MELO3C021069 (gene) Melon (DHL92) v4

Overview
NameMELO3C021069
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionPectinesterase
Locationchr01: 25634983 .. 25641483 (-)
RNA-Seq ExpressionMELO3C021069
SyntenyMELO3C021069
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AACTATGGAAAACACTAAACTTTCATTAGCTCTCTTTGCCACTTTACTCCTCACAAATGCTATTATTGTTAGAGCAAATTCTAATGAAAATGGTGTCATGGATAATATTTCGAGTCTCATAGAAACCTCATGTGCCATTACTCTTTATCCACAACTATGTCACTCAACAATATCCTCCATCATTGGTACATCTAATTTGTTGAGTTTGAAAGATATCGTTCAATTGTCATTGAGTGTGGCTATGGATGCAGCTAAGCTTAACAATGGAAACATAAAAAAACTTACGATGTCCTTTAACAACGTTAGTAAACGAGACAGGATTCCTCTTCATGATTGTGTCAAATCAACCGACAGGACGATTTATGAGCTTGACAAGGCTATAGAGGATTTTCGTGAGTATCCGAACAAGAAATCTCTTACTTCATATGCCGATGACCTCAAAACTTTCCTCAGTTCTGCCATTACTAATCAGGTTTGTAACTGTTTGGATAAATTTTTTGGTTTTCCTTTTTAAAGATGTCTTTGACAGGGATTTTAATGTGTTTAATGGTTTACATATTTTGTTGGGATTCAAGAGAGTGAATTTTTCATATTAAAATTTCTTGTCAAAATCGTTTTGGATCAAGAGTTGAGTAGAAATTTTTGAAAAGTTGAAGAAAAAAGAACAACTTGAAGTACTTTTAGAAGAAATATTTACAAAAGAAAATTCTTATCTTGCATGTTGTGTTTCCAAATTATTTCAGTTAATTCGGTAATTATTTAGTTTTTTTTTAGTTTTAAAAAATGAGGCCTATAAATACTTACATGGCCTCTAAATTGCTTTGTAATTTACTTTTTCCTAAAACCAAATCGGTAAACTAAAACTAGTTTTTGAGAACATGGAATTTTGCTAATATTTTAACTCTTGTACTTGAAAAATATGCATAATTTTTAAAAATCGAAAACCAAAGAGAAATGATTGCTGGACCTTCATTTTTTGTTTTTAGTTTTCGATTTACTAAATTTATTCTCGTATTCTCCTAATTTTCTAATTCAGGGCTTTTACGTATGGTAAGGAAATATCTAAAGTGTTAGTTAAATTGCATGATTATAAACAAAAAGAAGATTGTAAGACTTGACTTGAATTTCACTTGAATTTTGGAAATCTTAATTGAAAATTCATGGAAAACATATAACATACAAATTTAGGGGTGAAAGTACAAGATTAAGTTCAAAAATTAAAAACCAACAATCAAAATCCTTTTTGTTAACCTTTAAATATATTATATTTATTGTTATCTATTTATTAGGTGGTTTCAAAATCATAACCAAAAAATTTGAAACAAAATTATTTATTTTCAAAAACAAAATTGTCTCATAAGTCTAATAACTCTAGACATGAAATATATATGAAAATTACCACGAACAAGAACTTGCATGTAAACAAATGCAATTATTAAAACCAAGTTCAAGTAGATACTCCAACTTCTTCACAAAGGAATATCAAAATACATACTATAAGTGCTTATTTTGATTTGTTTTTTTAGGTATTTTAAGCGCTGAGAGGGTGAAATCAGATATAAAATACATCCAAACAATTCCTAAACGTTTCTAGAGTACTTTAGTATGTTTAAATTTCTGGGGAATTCTTTAAATGAGCTAAAAGAATCCTAAATAAAAACTTGATTATTTTCGATTGCAAATTTATTTACCATCTTGATTATCATGCTTATTTTGAAGTGAACAACATATTGTGTATTTATTTTTGGTTCAGGTGACTTGTTTGGATGGATTCCAAGATGACAAAACAGAGAAAGGGGTTTTGAGAGTTATTGAGAACACTCATATTCATGCGACGAAGTTGTGTAGTAATGCATTGGCTTTGGTTAAAAAATTGACAACAGAAATTGCCTTAATAGATGAGAAAAACTTGGTGGTCCATGACTTCCCTTATAAGATAACTTCGATATCCTCCCAAATAAATGACCTCAAAATTGTCCTATTTTCCAATCAGGTATACTAAAATTTTCAACCTTGTTTTAAACCCTTTTTATTTTTGTTTTATATACATATATCTTCAAATTTTTTACTCATACACGTATGCATCTCAACATTAATATCTTTCAAATTGCATGGATGTTAATAGACACAGATAGATCTATCCACGATAAATGATAGTAGTATTTTATCGGTGACACTTTTTTATATTAGAAAATATTTTGGATCATTTTGTTATATTTGAAAACAACTTTCAATCTTTTTTATATATATATATTTAAAAAATTTACTTTCACTTAAATTCTTAAATTTTCAAAATGTATCTTTTGTCTCCTATTTCTAAGAGCATGTGTGTTCGGTTCGGTTCTCGAGTTTTTAAAATATATCATTTAAGTTCCTATGATTTTAAGAGAATAAGTTTTTAAAAGATCATTAAAGTAGTTTTTATTTTATAAAGTTATTTTTATAAAAGAGCATTGTAAACCTATTCTTGGAAAAATTGTATCTAGGGAAAACTCGGAAAGCAAACAGATATTAAAAAGAATAGAGAACTGTAAAATTAATTTTGATAAAAAATACGATAAAATGCTAAAAATATTTAGAAACTATCGTAAAATTTCATGATAGATATTAATATAATATCATTTTATTGATACAATCTAAAATTTTATTATACATTATAAATATTTTGGTTCATTTGGCTATTTAAAATGTCACAAATAATATTAGTATATAACATTTTCTACAAATATATGGAAGGAGAAATTTTTTTAGTAATAATTAATGTATTTGGATTCCTATATATATATATATATATATCTCGGCTCTACTAGATATAGGGTTTCAGGATCTAATTGAAGACCATTTCTTCGGTGTACATTAGTTTTGATATTGATTGATCTTCATAAGTAAATGAGACATATGATTACATCATAGTTCTATATTTAGCAATTTAGATATAATTAACAGGAGGAAGGTGAGAATGGAAGAAGGGAGGATTTGGAAAATGGAATAAAGTGGCCAGAATGGATGTCAATCCAAGACCAGAAGCTTTTAGAATCATCGTCGTCAGAAGCGGCGGCGGATGTGGTAGTGGCGGTGGATGGAAGCGGAAATTATAAAACGGTGTCGGAAGCAGTGGAGGCGGCGCCGAGCAAGAACAGCAAAAGATATATAATAAAAATAAAGGCTGGAGTTTACAGGGAAAATGTTGACGTACCAAGTAGTAAAAGGAATATTATGTTTTGGGGAGATGGAAGATCCAACACCATTATCACTGCTTATAGAAGTCATGGGAGTGGCTGGTCAACCTTCAATTCCGCCACCGTCGGTAACGTAACTAAACCACCACTCTTTTTCACTGATCCTCATAAATCGGCAATGCAACTAAACTACGTACGTCTGGTGTTATTGAAAGCAAAACTTTGTTAGATTTGGAGTATTTTTAGTTTTTTTTTTCACTAAAGGCTTCCCTTTCTCTTTTATTTTTTATTTTTATTTAAACATCATTTTTAGGAATATTTAAAAACACAAAATAGACCAAAATATTTACTTAAATTTTAAATTTTTATTTATTGTCTATCATTTGCTATATTTTTTTTAAAATATTTTAAATAGTTTTGTTATTTATAATAATTTTTAATTTTTGTAATAATCTTATTTCCTTAAGAAAAGGTAAAATCCAATAATGTCTCAGATATGTTTTTCGTGTTCTACACAAAAATGTTTTATTTTCCTAAGTTTGACTCGACCATTATTCAAAAGGTCAAATAAAGTATTTATATTTGGACATATGGAAGCAATTATCGATCCTAAAGAGACAACTATCTCTTTTTATTTTTCCATTGGTTTTTTTACCCAAATTGCTTACGGAAGAAATGGGTAAACAAAACACTTTAGTTATTTATTTTTTAATTAACCAAGTTTGGATTTTTTTTTTAAATGTTACAAAAAATATTTTTCCAATTATTTAAAATCAATTTTAATTGCTCAATTTTTGGGTTCGTTAATTTCTGTGATAATAATATATACGAATAATAACATTGAGAATCATAAAAATAGTAATTTTGATAAAATATTTACAATATATAGCAAAATTTTAGATTCTATCAATAATAAACATTGTTAGACACTAATAAACTTCTATCAATGATATTGATAGACAATGATAAAAGTTTATCAGTTTTTTTCATTGATAGAATCCAAAATTTTTCTATAGCTTGTAAATATTTTAAATTATTTTGCTATTTTTAAAATTGCCCCTAATAACACTGATTTAGTTATTTCTATACAAAATAGTTTTAAGTAAACAAAGTAACAAAATTTTGAAATTAAATTTTATAACAAGTTCATTAAACGAGTTTTTAAAATAGAAAAAAAGTGACAATTTACCATCTATAAAATAAAACCACACAAAGATAAAATTCATTACACTTGGATTTTTCTACGAAGGATAAATATATCTTGTGAAATGATCTATTTTTTAAGAATTTTCTTTTAAACGTTGTCTATTTTTAAATAAAAAAGTTATCTTTTTGGTGCAATTTCTTTGCTTTTGAGTGTGTAAATGAGTAAAAAGGAGAGAGAGATGAAGAAGAAGAAATGAAAGAGGAGAAACACAAAAACAAAACCTTTTAGTGAACAAACCATGGGTAGTGGGGAATGACGAATATCTGACTTAGCTGTCTGCGAGTCCACAACACAATGCCTTAGCAAGGAAAATCCTAGGCTAAGGGAGATTTTGAAATTTTTTATTTTTCTGAAATTAAAGCAGGAGAAAAGAATGATAAATCCACAGAAAAGAAAAATAAGATAAGAAAGAAAGATGGGGGAAAAAAGTTAACCAAGAGGGTAATGGTCATAAGAGACCCATTTTGCAACAATTGTCAACTATCATCGCATCCATCACAACCTTAAATTAAAAGTGGCATGATAATCGATTGATTACATCGATGACGATCAAGTCGATCGATCGGTCATAATTGGAGTAATGACGTTTAATTGTTAGCAATCAATGGTCAAGCGTCAATGATCAAGACGGTCACTCAACGATGGTAAAGATGGTAAACAGTTGTTAACGACAAAGACGTACGATTATATTAGTAAGTCGTTGACTGTCATGACAATTGGACATTGAGATTCATGGTGTTTAGTTTTTTACCGTCACACGATTTAACAGGCACAACTATTGATCATTAGTAATCAAAACAGTTGGTTGTCAACGATCAAGGTATTCACCAAATGATAGTCACGATTGATGTTATCGGTCACCAACAATCAAGACGATTGATCATCAACAATGACGATCAATGTTATTGGTTACACCAACGGTTAAGAAGATCAATGGTCAATGGTCGATCATGATTGGTCATTGAAGATTCATCACAATAAATCACGATCAATCGTCGACGATCCATCTTGAACGATCACCGACGTTCCATTATGAAGTTGACTAAAATTTGTTGCCAACGGTCGTTGACAACCATCGATAAGAAGTAAAAGTTTTCTAAAACCTATGACATTCAATAAAACCATATTTCGAATTCATTAATTTGGTTTTTTAATTCGAAAAATTTTATTCCAAGTTTCCAAATATGGGCTCAACTTCAAATCAATGCCAAATTTTAAACCCGCGGCCAAATACCCCTAAACATATAATAGTTTCATTAAAAGTACAACAAATTAAAAAGTGATTTTAACCTCTTCGATTATTTATTATTTGTCACAAACCATTTATCATCATTACATTAAAAACACATGCACTTTTTATTAACAAAGTTCTGCAAAAACACTTTAGAATTGAAAAAAAAAAACAATAATAATGCTTGTTTTTTCTTAACAGTTGCTGTGGGGGATGGATTCTTAGCTCGAGACATATCCTTTGAAAACACAGCCGGACCAGCCAACGGCCAAGCTGTAGCCCTCCGTGTTGGCTCAGATCACTCAGCCTTCTACCGCTGTGGCATGCTTGGCTACCAAGACACTCTCTATGTCCACTCGAACCGTCAATTCTTCGTCAACTGCGTTGTAGCAGGAACAGTCGACTTCGTCTTTGGAAACGCAGCAGCTGTAATCCAAAACTCCGACCTCACCCCTCGCAAACCAGGCCCTAACCAAAGGAACATGGTGACGGCACAATCGAGAACTGACTTGAATCAAAACACGGGAATCGTGATCCAAAAGTGTAGGATAAAAGCGACGTCAGACTTGGAGCCAGTGATAAAAGAATTCCCAAGCTTCCTTGGGAGACCATGGGAGGAATATGCTAGGGTTGTGGTAATGCAAACGACAATAACCAACGTGATAGACAAAAAGGGGTGGTCGACATGGAATGGTGACATAAAGAAACCCTACTTTGCAGAATATGACAACAATGGAGCTGGTGCTAATACTTCTGGTAGAGTGCCATGGTCTCTTGTTATTAATGAAGCTGATGCTAAGACCTTCACGGCTGAAAGATTCATTGGCGGTGCTGATTGGCTTCCATCTACTGGCTTCCCTTATCAGCTTGGTCTCTATAACTGA

mRNA sequence

AACTATGGAAAACACTAAACTTTCATTAGCTCTCTTTGCCACTTTACTCCTCACAAATGCTATTATTGTTAGAGCAAATTCTAATGAAAATGGTGTCATGGAAACCTCATGTGCCATTACTCTTTATCCACAACTATGTCACTCAACAATATCCTCCATCATTGGTACATCTAATTTGTTGAGTTTGAAAGATATCGTTCAATTGTCATTGAGTGTGGCTATGGATGCAGCTAAGCTTAACAATGGAAACATAAAAAAACTTACGATGTCCTTTAACAACGTTAGTAAACGAGACAGGATTCCTCTTCATGATTGTGTCAAATCAACCGACAGGACGATTTATGAGCTTGACAAGGCTATAGAGGATTTTCGTGAGTATCCGAACAAGAAATCTCTTACTTCATATGCCGATGACCTCAAAACTTTCCTCAGTTCTGCCATTACTAATCAGGTGACTTGTTTGGATGGATTCCAAGATGACAAAACAGAGAAAGGGGTTTTGAGAGTTATTGAGAACACTCATATTCATGCGACGAAGTTGTGTAGTAATGCATTGGCTTTGGTTAAAAAATTGACAACAGAAATTGCCTTAATAGATGAGAAAAACTTGGTGGTCCATGACTTCCCTTATAAGATAACTTCGATATCCTCCCAAATAAATGACCTCAAAATTGTCCTATTTTCCAATCAGGAGGAAGGTGAGAATGGAAGAAGGGAGGATTTGGAAAATGGAATAAAGTGGCCAGAATGGATGTCAATCCAAGACCAGAAGCTTTTAGAATCATCGTCGTCAGAAGCGGCGGCGGATGTGGTAGTGGCGGTGGATGGAAGCGGAAATTATAAAACGGTGTCGGAAGCAGTGGAGGCGGCGCCGAGCAAGAACAGCAAAAGATATATAATAAAAATAAAGGCTGGAGTTTACAGGGAAAATGTTGACGTACCAAGTAGTAAAAGGAATATTATGTTTTGGGGAGATGGAAGATCCAACACCATTATCACTGCTTATAGAAGTCATGGGAGTGGCTGGTCAACCTTCAATTCCGCCACCGTCGTTGCTGTGGGGGATGGATTCTTAGCTCGAGACATATCCTTTGAAAACACAGCCGGACCAGCCAACGGCCAAGCTGTAGCCCTCCGTGTTGGCTCAGATCACTCAGCCTTCTACCGCTGTGGCATGCTTGGCTACCAAGACACTCTCTATGTCCACTCGAACCGTCAATTCTTCGTCAACTGCGTTGTAGCAGGAACAGTCGACTTCGTCTTTGGAAACGCAGCAGCTGTAATCCAAAACTCCGACCTCACCCCTCGCAAACCAGGCCCTAACCAAAGGAACATGGTGACGGCACAATCGAGAACTGACTTGAATCAAAACACGGGAATCGTGATCCAAAAGTGTAGGATAAAAGCGACGTCAGACTTGGAGCCAGTGATAAAAGAATTCCCAAGCTTCCTTGGGAGACCATGGGAGGAATATGCTAGGGTTGTGGTAATGCAAACGACAATAACCAACGTGATAGACAAAAAGGGGTGGTCGACATGGAATGGTGACATAAAGAAACCCTACTTTGCAGAATATGACAACAATGGAGCTGGTGCTAATACTTCTGGTAGAGTGCCATGGTCTCTTGTTATTAATGAAGCTGATGCTAAGACCTTCACGGCTGAAAGATTCATTGGCGGTGCTGATTGGCTTCCATCTACTGGCTTCCCTTATCAGCTTGGTCTCTATAACTGA

Coding sequence (CDS)

ATGGAAAACACTAAACTTTCATTAGCTCTCTTTGCCACTTTACTCCTCACAAATGCTATTATTGTTAGAGCAAATTCTAATGAAAATGGTGTCATGGAAACCTCATGTGCCATTACTCTTTATCCACAACTATGTCACTCAACAATATCCTCCATCATTGGTACATCTAATTTGTTGAGTTTGAAAGATATCGTTCAATTGTCATTGAGTGTGGCTATGGATGCAGCTAAGCTTAACAATGGAAACATAAAAAAACTTACGATGTCCTTTAACAACGTTAGTAAACGAGACAGGATTCCTCTTCATGATTGTGTCAAATCAACCGACAGGACGATTTATGAGCTTGACAAGGCTATAGAGGATTTTCGTGAGTATCCGAACAAGAAATCTCTTACTTCATATGCCGATGACCTCAAAACTTTCCTCAGTTCTGCCATTACTAATCAGGTGACTTGTTTGGATGGATTCCAAGATGACAAAACAGAGAAAGGGGTTTTGAGAGTTATTGAGAACACTCATATTCATGCGACGAAGTTGTGTAGTAATGCATTGGCTTTGGTTAAAAAATTGACAACAGAAATTGCCTTAATAGATGAGAAAAACTTGGTGGTCCATGACTTCCCTTATAAGATAACTTCGATATCCTCCCAAATAAATGACCTCAAAATTGTCCTATTTTCCAATCAGGAGGAAGGTGAGAATGGAAGAAGGGAGGATTTGGAAAATGGAATAAAGTGGCCAGAATGGATGTCAATCCAAGACCAGAAGCTTTTAGAATCATCGTCGTCAGAAGCGGCGGCGGATGTGGTAGTGGCGGTGGATGGAAGCGGAAATTATAAAACGGTGTCGGAAGCAGTGGAGGCGGCGCCGAGCAAGAACAGCAAAAGATATATAATAAAAATAAAGGCTGGAGTTTACAGGGAAAATGTTGACGTACCAAGTAGTAAAAGGAATATTATGTTTTGGGGAGATGGAAGATCCAACACCATTATCACTGCTTATAGAAGTCATGGGAGTGGCTGGTCAACCTTCAATTCCGCCACCGTCGTTGCTGTGGGGGATGGATTCTTAGCTCGAGACATATCCTTTGAAAACACAGCCGGACCAGCCAACGGCCAAGCTGTAGCCCTCCGTGTTGGCTCAGATCACTCAGCCTTCTACCGCTGTGGCATGCTTGGCTACCAAGACACTCTCTATGTCCACTCGAACCGTCAATTCTTCGTCAACTGCGTTGTAGCAGGAACAGTCGACTTCGTCTTTGGAAACGCAGCAGCTGTAATCCAAAACTCCGACCTCACCCCTCGCAAACCAGGCCCTAACCAAAGGAACATGGTGACGGCACAATCGAGAACTGACTTGAATCAAAACACGGGAATCGTGATCCAAAAGTGTAGGATAAAAGCGACGTCAGACTTGGAGCCAGTGATAAAAGAATTCCCAAGCTTCCTTGGGAGACCATGGGAGGAATATGCTAGGGTTGTGGTAATGCAAACGACAATAACCAACGTGATAGACAAAAAGGGGTGGTCGACATGGAATGGTGACATAAAGAAACCCTACTTTGCAGAATATGACAACAATGGAGCTGGTGCTAATACTTCTGGTAGAGTGCCATGGTCTCTTGTTATTAATGAAGCTGATGCTAAGACCTTCACGGCTGAAAGATTCATTGGCGGTGCTGATTGGCTTCCATCTACTGGCTTCCCTTATCAGCTTGGTCTCTATAACTGA

Protein sequence

MENTKLSLALFATLLLTNAIIVRANSNENGVMETSCAITLYPQLCHSTISSIIGTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTHIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANTSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN
Homology
BLAST of MELO3C021069 vs. NCBI nr
Match: XP_008458040.2 (PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1124.4 bits (2907), Expect = 0.0e+00
Identity = 574/583 (98.46%), Postives = 574/583 (98.46%), Query Frame = 0

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVM-------ETSCAITLYPQLCHSTISSII 60
           MENTKLSLALFATLLLTNAIIVRANSNENGVM       ETSCAITLYPQLCHSTISSII
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDRTIY 120
           GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIK L MSFNNVSKRDRIPLHDCVKSTDRTIY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKNL-MSFNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180
           ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180

Query: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 240
           IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 240

Query: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 300
           NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN
Sbjct: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 300

Query: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360
           SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG
Sbjct: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420
           DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA
Sbjct: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420

Query: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480
           GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540
           PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540

Query: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 577
           SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN
Sbjct: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 582

BLAST of MELO3C021069 vs. NCBI nr
Match: TYK30782.1 (pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.3e-309
Identity = 554/583 (95.03%), Postives = 554/583 (95.03%), Query Frame = 0

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVM-------ETSCAITLYPQLCHSTISSII 60
           MENTKLSLALFATLLLTNAIIVRANSNENGVM       ETSCAITLYPQLCHSTISSII
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDRTIY 120
           GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMS NNVSKRDRIPLHDCVKSTDRTIY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180
           ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEK VLRVIENTH
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTH 180

Query: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 240
           IHATKLCSNALALVKKLTTEIALIDEKNL       K     S       VLFSNQEEGE
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNL------KKSRKARSH------VLFSNQEEGE 240

Query: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 300
           NGRREDLENGIKWPEWMSIQDQKLLESSSSEAA DVVVAVDGSGNYKTVSEAVEAAPSKN
Sbjct: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKN 300

Query: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360
           SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG
Sbjct: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420
           DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA
Sbjct: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420

Query: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480
           GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540
           PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540

Query: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 577
           SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN
Sbjct: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 571

BLAST of MELO3C021069 vs. NCBI nr
Match: KAA0026177.1 (pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1060.4 bits (2741), Expect = 5.3e-306
Identity = 547/583 (93.83%), Postives = 548/583 (94.00%), Query Frame = 0

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVM-------ETSCAITLYPQLCHSTISSII 60
           MENTKLSLALFATLLLTNAIIVRANSNENGVM       ETSCAITLYPQLCHSTISSII
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDRTIY 120
           GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMS NNVSKRDRIPLHDCVKSTDRTIY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180
           ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEK VLRVIENTH
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTH 180

Query: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 240
           IHATKLCSNALALVKKLTTEIALIDEKNL                   K     + EEGE
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNL------------------KKSRKARSHEEGE 240

Query: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 300
           NGRREDLENGIKWPEWMSIQDQKLLESSSSEAA DVVVAVDGSGNYKTVSEAVEAAPSKN
Sbjct: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKN 300

Query: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360
           SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG
Sbjct: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420
           DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA
Sbjct: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420

Query: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480
           GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540
           PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540

Query: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 577
           SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN
Sbjct: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 565

BLAST of MELO3C021069 vs. NCBI nr
Match: XP_011659194.1 (pectinesterase [Cucumis sativus] >KGN44619.1 hypothetical protein Csa_015815 [Cucumis sativus])

HSP 1 Score: 1015.8 bits (2625), Expect = 1.5e-292
Identity = 522/583 (89.54%), Postives = 544/583 (93.31%), Query Frame = 0

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVM-------ETSCAITLYPQLCHSTISSII 60
           MENTKLSLALFATLLLTNAIIVRANSNENGVM       ETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSIL 60

Query: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDRTIY 120
           GTSNLLSLKDIV+LSLSVAMDAAKLNN NIKKL+MS N V KRDRI LHDCVK+TDRTIY
Sbjct: 61  GTSNLLSLKDIVELSLSVAMDAAKLNNENIKKLSMSGNIVGKRDRIALHDCVKTTDRTIY 120

Query: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180
           ELDKAI+DF EYPNKKSL SYADDLKTFLSSAITNQVTCLDG   DKTEK VLR+IENTH
Sbjct: 121 ELDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENTH 180

Query: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 240
              TKLCSNALALVKKLTT++AL DEK+LVVHDFPYKITSISSQI+D KIVLFSNQE+GE
Sbjct: 181 NQVTKLCSNALALVKKLTTDVALTDEKSLVVHDFPYKITSISSQIDDPKIVLFSNQEDGE 240

Query: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 300
           + RREDLENGIKWPEWMSIQDQ LL+SSS E AADVVVA DG+GNYKTVSEAV+AAPSKN
Sbjct: 241 SRRREDLENGIKWPEWMSIQDQMLLDSSSEE-AADVVVAADGTGNYKTVSEAVKAAPSKN 300

Query: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360
           S RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITA RSHGSGWSTFNSATVVAVG
Sbjct: 301 S-RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420
           DGFLARDI+F+NTAG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFV CVVA
Sbjct: 361 DGFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKCVVA 420

Query: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480
           GTVDF+FGNAAAVIQNSDLTPRKPGPNQ NMVTAQSRTDLNQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540
           PVIKEFPSFLGRPWEEYARVVVMQT+ITNVIDK+GWSTWNGDIKKPYFAEYDNNGAGA+T
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGDIKKPYFAEYDNNGAGADT 540

Query: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 577
           SGRV WSLVINEA+AKTFTAE FI GA WLPSTGFPYQLGLYN
Sbjct: 541 SGRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGLYN 581

BLAST of MELO3C021069 vs. NCBI nr
Match: XP_004139620.2 (pectinesterase [Cucumis sativus] >KGN44621.1 hypothetical protein Csa_015743 [Cucumis sativus])

HSP 1 Score: 965.3 bits (2494), Expect = 2.3e-277
Identity = 487/575 (84.70%), Postives = 526/575 (91.48%), Query Frame = 0

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMETSCAITLYPQLCHSTISSIIGTSNLLS 60
           MEN KLSLAL A LLLTNAI+VRANSNEN V+ETSCAITLYPQLCHSTISSI+GTSNLLS
Sbjct: 1   MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS 60

Query: 61  LKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDRTIYELDKAIE 120
           LKDI ++SLSVAMDAAK NN NIKKL +S NNVSKRD+I LHDCV++TDRTIYEL KAIE
Sbjct: 61  LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE 120

Query: 121 DFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTHIHATKLC 180
            FREYPNK+SLT YADDLKTFLSSAITNQVTCLDG   DKTEK VLR+IEN HIH TKLC
Sbjct: 121 VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLC 180

Query: 181 SNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGENGRREDL 240
           SNALALV+KLTT+IA+ DEK+LVVHDFPYKITSI SQ++D KIVLFSNQEE EN RRE+L
Sbjct: 181 SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL 240

Query: 241 ENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKNSKRYIIK 300
           E+GIKWP+WMSI+DQKLLE SSSEAAA+ VVA DGSGNYKTV+EAV AAPSKNSKRYIIK
Sbjct: 241 EDGIKWPKWMSIEDQKLLE-SSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIK 300

Query: 301 IKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDGFLARD 360
           IKAG Y ENVDVPSSKRNIMFWGDGRSNT I + RSHG+GWSTF SAT+ AVGDGFLARD
Sbjct: 301 IKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARD 360

Query: 361 ISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGTVDFVF 420
           I+F+N AG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFVNC+VAGTVDF+F
Sbjct: 361 ITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIF 420

Query: 421 GNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPVIKEFP 480
           GNAAAV QNSD+TPRKPGP+QRNMVTAQSRTD+NQNTGIVIQKCRIKATSDLEPVI+EFP
Sbjct: 421 GNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFP 480

Query: 481 SFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANTSGRVPWS 540
           SFLGRPWEEYARVVVMQTTI+NVIDK+GWSTWNG  K PY+AEYDNNGAGA+ SGRVPWS
Sbjct: 481 SFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWS 540

Query: 541 LVINEADAKTFTAERFIGGADWLPSTGFPYQLGLY 576
           LVI+EA AKTFTA  FIGGADWL STGFPYQL LY
Sbjct: 541 LVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLY 574

BLAST of MELO3C021069 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 1.1e-157
Identity = 301/596 (50.50%), Postives = 403/596 (67.62%), Query Frame = 0

Query: 5   KLSLALFATLLLTNAI-------------------IVRANSNENGVMETSCAITLYPQLC 64
           K+ LA+ A++LL  A+                   I+  +S+ + +++++C+ TL+P+LC
Sbjct: 20  KIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLHPELC 79

Query: 65  HSTISSIIGTS-NLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDC 124
           +S I ++   S  + S KD+++LSL++ + A + N   +K+L  +   ++ R+++ LHDC
Sbjct: 80  YSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVALHDC 139

Query: 125 VKSTDRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKG 184
           +++ D T+ EL  A+ED   YPNKKSL  + +DLKT +SSAITNQ TCLDGF  D+ +K 
Sbjct: 140 LETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDEADKK 199

Query: 185 VLRVIENTHIHATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKI 244
           V +V+     H  K+CSNALA++  +T T+IA                       N++K+
Sbjct: 200 VRKVLLKGQKHVEKMCSNALAMICNMTDTDIA-----------------------NEMKL 259

Query: 245 VLFSNQEEGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVS 304
              +N        R+ +E+  +WPEW+S  D++LL+SS+     DVVVA DGSG+YKTVS
Sbjct: 260 SAPAN-------NRKLVEDNGEWPEWLSAGDRRLLQSST--VTPDVVVAADGSGDYKTVS 319

Query: 305 EAVEAAPSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWST 364
           EAV  AP K+SKRY+I+IKAGVYRENVDVP  K NIMF GDG+SNTIITA R+   G +T
Sbjct: 320 EAVRKAPEKSSKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTT 379

Query: 365 FNSATVVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSN 424
           F+SATVV V    LARDI+F+NTAG +  QAVAL VGSD SAFYRC ML YQDTLYVHSN
Sbjct: 380 FHSATVVRVAGKVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSN 439

Query: 425 RQFFVNCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQK 484
           RQFFV C+VAGTVDF+FGN AAV Q+ D+  R+PG  Q+NMVTAQ RTD NQNTGIVIQK
Sbjct: 440 RQFFVQCLVAGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQK 499

Query: 485 CRIKATSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYF 544
           CRI ATSDL PV K FP++LGRPW+EY+R V+MQ++IT+VI   GW  WNG+  +   ++
Sbjct: 500 CRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFY 559

Query: 545 AEYDNNGAGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 575
            EY N GAGA TSGRV W    ++ +  +A+ +T  RFI G  WL STGFP+ LGL
Sbjct: 560 GEYANTGAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of MELO3C021069 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 1.9e-157
Identity = 300/589 (50.93%), Postives = 399/589 (67.74%), Query Frame = 0

Query: 5   KLSLALFATLLLTNAII-------VRANSNENG------VMETSCAITLYPQLCHSTISS 64
           KL LALFATLL+  A+I        R NS +NG      ++++SC+ T YP LC S I++
Sbjct: 29  KLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAA 88

Query: 65  IIGTS-NLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDR 124
           +   S  + S KD++++SL++   A + N   I+KL +   N++KR+++ LHDC+++ D 
Sbjct: 89  VPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-LKRTNLTKREKVALHDCLETIDE 148

Query: 125 TIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIE 184
           T+ EL KA+ED  EYPNKKSL+ +ADDLKT +S+A+TNQ TCLDGF  D   K V   + 
Sbjct: 149 TLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALS 208

Query: 185 NTHIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQE 244
           +  +H  K+CSNALA++K +T    +I   +                 N+ K++  ++  
Sbjct: 209 DGQVHVEKMCSNALAMIKNMTDTDMMIMRTS-----------------NNRKLIEETSTV 268

Query: 245 EGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAP 304
           +G             WP W+S  D++LL+SSS     +VVVA DGSGN+KTV+ +V AAP
Sbjct: 269 DG-------------WPAWLSTGDRRLLQSSS--VTPNVVVAADGSGNFKTVAASVAAAP 328

Query: 305 SKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVV 364
              +KRYII+IKAGVYRENV+V    +NIMF GDGR+ TIIT  R+   G +TF SATV 
Sbjct: 329 QGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVA 388

Query: 365 AVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNC 424
            VG+GFLARDI+F+NTAGP+  QAVALRVG+D SAFY C ML YQDTLYVHSNRQFFVNC
Sbjct: 389 VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 448

Query: 425 VVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATS 484
           ++AGTVDF+FGNAAAV+QN D+  RKP   Q+NMVTAQ R D NQNTGIVIQK RI ATS
Sbjct: 449 LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATS 508

Query: 485 DLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNG 544
           DL+PV   FP++LGRPW+EY+R V+MQ++IT+VI   GW  W+G+  +   ++ E+ N G
Sbjct: 509 DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAG 568

Query: 545 AGANTSGRVPWS---LVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 575
           AGA TSGRV W    ++ +  +A+ FT   FI G+ WL STGFP+ LGL
Sbjct: 569 AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of MELO3C021069 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 4.3e-157
Identity = 300/589 (50.93%), Postives = 396/589 (67.23%), Query Frame = 0

Query: 5   KLSLALFATLLLTNAII-------VRANSNENG------VMETSCAITLYPQLCHSTISS 64
           KL LALFATLL+  A+I        R NS +NG      ++++SC+ T YP LC S I++
Sbjct: 29  KLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAA 88

Query: 65  IIGTS-NLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDR 124
           +   S  + S KD++++SL++   A + N   I+KL +   N++KR+++ LHDC+++ D 
Sbjct: 89  VPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-LKRTNLTKREKVALHDCLETIDE 148

Query: 125 TIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIE 184
           T+ EL KA+ED  EYPNKKSL+ +ADDLKT +S+A+TNQ TCLDGF  D   K V   + 
Sbjct: 149 TLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALS 208

Query: 185 NTHIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQE 244
           +  +H  K+CSNALA++K +T                            D+ I+  SN  
Sbjct: 209 DGQVHVEKMCSNALAMIKNMTD--------------------------TDMMIMRTSNNR 268

Query: 245 EGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAP 304
           +      E+      WP W+S  D++LL+SSS     + VVA DGSGN+KTV+ AV AAP
Sbjct: 269 K----LTEETSTVDGWPAWLSPGDRRLLQSSS--VTPNAVVAADGSGNFKTVAAAVAAAP 328

Query: 305 SKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVV 364
              +KRYII+IKAGVYRENV+V    +NIMF GDGR+ TIIT  R+   G +TF SAT  
Sbjct: 329 QGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAA 388

Query: 365 AVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNC 424
            VG+GFLARDI+F+NTAGP+  QAVALRVG+D SAFY C ML YQDTLYVHSNRQFFVNC
Sbjct: 389 VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 448

Query: 425 VVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATS 484
           ++AGTVDF+FGNAAAV+QN D+  RKP   Q+NMVTAQ RTD NQNTGIVIQK RI ATS
Sbjct: 449 LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATS 508

Query: 485 DLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNG 544
           DL+PV   FP++LGRPW+EY+R V+MQ++IT++I   GW  W+G+  +   ++ E+ N+G
Sbjct: 509 DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSG 568

Query: 545 AGANTSGRVPWS---LVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 575
           AGA TSGRV W    ++ +  +A+ FT   FI G+ WL STGFP+ LGL
Sbjct: 569 AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of MELO3C021069 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 525.0 bits (1351), Expect = 1.1e-147
Identity = 293/596 (49.16%), Postives = 388/596 (65.10%), Query Frame = 0

Query: 2   ENTKLSLALFATLLLTNAIIV-------RANS------NENGVMETSCAITLYPQLCHST 61
           +N KL L   A  LL  A +        +AN       + + V+ +SC+ T YP+LC S 
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 62  ISSIIGTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKST 121
           + +  G   L S KD+++ S+++ + A + N   +KKL      ++ R++  LHDC+++ 
Sbjct: 76  VVT-AGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETI 135

Query: 122 DRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRV 181
           D T+ EL + +ED   YP KK+L  +A DLKT +SSAITNQ TCLDGF  D  +K V + 
Sbjct: 136 DETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA 195

Query: 182 IENTHIHATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFS 241
           +    IH   +CSNALA++K +T T+IA  ++K         KITS + ++ +       
Sbjct: 196 LLKGQIHVEHMCSNALAMIKNMTDTDIANFEQK--------AKITSNNRKLKE------E 255

Query: 242 NQEE----GENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVS 301
           NQE        G  E    G  WP W+S  D++LL+ S     AD  VA DGSG +KTV+
Sbjct: 256 NQETTVAVDIAGAGELDSEG--WPTWLSAGDRRLLQGSG--VKADATVAADGSGTFKTVA 315

Query: 302 EAVEAAPSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWST 361
            AV AAP  ++KRY+I IKAGVYRENV+V   K+NIMF GDGR+ TIIT  R+   G +T
Sbjct: 316 AAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTT 375

Query: 362 FNSATVVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSN 421
           F+SATV AVG+ FLARDI+F+NTAGP+  QAVALRVGSD SAFY C ML YQDTLYVHSN
Sbjct: 376 FHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSN 435

Query: 422 RQFFVNCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQK 481
           RQFFV C++AGTVDF+FGNAA V+Q+ D+  R+P   Q+NMVTAQ RTD NQNTGIVIQK
Sbjct: 436 RQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQK 495

Query: 482 CRIKATSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYF 541
           CRI ATSDL+ V   FP++LGRPW+EY++ V+MQ+ I++VI  +GWS W G   +    +
Sbjct: 496 CRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTY 555

Query: 542 AEYDNNGAGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 575
            EY N GAGA T+ RV W    ++   A+A+ +TA +FIGG  WL STGFP+ LGL
Sbjct: 556 REYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of MELO3C021069 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 513.8 bits (1322), Expect = 2.4e-144
Identity = 283/589 (48.05%), Postives = 384/589 (65.20%), Query Frame = 0

Query: 6   LSLALFATLLLTNAIIVRA---NSNEN-----------GVMETSCAITLYPQLCHSTISS 65
           LS A  A LLL + + + A   N N+N            ++++ C+ TLYP+LC S +++
Sbjct: 22  LSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCFSAVAA 81

Query: 66  IIGTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDRT 125
             G   L S K++++ SL++   A K N   +KKL      ++ R+   LHDC+++ D T
Sbjct: 82  -TGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLETIDET 141

Query: 126 IYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIEN 185
           + EL  A+ED  +YP +KSL  +ADDLKT +SSAITNQ TCLDGF  D  ++ V + +  
Sbjct: 142 LDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVRKALLK 201

Query: 186 THIHATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQE 245
             +H   +CSNALA++K +T T+IA     N  + D     T+ +++   LK V      
Sbjct: 202 GQVHVEHMCSNALAMIKNMTETDIA-----NFELRDKSSTFTNNNNR--KLKEVTGDLDS 261

Query: 246 EGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAP 305
           +G             WP+W+S+ D++LL+ S+ +  AD  VA DGSG++ TV+ AV AAP
Sbjct: 262 DG-------------WPKWLSVGDRRLLQGSTIK--ADATVADDGSGDFTTVAAAVAAAP 321

Query: 306 SKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVV 365
            K++KR++I IKAGVYRENV+V   K NIMF GDGR  TIIT  R+   G +TF+SATV 
Sbjct: 322 EKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVA 381

Query: 366 AVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNC 425
           AVG+ FLARDI+F+NTAGP+  QAVALRVGSD SAFY+C M  YQDTLYVHSNRQFFV C
Sbjct: 382 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKC 441

Query: 426 VVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATS 485
            + GTVDF+FGNAAAV+Q+ D+  R+P   Q+NMVTAQ R+D NQNTGIVIQ CRI  TS
Sbjct: 442 HITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTS 501

Query: 486 DLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNG 545
           DL  V   FP++LGRPW+EY+R V+MQ+ I++VI  +GW  W+G   +    + EY N G
Sbjct: 502 DLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRG 561

Query: 546 AGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 575
            GA T+ RV W    ++ ++ +A+ FTA +FIGG  WL STGFP+ L L
Sbjct: 562 GGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of MELO3C021069 vs. ExPASy TrEMBL
Match: A0A1S3C861 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497582 PE=3 SV=1)

HSP 1 Score: 1124.4 bits (2907), Expect = 0.0e+00
Identity = 574/583 (98.46%), Postives = 574/583 (98.46%), Query Frame = 0

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVM-------ETSCAITLYPQLCHSTISSII 60
           MENTKLSLALFATLLLTNAIIVRANSNENGVM       ETSCAITLYPQLCHSTISSII
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDRTIY 120
           GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIK L MSFNNVSKRDRIPLHDCVKSTDRTIY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKNL-MSFNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180
           ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180

Query: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 240
           IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 240

Query: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 300
           NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN
Sbjct: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 300

Query: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360
           SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG
Sbjct: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420
           DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA
Sbjct: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420

Query: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480
           GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540
           PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540

Query: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 577
           SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN
Sbjct: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 582

BLAST of MELO3C021069 vs. ExPASy TrEMBL
Match: A0A5D3E4E8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold343G001330 PE=3 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 6.4e-310
Identity = 554/583 (95.03%), Postives = 554/583 (95.03%), Query Frame = 0

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVM-------ETSCAITLYPQLCHSTISSII 60
           MENTKLSLALFATLLLTNAIIVRANSNENGVM       ETSCAITLYPQLCHSTISSII
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDRTIY 120
           GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMS NNVSKRDRIPLHDCVKSTDRTIY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180
           ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEK VLRVIENTH
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTH 180

Query: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 240
           IHATKLCSNALALVKKLTTEIALIDEKNL       K     S       VLFSNQEEGE
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNL------KKSRKARSH------VLFSNQEEGE 240

Query: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 300
           NGRREDLENGIKWPEWMSIQDQKLLESSSSEAA DVVVAVDGSGNYKTVSEAVEAAPSKN
Sbjct: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKN 300

Query: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360
           SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG
Sbjct: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420
           DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA
Sbjct: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420

Query: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480
           GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540
           PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540

Query: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 577
           SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN
Sbjct: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 571

BLAST of MELO3C021069 vs. ExPASy TrEMBL
Match: A0A5A7SNA2 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G001370 PE=3 SV=1)

HSP 1 Score: 1060.4 bits (2741), Expect = 2.6e-306
Identity = 547/583 (93.83%), Postives = 548/583 (94.00%), Query Frame = 0

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVM-------ETSCAITLYPQLCHSTISSII 60
           MENTKLSLALFATLLLTNAIIVRANSNENGVM       ETSCAITLYPQLCHSTISSII
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 60

Query: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDRTIY 120
           GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMS NNVSKRDRIPLHDCVKSTDRTIY
Sbjct: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIY 120

Query: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180
           ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEK VLRVIENTH
Sbjct: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTH 180

Query: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 240
           IHATKLCSNALALVKKLTTEIALIDEKNL                   K     + EEGE
Sbjct: 181 IHATKLCSNALALVKKLTTEIALIDEKNL------------------KKSRKARSHEEGE 240

Query: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 300
           NGRREDLENGIKWPEWMSIQDQKLLESSSSEAA DVVVAVDGSGNYKTVSEAVEAAPSKN
Sbjct: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKN 300

Query: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360
           SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG
Sbjct: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420
           DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA
Sbjct: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420

Query: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480
           GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540
           PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540

Query: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 577
           SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN
Sbjct: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 565

BLAST of MELO3C021069 vs. ExPASy TrEMBL
Match: A0A0A0K6S6 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343850 PE=3 SV=1)

HSP 1 Score: 1015.8 bits (2625), Expect = 7.3e-293
Identity = 522/583 (89.54%), Postives = 544/583 (93.31%), Query Frame = 0

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVM-------ETSCAITLYPQLCHSTISSII 60
           MENTKLSLALFATLLLTNAIIVRANSNENGVM       ETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSIL 60

Query: 61  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDRTIY 120
           GTSNLLSLKDIV+LSLSVAMDAAKLNN NIKKL+MS N V KRDRI LHDCVK+TDRTIY
Sbjct: 61  GTSNLLSLKDIVELSLSVAMDAAKLNNENIKKLSMSGNIVGKRDRIALHDCVKTTDRTIY 120

Query: 121 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 180
           ELDKAI+DF EYPNKKSL SYADDLKTFLSSAITNQVTCLDG   DKTEK VLR+IENTH
Sbjct: 121 ELDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENTH 180

Query: 181 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 240
              TKLCSNALALVKKLTT++AL DEK+LVVHDFPYKITSISSQI+D KIVLFSNQE+GE
Sbjct: 181 NQVTKLCSNALALVKKLTTDVALTDEKSLVVHDFPYKITSISSQIDDPKIVLFSNQEDGE 240

Query: 241 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 300
           + RREDLENGIKWPEWMSIQDQ LL+SSS E AADVVVA DG+GNYKTVSEAV+AAPSKN
Sbjct: 241 SRRREDLENGIKWPEWMSIQDQMLLDSSSEE-AADVVVAADGTGNYKTVSEAVKAAPSKN 300

Query: 301 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 360
           S RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITA RSHGSGWSTFNSATVVAVG
Sbjct: 301 S-RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVVAVG 360

Query: 361 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 420
           DGFLARDI+F+NTAG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFV CVVA
Sbjct: 361 DGFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKCVVA 420

Query: 421 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480
           GTVDF+FGNAAAVIQNSDLTPRKPGPNQ NMVTAQSRTDLNQNTGIVIQKCRIKATSDLE
Sbjct: 421 GTVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 480

Query: 481 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 540
           PVIKEFPSFLGRPWEEYARVVVMQT+ITNVIDK+GWSTWNGDIKKPYFAEYDNNGAGA+T
Sbjct: 481 PVIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGDIKKPYFAEYDNNGAGADT 540

Query: 541 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLYN 577
           SGRV WSLVINEA+AKTFTAE FI GA WLPSTGFPYQLGLYN
Sbjct: 541 SGRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGLYN 581

BLAST of MELO3C021069 vs. ExPASy TrEMBL
Match: A0A0A0K9Y3 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343870 PE=3 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.1e-277
Identity = 487/575 (84.70%), Postives = 526/575 (91.48%), Query Frame = 0

Query: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMETSCAITLYPQLCHSTISSIIGTSNLLS 60
           MEN KLSLAL A LLLTNAI+VRANSNEN V+ETSCAITLYPQLCHSTISSI+GTSNLLS
Sbjct: 1   MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS 60

Query: 61  LKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDRTIYELDKAIE 120
           LKDI ++SLSVAMDAAK NN NIKKL +S NNVSKRD+I LHDCV++TDRTIYEL KAIE
Sbjct: 61  LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE 120

Query: 121 DFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTHIHATKLC 180
            FREYPNK+SLT YADDLKTFLSSAITNQVTCLDG   DKTEK VLR+IEN HIH TKLC
Sbjct: 121 VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLC 180

Query: 181 SNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGENGRREDL 240
           SNALALV+KLTT+IA+ DEK+LVVHDFPYKITSI SQ++D KIVLFSNQEE EN RRE+L
Sbjct: 181 SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL 240

Query: 241 ENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKNSKRYIIK 300
           E+GIKWP+WMSI+DQKLLE SSSEAAA+ VVA DGSGNYKTV+EAV AAPSKNSKRYIIK
Sbjct: 241 EDGIKWPKWMSIEDQKLLE-SSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIK 300

Query: 301 IKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDGFLARD 360
           IKAG Y ENVDVPSSKRNIMFWGDGRSNT I + RSHG+GWSTF SAT+ AVGDGFLARD
Sbjct: 301 IKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARD 360

Query: 361 ISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGTVDFVF 420
           I+F+N AG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFVNC+VAGTVDF+F
Sbjct: 361 ITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIF 420

Query: 421 GNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPVIKEFP 480
           GNAAAV QNSD+TPRKPGP+QRNMVTAQSRTD+NQNTGIVIQKCRIKATSDLEPVI+EFP
Sbjct: 421 GNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFP 480

Query: 481 SFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANTSGRVPWS 540
           SFLGRPWEEYARVVVMQTTI+NVIDK+GWSTWNG  K PY+AEYDNNGAGA+ SGRVPWS
Sbjct: 481 SFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWS 540

Query: 541 LVINEADAKTFTAERFIGGADWLPSTGFPYQLGLY 576
           LVI+EA AKTFTA  FIGGADWL STGFPYQL LY
Sbjct: 541 LVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLY 574

BLAST of MELO3C021069 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 525.0 bits (1351), Expect = 7.5e-149
Identity = 293/596 (49.16%), Postives = 388/596 (65.10%), Query Frame = 0

Query: 2   ENTKLSLALFATLLLTNAIIV-------RANS------NENGVMETSCAITLYPQLCHST 61
           +N KL L   A  LL  A +        +AN       + + V+ +SC+ T YP+LC S 
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 62  ISSIIGTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKST 121
           + +  G   L S KD+++ S+++ + A + N   +KKL      ++ R++  LHDC+++ 
Sbjct: 76  VVT-AGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETI 135

Query: 122 DRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRV 181
           D T+ EL + +ED   YP KK+L  +A DLKT +SSAITNQ TCLDGF  D  +K V + 
Sbjct: 136 DETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA 195

Query: 182 IENTHIHATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFS 241
           +    IH   +CSNALA++K +T T+IA  ++K         KITS + ++ +       
Sbjct: 196 LLKGQIHVEHMCSNALAMIKNMTDTDIANFEQK--------AKITSNNRKLKE------E 255

Query: 242 NQEE----GENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVS 301
           NQE        G  E    G  WP W+S  D++LL+ S     AD  VA DGSG +KTV+
Sbjct: 256 NQETTVAVDIAGAGELDSEG--WPTWLSAGDRRLLQGSG--VKADATVAADGSGTFKTVA 315

Query: 302 EAVEAAPSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWST 361
            AV AAP  ++KRY+I IKAGVYRENV+V   K+NIMF GDGR+ TIIT  R+   G +T
Sbjct: 316 AAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTT 375

Query: 362 FNSATVVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSN 421
           F+SATV AVG+ FLARDI+F+NTAGP+  QAVALRVGSD SAFY C ML YQDTLYVHSN
Sbjct: 376 FHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSN 435

Query: 422 RQFFVNCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQK 481
           RQFFV C++AGTVDF+FGNAA V+Q+ D+  R+P   Q+NMVTAQ RTD NQNTGIVIQK
Sbjct: 436 RQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQK 495

Query: 482 CRIKATSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYF 541
           CRI ATSDL+ V   FP++LGRPW+EY++ V+MQ+ I++VI  +GWS W G   +    +
Sbjct: 496 CRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTY 555

Query: 542 AEYDNNGAGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 575
            EY N GAGA T+ RV W    ++   A+A+ +TA +FIGG  WL STGFP+ LGL
Sbjct: 556 REYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of MELO3C021069 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 513.8 bits (1322), Expect = 1.7e-145
Identity = 283/589 (48.05%), Postives = 384/589 (65.20%), Query Frame = 0

Query: 6   LSLALFATLLLTNAIIVRA---NSNEN-----------GVMETSCAITLYPQLCHSTISS 65
           LS A  A LLL + + + A   N N+N            ++++ C+ TLYP+LC S +++
Sbjct: 22  LSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCFSAVAA 81

Query: 66  IIGTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSKRDRIPLHDCVKSTDRT 125
             G   L S K++++ SL++   A K N   +KKL      ++ R+   LHDC+++ D T
Sbjct: 82  -TGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLETIDET 141

Query: 126 IYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIEN 185
           + EL  A+ED  +YP +KSL  +ADDLKT +SSAITNQ TCLDGF  D  ++ V + +  
Sbjct: 142 LDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVRKALLK 201

Query: 186 THIHATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQE 245
             +H   +CSNALA++K +T T+IA     N  + D     T+ +++   LK V      
Sbjct: 202 GQVHVEHMCSNALAMIKNMTETDIA-----NFELRDKSSTFTNNNNR--KLKEVTGDLDS 261

Query: 246 EGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAP 305
           +G             WP+W+S+ D++LL+ S+ +  AD  VA DGSG++ TV+ AV AAP
Sbjct: 262 DG-------------WPKWLSVGDRRLLQGSTIK--ADATVADDGSGDFTTVAAAVAAAP 321

Query: 306 SKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVV 365
            K++KR++I IKAGVYRENV+V   K NIMF GDGR  TIIT  R+   G +TF+SATV 
Sbjct: 322 EKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVA 381

Query: 366 AVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNC 425
           AVG+ FLARDI+F+NTAGP+  QAVALRVGSD SAFY+C M  YQDTLYVHSNRQFFV C
Sbjct: 382 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKC 441

Query: 426 VVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATS 485
            + GTVDF+FGNAAAV+Q+ D+  R+P   Q+NMVTAQ R+D NQNTGIVIQ CRI  TS
Sbjct: 442 HITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTS 501

Query: 486 DLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNG 545
           DL  V   FP++LGRPW+EY+R V+MQ+ I++VI  +GW  W+G   +    + EY N G
Sbjct: 502 DLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRG 561

Query: 546 AGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 575
            GA T+ RV W    ++ ++ +A+ FTA +FIGG  WL STGFP+ L L
Sbjct: 562 GGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of MELO3C021069 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 351.7 bits (901), Expect = 1.1e-96
Identity = 206/520 (39.62%), Postives = 296/520 (56.92%), Query Frame = 0

Query: 60  SLKDIVQLSLSVAMDAAKLNNGNIKKL-TMSFNNVSKRDRIPLHDCVKSTDRTIYELDKA 119
           ++ +++   L++ +    L + N   L T  F N++  +R    DC+   D TI +L+ A
Sbjct: 52  TIPELIIADLNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETA 111

Query: 120 IEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTHIHATK 179
           + D R      S +   +D+   L++ +T Q TCLDGF             EN +    +
Sbjct: 112 VSDLR------SSSLEFNDISMLLTNVMTYQDTCLDGFSTSDN--------ENNNDMTYE 171

Query: 180 LCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGENGRRE 239
           L  N    +K++  +I+                 ++S+ ++ L+++  S ++        
Sbjct: 172 LPEN----LKEIILDIS----------------NNLSNSLHMLQVI--SRKKPSPKSSEV 231

Query: 240 DLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKNSKRYI 299
           D+E    +P W+S  DQ+LLE+   E   ++ VA+DG+GN+ T+++AV AAP+ +  R+I
Sbjct: 232 DVE----YPSWLSENDQRLLEAPVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFI 291

Query: 300 IKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDGFLA 359
           I IK G Y ENV++P  K  IMF GDG   T+I A RS   GWSTF + TV   G G++A
Sbjct: 292 IYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIA 351

Query: 360 RDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGTVDF 419
           +DISF N+AGPA  QAVA R GSDHSAFYRC   GYQDTLYVHS +QF+  C + GT+DF
Sbjct: 352 KDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDF 411

Query: 420 VFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPVIKE 479
           +FGNAA V QNS L  RKP P  +   TAQSR   +Q TGI I  CRI A  DL PV + 
Sbjct: 412 IFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKEN 471

Query: 480 FPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAGANTSGR 539
           F ++LGRPW +Y+R V++++ I ++I   GW     D  ++  Y+ EY N G GAN + R
Sbjct: 472 FKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKR 531

Query: 540 VPW---SLVINEADAKTFTAERFIGGADWLPSTGFPYQLG 574
           V W     + N+ +A  FT   FI G+ WL STG P+ LG
Sbjct: 532 VTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSLG 531

BLAST of MELO3C021069 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 351.7 bits (901), Expect = 1.1e-96
Identity = 203/549 (36.98%), Postives = 307/549 (55.92%), Query Frame = 0

Query: 35  SCAITLYPQLCHSTISSIIGTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVS 94
           +C+ +LYP LC  T+    G S      +++ +S +  +   K +       T+++  + 
Sbjct: 78  TCSKSLYPNLCIDTLLDFPG-SLTADENELIHISFNATLQ--KFSKALYTSSTITYTQMP 137

Query: 95  KRDRIPLHDCVKSTDRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLD 154
            R R     C++  D ++  L +A+         +S      D+ T+LSSA+TN  TC D
Sbjct: 138 PRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDES----HSDVMTWLSSAMTNHDTCTD 197

Query: 155 GFQDDKTEKGVLR-VIENTHIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITS 214
           GF + + + G ++  +       +++ SN LA+                    F  K+  
Sbjct: 198 GFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAI--------------------FAGKVKD 257

Query: 215 ISS--QINDLKIVLFSNQEEGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVV 274
           +S    +N+ K++     EE               P W+  +D++LL + +S   AD+ V
Sbjct: 258 LSGVPVVNNRKLLGTEETEE--------------LPNWLKREDRELLGTPTSAIQADITV 317

Query: 275 AVDGSGNYKTVSEAVEAAPSKNSKRYIIKIKAGVY-RENVDVPSSKRNIMFWGDGRSNTI 334
           + DGSG +KT++EA++ AP  +S+R++I +KAG Y  EN+ V   K N+MF GDG+  T+
Sbjct: 318 SKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTV 377

Query: 335 ITAYRSHGSGWSTFNSATVVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCG 394
           IT  +S     +TF++AT  A G GF+ RD++FEN AGPA  QAVALRVG DH+  YRC 
Sbjct: 378 ITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCN 437

Query: 395 MLGYQDTLYVHSNRQFFVNCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSR 454
           ++GYQD LYVHSNRQFF  C + GTVDF+FGNAA ++Q+ ++  RKP   Q+  +TAQ+R
Sbjct: 438 IIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNR 497

Query: 455 TDLNQNTGIVIQKCRIKATSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWS 514
            D NQNTGI I  C++ AT DLE     +P++LGRPW+ Y+RVV M + + + ID +GW 
Sbjct: 498 KDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWL 557

Query: 515 TWNGD--IKKPYFAEYDNNGAGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPS 574
            WNG   +   Y+ EY N G G+    RV W    ++ +  +A  FT  +FI G+ WLPS
Sbjct: 558 EWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPS 585

BLAST of MELO3C021069 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 344.7 bits (883), Expect = 1.4e-94
Identity = 212/544 (38.97%), Postives = 313/544 (57.54%), Query Frame = 0

Query: 36  CAITLYPQLCHSTISSIIGTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSFNNVSK 95
           C+ T  P+ C   ++       + S  + +++S+ + +D A L        T+       
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAIL--AKTHAFTLGPKCRDT 93

Query: 96  RDRIPLHDCVKSTDRTIYELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDG 155
           R++    DC+K  D T+ ++++ ++     PN K       D +T+LS+A+TN  TC  G
Sbjct: 94  REKAAWEDCIKLYDLTVSKINETMD-----PNVKCSKL---DAQTWLSTALTNLDTCRAG 153

Query: 156 FQDDKTEKGVLRVIENTHIHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSIS 215
           F +      VL ++ N   + + L  N LA+ K                   P+  T   
Sbjct: 154 FLELGVTDIVLPLMSN---NVSNLLCNTLAINK------------------VPFNYT--- 213

Query: 216 SQINDLKIVLFSNQEEGENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDG 275
                        +++G             +P W+   D+KLL+SS+ +  A  VVA DG
Sbjct: 214 -----------PPEKDG-------------FPSWVKPGDRKLLQSSTPKDNA--VVAKDG 273

Query: 276 SGNYKTVSEAVEAAPSKNSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYR 335
           SGN+KT+ EA++AA    S R++I +K GVY EN+++   K+N+M  GDG   TIIT  +
Sbjct: 274 SGNFKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIITGSK 333

Query: 336 SHGSGWSTFNSATVVAVGDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQ 395
           S G G +TFNSATV AVGDGF+AR I+F NTAG +N QAVALR GSD S FY+C    YQ
Sbjct: 334 SVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQ 393

Query: 396 DTLYVHSNRQFFVNCVVAGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQ 455
           DTLYVHSNRQF+ +C V GTVDF+FGNAAAV+QN ++  R+P  ++ N +TAQ R+D NQ
Sbjct: 394 DTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQ 453

Query: 456 NTGIVIQKCRIKATSDLEPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD 515
           NTGI+I   R+ A SDL PV+    ++LGRPW +Y+R V M+T++ ++ID +GW  W+G+
Sbjct: 454 NTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGN 511

Query: 516 --IKKPYFAEYDNNGAGANTSGRVPW---SLVINEADAKTFTAERFIGGADWLPSTGFPY 575
             +K  ++AE+ N G GA+TSGRV W    ++ + ++A  FT   F+ G  W+PS+  P+
Sbjct: 514 FALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPF 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008458040.20.0e+0098.46PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo][more]
TYK30782.11.3e-30995.03pectinesterase-like [Cucumis melo var. makuwa][more]
KAA0026177.15.3e-30693.83pectinesterase-like [Cucumis melo var. makuwa][more]
XP_011659194.11.5e-29289.54pectinesterase [Cucumis sativus] >KGN44619.1 hypothetical protein Csa_015815 [Cu... [more]
XP_004139620.22.3e-27784.70pectinesterase [Cucumis sativus] >KGN44621.1 hypothetical protein Csa_015743 [Cu... [more]
Match NameE-valueIdentityDescription
Q431431.1e-15750.50Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... [more]
P839481.9e-15750.93Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
O048864.3e-15750.93Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
O490061.1e-14749.16Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
Q425342.4e-14448.05Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3C8610.0e+0098.46Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497582 PE=3 SV=1[more]
A0A5D3E4E86.4e-31095.03Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold343G00133... [more]
A0A5A7SNA22.6e-30693.83Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G001370... [more]
A0A0A0K6S67.3e-29389.54Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343850 PE=3 SV=1[more]
A0A0A0K9Y31.1e-27784.70Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343870 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14310.17.5e-14949.16pectin methylesterase 3 [more]
AT1G53830.11.7e-14548.05pectin methylesterase 2 [more]
AT4G02300.11.1e-9639.62Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G53370.11.1e-9636.98pectin methylesterase PCR fragment F [more]
AT2G45220.11.4e-9438.97Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 27..186
e-value: 6.3E-29
score: 112.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 6..191
e-value: 4.8E-23
score: 79.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 32..186
e-value: 1.0E-23
score: 84.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 262..574
e-value: 3.4E-118
score: 396.7
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 268..559
e-value: 2.2E-125
score: 417.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 28..192
e-value: 1.8E-28
score: 101.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 30..191
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 27..573
NoneNo IPR availablePANTHERPTHR31707:SF3PECTINESTERASE/PECTINESTERASE INHIBITOR 3coord: 27..573
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 34..192
e-value: 3.70554E-35
score: 127.554
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 412..421
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 263..573

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C021069.1MELO3C021069.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity