MELO3C021800 (gene) Melon (DHL92) v4

Overview


Name	MELO3C021800
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	Peptidase A1 domain-containing protein
Location	chr12: 17377094 .. 17378674 (+)
RNA-Seq Expression	MELO3C021800
Synteny	MELO3C021800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTTCAATATCATCAACCTCCATTGCAACAAAATTCTTGAGCCTATTCCTTCTTCTTGTACATGCCTCAAAACAAACCCTAGCTACAAACCCTAAAACCAATTTCCCCAAAGATTCTCTAGTTCTTGGTCTTGTTCATTCAAGAACATCTCTCCTTACACCCAAAAAAGGCTATAATTTCATTTCAAAGAAGAGGATGAAGGCAATGGATCAAATGGATGGTGATGATAATGTGATAGAGCCATTGAGAGAAATTAGGGATGGTTATTTAATGTCCTTATCAATAGGGACACCCCCACAAGTTGTTCAAGTGTATATGGACACTGGAAGTGATCTCACATGGGTTCCTTGTGGTAACCTCTCTTTTGATTGTCAAGATTGTGAAGAGTACCAGAACAATATTTCTGGCCCAAAATTGGCTGCCTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGACACTTGTGGTAGTTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCTTGGCTACCCTTGTGAAGGGCACTTGCCCTAGACCTTGCCCTTCTTTTGCTTATACTTATGGAGCAAGTGGGGTTGTAACTGGAAGTTTAACGAGAGATGTTCTTTTTATGCATGGAAATTATCATAATAATAATAATAATAATAGTAATAATAATAAGCAAGTCCCTAGATTTTGTTTTGGATGTGTTGGTGCAACTTACAGAGAGCCAATCGGGATTGCTGGTTTTGGTAGAGGCTTACTTTCTCTTCCTTTTCAACTAGGGTTTTCTCAGAAGGGCTTTTCTCATTGCTTTTTGCCTTTTAAATTTTCAAATAACCCTAATTTCTCAAGCCCTTTGATTCTTGGTCATCTTGCTATTTCTTCAAAAGATGAAAATTTGCAATTTACCCCTTTGTTGAAAAGTCCAATTTACCCAAACTATTACTATATTGGGCTTGAGTCAATTACCATTGGGAATGGGAATAATAATTTTAGATTTGGGGTTTCTTTTAAATTAAGAGAGATTGATACAAAGGGTAATGGAGGAATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCTAATCTTGAATCAGTGATAAGTTACCCAAGAGCAAAACAAGTCGAACTCAATACTGGGTTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTCTTCTTTTGTTGATGACTCTCAACTCCCTTCTATAACATTTCATTTTTTGAATAATGTTAGTGTTGTTTTGCCTCAAGGGAATAACTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGTTTGTTGTATCAAAGCATGGACGGTGTTGGTGACGATAACGACAGTGATGATAACGGGCCGGCTGGCATTTTCGGTAGCTTTCAACAGCAAAATCTACAGGTTGTTTATGATTTGGAGAAGGAAAGATTAGGGTTTCAAGCAATGGATTGTGTTTCTGTTGCTGCCAATCAGGGACTTCACAAGAATGTTAGAAGGAATATGATGAAAGTTGAAGTTTGA

mRNA sequence

ATGCCTTCAATATCATCAACCTCCATTGCAACAAAATTCTTGAGCCTATTCCTTCTTCTTGTACATGCCTCAAAACAAACCCTAGCTACAAACCCTAAAACCAATTTCCCCAAAGATTCTCTAGTTCTTGGTCTTGTTCATTCAAGAACATCTCTCCTTACACCCAAAAAAGGCTATAATTTCATTTCAAAGAAGAGGATGAAGGCAATGGATCAAATGGATGGTGATGATAATGTGATAGAGCCATTGAGAGAAATTAGGGATGGTTATTTAATGTCCTTATCAATAGGGACACCCCCACAAGTTGTTCAAGTGTATATGGACACTGGAAGTGATCTCACATGGGTTCCTTGTGGTAACCTCTCTTTTGATTGTCAAGATTGTGAAGAGTACCAGAACAATATTTCTGGCCCAAAATTGGCTGCCTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGACACTTGTGGTAGTTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCTTGGCTACCCTTGTGAAGGGCACTTGCCCTAGACCTTGCCCTTCTTTTGCTTATACTTATGGAGCAAGTGGGGTTGTAACTGGAAGTTTAACGAGAGATGTTCTTTTTATGCATGGAAATTATCATAATAATAATAATAATAATAGTAATAATAATAAGCAAGTCCCTAGATTTTGTTTTGGATGTGTTGGTGCAACTTACAGAGAGCCAATCGGGATTGCTGGTTTTGGTAGAGGCTTACTTTCTCTTCCTTTTCAACTAGGGTTTTCTCAGAAGGGCTTTTCTCATTGCTTTTTGCCTTTTAAATTTTCAAATAACCCTAATTTCTCAAGCCCTTTGATTCTTGGTCATCTTGCTATTTCTTCAAAAGATGAAAATTTGCAATTTACCCCTTTGTTGAAAAGTCCAATTTACCCAAACTATTACTATATTGGGCTTGAGTCAATTACCATTGGGAATGGGAATAATAATTTTAGATTTGGGGTTTCTTTTAAATTAAGAGAGATTGATACAAAGGGTAATGGAGGAATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCTAATCTTGAATCAGTGATAAGTTACCCAAGAGCAAAACAAGTCGAACTCAATACTGGGTTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTCTTCTTTTGTTGATGACTCTCAACTCCCTTCTATAACATTTCATTTTTTGAATAATGTTAGTGTTGTTTTGCCTCAAGGGAATAACTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGTTTGTTGTATCAAAGCATGGACGGTGTTGGTGACGATAACGACAGTGATGATAACGGGCCGGCTGGCATTTTCGGTAGCTTTCAACAGCAAAATCTACAGGTTGTTTATGATTTGGAGAAGGAAAGATTAGGGTTTCAAGCAATGGATTGTGTTTCTGTTGCTGCCAATCAGGGACTTCACAAGAATGTTAGAAGGAATATGATGAAAGTTGAAGTTTGA

Coding sequence (CDS)

ATGCCTTCAATATCATCAACCTCCATTGCAACAAAATTCTTGAGCCTATTCCTTCTTCTTGTACATGCCTCAAAACAAACCCTAGCTACAAACCCTAAAACCAATTTCCCCAAAGATTCTCTAGTTCTTGGTCTTGTTCATTCAAGAACATCTCTCCTTACACCCAAAAAAGGCTATAATTTCATTTCAAAGAAGAGGATGAAGGCAATGGATCAAATGGATGGTGATGATAATGTGATAGAGCCATTGAGAGAAATTAGGGATGGTTATTTAATGTCCTTATCAATAGGGACACCCCCACAAGTTGTTCAAGTGTATATGGACACTGGAAGTGATCTCACATGGGTTCCTTGTGGTAACCTCTCTTTTGATTGTCAAGATTGTGAAGAGTACCAGAACAATATTTCTGGCCCAAAATTGGCTGCCTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGACACTTGTGGTAGTTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCTTGGCTACCCTTGTGAAGGGCACTTGCCCTAGACCTTGCCCTTCTTTTGCTTATACTTATGGAGCAAGTGGGGTTGTAACTGGAAGTTTAACGAGAGATGTTCTTTTTATGCATGGAAATTATCATAATAATAATAATAATAATAGTAATAATAATAAGCAAGTCCCTAGATTTTGTTTTGGATGTGTTGGTGCAACTTACAGAGAGCCAATCGGGATTGCTGGTTTTGGTAGAGGCTTACTTTCTCTTCCTTTTCAACTAGGGTTTTCTCAGAAGGGCTTTTCTCATTGCTTTTTGCCTTTTAAATTTTCAAATAACCCTAATTTCTCAAGCCCTTTGATTCTTGGTCATCTTGCTATTTCTTCAAAAGATGAAAATTTGCAATTTACCCCTTTGTTGAAAAGTCCAATTTACCCAAACTATTACTATATTGGGCTTGAGTCAATTACCATTGGGAATGGGAATAATAATTTTAGATTTGGGGTTTCTTTTAAATTAAGAGAGATTGATACAAAGGGTAATGGAGGAATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCTAATCTTGAATCAGTGATAAGTTACCCAAGAGCAAAACAAGTCGAACTCAATACTGGGTTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTCTTCTTTTGTTGATGACTCTCAACTCCCTTCTATAACATTTCATTTTTTGAATAATGTTAGTGTTGTTTTGCCTCAAGGGAATAACTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGTTTGTTGTATCAAAGCATGGACGGTGTTGGTGACGATAACGACAGTGATGATAACGGGCCGGCTGGCATTTTCGGTAGCTTTCAACAGCAAAATCTACAGGTTGTTTATGATTTGGAGAAGGAAAGATTAGGGTTTCAAGCAATGGATTGTGTTTCTGTTGCTGCCAATCAGGGACTTCACAAGAATGTTAGAAGGAATATGATGAAAGTTGAAGTTTGA

Protein sequence

MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYNFISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCFGCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAISSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRNMMKVEV

Homology

[Close]

BLAST of MELO3C021800 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 6.9e-53
Identity = 150/451 (33.26%), Postives = 202/451 (44.79%), Query Frame = 0


Query: 90  YLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPKLAAFLPTHSS 149
           YL+SLS+G+    V +Y+DTGSDL W PC    F C  CE      S P       + SS
Sbjct: 83  YLISLSVGSSSSAVSLYLDTGSDLVWFPC--RPFTCILCESKPLPPSPPS------SLSS 142

Query: 150 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGTC---PRPCPSFAYTYGASGVV 209
           ++   +C S  C   HSS    D C I+ C L  +  G C     PCP F Y YG  G +
Sbjct: 143 SATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYG-DGSL 202

Query: 210 TGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCFGCVGATYREPIGIAGFGRGLLSLPFQ 269
              L  D L             S  +  V  F FGC   T  EPIG+AGFGRG LSLP Q
Sbjct: 203 VAKLYSDSL-------------SLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQ 262

Query: 270 LGFSQ----KGFSHCFLPFKF-SNNPNFSSPLILGHLA------------------ISSK 329
           L          FS+C +   F S+     SPLILG                        K
Sbjct: 263 LAVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKK 322

Query: 330 DENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGT 389
                FT +L++P +P +Y + L+ I+IG  N          LR ID  G GG+++DSGT
Sbjct: 323 KNEFVFTEMLENPKHPYFYSVSLQGISIGKRN----IPAPAMLRRIDKNGGGGVVVDSGT 382

Query: 390 TYTHLPEPLYSQLISNLESVIS--YPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPS 449
           T+T LP   Y+ ++   +S +   + RA +VE ++G   CY +             ++P+
Sbjct: 383 TFTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN---------QTVKVPA 442

Query: 450 ITFHFL-NNVSVVLPQGNNFYAMA----APINSTVVKCLLYQSMDGVGDDNDSDDNGPAG 508
           +  HF  N  SV LP+ N FY              + CL+  +    G D      G   
Sbjct: 443 LVLHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMN----GGDESELRGGTGA 494

[Close]

BLAST of MELO3C021800 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 7.2e-34
Identity = 128/446 (28.70%), Postives = 179/446 (40.13%), Query Frame = 0


Query: 64  KKRMKAMDQMDGDDNVIEPLREIRDG-YLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLS 123
           ++RM++++ M    + IE      DG YLM+++IGTP       MDTGSDL W  C    
Sbjct: 69  ERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCE--- 128

Query: 124 FDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLA 183
             C  C      I       F P  SS+     C S +C D+     P + C    C   
Sbjct: 129 -PCTQCFSQPTPI-------FNPQDSSSFSTLPCESQYCQDL-----PSETCNNNEC--- 188

Query: 184 TLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCFGC 243
                        + Y YG      G +  +               +     VP   FGC
Sbjct: 189 ------------QYTYGYGDGSTTQGYMATETF-------------TFETSSVPNIAFGC 248

Query: 244 ----VGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHL 303
                G       G+ G G G LSLP QLG  Q  FS+C   +  S+     S L LG  
Sbjct: 249 GEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQ--FSYCMTSYGSSS----PSTLALGS- 308

Query: 304 AISSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGML 363
           A S   E    T L+ S + P YYYI L+ IT+G  N     G+     ++   G GGM+
Sbjct: 309 AASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDN----LGIPSSTFQLQDDGTGGMI 368

Query: 364 IDSGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQ 423
           IDSGTT T+LP+  Y+ +       I+ P     E ++G   C++ P   +        Q
Sbjct: 369 IDSGTTLTYLPQDAYNAVAQAFTDQINLPTVD--ESSSGLSTCFQQPSDGST------VQ 428

Query: 424 LPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIF 483
           +P I+  F   V  +  Q      + +P    +  CL   S   +G            IF
Sbjct: 429 VPEISMQFDGGVLNLGEQN----ILISPAEGVI--CLAMGSSSQLG----------ISIF 435

Query: 484 GSFQQQNLQVVYDLEKERLGFQAMDC 505
           G+ QQQ  QV+YDL+   + F    C
Sbjct: 489 GNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of MELO3C021800 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.5e-31
Identity = 129/445 (28.99%), Postives = 181/445 (40.67%), Query Frame = 0


Query: 65  KRMKAMDQMDGDDNVIEPLREIRDG-YLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSF 124
           +R++ ++ M    + +E      DG YLM+LSIGTP Q     MDTGSDL W        
Sbjct: 69  RRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWT------- 128

Query: 125 DCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLAT 184
            CQ C +  N         F P  SS+     C S  C  + S                 
Sbjct: 129 QCQPCTQCFNQ----STPIFNPQGSSSFSTLPCSSQLCQALSSP---------------- 188

Query: 185 LVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCFGC- 244
               TC      + Y YG      GS+  + L             +  +  +P   FGC 
Sbjct: 189 ----TCSNNFCQYTYGYGDGSETQGSMGTETL-------------TFGSVSIPNITFGCG 248

Query: 245 ---VGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLA 304
               G       G+ G GRG LSLP QL  ++  FS+C  P   S   N    L+LG LA
Sbjct: 249 ENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTK--FSYCMTPIGSSTPSN----LLLGSLA 308

Query: 305 ISSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLI 364
            +S       T L++S   P +YYI L  +++G+         +F L      G GG++I
Sbjct: 309 -NSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPID-PSAFALN--SNNGTGGIII 368

Query: 365 DSGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQL 424
           DSGTT T+     Y  +     S I+ P       ++GFDLC++ P   +N       Q+
Sbjct: 369 DSGTTLTYFVNNAYQSVRQEFISQINLPVVN--GSSSGFDLCFQTPSDPSN------LQI 428

Query: 425 PSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFG 484
           P+   HF +   + LP  N F    +P N  +  CL   S            +    IFG
Sbjct: 429 PTFVMHF-DGGDLELPSENYF---ISPSNGLI--CLAMGS-----------SSQGMSIFG 434

Query: 485 SFQQQNLQVVYDLEKERLGFQAMDC 505
           + QQQN+ VVYD     + F +  C
Sbjct: 489 NIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of MELO3C021800 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.7e-30
Identity = 123/438 (28.08%), Postives = 183/438 (41.78%), Query Frame = 0


Query: 78  NVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISG 137
           +V+  L +    Y   L +GTP + V + +DTGSD+ W+ C      C+ C    + I  
Sbjct: 130 SVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCA----PCRRCYSQSDPI-- 189

Query: 138 PKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGTCPRPCPSFA 197
                F P  S T     C S  C  + S          AGC+     + TC      + 
Sbjct: 190 -----FDPRKSKTYATIPCSSPHCRRLDS----------AGCNTR---RKTC-----LYQ 249

Query: 198 YTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCFGC--------VGATYRE 257
            +YG      G  + + L    N             +V     GC        VGA    
Sbjct: 250 VSYGDGSFTVGDFSTETLTFRRN-------------RVKGVALGCGHDNEGLFVGAA--- 309

Query: 258 PIGIAGFGRGLLSLPFQLG--FSQKGFSHCFLPFKFSNNPNFSSPLILGHLAISSKDENL 317
             G+ G G+G LS P Q G  F+QK FS+C +    S+ P   S ++ G+ A+S      
Sbjct: 310 --GLLGLGKGKLSFPGQTGHRFNQK-FSYCLVDRSASSKP---SSVVFGNAAVS---RIA 369

Query: 318 QFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTH 377
           +FTPLL +P    +YY+GL  I++G        GV+  L ++D  GNGG++IDSGT+ T 
Sbjct: 370 RFTPLLSNPKLDTFYYVGLLGISVGGTRVP---GVTASLFKLDQIGNGGVIIDSGTSVTR 429

Query: 378 LPEPLYSQLISNLE-SVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSITFHF 437
           L  P Y  +         +  RA    L   FD C+ +       S +++ ++P++  HF
Sbjct: 430 LIRPAYIAMRDAFRVGAKTLKRAPDFSL---FDTCFDL-------SNMNEVKVPTVVLHF 484

Query: 438 LNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNL 497
                V LP  N       P+++    C  +           +   G   I G+ QQQ  
Sbjct: 490 -RGADVSLPATN----YLIPVDTNGKFCFAF-----------AGTMGGLSIIGNIQQQGF 484

Query: 498 QVVYDLEKERLGFQAMDC 505
           +VVYDL   R+GF    C
Sbjct: 550 RVVYDLASSRVGFAPGGC 484

[Close]

BLAST of MELO3C021800 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.3e-27
Identity = 121/439 (27.56%), Postives = 181/439 (41.23%), Query Frame = 0


Query: 77  DNVIEPLREIRDG---YLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQN 136
           DN  +P  ++      YLM++SIGTPP  +    DTGSDL W  C      C DC    +
Sbjct: 74  DNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA----PCDDCYTQVD 133

Query: 137 NISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGTCPRPC 196
            +  PK        SST    +C SS C  + +          A CS       TC    
Sbjct: 134 PLFDPKT-------SSTYKDVSCSSSQCTALENQ---------ASCSTN---DNTC---- 193

Query: 197 PSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCFGC----VGATYRE 256
            S++ +YG +    G++  D L +         ++     Q+     GC     G   ++
Sbjct: 194 -SYSLSYGDNSYTKGNIAVDTLTL--------GSSDTRPMQLKNIIIGCGHNNAGTFNKK 253

Query: 257 PIGIAGFGRGLLSLPFQLGFSQKG-FSHCFLPFKFSNNPNFSSPLILGHLAISSKDENLQ 316
             GI G G G +SL  QLG S  G FS+C +P   ++  + +S +  G  AI S    + 
Sbjct: 254 GSGIVGLGGGPVSLIKQLGDSIDGKFSYCLVP--LTSKKDQTSKINFGTNAIVS-GSGVV 313

Query: 317 FTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHL 376
            TPL+       +YY+ L+SI++G+    +    S           G ++IDSGTT T L
Sbjct: 314 STPLIAKASQETFYYLTLKSISVGSKQIQYSGSDS-------ESSEGNIIIDSGTTLTLL 373

Query: 377 PEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSITFHFLN 436
           P   YS+L   + S  S    K+ +  +G  LCY         S   D ++P IT HF +
Sbjct: 374 PTEFYSELEDAVAS--SIDAEKKQDPQSGLSLCY---------SATGDLKVPVITMHF-D 433

Query: 437 NVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNLQV 496
              V L   N F  +     S  + C  ++                  I+G+  Q N  V
Sbjct: 434 GADVKLDSSNAFVQV-----SEDLVCFAFRGSPSF------------SIYGNVAQMNFLV 437

Query: 497 VYDLEKERLGFQAMDCVSV 508
            YD   + + F+  DC  +
Sbjct: 494 GYDTVSKTVSFKPTDCAKM 437

[Close]

BLAST of MELO3C021800 vs. NCBI nr
Match: XP_008459091.1 (PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo] >KAA0043307.1 aspartic proteinase nepenthesin-2 [Cucumis melo var. makuwa] >TYK29371.1 aspartic proteinase nepenthesin-2 [Cucumis melo var. makuwa])

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0


Query: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240
           LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF
Sbjct: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240

Query: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300
           GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300

Query: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420
           SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRNMMKVEV 527
           FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRNMMKVEV
Sbjct: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRNMMKVEV 526

[Close]

BLAST of MELO3C021800 vs. NCBI nr
Match: XP_004145478.2 (probable aspartyl protease At4g16563 [Cucumis sativus] >KGN66888.1 hypothetical protein Csa_007266 [Cucumis sativus])

HSP 1 Score: 1017.7 bits (2630), Expect = 3.6e-293
Identity = 495/520 (95.19%), Postives = 505/520 (97.12%), Query Frame = 0


Query: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           MPSISS S ATKFLSLFLLLVH S QTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQ DGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGP+LAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240
           LA+LVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLF HGNY    NNN+NNNKQ+PRFCF
Sbjct: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNY----NNNNNNNKQIPRFCF 240

Query: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300
           GCVGATYREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 300

Query: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKDENLQFTPLLKSP+YPNYYYIGLESITIGNG+NNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420
           SGTTYTHLPEPLYSQLISNLE VI YPRAKQVELNTGFDLCYKVPCKNNNSSFVDD+QLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRN 521
           FQQQN++VVYDLEKERLGFQ MDCVSVAA QGLHKNVRRN
Sbjct: 481 FQQQNIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRN 516

[Close]

BLAST of MELO3C021800 vs. NCBI nr
Match: XP_038893627.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 930.2 bits (2403), Expect = 7.6e-267
Identity = 453/512 (88.48%), Postives = 479/512 (93.55%), Query Frame = 0


Query: 6   STSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYNFISKK 65
           STS A K LS FLLLV+ S++TLATNPKTN PKDSLV+GLVHSRT+LLTPKKGYNFIS+K
Sbjct: 5   STSFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYNFISRK 64

Query: 66  RMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDC 125
           RMKAM+    DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGNLSFDC
Sbjct: 65  RMKAMEM---DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDC 124

Query: 126 QDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV 185
           QDCEEYQNN+SGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV
Sbjct: 125 QDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV 184

Query: 186 KGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCFGCVGA 245
           K TCPRPCPSFAYTYGASGVV G+LTRDVL MH    NN N+ +++ K+ PRFCFGCVGA
Sbjct: 185 KATCPRPCPSFAYTYGASGVVIGTLTRDVLLMH---INNINSPNSSTKKTPRFCFGCVGA 244

Query: 246 TYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAISSKDE 305
           +YREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LA+SSKDE
Sbjct: 245 SYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDE 304

Query: 306 NLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTY 365
           +LQFTPLLKSPIYPNYYYIGLESITIGNGN+NFRFGVSF LREIDTKGNGGMLIDSGTTY
Sbjct: 305 HLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTY 364

Query: 366 THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSITFH 425
           THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNN SF+DDSQLPSITFH
Sbjct: 365 THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFH 424

Query: 426 FLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQN 485
           FLNNVSVVLPQ NNFYAMAAPINSTVVKCLL+QSMDGVG D D D +GPAGIFGSFQQQN
Sbjct: 425 FLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQN 484

Query: 486 LQVVYDLEKERLGFQAMDCVSVAANQGLHKNV 518
           L+VVYDLEKERLGFQ MDC  VAA QGLHKNV
Sbjct: 485 LEVVYDLEKERLGFQPMDCAYVAATQGLHKNV 510

[Close]

BLAST of MELO3C021800 vs. NCBI nr
Match: XP_022142611.1 (probable aspartyl protease At4g16563 [Momordica charantia])

HSP 1 Score: 836.6 bits (2160), Expect = 1.1e-238
Identity = 419/527 (79.51%), Postives = 460/527 (87.29%), Query Frame = 0


Query: 5   SSTSIATKFLSLFLLLV--HASKQTLATNPKTNFPK-DSLVLGLVHSRTSLLTPKKGY-- 64
           S+T+I++K L+ FLLL+   +  +T A   + NFP  DSLVLGLVHSRTSLLTPK+GY  
Sbjct: 7   SATTISSKVLTFFLLLILLLSVSETAARPHRNNFPNTDSLVLGLVHSRTSLLTPKRGYFS 66

Query: 65  -NFISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPC 124
               S    K M+++ G DNVIEPLREIRDGYL+SL++GTPPQV+QVYMDTGSDLTWVPC
Sbjct: 67  RKGSSSSINKPMEEI-GSDNVIEPLREIRDGYLISLTLGTPPQVIQVYMDTGSDLTWVPC 126

Query: 125 GNLSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAG 184
           GNLSFDCQDCEEYQNN+SGPKLA F PTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAG
Sbjct: 127 GNLSFDCQDCEEYQNNVSGPKLAPFSPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAG 186

Query: 185 CSLATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRF 244
           CSLATLVKGTCPRPCPSFAYTYGASGVVTG+LT+DV+ MHG        + N+  Q+PRF
Sbjct: 187 CSLATLVKGTCPRPCPSFAYTYGASGVVTGTLTKDVILMHG-------VSPNSTTQIPRF 246

Query: 245 CFGCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHL 304
           CFGCVGATYREPIGIAGFGRGLLSLP QLGFS KGFSHCFLPFKFSNNPNFSSPLILG L
Sbjct: 247 CFGCVGATYREPIGIAGFGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGSL 306

Query: 305 AISSKDENLQFTPLLKSPIYPNYYYIGLESITIGN--GNNNFRFGVSFKLREIDTKGNGG 364
           AISSKD +LQFTPLLKSP+YPNYYYIGLES+TIG+  GNNN RFGVS KLREIDTKGNGG
Sbjct: 307 AISSKDHSLQFTPLLKSPLYPNYYYIGLESVTIGDGIGNNNSRFGVSLKLREIDTKGNGG 366

Query: 365 MLIDSGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNN--SSFV 424
           MLIDSGTTYTHLPEPLYSQLISNLESV++YPRAKQVE+NTGFDLCYK+PCKNN   SS +
Sbjct: 367 MLIDSGTTYTHLPEPLYSQLISNLESVVTYPRAKQVEINTGFDLCYKIPCKNNTNFSSSM 426

Query: 425 DD---SQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDND--S 484
           DD   + LPSITFHFLNNVSVVLPQGNNFYAMAAP NSTVVKCLL+QSMDG G D D   
Sbjct: 427 DDYCSNLLPSITFHFLNNVSVVLPQGNNFYAMAAPTNSTVVKCLLFQSMDGGGGDGDGNG 486

Query: 485 DDNGPAGIFGSFQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKN 517
           DD+GPAGIFGSFQQQN++VVYDL+KER+GFQ MDC S AA+QGLHKN
Sbjct: 487 DDDGPAGIFGSFQQQNMEVVYDLQKERIGFQTMDCASSAASQGLHKN 525

[Close]

BLAST of MELO3C021800 vs. NCBI nr
Match: XP_023520027.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832.8 bits (2150), Expect = 1.7e-237
Identity = 413/515 (80.19%), Postives = 457/515 (88.74%), Query Frame = 0


Query: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           M SI + S     L L L+   A  QTLA NPKT F KDSLVLGLVHSRTSLLTPK+GYN
Sbjct: 1   MASIVAKSFFVLVLVLVLVSGEAMGQTLA-NPKTKFLKDSLVLGLVHSRTSLLTPKRGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
            +S+KR+K M+   G+D+VIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  SLSRKRIKPMEM--GNDDVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDC+EYQNN+ GPKLAAFLPTHSSTSIRDTCGSSFC+DIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCDEYQNNVLGPKLAAFLPTHSSTSIRDTCGSSFCIDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240
           LATLVKGTCPRPCPSF+YTYGASG+V G+LT+DV+F+HG       N+ N+++++P+FCF
Sbjct: 181 LATLVKGTCPRPCPSFSYTYGASGLVIGTLTKDVIFIHG-------NSPNSSRKIPKFCF 240

Query: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300
           GCVGATYREPIGIAGFGRGLLSLP+QLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPYQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 300

Query: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360
           SSK E+L+FTPLLKSP YPNYYYIGLESITIGNG N  RFGVS +LREIDTKGNGG+LID
Sbjct: 301 SSK-EHLKFTPLLKSPFYPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILID 360

Query: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420
           SGTTYTHLPEPLYSQLISNLES+ISYPRAK+ ELNTGFDLCYKVP K NN+ F D+ +LP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESLISYPRAKEHELNTGFDLCYKVPYK-NNTFFSDEFELP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480
           SITFHFLNNVSVVLPQGN+FYAMAAP NSTVVKCLL+QSMDG GD       GPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNSFYAMAAPSNSTVVKCLLFQSMDGDGD-------GPAGIFGS 480

Query: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHK 516
           FQQQNL+VVYDLEKERLGF+ MDC SVA +QGLHK
Sbjct: 481 FQQQNLEVVYDLEKERLGFEGMDCASVAVSQGLHK 496

[Close]

BLAST of MELO3C021800 vs. ExPASy TrEMBL
Match: A0A5A7TNC9 (Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00970 PE=3 SV=1)

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0


Query: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240
           LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF
Sbjct: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240

Query: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300
           GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300

Query: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420
           SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRNMMKVEV 527
           FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRNMMKVEV
Sbjct: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRNMMKVEV 526

[Close]

BLAST of MELO3C021800 vs. ExPASy TrEMBL
Match: A0A1S3CAK9 (aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103498305 PE=3 SV=1)

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0


Query: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240
           LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF
Sbjct: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240

Query: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300
           GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300

Query: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420
           SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRNMMKVEV 527
           FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRNMMKVEV
Sbjct: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRNMMKVEV 526

[Close]

BLAST of MELO3C021800 vs. ExPASy TrEMBL
Match: A0A0A0LYP0 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G704590 PE=3 SV=1)

HSP 1 Score: 1017.7 bits (2630), Expect = 1.8e-293
Identity = 495/520 (95.19%), Postives = 505/520 (97.12%), Query Frame = 0


Query: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           MPSISS S ATKFLSLFLLLVH S QTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQ DGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGP+LAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240
           LA+LVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLF HGNY    NNN+NNNKQ+PRFCF
Sbjct: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNY----NNNNNNNKQIPRFCF 240

Query: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300
           GCVGATYREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 300

Query: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKDENLQFTPLLKSP+YPNYYYIGLESITIGNG+NNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420
           SGTTYTHLPEPLYSQLISNLE VI YPRAKQVELNTGFDLCYKVPCKNNNSSFVDD+QLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRN 521
           FQQQN++VVYDLEKERLGFQ MDCVSVAA QGLHKNVRRN
Sbjct: 481 FQQQNIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRN 516

[Close]

BLAST of MELO3C021800 vs. ExPASy TrEMBL
Match: A0A6J1CMP8 (probable aspartyl protease At4g16563 OS=Momordica charantia OX=3673 GN=LOC111012684 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 5.5e-239
Identity = 419/527 (79.51%), Postives = 460/527 (87.29%), Query Frame = 0


Query: 5   SSTSIATKFLSLFLLLV--HASKQTLATNPKTNFPK-DSLVLGLVHSRTSLLTPKKGY-- 64
           S+T+I++K L+ FLLL+   +  +T A   + NFP  DSLVLGLVHSRTSLLTPK+GY  
Sbjct: 7   SATTISSKVLTFFLLLILLLSVSETAARPHRNNFPNTDSLVLGLVHSRTSLLTPKRGYFS 66

Query: 65  -NFISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPC 124
               S    K M+++ G DNVIEPLREIRDGYL+SL++GTPPQV+QVYMDTGSDLTWVPC
Sbjct: 67  RKGSSSSINKPMEEI-GSDNVIEPLREIRDGYLISLTLGTPPQVIQVYMDTGSDLTWVPC 126

Query: 125 GNLSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAG 184
           GNLSFDCQDCEEYQNN+SGPKLA F PTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAG
Sbjct: 127 GNLSFDCQDCEEYQNNVSGPKLAPFSPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAG 186

Query: 185 CSLATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRF 244
           CSLATLVKGTCPRPCPSFAYTYGASGVVTG+LT+DV+ MHG        + N+  Q+PRF
Sbjct: 187 CSLATLVKGTCPRPCPSFAYTYGASGVVTGTLTKDVILMHG-------VSPNSTTQIPRF 246

Query: 245 CFGCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHL 304
           CFGCVGATYREPIGIAGFGRGLLSLP QLGFS KGFSHCFLPFKFSNNPNFSSPLILG L
Sbjct: 247 CFGCVGATYREPIGIAGFGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGSL 306

Query: 305 AISSKDENLQFTPLLKSPIYPNYYYIGLESITIGN--GNNNFRFGVSFKLREIDTKGNGG 364
           AISSKD +LQFTPLLKSP+YPNYYYIGLES+TIG+  GNNN RFGVS KLREIDTKGNGG
Sbjct: 307 AISSKDHSLQFTPLLKSPLYPNYYYIGLESVTIGDGIGNNNSRFGVSLKLREIDTKGNGG 366

Query: 365 MLIDSGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNN--SSFV 424
           MLIDSGTTYTHLPEPLYSQLISNLESV++YPRAKQVE+NTGFDLCYK+PCKNN   SS +
Sbjct: 367 MLIDSGTTYTHLPEPLYSQLISNLESVVTYPRAKQVEINTGFDLCYKIPCKNNTNFSSSM 426

Query: 425 DD---SQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDND--S 484
           DD   + LPSITFHFLNNVSVVLPQGNNFYAMAAP NSTVVKCLL+QSMDG G D D   
Sbjct: 427 DDYCSNLLPSITFHFLNNVSVVLPQGNNFYAMAAPTNSTVVKCLLFQSMDGGGGDGDGNG 486

Query: 485 DDNGPAGIFGSFQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKN 517
           DD+GPAGIFGSFQQQN++VVYDL+KER+GFQ MDC S AA+QGLHKN
Sbjct: 487 DDDGPAGIFGSFQQQNMEVVYDLQKERIGFQTMDCASSAASQGLHKN 525

[Close]

BLAST of MELO3C021800 vs. ExPASy TrEMBL
Match: A0A6J1EHM1 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111434252 PE=3 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 2.0e-236
Identity = 412/515 (80.00%), Postives = 455/515 (88.35%), Query Frame = 0


Query: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           M SI++ S     L L L+   A  QTLA NPKT F KDSLVLGLVHSRTSLLTPK+GYN
Sbjct: 1   MASIAARSYFVLVLVLVLVSGEAMGQTLA-NPKTKFLKDSLVLGLVHSRTSLLTPKRGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120
            +  KR+K M+   G+D+VIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  SLLTKRIKPMEM--GNDDVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDC+EYQNN+ GPKLAAFLPTHSSTSIRDTCGSSFC+DIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCDEYQNNVLGPKLAAFLPTHSSTSIRDTCGSSFCIDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240
           LATLVKGTCPRPCPSF+YTYGASG+V G+LT+DV+F+HG       N+ N+++++P+FCF
Sbjct: 181 LATLVKGTCPRPCPSFSYTYGASGLVIGTLTKDVIFIHG-------NSPNSSRKIPKFCF 240

Query: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300
           GCVGATYREPIGIAGFGRGLLSLP QLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 300

Query: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360
           SSK E+L+FTP LKSP YPNYYYIGLESITIGNG N  RFGVS +LREIDTKGNGG+LID
Sbjct: 301 SSK-EHLKFTPFLKSPFYPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILID 360

Query: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420
           SGTTYTHLPEPLYSQLISNLES+ISYPRAK+ ELNTGFDLCYKVP K NN+ F D+ +LP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESLISYPRAKEHELNTGFDLCYKVPYK-NNTFFSDEFELP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480
           SITFHFLNNVSVVLPQGN+FYAMAAP NSTVVKCLL+QSMDG GD       GPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNSFYAMAAPSNSTVVKCLLFQSMDGDGD-------GPAGIFGS 480

Query: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHK 516
           FQQQNL+VVYDLEKERLGF+AMDC SVA +QGLHK
Sbjct: 481 FQQQNLEVVYDLEKERLGFEAMDCASVAVSQGLHK 496

[Close]

BLAST of MELO3C021800 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 597.0 bits (1538), Expect = 1.4e-170
Identity = 307/515 (59.61%), Postives = 378/515 (73.40%), Query Frame = 0


Query: 11  TKFLSLFL---LLVHASKQTLATNPKTNFPKDS--LVLGLVHSRTSLLTPKKGYNFISKK 70
           T  L LFL   LL++ + +T A   K      S  LVL L  S  SL TPK       KK
Sbjct: 5   THVLFLFLLITLLLNTTNKTQARQHKNPSSSSSSFLVLTLTKSSVSLPTPKSQTQERIKK 64

Query: 71  RMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDC 130
            + ++D       V+EPLRE+RDGYL++L+IGTPPQ VQVY+DTGSDLTWVPCGNLSFDC
Sbjct: 65  PLSSVDV------VMEPLREVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDC 124

Query: 131 QDCEEYQNN-ISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATL 190
            +C + +NN +  P  + F P HSSTS RD+C SSFC++IHSSDNPFDPC +AGCS++ L
Sbjct: 125 IECYDLKNNDLKSP--SVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSML 184

Query: 191 VKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCFGCVG 250
           +K TC RPCPSFAYTYG  G+++G LTRD+L                 + VPRF FGCV 
Sbjct: 185 LKSTCVRPCPSFAYTYGEGGLISGILTRDIL-------------KARTRDVPRFSFGCVT 244

Query: 251 ATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAIS-SK 310
           +TYREPIGIAGFGRGLLSLP QLGF +KGFSHCFLPFKF NNPN SSPLILG  A+S + 
Sbjct: 245 STYREPIGIAGFGRGLLSLPSQLGFLEKGFSHCFLPFKFVNNPNISSPLILGASALSINL 304

Query: 311 DENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGT 370
            ++LQFTP+L +P+YPN YYIGLESITI  G N     V   LR+ D++GNGGML+DSGT
Sbjct: 305 TDSLQFTPMLNTPMYPNSYYIGLESITI--GTNITPTQVPLTLRQFDSQGNGGMLVDSGT 364

Query: 371 TYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQL---P 430
           TYTHLPEP YSQL++ L+S I+YPRA + E  TGFDLCYKVPC NNN + +++  +   P
Sbjct: 365 TYTHLPEPFYSQLLTTLQSTITYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFP 424

Query: 431 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 490
           SITFHFLNN +++LPQGN+FYAM+AP + +VV+CLL+Q+M       +  D GPAG+FGS
Sbjct: 425 SITFHFLNNATLLLPQGNSFYAMSAPSDGSVVQCLLFQNM-------EDGDYGPAGVFGS 484

Query: 491 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHK 516
           FQQQN++VVYDLEKER+GFQAMDCV  AA+ GL++
Sbjct: 485 FQQQNVKVVYDLEKERIGFQAMDCVLEAASHGLNQ 489

[Close]

BLAST of MELO3C021800 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 209.9 bits (533), Expect = 4.9e-54
Identity = 150/451 (33.26%), Postives = 202/451 (44.79%), Query Frame = 0


Query: 90  YLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPKLAAFLPTHSS 149
           YL+SLS+G+    V +Y+DTGSDL W PC    F C  CE      S P       + SS
Sbjct: 83  YLISLSVGSSSSAVSLYLDTGSDLVWFPC--RPFTCILCESKPLPPSPPS------SLSS 142

Query: 150 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGTC---PRPCPSFAYTYGASGVV 209
           ++   +C S  C   HSS    D C I+ C L  +  G C     PCP F Y YG  G +
Sbjct: 143 SATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYG-DGSL 202

Query: 210 TGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCFGCVGATYREPIGIAGFGRGLLSLPFQ 269
              L  D L             S  +  V  F FGC   T  EPIG+AGFGRG LSLP Q
Sbjct: 203 VAKLYSDSL-------------SLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQ 262

Query: 270 LGFSQ----KGFSHCFLPFKF-SNNPNFSSPLILGHLA------------------ISSK 329
           L          FS+C +   F S+     SPLILG                        K
Sbjct: 263 LAVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKK 322

Query: 330 DENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGT 389
                FT +L++P +P +Y + L+ I+IG  N          LR ID  G GG+++DSGT
Sbjct: 323 KNEFVFTEMLENPKHPYFYSVSLQGISIGKRN----IPAPAMLRRIDKNGGGGVVVDSGT 382

Query: 390 TYTHLPEPLYSQLISNLESVIS--YPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPS 449
           T+T LP   Y+ ++   +S +   + RA +VE ++G   CY +             ++P+
Sbjct: 383 TFTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN---------QTVKVPA 442

Query: 450 ITFHFL-NNVSVVLPQGNNFYAMA----APINSTVVKCLLYQSMDGVGDDNDSDDNGPAG 508
           +  HF  N  SV LP+ N FY              + CL+  +    G D      G   
Sbjct: 443 LVLHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMN----GGDESELRGGTGA 494

[Close]

BLAST of MELO3C021800 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 183.7 bits (465), Expect = 3.8e-46
Identity = 139/426 (32.63%), Postives = 194/426 (45.54%), Query Frame = 0


Query: 89  GYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPKLAAFLPTHS 148
           GY +SLS GTP Q +    DTGS L W+PC +  + C  C+   + +    +  F+P +S
Sbjct: 89  GYSVSLSFGTPSQTIPFVFDTGSSLVWLPCTS-RYLCSGCD--FSGLDPTLIPRFIPKNS 148

Query: 149 STSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGTCPRPCPSFAYTYGASGVVTG 208
           S+S    C S  C  ++    P   C   GC   T     C   CP +   YG       
Sbjct: 149 SSSKIIGCQSPKCQFLY---GPNVQC--RGCDPNT---RNCTVGCPPYILQYGLGSTAGV 208

Query: 209 SLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCFGCVGATYREPIGIAGFGRGLLSLPFQLG 268
            +T  + F               +  VP F  GC   + R+P GIAGFGRG +SLP Q+ 
Sbjct: 209 LITEKLDF--------------PDLTVPDFVVGCSIISTRQPAGIAGFGRGPVSLPSQMN 268

Query: 269 FSQKGFSHCFLPFKFSNNPNFSSPLIL----GHLAISSKDENLQFTPLLKSPIYPN---- 328
              K FSHC +  +F ++ N ++ L L    GH +  SK   L +TP  K+P   N    
Sbjct: 269 L--KRFSHCLVSRRF-DDTNVTTDLDLDTGSGHNS-GSKTPGLTYTPFRKNPNVSNKAFL 328

Query: 329 -YYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISN 388
            YYY+ L  I +G  +      + +K     T G+GG ++DSG+T+T +  P++  +   
Sbjct: 329 EYYYLNLRRIYVGRKH----VKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEE 388

Query: 389 LESVIS-YPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSITFHFLNNVSVVLPQGN 448
             S +S Y R K +E  TG   C+ +  K        D  +P + F F     + LP  N
Sbjct: 389 FASQMSNYTREKDLEKETGLGPCFNISGKG-------DVTVPELIFEFKGGAKLELPLSN 448

Query: 449 NFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNLQVVYDLEKERLG 505
            F      + +T   CL   S   V   N S   GPA I GSFQQQN  V YDLE +R G
Sbjct: 449 YF----TFVGNTDTVCLTVVSDKTV---NPSGGTGPAIILGSFQQQNYLVEYDLENDRFG 467

[Close]

BLAST of MELO3C021800 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 149.8 bits (377), Expect = 6.0e-36
Identity = 142/513 (27.68%), Postives = 201/513 (39.18%), Query Frame = 0


Query: 13  FLSLFLL--------LVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLT-PKKGYNFIS 72
           FLSLFLL          H     L    K+ FP  +  L L   R   L+  +K   F+ 
Sbjct: 11  FLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLSLRRKPIPFVK 70

Query: 73  KKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSF 132
              +       G              Y + L IG PPQ + +  DTGSDL WV C     
Sbjct: 71  SPVVSGAASGSGQ-------------YFVDLRIGQPPQSLLLIADTGSDLVWVKCS---- 130

Query: 133 DCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLAT 192
            C++C  +           F P HSST     C    C  +   D        A     T
Sbjct: 131 ACRNCSHHS------PATVFFPRHSSTFSPAHCYDPVCRLVPKPDR-------APICNHT 190

Query: 193 LVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCFGC- 252
            +  TC      + Y Y    + +G   R+   +          +S    ++    FGC 
Sbjct: 191 RIHSTC-----HYEYGYADGSLTSGLFARETTSL--------KTSSGKEARLKSVAFGCG 250

Query: 253 --------VGATYREPIGIAGFGRGLLSLPFQLG--FSQKGFSHCFLPFKFSNNPNFSSP 312
                    G ++    G+ G GRG +S   QLG  F  K FS+C + +  S  P  +S 
Sbjct: 251 FRISGQSVSGTSFNGANGVMGLGRGPISFASQLGRRFGNK-FSYCLMDYTLSPPP--TSY 310

Query: 313 LILGHLAISSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTK 372
           LI+G+         L FTPLL +P+ P +YY+ L+S+ +    N  +  +   + EID  
Sbjct: 311 LIIGN--GGDGISKLFFTPLLTNPLSPTFYYVKLKSVFV----NGAKLRIDPSIWEIDDS 370

Query: 373 GNGGMLIDSGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSS 432
           GNGG ++DSGTT   L EP Y  +I+ +   +  P A    L  GFDLC  V     +  
Sbjct: 371 GNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD--ALTPGFDLCVNV-----SGV 430

Query: 433 FVDDSQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMD-GVGDDNDSDD 492
              +  LP + F F      V P  N F           ++CL  QS+D  VG       
Sbjct: 431 TKPEKILPRLKFEFSGGAVFVPPPRNYFIE-----TEEQIQCLAIQSVDPKVG------- 449

Query: 493 NGPAGIFGSFQQQNLQVVYDLEKERLGFQAMDC 505
                + G+  QQ     +D ++ RLGF    C
Sbjct: 491 ---FSVIGNLMQQGFLFEFDRDRSRLGFSRRGC 449

[Close]

BLAST of MELO3C021800 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 148.7 bits (374), Expect = 1.3e-35
Identity = 160/531 (30.13%), Postives = 227/531 (42.75%), Query Frame = 0


Query: 3   SISSTSIATKFLSLF--LLLVHASKQTL--ATNPKTNFPKDSLVLGLVH-----SRTSLL 62
           S SS+ +   FL LF  L+ V +S+++L   T PK N P+    L L H     + T + 
Sbjct: 4   SSSSSLLFPFFLILFSCLISVSSSRRSLIDRTLPK-NLPRSGFRLSLRHVDSGKNLTKIQ 63

Query: 63  TPKKGYN--FISKKRMKAM------DQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQV 122
             ++G N  F    R+ A+       + D  +N+  P       +LM LSIG P      
Sbjct: 64  KIQRGINRGFHRLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSA 123

Query: 123 YMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIH 182
            +DTGSDL W  C      C +C +    I       F P  SS+  +  C S  C  + 
Sbjct: 124 IVDTGSDLIWTQCK----PCTECFDQPTPI-------FDPEKSSSYSKVGCSSGLCNALP 183

Query: 183 SSDNPFDPCTIAGCSLATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNN 242
            S+   D             K  C      + YTYG      G L  +       + + N
Sbjct: 184 RSNCNED-------------KDAC-----EYLYTYGDYSSTRGLLATETF----TFEDEN 243

Query: 243 NNNSNNNKQVPRFCFGC----VGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPF 302
           +        +    FGC     G  + +  G+ G GRG LSL  QL   +  FS+C    
Sbjct: 244 S--------ISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQL--KETKFSYCLTSI 303

Query: 303 KFSNNPNFSSPLILGHLA--------ISSKDENLQFTPLLKSPIYPNYYYIGLESITIGN 362
           + S     SS L +G LA         S   E  +   LL++P  P++YY+ L+ IT+G 
Sbjct: 304 EDS---EASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQGITVGA 363

Query: 363 GNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLESVISYPRAKQVE 422
                R  V     E+   G GGM+IDSGTT T+L E  +  L     S +S P      
Sbjct: 364 K----RLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLP--VDDS 423

Query: 423 LNTGFDLCYKVPCKNNNSSFVDDSQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVK 482
            +TG DLC+K+P    N +      +P + FHF     + LP G N+  M A  +ST V 
Sbjct: 424 GSTGLDLCFKLPDAAKNIA------VPKMIFHF-KGADLELP-GENY--MVAD-SSTGVL 458

Query: 483 CLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNLQVVYDLEKERLGFQAMDC 505
           CL   S +G+             IFG+ QQQN  V++DLEKE + F   +C
Sbjct: 484 CLAMGSSNGM------------SIFGNVQQQNFNVLHDLEKETVSFVPTEC 458

The following BLAST results are available for this feature:

BLAST of MELO3C021800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	6.9e-53	33.26	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q766C2	7.2e-34	28.70	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q766C3	2.5e-31	28.99	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q9LNJ3	1.7e-30	28.08	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q6XBF8	1.3e-27	27.56	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]

BLAST of MELO3C021800 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008459091.1	0.0e+00	100.00	PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo] >KAA0043307.1 aspart...	[more]
XP_004145478.2	3.6e-293	95.19	probable aspartyl protease At4g16563 [Cucumis sativus] >KGN66888.1 hypothetical ...	[more]
XP_038893627.1	7.6e-267	88.48	probable aspartyl protease At4g16563 [Benincasa hispida]	[more]
XP_022142611.1	1.1e-238	79.51	probable aspartyl protease At4g16563 [Momordica charantia]	[more]
XP_023520027.1	1.7e-237	80.19	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MELO3C021800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TNC9	0.0e+00	100.00	Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3CAK9	0.0e+00	100.00	aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103498305 PE=3 S...	[more]
A0A0A0LYP0	1.8e-293	95.19	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G70459...	[more]
A0A6J1CMP8	5.5e-239	79.51	probable aspartyl protease At4g16563 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A6J1EHM1	2.0e-236	80.00	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114342...	[more]

BLAST of MELO3C021800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45120.1	1.4e-170	59.61	Eukaryotic aspartyl protease family protein	[more]
AT4G16563.1	4.9e-54	33.26	Eukaryotic aspartyl protease family protein	[more]
AT3G52500.1	3.8e-46	32.63	Eukaryotic aspartyl protease family protein	[more]
AT3G25700.1	6.0e-36	27.68	Eukaryotic aspartyl protease family protein	[more]
AT2G03200.1	1.3e-35	30.13	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 297..510 e-value: 7.5E-45 score: 154.8
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 84..296 e-value: 5.4E-30 score: 106.7
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 89..506
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 322..500 e-value: 3.7E-26 score: 91.8
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 90..296 e-value: 7.9E-28 score: 97.9
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 50..509
None	No IPR available	PANTHER	PTHR47967:SF47	CHLOROPLAST NUCLEOID DNA-BINDING PROTEIN-LIKE	coord: 50..509
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 357..368
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 90..500 score: 34.599976
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 89..504 e-value: 9.912E-69 score: 219.827

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C021800.1	MELO3C021800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity