MELO3C021113 (gene) Melon (DHL92) v4

Overview
NameMELO3C021113
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionAsparagine--tRNA ligase
Locationchr01: 26620204 .. 26625107 (+)
RNA-Seq ExpressionMELO3C021113
SyntenyMELO3C021113
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAGTTAGTTAAGACCAAAGTTTGAAGTTTAGATGGAGAGTGTTTTTTCCAAAGTTATATAATATAATAATTCATTTTTATTCCAACAGTTTGTAAACAGGTCGCTTTCAAGTAAGGTTTCAATTTCCCATCAAAGAAAACAAACTCTCAAATGAACAAAAAACAAACTCTTCAAGAAAATCTAAACTTCAAAGTATTCCAAAACCTCAAACTCTCTTTAAAATCTCTCTCAGTTCATACCACAAAATCTCCCCAAATCATTTCTCTGAAACAATCCTTTTCCCTCCATTAACAATCTCTCTTGATTATTTCCCCCTCTTCTTCCCCAAGAAAAGCATCTCAATCTTCTTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTTATATCTCTCAATCCATGTCTCTTCTCCTTCATCATTCAATCCCCCCATTTCCCTCTTCTTTCTTCCTCCACACTCTTCCATGGCCGCCCAACAACCTACAGTCTTCTCCCCCATCACCTTCTCCAAATACTCCAACAGGGTTCTTCTCAAAACCCTCTTGGATACCACCGATGGGGGTCTTTCGTTTGTGGGTCAGAAACTTGTGGTCGGAGGATGGGTCAAGTCCTCCAAGGAAGTTATTCGAGATGCTCCCCCGGCTCCGGCTCCGGCTGATTCTCTGCCTCCTAAGCTTAATAAGGATGTGAGCTGTATTGAGATTATTCAATCGCGGATTCCGATTGTTAGGTCTTTGCTTAAGATGTTGTATGGAAATAACTTGCACGGTGGGGAGAAGTTGGACACTCCTGTTCATAAGCCGCCTCTTCCTTCCACTGCTTTTCTGGCTATTAATGATGGGTCTTGTGTTGCAAGCCTTCAGGTACCTCTTTTTTGCTACGCACGCCAACTGTTTGCTACTTTGTTTTGCGTATAGGATGTGGCTAACTTAAAAGTTTGGCGTATTTCACTATCAAAACTTATTATTTGTATACAATGGTACTACTTCTTTTGTCTTTTGTCTTTTTGTTGAGAAACTATTCTGTGATCCTGCCACAGTTTTCCATCACTGTATATTCTATGACATATCTAATGGTTGTTAGAGGCATACAAATTTTGAATTCACGTCCAATGGTTCTTGCGAGACAGCTACACTACTGCATAATTTGACCTTTTTATGATTAAAATGACTTTTAATGTTTTTCCCCTGTATCTGTTTTAAAATTTTTTATTGTGTTGCATATGGATGAATTTGTATGTTCTTCCCTATCCAAAAAAGAAATATGGAACTTTTAAATGAATTTAAAGTTGATGCAGAGTTAAGGAAGGTCTGATATTTTTTATTACAATGTGTTAGAGGATAATTGGTTGCTTAAGCTTTGGTTGTGCTTGGTTTTTTGCATTCTCACAGTAATAAGAAACTTGTAGGTTGTCGTTGAGTCCTCATTACATCATCCCACCCATCTGCTGCCAACGGGAACTTGCATCTTGGTGGAAGGCGTACTGAAGCAGGTCCCGGTACCTGGGAAACATGTTGTTCGGCTTGAAGTCGAGAAAATTCTTCATGTAGGAAAGGTTGAACAGGAAAAGTATCCATTATCCCAGAAAAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACAACTACGGTAATATGAATAAGCTTCAACTATAAGTTCCATTTATTTCCTATTTTTATGGTTCTGTTTGCATGCCTATAATTAAAGTGCCTATTAGCTTTAAGATCGTGCTTACTGCTTATGCAACAGCACCATGTGAATTCAAAGCGTTCTGTTCATATTTTATATCTAGTGATTGGTAAAACGGAGCCTTGAGATTCGAAAACAGGAGATTTATACAGTTAAGAGCTCATTTTCTGCTAAAAGAAAGAATTGATATATATAGTTCAACGTTCCTGATCATGGATTAAAGTAACTTCATTGTAAATCTCCTTGGTTGGATCGCTTGCATTGACCTTTATTGAACCTGTGCAATGGAATTTGGTGTCAAAGCTTGAGAAGAGAGTCTTGCATATCTAATTATTAGAACTAATGAAGTGTTGGATCTTGATCTTCACTGGTATTCTTTACTTTATGCAGGTTGCATCTGTTACGAGAATCCGTAGTGCCTTGACTTTTGCAAATCACACGTTCTTCCAAAATAACAGCTTTCTTTACGTTCAATTACCCACCATCACAACCACAGACAGTGAAGGATTCAGCGAAAAGTTCCACGTTACAACACTTGCAGGTAAAGGTAAGAGAGTGGAATCAACAGAAGACAAAGAACCCGATGGTTTAAGTCTTGAAACTGTTAAGGCTGCTATAACGGAGAAAAGCAATCTTGTCAAAGAGCTTGAGAGAAGCGAAAGCAACAGAGAAGCCTTAGTTGCTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAAGCCAGAGAAAAATCAAAATCCAAATCGAGGAAAGATAAAGTTATTCCTGGAAATTTTTTACCACAAGAAACTTATCTAACGGTTTCTGGACAACTGCATTTGGAGACTTATGCATGTGCCCTTGGCAATGTGTACTCGTGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGAACTGAAGGTATGAATTTGATCTTTTACTTATTTCTTAAGATAGCTACTTTATATTATATGGGTTTAAGATTCCGTGTTGATTTAGCCATTAAAATCCAATGTTATTTTCTGTGGTTAATACTTTATCATAATAACTTTGCACCATTGAAATGAAGATTTTATATCGTTTAACTTGGATTTTTGTATCATCATTTGGAGTCCAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTGTTGGAACACTGTATGGACGATATGAAATTTGTTCAAAAACGCATTGACAAGACTAGCATCCATCGCCTTGAATCCATTATATCATGTTCATTTGAGAAGGTCTCTTACACTGCCGCAATTGAAATCCTGGAAAAGGTGAAATCTAGATTATCTACTCTTCTATCATGTGACTCAGTTTTTCATTTCTACCCCTGCCCATCTGAATGTTGAGAAAAAAAAAATCTTCACTGTCAGGCCACCGATCAAAAATCTCAACACTTAAAAAAGCTTAAATTTGGTGCTGCTTTAACTGCTGATCATCTCAGGTGTGCAAAATGTGCTATTACAGTCGGTCACTATGCTATTTTTCGTTACGAATAAAAAAGTTCTTTCTTCTTTGTACGTTAAACAGTTATCTGGCGGATACCATCTTTAAAAAGCCTGTAATAGTATATAACTACCCGAAAGAAACCAAGCCATTTAATGTCCGCCTGAATGATGATGGGAAAACCGTGGCAGCATTTGATGTGGTTCTTCCAAAGGTTAGCACATGGGCATGTATTTACCCGTGGGAAAATTTATTTTTAGATGGATCCTGAATTGAATGAAAAATTATTTCTGAAAACTGGGTCCCAAATGTTTTGCAGGGTGGAGTGGTAATTTCTGGCAGCCAAAAGGAGGAGCGTCTTGATGTATTGATGACAAGGTATAATATTTTACTGCTTTGATTACTATTCTGTTGATATTTTTCTTGTCTATTCCATCCAAGACTTCCAGTCTTCAAACTTCCAAATCTTCTAAAATTGTTTTTTAATAAATAGACATGACATTGTCTGTCACTTCTTTTACTTATTTACTAGATCAGTAGATATCTACATCGGTTGGATGTTCGGGGATTCCATTATAGATTTTGGATTTTTTAAACAATTAATGAAATAAAATGGGAAGGTTTAAGATCAAGTGGAAAGATGAAATATTTTGAAGTTGAGCGATCAAAATCAAAAGAAATTCTAACTTTCATGAGTTATTTTAAAAAAGAATACCAAGATTTACATTGAGGGAATGATAAAAAAGTAACGTTAGATAGGAAAGACTATAAGCAAAAATAAAAACATCAAAACTTAGAGGGATAAAAGTCCTAAAACTTGACAAAAACAAACATTTCAAAGTTTGCTAGCCCAAGCGTGTAATTAGCATGAGTTTTCATCCTAAAGGTAAGAAATTTCATCTCCATCCCCACAACTAAAAAAAAAAAAAAGAGAGACTATAAATGCATTCCAGCTGCAATCTATGTTCTTGAATTGAAAAGATTTAGTTTAACTACCATGAGTTCAAACCCAGCGATAGTTGCTTGAGATAAAATCTTACTAGTTTGACGACACTACGCTCACAGATAATAGAAAGAAAAAATGTGAGAGTTATTGATATTTGTTTGAATATGGATGGTTGTTGGCAGGATGAAGGAGATGGGTCTGCCAAGGGAGCAATACGAGTGGTACTTAGAGATTCAAAGGCATGGAACAGTGAAGCACTCTGGTTTTAGTTTGGCATTCGATCGTTTCGTTCTGTTTACTACTGGCCTCACTGACATCAAGGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGTCATCAATTAAAGAGAATCCAAGGATAAAATTGTTCAAAAATGTTTTACAACGTAGCTGGGCCCCGACCAATGGTGATGCTTAAAAATTGGAACTGAAAACTGTGTTATCGTAAAAACTAAAAAGTCAAAGGGAAAATGAATTCTGTTGGTCGACTTGTGTTTGTTTGTTGCTAAGATTTGTTCTTATAAATAAAAGAGAATTTGATATCAAATGGATTATTTGTGGAGGAGACATGATTCAAACTTGTGATCTCGAGGTCGTGTATATTTTCTTAATTGCTATGCTTAGCTTCAATCATTATGATTTCTTATTCAAATCTTATGCAAATAATTCTGTTCTGAATGCTTTCATTACATTAAATAGTGAAAAG

mRNA sequence

AAAAAGTTAGTTAAGACCAAAGTTTGAAGTTTAGATGGAGAGTGTTTTTTCCAAAGTTATATAATATAATAATTCATTTTTATTCCAACAGTTTGTAAACAGGTCGCTTTCAAGTAAGGTTTCAATTTCCCATCAAAGAAAACAAACTCTCAAATGAACAAAAAACAAACTCTTCAAGAAAATCTAAACTTCAAAGTATTCCAAAACCTCAAACTCTCTTTAAAATCTCTCTCAGTTCATACCACAAAATCTCCCCAAATCATTTCTCTGAAACAATCCTTTTCCCTCCATTAACAATCTCTCTTGATTATTTCCCCCTCTTCTTCCCCAAGAAAAGCATCTCAATCTTCTTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTTATATCTCTCAATCCATGTCTCTTCTCCTTCATCATTCAATCCCCCCATTTCCCTCTTCTTTCTTCCTCCACACTCTTCCATGGCCGCCCAACAACCTACAGTCTTCTCCCCCATCACCTTCTCCAAATACTCCAACAGGGTTCTTCTCAAAACCCTCTTGGATACCACCGATGGGGGTCTTTCGTTTGTGGGTCAGAAACTTGTGGTCGGAGGATGGGTCAAGTCCTCCAAGGAAGTTATTCGAGATGCTCCCCCGGCTCCGGCTCCGGCTGATTCTCTGCCTCCTAAGCTTAATAAGGATGTGAGCTGTATTGAGATTATTCAATCGCGGATTCCGATTGTTAGGTCTTTGCTTAAGATGTTGTATGGAAATAACTTGCACGGTGGGGAGAAGTTGGACACTCCTGTTCATAAGCCGCCTCTTCCTTCCACTGCTTTTCTGGCTATTAATGATGGGTCTTGTGTTGCAAGCCTTCAGGTTGTCGTTGAGTCCTCATTACATCATCCCACCCATCTGCTGCCAACGGGAACTTGCATCTTGGTGGAAGGCGTACTGAAGCAGGTCCCGGTACCTGGGAAACATGTTGTTCGGCTTGAAGTCGAGAAAATTCTTCATGTAGGAAAGGTTGAACAGGAAAAGTATCCATTATCCCAGAAAAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACAACTACGGTTGCATCTGTTACGAGAATCCGTAGTGCCTTGACTTTTGCAAATCACACGTTCTTCCAAAATAACAGCTTTCTTTACGTTCAATTACCCACCATCACAACCACAGACAGTGAAGGATTCAGCGAAAAGTTCCACGTTACAACACTTGCAGGTAAAGGTAAGAGAGTGGAATCAACAGAAGACAAAGAACCCGATGGTTTAAGTCTTGAAACTGTTAAGGCTGCTATAACGGAGAAAAGCAATCTTGTCAAAGAGCTTGAGAGAAGCGAAAGCAACAGAGAAGCCTTAGTTGCTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAAGCCAGAGAAAAATCAAAATCCAAATCGAGGAAAGATAAAGTTATTCCTGGAAATTTTTTACCACAAGAAACTTATCTAACGGTTTCTGGACAACTGCATTTGGAGACTTATGCATGTGCCCTTGGCAATGTGTACTCGTGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGAACTGAAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTGTTGGAACACTGTATGGACGATATGAAATTTGTTCAAAAACGCATTGACAAGACTAGCATCCATCGCCTTGAATCCATTATATCATGTTCATTTGAGAAGGTCTCTTACACTGCCGCAATTGAAATCCTGGAAAAGGCCACCGATCAAAAATCTCAACACTTAAAAAAGCTTAAATTTGGTGCTGCTTTAACTGCTGATCATCTCAGTTATCTGGCGGATACCATCTTTAAAAAGCCTGTAATAGTATATAACTACCCGAAAGAAACCAAGCCATTTAATGTCCGCCTGAATGATGATGGGAAAACCGTGGCAGCATTTGATGTGGTTCTTCCAAAGGGTGGAGTGGTAATTTCTGGCAGCCAAAAGGAGGAGCGTCTTGATGTATTGATGACAAGGATGAAGGAGATGGGTCTGCCAAGGGAGCAATACGAGTGGTACTTAGAGATTCAAAGGCATGGAACAGTGAAGCACTCTGGTTTTAGTTTGGCATTCGATCGTTTCGTTCTGTTTACTACTGGCCTCACTGACATCAAGGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGTCATCAATTAAAGAGAATCCAAGGATAAAATTGTTCAAAAATGTTTTACAACGTAGCTGGGCCCCGACCAATGGTGATGCTTAAAAATTGGAACTGAAAACTGTGTTATCGTAAAAACTAAAAAGTCAAAGGGAAAATGAATTCTGTTGGTCGACTTGTGTTTGTTTGTTGCTAAGATTTGTTCTTATAAATAAAAGAGAATTTGATATCAAATGGATTATTTGTGGAGGAGACATGATTCAAACTTGTGATCTCGAGGTCGTGTATATTTTCTTAATTGCTATGCTTAGCTTCAATCATTATGATTTCTTATTCAAATCTTATGCAAATAATTCTGTTCTGAATGCTTTCATTACATTAAATAGTGAAAAG

Coding sequence (CDS)

ATGGCCGCCCAACAACCTACAGTCTTCTCCCCCATCACCTTCTCCAAATACTCCAACAGGGTTCTTCTCAAAACCCTCTTGGATACCACCGATGGGGGTCTTTCGTTTGTGGGTCAGAAACTTGTGGTCGGAGGATGGGTCAAGTCCTCCAAGGAAGTTATTCGAGATGCTCCCCCGGCTCCGGCTCCGGCTGATTCTCTGCCTCCTAAGCTTAATAAGGATGTGAGCTGTATTGAGATTATTCAATCGCGGATTCCGATTGTTAGGTCTTTGCTTAAGATGTTGTATGGAAATAACTTGCACGGTGGGGAGAAGTTGGACACTCCTGTTCATAAGCCGCCTCTTCCTTCCACTGCTTTTCTGGCTATTAATGATGGGTCTTGTGTTGCAAGCCTTCAGGTTGTCGTTGAGTCCTCATTACATCATCCCACCCATCTGCTGCCAACGGGAACTTGCATCTTGGTGGAAGGCGTACTGAAGCAGGTCCCGGTACCTGGGAAACATGTTGTTCGGCTTGAAGTCGAGAAAATTCTTCATGTAGGAAAGGTTGAACAGGAAAAGTATCCATTATCCCAGAAAAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACAACTACGGTTGCATCTGTTACGAGAATCCGTAGTGCCTTGACTTTTGCAAATCACACGTTCTTCCAAAATAACAGCTTTCTTTACGTTCAATTACCCACCATCACAACCACAGACAGTGAAGGATTCAGCGAAAAGTTCCACGTTACAACACTTGCAGGTAAAGGTAAGAGAGTGGAATCAACAGAAGACAAAGAACCCGATGGTTTAAGTCTTGAAACTGTTAAGGCTGCTATAACGGAGAAAAGCAATCTTGTCAAAGAGCTTGAGAGAAGCGAAAGCAACAGAGAAGCCTTAGTTGCTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAAGCCAGAGAAAAATCAAAATCCAAATCGAGGAAAGATAAAGTTATTCCTGGAAATTTTTTACCACAAGAAACTTATCTAACGGTTTCTGGACAACTGCATTTGGAGACTTATGCATGTGCCCTTGGCAATGTGTACTCGTGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGAACTGAAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTGTTGGAACACTGTATGGACGATATGAAATTTGTTCAAAAACGCATTGACAAGACTAGCATCCATCGCCTTGAATCCATTATATCATGTTCATTTGAGAAGGTCTCTTACACTGCCGCAATTGAAATCCTGGAAAAGGCCACCGATCAAAAATCTCAACACTTAAAAAAGCTTAAATTTGGTGCTGCTTTAACTGCTGATCATCTCAGTTATCTGGCGGATACCATCTTTAAAAAGCCTGTAATAGTATATAACTACCCGAAAGAAACCAAGCCATTTAATGTCCGCCTGAATGATGATGGGAAAACCGTGGCAGCATTTGATGTGGTTCTTCCAAAGGGTGGAGTGGTAATTTCTGGCAGCCAAAAGGAGGAGCGTCTTGATGTATTGATGACAAGGATGAAGGAGATGGGTCTGCCAAGGGAGCAATACGAGTGGTACTTAGAGATTCAAAGGCATGGAACAGTGAAGCACTCTGGTTTTAGTTTGGCATTCGATCGTTTCGTTCTGTTTACTACTGGCCTCACTGACATCAAGGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGTCATCAATTAA

Protein sequence

MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVIN
Homology
BLAST of MELO3C021113 vs. NCBI nr
Match: XP_008458080.1 (PREDICTED: asparagine--tRNA ligase, cytoplasmic 2 [Cucumis melo])

HSP 1 Score: 1204.5 bits (3115), Expect = 0.0e+00
Identity = 613/613 (100.00%), Postives = 613/613 (100.00%), Query Frame = 0

Query: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60
           MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAF 120
           PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAF
Sbjct: 61  PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAF 120

Query: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180
           LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV
Sbjct: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180

Query: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240
           GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ
Sbjct: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240

Query: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELER 300
           LPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELER
Sbjct: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELER 300

Query: 301 SESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHL 360
           SESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHL
Sbjct: 301 SESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHL 360

Query: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420
           ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW
Sbjct: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420

Query: 421 VLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKF 480
           VLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKF
Sbjct: 421 VLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKF 480

Query: 481 GAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540
           GAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG
Sbjct: 481 GAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540

Query: 541 SQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKD 600
           SQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKD
Sbjct: 541 SQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKD 600

Query: 601 VIPFPRSFGKVIN 614
           VIPFPRSFGKVIN
Sbjct: 601 VIPFPRSFGKVIN 613

BLAST of MELO3C021113 vs. NCBI nr
Match: TYJ96325.1 (asparagine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 612/617 (99.19%), Postives = 613/617 (99.35%), Query Frame = 0

Query: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAP-- 60
           MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAP  
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  --PAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120
             PAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP
Sbjct: 61  PAPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120

Query: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
           STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180

Query: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
           ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240

Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK 300
           LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK 300

Query: 301 ELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG 360
           ELERSESNREALVAAVQDLKKTN+LALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG
Sbjct: 301 ELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG 360

Query: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420
           QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF
Sbjct: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420

Query: 421 LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLK 480
           LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLK
Sbjct: 421 LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLK 480

Query: 481 KLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540
           KLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV
Sbjct: 481 KLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540

Query: 541 VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT 600
           VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT
Sbjct: 541 VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT 600

Query: 601 DIKDVIPFPRSFGKVIN 614
           DIKDVIPFPRSFGKVIN
Sbjct: 601 DIKDVIPFPRSFGKVIN 617

BLAST of MELO3C021113 vs. NCBI nr
Match: XP_004149331.1 (asparagine--tRNA ligase, cytoplasmic 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 595/613 (97.06%), Postives = 601/613 (98.04%), Query Frame = 0

Query: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60
           MAAQQPTVF+PITFSKYSNRVLLKTLLDTTDGGLSFVGQK VVGGWVKSSKEVIRDAPPA
Sbjct: 1   MAAQQPTVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60

Query: 61  PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAF 120
           PAPADSLPPK+NKDVSCIEIIQSRIPIVRSLLKMLYGN+LH GEKL+TPVHKPPLPSTAF
Sbjct: 61  PAPADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAF 120

Query: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180
           LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV
Sbjct: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180

Query: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240
           GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ
Sbjct: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240

Query: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELER 300
           LPTITTTDSEGFSEKFHVTTLAGKGKRVE TEDKEPDGLSLETVKAAITEKSNLVKELER
Sbjct: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELER 300

Query: 301 SESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHL 360
           SESNREALV AVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNF PQETYLTVSGQLHL
Sbjct: 301 SESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHL 360

Query: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420
           ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW
Sbjct: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420

Query: 421 VLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKF 480
           VLEHCMDDMKFVQKRIDK SIHRLESIISCSFEKVSYT AIEILEKAT+QKS HLKKLKF
Sbjct: 421 VLEHCMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKF 480

Query: 481 GAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540
           GAALT DHLSYLADTIFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG
Sbjct: 481 GAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540

Query: 541 SQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKD 600
           SQKEERLDVLMTRM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLTDIKD
Sbjct: 541 SQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKD 600

Query: 601 VIPFPRSFGKVIN 614
           VIPFPRSFGKV N
Sbjct: 601 VIPFPRSFGKVSN 613

BLAST of MELO3C021113 vs. NCBI nr
Match: KAA0026087.1 (asparagine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 593/617 (96.11%), Postives = 596/617 (96.60%), Query Frame = 0

Query: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAP-- 60
           MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAP  
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  --PAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120
             PAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP
Sbjct: 61  PAPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120

Query: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
           STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180

Query: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
           ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240

Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK 300
           LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK 300

Query: 301 ELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG 360
           ELERSESNREALVAAVQDLKKTN+LALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG
Sbjct: 301 ELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG 360

Query: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420
           QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF
Sbjct: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420

Query: 421 LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLK 480
           LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEK   + S  L 
Sbjct: 421 LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKVKSRLSTLLS 480

Query: 481 KLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540
              F    T +  SYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV
Sbjct: 481 CDSFFLLCTLN--SYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540

Query: 541 VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT 600
           VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT
Sbjct: 541 VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT 600

Query: 601 DIKDVIPFPRSFGKVIN 614
           DIKDVIPFPRSFGKVIN
Sbjct: 601 DIKDVIPFPRSFGKVIN 615

BLAST of MELO3C021113 vs. NCBI nr
Match: XP_038899280.1 (asparagine--tRNA ligase, cytoplasmic 2 [Benincasa hispida])

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 567/619 (91.60%), Postives = 593/619 (95.80%), Query Frame = 0

Query: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60
           MAA+ PTVF+P+TFSKYSNRVLLKTLLDT DGGL+FVGQKLV+GGWVKSSKEVIR+A P+
Sbjct: 1   MAAEHPTVFTPVTFSKYSNRVLLKTLLDTADGGLAFVGQKLVIGGWVKSSKEVIREAAPS 60

Query: 61  PAP------ADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPP 120
           PA       A++LPPK  KDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKL+T V KPP
Sbjct: 61  PADASVRAVANALPPKA-KDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLETAVPKPP 120

Query: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180
           LPSTAFLA+NDGSCVASLQVVVESSLHHPTHL+PTGTCILVEG+LKQVPVPGKHVVRLEV
Sbjct: 121 LPSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKQVPVPGKHVVRLEV 180

Query: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240
           EKILHVGKVEQ+KYPLSQKKLPMDFLRKFSYFRPRTTTVASV RI +ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQQKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQNH 240

Query: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNL 300
           SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGK+VE TEDKE DG+SLETVKAAITEKSNL
Sbjct: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKKVEPTEDKEHDGVSLETVKAAITEKSNL 300

Query: 301 VKELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTV 360
           VKELERSESN+EALVAAVQDLKKTNELALQLEA+EKSK+KSRKDKVIPGNF PQETYLT 
Sbjct: 301 VKELERSESNKEALVAAVQDLKKTNELALQLEAKEKSKTKSRKDKVIPGNFFPQETYLTA 360

Query: 361 SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLV 420
           SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAEL++AMNCADDLV
Sbjct: 361 SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELENAMNCADDLV 420

Query: 421 KFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQH 480
           KFLSKWVL+HC+DDMKFVQKRIDKTSIHRLES+ISCSFEKVSY AAIEILEKATDQKSQH
Sbjct: 421 KFLSKWVLDHCLDDMKFVQKRIDKTSIHRLESVISCSFEKVSYIAAIEILEKATDQKSQH 480

Query: 481 LKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKG 540
           LKKLKFGAALT DHLSYLAD IFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKG
Sbjct: 481 LKKLKFGAALTVDHLSYLADAIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKG 540

Query: 541 GVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTG 600
           GVVISGSQKEER DVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTG
Sbjct: 541 GVVISGSQKEERFDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTG 600

Query: 601 LTDIKDVIPFPRSFGKVIN 614
           LTDIKDVIPFPRSFGKV N
Sbjct: 601 LTDIKDVIPFPRSFGKVSN 618

BLAST of MELO3C021113 vs. ExPASy Swiss-Prot
Match: Q9SW95 (Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 PE=1 SV=2)

HSP 1 Score: 598.6 bits (1542), Expect = 8.0e-170
Identity = 331/626 (52.88%), Postives = 433/626 (69.17%), Query Frame = 0

Query: 11  PITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPAPAPADSLP-P 70
           PIT SKYS RV LKTLLD +D G    G+++V+GGWVKS++ V +++PP P P  + P P
Sbjct: 20  PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79

Query: 71  KLNKD-------VSCIEIIQSRIPIVRSLLKMLYGNN----LHGGEKLDTPVHKPPLPST 130
               D       + C EIIQS++ I +    +L G      +    +L      PP    
Sbjct: 80  SSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKAVPPPEYV 139

Query: 131 AFLAINDGSCVASLQVVVESSLH--HPTHLLPTGTCILVEGVLK-QVPVPGKHVVRLEVE 190
            +  I+DGS ++SLQVVV+S+L     T L+  GTCI+ EGVL+  +    KHV+ LE E
Sbjct: 140 FYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAKHVIELEAE 199

Query: 191 KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNS 250
           K+LHVG V+ EKYPLS+K+LP+  LR FS+FRPRTTTV SVTR+ SALT A+HTF Q + 
Sbjct: 200 KLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQYHG 259

Query: 251 FLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEP-----DGLSLETVKAAITE 310
           F YVQ+P ITTT   GF E F VTTL GK    +  E+K+P     DG S++TVKA I E
Sbjct: 260 FQYVQVPVITTT--TGFGEMFRVTTLLGK---TDDKEEKKPPVQEKDGFSIDTVKAVIKE 319

Query: 311 KSNLVKELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKS--RKDKV-IPGNFLP 370
           K+ L+  L+RS+SNRE +VAAV DLKKTN+LA Q+E ++KSK+ +  + +K+    +F  
Sbjct: 320 KTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDFFG 379

Query: 371 QETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAM 430
           ++TYLT SG+ HLE+YA ALG VY+ GPRF ADKI++A+H AE W VE E+AFAEL DAM
Sbjct: 380 RDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDDAM 439

Query: 431 NCADDLVKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKA 490
           +CAD+  KFL K+VLE+  +DMKF+ KR+DKT   RLE+  S S  + SYT  I +L+KA
Sbjct: 440 DCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQKA 499

Query: 491 TDQKSQHLKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAF 550
           T  K +   K ++G ALT +HLSYL D I+K PVIV+ YPK  K F VRLNDD KTVAAF
Sbjct: 500 TTTKFE--TKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLNDDKKTVAAF 559

Query: 551 DVVLPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDR 610
           D+V+PK GVVI+GSQ EER ++L  R+ E G  RE++EWYL+++RHGTVKHSG SL+ ++
Sbjct: 560 DLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSMEQ 619

Query: 611 FVLFTTGLTDIKDVIPFPRSFGKVIN 614
            +L+ TGL DIKD IPFPRS+GK  N
Sbjct: 620 MLLYATGLPDIKDAIPFPRSWGKANN 638

BLAST of MELO3C021113 vs. ExPASy Swiss-Prot
Match: Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 6.6e-132
Identity = 259/609 (42.53%), Postives = 375/609 (61.58%), Query Frame = 0

Query: 10  SPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPAPAPADSLPP 69
           S +  +++SNRVL++T+LD  DGG    GQ + +GGWVKS ++                 
Sbjct: 22  STVQRAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRD----------------- 81

Query: 70  KLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAFLAINDGSCV 129
                                            G++           + +FLA+NDGSC 
Sbjct: 82  --------------------------------QGKR-----------TFSFLAVNDGSCP 141

Query: 130 ASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGK---HVVRLEVEKILHVGKVEQE 189
           A+LQV+V+ SL+  ++L+ TGTC+ V+GVLK VP  GK     + L V K++ VG V+  
Sbjct: 142 ANLQVMVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDAS 201

Query: 190 KYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITT 249
           KYPL + KL ++ LR   + R RT ++++V RIR+AL FA H+FFQ +SFLY+  P ITT
Sbjct: 202 KYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITT 261

Query: 250 TDSEGFSEKFHVTTLAGKGKRVES--TEDKEPDGLSLETVKAAITEKSNLVKELERSESN 309
           +D EG  E F  TTL    +R+E    ++  P    +E  +  + E+ N+V EL+ ++++
Sbjct: 262 SDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKAS 321

Query: 310 REALVAAVQDLKKTNELALQLEAREKSK-SKSRKDKVI--PGNFLPQETYLTVSGQLHLE 369
           +EA+ AAV +LK   E    ++ R + +    +KD  I    +F  ++ +LTVSGQL +E
Sbjct: 322 KEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVE 381

Query: 370 TYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWV 429
           TYACAL NVY+ GP FRA+   +++H AE WMVE EIAFA+L+D MNCA+  VK++  W+
Sbjct: 382 TYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWL 441

Query: 430 LEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKFG 489
           LE C  DM+ + K  D   I RL+ + S  F +++YT AIE+LE+A  +  +    +++G
Sbjct: 442 LEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGKEFDNNVEWG 501

Query: 490 AALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGS 549
             L ++H  YL + +F+KP+IVYNYPK  K F +RLNDD KTVAA DV++PK G +I GS
Sbjct: 502 IDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGS 561

Query: 550 QKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDV 609
           Q+EER DV+  R++EMGLP E YEWYL+++R+GTVKH GF L F+R +LF TGL +I+DV
Sbjct: 562 QREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRDV 569

Query: 610 IPFPRSFGK 611
           IPFPR  GK
Sbjct: 622 IPFPRYPGK 569

BLAST of MELO3C021113 vs. ExPASy Swiss-Prot
Match: Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 1.6e-122
Identity = 252/611 (41.24%), Postives = 365/611 (59.74%), Query Frame = 0

Query: 10  SPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPAPAPADSLPP 69
           S +  +++S+RVL++++L    GG    GQK+ +GGWVK+ ++  +              
Sbjct: 22  STVQKAQFSDRVLIRSIL---GGGAKLAGQKVRIGGWVKTGRQQGKG------------- 81

Query: 70  KLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAFLAINDGSCV 129
                                                          + AFL +NDGSC 
Sbjct: 82  -----------------------------------------------TFAFLEVNDGSCP 141

Query: 130 ASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHV---VRLEVEKILHVGKVEQE 189
           A+LQV+V+SSL+  + L+ TGTC+ V+GVLK +P  GK +   + L VE ++ VG V+  
Sbjct: 142 ANLQVMVDSSLYDLSRLVATGTCVTVDGVLK-IPPEGKGLKQSIELSVETVIAVGTVDPT 201

Query: 190 KYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITT 249
            YPL + KL  +FLR   + R RT  +++V RIR+AL FA H+FFQ +SFLY+  P ITT
Sbjct: 202 TYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSFLYIHTPIITT 261

Query: 250 TDSEGFSEKFHVTTLAGKGKRVES--TEDKEPDGLSLETVKAAITEKSNLVKELERSESN 309
           +D EG  E F VTTL    +RVE    ++  P    +E  +  + E+   V +L+ ++++
Sbjct: 262 SDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGEAVAQLKVAKAS 321

Query: 310 REALVAAVQDLKKTNELALQLEAREKSKSKSRKD--KVIPGN-FLPQETYLTVSGQLHLE 369
           +E + A+V  L         +E R + K    K+  K+   N F  ++ +LTVSGQL +E
Sbjct: 322 KEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAFLTVSGQLQVE 381

Query: 370 TYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWV 429
           TYACAL +VY+ GP FRA+   +++H AE WMVE EIAFA++ D MNCA+  VK++ KW+
Sbjct: 382 TYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAEAYVKYMCKWL 441

Query: 430 LEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQ-KSQHLKKLKF 489
           ++ C DDM+ + K +D+    RL  +   SF++V+YT AIE LEKA  Q K     K+++
Sbjct: 442 MDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQGKVVFDNKVEW 501

Query: 490 GAALTADHLSYLADTIF-KKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVIS 549
           G  L ++H  YL +  F +KP+IVYNYPK  K F +RLNDD KTVAA DV++PK G +I 
Sbjct: 502 GIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIG 561

Query: 550 GSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIK 609
           GSQ+EER DV+  R++EMGLP E YEWYL+++R+GTVKH GF L F+R + F TG+ +I+
Sbjct: 562 GSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERMIQFATGIDNIR 568

Query: 610 DVIPFPRSFGK 611
           DVIPFPR  GK
Sbjct: 622 DVIPFPRYPGK 568

BLAST of MELO3C021113 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 7.7e-96
Identity = 201/495 (40.61%), Postives = 287/495 (57.98%), Query Frame = 0

Query: 120 FLAINDGSCVASLQVVVESSLHHPTHL---LPTGTCILVEGVLKQVPVPGKHV-VRLEVE 179
           F+ INDGS +++ Q++    +     L   L TG  +   G +  V  PGK   + ++  
Sbjct: 40  FIEINDGSTLSNFQIIATPDIAGYDQLINQLSTGVSVSAIGTI--VESPGKEQNLEMQAT 99

Query: 180 KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNS 239
            I  +GK + E Y L +K+   +FLR  ++ RPRT T+ +VTR+R+AL FA H FFQ   
Sbjct: 100 AITIIGKCDPEVYLLQKKRHTFEFLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQKRG 159

Query: 240 FLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLV 299
           FLY+  P IT +D EG  + F VTTL       ++   + P+G                 
Sbjct: 160 FLYIHTPIITGSDCEGAGKMFQVTTLD------QNNPARTPEG----------------- 219

Query: 300 KELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVS 359
                        V   QD                              F  + TYLTVS
Sbjct: 220 ------------RVDYTQD------------------------------FFGKPTYLTVS 279

Query: 360 GQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVK 419
           GQL+ E YACAL +VY+ GP FRA+   +++H AE WM+E E+AFA+L D M+CA+D +K
Sbjct: 280 GQLNGEIYACALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEMAFADLNDNMDCAEDYLK 339

Query: 420 FLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHL 479
           ++ K+VL++C +DM+F  K +    I RLE +I+ SFE+ SYT A+ ILEKA D+K ++ 
Sbjct: 340 YILKYVLDNCQEDMEFFNKHVATDLISRLEHVINTSFERASYTYAVRILEKA-DKKFEY- 399

Query: 480 KKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGG 539
             +K+G  L ++H  +LA+  F KPVI+ +YPK+ K F +R N+D KTVAA DV++PK G
Sbjct: 400 -PVKWGLDLQSEHERFLAEEFFGKPVILTDYPKDIKAFYMRTNEDNKTVAAMDVLVPKVG 459

Query: 540 VVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGL 599
            +I GSQ+EERL VL +++KE  LP E+Y WYLE+++ G+V HSGF   F+R V FTTG+
Sbjct: 460 EIIGGSQREERLSVLESKLKEFNLPAEEYWWYLELRKFGSVPHSGFGAGFERLVQFTTGM 464

Query: 600 TDIKDVIPFPRSFGK 611
            +I+DVIPFPR  GK
Sbjct: 520 ENIRDVIPFPRHPGK 464

BLAST of MELO3C021113 vs. ExPASy Swiss-Prot
Match: P52276 (Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=asnS PE=3 SV=2)

HSP 1 Score: 341.7 bits (875), Expect = 1.8e-92
Identity = 200/500 (40.00%), Postives = 287/500 (57.40%), Query Frame = 0

Query: 112 KPPLPSTAFLAINDGSCVASLQVVVESSLHHPTHL---LPTGTCILVEGVLKQVPVPGK- 171
           K  L   +F+ +NDGS +A+LQVV++ SL     L   L TG  ++VEG  K  P PGK 
Sbjct: 28  KRTLKDFSFVEVNDGSSLANLQVVLDGSLADYDRLLSQLQTGAALVVEG--KLAPSPGKG 87

Query: 172 HVVRLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFAN 231
             V L+  K+  +G  +   YPL +K+   +FLR   + RPRT T+ +V R+R+A   A 
Sbjct: 88  QRVELKATKLELLGGADPGSYPLQKKRHSFEFLRTIGHLRPRTNTIGAVMRVRNACATAI 147

Query: 232 HTFFQNNSFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAA 291
           H FFQ   FL+V  P IT +D EG  + F VTTL                          
Sbjct: 148 HQFFQERGFLWVHTPIITASDCEGAGDLFKVTTL-------------------------- 207

Query: 292 ITEKSNLVKELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLP 351
                    +L+R   N E +     D  +                          +F  
Sbjct: 208 ---------DLQRVPKNGEGI-----DYSQ--------------------------DFFG 267

Query: 352 QETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAM 411
           ++ YLTVSGQL  E  A A  NVY+ GP FRA+   +++H AE WMVE E+AF +L+   
Sbjct: 268 KQAYLTVSGQLEAEVMALAFQNVYTFGPTFRAENSNTSRHLAEFWMVEPEMAFCDLEGDR 327

Query: 412 NCADDLVKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKA 471
             A++ +K++ K+VLE C +DM+F  +RID T +   ++II+  F  ++Y+ AI++LEKA
Sbjct: 328 QWAEEFLKYIFKFVLEKCPEDMEFFDQRIDNTVLATADNIINNEFAWLTYSEAIKLLEKA 387

Query: 472 TDQKSQHLKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAF 531
            DQK ++   + +G  L ++H  YLA+T+FK+P IV +YPK+ K F +RLNDDGKTVAA 
Sbjct: 388 -DQKFEY--PVAWGVDLQSEHERYLAETVFKRPTIVTDYPKDIKAFYMRLNDDGKTVAAM 447

Query: 532 DVVLPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDR 591
           D++ PK G +I GSQ+EERLD+L  RM+E G+P E   WYL+++R+G+V H+GF L F+R
Sbjct: 448 DILAPKIGEIIGGSQREERLDILTQRMQEQGVPEEDLWWYLDLRRYGSVPHAGFGLGFER 456

Query: 592 FVLFTTGLTDIKDVIPFPRS 608
            V F TG+ +I+DVIPFPR+
Sbjct: 508 IVQFMTGMANIRDVIPFPRT 456

BLAST of MELO3C021113 vs. ExPASy TrEMBL
Match: A0A1S3C6Z5 (Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1)

HSP 1 Score: 1204.5 bits (3115), Expect = 0.0e+00
Identity = 613/613 (100.00%), Postives = 613/613 (100.00%), Query Frame = 0

Query: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60
           MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAF 120
           PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAF
Sbjct: 61  PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAF 120

Query: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180
           LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV
Sbjct: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180

Query: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240
           GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ
Sbjct: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240

Query: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELER 300
           LPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELER
Sbjct: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELER 300

Query: 301 SESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHL 360
           SESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHL
Sbjct: 301 SESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHL 360

Query: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420
           ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW
Sbjct: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420

Query: 421 VLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKF 480
           VLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKF
Sbjct: 421 VLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKF 480

Query: 481 GAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540
           GAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG
Sbjct: 481 GAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540

Query: 541 SQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKD 600
           SQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKD
Sbjct: 541 SQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKD 600

Query: 601 VIPFPRSFGKVIN 614
           VIPFPRSFGKVIN
Sbjct: 601 VIPFPRSFGKVIN 613

BLAST of MELO3C021113 vs. ExPASy TrEMBL
Match: A0A5D3BD07 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1970G00300 PE=3 SV=1)

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 612/617 (99.19%), Postives = 613/617 (99.35%), Query Frame = 0

Query: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAP-- 60
           MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAP  
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  --PAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120
             PAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP
Sbjct: 61  PAPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120

Query: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
           STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180

Query: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
           ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240

Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK 300
           LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK 300

Query: 301 ELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG 360
           ELERSESNREALVAAVQDLKKTN+LALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG
Sbjct: 301 ELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG 360

Query: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420
           QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF
Sbjct: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420

Query: 421 LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLK 480
           LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLK
Sbjct: 421 LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLK 480

Query: 481 KLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540
           KLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV
Sbjct: 481 KLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540

Query: 541 VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT 600
           VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT
Sbjct: 541 VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT 600

Query: 601 DIKDVIPFPRSFGKVIN 614
           DIKDVIPFPRSFGKVIN
Sbjct: 601 DIKDVIPFPRSFGKVIN 617

BLAST of MELO3C021113 vs. ExPASy TrEMBL
Match: A0A0A0K8G1 (Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_7G368100 PE=3 SV=1)

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 595/613 (97.06%), Postives = 601/613 (98.04%), Query Frame = 0

Query: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60
           MAAQQPTVF+PITFSKYSNRVLLKTLLDTTDGGLSFVGQK VVGGWVKSSKEVIRDAPPA
Sbjct: 1   MAAQQPTVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60

Query: 61  PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAF 120
           PAPADSLPPK+NKDVSCIEIIQSRIPIVRSLLKMLYGN+LH GEKL+TPVHKPPLPSTAF
Sbjct: 61  PAPADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAF 120

Query: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180
           LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV
Sbjct: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180

Query: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240
           GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ
Sbjct: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240

Query: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELER 300
           LPTITTTDSEGFSEKFHVTTLAGKGKRVE TEDKEPDGLSLETVKAAITEKSNLVKELER
Sbjct: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELER 300

Query: 301 SESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHL 360
           SESNREALV AVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNF PQETYLTVSGQLHL
Sbjct: 301 SESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHL 360

Query: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420
           ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW
Sbjct: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420

Query: 421 VLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKF 480
           VLEHCMDDMKFVQKRIDK SIHRLESIISCSFEKVSYT AIEILEKAT+QKS HLKKLKF
Sbjct: 421 VLEHCMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKF 480

Query: 481 GAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540
           GAALT DHLSYLADTIFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG
Sbjct: 481 GAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540

Query: 541 SQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKD 600
           SQKEERLDVLMTRM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLTDIKD
Sbjct: 541 SQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKD 600

Query: 601 VIPFPRSFGKVIN 614
           VIPFPRSFGKV N
Sbjct: 601 VIPFPRSFGKVSN 613

BLAST of MELO3C021113 vs. ExPASy TrEMBL
Match: A0A5A7SMX6 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G00190 PE=3 SV=1)

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 593/617 (96.11%), Postives = 596/617 (96.60%), Query Frame = 0

Query: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAP-- 60
           MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAP  
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  --PAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120
             PAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP
Sbjct: 61  PAPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120

Query: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
           STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180

Query: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
           ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240

Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK 300
           LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK 300

Query: 301 ELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG 360
           ELERSESNREALVAAVQDLKKTN+LALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG
Sbjct: 301 ELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG 360

Query: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420
           QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF
Sbjct: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420

Query: 421 LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLK 480
           LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEK   + S  L 
Sbjct: 421 LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKVKSRLSTLLS 480

Query: 481 KLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540
              F    T +  SYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV
Sbjct: 481 CDSFFLLCTLN--SYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540

Query: 541 VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT 600
           VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT
Sbjct: 541 VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT 600

Query: 601 DIKDVIPFPRSFGKVIN 614
           DIKDVIPFPRSFGKVIN
Sbjct: 601 DIKDVIPFPRSFGKVIN 615

BLAST of MELO3C021113 vs. ExPASy TrEMBL
Match: A0A6J1KTV9 (asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497586 PE=4 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 5.4e-294
Identity = 523/620 (84.35%), Postives = 560/620 (90.32%), Query Frame = 0

Query: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAP-P 60
           MAA+ PT F+P+TFSKYS RVLLKT+LD +DGGL FV QKLV+GGWVKSSKEV R+A  P
Sbjct: 1   MAAEHPTAFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  APA---PADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHK--PP 120
           A A    A    P+  KDVSCIEI+QSRIPIVRSLL+MLYGN+LH GEKL+TPVHK  PP
Sbjct: 61  ADASVRTAGDALPRRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETPVHKPPPP 120

Query: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180
           LPST FL +NDGSCVASLQVVVESSLHHP  L+PTGTCILVEG+LK+ P PGKH+VRLEV
Sbjct: 121 LPSTTFLVVNDGSCVASLQVVVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180

Query: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240
           EKILHVGKVEQEKYPL+QKKLPMD LRKFSYFRPRTTTVASV RI +ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQEKYPLTQKKLPMDLLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQNH 240

Query: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKG--KRVESTEDKEPDGLSLETVKAAITEKS 300
            FLYVQLPTITTTDSEGFSEKFHVTT AGK   K++E TEDKE DG+SLETVKAA  EK 
Sbjct: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGKSEDKKIEPTEDKETDGVSLETVKAAAVEKR 300

Query: 301 NLVKELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKV-IPGNFLPQETY 360
           N+VKELERSESNREAL AAVQDLKKTNELALQLEAR K KS+ +KDKV I  NF PQETY
Sbjct: 301 NIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKSQLKKDKVHISENFFPQETY 360

Query: 361 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCAD 420
           LTVSGQLHLETYACALGNVYSCGPRFRAD+I SAKHAAEMWMVEVEIAFAEL+D +NCAD
Sbjct: 361 LTVSGQLHLETYACALGNVYSCGPRFRADRIVSAKHAAEMWMVEVEIAFAELEDVINCAD 420

Query: 421 DLVKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQK 480
           DLVKFLSKWVLEHC+DDMKFVQKRIDKTS+HRLES I+ SFEKVSYTAA+EIL+KATDQK
Sbjct: 421 DLVKFLSKWVLEHCLDDMKFVQKRIDKTSVHRLESTITSSFEKVSYTAAVEILDKATDQK 480

Query: 481 SQHLKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVL 540
           SQ LK+LKFGAALT DHLSYLAD IFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVL
Sbjct: 481 SQRLKRLKFGAALTDDHLSYLADAIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVL 540

Query: 541 PKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF 600
           PKGGVVISGSQKEER DVL++RMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF
Sbjct: 541 PKGGVVISGSQKEERFDVLLSRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLF 600

Query: 601 TTGLTDIKDVIPFPRSFGKV 612
           TTGLTDIKDVIPFPRS GKV
Sbjct: 601 TTGLTDIKDVIPFPRSLGKV 620

BLAST of MELO3C021113 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 598.6 bits (1542), Expect = 5.7e-171
Identity = 331/626 (52.88%), Postives = 433/626 (69.17%), Query Frame = 0

Query: 11  PITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPAPAPADSLP-P 70
           PIT SKYS RV LKTLLD +D G    G+++V+GGWVKS++ V +++PP P P  + P P
Sbjct: 20  PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79

Query: 71  KLNKD-------VSCIEIIQSRIPIVRSLLKMLYGNN----LHGGEKLDTPVHKPPLPST 130
               D       + C EIIQS++ I +    +L G      +    +L      PP    
Sbjct: 80  SSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKAVPPPEYV 139

Query: 131 AFLAINDGSCVASLQVVVESSLH--HPTHLLPTGTCILVEGVLK-QVPVPGKHVVRLEVE 190
            +  I+DGS ++SLQVVV+S+L     T L+  GTCI+ EGVL+  +    KHV+ LE E
Sbjct: 140 FYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAKHVIELEAE 199

Query: 191 KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNS 250
           K+LHVG V+ EKYPLS+K+LP+  LR FS+FRPRTTTV SVTR+ SALT A+HTF Q + 
Sbjct: 200 KLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQYHG 259

Query: 251 FLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEP-----DGLSLETVKAAITE 310
           F YVQ+P ITTT   GF E F VTTL GK    +  E+K+P     DG S++TVKA I E
Sbjct: 260 FQYVQVPVITTT--TGFGEMFRVTTLLGK---TDDKEEKKPPVQEKDGFSIDTVKAVIKE 319

Query: 311 KSNLVKELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKS--RKDKV-IPGNFLP 370
           K+ L+  L+RS+SNRE +VAAV DLKKTN+LA Q+E ++KSK+ +  + +K+    +F  
Sbjct: 320 KTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDFFG 379

Query: 371 QETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAM 430
           ++TYLT SG+ HLE+YA ALG VY+ GPRF ADKI++A+H AE W VE E+AFAEL DAM
Sbjct: 380 RDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDDAM 439

Query: 431 NCADDLVKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKA 490
           +CAD+  KFL K+VLE+  +DMKF+ KR+DKT   RLE+  S S  + SYT  I +L+KA
Sbjct: 440 DCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQKA 499

Query: 491 TDQKSQHLKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAF 550
           T  K +   K ++G ALT +HLSYL D I+K PVIV+ YPK  K F VRLNDD KTVAAF
Sbjct: 500 TTTKFE--TKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLNDDKKTVAAF 559

Query: 551 DVVLPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDR 610
           D+V+PK GVVI+GSQ EER ++L  R+ E G  RE++EWYL+++RHGTVKHSG SL+ ++
Sbjct: 560 DLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSMEQ 619

Query: 611 FVLFTTGLTDIKDVIPFPRSFGKVIN 614
            +L+ TGL DIKD IPFPRS+GK  N
Sbjct: 620 MLLYATGLPDIKDAIPFPRSWGKANN 638

BLAST of MELO3C021113 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 472.6 bits (1215), Expect = 4.7e-133
Identity = 259/609 (42.53%), Postives = 375/609 (61.58%), Query Frame = 0

Query: 10  SPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPAPAPADSLPP 69
           S +  +++SNRVL++T+LD  DGG    GQ + +GGWVKS ++                 
Sbjct: 22  STVQRAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRD----------------- 81

Query: 70  KLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAFLAINDGSCV 129
                                            G++           + +FLA+NDGSC 
Sbjct: 82  --------------------------------QGKR-----------TFSFLAVNDGSCP 141

Query: 130 ASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGK---HVVRLEVEKILHVGKVEQE 189
           A+LQV+V+ SL+  ++L+ TGTC+ V+GVLK VP  GK     + L V K++ VG V+  
Sbjct: 142 ANLQVMVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDAS 201

Query: 190 KYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITT 249
           KYPL + KL ++ LR   + R RT ++++V RIR+AL FA H+FFQ +SFLY+  P ITT
Sbjct: 202 KYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITT 261

Query: 250 TDSEGFSEKFHVTTLAGKGKRVES--TEDKEPDGLSLETVKAAITEKSNLVKELERSESN 309
           +D EG  E F  TTL    +R+E    ++  P    +E  +  + E+ N+V EL+ ++++
Sbjct: 262 SDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKAS 321

Query: 310 REALVAAVQDLKKTNELALQLEAREKSK-SKSRKDKVI--PGNFLPQETYLTVSGQLHLE 369
           +EA+ AAV +LK   E    ++ R + +    +KD  I    +F  ++ +LTVSGQL +E
Sbjct: 322 KEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVE 381

Query: 370 TYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWV 429
           TYACAL NVY+ GP FRA+   +++H AE WMVE EIAFA+L+D MNCA+  VK++  W+
Sbjct: 382 TYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWL 441

Query: 430 LEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKFG 489
           LE C  DM+ + K  D   I RL+ + S  F +++YT AIE+LE+A  +  +    +++G
Sbjct: 442 LEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGKEFDNNVEWG 501

Query: 490 AALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGS 549
             L ++H  YL + +F+KP+IVYNYPK  K F +RLNDD KTVAA DV++PK G +I GS
Sbjct: 502 IDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGS 561

Query: 550 QKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKDV 609
           Q+EER DV+  R++EMGLP E YEWYL+++R+GTVKH GF L F+R +LF TGL +I+DV
Sbjct: 562 QREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRDV 569

Query: 610 IPFPRSFGK 611
           IPFPR  GK
Sbjct: 622 IPFPRYPGK 569

BLAST of MELO3C021113 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 441.4 bits (1134), Expect = 1.2e-123
Identity = 252/611 (41.24%), Postives = 365/611 (59.74%), Query Frame = 0

Query: 10  SPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPAPAPADSLPP 69
           S +  +++S+RVL++++L    GG    GQK+ +GGWVK+ ++  +              
Sbjct: 22  STVQKAQFSDRVLIRSIL---GGGAKLAGQKVRIGGWVKTGRQQGKG------------- 81

Query: 70  KLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAFLAINDGSCV 129
                                                          + AFL +NDGSC 
Sbjct: 82  -----------------------------------------------TFAFLEVNDGSCP 141

Query: 130 ASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHV---VRLEVEKILHVGKVEQE 189
           A+LQV+V+SSL+  + L+ TGTC+ V+GVLK +P  GK +   + L VE ++ VG V+  
Sbjct: 142 ANLQVMVDSSLYDLSRLVATGTCVTVDGVLK-IPPEGKGLKQSIELSVETVIAVGTVDPT 201

Query: 190 KYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITT 249
            YPL + KL  +FLR   + R RT  +++V RIR+AL FA H+FFQ +SFLY+  P ITT
Sbjct: 202 TYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSFLYIHTPIITT 261

Query: 250 TDSEGFSEKFHVTTLAGKGKRVES--TEDKEPDGLSLETVKAAITEKSNLVKELERSESN 309
           +D EG  E F VTTL    +RVE    ++  P    +E  +  + E+   V +L+ ++++
Sbjct: 262 SDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGEAVAQLKVAKAS 321

Query: 310 REALVAAVQDLKKTNELALQLEAREKSKSKSRKD--KVIPGN-FLPQETYLTVSGQLHLE 369
           +E + A+V  L         +E R + K    K+  K+   N F  ++ +LTVSGQL +E
Sbjct: 322 KEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAFLTVSGQLQVE 381

Query: 370 TYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWV 429
           TYACAL +VY+ GP FRA+   +++H AE WMVE EIAFA++ D MNCA+  VK++ KW+
Sbjct: 382 TYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAEAYVKYMCKWL 441

Query: 430 LEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQ-KSQHLKKLKF 489
           ++ C DDM+ + K +D+    RL  +   SF++V+YT AIE LEKA  Q K     K+++
Sbjct: 442 MDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQGKVVFDNKVEW 501

Query: 490 GAALTADHLSYLADTIF-KKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVIS 549
           G  L ++H  YL +  F +KP+IVYNYPK  K F +RLNDD KTVAA DV++PK G +I 
Sbjct: 502 GIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIG 561

Query: 550 GSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIK 609
           GSQ+EER DV+  R++EMGLP E YEWYL+++R+GTVKH GF L F+R + F TG+ +I+
Sbjct: 562 GSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERMIQFATGIDNIR 568

Query: 610 DVIPFPRSFGK 611
           DVIPFPR  GK
Sbjct: 622 DVIPFPRYPGK 568

BLAST of MELO3C021113 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 318.9 bits (816), Expect = 8.7e-87
Identity = 191/497 (38.43%), Postives = 285/497 (57.34%), Query Frame = 0

Query: 117 STAFLAINDGSCVASLQVVVESSLHHPTHL----LPTGTCILVEGVLKQVPVPG-KHVVR 176
           S  F+ INDGSC+++LQ V+ S       +    + TG  + V+G +  V   G K  V 
Sbjct: 126 SVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTI--VASQGTKQKVE 185

Query: 177 LEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFF 236
           L+VEKI+ VG+ +   YP+ +K++  +FLR  ++ RPRT T  +V R+R+ L +A H FF
Sbjct: 186 LKVEKIIVVGECD-SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFF 245

Query: 237 QNNSFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEK 296
           Q + F++V  P IT +D EG  E+F VTTL      + S+ +                  
Sbjct: 246 QESGFVWVASPIITASDCEGAGEQFCVTTL------IPSSHE------------------ 305

Query: 297 SNLVKELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETY 356
            N    ++     +  L+   QD                              F  +  +
Sbjct: 306 -NTDTSIDAIPKTKGGLIDWSQD------------------------------FFGKPAF 365

Query: 357 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCAD 416
           LTVSGQL+ ETYA AL +VY+ GP FRA+   +++H AE WM+E E+AFA+L D M CA 
Sbjct: 366 LTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACAT 425

Query: 417 DLVKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQK 476
             ++++ K+VL++C +DM+F    I+K  I RL  +    F ++ YT AIEIL KA  + 
Sbjct: 426 AYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEILLKANKKF 485

Query: 477 SQHLKKLKFGAALTADHLSYLADTIF-KKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVV 536
                 +K+G  L ++H  Y+ +  F  +PVI+ +YPKE K F +R NDDGKTVAA D++
Sbjct: 486 D---FPVKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDML 545

Query: 537 LPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVL 596
           +P+ G +I GSQ+EERL+VL  R+ E+ L +E Y WYL+++R+G+V H+GF L F+R V 
Sbjct: 546 VPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQ 561

Query: 597 FTTGLTDIKDVIPFPRS 608
           F TG+ +I+DVIPFPR+
Sbjct: 606 FVTGIDNIRDVIPFPRT 561

BLAST of MELO3C021113 vs. TAIR 10
Match: AT1G68420.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 1.4e-15
Identity = 35/77 (45.45%), Postives = 58/77 (75.32%), Query Frame = 0

Query: 367 LGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCM 426
           +G+VY+  P FRA+K  +++H AE WMVEVE+AFA +++AMNC++ +VK +   +LE C 
Sbjct: 1   MGDVYTFAPTFRAEKSHTSRHLAEFWMVEVELAFAGVEEAMNCSEAVVKDMCTTLLEKCR 60

Query: 427 DDMKFVQKRIDKTSIHR 444
           DDM+++ +++D+  I R
Sbjct: 61  DDMEYMVEKVDEFCIDR 77

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008458080.10.0e+00100.00PREDICTED: asparagine--tRNA ligase, cytoplasmic 2 [Cucumis melo][more]
TYJ96325.10.0e+0099.19asparagine--tRNA ligase [Cucumis melo var. makuwa][more]
XP_004149331.10.0e+0097.06asparagine--tRNA ligase, cytoplasmic 2 isoform X1 [Cucumis sativus][more]
KAA0026087.10.0e+0096.11asparagine--tRNA ligase [Cucumis melo var. makuwa][more]
XP_038899280.10.0e+0091.60asparagine--tRNA ligase, cytoplasmic 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9SW958.0e-17052.88Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 ... [more]
Q9SW966.6e-13242.53Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ... [more]
Q9SSK11.6e-12241.24Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ... [more]
Q6MEC97.7e-9640.61Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G... [more]
P522761.8e-9240.00Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117... [more]
Match NameE-valueIdentityDescription
A0A1S3C6Z50.0e+00100.00Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1[more]
A0A5D3BD070.0e+0099.19Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A0A0K8G10.0e+0097.06Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_7G368100 PE=3 SV=1[more]
A0A5A7SMX60.0e+0096.11Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J1KTV95.4e-29484.35asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT3G07420.15.7e-17152.88asparaginyl-tRNA synthetase 2 [more]
AT5G56680.14.7e-13342.53Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT1G70980.11.2e-12341.24Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G17300.18.7e-8738.43Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT1G68420.11.4e-1545.45Class II aaRS and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 281..318
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 189..612
e-value: 4.9E-85
score: 287.8
NoneNo IPR availablePANTHERPTHR22594:SF36ASPARAGINE--TRNA LIGASE, CYTOPLASMIC 2-RELATEDcoord: 16..611
NoneNo IPR availablePANTHERPTHR22594ASPARTYL/LYSYL-TRNA SYNTHETASEcoord: 16..611
NoneNo IPR availableCDDcd04318EcAsnRS_like_Ncoord: 119..182
e-value: 5.68725E-15
score: 68.3609
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 197..607
IPR002312Aspartyl/Asparaginyl-tRNA synthetase, class IIbPRINTSPR01042TRNASYNTHASPcoord: 352..364
score: 41.78
coord: 528..544
score: 27.94
coord: 568..582
score: 33.94
coord: 368..381
score: 32.47
IPR004522Asparagine-tRNA ligaseTIGRFAMTIGR00457TIGR00457coord: 118..609
e-value: 7.2E-126
score: 418.6
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 198..260
e-value: 2.4E-7
score: 30.1
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 342..606
e-value: 7.6E-51
score: 173.1

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C021113.1MELO3C021113.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006421 asparaginyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0004816 asparagine-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0000166 nucleotide binding