFGGCINLLTPKIREMWQQRIKEDLIP 187

Query: 137 KSKRLNCPFKDCGAAM----IDEGDEEGVTAVECGSCWRLFCAECRVGWHGEMKCGEFQR 196
            + R+ CP   C A M    +    +E      C  C ++FC +C+V WH  + C ++++
Sbjct: 188 VTGRIYCPNSRCSALMSETELSISTKEDEVRRCCFKCGQIFCIKCKVSWHSNLSCNDYKK 247

Query: 197 LRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCGSEFCYSCGGKW 254
           L         ++D     LA  K+WR+C  C+  +E + GCV + CRCG +FCY CG + 
Sbjct: 248 LNPYP----TENDGKIKALANQKRWRQCGKCQHMIELSKGCVQVKCRCGHKFCYRCGVQA 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008460341.11.07e-205100.00PREDICTED: E3 ubiquitin-protein ligase RNF144B-like [Cucumis melo] >KAA0053550.1... [more]
XP_004144476.23.08e-18089.93probable E3 ubiquitin-protein ligase RNF144A [Cucumis sativus] >KGN58470.1 hypot... [more]
XP_038889081.15.64e-13369.58probable E3 ubiquitin-protein ligase RNF144A-A [Benincasa hispida][more]
XP_022956145.11.69e-12066.54probable E3 ubiquitin-protein ligase ARI10 [Cucurbita moschata][more]
XP_022980686.17.69e-11963.43probable E3 ubiquitin-protein ligase ARI5 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8BKD66.3e-1829.21E3 ubiquitin-protein ligase RNF144B OS=Mus musculus OX=10090 GN=Rnf144b PE=2 SV=... [more]
A5PK276.9e-1729.03E3 ubiquitin-protein ligase RNF144B OS=Bos taurus OX=9913 GN=RNF144B PE=2 SV=1[more]
Q6T4866.9e-1725.23Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 G... [more]
Q7Z4191.2e-1628.04E3 ubiquitin-protein ligase RNF144B OS=Homo sapiens OX=9606 GN=RNF144B PE=1 SV=1[more]
Q6NW853.4e-1626.75E3 ubiquitin-protein ligase arih1l OS=Danio rerio OX=7955 GN=arih1l PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UED35.16e-206100.00E3 ubiquitin-protein ligase RNF144B-like protein OS=Cucumis melo var. makuwa OX=... [more]
A0A1S3CDI05.16e-206100.00E3 ubiquitin-protein ligase RNF144B-like OS=Cucumis melo OX=3656 GN=LOC103499194... [more]
A0A0A0L9521.49e-18089.93Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G646570 PE=3 SV=1[more]
A0A6J1GX078.19e-12166.54probable E3 ubiquitin-protein ligase ARI10 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1IU953.72e-11963.43probable E3 ubiquitin-protein ligase ARI5 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT3G14250.11.0e-6041.35RING/U-box superfamily protein [more]
AT3G53690.11.1e-5243.87RING/U-box superfamily protein [more]
AT5G60250.13.3e-3832.95zinc finger (C3HC4-type RING finger) family protein [more]
AT5G37560.16.2e-3734.63RING/U-box superfamily protein [more]
AT3G45540.13.8e-3430.58RING/U-box protein with C6HC-type zinc finger [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002867IBR domainSMARTSM00647ibrneu5coord: 123..186
e-value: 8.1E-15
score: 65.2
coord: 201..257
e-value: 0.018
score: 21.2
IPR002867IBR domainPFAMPF01485IBRcoord: 124..186
e-value: 1.2E-10
score: 41.4
coord: 209..255
e-value: 3.4E-8
score: 33.6
NoneNo IPR availableGENE3D2.20.25.20coord: 140..187
e-value: 9.4E-6
score: 27.6
NoneNo IPR availableGENE3D1.20.120.1750coord: 188..268
e-value: 1.3E-15
score: 59.9
NoneNo IPR availablePANTHERPTHR11685:SF327RBR-TYPE E3 UBIQUITIN TRANSFERASEcoord: 13..248
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 51..132
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 205..257
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 127..191
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 49..134
e-value: 4.2E-19
score: 70.2
IPR031127E3 ubiquitin ligase RBR familyPANTHERPTHR11685RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAININGcoord: 13..248
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 74..83
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 56..100
score: 9.951762
IPR044066TRIAD supradomainPROSITEPS51873TRIADcoord: 52..266
score: 28.979259

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C022695.jh1.t1MELO3C022695.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity