MELO3C020900 (gene) Melon (DHL92) v4

Overview


Name	MELO3C020900
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	EF hand calcium-binding family protein
Location	chr11: 3083677 .. 3085041 (-)
RNA-Seq Expression	MELO3C020900
Synteny	MELO3C020900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGGAGGGATATAAATAGAGGGTTGATGGTTATTCATTTTTTTGTGATAATTGGCATAGGAAGAGATGACAAGCTTATTGAAGTTATTCAGAAGCTGTTCTGGAAAGCGACCATCCAAAAACACTTCCTTTTCCATACAAATCAAAGAAAGGTCCAATTCTTCCTCAACCTCTTCAAAGCACTTCCGCCCAAACTTGGAGGAGATCAAATGGGTATGAACAATTCCTTTTTTTTCTTTTTTCTTCTTCTTTCTTTACATGAATTTATGGTGTCTACAGACTTATATAATGTTCATAAGAAATAACAAAGTTACTGAGAAAGTTATGAACTAGAACTCTCTATAACATCTTGTGCTACGTTCATAGTAATGATCTCTTTTGGATTTAATAGGCGTTTGAGAAGTTTGACTCGAACAAGGATGGAAAGATCTCATTTGAGGAGTACAAGGAAGCTCACCGAGGCTTGGCTGGAAGCAAAGAAATCACAGACGCAGAGGCAGAGAAGTCATTCAAGCTTGTGGATGCAGATGGCGATGGATTCGTGGATTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCAGCGGGGAAGTGAAGGTGGGTGACATCGAAAGTGCCTTCAAAGTGTACGATTCCAATGGAGATGGGAAGATAAGCGCAGAGGAGGTGATGGGAATCATGAAGATATTGGGAGAGAACACCACCTTAAATGCTTGCAAGCAAATGGTGAAGGGTGTGGACATGGATGGGGATGGCTTCATTGATATTCAAGAGTTTTCTAAGCTCATGGATACGTACTCGAGTAAGTTTCAAAAGTAAAGAAGCTAAAACTATAGTGTTCCGTTTGGTAACCATTTCATTTATTGTTTTAGAAGGATCATTTACATTTTTCTCAAAGTGCTATAGTTAATTTCTTACTTAATTTTCAAAAAGAAAAAAAATAAATAATAAAATGGTTAACAAACGAGACCTACACGTACCTCTAGTTACATGAATTTACATGAATGGAGGTGGAAAACAAAAATTCTAAGCTATGTTTGAAGTTTGTATCCAATAATTAGGAGGATGGTTATATTATATTGTGGCAATGTTGTTGATTAATGTGCCACTACTAGTCATTATGCAAAGTGTAGATTTGTGATGTAATTTTTGTTAAGTTGTCAACATCTTCAAACGGTGAAGGGTTCGATGTTGATAGTGTGAATTGAAAATTTCTATTATGTAAGAAAATTGATGGCAAGATTACTTTTATTGATATTTATGTCAAAATTTACATAACTATTGCCTATATTTTTATAATGGGTGAATATAGTATATAACTTCGAGATTTTTAGAATATTTTCGAATATAACATAATAAACTAAAATA

mRNA sequence

AAGGAGGGATATAAATAGAGGGTTGATGGTTATTCATTTTTTTGTGATAATTGGCATAGGAAGAGATGACAAGCTTATTGAAGTTATTCAGAAGCTGTTCTGGAAAGCGACCATCCAAAAACACTTCCTTTTCCATACAAATCAAAGAAAGGTCCAATTCTTCCTCAACCTCTTCAAAGCACTTCCGCCCAAACTTGGAGGAGATCAAATGGGCGTTTGAGAAGTTTGACTCGAACAAGGATGGAAAGATCTCATTTGAGGAGTACAAGGAAGCTCACCGAGGCTTGGCTGGAAGCAAAGAAATCACAGACGCAGAGGCAGAGAAGTCATTCAAGCTTGTGGATGCAGATGGCGATGGATTCGTGGATTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCAGCGGGGAAGTGAAGGTGGGTGACATCGAAAGTGCCTTCAAAGTGTACGATTCCAATGGAGATGGGAAGATAAGCGCAGAGGAGGTGATGGGAATCATGAAGATATTGGGAGAGAACACCACCTTAAATGCTTGCAAGCAAATGGTGAAGGGTGTGGACATGGATGGGGATGGCTTCATTGATATTCAAGAGTTTTCTAAGCTCATGGATACGTACTCGAGTAAGTTTCAAAAGTAAAGAAGCTAAAACTATAGTGTTCCGTTTGGTAACCATTTCATTTATTGTTTTAGAAGGATCATTTACATTTTTCTCAAAGTGCTATAGTTAATTTCTTACTTAATTTTCAAAAAGAAAAAAAATAAATAATAAAATGGTTAACAAACGAGACCTACACGTACCTCTAGTTACATGAATTTACATGAATGGAGGTGGAAAACAAAAATTCTAAGCTATGTTTGAAGTTTGTATCCAATAATTAGGAGGATGGTTATATTATATTGTGGCAATGTTGTTGATTAATGTGCCACTACTAGTCATTATGCAAAGTGTAGATTTGTGATGTAATTTTTGTTAAGTTGTCAACATCTTCAAACGGTGAAGGGTTCGATGTTGATAGTGTGAATTGAAAATTTCTATTATGTAAGAAAATTGATGGCAAGATTACTTTTATTGATATTTATGTCAAAATTTACATAACTATTGCCTATATTTTTATAATGGGTGAATATAGTATATAACTTCGAGATTTTTAGAATATTTTCGAATATAACATAATAAACTAAAATA

Coding sequence (CDS)

ATGACAAGCTTATTGAAGTTATTCAGAAGCTGTTCTGGAAAGCGACCATCCAAAAACACTTCCTTTTCCATACAAATCAAAGAAAGGTCCAATTCTTCCTCAACCTCTTCAAAGCACTTCCGCCCAAACTTGGAGGAGATCAAATGGGCGTTTGAGAAGTTTGACTCGAACAAGGATGGAAAGATCTCATTTGAGGAGTACAAGGAAGCTCACCGAGGCTTGGCTGGAAGCAAAGAAATCACAGACGCAGAGGCAGAGAAGTCATTCAAGCTTGTGGATGCAGATGGCGATGGATTCGTGGATTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCAGCGGGGAAGTGAAGGTGGGTGACATCGAAAGTGCCTTCAAAGTGTACGATTCCAATGGAGATGGGAAGATAAGCGCAGAGGAGGTGATGGGAATCATGAAGATATTGGGAGAGAACACCACCTTAAATGCTTGCAAGCAAATGGTGAAGGGTGTGGACATGGATGGGGATGGCTTCATTGATATTCAAGAGTTTTCTAAGCTCATGGATACGTACTCGAGTAAGTTTCAAAAGTAA

Protein sequence

MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKLMDTYSSKFQK

Homology

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BLAST of MELO3C020900 vs. NCBI nr
Match: XP_008457835.1 (PREDICTED: probable calcium-binding protein CML32 [Cucumis melo] >KAA0045818.1 putative calcium-binding protein CML32 [Cucumis melo var. makuwa] >TYJ99463.1 putative calcium-binding protein CML32 [Cucumis melo var. makuwa])

HSP 1 Score: 367.1 bits (941), Expect = 9.3e-98
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0


Query: 1   MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG 60
           MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL 180

Query: 181 MDTYSSKFQK 191
           MDTYSSKFQK
Sbjct: 181 MDTYSSKFQK 190

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BLAST of MELO3C020900 vs. NCBI nr
Match: XP_004148036.1 (probable calcium-binding protein CML32 [Cucumis sativus] >KGN62012.1 hypothetical protein Csa_006079 [Cucumis sativus])

HSP 1 Score: 349.7 bits (896), Expect = 1.5e-92
Identity = 181/189 (95.77%), Postives = 183/189 (96.83%), Query Frame = 0


Query: 1   MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG 60
           M+SLLKLFRSCSGKRPSKNTSFSI IKERSNSSS SSKHFRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVD DGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTL ACKQMVKGVDMDGDGFID+QEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180

Query: 181 MDTYSSKFQ 190
           M  YSSKFQ
Sbjct: 181 MGKYSSKFQ 189

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BLAST of MELO3C020900 vs. NCBI nr
Match: XP_038901754.1 (probable calcium-binding protein CML32 [Benincasa hispida])

HSP 1 Score: 298.9 bits (764), Expect = 3.1e-77
Identity = 154/187 (82.35%), Postives = 165/187 (88.24%), Query Frame = 0


Query: 3   SLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDGKI 62
           S+L LF SC+ KR  KN   S QIK+RSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDGKI
Sbjct: 7   SVLSLFTSCTRKRQPKNIPSSTQIKQRSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDGKI 66

Query: 63  SFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGDIE 122
           SFEEY+ AHRG+AGSKEI+D EA KSFK VD DGDGFVDLKEF+ELYTMS GEV+VGDI+
Sbjct: 67  SFEEYQAAHRGMAGSKEISDEEAAKSFKFVDVDGDGFVDLKEFLELYTMSGGEVRVGDIK 126

Query: 123 SAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKLMD 182
           SAF+VYDSNGDGKISAEEVM IM  LGE TTL ACKQMVKGVD+DGDGFIDI EFSKLM 
Sbjct: 127 SAFQVYDSNGDGKISAEEVMAIMNTLGERTTLKACKQMVKGVDIDGDGFIDIDEFSKLMG 186

Query: 183 TYSSKFQ 190
           TYSSKFQ
Sbjct: 187 TYSSKFQ 193

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BLAST of MELO3C020900 vs. NCBI nr
Match: XP_023532624.1 (calmodulin-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 270.0 bits (689), Expect = 1.5e-68
Identity = 144/188 (76.60%), Postives = 158/188 (84.04%), Query Frame = 0


Query: 3   SLLKLFRSCSGKRPSKNTSFSIQIKERSNS---SSTSSKHFRPNLEEIKWAFEKFDSNKD 62
           S+L LF SCS KR  K    S Q K+RS S   SSTSSKH R NLEEIKWAF+KFD+NKD
Sbjct: 7   SVLSLFTSCSPKRQLKQIPSSTQPKQRSKSISNSSTSSKHVRVNLEEIKWAFDKFDTNKD 66

Query: 63  GKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVG 122
           GKISFEEYK AH  ++GSKEI+DAEA KSF+ VDADGDGFV+LKEFVELYTMS GEVK+G
Sbjct: 67  GKISFEEYKAAHGAMSGSKEISDAEAAKSFEFVDADGDGFVELKEFVELYTMSGGEVKMG 126

Query: 123 DIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSK 182
           D++SAFKVYDSNGDGKISAEEVM IMKILGE+TTL AC+QMVKGVD DGDGFIDI EFS 
Sbjct: 127 DVQSAFKVYDSNGDGKISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFIDIDEFSS 186

Query: 183 LMDTYSSK 188
           LM TYS K
Sbjct: 187 LMGTYSFK 194

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BLAST of MELO3C020900 vs. NCBI nr
Match: KAG6605248.1 (Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 269.2 bits (687), Expect = 2.6e-68
Identity = 144/188 (76.60%), Postives = 158/188 (84.04%), Query Frame = 0


Query: 3   SLLKLFRSCSGKRPSKNTSFSIQIKERSNS---SSTSSKHFRPNLEEIKWAFEKFDSNKD 62
           S+L +F SCS KR  K    S Q K+RS S   SS SSKH R NLEEIKWAF+KFDSNKD
Sbjct: 7   SVLSIFTSCSPKRQLKQIPSSTQPKQRSKSISDSSASSKHVRVNLEEIKWAFDKFDSNKD 66

Query: 63  GKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVG 122
           GKISFEEYK AH  ++GSKEI+DAEA KSFKLVDADGDGFV+LKEFVELYTMS GEVK+G
Sbjct: 67  GKISFEEYKAAHGAMSGSKEISDAEAAKSFKLVDADGDGFVELKEFVELYTMSGGEVKMG 126

Query: 123 DIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSK 182
           DI+SAFKVYDSNGDGKISAEEVM IMKILGE+TTL AC+QMVKGVD DGDGFID+ EFS 
Sbjct: 127 DIQSAFKVYDSNGDGKISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFIDMDEFSS 186

Query: 183 LMDTYSSK 188
           LM +YS K
Sbjct: 187 LMGSYSFK 194

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BLAST of MELO3C020900 vs. ExPASy Swiss-Prot
Match: Q9ZQE6 (Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 9.5e-29
Identity = 75/178 (42.13%), Postives = 109/178 (61.24%), Query Frame = 0


Query: 6   KLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDGKISFE 65
           KL ++   ++PS+   FS   +    SS       +P++ E++  F +FD +KDGKIS  
Sbjct: 12  KLSKNKMLRKPSR--MFSRDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKDGKISQT 71

Query: 66  EYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGDIESAF 125
           EYK   R L   + I D    K FK VD DGDGF+D +EF++ Y  S G ++  DI ++F
Sbjct: 72  EYKVVLRALGQERAIED--VPKIFKAVDLDGDGFIDFREFIDAYKRSGG-IRSSDIRNSF 131

Query: 126 KVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKLMDT 184
             +D NGDGKISAEEVM ++  LGE  +L  C +MV+ VD DGDG ++++EF K+M +
Sbjct: 132 WTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKMMSS 184

[Close]

BLAST of MELO3C020900 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 2.4e-24
Identity = 61/144 (42.36%), Postives = 91/144 (63.19%), Query Frame = 0


Query: 43  NLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDL 102
           ++++IK  F++FD N DGKIS +E KE  R L  S   +  E     K  D DG+GF+DL
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRAL--SPTASPEETVTMMKQFDLDGNGFIDL 73

Query: 103 KEFVELYTMSSG-----EVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNAC 162
            EFV L+ +  G        V D++ AF++YD +G+G+ISA+E+  +MK LGE  ++  C
Sbjct: 74  DEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDC 133

Query: 163 KQMVKGVDMDGDGFIDIQEFSKLM 182
           K+M+  VD+DGDG ++  EF K+M
Sbjct: 134 KKMISKVDIDGDGCVNFDEFKKMM 155

[Close]

BLAST of MELO3C020900 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.2e-23
Identity = 61/152 (40.13%), Postives = 93/152 (61.18%), Query Frame = 0


Query: 33  SSTSSKHFRPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLV 92
           S   S++   ++E+IK  F++FD N DGKIS +E K+    L  S   +  E +   K  
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGAL--SPNASQEETKAMMKEF 61

Query: 93  DADGDGFVDLKEFVELYTM---SSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILG 152
           D DG+GF+DL EFV L+ +   SS    + D++ AF +YD + +G+ISA E+  +MK LG
Sbjct: 62  DLDGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG 121

Query: 153 ENTTLNACKQMVKGVDMDGDGFIDIQEFSKLM 182
           E  ++  C++M+  VD DGDG +D +EF K+M
Sbjct: 122 EKCSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of MELO3C020900 vs. ExPASy Swiss-Prot
Match: Q9LF55 (Probable calcium-binding protein CML32 OS=Arabidopsis thaliana OX=3702 GN=CML32 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.7e-22
Identity = 62/143 (43.36%), Postives = 85/143 (59.44%), Query Frame = 0


Query: 51  FEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYT 110
           FE+ D NKDGKIS++E+ EA R  A S  IT  E +  F+ +D DGD  +D+ E+     
Sbjct: 7   FERVDKNKDGKISWDEFAEAIR--AFSPSITSEEIDNMFREIDVDGDNQIDVAEYASC-L 66

Query: 111 MSSGEVKVGD----IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDM 170
           M  GE    D    ++ AF +YD +GDGKISA E+  ++K LGE  T+  C  MV+ VD 
Sbjct: 67  MLGGEGNKEDEDIVMKEAFDLYDIDGDGKISASEIHVVLKRLGEKQTIAECIAMVRAVDA 126

Query: 171 DGDGFIDIQEFSKLMDTYSSKFQ 190
           DGDGF+  +EF  +M   + K Q
Sbjct: 127 DGDGFVSFEEFKTMMSCNNKKLQ 146

[Close]

BLAST of MELO3C020900 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.5e-21
Identity = 60/149 (40.27%), Postives = 94/149 (63.09%), Query Frame = 0


Query: 41  RPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFV 100
           +  + E++  F+KFD N DGKIS +E       L    E+ + E EK+   +D  GDG++
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSL--GHEVPEEELEKAITEIDRKGDGYI 91

Query: 101 DLKEFVELYT--MSSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACK 160
           + +EFVEL T  M   +V + +++ AF VYD +G+G ISAEE+  +++ LG+  ++  C+
Sbjct: 92  NFEEFVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECR 151

Query: 161 QMVKGVDMDGDGFIDIQEFSKLMDTYSSK 188
           +M+ GVD DGDG ID +EF K+M T  S+
Sbjct: 152 KMIGGVDKDGDGTIDFEEF-KIMMTMGSR 176

[Close]

BLAST of MELO3C020900 vs. ExPASy TrEMBL
Match: A0A5D3BJJ3 (Putative calcium-binding protein CML32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00260 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 4.5e-98
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0


Query: 1   MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG 60
           MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL 180

Query: 181 MDTYSSKFQK 191
           MDTYSSKFQK
Sbjct: 181 MDTYSSKFQK 190

[Close]

BLAST of MELO3C020900 vs. ExPASy TrEMBL
Match: A0A1S3C6F1 (probable calcium-binding protein CML32 OS=Cucumis melo OX=3656 GN=LOC103497422 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 4.5e-98
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0


Query: 1   MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG 60
           MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL 180

Query: 181 MDTYSSKFQK 191
           MDTYSSKFQK
Sbjct: 181 MDTYSSKFQK 190

[Close]

BLAST of MELO3C020900 vs. ExPASy TrEMBL
Match: A0A0A0LMP3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G286450 PE=4 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 7.4e-93
Identity = 181/189 (95.77%), Postives = 183/189 (96.83%), Query Frame = 0


Query: 1   MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG 60
           M+SLLKLFRSCSGKRPSKNTSFSI IKERSNSSS SSKHFRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVD DGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTL ACKQMVKGVDMDGDGFID+QEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180

Query: 181 MDTYSSKFQ 190
           M  YSSKFQ
Sbjct: 181 MGKYSSKFQ 189

[Close]

BLAST of MELO3C020900 vs. ExPASy TrEMBL
Match: A0A6J1G5E9 (calmodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111450966 PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.2e-68
Identity = 144/188 (76.60%), Postives = 157/188 (83.51%), Query Frame = 0


Query: 3   SLLKLFRSCSGKRPSKNTSFSIQIKERSNS---SSTSSKHFRPNLEEIKWAFEKFDSNKD 62
           S+L LF SCS KR  K    S Q K+RS S   SS SSKH R NLEEIKW F+KFD+NKD
Sbjct: 7   SVLSLFTSCSPKRQLKQIPSSTQPKQRSKSISDSSASSKHVRVNLEEIKWVFDKFDTNKD 66

Query: 63  GKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVG 122
           GKISFEEYK AH  ++GSKEI+DAEA KSFKLVDADGDGFV+LKEFVELYTMS GEVK+G
Sbjct: 67  GKISFEEYKAAHGAMSGSKEISDAEAAKSFKLVDADGDGFVELKEFVELYTMSGGEVKMG 126

Query: 123 DIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSK 182
           DI+SAFKVYDSNGDGKISAEEVM IMKILGE+TTL AC+QMVKGVD DGDGFIDI EFS 
Sbjct: 127 DIQSAFKVYDSNGDGKISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFIDIDEFSS 186

Query: 183 LMDTYSSK 188
           LM +YS K
Sbjct: 187 LMGSYSFK 194

[Close]

BLAST of MELO3C020900 vs. ExPASy TrEMBL
Match: A0A6J1L0C4 (calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111499885 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.8e-67
Identity = 141/188 (75.00%), Postives = 156/188 (82.98%), Query Frame = 0


Query: 3   SLLKLFRSCSGKRPSKNTSFSIQIKERSNS---SSTSSKHFRPNLEEIKWAFEKFDSNKD 62
           S+L LF SCS KR  K    S Q K+R  S   SS SSKH + NLEEIKWAFEKFD+NKD
Sbjct: 7   SVLSLFTSCSPKRQLKQIPSSTQPKQRFKSISDSSASSKHVQVNLEEIKWAFEKFDTNKD 66

Query: 63  GKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVG 122
           GKISFEEYK AHR ++GS+EI+D EA KSFK VDADGDGFV+LKEFVELYTMS GEVK+G
Sbjct: 67  GKISFEEYKAAHRAMSGSQEISDVEAAKSFKFVDADGDGFVELKEFVELYTMSGGEVKMG 126

Query: 123 DIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSK 182
           D++SAFKVYDSNGDG+ISAEEVM IMKILGE+TTL AC+QMVKGVD DGDGFIDI EFS 
Sbjct: 127 DVQSAFKVYDSNGDGRISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFIDIDEFSS 186

Query: 183 LMDTYSSK 188
           LM TYS K
Sbjct: 187 LMGTYSFK 194

[Close]

BLAST of MELO3C020900 vs. TAIR 10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 128.3 bits (321), Expect = 6.8e-30
Identity = 75/178 (42.13%), Postives = 109/178 (61.24%), Query Frame = 0


Query: 6   KLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDGKISFE 65
           KL ++   ++PS+   FS   +    SS       +P++ E++  F +FD +KDGKIS  
Sbjct: 12  KLSKNKMLRKPSR--MFSRDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKDGKISQT 71

Query: 66  EYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGDIESAF 125
           EYK   R L   + I D    K FK VD DGDGF+D +EF++ Y  S G ++  DI ++F
Sbjct: 72  EYKVVLRALGQERAIED--VPKIFKAVDLDGDGFIDFREFIDAYKRSGG-IRSSDIRNSF 131

Query: 126 KVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKLMDT 184
             +D NGDGKISAEEVM ++  LGE  +L  C +MV+ VD DGDG ++++EF K+M +
Sbjct: 132 WTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKMMSS 184

[Close]

BLAST of MELO3C020900 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 113.6 bits (283), Expect = 1.7e-25
Identity = 61/144 (42.36%), Postives = 91/144 (63.19%), Query Frame = 0


Query: 43  NLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDL 102
           ++++IK  F++FD N DGKIS +E KE  R L  S   +  E     K  D DG+GF+DL
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRAL--SPTASPEETVTMMKQFDLDGNGFIDL 73

Query: 103 KEFVELYTMSSG-----EVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNAC 162
            EFV L+ +  G        V D++ AF++YD +G+G+ISA+E+  +MK LGE  ++  C
Sbjct: 74  DEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDC 133

Query: 163 KQMVKGVDMDGDGFIDIQEFSKLM 182
           K+M+  VD+DGDG ++  EF K+M
Sbjct: 134 KKMISKVDIDGDGCVNFDEFKKMM 155

[Close]

BLAST of MELO3C020900 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 111.3 bits (277), Expect = 8.6e-25
Identity = 61/152 (40.13%), Postives = 93/152 (61.18%), Query Frame = 0


Query: 33  SSTSSKHFRPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLV 92
           S   S++   ++E+IK  F++FD N DGKIS +E K+    L  S   +  E +   K  
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGAL--SPNASQEETKAMMKEF 61

Query: 93  DADGDGFVDLKEFVELYTM---SSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILG 152
           D DG+GF+DL EFV L+ +   SS    + D++ AF +YD + +G+ISA E+  +MK LG
Sbjct: 62  DLDGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG 121

Query: 153 ENTTLNACKQMVKGVDMDGDGFIDIQEFSKLM 182
           E  ++  C++M+  VD DGDG +D +EF K+M
Sbjct: 122 EKCSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of MELO3C020900 vs. TAIR 10
Match: AT5G17470.1 (EF hand calcium-binding protein family )

HSP 1 Score: 107.5 bits (267), Expect = 1.2e-23
Identity = 62/143 (43.36%), Postives = 85/143 (59.44%), Query Frame = 0


Query: 51  FEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYT 110
           FE+ D NKDGKIS++E+ EA R  A S  IT  E +  F+ +D DGD  +D+ E+     
Sbjct: 7   FERVDKNKDGKISWDEFAEAIR--AFSPSITSEEIDNMFREIDVDGDNQIDVAEYASC-L 66

Query: 111 MSSGEVKVGD----IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDM 170
           M  GE    D    ++ AF +YD +GDGKISA E+  ++K LGE  T+  C  MV+ VD 
Sbjct: 67  MLGGEGNKEDEDIVMKEAFDLYDIDGDGKISASEIHVVLKRLGEKQTIAECIAMVRAVDA 126

Query: 171 DGDGFIDIQEFSKLMDTYSSKFQ 190
           DGDGF+  +EF  +M   + K Q
Sbjct: 127 DGDGFVSFEEFKTMMSCNNKKLQ 146

[Close]

BLAST of MELO3C020900 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 104.4 bits (259), Expect = 1.0e-22
Identity = 60/149 (40.27%), Postives = 94/149 (63.09%), Query Frame = 0


Query: 41  RPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFV 100
           +  + E++  F+KFD N DGKIS +E       L    E+ + E EK+   +D  GDG++
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSL--GHEVPEEELEKAITEIDRKGDGYI 91

Query: 101 DLKEFVELYT--MSSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACK 160
           + +EFVEL T  M   +V + +++ AF VYD +G+G ISAEE+  +++ LG+  ++  C+
Sbjct: 92  NFEEFVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECR 151

Query: 161 QMVKGVDMDGDGFIDIQEFSKLMDTYSSK 188
           +M+ GVD DGDG ID +EF K+M T  S+
Sbjct: 152 KMIGGVDKDGDGTIDFEEF-KIMMTMGSR 176

The following BLAST results are available for this feature:

BLAST of MELO3C020900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008457835.1	9.3e-98	100.00	PREDICTED: probable calcium-binding protein CML32 [Cucumis melo] >KAA0045818.1 p...	[more]
XP_004148036.1	1.5e-92	95.77	probable calcium-binding protein CML32 [Cucumis sativus] >KGN62012.1 hypothetica...	[more]
XP_038901754.1	3.1e-77	82.35	probable calcium-binding protein CML32 [Benincasa hispida]	[more]
XP_023532624.1	1.5e-68	76.60	calmodulin-like protein 1 [Cucurbita pepo subsp. pepo]	[more]
KAG6605248.1	2.6e-68	76.60	Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MELO3C020900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQE6	9.5e-29	42.13	Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1	[more]
P25070	2.4e-24	42.36	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q9C8Y1	1.2e-23	40.13	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
Q9LF55	1.7e-22	43.36	Probable calcium-binding protein CML32 OS=Arabidopsis thaliana OX=3702 GN=CML32 ...	[more]
Q9FYK2	1.5e-21	40.27	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]

BLAST of MELO3C020900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BJJ3	4.5e-98	100.00	Putative calcium-binding protein CML32 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C6F1	4.5e-98	100.00	probable calcium-binding protein CML32 OS=Cucumis melo OX=3656 GN=LOC103497422 P...	[more]
A0A0A0LMP3	7.4e-93	95.77	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G286450 PE=4 SV=1	[more]
A0A6J1G5E9	2.2e-68	76.60	calmodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111450966 PE=4 SV=...	[more]
A0A6J1L0C4	1.8e-67	75.00	calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111499885 PE=4 SV=1	[more]

BLAST of MELO3C020900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G15680.1	6.8e-30	42.13	Calcium-binding EF-hand family protein	[more]
AT5G37770.1	1.7e-25	42.36	EF hand calcium-binding protein family	[more]
AT1G66400.1	8.6e-25	40.13	calmodulin like 23	[more]
AT5G17470.1	1.2e-23	43.36	EF hand calcium-binding protein family	[more]
AT1G24620.1	1.0e-22	40.27	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 120..148 e-value: 1.8E-5 score: 34.2 coord: 156..184 e-value: 0.82 score: 18.5 coord: 84..112 e-value: 9.3E-5 score: 31.8 coord: 46..74 e-value: 1.7E-6 score: 37.5
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 45..109 e-value: 7.6E-15 score: 55.1 coord: 121..181 e-value: 5.1E-9 score: 36.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 116..151 score: 15.043417
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 80..115 score: 13.760229
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 152..187 score: 11.695971
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 42..77 score: 15.712908
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 120..181 e-value: 2.00216E-16 score: 68.3433
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 46..108 e-value: 1.14924E-13 score: 61.0245
None	No IPR available	GENE3D	1.10.238.10		coord: 114..188 e-value: 1.8E-18 score: 69.2
None	No IPR available	GENE3D	1.10.238.10		coord: 3..113 e-value: 1.2E-19 score: 72.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 14..39
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 6..184
None	No IPR available	PANTHER	PTHR10891:SF685	CALCIUM-BINDING PROTEIN CML34-RELATED	coord: 6..184
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 93..105
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 129..141
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 55..67
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 165..177
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 44..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C020900.1	MELO3C020900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding