Homology
BLAST of MELO3C020541.jh1 vs. NCBI nr
Match:
KAA0031835.1 (acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum [Cucumis melo var. makuwa])
HSP 1 Score: 795 bits (2054), Expect = 4.50e-291
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 0
Query: 1 MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR
Sbjct: 1 MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
Query: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL
Sbjct: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
Query: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
Query: 361 DQGNIVFYQTDPYLYHY 377
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 377
BLAST of MELO3C020541.jh1 vs. NCBI nr
Match:
XP_008457364.1 (PREDICTED: acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum [Cucumis melo])
HSP 1 Score: 784 bits (2024), Expect = 1.57e-286
Identity = 374/377 (99.20%), Postives = 374/377 (99.20%), Query Frame = 0
Query: 1 MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPANGNGN N ATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR
Sbjct: 1 MAIPLPANGNGNSN--ATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
Query: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL
Sbjct: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
Query: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
Query: 361 DQGNIVFYQTDPYLYHY 377
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 375
BLAST of MELO3C020541.jh1 vs. NCBI nr
Match:
XP_004145254.1 (acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum [Cucumis sativus] >KGN65813.1 hypothetical protein Csa_023305 [Cucumis sativus])
HSP 1 Score: 750 bits (1937), Expect = 2.47e-273
Identity = 356/377 (94.43%), Postives = 363/377 (96.29%), Query Frame = 0
Query: 1 MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPL AN N ATAAAP PFDPSAPPPFRI+EIRAAIPPHCWVK+PWRSL+YVLR
Sbjct: 1 MAIPLLANAN------ATAAAPAPFDPSAPPPFRISEIRAAIPPHCWVKSPWRSLLYVLR 60
Query: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
DLA+VSALVAAA+FFDSWL WPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL
Sbjct: 61 DLAIVSALVAAALFFDSWLVWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLE+RTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAP ER+DIVISTSCWTLMA LLVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPGERRDIVISTSCWTLMAALLVYLSFVFGPFQIFK 240
Query: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLV NLVNSLKQDHYVS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPTHLVKNLVNSLKQDHYVS 360
Query: 361 DQGNIVFYQTDPYLYHY 377
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 371
BLAST of MELO3C020541.jh1 vs. NCBI nr
Match:
XP_038893495.1 (LOW QUALITY PROTEIN: omega-3 fatty acid desaturase, endoplasmic reticulum-like [Benincasa hispida])
HSP 1 Score: 733 bits (1893), Expect = 1.46e-266
Identity = 348/377 (92.31%), Postives = 359/377 (95.23%), Query Frame = 0
Query: 1 MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPA+G GNGN ATAA P PFDPSAPPPFR+ +IRAAIPPHCWVKNPWRSLVYVLR
Sbjct: 1 MAIPLPASGTGNGN--ATAATPAPFDPSAPPPFRMXDIRAAIPPHCWVKNPWRSLVYVLR 60
Query: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
DL +VSALV AVFFDSWLFWPIY +AQGTMFWA FVLGHDCGHGSFSNS ALNSFMGLL
Sbjct: 61 DLVIVSALVGRAVFFDSWLFWPIYLVAQGTMFWAXFVLGHDCGHGSFSNSYALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
LHSFILVP RISHRTHHQNHGNVEKDESWVPLTKKTYKQLE+RTRILRFTLPFPILA
Sbjct: 121 LHSFILVPDQELRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAP+ERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
Query: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
+YGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEW+YLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 IYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLVNNLVNSL+QDHYV+
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVNNLVNSLRQDHYVN 360
Query: 361 DQGNIVFYQTDPYLYHY 377
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 375
BLAST of MELO3C020541.jh1 vs. NCBI nr
Match:
XP_022948579.1 (omega-3 fatty acid desaturase, endoplasmic reticulum-like [Cucurbita moschata])
HSP 1 Score: 725 bits (1871), Expect = 3.69e-263
Identity = 338/378 (89.42%), Postives = 358/378 (94.71%), Query Frame = 0
Query: 1 MAIPLPANGNGNGNGNATA-AAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVL 60
MAIPLPA+ GN +A AAP PFDPSAPPPFRI+EIRAAIPPHCWVKNPWRSL+YVL
Sbjct: 1 MAIPLPASRPGNDTVHANGTAAPHPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLLYVL 60
Query: 61 RDLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
RD ++SALVAAA +FDSWL WPIYW+AQGTMFWAIFVLGHDCGHGSFSNS+ALNSFMGL
Sbjct: 61 RDFVIISALVAAAAYFDSWLVWPIYWIAQGTMFWAIFVLGHDCGHGSFSNSTALNSFMGL 120
Query: 121 LLHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPIL 180
+LHS ILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRF+LPFPI
Sbjct: 121 ILHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFSLPFPIF 180
Query: 181 AYPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIF 240
AYPFYLMWRSPGKEGSHFNPYSD+FAP+ERKDIVISTSCWT+MAVLLVYLSFVFGP QIF
Sbjct: 181 AYPFYLMWRSPGKEGSHFNPYSDIFAPNERKDIVISTSCWTIMAVLLVYLSFVFGPTQIF 240
Query: 241 KLYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300
KLYGVPYW+FV+WLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGL NNIHHD
Sbjct: 241 KLYGVPYWVFVIWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLINNIHHD 300
Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYV 360
IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP+HLV NLVNS +QDHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPLHLVKNLVNSFRQDHYV 360
Query: 361 SDQGNIVFYQTDPYLYHY 377
SD+GN+VFYQTDPYLY Y
Sbjct: 361 SDEGNVVFYQTDPYLYSY 378
BLAST of MELO3C020541.jh1 vs. ExPASy Swiss-Prot
Match:
P48622 (Temperature-sensitive sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD8 PE=1 SV=1)
HSP 1 Score: 581.6 bits (1498), Expect = 6.2e-165
Identity = 257/350 (73.43%), Postives = 300/350 (85.71%), Query Frame = 0
Query: 25 FDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLRDLAVVSALVAAAVFFDSWLFWPIY 84
FDP APPPF +A+IRAAIP HCWVKNPW S+ YV+RD+A+V L A A +F++WL WP+Y
Sbjct: 81 FDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLY 140
Query: 85 WLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSFILVPYHGWRISHRTHHQNHG 144
W AQGTMFWA+FVLGHDCGHGSFSN LNS G LLHS ILVPYHGWRISHRTHHQNHG
Sbjct: 141 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 200
Query: 145 NVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSDLF 204
+VE DESW PL + YK LE+ T++ RFTLPFP+LAYPFYL RSPGK+GSH++P SDLF
Sbjct: 201 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 260
Query: 205 APSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFKLYGVPYWIFVMWLDVVTYLHHHGY 264
P E+KD++ ST+CWT MA LLV L+FV GP Q+ KLYG+PYWIFVMWLD VTYLHHHG+
Sbjct: 261 LPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGH 320
Query: 265 EQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 324
E KLPWYRG+EW+YLRGGLTT+DRDYG NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AA
Sbjct: 321 EDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAA 380
Query: 325 KGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVSDQGNIVFYQTDPYL 375
K VLGKYYREPK SGP+P+HL+ +L+ S+KQDH+VSD G++V+Y+ DP L
Sbjct: 381 KPVLGKYYREPKNSGPLPLHLLGSLIKSMKQDHFVSDTGDVVYYEADPKL 430
BLAST of MELO3C020541.jh1 vs. ExPASy Swiss-Prot
Match:
P48624 (Acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum OS=Brassica napus OX=3708 GN=FAD3 PE=1 SV=1)
HSP 1 Score: 581.6 bits (1498), Expect = 6.2e-165
Identity = 267/366 (72.95%), Postives = 310/366 (84.70%), Query Frame = 0
Query: 12 NGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLRDLAVVSALVAA 71
N NG++ A FDPSA PPF+I +IRAAIP HCWVK+P RS+ YV RD+ V+AL A
Sbjct: 10 NVNGDSGARKEEGFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVTRDIFAVAALAMA 69
Query: 72 AVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSFILVPYHG 131
AV+FDSW WP+YW+AQGT+FWAIFVLGHDCGHGSFS+ LNS +G +LHSFILVPYHG
Sbjct: 70 AVYFDSWFLWPLYWVAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHG 129
Query: 132 WRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPG 191
WRISHRTHHQNHG+VE DESWVPL +K YK L TR+LR+T+P P+LAYP YL +RSPG
Sbjct: 130 WRISHRTHHQNHGHVENDESWVPLPEKLYKNLPHSTRMLRYTVPLPMLAYPIYLWYRSPG 189
Query: 192 KEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFKLYGVPYWIFVM 251
KEGSHFNPYS LFAPSERK I ST+CW++M LVYLSF+ P + K+YGVPY IFVM
Sbjct: 190 KEGSHFNPYSSLFAPSERKLIATSTTCWSIMLATLVYLSFLVDPVTVLKVYGVPYIIFVM 249
Query: 252 WLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQ 311
WLD VTYLHHHG+++KLPWYRG+EW+YLRGGLTT+DRDYG+FNNIHHDIGTHVIHHLFPQ
Sbjct: 250 WLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHDIGTHVIHHLFPQ 309
Query: 312 IPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVSDQGNIVFYQTD 371
IPHYHLV+AT+AAK VLG+YYREPK SG IPIHLV +LV S+K+DHYVSD G+IVFY+TD
Sbjct: 310 IPHYHLVDATRAAKHVLGRYYREPKTSGAIPIHLVESLVASIKKDHYVSDTGDIVFYETD 369
Query: 372 PYLYHY 378
P LY Y
Sbjct: 370 PDLYVY 375
BLAST of MELO3C020541.jh1 vs. ExPASy Swiss-Prot
Match:
P48625 (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Glycine max OX=3847 GN=FAD3 PE=2 SV=1)
HSP 1 Score: 580.1 bits (1494), Expect = 1.8e-164
Identity = 263/363 (72.45%), Postives = 303/363 (83.47%), Query Frame = 0
Query: 14 NGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLRDLAVVSALVAAAV 73
NG + FDPSAPPPF+IAEIRA+IP HCWVKNPWRSL YVLRD+ V++ALVAAA+
Sbjct: 14 NGYQQKGSSFDFDPSAPPPFKIAEIRASIPKHCWVKNPWRSLSYVLRDVLVIAALVAAAI 73
Query: 74 FFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSFILVPYHGWR 133
FD+WL W IY QGTMFWA+FVLGHDCGHGSFS+S LNS +G +LHS ILVPYHGWR
Sbjct: 74 HFDNWLLWLIYCPIQGTMFWALFVLGHDCGHGSFSDSPLLNSLVGHILHSSILVPYHGWR 133
Query: 134 ISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKE 193
ISHRTHHQNHG++EKDESWVPLT+K YK L+ TR++RFT+PFP+ YP YL RSPGKE
Sbjct: 134 ISHRTHHQNHGHIEKDESWVPLTEKIYKNLDSMTRLIRFTVPFPLFVYPIYLFSRSPGKE 193
Query: 194 GSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFKLYGVPYWIFVMWL 253
GSHFNPYS+LF PSERK I IST CW M LL+YLSF+ P + KLYG+PYWIFVMWL
Sbjct: 194 GSHFNPYSNLFPPSERKGIAISTLCWATMFSLLIYLSFITSPLLVLKLYGIPYWIFVMWL 253
Query: 254 DVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIP 313
D VTYLHHHG+ QKLPWYRG+EW+YLRGGLTTVDRDYG NIHHDIGTHVIHHLFPQIP
Sbjct: 254 DFVTYLHHHGHHQKLPWYRGKEWSYLRGGLTTVDRDYGWIYNIHHDIGTHVIHHLFPQIP 313
Query: 314 HYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVSDQGNIVFYQTDPY 373
HYHLVEAT+AAK VLG YYREP++S P+P HL+ L+ S++QDH+VSD G++V+YQTD
Sbjct: 314 HYHLVEATQAAKPVLGDYYREPERSAPLPFHLIKYLIQSMRQDHFVSDTGDVVYYQTDSL 373
Query: 374 LYH 377
L H
Sbjct: 374 LLH 376
BLAST of MELO3C020541.jh1 vs. ExPASy Swiss-Prot
Match:
P48623 (Acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum OS=Arabidopsis thaliana OX=3702 GN=FAD3 PE=1 SV=1)
HSP 1 Score: 579.7 bits (1493), Expect = 2.4e-164
Identity = 269/378 (71.16%), Postives = 314/378 (83.07%), Query Frame = 0
Query: 1 MAIPLPANGNGNGN-GNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVL 60
M + + N NG+ G FDPSA PPF+I +IRAAIP HCWVK+P RS+ YV+
Sbjct: 1 MVVAMDQRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVV 60
Query: 61 RDLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
RD+ V+AL AAV+ DSW WP+YW AQGT+FWAIFVLGHDCGHGSFS+ LNS +G
Sbjct: 61 RDIIAVAALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGH 120
Query: 121 LLHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPIL 180
+LHSFILVPYHGWRISHRTHHQNHG+VE DESWVPL ++ YK+L TR+LR+T+P P+L
Sbjct: 121 ILHSFILVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPML 180
Query: 181 AYPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIF 240
AYP YL +RSPGKEGSHFNPYS LFAPSERK I ST+CW++M V L+ LSFVFGP +
Sbjct: 181 AYPLYLCYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVL 240
Query: 241 KLYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300
K+YGVPY IFVMWLD VTYLHHHG+++KLPWYRG+EW+YLRGGLTT+DRDYG+FNNIHHD
Sbjct: 241 KVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHD 300
Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYV 360
IGTHVIHHLFPQIPHYHLV+ATKAAK VLG+YYREPK SG IPIHLV +LV S+K+DHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVDATKAAKHVLGRYYREPKTSGAIPIHLVESLVASIKKDHYV 360
Query: 361 SDQGNIVFYQTDPYLYHY 378
SD G+IVFY+TDP LY Y
Sbjct: 361 SDTGDIVFYETDPDLYVY 378
BLAST of MELO3C020541.jh1 vs. ExPASy Swiss-Prot
Match:
P48626 (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Nicotiana tabacum OX=4097 GN=FAD3 PE=2 SV=1)
HSP 1 Score: 579.7 bits (1493), Expect = 2.4e-164
Identity = 263/351 (74.93%), Postives = 298/351 (84.90%), Query Frame = 0
Query: 25 FDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLRDLAVVSALVAAAVFFDSWLFWPIY 84
FDPSAPPPFR+AEIR IP HCWVK+P RSL YV+RD+ V+ L+ A+ DSWLF+P+Y
Sbjct: 22 FDPSAPPPFRLAEIRNVIPKHCWVKDPLRSLSYVVRDVIFVATLIGIAIHLDSWLFYPLY 81
Query: 85 WLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSFILVPYHGWRISHRTHHQNHG 144
W QGTMFWAIFVLGHDCGHGSFS+S LN+ +G +LHS ILVPYHGWRISH+THHQNHG
Sbjct: 82 WAIQGTMFWAIFVLGHDCGHGSFSDSQLLNNVVGHILHSAILVPYHGWRISHKTHHQNHG 141
Query: 145 NVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSDLF 204
NVE DESWVP+ +K Y ++ T+ LR+ +PFP+LAYP YLM RSPGK GSHFNPYSDLF
Sbjct: 142 NVETDESWVPMPEKLYNKVGYSTKFLRYKIPFPLLAYPMYLMKRSPGKSGSHFNPYSDLF 201
Query: 205 APSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFKLYGVPYWIFVMWLDVVTYLHHHGY 264
P ERK +V ST CWT+MA LL+YL FG Q+FK+YG PY IFVMWLD VTYLHHHGY
Sbjct: 202 QPHERKYVVTSTLCWTVMAALLLYLCTAFGSLQMFKIYGAPYLIFVMWLDFVTYLHHHGY 261
Query: 265 EQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 324
E+KLPWYRG+EW+YLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHL EATKAA
Sbjct: 262 EKKLPWYRGKEWSYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLREATKAA 321
Query: 325 KGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVSDQGNIVFYQTDPYLY 376
K VLGKYYREPKKSGPIP HLV +L S+KQDHYVSD G IVFYQTDP+++
Sbjct: 322 KPVLGKYYREPKKSGPIPFHLVKDLTRSMKQDHYVSDSGEIVFYQTDPHIF 372
BLAST of MELO3C020541.jh1 vs. ExPASy TrEMBL
Match:
A0A5A7SRR2 (Acyl-lipid omega-3 desaturase (Cytochrome b5), endoplasmic reticulum OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00850 PE=3 SV=1)
HSP 1 Score: 795 bits (2054), Expect = 2.18e-291
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 0
Query: 1 MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR
Sbjct: 1 MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
Query: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL
Sbjct: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
Query: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
Query: 361 DQGNIVFYQTDPYLYHY 377
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 377
BLAST of MELO3C020541.jh1 vs. ExPASy TrEMBL
Match:
A0A1S3C608 (acyl-lipid omega-3 desaturase (Cytochrome b5), endoplasmic reticulum OS=Cucumis melo OX=3656 GN=LOC103497071 PE=3 SV=1)
HSP 1 Score: 784 bits (2024), Expect = 7.58e-287
Identity = 374/377 (99.20%), Postives = 374/377 (99.20%), Query Frame = 0
Query: 1 MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPANGNGN N ATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR
Sbjct: 1 MAIPLPANGNGNSN--ATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
Query: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL
Sbjct: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
Query: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
Query: 361 DQGNIVFYQTDPYLYHY 377
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 375
BLAST of MELO3C020541.jh1 vs. ExPASy TrEMBL
Match:
A0A0A0LVJ0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G532210 PE=3 SV=1)
HSP 1 Score: 750 bits (1937), Expect = 1.20e-273
Identity = 356/377 (94.43%), Postives = 363/377 (96.29%), Query Frame = 0
Query: 1 MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPL AN N ATAAAP PFDPSAPPPFRI+EIRAAIPPHCWVK+PWRSL+YVLR
Sbjct: 1 MAIPLLANAN------ATAAAPAPFDPSAPPPFRISEIRAAIPPHCWVKSPWRSLLYVLR 60
Query: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
DLA+VSALVAAA+FFDSWL WPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL
Sbjct: 61 DLAIVSALVAAALFFDSWLVWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLE+RTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
YPFYLMWRSPGKEGSHFNPYSDLFAP ER+DIVISTSCWTLMA LLVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPGERRDIVISTSCWTLMAALLVYLSFVFGPFQIFK 240
Query: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLV NLVNSLKQDHYVS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPTHLVKNLVNSLKQDHYVS 360
Query: 361 DQGNIVFYQTDPYLYHY 377
DQGNIVFYQTDPYLYHY
Sbjct: 361 DQGNIVFYQTDPYLYHY 371
BLAST of MELO3C020541.jh1 vs. ExPASy TrEMBL
Match:
A0A6J1GAA9 (omega-3 fatty acid desaturase, endoplasmic reticulum-like OS=Cucurbita moschata OX=3662 GN=LOC111452212 PE=3 SV=1)
HSP 1 Score: 725 bits (1871), Expect = 1.79e-263
Identity = 338/378 (89.42%), Postives = 358/378 (94.71%), Query Frame = 0
Query: 1 MAIPLPANGNGNGNGNATA-AAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVL 60
MAIPLPA+ GN +A AAP PFDPSAPPPFRI+EIRAAIPPHCWVKNPWRSL+YVL
Sbjct: 1 MAIPLPASRPGNDTVHANGTAAPHPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLLYVL 60
Query: 61 RDLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
RD ++SALVAAA +FDSWL WPIYW+AQGTMFWAIFVLGHDCGHGSFSNS+ALNSFMGL
Sbjct: 61 RDFVIISALVAAAAYFDSWLVWPIYWIAQGTMFWAIFVLGHDCGHGSFSNSTALNSFMGL 120
Query: 121 LLHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPIL 180
+LHS ILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRF+LPFPI
Sbjct: 121 ILHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFSLPFPIF 180
Query: 181 AYPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIF 240
AYPFYLMWRSPGKEGSHFNPYSD+FAP+ERKDIVISTSCWT+MAVLLVYLSFVFGP QIF
Sbjct: 181 AYPFYLMWRSPGKEGSHFNPYSDIFAPNERKDIVISTSCWTIMAVLLVYLSFVFGPTQIF 240
Query: 241 KLYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300
KLYGVPYW+FV+WLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGL NNIHHD
Sbjct: 241 KLYGVPYWVFVIWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLINNIHHD 300
Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYV 360
IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP+HLV NLVNS +QDHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPLHLVKNLVNSFRQDHYV 360
Query: 361 SDQGNIVFYQTDPYLYHY 377
SD+GN+VFYQTDPYLY Y
Sbjct: 361 SDEGNVVFYQTDPYLYSY 378
BLAST of MELO3C020541.jh1 vs. ExPASy TrEMBL
Match:
A0A6J1I635 (omega-3 fatty acid desaturase, endoplasmic reticulum-like OS=Cucurbita maxima OX=3661 GN=LOC111469542 PE=3 SV=1)
HSP 1 Score: 722 bits (1864), Expect = 1.79e-262
Identity = 337/376 (89.63%), Postives = 357/376 (94.95%), Query Frame = 0
Query: 1 MAIPLPANGNGNGNGNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLR 60
MAIPLPA+ NG A A PFDPSAPPPFRIAEIRAAIPPHCWVK+PWRSL+YVLR
Sbjct: 1 MAIPLPASATATANGTAALA---PFDPSAPPPFRIAEIRAAIPPHCWVKSPWRSLLYVLR 60
Query: 61 DLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLL 120
DLAVVSAL+AAAVFFDSWL WPIYW+AQGTMFWAIFVLGHDCGHGSFSNS+ALN+ +GL+
Sbjct: 61 DLAVVSALIAAAVFFDSWLLWPIYWVAQGTMFWAIFVLGHDCGHGSFSNSTALNTLVGLV 120
Query: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA
Sbjct: 121 LHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILA 180
Query: 181 YPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFK 240
YPFYLMWRSPGK+GSHFNP SDLFAP+ERKDIVISTSCW LMA +LVYLSFVFGPFQIFK
Sbjct: 181 YPFYLMWRSPGKKGSHFNPNSDLFAPNERKDIVISTSCWALMAAVLVYLSFVFGPFQIFK 240
Query: 241 LYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDI 300
+YGVPYWIFVMWLD+VTYLHHHGYEQKLPWYRGEEW+YLRGGLTTVDRDYGLFNNIHHDI
Sbjct: 241 IYGVPYWIFVMWLDLVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLFNNIHHDI 300
Query: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVS 360
GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHL+ NLVNS ++DH+VS
Sbjct: 301 GTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLMENLVNSFREDHFVS 360
Query: 361 DQGNIVFYQTDPYLYH 376
D GNIVFYQTDPYL+H
Sbjct: 361 DNGNIVFYQTDPYLHH 373
BLAST of MELO3C020541.jh1 vs. TAIR 10
Match:
AT5G05580.1 (fatty acid desaturase 8 )
HSP 1 Score: 581.6 bits (1498), Expect = 4.4e-166
Identity = 257/350 (73.43%), Postives = 300/350 (85.71%), Query Frame = 0
Query: 25 FDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLRDLAVVSALVAAAVFFDSWLFWPIY 84
FDP APPPF +A+IRAAIP HCWVKNPW S+ YV+RD+A+V L A A +F++WL WP+Y
Sbjct: 81 FDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLY 140
Query: 85 WLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSFILVPYHGWRISHRTHHQNHG 144
W AQGTMFWA+FVLGHDCGHGSFSN LNS G LLHS ILVPYHGWRISHRTHHQNHG
Sbjct: 141 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 200
Query: 145 NVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSDLF 204
+VE DESW PL + YK LE+ T++ RFTLPFP+LAYPFYL RSPGK+GSH++P SDLF
Sbjct: 201 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 260
Query: 205 APSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFKLYGVPYWIFVMWLDVVTYLHHHGY 264
P E+KD++ ST+CWT MA LLV L+FV GP Q+ KLYG+PYWIFVMWLD VTYLHHHG+
Sbjct: 261 LPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGH 320
Query: 265 EQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 324
E KLPWYRG+EW+YLRGGLTT+DRDYG NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AA
Sbjct: 321 EDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAA 380
Query: 325 KGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVSDQGNIVFYQTDPYL 375
K VLGKYYREPK SGP+P+HL+ +L+ S+KQDH+VSD G++V+Y+ DP L
Sbjct: 381 KPVLGKYYREPKNSGPLPLHLLGSLIKSMKQDHFVSDTGDVVYYEADPKL 430
BLAST of MELO3C020541.jh1 vs. TAIR 10
Match:
AT2G29980.1 (fatty acid desaturase 3 )
HSP 1 Score: 579.7 bits (1493), Expect = 1.7e-165
Identity = 269/378 (71.16%), Postives = 314/378 (83.07%), Query Frame = 0
Query: 1 MAIPLPANGNGNGN-GNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVL 60
M + + N NG+ G FDPSA PPF+I +IRAAIP HCWVK+P RS+ YV+
Sbjct: 1 MVVAMDQRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVV 60
Query: 61 RDLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
RD+ V+AL AAV+ DSW WP+YW AQGT+FWAIFVLGHDCGHGSFS+ LNS +G
Sbjct: 61 RDIIAVAALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGH 120
Query: 121 LLHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPIL 180
+LHSFILVPYHGWRISHRTHHQNHG+VE DESWVPL ++ YK+L TR+LR+T+P P+L
Sbjct: 121 ILHSFILVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPML 180
Query: 181 AYPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIF 240
AYP YL +RSPGKEGSHFNPYS LFAPSERK I ST+CW++M V L+ LSFVFGP +
Sbjct: 181 AYPLYLCYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVL 240
Query: 241 KLYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300
K+YGVPY IFVMWLD VTYLHHHG+++KLPWYRG+EW+YLRGGLTT+DRDYG+FNNIHHD
Sbjct: 241 KVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHD 300
Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYV 360
IGTHVIHHLFPQIPHYHLV+ATKAAK VLG+YYREPK SG IPIHLV +LV S+K+DHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVDATKAAKHVLGRYYREPKTSGAIPIHLVESLVASIKKDHYV 360
Query: 361 SDQGNIVFYQTDPYLYHY 378
SD G+IVFY+TDP LY Y
Sbjct: 361 SDTGDIVFYETDPDLYVY 378
BLAST of MELO3C020541.jh1 vs. TAIR 10
Match:
AT3G11170.1 (fatty acid desaturase 7 )
HSP 1 Score: 575.9 bits (1483), Expect = 2.4e-164
Identity = 256/351 (72.93%), Postives = 299/351 (85.19%), Query Frame = 0
Query: 25 FDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLRDLAVVSALVAAAVFFDSWLFWPIY 84
FDP APPPF +A+IRAAIP HCWVKNPW+SL YV+RD+A+V AL A A + ++W+ WP+Y
Sbjct: 88 FDPGAPPPFNLADIRAAIPKHCWVKNPWKSLSYVVRDVAIVFALAAGAAYLNNWIVWPLY 147
Query: 85 WLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSFILVPYHGWRISHRTHHQNHG 144
WLAQGTMFWA+FVLGHDCGHGSFSN LNS +G LLHS ILVPYHGWRISHRTHHQNHG
Sbjct: 148 WLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHG 207
Query: 145 NVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSDLF 204
+VE DESW P+++K Y L++ TR RFTLP +LAYPFYL RSPGK+GSH++P SDLF
Sbjct: 208 HVENDESWHPMSEKIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSHYHPDSDLF 267
Query: 205 APSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFKLYGVPYWIFVMWLDVVTYLHHHGY 264
P ERKD++ ST+CWT MA LLV L+F GP Q+ KLYG+PYWI VMWLD VTYLHHHG+
Sbjct: 268 LPKERKDVLTSTACWTAMAALLVCLNFTIGPIQMLKLYGIPYWINVMWLDFVTYLHHHGH 327
Query: 265 EQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 324
E KLPWYRG+EW+YLRGGLTT+DRDYGL NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AA
Sbjct: 328 EDKLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDIGTHVIHHLFPQIPHYHLVEATEAA 387
Query: 325 KGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYVSDQGNIVFYQTDPYLY 376
K VLGKYYREP KSGP+P+HL+ L S+K+DHYVSD+G +V+Y+ DP LY
Sbjct: 388 KPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVSDEGEVVYYKADPNLY 438
BLAST of MELO3C020541.jh1 vs. TAIR 10
Match:
AT5G05580.2 (fatty acid desaturase 8 )
HSP 1 Score: 500.7 bits (1288), Expect = 1.0e-141
Identity = 222/296 (75.00%), Postives = 253/296 (85.47%), Query Frame = 0
Query: 25 FDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVLRDLAVVSALVAAAVFFDSWLFWPIY 84
FDP APPPF +A+IRAAIP HCWVKNPW S+ YV+RD+A+V L A A +F++WL WP+Y
Sbjct: 81 FDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLY 140
Query: 85 WLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSFILVPYHGWRISHRTHHQNHG 144
W AQGTMFWA+FVLGHDCGHGSFSN LNS G LLHS ILVPYHGWRISHRTHHQNHG
Sbjct: 141 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 200
Query: 145 NVEKDESWVPLTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSDLF 204
+VE DESW PL + YK LE+ T++ RFTLPFP+LAYPFYL RSPGK+GSH++P SDLF
Sbjct: 201 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 260
Query: 205 APSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIFKLYGVPYWIFVMWLDVVTYLHHHGY 264
P E+KD++ ST+CWT MA LLV L+FV GP Q+ KLYG+PYWIFVMWLD VTYLHHHG+
Sbjct: 261 LPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGH 320
Query: 265 EQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEA 321
E KLPWYRG+EW+YLRGGLTT+DRDYG NNIHHDIGTHVIHHLFPQIPHYHLVEA
Sbjct: 321 EDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEA 376
BLAST of MELO3C020541.jh1 vs. TAIR 10
Match:
AT2G29980.2 (fatty acid desaturase 3 )
HSP 1 Score: 397.5 bits (1020), Expect = 1.2e-110
Identity = 184/275 (66.91%), Postives = 219/275 (79.64%), Query Frame = 0
Query: 1 MAIPLPANGNGNGN-GNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVL 60
M + + N NG+ G FDPSA PPF+I +IRAAIP HCWVK+P RS+ YV+
Sbjct: 1 MVVAMDQRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVV 60
Query: 61 RDLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
RD+ V+AL AAV+ DSW WP+YW AQGT+FWAIFVLGHDCGHGSFS+ LNS +G
Sbjct: 61 RDIIAVAALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGH 120
Query: 121 LLHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPIL 180
+LHSFILVPYHGWRISHRTHHQNHG+VE DESWVPL ++ YK+L TR+LR+T+P P+L
Sbjct: 121 ILHSFILVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPML 180
Query: 181 AYPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIF 240
AYP YL +RSPGKEGSHFNPYS LFAPSERK I ST+CW++M V L+ LSFVFGP +
Sbjct: 181 AYPLYLCYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVL 240
Query: 241 KLYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGE 275
K+YGVPY IFVMWLD VTYLHHHG+++KLPWYRG+
Sbjct: 241 KVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGK 275
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAA0031835.1 | 4.50e-291 | 100.00 | acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum [Cucumis me... | [more] |
XP_008457364.1 | 1.57e-286 | 99.20 | PREDICTED: acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum ... | [more] |
XP_004145254.1 | 2.47e-273 | 94.43 | acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum [Cucumis sa... | [more] |
XP_038893495.1 | 1.46e-266 | 92.31 | LOW QUALITY PROTEIN: omega-3 fatty acid desaturase, endoplasmic reticulum-like [... | [more] |
XP_022948579.1 | 3.69e-263 | 89.42 | omega-3 fatty acid desaturase, endoplasmic reticulum-like [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
P48622 | 6.2e-165 | 73.43 | Temperature-sensitive sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloropla... | [more] |
P48624 | 6.2e-165 | 72.95 | Acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum OS=Brassica... | [more] |
P48625 | 1.8e-164 | 72.45 | Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Glycine max OX=3847 GN=F... | [more] |
P48623 | 2.4e-164 | 71.16 | Acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum OS=Arabidop... | [more] |
P48626 | 2.4e-164 | 74.93 | Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Nicotiana tabacum OX=409... | [more] |
Match Name | E-value | Identity | Description | |
A0A5A7SRR2 | 2.18e-291 | 100.00 | Acyl-lipid omega-3 desaturase (Cytochrome b5), endoplasmic reticulum OS=Cucumis ... | [more] |
A0A1S3C608 | 7.58e-287 | 99.20 | acyl-lipid omega-3 desaturase (Cytochrome b5), endoplasmic reticulum OS=Cucumis ... | [more] |
A0A0A0LVJ0 | 1.20e-273 | 94.43 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G532210 PE=3 SV=1 | [more] |
A0A6J1GAA9 | 1.79e-263 | 89.42 | omega-3 fatty acid desaturase, endoplasmic reticulum-like OS=Cucurbita moschata ... | [more] |
A0A6J1I635 | 1.79e-262 | 89.63 | omega-3 fatty acid desaturase, endoplasmic reticulum-like OS=Cucurbita maxima OX... | [more] |