MELO3C021208.jh1 (gene) Melon (Harukei-3) v1.41

BLAST of MELO3C021208.jh1 vs. NCBI nr
Match: XP_008458227.1 (PREDICTED: U-box domain-containing protein 33-like [Cucumis melo] >TYK03015.1 U-box domain-containing protein 33-like [Cucumis melo var. makuwa])

HSP 1 Score: 1477 bits (3825), Expect = 0.0
Identity = 769/836 (91.99%), Postives = 779/836 (93.18%), Query Frame = 0

Query: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISM 60
           MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAK IPI         
Sbjct: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPI--------- 60

Query: 61  CSLNIAQSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL 120
                        +GTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL
Sbjct: 61  -------------LGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL 120

Query: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS-SYSMHTSCTLLCIWSTAEDSDEMM- 180
           YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS   +   S   + +   A  S ++  
Sbjct: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQF 180

Query: 181 ---------------EAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLT 240
                          EAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLT
Sbjct: 181 VCKGQLIRIREACPQEAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLT 240

Query: 241 ISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPH 300
           ISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPH
Sbjct: 241 ISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPH 300

Query: 301 LSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSETLY 360
           LSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDE+ICWAKVSETLY
Sbjct: 301 LSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDEAICWAKVSETLY 360

Query: 361 AEESRGRKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVKELE 420
           AEESRGRK+AEEALSKEREELDNVKNQVNEMMKDLQIA+NNELKLENQIAQSDEMVKELE
Sbjct: 361 AEESRGRKEAEEALSKEREELDNVKNQVNEMMKDLQIAQNNELKLENQIAQSDEMVKELE 420

Query: 421 QKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVE 480
           QKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVE
Sbjct: 421 QKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVE 480

Query: 481 ATRKFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNL 540
           ATRKF PSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNL
Sbjct: 481 ATRKFDPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNL 540

Query: 541 ATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKI 600
           ATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKI
Sbjct: 541 ATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKI 600

Query: 601 CKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTT 660
           CKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTT
Sbjct: 601 CKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTT 660

Query: 661 AFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLAL 720
           AFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLAL
Sbjct: 661 AFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLAL 720

Query: 721 RCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMED 780
           RCCNTDSTYRPDL+SDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMED
Sbjct: 721 RCCNTDSTYRPDLVSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMED 780

Query: 781 PHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 819
           PHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ
Sbjct: 781 PHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814

BLAST of MELO3C021208.jh1 vs. NCBI nr
Match: XP_031743838.1 (U-box domain-containing protein 33 [Cucumis sativus] >KGN45661.1 hypothetical protein Csa_004782 [Cucumis sativus])

HSP 1 Score: 1362 bits (3524), Expect = 0.0
Identity = 717/837 (85.66%), Postives = 747/837 (89.25%), Query Frame = 0

Query: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISM 60
           MAGS GED VYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAK IPI         
Sbjct: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPI--------- 60

Query: 61  CSLNIAQSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL 120
                        +GTKFLASSM+EEEVNAYRELERQDA+KILDVFLLLCHQAGA+AEKL
Sbjct: 61  -------------LGTKFLASSMKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKL 120

Query: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS-SYSMHTSCTLLCIWSTAEDSDEMM- 180
           YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS   +   S   + +   A  S ++  
Sbjct: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQF 180

Query: 181 ---------------EAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLT 240
                          E HADISPSSPQSQNINGVSWRT+QFG FNGRISNSP+IVMERLT
Sbjct: 181 VCKGQLIRIREACPQETHADISPSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLT 240

Query: 241 ISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSP- 300
           ISETISNASG  SPFERSYATSPSSG  DVASSR+EED+YGLGL+SPL LTNFA  TSP 
Sbjct: 241 ISETISNASG--SPFERSYATSPSSGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPP 300

Query: 301 HLSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSETL 360
           H SGFQQDG+A+DSLYIQLEKAI DAANARREAFREALKRAKAEKELDE+ICWAKVSETL
Sbjct: 301 HFSGFQQDGSADDSLYIQLEKAIADAANARREAFREALKRAKAEKELDEAICWAKVSETL 360

Query: 361 YAEESRGRKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVKEL 420
           YAEESRGRKDAEE LSKEREELD+VKNQVNEMMK+LQIARNN LKLENQIAQSDEMVKEL
Sbjct: 361 YAEESRGRKDAEEELSKEREELDDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKEL 420

Query: 421 EQKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIV 480
           EQKILSAI+LLHNYKNDRDELLKQRDEAL+ELDDIR RQVEA SQ SAQLISEFSFSEIV
Sbjct: 421 EQKILSAIELLHNYKNDRDELLKQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIV 480

Query: 481 EATRKFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPN 540
           EATRKF PSLKIV   NGSMYKGLLYNTEVSIKMLCSHNLQNPV+FQREVDALSKLRHPN
Sbjct: 481 EATRKFDPSLKIVTDANGSMYKGLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPN 540

Query: 541 LATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNK 600
           +ATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIAT LCSALIFIHSNK
Sbjct: 541 IATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNK 600

Query: 601 ICKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLT 660
           ICK VHGDMKPSN+LLDANYVPKLAGFG CHFLTHDEK SYNENLSVG+DA  NHEFPLT
Sbjct: 601 ICKTVHGDMKPSNILLDANYVPKLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLT 660

Query: 661 TAFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLA 720
           T  D+YSFG VLLSLLTG+SYLRM+EDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLA
Sbjct: 661 TELDIYSFGTVLLSLLTGKSYLRMKEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLA 720

Query: 721 LRCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVME 780
           LRCC+TDS YRPDL+SD WRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVME
Sbjct: 721 LRCCDTDSMYRPDLVSDVWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVME 780

Query: 781 DPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 819
           DPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ
Sbjct: 781 DPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 813

BLAST of MELO3C021208.jh1 vs. NCBI nr
Match: XP_038907095.1 (U-box domain-containing protein 33-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1267 bits (3279), Expect = 0.0
Identity = 674/840 (80.24%), Postives = 716/840 (85.24%), Query Frame = 0

Query: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISM 60
           MAGS  ED VYVAVGNDI  CKLNLVYAIK SGGR+ICILHVHEPAK IPI         
Sbjct: 1   MAGSSEEDVVYVAVGNDIKACKLNLVYAIKQSGGRKICILHVHEPAKLIPI--------- 60

Query: 61  CSLNIAQSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL 120
                        +GTKFLASSM+EE+VNAYRELERQDANKILD FLLLCHQAGA+AEKL
Sbjct: 61  -------------LGTKFLASSMKEEKVNAYRELERQDANKILDGFLLLCHQAGARAEKL 120

Query: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS----------SYSMHTSCTLLCIWST 180
           YFESDKIKKGIVELVSLHGIR L+MGAAADKY+S          +  +H +    C    
Sbjct: 121 YFESDKIKKGIVELVSLHGIRRLIMGAAADKYHSRKMTKIRSRKAMYVHLNAPAFCQIQF 180

Query: 181 AEDSDEM-------MEAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLT 240
             +   +        E HADIS  SPQSQNING SWR  Q GQFNGRI +SPSI MERLT
Sbjct: 181 VCNGQLIRIREACPQEPHADISLPSPQSQNINGASWRPVQSGQFNGRIIDSPSIGMERLT 240

Query: 241 ISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSP- 300
           IS+ I N SGTWS FE SY+TSPSSGC DVASSR+EED + LGL SP +LTNFAPN+SP 
Sbjct: 241 ISDAILNTSGTWSAFEHSYSTSPSSGCMDVASSRTEEDEFELGLDSPRQLTNFAPNSSPP 300

Query: 301 HLSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSETL 360
           HL GFQQDG+A+DSLYIQLEKAITDAANARREAFREALKRAKAE EL  +I  AKV+E L
Sbjct: 301 HLLGFQQDGSADDSLYIQLEKAITDAANARREAFREALKRAKAEIELGHAIRRAKVAEAL 360

Query: 361 YAEESRGRKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVKEL 420
           YAEE RGRK+ EEA SKEREELD+VKNQVNEM K+LQIARN  LKLENQIA+SDEMVKEL
Sbjct: 361 YAEEFRGRKETEEARSKEREELDDVKNQVNEMTKELQIARNKGLKLENQIAESDEMVKEL 420

Query: 421 EQKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIV 480
           EQKILSAI+LLHNYKNDRDELLKQRDEAL+EL+DIR RQVEAR Q SA    EFSFSEI 
Sbjct: 421 EQKILSAIELLHNYKNDRDELLKQRDEALKELNDIRTRQVEARIQHSAH---EFSFSEIA 480

Query: 481 EATRKFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPV-EFQREVDALSKLRHP 540
           EATRKF PSLKIVEG NGSMYKGLLYNTEVSIKMLCSHNLQNP  EFQREVD LSKLRHP
Sbjct: 481 EATRKFDPSLKIVEGTNGSMYKGLLYNTEVSIKMLCSHNLQNPEDEFQREVDVLSKLRHP 540

Query: 541 NLATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSN 600
           N+ATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRI IAT+LCSALIFIHSN
Sbjct: 541 NIATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRIRIATELCSALIFIHSN 600

Query: 601 KICKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPL 660
           K+CKI+HGDMKPSNVLLDANYVPKLAGFG CHFL HDEKSSYNE+LS G+DA GNHEFPL
Sbjct: 601 KVCKIIHGDMKPSNVLLDANYVPKLAGFGTCHFLPHDEKSSYNEDLSAGYDAKGNHEFPL 660

Query: 661 TTAFDVYSFGMVLLSLLTGQSYLRMEEDVQ--IAIKKRKLKDVLDPRAGDWPFVQAEQLA 720
           TT  DV+SFGM+LLSLLTGQSYLR++EDVQ  I IKKRKLKDVLDPRAGDWPFVQAEQLA
Sbjct: 661 TTELDVFSFGMILLSLLTGQSYLRLKEDVQFIINIKKRKLKDVLDPRAGDWPFVQAEQLA 720

Query: 721 QLALRCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQE 780
           QLALRCCNT+S YRPDL+SD WRVLEPMRASCGGS SVCLSFGDQQVQPPPYFICPIFQE
Sbjct: 721 QLALRCCNTNSMYRPDLVSDVWRVLEPMRASCGGSQSVCLSFGDQQVQPPPYFICPIFQE 780

Query: 781 VMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 819
           VMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ
Sbjct: 781 VMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 815

BLAST of MELO3C021208.jh1 vs. NCBI nr
Match: XP_022959086.1 (U-box domain-containing protein 33-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1194 bits (3088), Expect = 0.0
Identity = 624/839 (74.37%), Postives = 698/839 (83.19%), Query Frame = 0

Query: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISM 60
           MAGS G+D VY+AVGND+NEC+LNLVYAIKH GGRRIC++HVHEPAK IP          
Sbjct: 1   MAGSCGDDVVYIAVGNDVNECRLNLVYAIKHLGGRRICVVHVHEPAKLIPF--------- 60

Query: 61  CSLNIAQSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL 120
                        +GT+FLASSM+E+EVNAYRE ERQDANKILD FL LCHQAG  AEKL
Sbjct: 61  -------------LGTQFLASSMKEKEVNAYRERERQDANKILDGFLFLCHQAGVWAEKL 120

Query: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS----------SYSMHTSCTLLC---- 180
           YFESDKIKKGIVELVSLHGIR L+MGAAADKYYS          +  +H      C    
Sbjct: 121 YFESDKIKKGIVELVSLHGIRRLIMGAAADKYYSRKMTKIRSRKAMYVHLKAPAFCQIQF 180

Query: 181 -----IWSTAEDSDEMMEAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMER 240
                +  T E   +  EAH D+ P SPQSQN+NG  WR  + GQFNG    SPSIV+ER
Sbjct: 181 VCNGQLIRTREACPQ--EAHVDVPPPSPQSQNVNGACWRPMESGQFNGSRIQSPSIVLER 240

Query: 241 LTISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTS 300
           L+IS+ +SN SGT SPFERSY+ S SSGC D ASSR+EED YG+GL+SPL L + APN+S
Sbjct: 241 LSISKLVSNDSGTSSPFERSYSPSSSSGCLDAASSRTEEDEYGVGLNSPLLLKDVAPNSS 300

Query: 301 P-HLSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSE 360
           P HLSGFQQD +A+D LYIQLEK+IT+AANA+REAFREA+KRA+AE EL+ +I  AK  E
Sbjct: 301 PLHLSGFQQDESADDILYIQLEKSITEAANAKREAFREAVKRAQAENELENAIFRAKFYE 360

Query: 361 TLYAEESRGRKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVK 420
            LYA E+R RKD EEALSKEREEL+NVKNQVN+MM++LQ++RN  L+LENQIA SD+MVK
Sbjct: 361 DLYAGEARERKDVEEALSKEREELENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVK 420

Query: 421 ELEQKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSE 480
           ELEQKILSAI+LLHNYKNDRDELLKQRDEAL+E+DDIR RQVEAR++ +AQ+ SEFSFSE
Sbjct: 421 ELEQKILSAIELLHNYKNDRDELLKQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSE 480

Query: 481 IVEATRKFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRH 540
           I EATRKF PSLKI EG +GSMYKGLLYNTEV IKM  SHNLQNPVEFQREVD LS LRH
Sbjct: 481 IAEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHNLQNPVEFQREVDVLSILRH 540

Query: 541 PNLATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHS 600
           PN+ATLIGVCPEACILVYDYFPNG+LEDRLACKDNSSPLSWKTRI IA++LCSALIFIHS
Sbjct: 541 PNIATLIGVCPEACILVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHS 600

Query: 601 NKICKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFP 660
           NKICKI+HGD+KP NVLLDANYVPKLAGFG CHFL HDEK SYNEN+S  +    N EFP
Sbjct: 601 NKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENISAKYGVKSNPEFP 660

Query: 661 LTTAFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQ 720
           LTT  DV+SFGMVLLSLLTG+SYLRM+EDVQ AI++RKLKDVLDPRAGDWPFVQAEQLAQ
Sbjct: 661 LTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAIEERKLKDVLDPRAGDWPFVQAEQLAQ 720

Query: 721 LALRCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEV 780
           LALRCCN +S YRPDL+SD WRVLEPM+ASCGGS+SV LSFGD QVQPPPYF+CPIFQEV
Sbjct: 721 LALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEV 780

Query: 781 MEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 819
           MEDPHVAADGFTYEAEALRGWLDSGH+TSPMTNLRL+HQNLVPNRALRSVIQEWL Q Q
Sbjct: 781 MEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQQQ 815

BLAST of MELO3C021208.jh1 vs. NCBI nr
Match: KAG7013740.1 (U-box domain-containing protein 33 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1191 bits (3081), Expect = 0.0
Identity = 624/839 (74.37%), Postives = 696/839 (82.96%), Query Frame = 0

Query: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISM 60
           MAGS G+D VY+AVGND+NEC+LNLVYAIKH GGRRIC++HVHEPAK IP          
Sbjct: 1   MAGSCGDDVVYMAVGNDVNECRLNLVYAIKHLGGRRICVVHVHEPAKLIPF--------- 60

Query: 61  CSLNIAQSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL 120
                        +GT+FLASSM+E+EVNAYRE ERQDAN ILD FL LCHQAG  AEKL
Sbjct: 61  -------------LGTQFLASSMKEKEVNAYRERERQDANNILDGFLFLCHQAGVWAEKL 120

Query: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS----------SYSMHTSCTLLC---- 180
           YFESDKIKKGIVELVSLHGIR L+MGAAADKYYS          +  +H      C    
Sbjct: 121 YFESDKIKKGIVELVSLHGIRRLIMGAAADKYYSRKMTKIRSRKAMYVHLKAPAFCQIQF 180

Query: 181 -----IWSTAEDSDEMMEAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMER 240
                +  T E   +  EAH D+ P SPQSQN+NG  WR  + GQFNG    SPSIV+ER
Sbjct: 181 VCNGQLIRTREACPQ--EAHVDVPPPSPQSQNVNGACWRPMESGQFNGSRIQSPSIVLER 240

Query: 241 LTISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTS 300
           L+IS+ +SN SGT SPFERSY+ S SSGC D ASSR+EED YG+GL+SPL L + APN+S
Sbjct: 241 LSISKLVSNDSGTSSPFERSYSPSSSSGCLDAASSRTEEDEYGVGLNSPLLLKDVAPNSS 300

Query: 301 P-HLSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSE 360
           P HLSGFQQD +A+D LYIQLEK+IT+AANA+REAFREA+KRA+AE EL  +I  AK  E
Sbjct: 301 PLHLSGFQQDESADDILYIQLEKSITEAANAKREAFREAVKRAQAENELGNAIFRAKFYE 360

Query: 361 TLYAEESRGRKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVK 420
            LYA E+R RKD EEALSKEREEL+NVKNQVN+MM++LQ++RN  L+LENQIA SD+MVK
Sbjct: 361 DLYAGEARERKDVEEALSKEREELENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVK 420

Query: 421 ELEQKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSE 480
           ELEQKILSAI+LLHNYKNDRDELLKQRDEAL+E+DDIR RQVEARS+ +AQ+ SEFSFSE
Sbjct: 421 ELEQKILSAIELLHNYKNDRDELLKQRDEALKEVDDIRTRQVEARSKHTAQVFSEFSFSE 480

Query: 481 IVEATRKFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRH 540
           I EATRKF PSLKI EG +GSMYKGLLYNTEV IKM  SHNLQNPVEFQREVD LS LRH
Sbjct: 481 IAEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHNLQNPVEFQREVDVLSILRH 540

Query: 541 PNLATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHS 600
           PN+ATLIGVCPEACILVYDYFPNG+LEDRLACKDNSSPLSWKTRI IA++LCSALIFIHS
Sbjct: 541 PNIATLIGVCPEACILVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHS 600

Query: 601 NKICKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFP 660
           NKICKI+HGD+KP NVLLDANYVPKLAGFG CHFL HDEK SYNEN+S  +    N EFP
Sbjct: 601 NKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENISAKYGVKSNPEFP 660

Query: 661 LTTAFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQ 720
           LTT  DV+SFGMVLLSLLTG+SYLRM+EDVQ AI++RKLKDVLDPRAGDWPFVQAEQLAQ
Sbjct: 661 LTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAIEERKLKDVLDPRAGDWPFVQAEQLAQ 720

Query: 721 LALRCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEV 780
           LALRCCN +S YRPDL+SD WRVLEPM+ASCGGS+SV LSFGD QVQPPPYF+CPIFQEV
Sbjct: 721 LALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEV 780

Query: 781 MEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 819
           MEDPHVAADGFTYEAEALRGWLDSGH+TSPMTNLRL+HQNLVPNRALRSVIQEWL Q Q
Sbjct: 781 MEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQQQ 815

BLAST of MELO3C021208.jh1 vs. ExPASy Swiss-Prot
Match: Q8GUH1 (U-box domain-containing protein 33 OS=Arabidopsis thaliana OX=3702 GN=PUB33 PE=2 SV=2)

HSP 1 Score: 645.6 bits (1664), Expect = 7.6e-184
Identity = 370/835 (44.31%), Postives = 528/835 (63.23%), Query Frame = 0

Query: 7   EDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISMCSLNIA 66
           ++ ++VAV   + + K  L++A++++GG++IC++HVH+P++ IP                
Sbjct: 44  DEKIFVAVDKHVAKSKSTLIWALQNTGGKKICLIHVHQPSQMIP---------------- 103

Query: 67  QSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKLYFESDK 126
                 ++G KF   +++EEEV  +RE ER+  + ILD +L +C Q G +AEK++ E + 
Sbjct: 104 ------LMGAKFPVGAVKEEEVRVFREKEREKVHMILDDYLRICQQRGVRAEKMFIEMES 163

Query: 127 IKKGIVELVSLHGIRSLVMGAAADKYYSS-----------YSMHTSCTLLCIWSTAE--- 186
           I+ GIV+L+S  GIR LVMGAAAD++YS            +    + TL  IW T +   
Sbjct: 164 IENGIVQLISELGIRKLVMGAAADRHYSRRMTDLKSRKAIFVRREAPTLCQIWFTCKGYL 223

Query: 187 ------DSDEMMEAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLTISE 246
                   D+    +A   PS   S  +   S    +  Q   R+ ++ S       + +
Sbjct: 224 IHTREATMDDTESEYASPRPSISASDLLQTFSTPESEH-QHISRVQSTDS-------VQQ 283

Query: 247 TISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPHLSG 306
            +SN S T    E+S   S  S  TD    R  + S   G ++ +   + +P++ P    
Sbjct: 284 LVSNGSST----EQSGRVSDGSLNTD-EEERESDGSEVTGSATVMSSGHSSPSSFP---- 343

Query: 307 FQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSETLYAEE 366
              DG  +DS  +++ KA ++A ++++EAF E L+R KAEK   ++I  AK SE+ Y+EE
Sbjct: 344 ---DG-VDDSFNVKIRKATSEAHSSKQEAFAETLRRQKAEKNALDAIRRAKQSESAYSEE 403

Query: 367 SRGRKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVKELEQKI 426
            + RKD E A++KE+E    +KN+   +M++LQ A   +  LE+QIA+SD  +++L QK+
Sbjct: 404 LKRRKDTEIAVAKEKERFITIKNEQEVIMEELQSAMAQKAMLESQIAKSDGTMEKLNQKL 463

Query: 427 LSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVEATR 486
             A++LL   +++R+EL  +RD AL+E +++R    E  + +  Q  ++FSFSEI EAT 
Sbjct: 464 DIAVKLLQKLRDEREELQTERDRALREAEELRS-HAETSTLQLPQYFTDFSFSEIEEATN 523

Query: 487 KFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATL 546
            F  +LKI EG  GS+Y GLL +T+V+IKML  ++ Q PVE+Q+EVD LSK+RHPN+ TL
Sbjct: 524 HFDSTLKIGEGGYGSIYVGLLRHTQVAIKMLNPNSSQGPVEYQQEVDVLSKMRHPNIITL 583

Query: 547 IGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKI 606
           IG CPE   LVY+Y P G+LEDRL CKDNS PLSW+ R+ IAT++C+AL+F+HSNK   +
Sbjct: 584 IGACPEGWSLVYEYLPGGSLEDRLTCKDNSPPLSWQNRVRIATEICAALVFLHSNKAHSL 643

Query: 607 VHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENL--SVGH-DAMGNHEFPLTT 666
           VHGD+KP+N+LLD+N V KL+ FG C  L  +   S   ++  +V + D   +    LT 
Sbjct: 644 VHGDLKPANILLDSNLVSKLSDFGTCSLLHPNGSKSVRTDVTGTVAYLDPEASSSGELTP 703

Query: 667 AFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLAL 726
             DVYSFG++LL LLTG+  LR+  +V+ A+    L D+LDP AGDWPFVQAEQLA+LAL
Sbjct: 704 KSDVYSFGIILLRLLTGRPALRISNEVKYALDNGTLNDLLDPLAGDWPFVQAEQLARLAL 763

Query: 727 RCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMED 786
           RCC T S  RPDL ++ WRVLEPMRAS GGS S  L   + ++  PPYFICPIFQEVM+D
Sbjct: 764 RCCETVSENRPDLGTEVWRVLEPMRASSGGSSSFHLGRNEHRI-APPYFICPIFQEVMQD 823

Query: 787 PHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQH 819
           PHVAADGFTYEAEA+R WLDS HDTSPMTN++L H +L+ N ALRS IQEWLQ H
Sbjct: 824 PHVAADGFTYEAEAIRAWLDSEHDTSPMTNVKLSHTSLIANHALRSAIQEWLQHH 833

BLAST of MELO3C021208.jh1 vs. ExPASy Swiss-Prot
Match: Q94A51 (U-box domain-containing protein 32 OS=Arabidopsis thaliana OX=3702 GN=PUB32 PE=1 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.2e-120
Identity = 284/825 (34.42%), Postives = 459/825 (55.64%), Query Frame = 0

Query: 7   EDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISMCSLNIA 66
           ++ ++VAV  D+   K  +++A ++  G++IC+L+VH  A++                 A
Sbjct: 14  DETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARA-----------------A 73

Query: 67  QSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKLYFESDK 126
             ++  +VG      S ++ +V     +E+   +++++ +L L  +   Q +KL      
Sbjct: 74  SWTHKKLVG-----GSFKKHDVKVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQN 133

Query: 127 IKKGIVELVSLHGIRSLVMGAAADKYYS-SYSMHTSCTLLCIWSTAEDSDE---MMEAHA 186
           I++ IVEL++ H I+ LVMGAA+DK+YS   +   S   + +   A DS     + + + 
Sbjct: 134 IEECIVELIARHKIKWLVMGAASDKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYL 193

Query: 187 DISPSSPQSQNINGVSWRTDQFGQFNGRISN---SPSIVMERLTISETISNASG--TWSP 246
             + +S    N         Q    N    +     S +  RL    ++    G      
Sbjct: 194 IFTRASNDDSNNRQTMPPLVQLDSDNETRKSEKLESSYMRRRLRYWRSLLEQDGEKDTGQ 253

Query: 247 FERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPHLSGFQQDGNANDSL 306
            ER      +       SS S  +  G    SP  + +   NTS ++   +++G+    +
Sbjct: 254 LEREKVEPRAPPLFSSGSSSSFGEPVGPEPVSPELVDSDTLNTS-NVEEKEREGDVARKV 313

Query: 307 YIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSETLYAEESRGRKDAEEAL 366
           + + +KA+ D   + R  + EA K+ + +    E++C AK  E L  +ES  RK  EE L
Sbjct: 314 H-RYDKAMHDIGQSDRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKESSQRKRLEELL 373

Query: 367 SKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVKELEQKILSAIQLLHNYK 426
            KE+ E+  V  Q N  MK+LQ+ +   LKLE+Q+ +  ++ KE  +K  +A++LL +++
Sbjct: 374 EKEKLEVKMVIEQNNGFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKFDTAMELLKSFR 433

Query: 427 NDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVEATRKFVPSLKIVEG 486
             RDE+    + A++E++ +R+       + S   + ++SF EI EAT +F PS K+ EG
Sbjct: 434 QKRDEIRIDHENAVKEVNALRRLVKGETGESSGSEMLDYSFMEINEATNEFDPSWKLGEG 493

Query: 487 ENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATLIGVCPEACILV 546
           + GS+YKG L + +V++KML S+   N  EF+R V+ LS++RHPNL TL+G CPE+  L+
Sbjct: 494 KYGSVYKGNLQHLQVAVKMLPSYGSLNHFEFERRVEILSRVRHPNLVTLMGACPESRSLI 553

Query: 547 YDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKIVHGDMKPSNVL 606
           Y Y PNG+LED  + ++N   LSW++RI IA+++CSAL+F+HSN  C I+HG++KPS +L
Sbjct: 554 YQYIPNGSLEDCFSSENNVPALSWESRIRIASEICSALLFLHSNIPC-IIHGNLKPSKIL 613

Query: 607 LDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEF---PLTTAFDVYSFGMVL 666
           LD+N V K+  +GI   +  D        L      +  H F    +T   D+Y+FG++L
Sbjct: 614 LDSNLVTKINDYGISQLIPID-------GLDKSDPHVDPHYFVSREMTLESDIYAFGIIL 673

Query: 667 LSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCNTDSTYRP 726
           L LLT +    +  DV+ A++   +  VLD  AGDWP  + ++LA +A+RCC  +   RP
Sbjct: 674 LQLLTRRPVSGILRDVKCALENDNISAVLDNSAGDWPVARGKKLANVAIRCCKKNPMNRP 733

Query: 727 DLMSDAWRVLEPMRASCGGSLSVCLSFGDQQV--QPPPYFICPIFQEVMEDPHVAADGFT 786
           DL +   R ++ M+A    S S   S+ +Q V  +PP +++CPIFQEVM+DP +AADGFT
Sbjct: 734 DL-AVVLRFIDRMKAPEVPS-SETSSYANQNVPRRPPSHYLCPIFQEVMKDPLIAADGFT 793

Query: 787 YEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQ 818
           YEAEA+R WL +GHDTSPMTNL+++  NL+PN AL   IQ+W  Q
Sbjct: 794 YEAEAIREWLANGHDTSPMTNLKMEDCNLIPNHALHLAIQDWQNQ 804

BLAST of MELO3C021208.jh1 vs. ExPASy Swiss-Prot
Match: Q5WA76 (U-box domain-containing protein 70 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB70 PE=1 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 1.4e-113
Identity = 232/536 (43.28%), Postives = 331/536 (61.75%), Query Frame = 0

Query: 290 DGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSETLYAEESRG 349
           D + ND L  +L+K++ +AA  ++EA  E LKR ++E+        A+  E LY  + + 
Sbjct: 288 DLHLNDDLCNKLQKSMDEAAVLKKEASDERLKRIESER-------LARTLEDLYLSQVQQ 347

Query: 350 RKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVKELEQKILSA 409
           RK+ EE+LS+ ++E + +K Q +E+  +LQ        L  Q++ S E  + L       
Sbjct: 348 RKETEESLSRVQQEFEQLKIQQDEVTVELQRVNEQNENLLGQLSDSREHFEWL------- 407

Query: 410 IQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVEATRKFV 469
                   ++ D+LL++RD A++E++++R+++ +  S     +  EFS SE+  AT  F 
Sbjct: 408 -------LSEHDQLLRERDNAVREVEELRQKRGQMLSVLVTAMHCEFSSSEVESATENFS 467

Query: 470 PSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATLIGV 529
            SLKI EG  G +YKG+L N  V+IK+L   +LQ   +F++EV  LS++RHP+L TL+G 
Sbjct: 468 NSLKIGEGGFGCVYKGILRNMTVAIKVLRPDSLQGQSQFEQEVSILSRVRHPHLVTLLGA 527

Query: 530 CPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKIVHG 589
           C E+  LVY++ PNG+LED L C D    L+W+ RI I  ++CSALIF+H NK   +VHG
Sbjct: 528 CSESSTLVYEFLPNGSLEDFLMCSDKRQTLTWQARIRIIAEICSALIFLHKNKPHPVVHG 587

Query: 590 DMKPSNVLLDANYVPKLAGFGICHFLTHDEKSS---YNENLSVGHDAMGNHEF----PLT 649
           D+KP+N+LL  N V KL+ FGI   L     ++   Y     VG     + EF     LT
Sbjct: 588 DLKPANILLGVNLVSKLSDFGISRLLIQSSTNNTTLYRTMHPVGTPLYMDPEFLSTGELT 647

Query: 650 TAFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLA 709
              DVYSFG+V+L LLTG+  + ++  V+ A++K  L  V+D   G+WP +  EQLA LA
Sbjct: 648 PQSDVYSFGIVVLRLLTGKPPVGIKNIVEDAMEKGDLNSVIDTSVGEWPHLHIEQLAYLA 707

Query: 710 LRCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVME 769
           LRC       RPDL  + W ++E +R +   S S   S  DQ   PP YFICPI Q++M+
Sbjct: 708 LRCTELSRRCRPDLSGEVWAIVEAIRDAALSSPSSSRSAQDQN-SPPSYFICPISQDIMD 767

Query: 770 DPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQH 819
           DPH+AADGFTYEAEA+R WL +GHDTSPMTNL L+H+ L+PNRALRS IQEWLQQH
Sbjct: 768 DPHIAADGFTYEAEAIRSWLCNGHDTSPMTNLLLEHEELIPNRALRSAIQEWLQQH 801

BLAST of MELO3C021208.jh1 vs. ExPASy Swiss-Prot
Match: Q0DR28 (U-box domain-containing protein 57 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB57 PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 1.3e-87
Identity = 171/402 (42.54%), Postives = 242/402 (60.20%), Query Frame = 0

Query: 421 DELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVEATRKFVPSLKIVEGENG 480
           D+L  +RD A+Q+++++R         +S  +I +FS  ++ +AT  F  + ++ + E G
Sbjct: 120 DQLKHERDNAVQQVNELR--------DQSTHIILDFSRKDMEQATEHFKNAREVGDTEYG 179

Query: 481 SMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATLIGVCPEACILVYDY 540
             YKG+++N +V IK+  S  L     FQ+EV  L + RHPN+ T IGVC E   LVY++
Sbjct: 180 HTYKGMIHNMKVLIKLSSSQKL-----FQQEVSILRQWRHPNIITFIGVCSEVSALVYEW 239

Query: 541 FPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKIVHGDMKPSNVLLDA 600
            PNGNLEDR+ C +NS+PLSW  R  I  ++C AL+F+HSNK   +VHGD++P N+L+DA
Sbjct: 240 LPNGNLEDRIICTNNSAPLSWYNRTQIIGEICCALLFLHSNKSTALVHGDLRPCNILIDA 299

Query: 601 NYVPKLAGFGICHFLTHDEKSSYNENLSVG---HDAMGNHEFPLTTAFDVYSFGMVLLSL 660
           NY  K+  FG+ +     +  ++  NL+      D   N    LTT  DVYS G+++L L
Sbjct: 300 NYRSKICNFGMSNLFL--QLGTFPPNLTARLPYMDPEFNTTGELTTLSDVYSLGVIILRL 359

Query: 661 LTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCNTDSTYRPDLM 720
           LTG   L + E V  A+    L  ++D  AGDWP+++A+QLA + L C       RPDL+
Sbjct: 360 LTGMPPLTLSEKVAEALGSDSLHLLIDKSAGDWPYIEAKQLALIGLSCTGMTRKKRPDLL 419

Query: 721 SDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEA 780
           ++ W V+EP+      +    L         P  FICPI  E+M+DP VA+DGFTYEAEA
Sbjct: 420 NEVWIVIEPLTRKPPAATWPYLQSASGDSSVPAAFICPISMEIMKDPQVASDGFTYEAEA 479

Query: 781 LRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 820
           +R W D G   SPMTNL L + NLVPNR LRS I  +LQQ Q
Sbjct: 480 IRCWFDRGISRSPMTNLALPNLNLVPNRVLRSFIHGYLQQQQ 506

BLAST of MELO3C021208.jh1 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.8e-71
Identity = 231/782 (29.54%), Postives = 371/782 (47.44%), Query Frame = 0

Query: 85  EEEVNAYRELERQDANK-ILDVFLLLCHQAGAQAEKLYFESDKIKKGIVELVSLHGIRSL 144
           EE   AYR+   Q+  + +L  F  +C +   + ++  FES   K      V L  + S 
Sbjct: 90  EEVAAAYRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVA----VELQVLESN 149

Query: 145 VMGAAADKYYSSYSMHTSCTLLCIWSTAEDSDEMMEAHADISPSSPQSQNINGVSWRTDQ 204
            +  A  K     + H    L+   S+   S    +  A IS S      +  VS     
Sbjct: 150 SVAVAITK---EVNQHLISNLIIGRSSQAASSRNYDITASISASVSNLCTVYVVS----- 209

Query: 205 FGQFNGRISNSPSIVMERLTISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSY 264
               NG +      ++ + T S+T  N +   S FER+     SS C+  + + S+  S 
Sbjct: 210 ----NGGVH-----ILAKDT-SDTERNDTSIESGFERT-----SSSCSSGSGANSDVMSN 269

Query: 265 GLGLSSPLRLTNFAPNTSPHLSGFQQDGNANDSLYIQLEKAITDAANARR---EAFREAL 324
            L  S+P  L+N      P +            + + +E + T++   ++   +A  EA 
Sbjct: 270 AL-KSNPHTLSNKRMQNLPTIV---------RGVSVPMETSSTESDETKKRSSDAAEEAS 329

Query: 325 KRAKAEKELDESICWAKVSETLYAEESRGRKDAEEALSKEREELDNVKNQVNEMMKDLQI 384
           KR  +  E   S+ W          +   RKDA  ++S    E  NV   +     D Q 
Sbjct: 330 KR--SSPETSRSVSW-----NPQFRDFDERKDAMSSMS-SNFEYGNVVTPLGHYFTDNQD 389

Query: 385 ARNNELKLENQIAQSDEMVKELEQKILSAIQLLHNYKNDRDELLKQRDEAL-----QELD 444
             N   KL  ++  + EM    + + L A + L+  K +   LL+   + +     ++ +
Sbjct: 390 TLNEISKLRAELRHAHEMYAVAQVETLDASRKLNELKFEELTLLEHETKGIAKKETEKFE 449

Query: 445 DIRKRQVEARSQRSAQL-----------------------ISEFSFSEIVEATRKFVPSL 504
             R+ + EA  +R A++                         EF++ EI+ AT  F   L
Sbjct: 450 QKRREEREAAQRREAEMKATHEAKEKEKLEESSLVAPKLQYQEFTWEEIINATSSFSEDL 509

Query: 505 KIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATLIGVCPE 564
           KI  G  G +YK  L++T  ++K+L S       +F +E++ LSK+RHP+L  L+G CP+
Sbjct: 510 KIGMGAYGDVYKCNLHHTIAAVKVLHSAESSLSKQFDQELEILSKIRHPHLVLLLGACPD 569

Query: 565 ACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKIVHGDMK 624
              LVY+Y  NG+LEDRL   ++S P+ W  R+ IA ++ SAL+F+H +K   I+H D+K
Sbjct: 570 HGALVYEYMENGSLEDRLFQVNDSQPIPWFVRLRIAWEVASALVFLHKSKPTPIIHRDLK 629

Query: 625 PSNVLLDANYVPKLAGFGICHFLTHDEKSS-----YNENLSVGHDAMGNHEFPLTTAF-- 684
           P+N+LL+ N+V K+   G+   +   +  S     Y +   VG     + E+  T     
Sbjct: 630 PANILLNHNFVSKVGDVGLSTMIQAADPLSTKFTMYKQTSPVGTLCYIDPEYQRTGRISP 689

Query: 685 --DVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKR---KLKDVLDPRAGDWPFVQAEQLAQ 744
             DVY+FGM++L LLTGQ  + +   V+ A++     +L  +LD +AG+WP  +  QLA 
Sbjct: 690 KSDVYAFGMIILQLLTGQQAMALTYTVETAMENNNDDELIQILDEKAGNWPIEETRQLAA 749

Query: 745 LALRCCNTDSTYRPDLMSDAWRVLEPMRA---SCGGSLSVCLSFGDQQVQPPPYFICPIF 804
           LAL+C    S  RPDL      VLE ++        SLS   S      QPP +F CP+ 
Sbjct: 750 LALQCTELRSKDRPDLEDQILPVLESLKKVADKARNSLSAAPS------QPPSHFFCPLL 809

Query: 805 QEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQ 820
           ++VM++P +AADG+TY+  A+  W+++ H TSP+TN  L + NL+PN  L + I EW  +
Sbjct: 810 KDVMKEPCIAADGYTYDRRAIEEWMEN-HRTSPVTNSPLQNVNLLPNHTLYAAIVEWRNR 819

BLAST of MELO3C021208.jh1 vs. ExPASy TrEMBL
Match: A0A5D3BV84 (E3 ubiquitin ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001100 PE=4 SV=1)

HSP 1 Score: 1477 bits (3825), Expect = 0.0
Identity = 769/836 (91.99%), Postives = 779/836 (93.18%), Query Frame = 0

Query: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISM 60
           MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAK IPI         
Sbjct: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPI--------- 60

Query: 61  CSLNIAQSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL 120
                        +GTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL
Sbjct: 61  -------------LGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL 120

Query: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS-SYSMHTSCTLLCIWSTAEDSDEMM- 180
           YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS   +   S   + +   A  S ++  
Sbjct: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQF 180

Query: 181 ---------------EAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLT 240
                          EAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLT
Sbjct: 181 VCKGQLIRIREACPQEAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLT 240

Query: 241 ISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPH 300
           ISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPH
Sbjct: 241 ISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPH 300

Query: 301 LSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSETLY 360
           LSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDE+ICWAKVSETLY
Sbjct: 301 LSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDEAICWAKVSETLY 360

Query: 361 AEESRGRKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVKELE 420
           AEESRGRK+AEEALSKEREELDNVKNQVNEMMKDLQIA+NNELKLENQIAQSDEMVKELE
Sbjct: 361 AEESRGRKEAEEALSKEREELDNVKNQVNEMMKDLQIAQNNELKLENQIAQSDEMVKELE 420

Query: 421 QKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVE 480
           QKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVE
Sbjct: 421 QKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVE 480

Query: 481 ATRKFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNL 540
           ATRKF PSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNL
Sbjct: 481 ATRKFDPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNL 540

Query: 541 ATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKI 600
           ATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKI
Sbjct: 541 ATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKI 600

Query: 601 CKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTT 660
           CKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTT
Sbjct: 601 CKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTT 660

Query: 661 AFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLAL 720
           AFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLAL
Sbjct: 661 AFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLAL 720

Query: 721 RCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMED 780
           RCCNTDSTYRPDL+SDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMED
Sbjct: 721 RCCNTDSTYRPDLVSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMED 780

Query: 781 PHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 819
           PHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ
Sbjct: 781 PHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814

BLAST of MELO3C021208.jh1 vs. ExPASy TrEMBL
Match: A0A1S3C7H7 (E3 ubiquitin ligase OS=Cucumis melo OX=3656 GN=LOC103497714 PE=4 SV=1)

HSP 1 Score: 1477 bits (3825), Expect = 0.0
Identity = 769/836 (91.99%), Postives = 779/836 (93.18%), Query Frame = 0

Query: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISM 60
           MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAK IPI         
Sbjct: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPI--------- 60

Query: 61  CSLNIAQSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL 120
                        +GTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL
Sbjct: 61  -------------LGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL 120

Query: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS-SYSMHTSCTLLCIWSTAEDSDEMM- 180
           YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS   +   S   + +   A  S ++  
Sbjct: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQF 180

Query: 181 ---------------EAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLT 240
                          EAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLT
Sbjct: 181 VCKGQLIRIREACPQEAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLT 240

Query: 241 ISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPH 300
           ISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPH
Sbjct: 241 ISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPH 300

Query: 301 LSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSETLY 360
           LSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDE+ICWAKVSETLY
Sbjct: 301 LSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDEAICWAKVSETLY 360

Query: 361 AEESRGRKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVKELE 420
           AEESRGRK+AEEALSKEREELDNVKNQVNEMMKDLQIA+NNELKLENQIAQSDEMVKELE
Sbjct: 361 AEESRGRKEAEEALSKEREELDNVKNQVNEMMKDLQIAQNNELKLENQIAQSDEMVKELE 420

Query: 421 QKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVE 480
           QKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVE
Sbjct: 421 QKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVE 480

Query: 481 ATRKFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNL 540
           ATRKF PSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNL
Sbjct: 481 ATRKFDPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNL 540

Query: 541 ATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKI 600
           ATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKI
Sbjct: 541 ATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKI 600

Query: 601 CKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTT 660
           CKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTT
Sbjct: 601 CKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTT 660

Query: 661 AFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLAL 720
           AFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLAL
Sbjct: 661 AFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLAL 720

Query: 721 RCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMED 780
           RCCNTDSTYRPDL+SDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMED
Sbjct: 721 RCCNTDSTYRPDLVSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMED 780

Query: 781 PHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 819
           PHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ
Sbjct: 781 PHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 814

BLAST of MELO3C021208.jh1 vs. ExPASy TrEMBL
Match: A0A0A0KD01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004520 PE=4 SV=1)

HSP 1 Score: 1362 bits (3524), Expect = 0.0
Identity = 717/837 (85.66%), Postives = 747/837 (89.25%), Query Frame = 0

Query: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISM 60
           MAGS GED VYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAK IPI         
Sbjct: 1   MAGSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPI--------- 60

Query: 61  CSLNIAQSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL 120
                        +GTKFLASSM+EEEVNAYRELERQDA+KILDVFLLLCHQAGA+AEKL
Sbjct: 61  -------------LGTKFLASSMKEEEVNAYRELERQDADKILDVFLLLCHQAGARAEKL 120

Query: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS-SYSMHTSCTLLCIWSTAEDSDEMM- 180
           YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS   +   S   + +   A  S ++  
Sbjct: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQF 180

Query: 181 ---------------EAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLT 240
                          E HADISPSSPQSQNINGVSWRT+QFG FNGRISNSP+IVMERLT
Sbjct: 181 VCKGQLIRIREACPQETHADISPSSPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLT 240

Query: 241 ISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSP- 300
           ISETISNASG  SPFERSYATSPSSG  DVASSR+EED+YGLGL+SPL LTNFA  TSP 
Sbjct: 241 ISETISNASG--SPFERSYATSPSSGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPP 300

Query: 301 HLSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSETL 360
           H SGFQQDG+A+DSLYIQLEKAI DAANARREAFREALKRAKAEKELDE+ICWAKVSETL
Sbjct: 301 HFSGFQQDGSADDSLYIQLEKAIADAANARREAFREALKRAKAEKELDEAICWAKVSETL 360

Query: 361 YAEESRGRKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVKEL 420
           YAEESRGRKDAEE LSKEREELD+VKNQVNEMMK+LQIARNN LKLENQIAQSDEMVKEL
Sbjct: 361 YAEESRGRKDAEEELSKEREELDDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKEL 420

Query: 421 EQKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIV 480
           EQKILSAI+LLHNYKNDRDELLKQRDEAL+ELDDIR RQVEA SQ SAQLISEFSFSEIV
Sbjct: 421 EQKILSAIELLHNYKNDRDELLKQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIV 480

Query: 481 EATRKFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPN 540
           EATRKF PSLKIV   NGSMYKGLLYNTEVSIKMLCSHNLQNPV+FQREVDALSKLRHPN
Sbjct: 481 EATRKFDPSLKIVTDANGSMYKGLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPN 540

Query: 541 LATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNK 600
           +ATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIAT LCSALIFIHSNK
Sbjct: 541 IATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNK 600

Query: 601 ICKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLT 660
           ICK VHGDMKPSN+LLDANYVPKLAGFG CHFLTHDEK SYNENLSVG+DA  NHEFPLT
Sbjct: 601 ICKTVHGDMKPSNILLDANYVPKLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLT 660

Query: 661 TAFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLA 720
           T  D+YSFG VLLSLLTG+SYLRM+EDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLA
Sbjct: 661 TELDIYSFGTVLLSLLTGKSYLRMKEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLA 720

Query: 721 LRCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVME 780
           LRCC+TDS YRPDL+SD WRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVME
Sbjct: 721 LRCCDTDSMYRPDLVSDVWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVME 780

Query: 781 DPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 819
           DPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ
Sbjct: 781 DPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 813

BLAST of MELO3C021208.jh1 vs. ExPASy TrEMBL
Match: A0A6J1H3V9 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111460185 PE=4 SV=1)

HSP 1 Score: 1194 bits (3088), Expect = 0.0
Identity = 624/839 (74.37%), Postives = 698/839 (83.19%), Query Frame = 0

Query: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISM 60
           MAGS G+D VY+AVGND+NEC+LNLVYAIKH GGRRIC++HVHEPAK IP          
Sbjct: 1   MAGSCGDDVVYIAVGNDVNECRLNLVYAIKHLGGRRICVVHVHEPAKLIPF--------- 60

Query: 61  CSLNIAQSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL 120
                        +GT+FLASSM+E+EVNAYRE ERQDANKILD FL LCHQAG  AEKL
Sbjct: 61  -------------LGTQFLASSMKEKEVNAYRERERQDANKILDGFLFLCHQAGVWAEKL 120

Query: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS----------SYSMHTSCTLLC---- 180
           YFESDKIKKGIVELVSLHGIR L+MGAAADKYYS          +  +H      C    
Sbjct: 121 YFESDKIKKGIVELVSLHGIRRLIMGAAADKYYSRKMTKIRSRKAMYVHLKAPAFCQIQF 180

Query: 181 -----IWSTAEDSDEMMEAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMER 240
                +  T E   +  EAH D+ P SPQSQN+NG  WR  + GQFNG    SPSIV+ER
Sbjct: 181 VCNGQLIRTREACPQ--EAHVDVPPPSPQSQNVNGACWRPMESGQFNGSRIQSPSIVLER 240

Query: 241 LTISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTS 300
           L+IS+ +SN SGT SPFERSY+ S SSGC D ASSR+EED YG+GL+SPL L + APN+S
Sbjct: 241 LSISKLVSNDSGTSSPFERSYSPSSSSGCLDAASSRTEEDEYGVGLNSPLLLKDVAPNSS 300

Query: 301 P-HLSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSE 360
           P HLSGFQQD +A+D LYIQLEK+IT+AANA+REAFREA+KRA+AE EL+ +I  AK  E
Sbjct: 301 PLHLSGFQQDESADDILYIQLEKSITEAANAKREAFREAVKRAQAENELENAIFRAKFYE 360

Query: 361 TLYAEESRGRKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVK 420
            LYA E+R RKD EEALSKEREEL+NVKNQVN+MM++LQ++RN  L+LENQIA SD+MVK
Sbjct: 361 DLYAGEARERKDVEEALSKEREELENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVK 420

Query: 421 ELEQKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSE 480
           ELEQKILSAI+LLHNYKNDRDELLKQRDEAL+E+DDIR RQVEAR++ +AQ+ SEFSFSE
Sbjct: 421 ELEQKILSAIELLHNYKNDRDELLKQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSE 480

Query: 481 IVEATRKFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRH 540
           I EATRKF PSLKI EG +GSMYKGLLYNTEV IKM  SHNLQNPVEFQREVD LS LRH
Sbjct: 481 IAEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHNLQNPVEFQREVDVLSILRH 540

Query: 541 PNLATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHS 600
           PN+ATLIGVCPEACILVYDYFPNG+LEDRLACKDNSSPLSWKTRI IA++LCSALIFIHS
Sbjct: 541 PNIATLIGVCPEACILVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHS 600

Query: 601 NKICKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFP 660
           NKICKI+HGD+KP NVLLDANYVPKLAGFG CHFL HDEK SYNEN+S  +    N EFP
Sbjct: 601 NKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENISAKYGVKSNPEFP 660

Query: 661 LTTAFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQ 720
           LTT  DV+SFGMVLLSLLTG+SYLRM+EDVQ AI++RKLKDVLDPRAGDWPFVQAEQLAQ
Sbjct: 661 LTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAIEERKLKDVLDPRAGDWPFVQAEQLAQ 720

Query: 721 LALRCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEV 780
           LALRCCN +S YRPDL+SD WRVLEPM+ASCGGS+SV LSFGD QVQPPPYF+CPIFQEV
Sbjct: 721 LALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEV 780

Query: 781 MEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 819
           MEDPHVAADGFTYEAEALRGWLDSGH+TSPMTNLRL+HQNLVPNRALRSVIQEWL Q Q
Sbjct: 781 MEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQQQ 815

BLAST of MELO3C021208.jh1 vs. ExPASy TrEMBL
Match: A0A6J1L2C9 (E3 ubiquitin ligase OS=Cucurbita maxima OX=3661 GN=LOC111499216 PE=4 SV=1)

HSP 1 Score: 1188 bits (3074), Expect = 0.0
Identity = 623/839 (74.26%), Postives = 696/839 (82.96%), Query Frame = 0

Query: 1   MAGSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISM 60
           MAGS G+D VY+AVGND+NECKLNLVYA+KHSGGRRICILHVHEPAK IP          
Sbjct: 1   MAGSCGDDVVYMAVGNDVNECKLNLVYALKHSGGRRICILHVHEPAKLIPF--------- 60

Query: 61  CSLNIAQSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKL 120
                        +GT+FLASSM+E+EVNAYRE ERQDANKILD FL LCHQAG +AEKL
Sbjct: 61  -------------LGTQFLASSMKEKEVNAYRERERQDANKILDGFLFLCHQAGVRAEKL 120

Query: 121 YFESDKIKKGIVELVSLHGIRSLVMGAAADKYYS----------SYSMHTSCTLLC---- 180
           YFESDKIKKGIVELVSLHGIR L+MGAAADKYYS          +  +H      C    
Sbjct: 121 YFESDKIKKGIVELVSLHGIRRLIMGAAADKYYSRKMTKIRSRKAMYVHLKAPAFCQIQF 180

Query: 181 -----IWSTAEDSDEMMEAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMER 240
                +  T E   +  EAH DI P SPQSQN+NG  WR  + GQFNG    SPSIV+ER
Sbjct: 181 VCNGQLIRTREACPQ--EAHVDIPPPSPQSQNVNGACWRPVEPGQFNGSRIQSPSIVLER 240

Query: 241 LTISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTS 300
           L+IS+ + N SGT SP ERSY+ S SSGC D A SR+EED+YG+GL+SPL L + APN+S
Sbjct: 241 LSISKLVLNDSGTSSPSERSYSPSSSSGCLDAACSRTEEDAYGVGLNSPLLLKDVAPNSS 300

Query: 301 P-HLSGFQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSE 360
           P HL GFQQD +A+D LYIQLEK+IT+AANA+REAFREA+KRA+AE EL  +I  AK  E
Sbjct: 301 PLHLLGFQQDESADDILYIQLEKSITEAANAKREAFREAVKRAQAENELGNAIFRAKFYE 360

Query: 361 TLYAEESRGRKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVK 420
            LYA E+R RKD EEALSKEREE +NVKNQVN+MM++LQ+++N  L+LENQIA SD+MVK
Sbjct: 361 DLYAGEARERKDVEEALSKEREEFENVKNQVNKMMEELQVSQNRGLELENQIAGSDQMVK 420

Query: 421 ELEQKILSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSE 480
           ELEQKILSAI+LLHNYKNDRDELLKQRDEAL+E+DDIR RQVEAR++ +AQ+ SEFSFSE
Sbjct: 421 ELEQKILSAIELLHNYKNDRDELLKQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSE 480

Query: 481 IVEATRKFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRH 540
           I EATRKF PSLKI EG +GSMYKGLLYNTEV IKM  SHNLQNPVEFQREVDALS +RH
Sbjct: 481 IAEATRKFDPSLKIGEGTHGSMYKGLLYNTEVCIKMFGSHNLQNPVEFQREVDALSIMRH 540

Query: 541 PNLATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHS 600
           PN+ATLIGVCPEACILVYDYFPNG+LEDRLACKDNSSPLSWKTRI IA+DLCSALIFIHS
Sbjct: 541 PNIATLIGVCPEACILVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASDLCSALIFIHS 600

Query: 601 NKICKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFP 660
           NKICKI+HGD+KP NVLLDANYVPKLAGFG CH L HDEK SYNEN+S  +    NHEFP
Sbjct: 601 NKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHLLPHDEKLSYNENISAKYGVKSNHEFP 660

Query: 661 LTTAFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQ 720
           LTT  DV+SFGMVLLSLLTG+SYLRM+EDVQ AI++RKLKDVLDPRAGDWPFVQAEQLAQ
Sbjct: 661 LTTKLDVFSFGMVLLSLLTGKSYLRMKEDVQFAIEERKLKDVLDPRAGDWPFVQAEQLAQ 720

Query: 721 LALRCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEV 780
           LALRCCN +S YRPDL+SD WRVLEPM+ASCGGS+SV LSFGD QVQPPPYF+CPIFQEV
Sbjct: 721 LALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEV 780

Query: 781 MEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQHQ 819
           MEDPHVAADGFTYEAEALRGWLDSGH+TSPMTNLRL+HQNLVPNRALRSVIQEWL Q Q
Sbjct: 781 MEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQQQ 815

BLAST of MELO3C021208.jh1 vs. TAIR 10
Match: AT2G45910.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 645.6 bits (1664), Expect = 5.4e-185
Identity = 370/835 (44.31%), Postives = 528/835 (63.23%), Query Frame = 0

Query: 7   EDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISMCSLNIA 66
           ++ ++VAV   + + K  L++A++++GG++IC++HVH+P++ IP                
Sbjct: 44  DEKIFVAVDKHVAKSKSTLIWALQNTGGKKICLIHVHQPSQMIP---------------- 103

Query: 67  QSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKLYFESDK 126
                 ++G KF   +++EEEV  +RE ER+  + ILD +L +C Q G +AEK++ E + 
Sbjct: 104 ------LMGAKFPVGAVKEEEVRVFREKEREKVHMILDDYLRICQQRGVRAEKMFIEMES 163

Query: 127 IKKGIVELVSLHGIRSLVMGAAADKYYSS-----------YSMHTSCTLLCIWSTAE--- 186
           I+ GIV+L+S  GIR LVMGAAAD++YS            +    + TL  IW T +   
Sbjct: 164 IENGIVQLISELGIRKLVMGAAADRHYSRRMTDLKSRKAIFVRREAPTLCQIWFTCKGYL 223

Query: 187 ------DSDEMMEAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLTISE 246
                   D+    +A   PS   S  +   S    +  Q   R+ ++ S       + +
Sbjct: 224 IHTREATMDDTESEYASPRPSISASDLLQTFSTPESEH-QHISRVQSTDS-------VQQ 283

Query: 247 TISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPHLSG 306
            +SN S T    E+S   S  S  TD    R  + S   G ++ +   + +P++ P    
Sbjct: 284 LVSNGSST----EQSGRVSDGSLNTD-EEERESDGSEVTGSATVMSSGHSSPSSFP---- 343

Query: 307 FQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSETLYAEE 366
              DG  +DS  +++ KA ++A ++++EAF E L+R KAEK   ++I  AK SE+ Y+EE
Sbjct: 344 ---DG-VDDSFNVKIRKATSEAHSSKQEAFAETLRRQKAEKNALDAIRRAKQSESAYSEE 403

Query: 367 SRGRKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVKELEQKI 426
            + RKD E A++KE+E    +KN+   +M++LQ A   +  LE+QIA+SD  +++L QK+
Sbjct: 404 LKRRKDTEIAVAKEKERFITIKNEQEVIMEELQSAMAQKAMLESQIAKSDGTMEKLNQKL 463

Query: 427 LSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVEATR 486
             A++LL   +++R+EL  +RD AL+E +++R    E  + +  Q  ++FSFSEI EAT 
Sbjct: 464 DIAVKLLQKLRDEREELQTERDRALREAEELRS-HAETSTLQLPQYFTDFSFSEIEEATN 523

Query: 487 KFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATL 546
            F  +LKI EG  GS+Y GLL +T+V+IKML  ++ Q PVE+Q+EVD LSK+RHPN+ TL
Sbjct: 524 HFDSTLKIGEGGYGSIYVGLLRHTQVAIKMLNPNSSQGPVEYQQEVDVLSKMRHPNIITL 583

Query: 547 IGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKI 606
           IG CPE   LVY+Y P G+LEDRL CKDNS PLSW+ R+ IAT++C+AL+F+HSNK   +
Sbjct: 584 IGACPEGWSLVYEYLPGGSLEDRLTCKDNSPPLSWQNRVRIATEICAALVFLHSNKAHSL 643

Query: 607 VHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENL--SVGH-DAMGNHEFPLTT 666
           VHGD+KP+N+LLD+N V KL+ FG C  L  +   S   ++  +V + D   +    LT 
Sbjct: 644 VHGDLKPANILLDSNLVSKLSDFGTCSLLHPNGSKSVRTDVTGTVAYLDPEASSSGELTP 703

Query: 667 AFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLAL 726
             DVYSFG++LL LLTG+  LR+  +V+ A+    L D+LDP AGDWPFVQAEQLA+LAL
Sbjct: 704 KSDVYSFGIILLRLLTGRPALRISNEVKYALDNGTLNDLLDPLAGDWPFVQAEQLARLAL 763

Query: 727 RCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMED 786
           RCC T S  RPDL ++ WRVLEPMRAS GGS S  L   + ++  PPYFICPIFQEVM+D
Sbjct: 764 RCCETVSENRPDLGTEVWRVLEPMRASSGGSSSFHLGRNEHRI-APPYFICPIFQEVMQD 823

Query: 787 PHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQH 819
           PHVAADGFTYEAEA+R WLDS HDTSPMTN++L H +L+ N ALRS IQEWLQ H
Sbjct: 824 PHVAADGFTYEAEAIRAWLDSEHDTSPMTNVKLSHTSLIANHALRSAIQEWLQHH 833

BLAST of MELO3C021208.jh1 vs. TAIR 10
Match: AT3G49060.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 435.6 bits (1119), Expect = 8.5e-122
Identity = 284/825 (34.42%), Postives = 459/825 (55.64%), Query Frame = 0

Query: 7   EDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISMCSLNIA 66
           ++ ++VAV  D+   K  +++A ++  G++IC+L+VH  A++                 A
Sbjct: 14  DETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARA-----------------A 73

Query: 67  QSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKLYFESDK 126
             ++  +VG      S ++ +V     +E+   +++++ +L L  +   Q +KL      
Sbjct: 74  SWTHKKLVG-----GSFKKHDVKVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQN 133

Query: 127 IKKGIVELVSLHGIRSLVMGAAADKYYS-SYSMHTSCTLLCIWSTAEDSDE---MMEAHA 186
           I++ IVEL++ H I+ LVMGAA+DK+YS   +   S   + +   A DS     + + + 
Sbjct: 134 IEECIVELIARHKIKWLVMGAASDKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYL 193

Query: 187 DISPSSPQSQNINGVSWRTDQFGQFNGRISN---SPSIVMERLTISETISNASG--TWSP 246
             + +S    N         Q    N    +     S +  RL    ++    G      
Sbjct: 194 IFTRASNDDSNNRQTMPPLVQLDSDNETRKSEKLESSYMRRRLRYWRSLLEQDGEKDTGQ 253

Query: 247 FERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPHLSGFQQDGNANDSL 306
            ER      +       SS S  +  G    SP  + +   NTS ++   +++G+    +
Sbjct: 254 LEREKVEPRAPPLFSSGSSSSFGEPVGPEPVSPELVDSDTLNTS-NVEEKEREGDVARKV 313

Query: 307 YIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSETLYAEESRGRKDAEEAL 366
           + + +KA+ D   + R  + EA K+ + +    E++C AK  E L  +ES  RK  EE L
Sbjct: 314 H-RYDKAMHDIGQSDRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKESSQRKRLEELL 373

Query: 367 SKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVKELEQKILSAIQLLHNYK 426
            KE+ E+  V  Q N  MK+LQ+ +   LKLE+Q+ +  ++ KE  +K  +A++LL +++
Sbjct: 374 EKEKLEVKMVIEQNNGFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKFDTAMELLKSFR 433

Query: 427 NDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVEATRKFVPSLKIVEG 486
             RDE+    + A++E++ +R+       + S   + ++SF EI EAT +F PS K+ EG
Sbjct: 434 QKRDEIRIDHENAVKEVNALRRLVKGETGESSGSEMLDYSFMEINEATNEFDPSWKLGEG 493

Query: 487 ENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATLIGVCPEACILV 546
           + GS+YKG L + +V++KML S+   N  EF+R V+ LS++RHPNL TL+G CPE+  L+
Sbjct: 494 KYGSVYKGNLQHLQVAVKMLPSYGSLNHFEFERRVEILSRVRHPNLVTLMGACPESRSLI 553

Query: 547 YDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKIVHGDMKPSNVL 606
           Y Y PNG+LED  + ++N   LSW++RI IA+++CSAL+F+HSN  C I+HG++KPS +L
Sbjct: 554 YQYIPNGSLEDCFSSENNVPALSWESRIRIASEICSALLFLHSNIPC-IIHGNLKPSKIL 613

Query: 607 LDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEF---PLTTAFDVYSFGMVL 666
           LD+N V K+  +GI   +  D        L      +  H F    +T   D+Y+FG++L
Sbjct: 614 LDSNLVTKINDYGISQLIPID-------GLDKSDPHVDPHYFVSREMTLESDIYAFGIIL 673

Query: 667 LSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCNTDSTYRP 726
           L LLT +    +  DV+ A++   +  VLD  AGDWP  + ++LA +A+RCC  +   RP
Sbjct: 674 LQLLTRRPVSGILRDVKCALENDNISAVLDNSAGDWPVARGKKLANVAIRCCKKNPMNRP 733

Query: 727 DLMSDAWRVLEPMRASCGGSLSVCLSFGDQQV--QPPPYFICPIFQEVMEDPHVAADGFT 786
           DL +   R ++ M+A    S S   S+ +Q V  +PP +++CPIFQEVM+DP +AADGFT
Sbjct: 734 DL-AVVLRFIDRMKAPEVPS-SETSSYANQNVPRRPPSHYLCPIFQEVMKDPLIAADGFT 793

Query: 787 YEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQ 818
           YEAEA+R WL +GHDTSPMTNL+++  NL+PN AL   IQ+W  Q
Sbjct: 794 YEAEAIREWLANGHDTSPMTNLKMEDCNLIPNHALHLAIQDWQNQ 804

BLAST of MELO3C021208.jh1 vs. TAIR 10
Match: AT3G49060.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 412.1 bits (1058), Expect = 1.0e-114
Identity = 274/836 (32.78%), Postives = 449/836 (53.71%), Query Frame = 0

Query: 7   EDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKSIPICKARKEISMCSLNIA 66
           ++ ++VAV  D+   K  +++A ++  G++IC+L+VH  A++                 A
Sbjct: 14  DETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARA-----------------A 73

Query: 67  QSSNDFIVGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKLYFESDK 126
             ++  +VG      S ++ +V     +E+   +++++ +L L  +   Q +KL      
Sbjct: 74  SWTHKKLVG-----GSFKKHDVKVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQN 133

Query: 127 IKKGIVELVSLHGIRSLVMGAAADKYYS--------------------SYSMHTSCTLLC 186
           I++ IVEL++ H I+ LVMGAA+DK+YS                    S  +   C    
Sbjct: 134 IEECIVELIARHKIKWLVMGAASDKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYL 193

Query: 187 IWSTAEDSDEMMEAHADISPSSPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLTISE 246
           I++ A + D     +    P   Q  + N           +  R       ++E++ + +
Sbjct: 194 IFTRASNDD---SNNRQTMPPLVQLDSDNETRKSEKLESSYMRRRLRYWRSLLEQVNLLQ 253

Query: 247 TISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPHLSG 306
             ++        ER      +       SS S  +  G    SP  + +   NTS ++  
Sbjct: 254 NCADGEKDTGQLEREKVEPRAPPLFSSGSSSSFGEPVGPEPVSPELVDSDTLNTS-NVEE 313

Query: 307 FQQDGNANDSLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSETLYAEE 366
            +++G+    ++ + +KA+ D   + R  + EA K+ + +    E++C AK  E L  +E
Sbjct: 314 KEREGDVARKVH-RYDKAMHDIGQSDRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKE 373

Query: 367 SRGRKDAEEALSKEREELDNVKNQVNEMMKDLQIARNNELKLENQIAQSDEMVKELEQKI 426
           S  RK  EE L KE+ E+  V  Q N  MK+LQ+ +   LKLE+Q+ +  ++ KE  +K 
Sbjct: 374 SSQRKRLEELLEKEKLEVKMVIEQNNGFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKF 433

Query: 427 LSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVEATR 486
            +A++LL +++  RDE+    + A++E++ +R+       + S   + ++SF EI EAT 
Sbjct: 434 DTAMELLKSFRQKRDEIRIDHENAVKEVNALRRLVKGETGESSGSEMLDYSFMEINEATN 493

Query: 487 KFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATL 546
           +F PS K+ EG+ GS+YKG L + +V+                  V+ LS++RHPNL TL
Sbjct: 494 EFDPSWKLGEGKYGSVYKGNLQHLQVA------------------VEILSRVRHPNLVTL 553

Query: 547 IGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKI 606
           +G CPE+  L+Y Y PNG+LED  + ++N   LSW++RI IA+++CSAL+F+HSN  C I
Sbjct: 554 MGACPESRSLIYQYIPNGSLEDCFSSENNVPALSWESRIRIASEICSALLFLHSNIPC-I 613

Query: 607 VHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEF---PLTT 666
           +HG++KPS +LLD+N V K+  +GI   +  D        L      +  H F    +T 
Sbjct: 614 IHGNLKPSKILLDSNLVTKINDYGISQLIPID-------GLDKSDPHVDPHYFVSREMTL 673

Query: 667 AFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLAL 726
             D+Y+FG++LL LLT +    +  DV+ A++   +  VLD  AGDWP  + ++LA +A+
Sbjct: 674 ESDIYAFGIILLQLLTRRPVSGILRDVKCALENDNISAVLDNSAGDWPVARGKKLANVAI 733

Query: 727 RCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGDQQV--QPPPYFICPIFQEVM 786
           RCC  +   RPDL +   R ++ M+A    S S   S+ +Q V  +PP +++CPIFQEVM
Sbjct: 734 RCCKKNPMNRPDL-AVVLRFIDRMKAPEVPS-SETSSYANQNVPRRPPSHYLCPIFQEVM 793

Query: 787 EDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQ 818
           +DP +AADGFTYEAEA+R WL +GHDTSPMTNL+++  NL+PN AL   IQ+W  Q
Sbjct: 794 KDPLIAADGFTYEAEAIREWLANGHDTSPMTNLKMEDCNLIPNHALHLAIQDWQNQ 794

BLAST of MELO3C021208.jh1 vs. TAIR 10
Match: AT5G51270.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 272.3 bits (695), Expect = 1.3e-72
Identity = 231/782 (29.54%), Postives = 371/782 (47.44%), Query Frame = 0

Query: 85  EEEVNAYRELERQDANK-ILDVFLLLCHQAGAQAEKLYFESDKIKKGIVELVSLHGIRSL 144
           EE   AYR+   Q+  + +L  F  +C +   + ++  FES   K      V L  + S 
Sbjct: 90  EEVAAAYRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVA----VELQVLESN 149

Query: 145 VMGAAADKYYSSYSMHTSCTLLCIWSTAEDSDEMMEAHADISPSSPQSQNINGVSWRTDQ 204
            +  A  K     + H    L+   S+   S    +  A IS S      +  VS     
Sbjct: 150 SVAVAITK---EVNQHLISNLIIGRSSQAASSRNYDITASISASVSNLCTVYVVS----- 209

Query: 205 FGQFNGRISNSPSIVMERLTISETISNASGTWSPFERSYATSPSSGCTDVASSRSEEDSY 264
               NG +      ++ + T S+T  N +   S FER+     SS C+  + + S+  S 
Sbjct: 210 ----NGGVH-----ILAKDT-SDTERNDTSIESGFERT-----SSSCSSGSGANSDVMSN 269

Query: 265 GLGLSSPLRLTNFAPNTSPHLSGFQQDGNANDSLYIQLEKAITDAANARR---EAFREAL 324
            L  S+P  L+N      P +            + + +E + T++   ++   +A  EA 
Sbjct: 270 AL-KSNPHTLSNKRMQNLPTIV---------RGVSVPMETSSTESDETKKRSSDAAEEAS 329

Query: 325 KRAKAEKELDESICWAKVSETLYAEESRGRKDAEEALSKEREELDNVKNQVNEMMKDLQI 384
           KR  +  E   S+ W          +   RKDA  ++S    E  NV   +     D Q 
Sbjct: 330 KR--SSPETSRSVSW-----NPQFRDFDERKDAMSSMS-SNFEYGNVVTPLGHYFTDNQD 389

Query: 385 ARNNELKLENQIAQSDEMVKELEQKILSAIQLLHNYKNDRDELLKQRDEAL-----QELD 444
             N   KL  ++  + EM    + + L A + L+  K +   LL+   + +     ++ +
Sbjct: 390 TLNEISKLRAELRHAHEMYAVAQVETLDASRKLNELKFEELTLLEHETKGIAKKETEKFE 449

Query: 445 DIRKRQVEARSQRSAQL-----------------------ISEFSFSEIVEATRKFVPSL 504
             R+ + EA  +R A++                         EF++ EI+ AT  F   L
Sbjct: 450 QKRREEREAAQRREAEMKATHEAKEKEKLEESSLVAPKLQYQEFTWEEIINATSSFSEDL 509

Query: 505 KIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATLIGVCPE 564
           KI  G  G +YK  L++T  ++K+L S       +F +E++ LSK+RHP+L  L+G CP+
Sbjct: 510 KIGMGAYGDVYKCNLHHTIAAVKVLHSAESSLSKQFDQELEILSKIRHPHLVLLLGACPD 569

Query: 565 ACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKIVHGDMK 624
              LVY+Y  NG+LEDRL   ++S P+ W  R+ IA ++ SAL+F+H +K   I+H D+K
Sbjct: 570 HGALVYEYMENGSLEDRLFQVNDSQPIPWFVRLRIAWEVASALVFLHKSKPTPIIHRDLK 629

Query: 625 PSNVLLDANYVPKLAGFGICHFLTHDEKSS-----YNENLSVGHDAMGNHEFPLTTAF-- 684
           P+N+LL+ N+V K+   G+   +   +  S     Y +   VG     + E+  T     
Sbjct: 630 PANILLNHNFVSKVGDVGLSTMIQAADPLSTKFTMYKQTSPVGTLCYIDPEYQRTGRISP 689

Query: 685 --DVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKR---KLKDVLDPRAGDWPFVQAEQLAQ 744
             DVY+FGM++L LLTGQ  + +   V+ A++     +L  +LD +AG+WP  +  QLA 
Sbjct: 690 KSDVYAFGMIILQLLTGQQAMALTYTVETAMENNNDDELIQILDEKAGNWPIEETRQLAA 749

Query: 745 LALRCCNTDSTYRPDLMSDAWRVLEPMRA---SCGGSLSVCLSFGDQQVQPPPYFICPIF 804
           LAL+C    S  RPDL      VLE ++        SLS   S      QPP +F CP+ 
Sbjct: 750 LALQCTELRSKDRPDLEDQILPVLESLKKVADKARNSLSAAPS------QPPSHFFCPLL 809

Query: 805 QEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQ 820
           ++VM++P +AADG+TY+  A+  W+++ H TSP+TN  L + NL+PN  L + I EW  +
Sbjct: 810 KDVMKEPCIAADGYTYDRRAIEEWMEN-HRTSPVTNSPLQNVNLLPNHTLYAAIVEWRNR 819

BLAST of MELO3C021208.jh1 vs. TAIR 10
Match: AT4G25160.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 266.9 bits (681), Expect = 5.3e-71
Identity = 231/791 (29.20%), Postives = 366/791 (46.27%), Query Frame = 0

Query: 74  VGTKFLASSMEEEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKLYFESDKIKKGIVE 133
           +G     S + ++ V AYR+     + ++L  +  L  +     E L  ESD +   I E
Sbjct: 67  MGNAIPISEVRDDVVTAYRQEILWQSEEMLKPYTKLFVRRKVAVEVLVIESDNVAAAIAE 126

Query: 134 LVSLHGIRSLVMGAAADKYYS---------SYSMHTSCT--------LLCIWSTAEDSDE 193
            V+   I  +V+G ++  ++S         S  M   CT        L C+     DSD 
Sbjct: 127 EVTRDSIDRIVIGGSSRSFFSRKADICSVISALMPNFCTVYVVSKGKLSCV--RPSDSDG 186

Query: 194 MMEAHADISPSSPQSQNING-VSWRTDQFGQFNGRISNSPSIVMERLTISETISNASGTW 253
                 D S  +  S   +G  S  +D     +   S   S+ + R+     I+  +   
Sbjct: 187 NATIREDGSERTNSSSGSSGPTSDSSDVMSSAHDSQSRPLSLPVRRMQHFPAIAGQASV- 246

Query: 254 SPFERSYATSPSSGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPHLSGFQQDGNAND 313
            P E S   S  + C  + +  + + S  +  SS    T+     +P    +++   A  
Sbjct: 247 -PMETSSVGSDETRCMSLDAEEARDVS-SINRSS----TDTTSRWTPRRRDYEERKEAMS 306

Query: 314 SLYIQLEKAITDAANARREAFREALKRAKAEKELDESICWAKVSETLYAEESRGRKDAEE 373
           S           ++N     F      +    +   S    + S    A   +   D + 
Sbjct: 307 S----------SSSNREYGNFGTRFSWSGMGVDTTHSRASQQASNMSDALSEQSYTDNQV 366

Query: 374 ALSKEREELDNVKNQVNEMMKDLQ---------IARNNELKLENQIAQSDEMVKELEQKI 433
            L+ E E+L      V EM    Q         +   N+ +LE  I   +  +KE E + 
Sbjct: 367 NLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGELNQRRLEEAIKLEELKLKEYEARE 426

Query: 434 LSAIQLLHNYKNDRDELLKQRDEALQELDDIRKRQVEARSQR-------------SAQL- 493
           L A +   N++  R +    R+ A +E+   ++R+ E +S R             S QL 
Sbjct: 427 L-AEKEKQNFEKARRDAESMRERAEREI--AQRREAERKSARDTKEKEKLEGTLGSPQLQ 486

Query: 494 ISEFSFSEIVEATRKFVPSLKIVEGENGSMYKGLLYNTEVSIKMLCSHNLQNPVEFQREV 553
              F++ EI+ AT  F   LKI  G  G++YK  L++T   +K+L S   Q   +FQ+E+
Sbjct: 487 YQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTTAVVKVLQSAENQLSKQFQQEL 546

Query: 554 DALSKLRHPNLATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLC 613
           + LSK+RHP+L  L+G CPE   LVY+Y  NG+LEDRL   +NS PL W  R  IA ++ 
Sbjct: 547 EILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQVNNSPPLPWFERFRIAWEVA 606

Query: 614 SALIFIHSNKICKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSS----YNENLS 673
           +AL+F+H +K   I+H D+KP+N+LLD N+V K+   G+   +  D  S+    Y +   
Sbjct: 607 AALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTSP 666

Query: 674 VGHDAMGNHEFP----LTTAFDVYSFGMVLLSLLTGQSYLRMEEDVQIAIKKR-KLKDVL 733
           VG     + E+     +++  D+YSFGM+LL LLT +  + +   V+ A+    +   +L
Sbjct: 667 VGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKPAIALTHFVESAMDSNDEFLKIL 726

Query: 734 DPRAGDWPFVQAEQLAQLALRCCNTDSTYRPDLMSDAWRVLEPMRASCGGSLSVCLSFGD 793
           D +AG+WP  +  +LA LAL C       RPDL       LE ++     + +   SF  
Sbjct: 727 DQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILPALENLKKVAEKARN---SFSG 786

Query: 794 QQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVP 815
              QPP +FICP+ ++VM +P VAADG+TY+  A+  WL   H+TSPMT+  L  +NL+P
Sbjct: 787 VSTQPPTHFICPLLKDVMNEPCVAADGYTYDRHAIEEWLKE-HNTSPMTDSPLHSKNLLP 831