MELO3C020579 (gene) Melon (DHL92) v4

BLAST of MELO3C020579 vs. ExPASy Swiss-Prot
Match: Q9ZW34 (Cytosolic enolase 3 OS=Arabidopsis thaliana OX=3702 GN=ENO3 PE=1 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 2.1e-225
Identity = 383/472 (81.14%), Postives = 429/472 (90.89%), Query Frame = 0

Query: 75  MSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKARQILDSRG 134
           MSVQEYLDK+MLSRKIE+AVNAAVRAKT DPVLFI+NH+KKA+ SVITK+KARQILDSRG
Sbjct: 1   MSVQEYLDKHMLSRKIEDAVNAAVRAKTSDPVLFIANHLKKAVSSVITKVKARQILDSRG 60

Query: 135 IPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKNINEKISEA 194
           IPTVEVDL+TNKGVF ASVPSGD +G YEA+ELRDGDKG YLGNSV KAVKNINEKISEA
Sbjct: 61  IPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSVAKAVKNINEKISEA 120

Query: 195 LIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPLYKHIADLA 254
           LIG DP  Q +IDQAM DLD TEKK ELGANAILAVSIA C+AGAAEKEVPL KH++DL+
Sbjct: 121 LIGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAAEKEVPLCKHLSDLS 180

Query: 255 GKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFHHLKAVITE 314
           G+ N++LPVPAFTVLSGGKHA N  AIQEIMILPIGAS+FEEAL+ GSET+HHLKAVI+E
Sbjct: 181 GRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQWGSETYHHLKAVISE 240

Query: 315 KHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATNFCMGTKYD 374
           K+G   CN GEDGG AP+ISS KE L+LV+EAINR GYN++IKIAID+AATNFC+GTKYD
Sbjct: 241 KNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAIDIAATNFCLGTKYD 300

Query: 375 LDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSSLGICQVVG 434
           LD K+PNKS QNFKS +DMIDMYKE+C DYPIVSIEDPFD+EDW+HTK+FSSLGICQVVG
Sbjct: 301 LDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWEHTKYFSSLGICQVVG 360

Query: 435 GDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVASHRCGETD 494
            DLLMSN KR+ERAI E +CNALLLKVNQIGTVTEAIEVVK+A+DAQW VV SHRCGET+
Sbjct: 361 DDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARDAQWGVVTSHRCGETE 420

Query: 495 DTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 547
           D+F++DLSVGL+T  IKAGAPCRGER  KYNQLLRIEEELGDQA+YAGEDW+
Sbjct: 421 DSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAVYAGEDWK 472

BLAST of MELO3C020579 vs. ExPASy Swiss-Prot
Match: Q27527 (Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3)

HSP 1 Score: 505.8 bits (1301), Expect = 6.3e-142
Identity = 254/428 (59.35%), Postives = 316/428 (73.83%), Query Frame = 0

Query: 121 ITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSV 180
           ITKI ARQI DSRG PTVEVDL+T KGVF A+VPSG   G++EA+ELRDGDK  +LG  V
Sbjct: 3   ITKIHARQIYDSRGNPTVEVDLFTEKGVFRAAVPSGASTGVHEALELRDGDKAVHLGKGV 62

Query: 181 TKAVKNINEKISEALI--GRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAG 240
            KAV NINEKI+ ALI  G D T Q +ID  M  LD +E KG LGANAIL VS+AV +AG
Sbjct: 63  LKAVSNINEKIAPALIAKGFDVTAQKDIDDFMMALDGSENKGNLGANAILGVSLAVAKAG 122

Query: 241 AAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEAL 300
           A  K +PLYK+IA+LAG   ++LPVPAF V++GG HAGN LA+QE MILP+GAS F EA+
Sbjct: 123 AVHKGLPLYKYIAELAGTGKVVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFAEAM 182

Query: 301 KMGSETFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKI 360
           +MGSE +HHLKA I +++G      G++GGFAPNI   KE LDL+  AI++ GY  +I I
Sbjct: 183 RMGSEVYHHLKAEIKKRYGLDATAVGDEGGFAPNIQDNKEGLDLLNTAIDKAGYTGKISI 242

Query: 361 AIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDW 420
            +DVAA+ F    KYDLDFK P      + SG+ + ++Y+    +YP+VSIED FD++DW
Sbjct: 243 GMDVAASEFFKDGKYDLDFKNPASDSSKWLSGEQLTELYQSFIKEYPVVSIEDAFDQDDW 302

Query: 421 DHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAK 480
           D+   F      Q+VG DL ++N KRI+ AID+ +CN LLLKVNQIG+VTE+IE  KL++
Sbjct: 303 DNWGKFHGATSIQLVGDDLTVTNPKRIQTAIDKKSCNCLLLKVNQIGSVTESIEAAKLSR 362

Query: 481 DAQWAVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQA 540
              W V+ SHR GET+DTF+ADL VGL+T QIK GAPCR ERLAKYNQLLRIEEELG  A
Sbjct: 363 ANGWGVMVSHRSGETEDTFIADLVVGLATGQIKTGAPCRSERLAKYNQLLRIEEELGADA 422

Query: 541 IYAGEDWR 547
           +YAG ++R
Sbjct: 423 VYAGHNFR 430

BLAST of MELO3C020579 vs. ExPASy Swiss-Prot
Match: P09104 (Gamma-enolase OS=Homo sapiens OX=9606 GN=ENO2 PE=1 SV=3)

HSP 1 Score: 505.4 bits (1300), Expect = 8.3e-142
Identity = 253/428 (59.11%), Postives = 322/428 (75.23%), Query Frame = 0

Query: 121 ITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSV 180
           I KI AR+ILDSRG PTVEVDLYT KG+F A+VPSG   G+YEA+ELRDGDK  YLG  V
Sbjct: 3   IEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGV 62

Query: 181 TKAVKNINEKISEALI--GRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAG 240
            KAV +IN  I+ ALI  G    +Q ++D  M +LD TE K + GANAIL VS+AVC+AG
Sbjct: 63  LKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAG 122

Query: 241 AAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEAL 300
           AAE+E+PLY+HIA LAG ++LILPVPAF V++GG HAGN LA+QE MILP+GA  F +A+
Sbjct: 123 AAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAM 182

Query: 301 KMGSETFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKI 360
           ++G+E +H LK VI +K+G    N G++GGFAPNI    EAL+LV+EAI++ GY E+I I
Sbjct: 183 RLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKIVI 242

Query: 361 AIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDW 420
            +DVAA+ F    KYDLDFK+P      + +G  +  +Y++   DYP+VSIEDPFD++DW
Sbjct: 243 GMDVAASEFYRDGKYDLDFKSPT-DPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDDW 302

Query: 421 DHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAK 480
                F++    Q+VG DL ++N KRIERA++E  CN LLLKVNQIG+VTEAI+  KLA+
Sbjct: 303 AAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQ 362

Query: 481 DAQWAVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQA 540
           +  W V+ SHR GET+DTF+ADL VGL T QIK GAPCR ERLAKYNQL+RIEEELGD+A
Sbjct: 363 ENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEA 422

Query: 541 IYAGEDWR 547
            +AG ++R
Sbjct: 423 RFAGHNFR 429

BLAST of MELO3C020579 vs. ExPASy Swiss-Prot
Match: P17183 (Gamma-enolase OS=Mus musculus OX=10090 GN=Eno2 PE=1 SV=2)

HSP 1 Score: 501.5 bits (1290), Expect = 1.2e-140
Identity = 250/428 (58.41%), Postives = 323/428 (75.47%), Query Frame = 0

Query: 121 ITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSV 180
           I KI AR+ILDSRG PTVEVDLYT KG+F A+VPSG   G+YEA+ELRDGDK  YLG  V
Sbjct: 3   IEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGV 62

Query: 181 TKAVKNINEKISEALI--GRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAG 240
            KAV +IN +I+ ALI  G    +Q ++D  M +LD TE K + GANAIL VS+AVC+AG
Sbjct: 63  LKAVDHINSRIAPALISSGISVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAG 122

Query: 241 AAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEAL 300
           AAE+++PLY+HIA LAG ++LILPVPAF V++GG HAGN LA+QE MILP+GA  F +A+
Sbjct: 123 AAERDLPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAM 182

Query: 301 KMGSETFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKI 360
           ++G+E +H LK VI +K+G    N G++GGFAPNI    EAL+LV+EAI++ GY E++ I
Sbjct: 183 RLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKMVI 242

Query: 361 AIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDW 420
            +DVAA+ F    KYDLDFK+P      + +G  +  +Y++   +YP+VSIEDPFD++DW
Sbjct: 243 GMDVAASEFYRDGKYDLDFKSP-ADPSRYITGDQLGALYQDFVRNYPVVSIEDPFDQDDW 302

Query: 421 DHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAK 480
                F++    Q+VG DL ++N KRIERA++E  CN LLLKVNQIG+VTEAI+  KLA+
Sbjct: 303 AAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQ 362

Query: 481 DAQWAVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQA 540
           +  W V+ SHR GET+DTF+ADL VGL T QIK GAPCR ERLAKYNQL+RIEEELGD+A
Sbjct: 363 ENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEA 422

Query: 541 IYAGEDWR 547
            +AG ++R
Sbjct: 423 RFAGHNFR 429

BLAST of MELO3C020579 vs. ExPASy Swiss-Prot
Match: Q43130 (Enolase OS=Mesembryanthemum crystallinum OX=3544 GN=PGH1 PE=2 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.6e-140
Identity = 259/433 (59.82%), Postives = 328/433 (75.75%), Query Frame = 0

Query: 124 IKARQILDSRGIPTVEVDLYTNKGVF-HASVPSGDPAGMYEAVELRDGDKGTYLGNSVTK 183
           +KARQI DSRG PTVE D++ + G +  A+VPSG   G+YEA+ELRDG K  Y+G  V K
Sbjct: 7   VKARQIYDSRGNPTVEADIHLDDGTYARAAVPSGASTGVYEALELRDGGK-DYMGKGVYK 66

Query: 184 AVKNINEKISEALIGRDPTQQYEIDQAM-KDLDTTEK-----KGELGANAILAVSIAVCR 243
           AVKN+NE I  AL+G+DPTQQ  ID  M + LD T       K +LGANAILAVS+AVC+
Sbjct: 67  AVKNVNEIIGPALVGKDPTQQTAIDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCK 126

Query: 244 AGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEE 303
           AGA  K++PLY+HIA++AG  N++LPVPAF V++GG HAGN LA+QE MILP GAS F+E
Sbjct: 127 AGAQVKKIPLYQHIAEIAGNKNMVLPVPAFNVINGGSHAGNKLAMQEFMILPTGASSFKE 186

Query: 304 ALKMGSETFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERI 363
           A+KMGSE +H+LK+VI +K+G    N G++GGFAPNI   KE L+L++ AI + GY  ++
Sbjct: 187 AMKMGSEVYHNLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGYTGKV 246

Query: 364 KIAIDVAATNFCMGTK-YDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDR 423
            I +DVAA+ F    K YDL+FK  N       SG+ + D+YK   A+YPIVSIEDPFD+
Sbjct: 247 VIGMDVAASEFYKEDKSYDLNFKEENNDGSQRISGEALKDLYKSFVAEYPIVSIEDPFDQ 306

Query: 424 EDWDH-TKHFSSLG-ICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEV 483
           +DW+H  K  +  G   Q+VG DLL++N KR+++AIDE  CNALLLKVNQIG+VTE+IE 
Sbjct: 307 DDWEHYAKMTAECGEKVQIVGDDLLVTNPKRVKKAIDENPCNALLLKVNQIGSVTESIEA 366

Query: 484 VKLAKDAQWAVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEE 543
           VK++K A W V+ASHR GET+DTF+ADLSVGLST QIK GAPCR ERLAKYNQLLRIEEE
Sbjct: 367 VKMSKKAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLLRIEEE 426

Query: 544 LGDQAIYAGEDWR 547
           LGD+A+YAG ++R
Sbjct: 427 LGDKAVYAGANFR 438

BLAST of MELO3C020579 vs. NCBI nr
Match: XP_008457408.1 (PREDICTED: cytosolic enolase 3 [Cucumis melo])

HSP 1 Score: 965.3 bits (2494), Expect = 2.2e-277
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 67  MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 126
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 127 RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 186
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 187 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 246
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 247 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 306
           YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 307 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 366
           HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 367 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 426
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 427 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 486
           LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 487 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 546
           SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

Query: 547 AAC 550
           AAC
Sbjct: 481 AAC 483

BLAST of MELO3C020579 vs. NCBI nr
Match: XP_004145264.1 (cytosolic enolase 3 [Cucumis sativus] >KGN65781.1 hypothetical protein Csa_023204 [Cucumis sativus])

HSP 1 Score: 962.6 bits (2487), Expect = 1.4e-276
Identity = 482/483 (99.79%), Postives = 482/483 (99.79%), Query Frame = 0

Query: 67  MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 126
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 127 RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 186
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 187 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 246
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 247 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 306
           YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 307 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 366
           HLKAVITEKHGAH CNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAHGCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 367 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 426
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 427 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 486
           LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 487 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 546
           SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

Query: 547 AAC 550
           AAC
Sbjct: 481 AAC 483

BLAST of MELO3C020579 vs. NCBI nr
Match: TYJ97346.1 (cytosolic enolase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 954.9 bits (2467), Expect = 3.0e-274
Identity = 483/499 (96.79%), Postives = 483/499 (96.79%), Query Frame = 0

Query: 67  MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFI----------------S 126
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFI                S
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFIVISPFPSLCVVFLGNVS 60

Query: 127 NHMKKAIPSVITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDG 186
           NHMKKAIPSVITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDG
Sbjct: 61  NHMKKAIPSVITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDG 120

Query: 187 DKGTYLGNSVTKAVKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAV 246
           DKGTYLGNSVTKAVKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAV
Sbjct: 121 DKGTYLGNSVTKAVKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAV 180

Query: 247 SIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIG 306
           SIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIG
Sbjct: 181 SIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIG 240

Query: 307 ASKFEEALKMGSETFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRG 366
           ASKFEEALKMGSETFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRG
Sbjct: 241 ASKFEEALKMGSETFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRG 300

Query: 367 GYNERIKIAIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE 426
           GYNERIKIAIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE
Sbjct: 301 GYNERIKIAIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE 360

Query: 427 DPFDREDWDHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEA 486
           DPFDREDWDHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEA
Sbjct: 361 DPFDREDWDHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEA 420

Query: 487 IEVVKLAKDAQWAVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRI 546
           IEVVKLAKDAQWAVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRI
Sbjct: 421 IEVVKLAKDAQWAVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRI 480

Query: 547 EEELGDQAIYAGEDWRAAC 550
           EEELGDQAIYAGEDWRAAC
Sbjct: 481 EEELGDQAIYAGEDWRAAC 499

BLAST of MELO3C020579 vs. NCBI nr
Match: XP_038893436.1 (cytosolic enolase 3 [Benincasa hispida])

HSP 1 Score: 949.1 bits (2452), Expect = 1.7e-272
Identity = 474/483 (98.14%), Postives = 477/483 (98.76%), Query Frame = 0

Query: 67  MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 126
           MEKKQLKEMSVQEYLDK+MLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 127 RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 186
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 187 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 246
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 247 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 306
           YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGA KFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGACKFEEALKMGSETFH 240

Query: 307 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 366
           HLK VITEKHGAH CNFGEDGGFAPNISSFKEALDLVEEAI R GYNERIKIAIDVAATN
Sbjct: 241 HLKVVITEKHGAHGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATN 300

Query: 367 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 426
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 427 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 486
           LGICQVVGGDLLMSNKKR+ERAIDEFTCNALLLKVNQIGTVTEAIEVVK+AKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRMERAIDEFTCNALLLKVNQIGTVTEAIEVVKVAKDAQWAVVA 420

Query: 487 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 546
           SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQ IYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQGIYAGEDWR 480

Query: 547 AAC 550
           AAC
Sbjct: 481 AAC 483

BLAST of MELO3C020579 vs. NCBI nr
Match: XP_022949451.1 (cytosolic enolase 3 [Cucurbita moschata] >XP_023525808.1 cytosolic enolase 3 [Cucurbita pepo subsp. pepo] >KAG7036966.1 Cytosolic enolase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 921.8 bits (2381), Expect = 2.8e-264
Identity = 460/480 (95.83%), Postives = 469/480 (97.71%), Query Frame = 0

Query: 67  MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 126
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKA+PS+ITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60

Query: 127 RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 186
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAV+LRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120

Query: 187 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 246
           IN+KISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 247 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 306
           YKHIADLAGKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 307 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 366
           HLKAVI+EKHGAH CNFGEDGGFAPNISSF EALDLV+EAI R GYNERIKIAID+AATN
Sbjct: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300

Query: 367 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 426
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTKHFS 
Sbjct: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360

Query: 427 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 486
           LGICQVVGGDLLMSNKKRIERAIDE TCNALLLKVNQIGTVTEAIEVV LAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420

Query: 487 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 546
           SHRCGETDDTFLADLSVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

BLAST of MELO3C020579 vs. ExPASy TrEMBL
Match: A0A1S3C645 (Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103497104 PE=3 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.1e-277
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 67  MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 126
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 127 RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 186
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 187 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 246
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 247 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 306
           YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 307 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 366
           HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 367 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 426
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 427 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 486
           LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 487 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 546
           SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

Query: 547 AAC 550
           AAC
Sbjct: 481 AAC 483

BLAST of MELO3C020579 vs. ExPASy TrEMBL
Match: A0A0A0M0P5 (Phosphopyruvate hydratase OS=Cucumis sativus OX=3659 GN=Csa_1G528460 PE=3 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 7.0e-277
Identity = 482/483 (99.79%), Postives = 482/483 (99.79%), Query Frame = 0

Query: 67  MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 126
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 127 RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 186
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 187 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 246
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 247 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 306
           YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 307 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 366
           HLKAVITEKHGAH CNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAHGCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 367 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 426
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 427 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 486
           LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 487 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 546
           SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

Query: 547 AAC 550
           AAC
Sbjct: 481 AAC 483

BLAST of MELO3C020579 vs. ExPASy TrEMBL
Match: A0A5D3BEA1 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001560 PE=3 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.5e-274
Identity = 483/499 (96.79%), Postives = 483/499 (96.79%), Query Frame = 0

Query: 67  MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFI----------------S 126
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFI                S
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFIVISPFPSLCVVFLGNVS 60

Query: 127 NHMKKAIPSVITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDG 186
           NHMKKAIPSVITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDG
Sbjct: 61  NHMKKAIPSVITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDG 120

Query: 187 DKGTYLGNSVTKAVKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAV 246
           DKGTYLGNSVTKAVKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAV
Sbjct: 121 DKGTYLGNSVTKAVKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAV 180

Query: 247 SIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIG 306
           SIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIG
Sbjct: 181 SIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIG 240

Query: 307 ASKFEEALKMGSETFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRG 366
           ASKFEEALKMGSETFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRG
Sbjct: 241 ASKFEEALKMGSETFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRG 300

Query: 367 GYNERIKIAIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE 426
           GYNERIKIAIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE
Sbjct: 301 GYNERIKIAIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE 360

Query: 427 DPFDREDWDHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEA 486
           DPFDREDWDHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEA
Sbjct: 361 DPFDREDWDHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEA 420

Query: 487 IEVVKLAKDAQWAVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRI 546
           IEVVKLAKDAQWAVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRI
Sbjct: 421 IEVVKLAKDAQWAVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRI 480

Query: 547 EEELGDQAIYAGEDWRAAC 550
           EEELGDQAIYAGEDWRAAC
Sbjct: 481 EEELGDQAIYAGEDWRAAC 499

BLAST of MELO3C020579 vs. ExPASy TrEMBL
Match: A0A6J1GC24 (Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111452790 PE=3 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 1.4e-264
Identity = 460/480 (95.83%), Postives = 469/480 (97.71%), Query Frame = 0

Query: 67  MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 126
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKA+PS+ITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60

Query: 127 RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 186
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAV+LRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120

Query: 187 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 246
           IN+KISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 247 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 306
           YKHIADLAGKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 307 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 366
           HLKAVI+EKHGAH CNFGEDGGFAPNISSF EALDLV+EAI R GYNERIKIAID+AATN
Sbjct: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300

Query: 367 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 426
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTKHFS 
Sbjct: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360

Query: 427 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 486
           LGICQVVGGDLLMSNKKRIERAIDE TCNALLLKVNQIGTVTEAIEVV LAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420

Query: 487 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 546
           SHRCGETDDTFLADLSVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

BLAST of MELO3C020579 vs. ExPASy TrEMBL
Match: A0A6J1KFE0 (Phosphopyruvate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111493370 PE=3 SV=1)

HSP 1 Score: 915.6 bits (2365), Expect = 9.8e-263
Identity = 457/481 (95.01%), Postives = 470/481 (97.71%), Query Frame = 0

Query: 67  MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 126
           MEKKQLK+MSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKA+PS+ITKIKA
Sbjct: 1   MEKKQLKQMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60

Query: 127 RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 186
           RQILDSRGIPTVEVDL+TNKGVF+ASVPSGDPAGMYEAV+LRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLHTNKGVFNASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120

Query: 187 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 246
           IN+KISEALIGRDPTQQYEIDQAMK+LDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INDKISEALIGRDPTQQYEIDQAMKNLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 247 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 306
           YKHIADLAGKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 307 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 366
           HLKAVI+EKHGAH CNFGEDGGFAPNISSF EALDLV+EAI R GYNERIKIAID+AATN
Sbjct: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300

Query: 367 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 426
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTKHFS 
Sbjct: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360

Query: 427 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 486
           LGICQVVGGDLLMSNKKRIERAIDE TCNALLLKVNQIGTVTEAIEVV LAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420

Query: 487 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 546
           SHRCGETDDTFLADLSVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

Query: 547 A 548
           A
Sbjct: 481 A 481

BLAST of MELO3C020579 vs. TAIR 10
Match: AT2G29560.1 (cytosolic enolase )

HSP 1 Score: 783.1 bits (2021), Expect = 1.5e-226
Identity = 383/472 (81.14%), Postives = 429/472 (90.89%), Query Frame = 0

Query: 75  MSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKARQILDSRG 134
           MSVQEYLDK+MLSRKIE+AVNAAVRAKT DPVLFI+NH+KKA+ SVITK+KARQILDSRG
Sbjct: 1   MSVQEYLDKHMLSRKIEDAVNAAVRAKTSDPVLFIANHLKKAVSSVITKVKARQILDSRG 60

Query: 135 IPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKNINEKISEA 194
           IPTVEVDL+TNKGVF ASVPSGD +G YEA+ELRDGDKG YLGNSV KAVKNINEKISEA
Sbjct: 61  IPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSVAKAVKNINEKISEA 120

Query: 195 LIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPLYKHIADLA 254
           LIG DP  Q +IDQAM DLD TEKK ELGANAILAVSIA C+AGAAEKEVPL KH++DL+
Sbjct: 121 LIGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAAEKEVPLCKHLSDLS 180

Query: 255 GKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFHHLKAVITE 314
           G+ N++LPVPAFTVLSGGKHA N  AIQEIMILPIGAS+FEEAL+ GSET+HHLKAVI+E
Sbjct: 181 GRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQWGSETYHHLKAVISE 240

Query: 315 KHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATNFCMGTKYD 374
           K+G   CN GEDGG AP+ISS KE L+LV+EAINR GYN++IKIAID+AATNFC+GTKYD
Sbjct: 241 KNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAIDIAATNFCLGTKYD 300

Query: 375 LDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSSLGICQVVG 434
           LD K+PNKS QNFKS +DMIDMYKE+C DYPIVSIEDPFD+EDW+HTK+FSSLGICQVVG
Sbjct: 301 LDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWEHTKYFSSLGICQVVG 360

Query: 435 GDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVASHRCGETD 494
            DLLMSN KR+ERAI E +CNALLLKVNQIGTVTEAIEVVK+A+DAQW VV SHRCGET+
Sbjct: 361 DDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARDAQWGVVTSHRCGETE 420

Query: 495 DTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 547
           D+F++DLSVGL+T  IKAGAPCRGER  KYNQLLRIEEELGDQA+YAGEDW+
Sbjct: 421 DSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAVYAGEDWK 472

BLAST of MELO3C020579 vs. TAIR 10
Match: AT2G36530.1 (Enolase )

HSP 1 Score: 495.0 bits (1273), Expect = 8.0e-140
Identity = 258/438 (58.90%), Postives = 326/438 (74.43%), Query Frame = 0

Query: 119 SVITKIKARQILDSRGIPTVEVDLYTNKGV-FHASVPSGDPAGMYEAVELRDGDKGTYLG 178
           + IT +KARQI DSRG PTVEVD++T+ G+   A+VPSG   G+YEA+ELRDG    YLG
Sbjct: 2   ATITVVKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGG-SDYLG 61

Query: 179 NSVTKAVKNINEKISEALIGRDPTQQYEIDQAM-KDLDTTEK-----KGELGANAILAVS 238
             V+KAV N+N  I  ALIG+DPTQQ  ID  M  +LD T+      K +LGANAILAVS
Sbjct: 62  KGVSKAVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVS 121

Query: 239 IAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGA 298
           +AVC+AGA    +PLYKHIA+LAG   ++LPVPAF V++GG HAGN LA+QE MILP+GA
Sbjct: 122 LAVCKAGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 181

Query: 299 SKFEEALKMGSETFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGG 358
           + F+EA+KMG E +HHLK+VI +K+G    N G++GGFAPNI   KE L+L++ AI + G
Sbjct: 182 ASFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAG 241

Query: 359 YNERIKIAIDVAATNFCMGTK-YDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE 418
           Y  ++ I +DVAA+ F    K YDL+FK  N +     SG  + D+YK   A+YPIVSIE
Sbjct: 242 YTGKVVIGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIE 301

Query: 419 DPFDREDWDH-TKHFSSLGI-CQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVT 478
           DPFD++DW+H  K  +  G   Q+VG DLL++N KR+ +AI E +CNALLLKVNQIG+VT
Sbjct: 302 DPFDQDDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSVT 361

Query: 479 EAIEVVKLAKDAQWAVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLL 538
           E+IE VK++K A W V+ SHR GET+DTF+ADL+VGLST QIK GAPCR ERLAKYNQLL
Sbjct: 362 ESIEAVKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLL 421

Query: 539 RIEEELGDQAIYAGEDWR 547
           RIEEELG +AIYAG ++R
Sbjct: 422 RIEEELGSEAIYAGVNFR 438

BLAST of MELO3C020579 vs. TAIR 10
Match: AT1G74030.1 (enolase 1 )

HSP 1 Score: 468.8 bits (1205), Expect = 6.1e-132
Identity = 237/425 (55.76%), Postives = 307/425 (72.24%), Query Frame = 0

Query: 124 IKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKA 183
           +KARQI+DSRG PTVEVDL T+  ++ ++VPSG   G+YEA+ELRDGDK  Y G  V +A
Sbjct: 54  VKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQA 113

Query: 184 VKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKE 243
           +KNINE ++  LIG D   Q ++D  M +LD T  K +LGANAIL VS++VCRAGA  K 
Sbjct: 114 IKNINELVAPKLIGVDVRNQADVDALMLELDGTPNKSKLGANAILGVSLSVCRAGAGAKG 173

Query: 244 VPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSE 303
           VPLYKHI + +G   L++PVPAF V++GG HAGN+LA+QE MILP+GA+ F EA +MGSE
Sbjct: 174 VPLYKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSE 233

Query: 304 TFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVA 363
            +H LK +I  K+G   CN G++GGFAPN+   +E L L+ +AI + GY  +IKI +DVA
Sbjct: 234 VYHTLKGIIKTKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVA 293

Query: 364 ATNFCM-GTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTK 423
           A+ F M   +YDL+FK       +  S + + D+Y+E   D+PIVSIEDPFD++DW    
Sbjct: 294 ASEFFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQDDWSSWA 353

Query: 424 HFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQW 483
              S    Q+VG DLL++N KRI  AI + +CNALLLKVNQIGTVTE+I+    +K A W
Sbjct: 354 SLQSSVDIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQAALDSKAAGW 413

Query: 484 AVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAG 543
            V+ SHR GET+D F+ADLSVGL++ QIK GAPCR ERL+KYNQLLRIEEELG+   YAG
Sbjct: 414 GVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEEELGN-VRYAG 473

Query: 544 EDWRA 548
           E +R+
Sbjct: 474 EAFRS 476