MELO3C020496.jh1 (gene) Melon (Harukei-3) v1.41

BLAST of MELO3C020496.jh1 vs. NCBI nr
Match: XP_008457307.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo])

HSP 1 Score: 999 bits (2583), Expect = 0.0
Identity = 526/531 (99.06%), Postives = 529/531 (99.62%), Query Frame = 0

Query: 1   MPSSSIVFRWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSSSIVF+WLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF
Sbjct: 1   MPSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60

Query: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICW 120
           ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITN FHSPSYWLIFFLTVLAGNSICW
Sbjct: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180
           INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN
Sbjct: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180

Query: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240
           SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL
Sbjct: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240

Query: 241 SGLVGIMVFLVLPLVVVVEEKVKERVEKGKEGRVYHFTVEENNDVERIMRGENERKVERT 300
           SGLVGIMVFLVLPLVVVV EKVKERVE+GKEGRVYHFTVEENNDVERIMRGENERKVERT
Sbjct: 241 SGLVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERT 300

Query: 301 DDGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
           D+GEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS
Sbjct: 301 DEGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360

Query: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI 420
           SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI
Sbjct: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI 420

Query: 421 IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480
           IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA
Sbjct: 421 IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480

Query: 481 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 531
           AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN
Sbjct: 481 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 531

BLAST of MELO3C020496.jh1 vs. NCBI nr
Match: XP_011658866.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN65862.1 hypothetical protein Csa_023247 [Cucumis sativus])

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 504/532 (94.74%), Postives = 512/532 (96.24%), Query Frame = 0

Query: 1   MPSSS-IVFRWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL 60
           MPSSS IVF+WLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL
Sbjct: 1   MPSSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL 60

Query: 61  FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSIC 120
           FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITN FHSPSYWLIFFLTVLAGNSIC
Sbjct: 61  FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSIC 120

Query: 121 WINTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLL 180
           WINTVCYMVAITNF SS+RQVAVGISTSYQGLSAKVYTDIVGSIFSNK SSKTAETFLLL
Sbjct: 121 WINTVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLL 180

Query: 181 NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSA 240
           NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATG FAT+TSVGSVSRMLSA
Sbjct: 181 NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSA 240

Query: 241 LSGLVGIMVFLVLPLVVVVEEKVKERVEKGKEGRVYHFTVEENNDVERIMRGENERKVER 300
           L GLVGIMVFLVLPLVVVV EKVKERVE+GKEG+VYHFTVEE ND ER MRGENERKVER
Sbjct: 241 LGGLVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEER-MRGENERKVER 300

Query: 301 TDDGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSL 360
           TDDGEAMEEIGAKEMVKRINFWLY GVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSL
Sbjct: 301 TDDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSL 360

Query: 361 SSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTA 420
           SSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMG LCGGFFLLLSPSDTSLCMSTA
Sbjct: 361 SSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTA 420

Query: 421 IIAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGV 480
           IIAICTGAITSISVSTTTDLFGATNFSINHN+VVANIPFGSFIFGYMAAFLY KQAGHGV
Sbjct: 421 IIAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYRKQAGHGV 480

Query: 481 AAGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 531
             GKCIGVECYRTTFLIWG FSSFGTFLALLLFART+SFYSTT  SS+I  N
Sbjct: 481 DPGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIPSN 531

BLAST of MELO3C020496.jh1 vs. NCBI nr
Match: KAA0059993.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ97252.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 942 bits (2434), Expect = 0.0
Identity = 502/531 (94.54%), Postives = 505/531 (95.10%), Query Frame = 0

Query: 1   MPSSSIVFRWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSSSIVF+WLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF
Sbjct: 1   MPSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60

Query: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICW 120
           ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITN FHSPSYWLIFFLTVLAGNSICW
Sbjct: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180
           INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN
Sbjct: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180

Query: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240
           SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL
Sbjct: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240

Query: 241 SGLVGIMVFLVLPLVVVVEEKVKERVEKGKEGRVYHFTVEENNDVERIMRGENERKVERT 300
           SGLVGIMVFLVLPLVVVV EKVKERVE+GKEGRVYHFTVEENNDVERIMRGENERKVERT
Sbjct: 241 SGLVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERT 300

Query: 301 DDGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
           D+GEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS
Sbjct: 301 DEGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360

Query: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI 420
           SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGG                  
Sbjct: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGG------------------ 420

Query: 421 IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480
                 AITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA
Sbjct: 421 ------AITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480

Query: 481 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 531
           AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN
Sbjct: 481 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 507

BLAST of MELO3C020496.jh1 vs. NCBI nr
Match: XP_038895111.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Benincasa hispida])

HSP 1 Score: 837 bits (2162), Expect = 1.83e-302
Identity = 451/528 (85.42%), Postives = 485/528 (91.86%), Query Frame = 0

Query: 3   SSSIVFRWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 62
           SSSIVF+WLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC
Sbjct: 2   SSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 61

Query: 63  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICWIN 122
           FSGLAANYLPLWLVLFIG+SLGL+GYGVQYLF+TN   SPSY +IF LTVLAGNSICWIN
Sbjct: 62  FSGLAANYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWIN 121

Query: 123 TVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSV 182
           TVCYMVAITNF + NRQ+AVGISTSYQGLSAK+YT+IV SIFS K SSKT ETFLLLNSV
Sbjct: 122 TVCYMVAITNFGT-NRQIAVGISTSYQGLSAKIYTNIVSSIFS-KHSSKTTETFLLLNSV 181

Query: 183 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSG 242
           LP+GVCVL SPLAR +K+VEEQGKLEVGFFVIFVITIATG FA +TSVGS+SRMLS LSG
Sbjct: 182 LPMGVCVLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSG 241

Query: 243 LVGIMVFLVLPLVVVVEEKVKERVEKGKEGRVYHFTVEENNDVERIMRGENERKVERTDD 302
           LVGI+VFLVLPLVV V EKVKE +++GKE RVYHFTVEEN DVER+ RGEN+ K+ER  D
Sbjct: 242 LVGIVVFLVLPLVVAVVEKVKESLKEGKERRVYHFTVEENKDVERV-RGENQSKLERDGD 301

Query: 303 GE-AMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSS 362
            E AMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSS
Sbjct: 302 SEEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSS 361

Query: 363 SFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAII 422
           SFGFFGRL+PSILDYFLSR+ K MKSKPGWMVGLMG LCGGFFLLL P+D SLC+STAII
Sbjct: 362 SFGFFGRLMPSILDYFLSRK-KCMKSKPGWMVGLMGVLCGGFFLLLCPTDVSLCVSTAII 421

Query: 423 AICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAA 482
           A+CTGAITSISVSTT++LFGA NFSINHN++VANIPFGSF+FGYMAAFLYHKQAG G   
Sbjct: 422 AVCTGAITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQAG-GDGT 481

Query: 483 GKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHIS 529
           GKCIGVECYRTTF IWGCFSSFGT LALLLFART++FYSTT   SH S
Sbjct: 482 GKCIGVECYRTTFFIWGCFSSFGTLLALLLFARTKTFYSTTR--SHTS 522

BLAST of MELO3C020496.jh1 vs. NCBI nr
Match: XP_022970645.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 775 bits (2001), Expect = 5.20e-278
Identity = 421/522 (80.65%), Postives = 457/522 (87.55%), Query Frame = 0

Query: 1   MPSSSIVFRWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSS  VF+WLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLF
Sbjct: 1   MPSS--VFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLF 60

Query: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICW 120
           AC SGLAANYLPLW VL IGSSLGL+GYGVQYLFITN FHSPSY LIFFLTVLAGNSICW
Sbjct: 61  ACVSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180
           INTVCY+VAI NF S NRQVAVGISTSYQGLSAK+YTDIVG IFS K S KTAETFLL N
Sbjct: 121 INTVCYIVAIRNFPS-NRQVAVGISTSYQGLSAKIYTDIVGLIFS-KRSDKTAETFLLSN 180

Query: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240
           SV PLGVCVLVSP+AR V++VEEQG LEVGFFVIFVITIATG FA +TSVGS+SRMLS L
Sbjct: 181 SVFPLGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPL 240

Query: 241 SGLVGIMVFLVLPLVVVVEEKVKERVEKGKEGRVYHFTVEENNDVERIMRGENERKVERT 300
           SGLVGI+VFL+LP+VV V E VK+ V +GKE RVYH T+EE+  +    R ENE K+ R 
Sbjct: 241 SGLVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRI----RPENEAKLNRD 300

Query: 301 DDGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
              EAMEEIGA EM+KR+NFWLY GVY FGATLGLAFLNNLGQIAESRGS SVSSLVSLS
Sbjct: 301 GGDEAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLS 360

Query: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI 420
           SSFGFFGRL+PS+LD+FLSRR K MKSKPGW+V LMGA+CGGFFLL  P+D  LC+STA+
Sbjct: 361 SSFGFFGRLMPSLLDHFLSRR-KMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAV 420

Query: 421 IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480
           IA+CTGAITSISVSTT++LFGA NFSINHNVVVANIP GSF+FGYMAA+LY KQA  G  
Sbjct: 421 IAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGG-- 480

Query: 481 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTT 522
           AGKCIGVECYRTTF+IWGC  SFGT LALLLFARTRSFYSTT
Sbjct: 481 AGKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTT 511

BLAST of MELO3C020496.jh1 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.1e-72
Identity = 187/531 (35.22%), Postives = 290/531 (54.61%), Query Frame = 0

Query: 9   RWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 68
           +W  L+  IW+Q+  GTNF+F +YSS LK  L +SQ++LN LA ASD GK F   SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 69  NYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICWINTVCYMV 128
            Y PL +VLF  +++G +GYGVQ+L ITN    P Y L+F   +LAG SICW NT C+++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 129 AITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVLPLGVC 188
            I +F  +NR +A+ ++ S+ G+SA +Y     S+  N  +  ++  +LLLNS++PL V 
Sbjct: 163 CIRHF-PNNRALALSLTVSFNGISAALY-----SLAFNAINPSSSNLYLLLNSLVPLVVS 222

Query: 189 ------VLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSG 248
                 VL  P        + +      F ++ V+ + T     ++S  + S  L+ +  
Sbjct: 223 FAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGA 282

Query: 249 LVGIMVFLVLPLVVVVEE----KVKERVEKGKEGRVYHFTVEENNDVERIMRGENERKVE 308
           +V ++  L  PL+V   +     +  R+     G V     E  N    +        + 
Sbjct: 283 VVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMG 342

Query: 309 RTDDGEAM---EEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSS 368
              +G  +   +E   + ++ R+ FWLY+  Y  G T+GL + NNLGQIA+S G +S ++
Sbjct: 343 TAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS-TT 402

Query: 369 LVSLSSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLL--SPSDTS 428
           LV++ SSF FFGRLL +  D+      +F  ++ GW    +      FFLL   S   T+
Sbjct: 403 LVTIYSSFSFFGRLLSAAPDFM---HKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTA 462

Query: 429 LCMSTAIIAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHK 488
           L  +TA+I + +G I + +VS T+DLFG  +  +NHN+++ NIP GS ++GY+AA +Y  
Sbjct: 463 LQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIYEA 522

Query: 489 QAGHGVA-----AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFY 520
            A   +      +  CIG +CY  TF+ WGC S  G   +L L+ RT+  Y
Sbjct: 523 NASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of MELO3C020496.jh1 vs. ExPASy TrEMBL
Match: A0A1S3C569 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497028 PE=4 SV=1)

HSP 1 Score: 999 bits (2583), Expect = 0.0
Identity = 526/531 (99.06%), Postives = 529/531 (99.62%), Query Frame = 0

Query: 1   MPSSSIVFRWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSSSIVF+WLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF
Sbjct: 1   MPSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60

Query: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICW 120
           ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITN FHSPSYWLIFFLTVLAGNSICW
Sbjct: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180
           INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN
Sbjct: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180

Query: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240
           SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL
Sbjct: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240

Query: 241 SGLVGIMVFLVLPLVVVVEEKVKERVEKGKEGRVYHFTVEENNDVERIMRGENERKVERT 300
           SGLVGIMVFLVLPLVVVV EKVKERVE+GKEGRVYHFTVEENNDVERIMRGENERKVERT
Sbjct: 241 SGLVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERT 300

Query: 301 DDGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
           D+GEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS
Sbjct: 301 DEGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360

Query: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI 420
           SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI
Sbjct: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI 420

Query: 421 IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480
           IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA
Sbjct: 421 IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480

Query: 481 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 531
           AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN
Sbjct: 481 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 531

BLAST of MELO3C020496.jh1 vs. ExPASy TrEMBL
Match: A0A0A0LYD4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G533690 PE=4 SV=1)

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 504/532 (94.74%), Postives = 512/532 (96.24%), Query Frame = 0

Query: 1   MPSSS-IVFRWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL 60
           MPSSS IVF+WLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL
Sbjct: 1   MPSSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL 60

Query: 61  FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSIC 120
           FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITN FHSPSYWLIFFLTVLAGNSIC
Sbjct: 61  FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSIC 120

Query: 121 WINTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLL 180
           WINTVCYMVAITNF SS+RQVAVGISTSYQGLSAKVYTDIVGSIFSNK SSKTAETFLLL
Sbjct: 121 WINTVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLL 180

Query: 181 NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSA 240
           NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATG FAT+TSVGSVSRMLSA
Sbjct: 181 NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSA 240

Query: 241 LSGLVGIMVFLVLPLVVVVEEKVKERVEKGKEGRVYHFTVEENNDVERIMRGENERKVER 300
           L GLVGIMVFLVLPLVVVV EKVKERVE+GKEG+VYHFTVEE ND ER MRGENERKVER
Sbjct: 241 LGGLVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEER-MRGENERKVER 300

Query: 301 TDDGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSL 360
           TDDGEAMEEIGAKEMVKRINFWLY GVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSL
Sbjct: 301 TDDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSL 360

Query: 361 SSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTA 420
           SSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMG LCGGFFLLLSPSDTSLCMSTA
Sbjct: 361 SSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTA 420

Query: 421 IIAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGV 480
           IIAICTGAITSISVSTTTDLFGATNFSINHN+VVANIPFGSFIFGYMAAFLY KQAGHGV
Sbjct: 421 IIAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYRKQAGHGV 480

Query: 481 AAGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 531
             GKCIGVECYRTTFLIWG FSSFGTFLALLLFART+SFYSTT  SS+I  N
Sbjct: 481 DPGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIPSN 531

BLAST of MELO3C020496.jh1 vs. ExPASy TrEMBL
Match: A0A5D3BBQ0 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00460 PE=4 SV=1)

HSP 1 Score: 942 bits (2434), Expect = 0.0
Identity = 502/531 (94.54%), Postives = 505/531 (95.10%), Query Frame = 0

Query: 1   MPSSSIVFRWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSSSIVF+WLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF
Sbjct: 1   MPSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60

Query: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICW 120
           ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITN FHSPSYWLIFFLTVLAGNSICW
Sbjct: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180
           INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN
Sbjct: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180

Query: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240
           SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL
Sbjct: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240

Query: 241 SGLVGIMVFLVLPLVVVVEEKVKERVEKGKEGRVYHFTVEENNDVERIMRGENERKVERT 300
           SGLVGIMVFLVLPLVVVV EKVKERVE+GKEGRVYHFTVEENNDVERIMRGENERKVERT
Sbjct: 241 SGLVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERT 300

Query: 301 DDGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
           D+GEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS
Sbjct: 301 DEGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360

Query: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI 420
           SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGG                  
Sbjct: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGG------------------ 420

Query: 421 IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480
                 AITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA
Sbjct: 421 ------AITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480

Query: 481 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 531
           AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN
Sbjct: 481 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 507

BLAST of MELO3C020496.jh1 vs. ExPASy TrEMBL
Match: A0A6J1I3F0 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469568 PE=4 SV=1)

HSP 1 Score: 775 bits (2001), Expect = 2.52e-278
Identity = 421/522 (80.65%), Postives = 457/522 (87.55%), Query Frame = 0

Query: 1   MPSSSIVFRWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSS  VF+WLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLF
Sbjct: 1   MPSS--VFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLF 60

Query: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICW 120
           AC SGLAANYLPLW VL IGSSLGL+GYGVQYLFITN FHSPSY LIFFLTVLAGNSICW
Sbjct: 61  ACVSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180
           INTVCY+VAI NF S NRQVAVGISTSYQGLSAK+YTDIVG IFS K S KTAETFLL N
Sbjct: 121 INTVCYIVAIRNFPS-NRQVAVGISTSYQGLSAKIYTDIVGLIFS-KRSDKTAETFLLSN 180

Query: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240
           SV PLGVCVLVSP+AR V++VEEQG LEVGFFVIFVITIATG FA +TSVGS+SRMLS L
Sbjct: 181 SVFPLGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPL 240

Query: 241 SGLVGIMVFLVLPLVVVVEEKVKERVEKGKEGRVYHFTVEENNDVERIMRGENERKVERT 300
           SGLVGI+VFL+LP+VV V E VK+ V +GKE RVYH T+EE+  +    R ENE K+ R 
Sbjct: 241 SGLVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRI----RPENEAKLNRD 300

Query: 301 DDGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
              EAMEEIGA EM+KR+NFWLY GVY FGATLGLAFLNNLGQIAESRGS SVSSLVSLS
Sbjct: 301 GGDEAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLS 360

Query: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI 420
           SSFGFFGRL+PS+LD+FLSRR K MKSKPGW+V LMGA+CGGFFLL  P+D  LC+STA+
Sbjct: 361 SSFGFFGRLMPSLLDHFLSRR-KMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAV 420

Query: 421 IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480
           IA+CTGAITSISVSTT++LFGA NFSINHNVVVANIP GSF+FGYMAA+LY KQA  G  
Sbjct: 421 IAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGG-- 480

Query: 481 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTT 522
           AGKCIGVECYRTTF+IWGC  SFGT LALLLFARTRSFYSTT
Sbjct: 481 AGKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTT 511

BLAST of MELO3C020496.jh1 vs. ExPASy TrEMBL
Match: A0A6J1HMC6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464885 PE=4 SV=1)

HSP 1 Score: 755 bits (1950), Expect = 1.08e-269
Identity = 413/523 (78.97%), Postives = 452/523 (86.42%), Query Frame = 0

Query: 1   MPSSSIVFRWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSS  VF+WLSL+GIIWLQSINGTNFNFPSYSS LK  LS+SQ QLNNLAFASDAGKLF
Sbjct: 55  MPSS--VFQWLSLLGIIWLQSINGTNFNFPSYSSPLKHHLSISQFQLNNLAFASDAGKLF 114

Query: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICW 120
           AC SGLAANYLPLW +L IGS LGL+GYGVQYLFITN FHSPSY LIFFL+VLAGNSICW
Sbjct: 115 ACVSGLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLSVLAGNSICW 174

Query: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180
           INTVCY+VAI NF S NRQVAVGISTSYQGLSAK+YTDIVG IFS K S KTAETFLL N
Sbjct: 175 INTVCYIVAIRNFPS-NRQVAVGISTSYQGLSAKIYTDIVGLIFS-KRSDKTAETFLLSN 234

Query: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240
           SV PLGVCVLV P+AR V++VEE+G LEVGFFVIFVITIATG FA +TSVGS+SRMLS L
Sbjct: 235 SVFPLGVCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPL 294

Query: 241 SGLVGIMVFLVLPLVVVVEEKVKERVEKGKEGRVYHFTVEENNDVERIMRGENERKVERT 300
           SGLVGI+VFL+LP+VV V E VK+ V +GKE RVYH T+EE+  +    R ENE K+ R 
Sbjct: 295 SGLVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRI----RPENEAKLNRD 354

Query: 301 DDGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
              EAMEEIGA EM+KR+NFWLY GVY FGATLGLAFLNNLGQIAES GS SVSSLVSLS
Sbjct: 355 GGDEAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESWGSESVSSLVSLS 414

Query: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLS-PSDTSLCMSTA 420
           SSFGFFGRL+PS+LD+FLSRR K MKSKPGW+V LMGA+CGGFF LLS P+D  LC+STA
Sbjct: 415 SSFGFFGRLMPSLLDHFLSRR-KMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVSTA 474

Query: 421 IIAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGV 480
           +IA+CTGAITSISVSTT++LFGA NFSINHNVVVANIPFGSF+FGYMAA+LY KQA    
Sbjct: 475 VIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATGDT 534

Query: 481 AAGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTT 522
             GKCIGVECYRTTF+IWGC  S GT LALLLFARTRSFYSTT
Sbjct: 535 --GKCIGVECYRTTFMIWGCLCSLGTLLALLLFARTRSFYSTT 566

BLAST of MELO3C020496.jh1 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 409.5 bits (1051), Expect = 4.2e-114
Identity = 252/526 (47.91%), Postives = 333/526 (63.31%), Query Frame = 0

Query: 7   VFRWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGL 66
           +  WLSL+ I+WLQS NGTN +FP+YSSQLK+ L +SQ +LN L+FASDAGK+    SG+
Sbjct: 5   ILPWLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGFISGI 64

Query: 67  AANYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICWINTVCY 126
           AA YLPL LVL  G SLG  GYG+QYL I                      ICWINT CY
Sbjct: 65  AAVYLPLPLVLLAGGSLGFAGYGLQYLSIIKKI------------------ICWINTACY 124

Query: 127 MVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVLPLG 186
           +VAI +F   NRQVAVGI+ SYQGLS K+YTD+V S F +    + A  +LLLNS++PL 
Sbjct: 125 IVAINSF-PVNRQVAVGITASYQGLSGKIYTDMVHSFF-HTSQREEASGYLLLNSLVPLV 184

Query: 187 VCVLVSPLAR---FVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSGL 246
            C++ +P+       K +     ++VGF V+FV+TIATG +A  TS+ SV  +L     L
Sbjct: 185 ACLVTAPMLMRHGGDKTMSYSKDVKVGFIVLFVLTIATGIYAVATSLVSVPAVLV----L 244

Query: 247 VGIMVFLVLPLVVVVEEKVKERVEKGKEGRVYH---------FTVEENNDVERIMRGENE 306
           VGI +FL+ PL + +    KE +   K  +  H         + VEE++  E       E
Sbjct: 245 VGIALFLLAPLAIPIGVGFKELMSSRKTQQKVHDLEAPVDKFYFVEEDHTKE-------E 304

Query: 307 RKVERTDDGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVS 366
            + E+   G   EE+   ++ K+++FW+YFG+YLFG T+GL F NNLGQIAESRGS++ S
Sbjct: 305 EEFEKAIIG-VKEEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNLGQIAESRGSTATS 364

Query: 367 SLVSLSSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSL 426
           SLV+LSSSFGFFGRLLPS+LDYF S RNK+M S P  M G + A+   F LLL  SD +L
Sbjct: 365 SLVALSSSFGFFGRLLPSLLDYFFS-RNKYMPSSPVSMAGSLVAMVASFLLLLIDSDIAL 424

Query: 427 CMSTAIIAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQ 486
            + TA+I I +GA+TS+SV+ T +LFG  +F +NHN+VV +IP GSF FG +AA +Y   
Sbjct: 425 YIGTAMIGIFSGALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGSFSFGLLAAKIYRDG 484

Query: 487 AGHGVAAGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYS 521
           A      GKC G+ C++TT + WG   S    LA +L+ R R FYS
Sbjct: 485 AALYGDDGKCFGMHCFQTTLVFWGMLCSIAALLAAVLYIRNRKFYS 497

BLAST of MELO3C020496.jh1 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 275.8 bits (704), Expect = 7.3e-74
Identity = 200/561 (35.65%), Postives = 303/561 (54.01%), Query Frame = 0

Query: 9   RWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 68
           +W+ L+  IW+Q+  GTNF+F +YSS LK  L +SQ+QLN LA ASD GK+F   SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 69  NYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICWINTVCYMV 128
            Y PLW VLF  + +G +GYGVQ+L ITN    P Y L+F   +LAG SICW NTVC+++
Sbjct: 67  LYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLP-YILVFLCCLLAGLSICWFNTVCFVL 126

Query: 129 AITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVLPLGVC 188
            I NF  +NR +A+ ++ S+ G+SA +YT     +  N  +  + E +LLLN+++PL V 
Sbjct: 127 CIRNF-PANRSLALSLTVSFNGVSAALYT-----LAYNAINPVSTELYLLLNALVPLFVS 186

Query: 189 -VLVSPLARFVKI----VEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSGL 248
              + P+ R   +     +   +  + F ++ ++ +  G +  +      S + SA    
Sbjct: 187 FAALIPILRQPPLEPLPPDGVRRDSLMFLLLNILAVLNGVYLLLFR-SKTSDVTSARLLF 246

Query: 249 VGIMVFLVLPL----------------------------VVVVEEKVKERVEKGKEGRVY 308
            G ++ L+LPL                            +V V+E    +    +E  + 
Sbjct: 247 GGSILLLILPLCLPGLVYARNWYLHNIHSSFRLEGSGFILVDVDELEMHKGMVTREASLE 306

Query: 309 HFTVEENNDVERIMRGENERKVERTDDGEAM-------------EEIGAKEMVKRINFWL 368
            + +  N+DV R +   +++     DDG                EE     ++ R +FWL
Sbjct: 307 GYQL-LNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRSDFWL 366

Query: 369 YFGVYLFGATLGLAFLNNLGQIAESRG-SSSVSSLVSLSSSFGFFGRLLPSILDYFLSRR 428
           Y+  Y  G T+GL + NNLGQIA+S G SS  ++LV+L SSF FFGRLL +  DY    R
Sbjct: 367 YYIAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYI---R 426

Query: 429 NKFMKSKPGWM-VGLMGALCGGFFLLLSPSDTSLCMSTAIIAICTGAITSISVSTTTDLF 488
            K   ++ GW+ V L+      F L  S S  +L   TA+I + +G I + +VS T++LF
Sbjct: 427 AKVYFARTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSELF 486

Query: 489 GATNFSINHNVVVANIPFGSFIFGYMAAFLY--HKQAGHGVAAGKCIGVECYRTTFLIWG 520
           G  +  +NHN+++ NIP GS ++G++AA +Y  H  AG    +  C+G +CY  TF+ WG
Sbjct: 487 GPNSVGVNHNILITNIPIGSLVYGFLAALVYESHSVAGSKTESVICMGRDCYLQTFMWWG 546

BLAST of MELO3C020496.jh1 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 273.5 bits (698), Expect = 3.6e-73
Identity = 187/531 (35.22%), Postives = 290/531 (54.61%), Query Frame = 0

Query: 9   RWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 68
           +W  L+  IW+Q+  GTNF+F +YSS LK  L +SQ++LN LA ASD GK F   SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 69  NYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICWINTVCYMV 128
            Y PL +VLF  +++G +GYGVQ+L ITN    P Y L+F   +LAG SICW NT C+++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 129 AITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVLPLGVC 188
            I +F  +NR +A+ ++ S+ G+SA +Y     S+  N  +  ++  +LLLNS++PL V 
Sbjct: 163 CIRHF-PNNRALALSLTVSFNGISAALY-----SLAFNAINPSSSNLYLLLNSLVPLVVS 222

Query: 189 ------VLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSG 248
                 VL  P        + +      F ++ V+ + T     ++S  + S  L+ +  
Sbjct: 223 FAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGA 282

Query: 249 LVGIMVFLVLPLVVVVEE----KVKERVEKGKEGRVYHFTVEENNDVERIMRGENERKVE 308
           +V ++  L  PL+V   +     +  R+     G V     E  N    +        + 
Sbjct: 283 VVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMG 342

Query: 309 RTDDGEAM---EEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSS 368
              +G  +   +E   + ++ R+ FWLY+  Y  G T+GL + NNLGQIA+S G +S ++
Sbjct: 343 TAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS-TT 402

Query: 369 LVSLSSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLL--SPSDTS 428
           LV++ SSF FFGRLL +  D+      +F  ++ GW    +      FFLL   S   T+
Sbjct: 403 LVTIYSSFSFFGRLLSAAPDFM---HKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTA 462

Query: 429 LCMSTAIIAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHK 488
           L  +TA+I + +G I + +VS T+DLFG  +  +NHN+++ NIP GS ++GY+AA +Y  
Sbjct: 463 LQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIYEA 522

Query: 489 QAGHGVA-----AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFY 520
            A   +      +  CIG +CY  TF+ WGC S  G   +L L+ RT+  Y
Sbjct: 523 NASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of MELO3C020496.jh1 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 2.6e-71
Identity = 201/576 (34.90%), Postives = 312/576 (54.17%), Query Frame = 0

Query: 9   RWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 68
           +W+ L+  IW+Q+  GTNF+F +YSS LK  L +SQ+QLN LA ASD GK+F   SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 69  NYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICWINTVCYMV 128
            Y PLW VLF  + +G +GYGVQ+L IT HF S  Y ++F   +LAG SICW NTVC+++
Sbjct: 67  MYFPLWTVLFAAAFMGFVGYGVQWLVIT-HFISLPYIMVFLCCLLAGLSICWFNTVCFVL 126

Query: 129 AITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVLPLGVC 188
            I+NF  +NR +A+ ++ S+ G+SA +YT     +  N  +  + E +LLLN+++PL V 
Sbjct: 127 CISNF-PANRSLALSLTVSFNGVSAALYT-----LAYNAINPTSPELYLLLNALIPLIVS 186

Query: 189 -VLVSPLAR---FVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSA---LS 248
              + P+ R   F  +  +  + +   F++  I  A      +    + S + SA     
Sbjct: 187 FTAIIPILRQPPFEPLPPDGVRRDSLMFLLLNILAALNGVYLLLFGSNSSDLTSARLLFG 246

Query: 249 GLVGIMVF-LVLPLVVVVEEKVKERVEKGKEGRVYHFTVEENNDVE---RIMRGENERK- 308
           G + ++VF L +P +V+        +          F + + +++E    ++  E  R+ 
Sbjct: 247 GAILLLVFPLCIPGLVIARNWYNRTIHTSFRLEGSGFILVDPDELELHKGMLAHEANREG 306

Query: 309 -----------------VERTDDGEA----------MEEIGAKE----MVKRINFWLYFG 368
                            VE  D  E+          +E +G +     ++ R +FWLY+ 
Sbjct: 307 YQLLSDDVVQNPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSDFWLYYI 366

Query: 369 VYLFGATLGLAFLNNLGQIAESRG-SSSVSSLVSLSSSFGFFGRLLPSILDYFLSRRNKF 428
            Y  G T+GL + NNLGQIA+S G SS+ ++LV+L S+F FFGRLL +  DY    R K 
Sbjct: 367 TYFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYI---RAKV 426

Query: 429 MKSKPGWM-VGLMGALCGGFFLLLSPSDTSLCMSTAIIAICTGAITSISVSTTTDLFGAT 488
             ++ GW+ + L+      F L  S + ++L   TA++ + +G I + +VS T++LFG  
Sbjct: 427 YFARTGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELFGPN 486

Query: 489 NFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAAGK--------CIGVECYRTTFL 532
           +  +NHN+++ NIP GS I+G++AA +Y     HG    K        C+G +CY  TF+
Sbjct: 487 SVGVNHNILITNIPIGSLIYGFLAALVYD---SHGFTGTKSMTSESVVCMGRDCYYLTFV 546

BLAST of MELO3C020496.jh1 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 6.6e-67
Identity = 188/539 (34.88%), Postives = 292/539 (54.17%), Query Frame = 0

Query: 9   RWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 68
           RW  L+  IW+Q+  GTNF+F +YSS +K  + +SQ +LN +A ASD GK     SG A 
Sbjct: 23  RWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSRLNYMAVASDLGKALGWSSGFAI 82

Query: 69  NYLPLWLVLFIGSSLGLIGYGVQYLFITNHFHSPSYWLIFFLTVLAGNSICWINTVCYMV 128
            Y P+  VLF  +++GL+GYGVQ+L I +    P Y L+     LAG SICW NTV +++
Sbjct: 83  AYFPVSGVLFAAAAMGLVGYGVQWLSIADVIDLP-YSLVLVCCSLAGLSICWFNTVSFIL 142

Query: 129 AITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVLPLGVC 188
            I +F  +N  +A+ +  S+ G+SA +YT  +G    +  SS +++ +LLLNS++PL V 
Sbjct: 143 CIRHF-KANHSLALSLVVSFNGISAALYT--LGHEAISGKSSASSDIYLLLNSLIPLIVS 202

Query: 189 ------VLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSG 248
                 VL +P +          +  + F V  V+ + T  +  + S G+     S    
Sbjct: 203 VLALWPVLTNPNSSETDTTRTHDETRI-FVVFNVLALVTCFYLLLPSSGTYLAS-SPRWH 262

Query: 249 LVGIMVFLVLPLVVVVEEKVKERVEKGKEGRVYHFT---VEENNDVERIMRGENERK--V 308
            +G +  L+ PL V       + + +  E   +H +      N +  +I++ ++++    
Sbjct: 263 FLGAIFLLLFPLCV----PFLDYIHRALESCFHHHSSGYAAVNIEEPKILKIKSQKSNAE 322

Query: 309 ERTDDGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRG--SSSVSS 368
           E +D     +E     +V+++ FWLY+  Y  G T+GL + NNLGQIA+S G  SS+  S
Sbjct: 323 EESDQVRLGDEHSLGMLVRKLEFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGQSSSNAKS 382

Query: 369 LVSLSSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWM-VGLMGALCGGFFLLLSPSDTS- 428
           LV+L S+F F GRLL S  D F  ++ K++ ++ GW  + L+      F L  SP     
Sbjct: 383 LVTLFSAFSFLGRLLSSAPD-FTRKKLKYL-TRTGWFTISLLPTPLAFFILAYSPKTNQT 442

Query: 429 --LCMSTAIIAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLY 488
             L ++TA+I + +G + + +VS T++LFG  +  +N N+++ NIP GS  +GYMA  +Y
Sbjct: 443 ALLEVATALIGLSSGFVFAAAVSITSELFGRNSVGVNQNILITNIPIGSLFYGYMAGSVY 502

Query: 489 HKQAGHG---VAAGK--CIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLS 526
              A  G   V A    C+G +CY  TFL WGC S  G   +L LF RTR  Y    L+
Sbjct: 503 DTNASLGRKSVVADSVVCVGRKCYFVTFLFWGCLSVLGFVCSLFLFIRTRPVYHRLKLN 549