MELO3C022605.jh1 (gene) Melon (Harukei-3) v1.41

Overview


Name	MELO3C022605.jh1
Type	gene
Organism	Cucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Description	Thiamine-phosphate pyrophosphorylase
Location	chr10: 16203680 .. 16227124 (-)
RNA-Seq Expression	MELO3C022605.jh1
Synteny	MELO3C022605.jh1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSstart_codonstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

TCAGCGCCCCCGTCACACACTGCATTCTCAGCTTCTCCATTCTCAGCCGTCCGACGAAATTTCCGCCTCCTCCTCCCTCTCACACTTTGTCGCCTCCTTGAGCCATTAGAGTTACATCTCTGTTTTGTTTCTCAGAAACGCAGTTGTTCAACCCCTAGCCGGCGCCGTCGGTGAGATGGTGCCGCTGCCCCTCATTTCTCAGATCCCTAAGGTCGTATTTTTTATATTGTTTCCTTGGATAAGAAAATTTAGAGCAGCCCTGTAGTTTTGTTGGCTATAAAAAAACTTGTTGGAATTACAAACTATTAAATATTCAAATTAAATCTATCCATTCGATAAACCCACCAAAATACAATGCACAGGTTAAATAGAAAACTTAACTTTGACTTTAGTTTGTTTGAATAGAACTGAGTCTTGTTGAATATAGAAGAAACTTCATGGAAATTGGAGGTGGCTAGGCAGATATTATTATATTTGTGGAACAGGAAACATAAGTGTTAAAGTTTATGAAACCATCGTGGTTGTCCTATGGGTAAAAAAAAGAAACATAATCCTTAGCTGTGGGTTCAATCCATAATGACAATGACCACCTAGTTGAAAATTAATTTTTTACACTCAAAAGTTGTTGTCCCCCTTGGCTTGGACACTAACGAATATCTAAAAAAAAATATTTAAAATCCTCTCTTATCAGACCTATTTAGACCAATTTATTTTTATTTATTTAAAGAAAAACAAGATTTCAAGATTCCTGCCCCATTTTTATTGCATCCTAAATTGAGTTGAAATCTTTTCCAAATTAAGGGATTCCAAGCAGGGGGTTAGCTTTATTTATTATGAAAATATATTTTATAATTTATAGGTTTTACTCTCTGTGCCTTAATCTTTACTGTTGTCTATTTTAGTTCAATCAAGTTTCTAGATTTTGTATGGCCATGAAGAAGCAGGAAGAAATGGTTGTAGCTTCAAGTGATCGATATGAGACGCGGATTCCGCATGTGTTGAGTGTTGCTGGTTCTGATTCGGGAGCAGGGGCTGGAATCCAAGCAGATCTTAAGACATGTGCTGCTCGTGGAGTGTATTGCTCCACTGTGATAACTGCTATTACTGCACAGAATACTGTGGGGGTTCAGGTCGTTATCACACACTCACTATTATTATTATCTATATTTATGTATTGTTTTTTTTGTTTTGGCTTGTAAGAATAATGGGAAGGACTAGTGTATGAATAATCAAATGTTCACTGGCTACTTGTCTGGCTGTTTTGGTATATTGGAGTGTAGAAAATGGTTTAACATGCTGCAATGGTCAAATTCTAATGTTTTTGAGAGTTTGGGATGATTTTCGAAATCAGGGTGCTTTTGAGTGAAAGCTAATACTTCGTTAGCACTTTATTAAATGTTGTCTAGGGTGTGTTTAGGGTTGCTTTTGGCATGGTGAAAAGCACCTTTCTCATGGCCAAACTACTAGTGATATGATCAAAGAGTTCTTCCTCCATCCATTTGGTGAGAAAAGCTAATTCTTGTGCCTTGCCGAAGTGTGCACTGTTTTGTGAGGTCTTTGGTATGTGTGGAATAATAGTGTTTAGAGGGTTGGAAAGGGATCCTAATGATGTTTTGTCTTTTTGTTGGATTTTATGTTTCCATTGGGTGCGTTCGGTTTTGAAGATTTTTTGTAATTATTAGTATTTTCAAGGTGACCCAAGTTGCATAATTGGAATGTTTTTACTACAGTATTTAAGGTTATATTATTATTTAGTTAAAGAAGTTGTTAGGATACTTGGTTATAAATTGAGATAGAGGGAGGGAGTAGTAAGACAATACTGTGGTGGTTGAATTAGGGTTTGAGAGAGATCTCAAGAGGGGAGAGTCCAAGTACCTTGAATAACTTGACTATCTTGTAAGTTCTAGACACATACAAAGGTACTAGATTCGATTTGGTATAAGAACAGTTTGATTATGACCATGGGGTTGAGAGTTATATATAAAGTTAAGAGAAACAATATTAAACGCTTTATGTGAAGCAATGGACCTTTGAAGAATCCCTTGGATGAGCAAATGAAATTGATGTTTCAAACGATATAGAGTACAATGGCACTGAGTTTTGGTCTATTTAGAGAAGTACTTAACACATATTCTACAAACACTTAAATACATCCATAGTTCTCTTAATAATTTTCAAAACCGCTTTTAAATACCTAAGTGAACATTATGAAGACTTTGAGTAGTACTTTTGACTAGCATAAAAGACTTCTCACCTTTGCAAAAATCATCCTAAACTCACTCTTAGAATATATTCGTTGTAGTTACTAGTTAGCTTTTGCTTGTTTTGAAATAAAACTAAACTTAAAATTTTGTTTAACACTAAGCTGCAATAACTTTTTCAGGATGTAAACATTGTTCCGGAGGGCTTTGTTTCAAAGCAGCTGAAATCTGTTCTCTCTGATATGCAAGTGGATGTGGTGAGTATAATTAAATTAGGTTTTAGAGTAAGCCCTTCGTAAATCCATCAATATAGATTAAATAAGAAATTTCAAGGTATGGTTGCCTACCGAGTTTTCTTAGGAGCTCATGCTAACAAGAATGTGGACTGTTCATGTATGGGATATTGCACTGGGTAGCTGTTATTGTTCTTTCAGAGTACATACTAATATGTACTTTATTTAAGCTGGTAAGCATGTATAAAAGATTACTAATGATTACATTAATTTAGGTGAAAACAGGAATGCTACCTTCTACTGGCATTGTTCAAGTTCTACATCAGTGCTTGAAGGAGTTTCCTGTTCGAGGTATAAAGTAATTAAGGAAGTTGCTGGTGGATTTCTTTGATTTAATCGTGGTTCAAATTTTAATGTAATTAAAGGCAGCTTTGGTGGTTGATCCTGTCATGGTGTCTACTAGTGGAGATGTTCTCGCTGGTCCTACTATTATTTCAGTGTTACAGTAAGTTGATGCTTTCTTGAAATGGAATATCTTCAGAACTTCAAGGGAATTCTATTTGTATGATTATGCATTTTTTAAATATCTTCATAATTGTGACACTGTTTTCTTGTTTAAAAGGCTTCTGAAACTTATGTGTGAAGCTCAAACTTTTAACGTTAAAGCTTACTTCTGACGCACGTTTATAAGAACTTATTGAGCCTTGAAATCCTCATGAATCACACTATAACTTGAAAAATAATACTCTTTTGTTCCTCTCTCCACCATGATTTTAGATGGCGTCCATTCATCATAGTAAGAGGTATATGAATCTGCCTACAATGTGATGTTTTATCTTTCATGTGGTATTGCTACTGTTTTCACTTTCACTTGTTAGATTGAGGGTTTCAAAACCCCTCTGCTCGTGACATAGGCAGTTTTTGAAACTTTTCTTCTATACAATTTCTGGCATGTTTCTAATAAATAAAAGAACTCATATTATCACTGAAATATGAGGGACTACACAGGGTTGATGGACATTTGGAGAGCACTCATATTATCGCTGAAAAGTTCAGGAGCTTTAAGTAATGAGATTTCAATGTCAGATAGCAGGAATGTGATCCAAGTGAGTTCTGCGGTTGCGTTCGCCATAGCAAGATATTCTACCTCAACGCTGGATCTAGAGACAGTTGGTTGCTTCTTGGAAACCCATGAGATGAGGTTGGATCCAAGATACACACAAATGCCCGAGATGCTCCTCCTTGTGTTTGGACAACCAACTAATCTGCATCACTAAAAGCATAGAGCTTAAGGCAATTTATTTTTAAGAAAAGTGATGCCATGATGCAAAGCATGCTTAGAATGCGTTTAACTTCCCTGAAGTGCTGCAATGTGGGTGGTTGCATTTTTTACAAGCTTTGTTGATGGCAAAGGCGATGTCAGGTCTTGTTAAGGTGAGGTATTGGATGGACCCAACAAGGCCTCTATAAGTGGTAGGATTGACTAGTTCATCATCTCTTGGGCATTTGTTCTTGCTTGTTGCCGTTGGAGTAGATATTGGAGAAACATTTAGTTAGCCTTATAGAATATCTACCGTGTATACTTAGTTTGAGTGATGTGAAAACCATGGGGTTTATGCTTTAGTTCAACTTCTAAGAAGTAGTGAAGGATCCCAAAATCTTTTAAAGCAAATTCTTGACCTGACTTGGTTACTAAGGTGGCTATAAAGGTTGTGTCATTGCGCGGTGAGGATAATATTATTAATGTAAAAAAGCATAAGAACTAAGGTACTAGAATTTTTGGAAAGGAAGAGAGATGGGTCGAAGGTGCTACATGTAAAACCTATGTGAGTAAGGAAAGTGGACAATCTATCAAACCAAGCTCTAGGTGCTTGTTTTAAGCCATAAAGTGATCGTTTCAATAAATAGACACAATTAAGATGAGCAAGGCAAGTGAAGCCTGGAGGTTGAGCCATGTACACTCTTTGAGATAACCGTGGAGGAAGGCGTTTTTGACATACATTTGTTTCAGGGCCAGTTGAAAGAAGCAGCAAGAGCAAGTACTAATCAAATTGTTGTTGGTTTAACAGCAAGGCTAAAGGTTTCCTCATAGCCAAGACCTTCAATTCAAGAATATCCTTGAGCTACTAGTCTCGCTTTATGTTTCTCTATGGTTCCATCTTCTTTGAATTTTCTTTTAAAGATCCACTTGGAGCCAACTATGTTGGTGTTGGGTGGCTTAGGTACAAGTTTCCATGTTTGGTTCTCATGTAAGGCTTGAGTTTTTGCCTCCATTGCATCTTTCCATACTGAAATTTTGAGTGCAGAGCGAGATGTCTTTGGTTCAAGAGATGGGCTTCAAGTGGCACTAACTAGGAAGGTTACGTAGGAATGGTTTTTGTTTCACCTTGGACCAAGTGACCATGGAATTGAAAGAGAGAGCTGTTACTGTTGGTTTGTGTAGGACGGGCTACATGATCAGGAGTTGGTAATGGAGGCAATGAACCTGTAGCATCGTCAAAAGGTAAGTCATAAACAAAGAATTAGAAATGTAGGCAAATCACTAAAATTAGATTTAGTTGAGGGTTGAGAGATCTCATGGGGAGAGGATGCAGTTGACTGTTTTGGTGTTGAGGTAGGCGGTTGATCTAATATCTACCCATATGATTCTTGCTCAAGGTTTTGACATTCGTTTAGGCTATGTTCTTCCACACCAATTCGTTCAGAATTTTGATCGTCTAGGGACATGAAATTGCTTGATGGTTCTTGGCTGTTGGTATCTGATGTCATGGTAACAAGTGCTTTACAATCGTTGGGATCTCCAAAGAACATTTTGGTCTTACATGGTCTGACAGTCAAAATTATTTGAGTTGAAAGTAGGATGGAGAGGAGTTTAAGCGATTGTTTCTTGGCCAATCTTGGAGTTGATTGTCTCATCTGATTTGTCACTTTCTGCATCTAAAAATGTTGTAACATCCAAAATAGTTAATTATGTGGTGTGTTGATTCTGTTATGTAAGGCATGGTTTCTTCATCAAATATAACATGTCGAGACATACACACACACAATTGTAGTAGGGTCTAAAGGGAAATGCATTTCATCAAGTGTTTCACGACATAACTTGTATGCATGACACATGGCAGGGATTTTGAGAGGCACTTAATAATAAAAATTATTAAGTGTGCTCGACCAAATAAGCTAACAAGCATATTTATAGTCATGAATGGTGGGTATTCATGATCATAGACATCATGGGTCCATTCCCTAATTTGTAGGAAGGTGGTCCTAGAACTATGCAAGGTGGGAAGTGGATGAAATAGGGGGAAAGTGGTCATGTTCTCCACTATCCTAAAAGGTAGGAGTAAATGTGGGAAGATAGAAGAGTGGCCCTAATTATATGAGTAGTGGGTGATTTTAACCCACTAGGGACCCACCTGACCCTTCTAAAATTACTTCTCCCACACCTGGATGAGCTTGGGGATTGTTCAAGGCATCTCAAAATAGATAGGTTATCTTAGAGATCTCAATATCTCAAGTATTCGTTAGTTCACACCTTGAAAAGGAAGATCTCTCACGGTGGGAGATTTCTAGTCTTGAGTCTTGTAAAGTGGTTTTGATAGAGCTTTGATGAATAACGTGTGCTATATTTTCACCATGTATATGTGCATCCCTCCTACCTCTCTCCCTAGCCGGGTTATGTGTTGATCGTTTGAATTGAAAATGTCCTTTGATTGTTAGGATATCGCCCTAAAGTTAACACAAATAATCCTAACAAAGGCAACAAACTGTAATCTTTATAATAATCAACCAAACCAGAAATTCACAAGATGTTTAAGCAAGAAAATAACAAGAAAATACAAGAGAATATTGCAAGAAGAAAGAGAGAACAAACACCGGTTTTATGTGGAAACCCTAGAACAGGTAGAAAAACCATGATGCTATTTTCTTATTATTTTTCTACATATTATAATGATACAGGGAGCATTTATTTATAGGCTGTAAAAGCCAAATAAAATAATAGAAGATAATTAGGGCACTATAAAATCTCAACAAACGCTCGGGCTTTCAACATACACCAAGCCCGCTAATTCTAACGGAAAACACGAAACAAACCAAACTCCTTAGAGAAGGCTGGCATCCTCTCCCATAAAGCTCCAGCGGCCTTTTACTACAAAAAAAGCCCCTTCTTTTATATCTGTGAATAACTGAGGGTCTAACAACCTTCTAGTGTTCTGAGTGTCATTGTGGGTGTTTTGGGCAAAGTTCATGAAGACTTGTCAAGTAGTGTCTTGACGTCACACGGGGTTAAATGGTGAAAAAACTGTACCTGGGATTCTCTCTTGTGACACCCAAATTCCCCAATAGGGCAATAGTTTGCGAATTGAATCAAAATAGTTCCATTTTTTAAAATTCAGAAGTCTGTTTGATCTTTATATGTTAGTTTTGCATAAATCTTTGACATGTCTTTTGCTTGCAGGGAAGAGCTTCTACCAATGGCGGACTTGGTAACTCCAAATTTGAAGGAAGCATCTGCCTTACTTGGTGGTATGCCACTTAAAACAATTTCTGACATGCGTCATGCTGCAACGTTAATCCATCAGATGGGATCAAAGTAAGTTCTTAATCTCTTAGTCATCAGATAAGCTTGCCTTCTTTATAATTTCATTGGTATCAATGAAAGGCACCCATGTTTCTCGTAATAAGAAAAGGAGAATAAAAGGAAATTTTCTGTTGCCTTGCCTCTGGTTACATAGACCGACTTTGACACTCATTTTTTCTAATCGCTGCTCGTCTTGTAGCTGTATTTTTATTTCTATTTGAAGATTTTTAAGCTAAATTATCAAAAATACCTTTGAATTTTGCAGGTGCCTCTATTCTTTCAAAGTTGCACCTTTCATTAACGTTTTAAAACTACTCTAGGAGTACAAATCCTTTAGAATTCAATTTTCTATTCTAGATCAGCACCACACGGTTTCATATTCTGGATCAGCACCACGGCGAAAACATTTTCAATTTAGAAATTGTGCAACCTAATTTGAAAGAGAAAATGATGTTAGACATTTGAAGAATATGAGCTAAGCAAAGATGTGGAAGAAATAAACTTAAAGAAGCAAGGATACCGCGAAGAAGAAGAAATTGCTTGTGGGAATAAAAACGACTAAAACATTTTCAATTTAAAGATTGTGCAATCTAATTTGAAAGAGAATGGCCGTTGACAATTGAGGAAAAATCGGGGAATAACCCACCTAAAATTTCAAACACGGAAGAAAATATGATCAAAACTCTAAGCGTTGAAGTGAAATTTTTTTACAACAAAAAGCAAGGAGGAACTGAATTACCTACTTTGGACAAAGAAGATGAAGTTGGCAATGTTGTAGAGTAAACCAAACCAATCAAAGTTGATGTTTTTGCACACAAATGAAGAAACAATTCTCTACGAAGATGAGGATGAATTTTAGGCTACACTAATCCTGGATGGATTGCAATTTATGCAAGAATCAATAAAGAAAAAAGAAGTAGCAATACGTAGATGGATATTATTGATGTTGGATGTTCCAAGATGCCTCCATTCGACCGACTTACTTTTTTATTTTTACCTTCATTTTCGTTTCAAAACTAGAGGACGAGTTTTTTTTTTGGGAGGGAGAGAAATGTTGTAGTAGGAATATGATATTATGGTCAAATCCTTAATTTATTAGGATTACGGTTAGTTTCTTTGTTTTATTAGGATTAAAATTAGTTTATTTTATTATTTAGAATTAGAATTAAGTTTCTTTTATTAATTAGGATTAGGATTAAAATTAGTTTACTTTTGTTAAGTTTCTCTTGGTAATTAGGATTAGGATTAGTTTACTTTTGCTATAAATAGGGTAGTTGTCTTCTTGTATTCACAACTTGAAAAACATTAATACAATTCTCTCATTTAAGTTACATCAAAGGGTCCGTAGATGATGAAGATACATTTAATTTGTCGAAGCCTTTAGATCTTTAAGAGACTGGTCTTCAGTTTGTAGATGATGAAGATACAATCAATTTGGAAAAGATCAATTCGTTGAAGAAAAGACAATTTATTCGTTAAGAAGAGATGCTCAATTTATTGAAGTCACAATCTATTTGTTGAAGAAGAGTGCTCAATTTGTTGAAGATGAGATGATCTATTCATTGGAGAAGAGATGCTTAATTTGTTGAAGAAGATCACTTTGTTGAATTCTTTGGATACTGATTTTTGGATCTTTGCCAGAATGAATCAAATGATGGTTATCCTCAATTTATAGAGTTTTGGGAGGCCATGTGAGCTTGTGTTAAGGTACCAAATAGTACCTTATGTTTCTATTAAGATTTTCAACGTAATCAAGAGAAAACTTACGGCCCGAACTTAGAAACATGGATCATTCACACTCAAACAAAACAAAATACTAACAAGAACAGAACCAATGCTGGAAAATAAAGAGACAACGCTGGAAACACAAGAATGAAATAATAAGAACAAAGGAACCTGGAACTTCTGCCTTCCTACCCCGCAAGGAGGAATTCAGCCTATCTTCTTCTCCACCAAAATTTTCGTACCCCCCTTTGCTCTCCATCCCACCTTTTAATTCCCCTTACTAACTTACTACTGGTCCCCACTTGGATGGTCCTCAACTTCCTTGCTTTCCTCCCCACTCCCACGTGTAGCTTCCCCATTTGTTACATTGTTTTTCTTCTTCCTTCTATTATAAGTGAATAAAATTGGGGCCTAGCATCACTCTCCTCCTCTAAATCCACCTTGAACAAGGTGGAACTCAGGGAATTGGTGCTTAAAGTGCACAATCTTCCCAAGTGGCTTCATGAGGGGGTAGACATTTCCAACTGACCAGTATTTCCCACTCCTTCGTAGTCTGATTCTTGCGGTAACTGTACACTTCCTCTGGCTGTGTGATCCACTCGTGATTTTCATTAATATAGGGGTCAAGCTGTTGTACTTATTCAAAGTTTCCTACTGCCTTTTTAAGCTGAGAGGCGTGGAACATGGGGTGAATGACTAATGAACTCAGTTGTTCCAACTTATAAGCTACTGCCTCAATCTTCTCTAGAATCTTATAGGGCCCAAAGTACTTCGGCAACAGCTTCTCATTACGCTTCCTTCTTAAGGATACTTGACTGTAGGGCCTGACTTTTAGGAACACATGATCCCCTACCTGAAATTCTACATCCCTTCCCTTCACGTCTGCAAACTTTTTCATCCTTTCCTAAGCCACTCGTAGATGTTCCTTTAATGCTCCCAAGATCACATCTTTGTCCCTCAGTTGTTGATCAAGAGTGGAGTTTGGTGTGTCCATGTCTCCGTATTAGATCAAGGGCGGGGGTAATCTTCCATAGACAGCCTGGAATAGGGTGATTCCAATTGAGTTATGATACATGGTATTGTACCAGTCTTCTGCCCAGTGTAGCCATTGACTCCACTCCTTCGGTTTCTCACCACAGAAGCATCTTAGGTAGGTATAGTACCTCATGTTTCTATTAAGCTTTTCGACGTAATCAATAAATAAGCTTATGTGTATATGTCTCTAATTTTAATTGTAGGACGCATGCAAATTTGTAATTGGTTCCAAATTAATGACATGTCAATTTGTGATTGGCCGAACTTTTCAACCAATAGAAACAATTTTATTGATAAGTGCAAAGAAGAAAACAAAGATAGGGAATTATAGAATGAGTGACGGGAGGGATGGAGATCGAGAGAAGCAGATGCTGAGTTGTTCAGGTTTAGGCATGGGTTTACTCAAAGAAAAACGTCCAAATTTCTCTCCTTGGATTCTTAAATATACACATATATATATTTTCTAATTTCTTATTCTGCAAGCTATTAACTATAAAGATAATTTCGACTTGGCAAAATGAGTTAGCCCTGTTCCCTTTTTTCAGGTCGGAGGTCTAGTTTTGCCTCAGTTTTTAAGGTTATATTGAAATATAAAAAAACAATAACAATAATTTTGAATTCAATTTTCTAACCAATTAAAATCAACCTCGCACATTTTAGGAGCCCTCGGTTCTATACGGTCATCTTTAGGTTATAGAAGGTTTAATTTTATTGGCATGACGGTCATAAGACAATATCCATTATTGGCATTTCGTTCAATGGTTCTATATTTATTAAATATAACTTACCGAGTGTTATGCTGTTTTCAGAAATGTACTTGTCAAAGGTGGGGACCTGCCTGATTCATTGGATGCGGTTGATATATTCTTTGATGGTAAGTACGTATTTGCATACTTTTGACAATTTGATATTATTTCTTTTTGTTACGAAGGAAACATCCATTGAATCCAAAATTTAAAATAATTTTCACAGGCAAGGATTTGCATGAGCTACGATCTTCGCGCATAAAGTCTCGCAACACTCATGGTACTGGATGCAGCTTAGCATCGTGCATTTCAGCTGAACTTGCTAAAGGGTCTTCAATGTTCTCAGCTGTTAAGGTAATTGTATATGGGTCTCTTCTTTAATTTACTAACTTCAGCCGCAAGAAGATCCAAGTCTTAAGGGTTCACAATACAACTTCCTATTGAATGGTTAAATAAATCTAGTTATAATAATCTAGCTATATTAATAGCTCTAATTTACCGTTAGGGTGGGCCATGAGCATCATACAAGGAAAAATAGCATCGACAATCTTATTGATGAAAAAGGAGAGAAGAAAATAATAGAAACGAACATCTTGTCATGATATACTTCTTTTAACTCATTCATCAAAATATGATTGACTCTCAATTCAATTAGGAGTTTTGAATTCTTTGATTATTTGTCTTCTTTCTTTTTGGATGAAAAGTCATCATTCTCACTCCATATCCATTTGATGACTGAATCACGCCAATAAGCTTTGTTGTTGATCAACTGACTTGATGACTGCCTACAACTAAGGTCTTACTTATCTGGATTTTTTACAGGCAAGCAAACAGTTCATTGAAAGAGCATTGAGGTACAGCAAGGACATTAACATTGGACATGGACCTCAAGGCCCATTCGATCATCTATGTCGTCTCAAGAGTCGAGAACAAAGTTCCTATAGTCAGGGGTGTTTCAATCCAACTGACTTGTTCTTGTATGCTGTTACGGACTCGGGTATGAATGAGCGTTGGGACCGTTCTATCACTGATGCTGTTAAAGCTGCATTGGAAGGAGGTGCTACTATTGTTCAAATAAGGTTTGCCTAATGTCTTGTATAAGTTAATATATAATTTAAGTCATTACTTCAAAATGCATGGAATTCCTTGTTGCATTTTAACATCTCCTCTCAACACCTCAGTTCAAAGGTTAAATGGCTTTAGAACTTAACCCATAAAGTGTGGTTTGGATCCTCCTTAGGCGAGCGGTAGTGAAGTTATCTAGATTTGAATATCTTAGGCCTTGAACCATTAAGGTCCCGTTTGGTAGCCATTTTGTTTTTAAAAATTAAGTCTATAAATGCTATTTTGATGAAGAAAGAACACATGATCTACGTACAAAAAGAAAAACCACGATATAGATGTTTCTTATTATTTTCTGATGATAATAAAAGTACCAAGGTGAAATATTTATAGGCAACGTGAGCCTAATTAAAATAATAAAAAATTAGAGCAAAGTAATTCTCAACTACTCTTGGGCTTTCAACATGACCCAATGTCACGTACCTTGAAAAACACCTTAAAATTTACGCCGGAACGTGTCACAAGGCTCCTATAGCAAAGCCAAGGGGGAGCCTGGCATGCCCGCACAACACCCCAATGAGGCCATGGGACAGCCCACATAGGCCCTCGACACGCCCATGACATTGGCGTGTGCATACGGAAGGCAGCCCACGAGCCCCGCATGCATGTCAAGACATGTGCCCATGACAGCCCCATGAGGCGGGCTACACGCACAACCCACGGGCACCAACGGATGCACACCAACGCCCGGCACCAGCGCTACCCAGACAGCCCATGCGCACAGGCAGCAGCGCATGGCATGGCGGCCAGCCCAGCATGCGCGCATACAGGCGCGCACAAACTGTCACGTGGTTATTTTTAACACCTAACCAACGTGTGACACTTGGCTAGAAATTATGCCTGCGTTAGCCAAGTAAGCCTTTCAACAATATTAGTGCACCAAGCACTATGGGTAAAATAACGCAGCGGAATTAATTTAGTGTAAAACAGCACTTAGAATTTATGGAAGGTTAGGCAAGAGAGAGAATATGGGTTTGGAAATGAGAGCTTGAGGTAAATGTCTTGCTTTACTTATATTACCAAGGATTTACAAGGATTCCCAGGAGTTTTAGGAGTTCCAGGAATTGAGTACAAATGAGACCCCCTTGGGGTAATTATACAAAGCAATGGCCGACTTTGAACCTATACAACCGACTTAAAAGGTCGACAAAAACGTTCTAGAGAGTTCCCGGGTTTTAATGACGGTCAGTCACCGACATGGCACTGTTCCCGACCGACATGGAAGGTTCCAGAAGCGTCTGGAGAGGGTCGGAGCGAGTGCGCGGGTCGCCGTTACGTCTGTCAGGCGCGTGCGCATTCCGCCCGTTACGGTGTCGAACGTCCGTCATCGAATCTTCTAGAAACGTCTCGGCTCTTCTCGGTCCTTTCTGGTCCTTCTTGGCCATGGGCGCACGCGCAAGGTGTCGCGCGCGCCGTCGCGTCGCGGCTGCGCAGCGGCGTTGCGTGCGGCATATGTCCATCCATCTTGGCGCGCGCGCAGGCGTTGTCTTTGCGCGCGCGTCCGACATCCGCATGGAGGCCTTGGCCTGCATATGTGTATGTTACATCTGCATGGGCGTCGCCTTGCACGCCTGCACGCGCACACCATCTGCATCTAAATGGGGCGCACGCGCATCCTGCCGCGTGTCAAGGGCATAATTGTCCAAGGTATTATTTACCGAGGGCCGTATGTTCGAATACCCCCAAATCACTTTATCTTTATGTCTTGAAAGTAGTTTAGGTTAATTCTTTCGTTTAGGTGTCGCCGAGTCACAGTGTGACATTTCGATTTTTTCATATGCAAGCCTGCCAAGGCCAAAAATCTCAATATGTACTATATGGGGTACATGACTAGTCCGACCCTCGCTCAAGCCTTGTCGCACTCTTTGCATGTTAAGCTATTTTCCTTGCAATTGACAGTCTTCAATGCTTTCTTTATGACGTTTTCAACTATTTACTTTCTCTCTCCCGTTTTATAAAAACATTTTCCCAAGAACGGGGAGGGAGGCTACGTCATACTGCGAGTTTGTCCGCGGATTTCGATTTTTGAACGGCTGACTTGCTGATCGAGCTAAGCGAGTGCCGCGCACAATCGTGTTGAGAAGTTACGATTGTGACAAGTGGTATCAGAGCGAAATTGGCTAATTTGACGATAAGATCGAAACATGTCGTCGTCGAATCCATCGGGCAAGGCCCAGAAAGACCGACTAGTAGAGCTAGAAGAGCATATGCTCTACCTAGTCGAAGTTCCCGACTCCATCCGCTACTTGGAGTCTCGTCTCGATGAAATTTCTGAGAAAACTGATGCAATCGATGCGGTAGCTGGCCGTGTCGAAGCGTTGCCGATACAAGAGTTGTTGGCAAGAGTCGACAACAGAAGTAGAAGCTAAACGTTTGAATCTCCGCTGGGAGAAGGATGCTGGAAGAATGAAAGCCGTGAATTCTGCTGCCCTATCTATCATCGGACTAGTGAAACGAACGATGATAAGATTGGGAGAATGGAGTGGCCTCGTAGACTTTGTAGTAGTAAAAATGGATGACTTTGATGTGGTACTGGGAATGGAGTTCCTACTTGAACATCAGGTAATCCCAATGCCTTTGGCCAAATGCTTGGTGATCACTGGACCTACACCCTCGGTTGTACAGACTGACCTACGTCAACCAGATGGATTGAAAATGATCTCGGCCATGCAATTAAAGAAGGGTCTCTCTCGAGACGAACCAACATTTATGGCCATCCCACTCAAATCGTCAGAGAACTCAGGGGAGACAGTCCCTAAGGAGATCATGCGCGTGCTAGAGAAATACCGTGATGTGATGCCCGATAGTTTGCCCAAGTCTTTGCCACCTCGAAGAATGATTGATCATGAGATCGAGTTAGTGCCAGGGGCAAAACCGCCTGCGAAGAATGCTTATCGTATGGCGCCTCCGGAGTTAGCTGAACTTCGGAAACCGTTAGATGAACTACTGAATGCAGGGTTTATCAGGCCTGCAAAAGCTCCGTATGGGGCCCCAGTTCTTTTCCAAAGAAAGAAAGATGGGAGTTTACGACTGTGCATTGATTATCGTGTCCTAAATAAGCTCACAGTCCGTAACAAGTATCCACTTCCCATAATTACTGACTTGTTCGACCGCTTACATGGGGCAAAGTATTTGTCAAAGTTAGACTTGCGGTCGGGATACTACCAAGTGAGAATTGCAGAGGGAGATGAACCGAAGACAACCTGTGTCACCCGATATGGTGCGTTCGAATTCCTCGTAATGCCATTTGGTCTCACCAATGCCCGCATACCTTCGCGTTGATGAACTGGTCTTCCACGAATATCTCGATAAATTCGTAGTAGTCTACTGGATGATATAGTGGTCTAAAGTACGACCATGAAGGAACATAGAGACCACCAAAAGGTTTTTCGAAATTGAAGGAGAATCAACGTAAAGAAGAAAAATGCTCTTTTGCACAAGAGCGGATAAACTTCTTGGGCCATGTGATAGAGTGTGGCGGATGGAATGGAAGAAGGGAAGATTGCGATACGCGGCGAGCAATGCGAATCGTCTCGAGTTACGCTCCTTCCTCGGGTTGGCATTACTTATCGATTTGTCGAGGATTCTCAAAGCGAGCAAGCCCGTGAGCTGCTGAAAAAAGGCGTTCACTGGAATTGGGACCCCGAGTGCCAAGCCGCCTTCGACGGCCTAAAACAAGCCTTGATGGAGGGGCCACTTCTAGGGATTGCGGATGTGACCAAACCTTTCGAAGTCGAGACAGATGCGTCTGATTATGCATTGGGGGGTGTGCTCCTACAGAATGGGCACCCGATCGCATACGAAAGTCGAAAATTGAATGCAGTCGAAAGGAGGTATACTGTGTCCGAAAAAGAAATGCTCGCAGTAGTACATTGTTTGAGGGCCTGGAGACAATACCTACTAGGGTCGTCGTTTGTAGTGAAGACGGACAACAGTGCAACCTGCCACTTCTTTACCCAGCCAAAGTTGACTTCGAAACAAGCAAGATGGCAAGAATTTCTAGCCGAGTTCGACTTCGAATTTGATCACAAGAAGGGGTCGAGCAACCAGGCTGCGGATGCCCTAAGTCGAAAACAAGAACATGCAGCCATATGCCTGTTAGCTCACCTCCAGGAGAGCGAGATTGGTGGGTCGGTCAGAGACACCTTGAGAGAGTTCCTACAGAAAGATCATGCCGCTCAGAATGCCATGAATTTAGCGAAGGCGGGCAAAACACGACAGTTTTGGGTCGAGGAAGGCTTGTTAGTCACAAAGGGCAATCGACTATATGTTCCAAGAGCAGGGGACTTAAGGAAGAAATTGTTGTACGAGTGTCACGACACTCTATGGGCTGGCCATCCCGGATGGCAGCGGACGTACGCCCTGTTGAAGAAGGGCTACTTTTGGCCGAATATGAGAGATGATGTCATGTAGTACACTAAGACGTGTCTAATCTGCCAACAAGATAAGGTAGAGAAAGTGAAGATTGCTGGACTTCTTGACCCTCTACCGGTTCCAACAAGACCTTGGGAGAGTGTCTCTATGGACTTCATCATCCATCTCCCTAAGGTAGGCGACTTTGAAGCCATTTTAGTCATCATTGATCGTTTTTCAAAGTACGTCACCTTCATCCCCGCCACCAAGCAGTGTTCAGCAGAAACAATAGCTCAATTGTTCTTCAAGCATGTTGTTAAATTGTGGGGAGTCCCGACAAGTATAGTGAGCGACAGAGATGGTAGATTCATTGGCTCCTTCTGGACGGAGTTATTTTCCTTCTTGGGGACGAGTCTGAACATATCCTCAAGCTACCATCCCCAAACTGACGGCCAGACTGAGCGATTCAACTGCATGCTCGAGGAATACTTGCGCCATTTTATAAACGCAAGGCAAAAGAATTGGGTCCAGCTGTTGGACGTAGCCCAATTCTGTTTCAACGCTCAGACAAGTTCATCTACAGGGAGAAGCCCATTTGAGATTGTTTCTGGGAGGCAACCAGTACTGCCATACCTTGTTGATCATCCTTTTGCAGGGAAGAACCCTCAAGCCCTCAATTTCACGAAGGAGTGGAGACAGACAAACGACATCGTCCGAGCGTACTTAGAAAAAGCATCGAAGCGGATGAAGAAGTGGGCAGATAAGAAGCGACGGCCCCTCGAGTTTCGAGCGGGAGACCAGGTACTCATTAAACTACGGCCAGAGCAAGTCAGGTTTCGAGGACGCAAAGACCAACGTCTCGTCAGGAAGTACGAAGGACCAGTCGAAGTGCTGAAAAAGGTAGGGAATACTTCTTACAGAGTAGCGTTGCCCACATGGATGAAAATCTACCCAGTAATTCATGTTAGCAACCTGAAGCCGTACCACCAAGACACGGAAGACCTGCAGCGAAATATCATAACTCGCCCAATTATAGATCTTAGTCAGAAGGAAGACAAAGATGTTGAAGAAATTCTGGCCGAGCGAGTAAGGAGGGGCAGAAGACCCGCACGAAGGATCCACGAGTATCTGGTAAAGTGGAAGAACCTTCCTGTGGAGGAGACCAGCTGGGAACGTGTCGAAGATCTTGAAGCGTGGAAGCAGAAGATAGAAGATTTCAAGCTTCGTCAGTTGACGGGGACGTCAACCATTTACGTGGGGGAGAATGTCAAGGGCATAATTGTCCATGGTATTATTTACCGATGGTCGTATGCCCGAATACCCCCAAATCACTTTATCTTTATGTCTTGAAAGTAGTTTAGGTTAATTCTTTCGTTTAGGTGTCGTCGAGTCATAGTGTGACATTTCGGCTTTTTCATATGCAAGCCTGCCAAGGCCAAAATTCTCAATATGTACTATAGGGGGTACATGACTAAGTCCGACCCCCGCCCAAGCCTTGTCGCACTCTTTGCATGTTGAGTTATTTTCCTTGCAATTGACAGTCTTCAATGCTTTCTTTATGACGTTTTCAACTATTTACTTTCTCTCTCCCGTTTTATAAAAACATTTTTCCAAGAACGGGAAGGGAGGCTACGTCATACCGCGAGTTTGATCGCGGATTCCGATTTCGAACGGCTGACTTGCTGATTGAGCTAAGCAAGTGCCGCACAATCGTGTTGAGAAGTTACGATTGTGACACGCGTGACACCCTGCGCGCGCGCCAATGGCCGAGAAGGACCGAGAAGAGCCGAGACGTTTCTAGAAGATTCGATAACGGACGTTCGACTTCGTAACGGGCGGAACGCGCGCGCGCCTGACGGATGTAACGACGACCCACGCGCGTGCTCCGCGCCTCTCCAGACACGCCCAGACGCTTCTGGAACCTTCCATGTCGGTCGGGAACAGTGCCATGTCGGTGACTAACCGTCATGAAAACCCGAGAACTCTCTAGAACGTTTTTGCCGACCTTTTTAGTCGGTTGTAGGGGTCCAAAGTCGGTCATTGCTTTGTATAAATACCCCAAGGGGGTCTCATTTGTACTCAACTCCTGGAAACTCCTGGAACTCCTAAAAACTCTTGGAAATCCTTGTAAATCCTTGATAATATAAGTAAAGCAAGGCATTTACCTCAAGCCCTCATTTCCAAACCCGTATTCTCTCTCTTGCCTAACCTTCCATAAATTCTAAGTGCTCTTTTGCGCTAAATTAATTCCGCTGCGTTATTTTACCCATAGTGCTTGGTGCACTAATATTGTTGAAAGGCTTACTTGGCTAACGCGGGCATAATTTCTAGCCAAGTGCCGCACGTTGGTTAGGTGTTAAAAATAACCACGTGACAGTTGGTATCAGAGCAACGATCTCTGAGGGCTATAACCGATACCAAGCTACAGTTGGTGCCAAAGCGAATCGGTCCCTGTGGAGTAATTGATACCGAGCGATAATTAGTATCAAAACAAACCGATCCCTGAGGAATACCTCTTTGACGAATAGGTGGTATTTGGATTTCAAGATTTGATAAATCTTGAAGGATTGTGCTTAGGGTGTATCGGAGCAAAGTTGTAAGGACCTAGGTACCCCTAAATTGTTTTACACTACGTTTTGACTCAATGGCATCCGTGGAAGGACACGAGTCGCCCGTTGAGCAAGTCGTTGAAGTGCCAACGACAAGAGGACGTGCTGCACGATCCCGAAAGACGACGGCGACACAGGAGAATGTCGAGGAGCGTCTTACTAAATTGGAAGAGGGATTGAAAGACGTGCAGACCGAAGTCGGCAAGATGAGCGGGTTAATGGACGAGCTAATCCAGGATAACGTGGAGATCTCGAATGCCGCCAAGGAAATGATCAAAGACCTTGGAGACTCACACGGTAAGGAGATGTATGATCTTCTTGTTGAGGTAACTAACTTACGAAAGTTCGTGGAAGAAGAACTTCACGCCTTGCGAAAGGACGTCGACGAGGTACGTGCCGAATGACACTCTAGACATGCTAGCAATGGTAATGCATCCACTAGCACATCATGCACCATGGTAGGCACTCACAACATCAAAGTGCCGAAACCCGACATGTATAATGGCACGAGGAATGCCACGATGGTGGAAAATTTCCTATTTGGTCTTGAACAGTATTATAAGGCTTTAGGAATCATCGACGATGGTGCCAAAATTGCAATCGCCCCAAATTTCCTACGTGAGTCGGCACAATTATGGTGGCGTAGGAAGCATGCGGAATGGGAGAAGGACCGCACGATCTTGCAGACGTGGGAACAATTCAAGGCAGAACTTCGAAAGCATTTTCTTCCCCACAATGCGGATATGGAGGCGCGAGGTAAGTTGAGGAGATTAAGGCAAATCGGTAGCATTCCCGATTACATAAAGGAGTTCACTACTCTAATGTTGGAGATAGAAGATCTATCGGACAAAGATGCTCTCTTTCATTTTAGAGACGGTCTTAAAGATTGGGCGAAGATCGAGCTCGATAGACGCAATGTGCGAACTCTTGATGATGCTATTGTCGCAGCTGAAGCGCTTATCGACATCTACTCCAAGAAAAAGAAGAGGCGTGTAGATGAAGGAGAAGCGTTCGAACCAAAGAAGGAGGGCAACTCTAAGCAAGACGGGAAGTATGGAAAAGCCACATCGGACAAGAGATTGTCCAAAGAAGAACAAATTGAGTGCAATCGTGGCCAATCCACGGGAAGAAGAGGAAGTACCCATAAAAGGGACGGGCACAGTTAGGTGCAATCCGATACCTAAGTGCCATGAAGAGTGAATCCTTTAAAGAACCTCAAGAAGGAGGTTGGTTGTATGTGGATGCCACGTTCAACAACAAGGCTGCCTTAGCGATGTTAGACGCTGGCGCAACCCATAATGTAATGGATCCTGAAGAGGCAAAGCGGCTAGGCCTCAGGACCACCAGGAGAAGCGACACTGAGATAAAGATCATCAGTGCCAAATCCATGAAGAACATTGGATGGGCTCAGGATATCAAGACAAGGGTAGGAGGATGGCAAGGTAAGCTCGATTTCCTCATAGCCCCTCTTGACGATTGTAAGATAATCCTAGGATTGGACTTCGTTGATACAGCCCATGCCACAATCAACTTCATGAAGAAGACCCTAGATTTTACCCACATAAGGGAGGAGGCGCCACTTAAACGCATGGGAGCAATGAGAGAAGTGAGGCTTAGTGCCATGCGAGTTAGAAGACGCTATAAAGGAAAAGGGTCACGAAAGTCCAAGAACGCAACAAGGACGTTGCGAATTGGAGTGGGGGAGGGTGTCACGTACCTTGAAAAACACCCTAAAATTTGCGCCGGAACGTGTCACAAGGCTCCTATGGCAAAGCCAAGGGGGAGCCTAGCATGCCCGCACAACACCCCAATGAGGCCATGTGACAGCCCACATAAGCCCCCGACACACGCCCATGACATTGGCGTGTGCCTACGGAAGGCAGCCCACGAGCCCCGCATGCAAGTCAAGACATGTGCCCATGATAGCCCCATGAGGCGGGCTACACGCACAACCCACGGGCCTCTCATTATCATGACTAGGGGACTCCACATTGTTCATGATATGCCATTGTTTCTTACAATGCTCACAGATAGGGATTGGTTTCCCATTGTGCCTCTCATTATCATGACTAGGGGACTCCACATTGAAGCATCAGAATCAATGGTGGGGGTTGTCAGACTATTCATAACACTGGTATGATCCTCCTCATGGTGAACCTTAGAACAAACCTCTATCAAGGAGGGTATAGGTCTTTGGCCCAGTACACGTTCTAGACAATATCAAACTTAGGGTTGAGACCAACTAGAAAATCATAAATCTTGTCAACTTCCTCGATCTTGGAGTACTGTATGCCATTGCTAGGACAATTCCAAACAATTTCTCTGCATAGGTCCATTTCTTTGGAGAGTTAAAAAATGATGTCACATTCATTGTCCCCTATTTGCTTTCATGAATGTGTTTTTGTAGAGTATGTAAATGTGAGGCATTCTGACGCTTGGAATTCAATTTTTGAGTCGTGTCCCAAATATCCTTAGCAATTGCTGCATACGGTAAGGGCTTGCGAATCTGTGGTTCCATACTATTAATCAATGAAAACTGAATAAGAGATTCCTCCCTTTTCTAGTATCGTTTCCAAGGGTCTCTTGACGGAGGACGAGGTATCTCACTTGTCAGAAAGTCAAACTTTTGGTGCCTTTCAAGAATTATTTTGGGACCATAAAGAATAGTTTTGGTCATTAAGCTTTTTCCCTGAAAAATATCTTGCTGATTGTGCACTATATTAGACATATAGGAAGAGGATAAAGAGAGGAGCGATGTTTACCAGATTCTCATAATACATCAGTGGGACTATATGAAGCCAGGACTAGGGTTGGTAGAAATGGCTCCTAATGTCGTTTCATTGCCACAATTTGTTGTCAATGCATGTTCACTTGGTGCTGCCCTATTCTCGACGAGAGCTTGTACATGAGGATTTGATTATGTCGAGGACTCGCTATCCTCAGAAGTTGAATGGATATGAGCAGCATATCAGTGGGTGACAATTGGACATTAGTCTGTTGTTGGAGGTGACTGAGTAGTAGCTCGTGTAGAAGCAGCGGTGGCATATGCAGGAAACCCAAAATCCAATTGCGACTGGGTTGTTGTCAGGTGGTGTTTCAGTAGGGGTTCATCTCGTGTGGCTGAGGAGGGAGGCTAGTTGGAAAGCTATAGCTGAAAGGTGGTGGTGGCCGGACCTCTTGCGGCTGGAAGAGGAAGTTTGCTGAAGTGCTGCGGTTGAAGGGAAGGGTTGGCTGGTAGGTTCTATAAGGCGGCAAAATAGATCCTTCATGGTGGCGTTCATCCAAGCATCGACAGCAGTAGCGATGGCGATGCTGATCCCGTGGTTGTTTCCCTCGTTTGAGTTCCATCAAGTGTCAAGTCGTCTAGGGGTTCATCGATATCTCGCTCTGATACCATATCGAAACATAAAGACGAAGAAGACGAGGTCTATGTGGAAACCCTAGTAAATGGAGAAAGACCAAGGTATGAATGTTTCTTATTATTTTCTGATGATAATAAAGGTCAAAAGAGAAATATTTTTAGGCAACACGAGCCTAATTAAAATAATAAAAAACTAAGGCAAAGTAAATTTCAATTATTCTTGGGCTTTCAACATGACCTAAGCCCACTATTTCTAACAACACTACTTTCACCTCCAACTTTCTTTATTACCATCATTCTACCAACGGTTTAAAAAAACCAATCCAAAATTTTAAAACTAAAAGTAGCTTTCAAAAAGTTGTTTATGGAATTGACTAAGAATTCAATCGTTGTATTTAAGAAAAATGCAAATTATTGTAAGTTAGGGAAATGAAATAGACTTAATTTTAAAGAACAAAAACCAACAACAAGATGTTACCAAATGGGAACTTTTTTTTAGTTTTTGAAACTAAGCCTATGAAGAGCCTTTTACCCTTTTCACCTCTAAATTTGTTGCTTTTTAATCTACTTTCTGCCGTTGTTTTAAAAAAATTGAAAAGAACAAAAAAAAAAAAGTTTTTAAAATTTTGTTTTTTAAATTTAGTTAAGAATTTAATTCTAACTCGAGAAAGATGAAAACCATGGTAAAATATTTTATTGAGAGTAAACAGACTTAATTTTCGAAAACCAAAACCCAATTGATTACCAAGCAAGGCTTAATTAACTTTTGGTAGAAGTTAAATACTAGGCATAGTTTTGTAAACATTTATTCAACTCTTGATTTTCTTCCAATGTAGTTCTCTCTTATTCTTATGAACAGAGAAAAAGATGCTAAAACTCGTGATTTCTTGGAAGTAGCAAAGTCATGCATAAAGATTTGTCACGCACATGGAGTTCCATTGTTGATCAATGATCGTATTGACATTGCACTTGCGTGTGATGCTGATGGTGTACACGTTGGTCAGTCTGATATTCCTGCTCATGAAGTTCGCCGCCTTCTTGGCCCCAACAAGGTCATCGGTGTCTCATGCAAGACAATGGAGCAAGCTGAACAGGCATGGATTGATGGTGCGGATTACATTGGGTGTGGCGGAGTTTATCCCACCAACACAAAAGCAAACAATCTAACCGTTGGGATTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAATTGCCAGTGGTAGCAATTGGTGGTATTAATCACACTAATGCAGCTGCTGTGATGGGAATTGGTATCCCAAATCTTAGAGGTGTTGCTGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGCGGCCTCCAAGTTACATGCTACTTTAGTGGAGGCTACAACATCAAATGTTTGAATAGTTTTGTAATGCTTTGTTTTCTGTAGAAAGCCTTTTGAAATAAAAGTAATTGTCACAAAGTGTTTTTGAATTAATAATATATATATATATATATATGTCA

mRNA sequence

TCAGCGCCCCCGTCACACACTGCATTCTCAGCTTCTCCATTCTCAGCCGTCCGACGAAATTTCCGCCTCCTCCTCCCTCTCACACTTTGTCGCCTCCTTGAGCCATTAGAGTTACATCTCTGTTTTGTTTCTCAGAAACGCAGTTGTTCAACCCCTAGCCGGCGCCGTCGGTGAGATGGTGCCGCTGCCCCTCATTTCTCAGATCCCTAAGGAAGAAATGGTTGTAGCTTCAAGTGATCGATATGAGACGCGGATTCCGCATGTGTTGAGTGTTGCTGGTTCTGATTCGGGAGCAGGGGCTGGAATCCAAGCAGATCTTAAGACATGTGCTGCTCGTGGAGTGTATTGCTCCACTGTGATAACTGCTATTACTGCACAGAATACTGTGGGGGTTCAGGATGTAAACATTGTTCCGGAGGGCTTTGTTTCAAAGCAGCTGAAATCTGTTCTCTCTGATATGCAAGTGGATGTGGTGAAAACAGGAATGCTACCTTCTACTGGCATTGTTCAAGTTCTACATCAGTGCTTGAAGGAGTTTCCTGTTCGAGCTTTGGTGGTTGATCCTGTCATGGTGTCTACTAGTGGAGATGTTCTCGCTGGTCCTACTATTATTTCAGTGTTACAGGAAGAGCTTCTACCAATGGCGGACTTGGTAACTCCAAATTTGAAGGAAGCATCTGCCTTACTTGGTGGTATGCCACTTAAAACAATTTCTGACATGCGTCATGCTGCAACGTTAATCCATCAGATGGGATCAAAAAATGTACTTGTCAAAGGTGGGGACCTGCCTGATTCATTGGATGCGGTTGATATATTCTTTGATGGCAAGGATTTGCATGAGCTACGATCTTCGCGCATAAAGTCTCGCAACACTCATGGTACTGGATGCAGCTTAGCATCGTGCATTTCAGCTGAACTTGCTAAAGGGTCTTCAATGTTCTCAGCTGTTAAGGCAAGCAAACAGTTCATTGAAAGAGCATTGAGGTACAGCAAGGACATTAACATTGGACATGGACCTCAAGGCCCATTCGATCATCTATGTCGTCTCAAGAGTCGAGAACAAAGTTCCTATAGTCAGGGGTGTTTCAATCCAACTGACTTGTTCTTGTATGCTGTTACGGACTCGGGTATGAATGAGCGTTGGGACCGTTCTATCACTGATGCTGTTAAAGCTGCATTGGAAGGAGGTGCTACTATTGTTCAAATAAGAGAAAAAGATGCTAAAACTCGTGATTTCTTGGAAGTAGCAAAGTCATGCATAAAGATTTGTCACGCACATGGAGTTCCATTGTTGATCAATGATCGTATTGACATTGCACTTGCGTGTGATGCTGATGGTGTACACGTTGGTCAGTCTGATATTCCTGCTCATGAAGTTCGCCGCCTTCTTGGCCCCAACAAGGTCATCGGTGTCTCATGCAAGACAATGGAGCAAGCTGAACAGGCATGGATTGATGGTGCGGATTACATTGGGTGTGGCGGAGTTTATCCCACCAACACAAAAGCAAACAATCTAACCGTTGGGATTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAATTGCCAGTGGTAGCAATTGGTGGTATTAATCACACTAATGCAGCTGCTGTGATGGGAATTGGTATCCCAAATCTTAGAGGTGTTGCTGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGCGGCCTCCAAGTTACATGCTACTTTAGTGGAGGCTACAACATCAAATGTTTGAATAGTTTTGTAATGCTTTGTTTTCTGTAGAAAGCCTTTTGAAATAAAAGTAATTGTCACAAAGTGTTTTTGAATTAATAATATATATATATATATATATGTCA

Coding sequence (CDS)

ATGGTGCCGCTGCCCCTCATTTCTCAGATCCCTAAGGAAGAAATGGTTGTAGCTTCAAGTGATCGATATGAGACGCGGATTCCGCATGTGTTGAGTGTTGCTGGTTCTGATTCGGGAGCAGGGGCTGGAATCCAAGCAGATCTTAAGACATGTGCTGCTCGTGGAGTGTATTGCTCCACTGTGATAACTGCTATTACTGCACAGAATACTGTGGGGGTTCAGGATGTAAACATTGTTCCGGAGGGCTTTGTTTCAAAGCAGCTGAAATCTGTTCTCTCTGATATGCAAGTGGATGTGGTGAAAACAGGAATGCTACCTTCTACTGGCATTGTTCAAGTTCTACATCAGTGCTTGAAGGAGTTTCCTGTTCGAGCTTTGGTGGTTGATCCTGTCATGGTGTCTACTAGTGGAGATGTTCTCGCTGGTCCTACTATTATTTCAGTGTTACAGGAAGAGCTTCTACCAATGGCGGACTTGGTAACTCCAAATTTGAAGGAAGCATCTGCCTTACTTGGTGGTATGCCACTTAAAACAATTTCTGACATGCGTCATGCTGCAACGTTAATCCATCAGATGGGATCAAAAAATGTACTTGTCAAAGGTGGGGACCTGCCTGATTCATTGGATGCGGTTGATATATTCTTTGATGGCAAGGATTTGCATGAGCTACGATCTTCGCGCATAAAGTCTCGCAACACTCATGGTACTGGATGCAGCTTAGCATCGTGCATTTCAGCTGAACTTGCTAAAGGGTCTTCAATGTTCTCAGCTGTTAAGGCAAGCAAACAGTTCATTGAAAGAGCATTGAGGTACAGCAAGGACATTAACATTGGACATGGACCTCAAGGCCCATTCGATCATCTATGTCGTCTCAAGAGTCGAGAACAAAGTTCCTATAGTCAGGGGTGTTTCAATCCAACTGACTTGTTCTTGTATGCTGTTACGGACTCGGGTATGAATGAGCGTTGGGACCGTTCTATCACTGATGCTGTTAAAGCTGCATTGGAAGGAGGTGCTACTATTGTTCAAATAAGAGAAAAAGATGCTAAAACTCGTGATTTCTTGGAAGTAGCAAAGTCATGCATAAAGATTTGTCACGCACATGGAGTTCCATTGTTGATCAATGATCGTATTGACATTGCACTTGCGTGTGATGCTGATGGTGTACACGTTGGTCAGTCTGATATTCCTGCTCATGAAGTTCGCCGCCTTCTTGGCCCCAACAAGGTCATCGGTGTCTCATGCAAGACAATGGAGCAAGCTGAACAGGCATGGATTGATGGTGCGGATTACATTGGGTGTGGCGGAGTTTATCCCACCAACACAAAAGCAAACAATCTAACCGTTGGGATTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAATTGCCAGTGGTAGCAATTGGTGGTATTAATCACACTAATGCAGCTGCTGTGATGGGAATTGGTATCCCAAATCTTAGAGGTGTTGCTGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGCGGCCTCCAAGTTACATGCTACTTTAGTGGAGGCTACAACATCAAATGTTTGA

Protein sequence

MVPLPLISQIPKEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAALEGGATIVQIREKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV

Homology

[Close]

BLAST of MELO3C022605.jh1 vs. NCBI nr
Match: XP_008460243.1 (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 1040 bits (2689), Expect = 0.0
Identity = 534/535 (99.81%), Postives = 535/535 (100.00%), Query Frame = 0


Query: 1   MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA 60
           MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180
           STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE
Sbjct: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180

Query: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300
           RIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD
Sbjct: 241 RIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300

Query: 301 HLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAALEGGATIVQIRE 360
           HLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAA+EGGATIVQIRE
Sbjct: 301 HLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAAVEGGATIVQIRE 360

Query: 361 KDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG 420
           KDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG
Sbjct: 361 KDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG 420

Query: 421 PNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480
           PNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV
Sbjct: 421 PNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480

Query: 481 AIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV 535
           AIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV
Sbjct: 481 AIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV 535

[Close]

BLAST of MELO3C022605.jh1 vs. NCBI nr
Match: XP_008460244.1 (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 1000 bits (2585), Expect = 0.0
Identity = 514/515 (99.81%), Postives = 515/515 (100.00%), Query Frame = 0


Query: 21  MAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAIT 80
           MAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAIT
Sbjct: 1   MAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAIT 60

Query: 81  AQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRAL 140
           AQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRAL
Sbjct: 61  AQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRAL 120

Query: 141 VVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAA 200
           VVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAA
Sbjct: 121 VVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAA 180

Query: 201 TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISA 260
           TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISA
Sbjct: 181 TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISA 240

Query: 261 ELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNP 320
           ELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNP
Sbjct: 241 ELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNP 300

Query: 321 TDLFLYAVTDSGMNERWDRSITDAVKAALEGGATIVQIREKDAKTRDFLEVAKSCIKICH 380
           TDLFLYAVTDSGMNERWDRSITDAVKAA+EGGATIVQIREKDAKTRDFLEVAKSCIKICH
Sbjct: 301 TDLFLYAVTDSGMNERWDRSITDAVKAAVEGGATIVQIREKDAKTRDFLEVAKSCIKICH 360

Query: 381 AHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWI 440
           AHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWI
Sbjct: 361 AHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWI 420

Query: 441 DGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPN 500
           DGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPN
Sbjct: 421 DGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPN 480

Query: 501 LRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV 535
           LRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV
Sbjct: 481 LRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV 515

[Close]

BLAST of MELO3C022605.jh1 vs. NCBI nr
Match: XP_038876926.1 (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Benincasa hispida])

HSP 1 Score: 989 bits (2558), Expect = 0.0
Identity = 503/535 (94.02%), Postives = 523/535 (97.76%), Query Frame = 0


Query: 1   MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA 60
           MVPLPLISQIPKFNQVSRFC+AMKK EE VVASSDRYE RIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVPLPLISQIPKFNQVSRFCVAMKKHEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVS+QLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSEQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180
           STGIV+VLHQCLKEFPV+ALVVDPVMVSTSGDVLAGPTIISVLQ+ELLPMADLVTPNLKE
Sbjct: 121 STGIVRVLHQCLKEFPVQALVVDPVMVSTSGDVLAGPTIISVLQKELLPMADLVTPNLKE 180

Query: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300
           RIK+RNTHGTGCSLASCI+AELAKGSSMFSAVK SKQFIERAL+YSKDI IG+GPQGPFD
Sbjct: 241 RIKTRNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALKYSKDIGIGNGPQGPFD 300

Query: 301 HLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAALEGGATIVQIRE 360
           HLCRLKSREQSSY QGCFNP DLFLYAVTDSGMN+RWDRSI+DAVKAA+EGGATI+QIRE
Sbjct: 301 HLCRLKSREQSSYRQGCFNPADLFLYAVTDSGMNKRWDRSISDAVKAAVEGGATIIQIRE 360

Query: 361 KDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG 420
           K+AKTRDFLE AKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG
Sbjct: 361 KEAKTRDFLEAAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG 420

Query: 421 PNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480
           PNK+IGVSCKT EQAEQAW+DGADYIGCGGVYPTNTKANNLTVGI+GLKRVCLASKLPVV
Sbjct: 421 PNKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGIEGLKRVCLASKLPVV 480

Query: 481 AIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV 535
           AIGGINH+NAAAVM IG+PNL+GVAVVSALFDRQCVLE A KLHATLVEATTSNV
Sbjct: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEEALKLHATLVEATTSNV 535

[Close]

BLAST of MELO3C022605.jh1 vs. NCBI nr
Match: XP_004140412.1 (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 989 bits (2556), Expect = 0.0
Identity = 507/532 (95.30%), Postives = 519/532 (97.56%), Query Frame = 0


Query: 1   MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA 60
           MVPLPLISQIPKFNQVSRFCMAMKKQ+EMVVASS+R ET IPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVPLPLISQIPKFNQVSRFCMAMKKQDEMVVASSNRNETCIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITAITAQNTVGVQDVN+VPEGFVSKQLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNVVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180
           STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGP IISVLQE+LLPMADLVTPNLKE
Sbjct: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPAIISVLQEKLLPMADLVTPNLKE 180

Query: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLG MPL TISDMRHAA LI+QMGSKNVL+KGGDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGDMPLTTISDMRHAAMLIYQMGSKNVLIKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300
           RIKSRNTHGTGCSLASCI+AELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD
Sbjct: 241 RIKSRNTHGTGCSLASCIAAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300

Query: 301 HLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAALEGGATIVQIRE 360
           HLC LK+RE SSYSQGCFNP DLFLYAVTDSGMNERWDRSITDAVK A+EGGATIVQIRE
Sbjct: 301 HLCCLKNREPSSYSQGCFNPADLFLYAVTDSGMNERWDRSITDAVKDAVEGGATIVQIRE 360

Query: 361 KDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG 420
           KDAKTRDFLEVAKSCIKIC AHGVPLLINDRIDIALAC+ADGVHVGQSDIPAHEVRRLLG
Sbjct: 361 KDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRLLG 420

Query: 421 PNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480
           PNK+IGVSCKT EQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV
Sbjct: 421 PNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480

Query: 481 AIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATT 532
           AIGGINHTNAAAVMGIGIPNL+GVAVVSALFDRQCVLE ASKLHATLVEATT
Sbjct: 481 AIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 532

[Close]

BLAST of MELO3C022605.jh1 vs. NCBI nr
Match: KAE8648089.1 (hypothetical protein Csa_004689 [Cucumis sativus])

HSP 1 Score: 955 bits (2469), Expect = 0.0
Identity = 495/532 (93.05%), Postives = 507/532 (95.30%), Query Frame = 0


Query: 1   MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA 60
           MVPLPLISQIPK            KQ+EMVVASS+R ET IPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVPLPLISQIPK------------KQDEMVVASSNRNETCIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITAITAQNTVGVQDVN+VPEGFVSKQLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNVVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180
           STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGP IISVLQE+LLPMADLVTPNLKE
Sbjct: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPAIISVLQEKLLPMADLVTPNLKE 180

Query: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLG MPL TISDMRHAA LI+QMGSKNVL+KGGDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGDMPLTTISDMRHAAMLIYQMGSKNVLIKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300
           RIKSRNTHGTGCSLASCI+AELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD
Sbjct: 241 RIKSRNTHGTGCSLASCIAAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300

Query: 301 HLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAALEGGATIVQIRE 360
           HLC LK+RE SSYSQGCFNP DLFLYAVTDSGMNERWDRSITDAVK A+EGGATIVQIRE
Sbjct: 301 HLCCLKNREPSSYSQGCFNPADLFLYAVTDSGMNERWDRSITDAVKDAVEGGATIVQIRE 360

Query: 361 KDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG 420
           KDAKTRDFLEVAKSCIKIC AHGVPLLINDRIDIALAC+ADGVHVGQSDIPAHEVRRLLG
Sbjct: 361 KDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRLLG 420

Query: 421 PNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480
           PNK+IGVSCKT EQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV
Sbjct: 421 PNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480

Query: 481 AIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATT 532
           AIGGINHTNAAAVMGIGIPNL+GVAVVSALFDRQCVLE ASKLHATLVEATT
Sbjct: 481 AIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 520

[Close]

BLAST of MELO3C022605.jh1 vs. ExPASy Swiss-Prot
Match: Q5M731 (Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TH1 PE=1 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 7.2e-199
Identity = 351/491 (71.49%), Postives = 415/491 (84.52%), Query Frame = 0


Query: 40  RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVS 99
           ++P VL+VAGSDSGAGAGIQADLK CAARGVYC++VITA+TAQNT GVQ V+++P  F+S
Sbjct: 29  KVPQVLTVAGSDSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFIS 88

Query: 100 KQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTI 159
           +QLKSVLSD + DVVKTGMLPST IV+VL Q L +FPVRALVVDPVMVSTSG VLAG +I
Sbjct: 89  EQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSI 148

Query: 160 ISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLP 219
           +S+ +E LLP+AD++TPN+KEASALL G  ++T+++MR AA  +H+MG + VLVKGGDLP
Sbjct: 149 LSIFRERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLP 208

Query: 220 DSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFI 279
           DS D+VD++FDGK+ HELRS RI +RNTHGTGC+LASCI+AELAKGSSM SAVK +K+F+
Sbjct: 209 DSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFV 268

Query: 280 ERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDR 339
           + AL YSKDI IG G QGPFDH   LK   QSS     FNP DLFLYAVTDS MN++W+R
Sbjct: 269 DNALDYSKDIVIGSGMQGPFDHFFGLKKDPQSSRC-SIFNPDDLFLYAVTDSRMNKKWNR 328

Query: 340 SITDAVKAALEGGATIVQIREKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACD 399
           SI DA+KAA+EGGATI+Q+REK+A+TR+FLE AK+CI IC +HGV LLINDRIDIALACD
Sbjct: 329 SIVDALKAAIEGGATIIQLREKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACD 388

Query: 400 ADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKAN 459
           ADGVHVGQSD+P   VR LLGP+K+IGVSCKT EQA QAW DGADYIG GGV+PTNTKAN
Sbjct: 389 ADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKAN 448

Query: 460 NLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEA 519
           N T+G+DGLK VC ASKLPVVAIGGI  +NA +VM I  PNL+GVAVVSALFD+ CVL  
Sbjct: 449 NRTIGLDGLKEVCEASKLPVVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQ 508

Query: 520 ASKLHATLVEA 531
           A KLH TL E+
Sbjct: 509 AKKLHKTLKES 518

[Close]

BLAST of MELO3C022605.jh1 vs. ExPASy Swiss-Prot
Match: O48881 (Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus OX=3708 GN=BTH1 PE=1 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 2.6e-193
Identity = 340/491 (69.25%), Postives = 410/491 (83.50%), Query Frame = 0


Query: 40  RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVS 99
           ++  VL+VAGSDSGAGAGIQAD+K CAARGVYC++V TA+ A+NT  VQ V+++P   VS
Sbjct: 31  KVAQVLTVAGSDSGAGAGIQADIKVCAARGVYCASVKTAVKAKNTRAVQSVHLLPPDSVS 90

Query: 100 KQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTI 159
           +QLKSVLSD +VDVVKTGMLPS  IV+VL Q L E+PVRALVVDPVMVSTSG VLAG +I
Sbjct: 91  EQLKSVLSDFEVDVVKTGMLPSPEIVEVLLQNLSEYPVRALVVDPVMVSTSGHVLAGSSI 150

Query: 160 ISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLP 219
           +S+ +E LLP+AD++TPN+KEASALLGG+ ++T+++MR AA  +HQMG + VLVKGGDLP
Sbjct: 151 LSIFRERLLPLADIITPNVKEASALLGGVRIQTVAEMRSAAKSLHQMGPRFVLVKGGDLP 210

Query: 220 DSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFI 279
           DS D+VD++FDG + HEL S RI +RNTHGTGC+LASCI+AELAKGS+M SAVK +K+F+
Sbjct: 211 DSSDSVDVYFDGNEFHELHSPRIATRNTHGTGCTLASCIAAELAKGSNMLSAVKVAKRFV 270

Query: 280 ERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDR 339
           + AL YSKDI IG G QGPFDH   LK  +  SY Q  F P DLFLYAVTDS MN++W+R
Sbjct: 271 DSALNYSKDIVIGSGMQGPFDHFLSLK--DPQSYRQSTFKPDDLFLYAVTDSRMNKKWNR 330

Query: 340 SITDAVKAALEGGATIVQIREKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACD 399
           SI DAVKAA+EGGATI+Q+REK+A+TR+FLE AKSC+ IC ++GV LLINDR DIA+A D
Sbjct: 331 SIVDAVKAAIEGGATIIQLREKEAETREFLEEAKSCVDICRSNGVCLLINDRFDIAIALD 390

Query: 400 ADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKAN 459
           ADGVHVGQSD+P   VR LLGP+K+IGVSCKT EQA QAW DGADYIG GGV+PTNTKAN
Sbjct: 391 ADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTQEQAHQAWKDGADYIGSGGVFPTNTKAN 450

Query: 460 NLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEA 519
           N T+G+DGL+ VC ASKLPVVAIGGI  +NA +VM IG PNL+GVAVVSALFD++CVL  
Sbjct: 451 NRTIGLDGLREVCKASKLPVVAIGGIGISNAESVMRIGEPNLKGVAVVSALFDQECVLTQ 510

Query: 520 ASKLHATLVEA 531
           A KLH TL E+
Sbjct: 511 AKKLHKTLTES 519

[Close]

BLAST of MELO3C022605.jh1 vs. ExPASy Swiss-Prot
Match: Q2QWK9 (Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0192500 PE=2 SV=2)

HSP 1 Score: 668.7 bits (1724), Expect = 5.5e-191
Identity = 330/508 (64.96%), Postives = 414/508 (81.50%), Query Frame = 0


Query: 24  KKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQN 83
           ++   +  ++S   E   PHVL+VAGSDSG GAGIQAD+K CAA G YCS+V+TA+TAQN
Sbjct: 39  RRYNRLAASASAAREMPWPHVLTVAGSDSGGGAGIQADIKACAALGAYCSSVVTAVTAQN 98

Query: 84  TVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRALVVD 143
           T GVQ +++VPE F+ +QL SVLSDM VDVVKTGMLPS G+V+VL + LK+FPV+ALVVD
Sbjct: 99  TAGVQGIHVVPEEFIREQLNSVLSDMSVDVVKTGMLPSIGVVRVLCESLKKFPVKALVVD 158

Query: 144 PVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLI 203
           PVMVSTSGD L+  + +SV ++EL  MAD+VTPN+KEAS LLGG+ L+T+SDMR+AA  I
Sbjct: 159 PVMVSTSGDTLSESSTLSVYRDELFAMADIVTPNVKEASRLLGGVSLRTVSDMRNAAESI 218

Query: 204 HQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISAELA 263
           ++ G K+VLVKGGD+ +S DA D+FFDGK+  EL + RIK+ NTHGTGC+LASCI++ELA
Sbjct: 219 YKFGPKHVLVKGGDMLESSDATDVFFDGKEFIELHAHRIKTHNTHGTGCTLASCIASELA 278

Query: 264 KGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNPTDL 323
           KG++M  AV+ +K F+E AL +SKD+ +G+GPQGPFDHL +LK    +  SQ  F P  L
Sbjct: 279 KGATMLHAVQVAKNFVESALHHSKDLVVGNGPQGPFDHLFKLKCPPYNVGSQPSFKPDQL 338

Query: 324 FLYAVTDSGMNERWDRSITDAVKAALEGGATIVQIREKDAKTRDFLEVAKSCIKICHAHG 383
           FLYAVTDSGMN++W RSI +AV+AA+EGGATIVQ+REKD++TR+FLE AK+C++IC + G
Sbjct: 339 FLYAVTDSGMNKKWGRSIKEAVQAAIEGGATIVQLREKDSETREFLEAAKACMEICKSSG 398

Query: 384 VPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWIDGA 443
           VPLLINDR+DIALAC+ADGVHVGQ D+ AHEVR LLGP K+IGVSCKT  QA+QAW DGA
Sbjct: 399 VPLLINDRVDIALACNADGVHVGQLDMSAHEVRELLGPGKIIGVSCKTPAQAQQAWNDGA 458

Query: 444 DYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPNLRG 503
           DYIGCGGV+PT+TKANN T+G DGLK VCLASKLPVVAIGGIN +NA +VM +G+PNL+G
Sbjct: 459 DYIGCGGVFPTSTKANNPTLGFDGLKTVCLASKLPVVAIGGINASNAGSVMELGLPNLKG 518

Query: 504 VAVVSALFDRQCVLEAASKLHATLVEAT 532
           VAVVSALFDR  V+     + + L   +
Sbjct: 519 VAVVSALFDRPSVVAETRNMKSILTNTS 546

[Close]

BLAST of MELO3C022605.jh1 vs. ExPASy Swiss-Prot
Match: P61422 (Thiamine biosynthesis bifunctional protein ThiED OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) OX=243231 GN=thiDE PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 3.4e-55
Identity = 129/255 (50.59%), Postives = 167/255 (65.49%), Query Frame = 0


Query: 44  VLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLK 103
           VL+VAGSDSG GAGIQADLKT    G Y S+V+TA+TAQNT GV  ++ VP  FV+ QL 
Sbjct: 228 VLTVAGSDSGGGAGIQADLKTVTLLGSYGSSVLTALTAQNTRGVSGIHGVPPAFVADQLD 287

Query: 104 SVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVL 163
           +V SD+ VDVVKTGML S   +  +   L E+  R +VVDPVMV+  G  L     +SVL
Sbjct: 288 AVFSDIPVDVVKTGMLFSAETIVAIAAKLTEYRRRMVVVDPVMVAKGGANLIDRGAVSVL 347

Query: 164 QEELLPMADLVTPNLKEASALLGGMPLKTISD---MRHAATLIHQMGSKNVLVKGGDLPD 223
           +E L P+A LVTPN+ EA  L G      ISD   MR AA  +H++G++NVL+KGG L  
Sbjct: 348 KERLFPLAYLVTPNIPEAERLTGA----NISDEESMREAARRLHRLGARNVLLKGGHLLA 407

Query: 224 SLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIE 283
             D+VDI FDG   H   S RI S+NTHGTGC+ AS I+  LA+G  +  A+  +K++I 
Sbjct: 408 G-DSVDILFDGAAFHRFVSPRILSKNTHGTGCTFASAIATYLAQGDPLREAIARAKRYIT 467

Query: 284 RALRYSKDINIGHGP 296
            A+R ++ +  GHGP
Sbjct: 468 AAIRLAQPLGRGHGP 477

[Close]

BLAST of MELO3C022605.jh1 vs. ExPASy Swiss-Prot
Match: P56904 (Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase OS=Rhizobium meliloti (strain 1021) OX=266834 GN=thiD PE=3 SV=2)

HSP 1 Score: 205.7 bits (522), Expect = 1.3e-51
Identity = 126/260 (48.46%), Postives = 165/260 (63.46%), Query Frame = 0


Query: 45  LSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKS 104
           LS+AGSDSG GAGIQADLKT +A GVY ++VITAITAQNT GV  V  V    VS Q+ +
Sbjct: 6   LSIAGSDSGGGAGIQADLKTFSALGVYGASVITAITAQNTRGVTAVEDVSAEIVSAQMDA 65

Query: 105 VLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQ 164
           V SD+ V  VK GM+     +  +   L+ F  RA VVDPVMV+TSGD L  P  ++ L 
Sbjct: 66  VFSDLDVKAVKIGMVSRRETIAAIADGLRRFGKRA-VVDPVMVATSGDALLRPDAVAALI 125

Query: 165 EELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDA 224
           EELLP+A +VTPNL EA+ + G       ++M   A  I + G+  VLVKGG L    +A
Sbjct: 126 EELLPLALVVTPNLAEAALMTGRAIAGDEAEMARQAEAIMRTGAHAVLVKGGHLKGQ-EA 185

Query: 225 VDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALR 284
            D+FFDG  L  L + RI++RN HGTGC+L++ I+A LAKG  +  AV A+K ++  A+ 
Sbjct: 186 TDLFFDGDTLVRLPAGRIETRNDHGTGCTLSAAIAAGLAKGVPLIEAVSAAKAYLHAAIS 245

Query: 285 YSKDINIGHGPQGPFDHLCR 305
            +  + IG G +GP  H  R
Sbjct: 246 AADRLEIGQG-RGPVHHFHR 262

[Close]

BLAST of MELO3C022605.jh1 vs. ExPASy TrEMBL
Match: A0A1S3CCI6 (Thiamine-phosphate pyrophosphorylase OS=Cucumis melo OX=3656 GN=LOC103499122 PE=3 SV=1)

HSP 1 Score: 1040 bits (2689), Expect = 0.0
Identity = 534/535 (99.81%), Postives = 535/535 (100.00%), Query Frame = 0


Query: 1   MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA 60
           MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180
           STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE
Sbjct: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180

Query: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300
           RIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD
Sbjct: 241 RIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300

Query: 301 HLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAALEGGATIVQIRE 360
           HLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAA+EGGATIVQIRE
Sbjct: 301 HLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAAVEGGATIVQIRE 360

Query: 361 KDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG 420
           KDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG
Sbjct: 361 KDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG 420

Query: 421 PNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480
           PNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV
Sbjct: 421 PNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480

Query: 481 AIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV 535
           AIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV
Sbjct: 481 AIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV 535

[Close]

BLAST of MELO3C022605.jh1 vs. ExPASy TrEMBL
Match: A0A1S3CDB7 (Thiamine-phosphate pyrophosphorylase OS=Cucumis melo OX=3656 GN=LOC103499122 PE=3 SV=1)

HSP 1 Score: 1000 bits (2585), Expect = 0.0
Identity = 514/515 (99.81%), Postives = 515/515 (100.00%), Query Frame = 0


Query: 21  MAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAIT 80
           MAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAIT
Sbjct: 1   MAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAIT 60

Query: 81  AQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRAL 140
           AQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRAL
Sbjct: 61  AQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRAL 120

Query: 141 VVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAA 200
           VVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAA
Sbjct: 121 VVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAA 180

Query: 201 TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISA 260
           TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISA
Sbjct: 181 TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISA 240

Query: 261 ELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNP 320
           ELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNP
Sbjct: 241 ELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNP 300

Query: 321 TDLFLYAVTDSGMNERWDRSITDAVKAALEGGATIVQIREKDAKTRDFLEVAKSCIKICH 380
           TDLFLYAVTDSGMNERWDRSITDAVKAA+EGGATIVQIREKDAKTRDFLEVAKSCIKICH
Sbjct: 301 TDLFLYAVTDSGMNERWDRSITDAVKAAVEGGATIVQIREKDAKTRDFLEVAKSCIKICH 360

Query: 381 AHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWI 440
           AHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWI
Sbjct: 361 AHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWI 420

Query: 441 DGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPN 500
           DGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPN
Sbjct: 421 DGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPN 480

Query: 501 LRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV 535
           LRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV
Sbjct: 481 LRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV 515

[Close]

BLAST of MELO3C022605.jh1 vs. ExPASy TrEMBL
Match: A0A6J1GYV1 (Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC111458768 PE=3 SV=1)

HSP 1 Score: 940 bits (2429), Expect = 0.0
Identity = 478/531 (90.02%), Postives = 504/531 (94.92%), Query Frame = 0


Query: 1   MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA 60
           MV LPL  QIPKFNQVSRFCMAMKK EE VVASSDR+E RIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRHEMRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITA+TAQNTVGVQDVNI+PEGFVS+QLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIIPEGFVSEQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180
           STGI+QV+HQ LKEFPV+ALVVDPVMVSTSGDVLA PTIISVLQ+ELLPMADLVTPNLKE
Sbjct: 121 STGIIQVIHQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 180

Query: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGGMPL TISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300
           RI +RNTHGTGCSLASCI+AELAKGSSMFSAVK SKQFIERAL YSKDI+IG+GPQGPFD
Sbjct: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFD 300

Query: 301 HLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAALEGGATIVQIRE 360
           HLCRLKSREQSSY QG FN +DLFLYAVTDSGMN+RWDRSITDAVKAA+EGGATI+QIRE
Sbjct: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360

Query: 361 KDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG 420
           KDAKTRDFLE AKSCI+ICH HGVPLLINDRID+ALAC ADGVH+GQSDIP H  R LLG
Sbjct: 361 KDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420

Query: 421 PNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480
           P+K+IGVSCKT EQAEQAW+DGADYIGCGGVYPTNTKANNLTVG+DGLKRVCLASKLPVV
Sbjct: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVV 480

Query: 481 AIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEAT 531
           AIGGINH+NAAAVM IG+PNL+GVAVVSALFDRQCVLE   KLHATLVEAT
Sbjct: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEAT 531

[Close]

BLAST of MELO3C022605.jh1 vs. ExPASy TrEMBL
Match: A0A6J1KCE1 (Thiamine-phosphate pyrophosphorylase OS=Cucurbita maxima OX=3661 GN=LOC111492599 PE=3 SV=1)

HSP 1 Score: 934 bits (2415), Expect = 0.0
Identity = 476/531 (89.64%), Postives = 502/531 (94.54%), Query Frame = 0


Query: 1   MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA 60
           MV LPL  QIPKFNQVSRFCMAMKK EE VVASSDRYE RIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRYEMRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITA+TAQNTVGVQDVNI+PEGFVS+QLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIMPEGFVSEQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180
           STGI+QV+ Q LKEFPV+ALVVDPVMVSTSGDVLA PTIISVLQ+ELLPM DLVTPNLKE
Sbjct: 121 STGIIQVICQRLKEFPVQALVVDPVMVSTSGDVLACPTIISVLQDELLPMVDLVTPNLKE 180

Query: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGGMPL TISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300
           RI +RNTHGTGCSLASCI+AELAKGSSMFSAVK SKQFIERAL YSKDI+IG+GPQGPFD
Sbjct: 241 RITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALNYSKDISIGNGPQGPFD 300

Query: 301 HLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAALEGGATIVQIRE 360
           HLCRLKSREQSSY QG FN +DLFLYAVTDSGMN+RWDRSITDAVKAA+EGGATI+QIRE
Sbjct: 301 HLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIRE 360

Query: 361 KDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG 420
           KDAKTRDFLE AKSCI+ICH HGVPLLINDRID+ALAC ADGVH+GQSDIP H  R LLG
Sbjct: 361 KDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLG 420

Query: 421 PNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480
           P+K+IGVSCKT EQAEQAW+DGADYIGCGGVYPTNTKANNLTVG+DGLK+VCLASKLPVV
Sbjct: 421 PDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKKVCLASKLPVV 480

Query: 481 AIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEAT 531
           AIGGINH+NAAAVM IG+PNL+GVAVVSALFDRQCVLE   KLHATLVEAT
Sbjct: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEAT 531

[Close]

BLAST of MELO3C022605.jh1 vs. ExPASy TrEMBL
Match: A0A6J1GZX4 (Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC111458768 PE=3 SV=1)

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 461/511 (90.22%), Postives = 487/511 (95.30%), Query Frame = 0


Query: 21  MAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAIT 80
           MAMKK EE VVASSDR+E RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITA+T
Sbjct: 1   MAMKKPEETVVASSDRHEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVT 60

Query: 81  AQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRAL 140
           AQNTVGVQDVNI+PEGFVS+QLKSVLSDMQVDVVKTGMLPSTGI+QV+HQ LKEFPV+AL
Sbjct: 61  AQNTVGVQDVNIIPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQVIHQRLKEFPVQAL 120

Query: 141 VVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAA 200
           VVDPVMVSTSGDVLA PTIISVLQ+ELLPMADLVTPNLKEASALLGGMPL TISDMRHAA
Sbjct: 121 VVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKEASALLGGMPLNTISDMRHAA 180

Query: 201 TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISA 260
           TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRI +RNTHGTGCSLASCI+A
Sbjct: 181 TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAA 240

Query: 261 ELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNP 320
           ELAKGSSMFSAVK SKQFIERAL YSKDI+IG+GPQGPFDHLCRLKSREQSSY QG FN 
Sbjct: 241 ELAKGSSMFSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQ 300

Query: 321 TDLFLYAVTDSGMNERWDRSITDAVKAALEGGATIVQIREKDAKTRDFLEVAKSCIKICH 380
           +DLFLYAVTDSGMN+RWDRSITDAVKAA+EGGATI+QIREKDAKTRDFLE AKSCI+ICH
Sbjct: 301 SDLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICH 360

Query: 381 AHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWI 440
            HGVPLLINDRID+ALAC ADGVH+GQSDIP H  R LLGP+K+IGVSCKT EQAEQAW+
Sbjct: 361 THGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWL 420

Query: 441 DGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPN 500
           DGADYIGCGGVYPTNTKANNLTVG+DGLKRVCLASKLPVVAIGGINH+NAAAVM IG+PN
Sbjct: 421 DGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPN 480

Query: 501 LRGVAVVSALFDRQCVLEAASKLHATLVEAT 531
           L+GVAVVSALFDRQCVLE   KLHATLVEAT
Sbjct: 481 LKGVAVVSALFDRQCVLEETLKLHATLVEAT 511

[Close]

BLAST of MELO3C022605.jh1 vs. TAIR 10
Match: AT1G22940.1 (thiamin biosynthesis protein, putative )

HSP 1 Score: 694.9 bits (1792), Expect = 5.1e-200
Identity = 351/491 (71.49%), Postives = 415/491 (84.52%), Query Frame = 0


Query: 40  RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVS 99
           ++P VL+VAGSDSGAGAGIQADLK CAARGVYC++VITA+TAQNT GVQ V+++P  F+S
Sbjct: 29  KVPQVLTVAGSDSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFIS 88

Query: 100 KQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTI 159
           +QLKSVLSD + DVVKTGMLPST IV+VL Q L +FPVRALVVDPVMVSTSG VLAG +I
Sbjct: 89  EQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSI 148

Query: 160 ISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLP 219
           +S+ +E LLP+AD++TPN+KEASALL G  ++T+++MR AA  +H+MG + VLVKGGDLP
Sbjct: 149 LSIFRERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLP 208

Query: 220 DSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFI 279
           DS D+VD++FDGK+ HELRS RI +RNTHGTGC+LASCI+AELAKGSSM SAVK +K+F+
Sbjct: 209 DSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFV 268

Query: 280 ERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDR 339
           + AL YSKDI IG G QGPFDH   LK   QSS     FNP DLFLYAVTDS MN++W+R
Sbjct: 269 DNALDYSKDIVIGSGMQGPFDHFFGLKKDPQSSRC-SIFNPDDLFLYAVTDSRMNKKWNR 328

Query: 340 SITDAVKAALEGGATIVQIREKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACD 399
           SI DA+KAA+EGGATI+Q+REK+A+TR+FLE AK+CI IC +HGV LLINDRIDIALACD
Sbjct: 329 SIVDALKAAIEGGATIIQLREKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACD 388

Query: 400 ADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKAN 459
           ADGVHVGQSD+P   VR LLGP+K+IGVSCKT EQA QAW DGADYIG GGV+PTNTKAN
Sbjct: 389 ADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKAN 448

Query: 460 NLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEA 519
           N T+G+DGLK VC ASKLPVVAIGGI  +NA +VM I  PNL+GVAVVSALFD+ CVL  
Sbjct: 449 NRTIGLDGLKEVCEASKLPVVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQ 508

Query: 520 ASKLHATLVEA 531
           A KLH TL E+
Sbjct: 509 AKKLHKTLKES 518

[Close]

BLAST of MELO3C022605.jh1 vs. TAIR 10
Match: AT5G37850.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 44.3 bits (103), Expect = 3.6e-04
Identity = 30/104 (28.85%), Postives = 53/104 (50.96%), Query Frame = 0


Query: 114 VKTGMLPSTG----IVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLP 173
           V TG + S      I++V+++     P    V DPVM    G +     ++ V +E+++P
Sbjct: 125 VLTGYIGSVSFLDTILEVINKLRSVNPNLTYVCDPVM-GDEGKLYVPEELVHVYREKVVP 184

Query: 174 MADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLV 214
           +A ++TPN  EA  L  G+ + +  D R A  ++H  G   V++
Sbjct: 185 LASMLTPNQFEAEKLT-GLRINSEEDGREACAILHAAGPSKVVI 226

[Close]

BLAST of MELO3C022605.jh1 vs. TAIR 10
Match: AT5G37850.2 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 44.3 bits (103), Expect = 3.6e-04
Identity = 30/104 (28.85%), Postives = 53/104 (50.96%), Query Frame = 0


Query: 114 VKTGMLPSTG----IVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLP 173
           V TG + S      I++V+++     P    V DPVM    G +     ++ V +E+++P
Sbjct: 91  VLTGYIGSVSFLDTILEVINKLRSVNPNLTYVCDPVM-GDEGKLYVPEELVHVYREKVVP 150

Query: 174 MADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLV 214
           +A ++TPN  EA  L  G+ + +  D R A  ++H  G   V++
Sbjct: 151 LASMLTPNQFEAEKLT-GLRINSEEDGREACAILHAAGPSKVVI 192

The following BLAST results are available for this feature:

BLAST of MELO3C022605.jh1 vs. NCBI nr


Match Name	Identity	Description
XP_008460243.1	99.81	PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ...	[more]
XP_008460244.1	99.81	PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ...	[more]
XP_038876926.1	94.02	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Benincasa hispida]	[more]
XP_004140412.1	95.30	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucumis...	[more]
KAE8648089.1	93.05	hypothetical protein Csa_004689 [Cucumis sativus]	[more]

BLAST of MELO3C022605.jh1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5M731	7.2e-199	71.49	Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thal...	[more]
O48881	2.6e-193	69.25	Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus ...	[more]
Q2QWK9	5.5e-191	64.96	Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativ...	[more]
P61422	3.4e-55	50.59	Thiamine biosynthesis bifunctional protein ThiED OS=Geobacter sulfurreducens (st...	[more]
P56904	1.3e-51	48.46	Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase OS=Rhizobium meliloti (st...	[more]

BLAST of MELO3C022605.jh1 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A1S3CCI6	99.81	Thiamine-phosphate pyrophosphorylase OS=Cucumis melo OX=3656 GN=LOC103499122 PE=...	[more]
A0A1S3CDB7	99.81	Thiamine-phosphate pyrophosphorylase OS=Cucumis melo OX=3656 GN=LOC103499122 PE=...	[more]
A0A6J1GYV1	90.02	Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC1114587...	[more]
A0A6J1KCE1	89.64	Thiamine-phosphate pyrophosphorylase OS=Cucurbita maxima OX=3661 GN=LOC111492599...	[more]
A0A6J1GZX4	90.22	Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC1114587...	[more]

BLAST of MELO3C022605.jh1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G22940.1	5.1e-200	71.49	thiamin biosynthesis protein, putative	[more]
AT5G37850.1	3.6e-04	28.85	pfkB-like carbohydrate kinase family protein	[more]
AT5G37850.2	3.6e-04	28.85	pfkB-like carbohydrate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013749	Pyridoxamine kinase/Phosphomethylpyrimidine kinase	PFAM	PF08543	Phos_pyr_kin	coord: 37..281 e-value: 1.9E-88 score: 296.0
IPR034291	Thiamine phosphate synthase	TIGRFAM	TIGR00693	TIGR00693	coord: 311..508 e-value: 6.3E-63 score: 209.6
IPR034291	Thiamine phosphate synthase	HAMAP	MF_00097	TMP_synthase	coord: 308..514 score: 27.021515
IPR004399	Hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase domain	TIGRFAM	TIGR00097	TIGR00097	coord: 30..287 e-value: 5.1E-98 score: 325.4
IPR004399	Hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase domain	CDD	cd01169	HMPP_kinase	coord: 29..272 e-value: 2.61239E-115 score: 338.709
IPR022998	Thiamine phosphate synthase/TenI	PFAM	PF02581	TMP-TENI	coord: 311..496 e-value: 1.5E-63 score: 213.4
IPR022998	Thiamine phosphate synthase/TenI	CDD	cd00564	TMP_TenI	coord: 311..511 e-value: 1.49499E-83 score: 255.521
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 305..517 e-value: 1.7E-72 score: 244.8
IPR029056	Ribokinase-like	GENE3D	3.40.1190.20		coord: 9..304 e-value: 3.6E-99 score: 333.7
IPR029056	Ribokinase-like	SUPERFAMILY	53613	Ribokinase-like	coord: 27..284
IPR045029	Hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	PANTHER	PTHR20858	PHOSPHOMETHYLPYRIMIDINE KINASE	coord: 24..512
IPR036206	Thiamin phosphate synthase superfamily	SUPERFAMILY	51391	Thiamin phosphate synthase	coord: 307..510

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C022605.jh1.t3	MELO3C022605.jh1.t3	mRNA
MELO3C022605.jh1.t1	MELO3C022605.jh1.t1	mRNA
MELO3C022605.jh1.t2	MELO3C022605.jh1.t2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009228	thiamine biosynthetic process
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0008972	phosphomethylpyrimidine kinase activity
molecular_function	GO:0004789	thiamine-phosphate diphosphorylase activity