Homology
BLAST of MELO3C020052.jh1 vs. NCBI nr
Match:
XP_008456865.1 (PREDICTED: helicase SEN1-like [Cucumis melo])
HSP 1 Score: 1691 bits (4378), Expect = 0.0
Identity = 837/848 (98.70%), Postives = 843/848 (99.41%), Query Frame = 0
Query: 1 MEGEGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYK 60
MEGEGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYK
Sbjct: 1 MEGEGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYK 60
Query: 61 ASYIFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILFDVNVSSWRNSDGGKG 120
ASYIFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILF+VNVSSWRN DGGKG
Sbjct: 61 ASYIFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILFNVNVSSWRNRDGGKG 120
Query: 121 QQPYKSLPGDIFVILDADPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNM 180
QQPYKSLPGDIFVILD DPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNM
Sbjct: 121 QQPYKSLPGDIFVILDTDPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNM 180
Query: 181 DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDNKTCKQCNGNDGEDY 240
DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDNKTCKQCNGNDGEDY
Sbjct: 181 DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDNKTCKQCNGNDGEDY 240
Query: 241 STQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWKI 300
STQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWKI
Sbjct: 241 STQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWKI 300
Query: 301 LTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFDS 360
LTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFDS
Sbjct: 301 LTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFDS 360
Query: 361 HDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDCVH 420
HDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDCVH
Sbjct: 361 HDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDCVH 420
Query: 421 IFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDDDDKMGEALIDLKCHCLL 480
IFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDD KMGEALIDLKCHCLL
Sbjct: 421 IFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDD--KMGEALIDLKCHCLL 480
Query: 481 VLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVDEA 540
VLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVDEA
Sbjct: 481 VLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVDEA 540
Query: 541 AQLKECESLIPLQLQHINHALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSKHL 600
AQLKECESL+PLQLQHI HALLVGDEFQLPATIKSK+CEGAKFGRSLYERLSLMGYSKHL
Sbjct: 541 AQLKECESLVPLQLQHITHALLVGDEFQLPATIKSKICEGAKFGRSLYERLSLMGYSKHL 600
Query: 601 LDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEES 660
LDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEES
Sbjct: 601 LDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEES 660
Query: 661 NGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEKN 720
NGDGQSKKNTVEVIVVTQI+QMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEKN
Sbjct: 661 NGDGQSKKNTVEVIVVTQIIQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEKN 720
Query: 721 NNEGFRVKVKSIDGFQGGEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLWIVG 780
NNEGFRVKVKSIDGFQGGEEDVIIISTVRSN+GHNIGFLSNKQRTNVALTRARFCLWIVG
Sbjct: 721 NNEGFRVKVKSIDGFQGGEEDVIIISTVRSNNGHNIGFLSNKQRTNVALTRARFCLWIVG 780
Query: 781 DAKTLGKSNSEWRDVINDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLDNIY 840
DAKTLGKSNSEWRD+INDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLDNIY
Sbjct: 781 DAKTLGKSNSEWRDIINDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLDNIY 840
Query: 841 NNNHYGRV 848
NNNHYGRV
Sbjct: 841 NNNHYGRV 846
BLAST of MELO3C020052.jh1 vs. NCBI nr
Match:
KGN50687.2 (hypothetical protein Csa_004741 [Cucumis sativus])
HSP 1 Score: 1405 bits (3637), Expect = 0.0
Identity = 719/850 (84.59%), Postives = 754/850 (88.71%), Query Frame = 0
Query: 1 MEGEGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYK 60
ME EGGLS NK SNHK+SNGLIDALFSWDFNNVFNQN YK KVRKIPKSFETEEQYK
Sbjct: 1 MEAEGGLS----NKGSNHKDSNGLIDALFSWDFNNVFNQNIYKPKVRKIPKSFETEEQYK 60
Query: 61 ASYIFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILFDVNVSSWRNSDGGKG 120
SYIFPLLEETRAELCSNLKTIQKAPFSQVISIE++NTKK KILF+VNVSSWRN+ GGKG
Sbjct: 61 GSYIFPLLEETRAELCSNLKTIQKAPFSQVISIETSNTKKDKILFNVNVSSWRNTYGGKG 120
Query: 121 QQPYKSLPGDIFVILDADPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNM 180
QQPYKSLPGD FVILD DPQTITSDYLEKSSKLNWAFAWLGQ+NDNNTPTHL LHISN+M
Sbjct: 121 QQPYKSLPGDFFVILDVDPQTITSDYLEKSSKLNWAFAWLGQVNDNNTPTHLKLHISNSM 180
Query: 181 DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDNKTCKQCNGN-DGED 240
DQL STPLFIVFLMNLTTNLRIWKTLQCSS GGIVKH+LGT S+DNKTCKQCN DGED
Sbjct: 181 DQLKSTPLFIVFLMNLTTNLRIWKTLQCSSSGGIVKHVLGTMSMDNKTCKQCNNQTDGED 240
Query: 241 YSTQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWK 300
ST++ TLRL SLSLNESQRVAIESCI+KV CQHKPSIELIWGPPGTGKTKTTSILLWK
Sbjct: 241 -STEDMATLRLASLSLNESQRVAIESCIRKVKCQHKPSIELIWGPPGTGKTKTTSILLWK 300
Query: 301 ILTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFD 360
IL INHQIRTLACAPTNVAITNLASQV+ LLKHDSLS+N +FCPLGELLLFGNKDRLKFD
Sbjct: 301 ILAINHQIRTLACAPTNVAITNLASQVLKLLKHDSLSRNAIFCPLGELLLFGNKDRLKFD 360
Query: 361 SHDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDCV 420
QL+DIYLDRRVEKLFKCLGQ+GLKFQI+SMIGIFQENKLSK+KRMFK N S LL+CV
Sbjct: 361 YSHQLEDIYLDRRVEKLFKCLGQYGLKFQISSMIGIFQENKLSKLKRMFKSNVSSLLECV 420
Query: 421 HIFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDDDDKMGEALIDLKCHCL 480
HIFTTHIP+QVIMEHNWKKLEILVGFICDIGTLLSK+NY+ DDDD MGEALIDLKCH L
Sbjct: 421 HIFTTHIPQQVIMEHNWKKLEILVGFICDIGTLLSKNNYNYDDDD-TMGEALIDLKCHFL 480
Query: 481 LVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVDE 540
LVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFST SNSFKLNSVKKNSLNLVVVDE
Sbjct: 481 LVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTTSNSFKLNSVKKNSLNLVVVDE 540
Query: 541 AAQLKECESLIPLQLQHINHALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSKH 600
AAQLKECESLIPLQL HI+HA+LVGDEFQLPAT+KSK
Sbjct: 541 AAQLKECESLIPLQLPHISHAILVGDEFQLPATVKSK----------------------- 600
Query: 601 LLDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEE 660
IMDASIVMNK YEK YLPSPLFGPYSFINVCGG+EE
Sbjct: 601 ------------------------IMDASIVMNKEYEKEYLPSPLFGPYSFINVCGGEEE 660
Query: 661 SNGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEK 720
SNGDGQSKKN VEV VVTQI+QMLYKAWCKNK DISIG+ISPYNAQVSSIQEKLGRKYEK
Sbjct: 661 SNGDGQSKKNMVEVTVVTQIIQMLYKAWCKNKKDISIGIISPYNAQVSSIQEKLGRKYEK 720
Query: 721 NNNEGFRVKVKSIDGFQGGEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLWIV 780
NNEGF VKVKSIDGFQGGEEDVIIISTVRSN+GHNIGFLSNKQRTNVALTRARFCLWIV
Sbjct: 721 KNNEGFGVKVKSIDGFQGGEEDVIIISTVRSNNGHNIGFLSNKQRTNVALTRARFCLWIV 780
Query: 781 GDAKTLGKSNSEWRDVINDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLDNI 840
GDAKTLGKSNSEWRDVI+DAKTR+CFFNVEENKELANEMRM+KTWQ+ DIKQEILKLDNI
Sbjct: 781 GDAKTLGKSNSEWRDVIDDAKTRRCFFNVEENKELANEMRMMKTWQMSDIKQEILKLDNI 797
Query: 841 YNNNH-YGRV 848
YN+NH YGRV
Sbjct: 841 YNSNHVYGRV 797
BLAST of MELO3C020052.jh1 vs. NCBI nr
Match:
KAE8637320.1 (hypothetical protein CSA_019106 [Cucumis sativus])
HSP 1 Score: 1380 bits (3572), Expect = 0.0
Identity = 708/850 (83.29%), Postives = 743/850 (87.41%), Query Frame = 0
Query: 1 MEGEGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYK 60
ME EGGLS NK SNHK+SNGLIDALFSWDFNNVFNQN YK KVRKIPKSFETEEQYK
Sbjct: 1 MEAEGGLS----NKGSNHKDSNGLIDALFSWDFNNVFNQNIYKPKVRKIPKSFETEEQYK 60
Query: 61 ASYIFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILFDVNVSSWRNSDGGKG 120
SYIFPLLEETRAELCSNLKTIQKAPFSQVISIE++NTKK KILF+VNVSSWRN+ GGKG
Sbjct: 61 GSYIFPLLEETRAELCSNLKTIQKAPFSQVISIETSNTKKDKILFNVNVSSWRNTYGGKG 120
Query: 121 QQPYKSLPGDIFVILDADPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNM 180
QQPYKSLPGD FVILD DPQTITSDYLEKSSKLNWAFAWLGQ+NDNNTPTHL LHISN+M
Sbjct: 121 QQPYKSLPGDFFVILDVDPQTITSDYLEKSSKLNWAFAWLGQVNDNNTPTHLKLHISNSM 180
Query: 181 DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDNKTCKQCNGN-DGED 240
DQL STPLFIVFLMNLTTNLRIWKTLQCSS GGIVKH+LGT S+DNKTCKQCN DGED
Sbjct: 181 DQLKSTPLFIVFLMNLTTNLRIWKTLQCSSSGGIVKHVLGTMSMDNKTCKQCNNQTDGED 240
Query: 241 YSTQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWK 300
ST++ TLR
Sbjct: 241 -STEDMATLR-------------------------------------------------- 300
Query: 301 ILTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFD 360
TLACAPTNVAITNLASQV+ LLKHDSLS+N +FCPLGELLLFGNKDRLKFD
Sbjct: 301 ---------TLACAPTNVAITNLASQVLKLLKHDSLSRNAIFCPLGELLLFGNKDRLKFD 360
Query: 361 SHDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDCV 420
QL+DIYLDRRVEKLFKCLGQ+GLKFQI+SMIGIFQENKLSK+KRMFK N S LL+CV
Sbjct: 361 YSHQLEDIYLDRRVEKLFKCLGQYGLKFQISSMIGIFQENKLSKLKRMFKSNVSSLLECV 420
Query: 421 HIFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDDDDKMGEALIDLKCHCL 480
HIFTTHIP+QVIMEHNWKKLEILVGFICDIGTLLSK+NY+ DDDD MGEALIDLKCH L
Sbjct: 421 HIFTTHIPQQVIMEHNWKKLEILVGFICDIGTLLSKNNYNYDDDD-TMGEALIDLKCHFL 480
Query: 481 LVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVDE 540
LVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFST SNSFKLNSVKKNSLNLVVVDE
Sbjct: 481 LVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTTSNSFKLNSVKKNSLNLVVVDE 540
Query: 541 AAQLKECESLIPLQLQHINHALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSKH 600
AAQLKECESLIPLQL HI+HA+LVGDEFQLPAT+KSKVCE AKFGRSLYERLSL+GYSKH
Sbjct: 541 AAQLKECESLIPLQLPHISHAILVGDEFQLPATVKSKVCERAKFGRSLYERLSLIGYSKH 600
Query: 601 LLDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEE 660
LLDTQYRMHP VSYFPNSKFYGNKIMDASIVMNK YEK YLPSPLFGPYSFINVCGG+EE
Sbjct: 601 LLDTQYRMHPLVSYFPNSKFYGNKIMDASIVMNKEYEKEYLPSPLFGPYSFINVCGGEEE 660
Query: 661 SNGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEK 720
SNGDGQSKKN VEV VVTQI+QMLYKAWCKNK DISIG+ISPYNAQVSSIQEKLGRKYEK
Sbjct: 661 SNGDGQSKKNMVEVTVVTQIIQMLYKAWCKNKKDISIGIISPYNAQVSSIQEKLGRKYEK 720
Query: 721 NNNEGFRVKVKSIDGFQGGEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLWIV 780
NNEGF VKVKSIDGFQGGEEDVIIISTVRSN+GHNIGFLSNKQRTNVALTRARFCLWIV
Sbjct: 721 KNNEGFGVKVKSIDGFQGGEEDVIIISTVRSNNGHNIGFLSNKQRTNVALTRARFCLWIV 780
Query: 781 GDAKTLGKSNSEWRDVINDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLDNI 840
GDAKTLGKSNSEWRDVI+DAKTR+CFFNVEENKELANEMRM+KTWQ+ DIKQEILKLDNI
Sbjct: 781 GDAKTLGKSNSEWRDVIDDAKTRRCFFNVEENKELANEMRMMKTWQMSDIKQEILKLDNI 785
Query: 841 YNNNH-YGRV 848
YN+NH YGRV
Sbjct: 841 YNSNHVYGRV 785
BLAST of MELO3C020052.jh1 vs. NCBI nr
Match:
XP_038891794.1 (helicase SEN1-like [Benincasa hispida])
HSP 1 Score: 1380 bits (3572), Expect = 0.0
Identity = 704/846 (83.22%), Postives = 765/846 (90.43%), Query Frame = 0
Query: 4 EGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYKASY 63
EGG SS +NKASNHK+SNGLID LFSWDF NVFNQNFYKLKV KIPKSFE+EEQYK SY
Sbjct: 2 EGGGSSRSNNKASNHKDSNGLIDVLFSWDFKNVFNQNFYKLKVGKIPKSFESEEQYKGSY 61
Query: 64 IFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILFDVNVSSWRNSDGGKGQQP 123
+FPLLEETRAELC +LKTI KAPFSQV+SIE +N KKGK +F VNVS+W N++ KG QP
Sbjct: 62 LFPLLEETRAELCCSLKTIHKAPFSQVVSIEHSNKKKGKTMFSVNVSTWINTNK-KGHQP 121
Query: 124 YKSLPGDIFVILDADPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNMDQL 183
YK+LPGDIFVILD DPQTI+SDYLE SS+LNWAFAWLGQ NDNNTP++L LHISNNMDQ+
Sbjct: 122 YKALPGDIFVILDIDPQTISSDYLESSSELNWAFAWLGQANDNNTPSNLKLHISNNMDQV 181
Query: 184 N---STPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDNKTCKQCNGNDGEDY 243
+ ST LFIVFLMN+TTNLRIWK LQ S+DGGI+KHILGTTS N+TCKQCN N GED
Sbjct: 182 DVHKSTTLFIVFLMNVTTNLRIWKALQGSTDGGIIKHILGTTSTGNQTCKQCNQN-GED- 241
Query: 244 STQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWKI 303
STQNFPTL L SL NESQ+VAIESCIK VICQHKPSIELIWGPPGTGKTKTTSILLWKI
Sbjct: 242 STQNFPTLCLSSL--NESQKVAIESCIKNVICQHKPSIELIWGPPGTGKTKTTSILLWKI 301
Query: 304 LTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFDS 363
L + HQIRTLACAPTN+AITNLASQVV LLKHDSLSKND FCPLGELLLFGNKDRLKFDS
Sbjct: 302 LRMRHQIRTLACAPTNIAITNLASQVVKLLKHDSLSKNDFFCPLGELLLFGNKDRLKFDS 361
Query: 364 HDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDCVH 423
QL+DIYLD RVEKLFKCLGQ+GLKFQITSMIGI QENK SKMKRMFK S +L+CV+
Sbjct: 362 --QLEDIYLDHRVEKLFKCLGQNGLKFQITSMIGILQENKPSKMKRMFKSIGSSILECVN 421
Query: 424 IFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDDDDKMGEALIDLKCHCLL 483
I THIPKQVIMEHN +K+EILV I IGTLL K YDDD K+ E+L+DLKCHCLL
Sbjct: 422 ILVTHIPKQVIMEHNLEKMEILVELIGCIGTLLRK-GYDDD----KVRESLVDLKCHCLL 481
Query: 484 VLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVDEA 543
VLRTLLVSLDEIEVPSK+SKNSIEKFCFQ+ASLIF+TASNSFKLNSVKKNSLNL+VVDEA
Sbjct: 482 VLRTLLVSLDEIEVPSKVSKNSIEKFCFQQASLIFTTASNSFKLNSVKKNSLNLLVVDEA 541
Query: 544 AQLKECESLIPLQLQHINHALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSKHL 603
AQLKECESLIPLQLQHINHA+L+GDEFQLPATIKSKVCEGAKFGRSL+ERLSL+GYSKHL
Sbjct: 542 AQLKECESLIPLQLQHINHAILIGDEFQLPATIKSKVCEGAKFGRSLFERLSLLGYSKHL 601
Query: 604 LDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEES 663
L+TQYRMHP VS FPNSKFYGNKI+D SIVM+K YEK+YLPSPLFGPYSFINVCGGQEES
Sbjct: 602 LNTQYRMHPLVSCFPNSKFYGNKILDGSIVMDKEYEKHYLPSPLFGPYSFINVCGGQEES 661
Query: 664 NGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEKN 723
NGDGQSKKN VEV+VVTQI+QMLYKAWCKNK D+SIGVISPY AQVSSIQ+K GRKYEKN
Sbjct: 662 NGDGQSKKNMVEVVVVTQIIQMLYKAWCKNKKDLSIGVISPYAAQVSSIQDKFGRKYEKN 721
Query: 724 NNEGFRVKVKSIDGFQGGEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLWIVG 783
N EGFRVKVKSIDGFQGGEEDVIIISTVRSN+G +IGFLS+ QRTNVALTRARFCLWIVG
Sbjct: 722 N-EGFRVKVKSIDGFQGGEEDVIIISTVRSNNGKSIGFLSSTQRTNVALTRARFCLWIVG 781
Query: 784 DAKTLGKSNSEWRDVINDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLDNIY 843
DA TLGKSNSEWRDV+NDAK R+CFFNVE++KELA+ MRMIKTWQI DIK+EILKLDNIY
Sbjct: 782 DATTLGKSNSEWRDVVNDAKARKCFFNVEDDKELADGMRMIKTWQISDIKEEILKLDNIY 834
Query: 844 NNNHYG 846
NN+H G
Sbjct: 842 NNDHRG 834
BLAST of MELO3C020052.jh1 vs. NCBI nr
Match:
XP_022988087.1 (helicase sen1-like [Cucurbita maxima])
HSP 1 Score: 1206 bits (3119), Expect = 0.0
Identity = 614/847 (72.49%), Postives = 717/847 (84.65%), Query Frame = 0
Query: 4 EGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYKASY 63
EGG SCRS+KA++ K+SNGLID LFSW+ NVFNQN YKLKV IPKSFE+EE Y+ SY
Sbjct: 2 EGG-ESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY 61
Query: 64 IFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILFDVNVSSWRNSDGGKGQQP 123
+FPL EETRAELCS+LK I KAP +QV+SIE +NTK+GKILF+VNVS WR++DG KGQQP
Sbjct: 62 LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDG-KGQQP 121
Query: 124 YKSLPGDIFVILDADPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNM--- 183
YK+LPGDIF+ILD+DPQT SDYLE+S + NWAFAWLGQ+ DN+ PTHL LH+S N+
Sbjct: 122 YKALPGDIFIILDSDPQTTDSDYLERS-EFNWAFAWLGQITDNSIPTHLKLHVSKNITAQ 181
Query: 184 -DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDN-KTCKQCNGNDGE 243
D L ST FIVFLMN+TTNLRIWK LQCS+ GGI++ +LGTT + N ++C +C ND E
Sbjct: 182 GDILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKE 241
Query: 244 DYSTQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLW 303
D TQ++PT L SL NESQ+VAI++CI+ +CQHKPSI+LIWGPPGTGKTKTTSILLW
Sbjct: 242 D-PTQDYPTPHLSSL--NESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLW 301
Query: 304 KILTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKF 363
+ILT+ HQIRTLAC+PTNVAITNLASQVV LLK +S K+ +FCPLG+LLLFGNKDRLK
Sbjct: 302 RILTMKHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKV 361
Query: 364 DSHDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDC 423
DS QL++IY++ RVEKL KCLG +G KFQITSMI I Q +K ++KRMFK AS LL+C
Sbjct: 362 DS--QLEEIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLEC 421
Query: 424 VHIFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDDDDKMGEALIDLKCHC 483
VHI TTH+P++VIMEHN KK+EILV I DIGTLLS+D DDK+ LI LK C
Sbjct: 422 VHILTTHVPQEVIMEHNLKKMEILVELIGDIGTLLSED-------DDKVRGTLIGLKGQC 481
Query: 484 LLVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVD 543
+LVL+TLL+SLD++EVPSK+S+NSIEKFCFQ+ASLIFSTASNSFKL +VKKNSLNL+VVD
Sbjct: 482 VLVLQTLLMSLDQVEVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVD 541
Query: 544 EAAQLKECESLIPLQLQHINHALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSK 603
EAAQLKECESLIPLQL I HA+L+GDEFQLPAT+ SKV E A FG SL+ERLS++G+ K
Sbjct: 542 EAAQLKECESLIPLQLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFK 601
Query: 604 HLLDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKG-YEKNYLPSPLFGPYSFINVCGGQ 663
HLL+TQYRMHP VS+FPNSKFYGN+I+DASIVMNK YE++YLPSPLFGPYSFI+V GGQ
Sbjct: 602 HLLNTQYRMHPSVSHFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQ 661
Query: 664 EESNGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKY 723
EESN DGQSKKN EV+VV QI+QMLYKAW K DISIGVISPY AQVSSIQ KLGRKY
Sbjct: 662 EESNDDGQSKKNMFEVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKY 721
Query: 724 EKNNNEGFRVKVKSIDGFQGGEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLW 783
EK EGF +KVKS+DGFQGGEEDVIIISTVRSN G+NIGFLS+ QRTNVALTRAR CLW
Sbjct: 722 EK---EGFTIKVKSVDGFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLW 781
Query: 784 IVGDAKTLGKSNSEWRDVINDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLD 843
IVGDA TLGKSNSEWR+VI DAK+RQCFFNVEE++EL + M+M+KTWQ+ DI QEILKLD
Sbjct: 782 IVGDATTLGKSNSEWREVIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLD 830
BLAST of MELO3C020052.jh1 vs. ExPASy Swiss-Prot
Match:
Q00416 (Helicase SEN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEN1 PE=1 SV=2)
HSP 1 Score: 184.9 bits (468), Expect = 3.8e-45
Identity = 209/828 (25.24%), Postives = 361/828 (43.60%), Query Frame = 0
Query: 20 ESNGLIDALFSWDF--NNVFNQNFYKLKVRKIPKSFETEEQYKASYIFPLLEETRAELCS 79
+ N L + + WD+ N+ + + + F + Y+ LL E+ LCS
Sbjct: 1112 DMNPLYEIILQWDYTRNSEYPDDEPIGNYSDVKDFFNSPADYQKVMKPLLLLESWQGLCS 1171
Query: 80 NLKTIQKAPFSQVISIESTNTKKGKILFDVNVSSWRN--SDGGKGQQPYKSLPGDIFVIL 139
+ PFS ++ N +DV S + D G + D+ V+
Sbjct: 1172 SRDREDYKPFSIIVG----NRTAVSDFYDVYASVAKQVIQDCGISE-------SDLIVMA 1231
Query: 140 ---DADP-QTITSDYLEKSSKLNWA-FAWLGQLNDNNTPTHLNLHISNNMDQLNS--TPL 199
D P + ++SD +K+ A L N L +H +++ + + + +
Sbjct: 1232 YLPDFRPDKRLSSDDFKKAQHTCLAKVRTLKNTKGGNVDVTLRIHRNHSFSKFLTLRSEI 1291
Query: 200 FIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDNKTCKQCNGNDGEDYSTQNFPTL 259
+ V +M +TT R + TL+ +V IL Q + + T+
Sbjct: 1292 YCVKVMQMTTIEREYSTLEGLEYYDLVGQIL-----------QAKPSPPVNVDAAEIETV 1351
Query: 260 RLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWKILTINH--- 319
+ S LN SQ AI + + K LI GPPGTGKTKT ++ L+ +
Sbjct: 1352 K-KSYKLNTSQAEAIVNSVS------KEGFSLIQGPPGTGKTKTILGIIGYFLSTKNASS 1411
Query: 320 --------------------QIRTLACAPTNVAITNLASQVVNLLKHDSLSK-NDVFCPL 379
+ + L CAP+N A+ + + LK K F P
Sbjct: 1412 SNVIKVPLEKNSSNTEQLLKKQKILICAPSNAAVDEICLR----LKSGVYDKQGHQFKP- 1471
Query: 380 GELLLFGNKDRLKFDSHDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKM 439
+L+ G D + +KD+ L+ V+K +G+ + + + N ++K
Sbjct: 1472 -QLVRVGRSDVVNV----AIKDLTLEELVDKR---IGERNYEIRTDPELERKFNNAVTKR 1531
Query: 440 KRM-FKLNASFLLDCVHIFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDD 499
+ + KL++ + P+ + + KL++ + + I L +D
Sbjct: 1532 RELRGKLDSE----------SGNPESPMSTEDISKLQLKIRELSKIINELGRDR------ 1591
Query: 500 DDKMGEALIDLKCHCLLVLRTLLVSLDEIEVPSK-LSKNSIEKFCFQKASLIFSTASNS- 559
D+M E + + ++ L + + + + +I ST S S
Sbjct: 1592 -DEMREK-------------------NSVNYRNRDLDRRNAQAHILAVSDIICSTLSGSA 1651
Query: 560 FKLNSVKKNSLNLVVVDEAAQLKECESLIPLQLQHINHALLVGDEFQLPATIKSKVCEGA 619
+ + + V++DEA Q E S+IPL+ ++VGD QLP T+ S
Sbjct: 1652 HDVLATMGIKFDTVIIDEACQCTELSSIIPLRYGG-KRCIMVGDPNQLPPTVLSGAASNF 1711
Query: 620 KFGRSLYERLSLMGYSKHLLDTQYRMHPFVSYFPNSKFYGNKIMD--ASIVMNKGYEKNY 679
K+ +SL+ R+ S +LLD QYRMHP +S FP+S+FY ++ D ++NK
Sbjct: 1712 KYNQSLFVRME-KNSSPYLLDVQYRMHPSISKFPSSEFYQGRLKDGPGMDILNKRPWHQL 1771
Query: 680 LPSPLFGPYSFINVCGGQEESNGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDIS--IG 739
P PY F ++ G++E N S N E+ V ++V L++ + NK D + IG
Sbjct: 1772 EP---LAPYKFFDIISGRQEQNAKTMSYTNMEEIRVAIELVDYLFRKF-DNKIDFTGKIG 1831
Query: 740 VISPYNAQVSSIQEKLGRKYEKNNNEGFRVKVKSIDGFQGGEEDVIIISTVRS-NSGHNI 799
+ISPY Q+ ++++ R + N+ + +IDGFQG E+++I+IS VR+ ++ ++
Sbjct: 1832 IISPYREQMQKMRKEFARYFGGMINKS--IDFNTIDGFQGQEKEIILISCVRADDTKSSV 1852
Query: 800 GFLSNKQRTNVALTRARFCLWIVGDAKTLGKSNSEWRDVINDAKTRQC 805
GFL + +R NVALTRA+ +W++G ++L KS WRD+I DAK R C
Sbjct: 1892 GFLKDFRRMNVALTRAKTSIWVLGHQRSLAKSKL-WRDLIEDAKDRSC 1852
BLAST of MELO3C020052.jh1 vs. ExPASy Swiss-Prot
Match:
B6SFA4 (Probable helicase MAGATAMA 3 OS=Arabidopsis thaliana OX=3702 GN=MAA3 PE=2 SV=1)
HSP 1 Score: 181.4 bits (459), Expect = 4.2e-44
Identity = 117/336 (34.82%), Postives = 184/336 (54.76%), Query Frame = 0
Query: 501 NSIEKFCFQKASLIFSTASNSFKLNSVKKN-SLNLVVVDEAAQLKECESLIPLQLQHINH 560
+SI ++A+++F+T S S K N ++V++DEAAQ E +LIPL +
Sbjct: 452 DSIRTAILEEAAIVFATLSFSGSALLAKSNRGFDVVIIDEAAQAVEPATLIPLATR-CKQ 511
Query: 561 ALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSKHLLDTQYRMHPFVSYFPNSKF 620
LVGD QLPAT+ S V + + +G S++ERL GY +L TQYRMHP + FP+ +F
Sbjct: 512 VFLVGDPKQLPATVISTVAQDSGYGTSMFERLQKAGYPVKMLKTQYRMHPEIRSFPSKQF 571
Query: 621 YGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEESN-GDGQSKKNTVEVIVVTQ 680
Y + D S + +++ FGP+ F ++ G+E + G S+ N EV V
Sbjct: 572 YEGALEDGSDI-EAQTTRDWHKYRCFGPFCFFDIHEGKESQHPGATGSRVNLDEVEFVLL 631
Query: 681 IVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEKNNNEGFRVKVKSIDGFQGG 740
I L + + K+ + +ISPYN QV + +++ + + V + ++DGFQG
Sbjct: 632 IYHRLVTMYPELKSSSQLAIISPYNYQVKTFKDRFKEMFGTEAEK--VVDINTVDGFQGR 691
Query: 741 EEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLWIVGDAKTLGKSNSEWRDVIND 800
E+DV I S VR+N IGFLSN +R NV +TRA+ + +VG A TL KS+ W+++I
Sbjct: 692 EKDVAIFSCVRANENGQIGFLSNSRRMNVGITRAKSSVLVVGSAATL-KSDPLWKNLIES 751
Query: 801 AKTRQCFFNVEE------NKELANEMRMIKTWQICD 829
A+ R F V + ++E M++ + +I D
Sbjct: 752 AEQRNRLFKVSKPLNNFFSEENLETMKLTEDMEIPD 782
BLAST of MELO3C020052.jh1 vs. ExPASy Swiss-Prot
Match:
Q92355 (Helicase sen1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sen1 PE=1 SV=1)
HSP 1 Score: 159.1 bits (401), Expect = 2.2e-37
Identity = 113/328 (34.45%), Postives = 182/328 (55.49%), Query Frame = 0
Query: 498 LSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNL--VVVDEAAQLKECESLIPLQLQ 557
L + +K ++A ++ +T S S + V +SLN V++DEAAQ E +++IPL+
Sbjct: 1337 LLRKKAQKAILKQADVVCATLSGSGH-DLVAHSSLNFSTVIIDEAAQAVELDTIIPLR-Y 1396
Query: 558 HINHALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSKH--LLDTQYRMHPFVSY 617
+LVGD QLP T+ SK + +SL+ R+ +S LL QYRMHP +S+
Sbjct: 1397 GAKKCILVGDPNQLPPTVLSKKAASLNYSQSLFVRIQ-KNFSNQMCLLSIQYRMHPDISH 1456
Query: 618 FPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEESNGDGQSKKNTVEV 677
FP+ KFY +++ D + K + ++ +P F Y +V G + SN S N EV
Sbjct: 1457 FPSKKFYDSRLEDGDNMAEKTQQVWHV-NPKFTQYRLFDVRGKERTSN--TMSTYNLEEV 1516
Query: 678 IVVTQIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEKNNNEGFRVKVKSID 737
+ +V L + IGVI+PY +Q+ ++ KY K+ + ++++D
Sbjct: 1517 EYLVNMVDELLNKFPDVNFTGRIGVITPYRSQLHELRRAFKVKYGKSFMS--TIDIQTVD 1576
Query: 738 GFQGGEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLWIVGDAKTLGKSNSEWR 797
GFQG E+D+I S V+S S H IGFL + +R NVALTRAR L I+G+ +TL K++ W
Sbjct: 1577 GFQGQEKDIIFFSCVKSYSKHGIGFLRDFRRLNVALTRARSSLLIIGNMETL-KTDDLWG 1636
Query: 798 DVINDAKTRQCFFNVEENKELANEMRMI 822
+++DA +R+ + E+ + +E R+I
Sbjct: 1637 SLVDDALSRK----LVESPHIDSEGRLI 1651
BLAST of MELO3C020052.jh1 vs. ExPASy Swiss-Prot
Match:
O94387 (Uncharacterized ATP-dependent helicase C29A10.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC29A10.10c PE=3 SV=1)
HSP 1 Score: 157.1 bits (396), Expect = 8.5e-37
Identity = 122/364 (33.52%), Postives = 184/364 (50.55%), Query Frame = 0
Query: 500 KNSIEKFCFQKASLIFSTASNS-FKLNSVKKNSLNLVVVDEAAQLKECESLIPLQLQHIN 559
K I+ Q+A ++ +T S S +L + V++DEAAQ E S+IPL+
Sbjct: 1477 KKQIQNQLLQEADIVCATLSASGHELLLNAGLTFRTVIIDEAAQAVELSSIIPLK-YGCE 1536
Query: 560 HALLVGDEFQLPATIKSKVCEGAKFGRSLYERL-SLMGYSKHLLDTQYRMHPFVSYFPNS 619
++VGD QLP T+ SK + +SLY R+ S LL QYRM+P +S FP+
Sbjct: 1537 SCVMVGDPNQLPPTVLSKTSAKFGYSQSLYVRMFKQHNESACLLSIQYRMNPEISRFPSK 1596
Query: 620 KFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEESNGDGQSKKNTVEVIVVT 679
FY +K++D M+ + + P G Y F NV G + SN +S N E +
Sbjct: 1597 FFYNSKLLDGP-NMSAVTSRPWHEDPQLGIYRFFNVHGTEAFSN--SKSLYNVEEASFIL 1656
Query: 680 QIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEKNNNEGFRVKVKSIDGFQG 739
+ + L + + + IGV++PY +QV ++ + RKY + + + ++DGFQG
Sbjct: 1657 LLYERLIQCYLNIDFEGKIGVVTPYRSQVQQLRSQFQRKYGSIIFK--HLDIHTVDGFQG 1716
Query: 740 GEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLWIVGDAKTL------------ 799
E+D+II S VRS+ IGFL + +R NVALTRA+ L+IVG++K L
Sbjct: 1717 QEKDIIIFSCVRSSMSGGIGFLQDLRRLNVALTRAKSSLYIVGNSKPLMQEDIFYSLIED 1776
Query: 800 GKSNSEWRDV-INDAKTRQCFFNV-----EENKELANEMRMIKTWQ--ICDIKQEILKLD 842
K+ WRD+ N K + NV N LA+ IK+ IC+ KQE K+
Sbjct: 1777 AKTRGVWRDLSANQFKNSKSISNVSTHLASNNLNLASRDTPIKSPSVGICEEKQEAHKVK 1834
BLAST of MELO3C020052.jh1 vs. ExPASy Swiss-Prot
Match:
Q86AS0 (Probable helicase DDB_G0274399 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274399 PE=3 SV=1)
HSP 1 Score: 155.2 bits (391), Expect = 3.2e-36
Identity = 108/317 (34.07%), Postives = 166/317 (52.37%), Query Frame = 0
Query: 501 NSIEKFCFQKASLIFSTAS-NSFKLNSVKKNSLNLVVVDEAAQLKECESLIPLQLQHINH 560
+SI +A ++ +T S + L + ++V++DEAAQ E +LIP+Q
Sbjct: 553 SSIRTLVLDEADIVATTLSFSGASLLTKMAGGFDIVIIDEAAQAVETSTLIPIQ-HGCKK 612
Query: 561 ALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSKHLLDTQYRMHPFVSYFPNSKF 620
+LVGD QLPATI S + K+ +SL++RL S H+L TQYRMH + FP+ F
Sbjct: 613 VVLVGDPKQLPATIISPLAIKYKYDQSLFQRLQEKN-SPHMLTTQYRMHSLIRAFPSRHF 672
Query: 621 YGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEESNGDGQSKKNTVEVIVVTQI 680
Y + ++D + ++ +Y +P FGP F ++ E G G S N E + +
Sbjct: 673 YQDLLLDGPNIPSRA--THYHSNPFFGPLVFYDLSWSTETKPGGG-SVFNEHECKMAMYL 732
Query: 681 VQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEKNNNEGFRVKVKSIDGFQGGE 740
Q+ K + IG+ISPY QV +++E N G + + ++DGFQG E
Sbjct: 733 FQLFTKVYPDEDFASRIGIISPYRQQVLALREIF------KNYPG--ISIDTVDGFQGRE 792
Query: 741 EDVIIISTVRS--NSGHNIGFLSNKQRTNVALTRARFCLWIVGDAKTLGKSNSEWRDVIN 800
++II S VR+ G IGFLS+ +R NVALTR R L I+G+ K L N +W ++I
Sbjct: 793 REIIIFSCVRAPVEEGAGIGFLSDVRRMNVALTRPRSSLLILGNTKAL-SINKDWNELIQ 852
Query: 801 DAKTRQCFFNVEENKEL 815
+ Q V +++ L
Sbjct: 853 HTQNNQQLIPVTKDQPL 855
BLAST of MELO3C020052.jh1 vs. ExPASy TrEMBL
Match:
A0A1S3C4A0 (helicase SEN1-like OS=Cucumis melo OX=3656 GN=LOC103496680 PE=4 SV=1)
HSP 1 Score: 1691 bits (4378), Expect = 0.0
Identity = 837/848 (98.70%), Postives = 843/848 (99.41%), Query Frame = 0
Query: 1 MEGEGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYK 60
MEGEGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYK
Sbjct: 1 MEGEGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYK 60
Query: 61 ASYIFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILFDVNVSSWRNSDGGKG 120
ASYIFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILF+VNVSSWRN DGGKG
Sbjct: 61 ASYIFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILFNVNVSSWRNRDGGKG 120
Query: 121 QQPYKSLPGDIFVILDADPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNM 180
QQPYKSLPGDIFVILD DPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNM
Sbjct: 121 QQPYKSLPGDIFVILDTDPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNM 180
Query: 181 DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDNKTCKQCNGNDGEDY 240
DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDNKTCKQCNGNDGEDY
Sbjct: 181 DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDNKTCKQCNGNDGEDY 240
Query: 241 STQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWKI 300
STQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWKI
Sbjct: 241 STQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWKI 300
Query: 301 LTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFDS 360
LTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFDS
Sbjct: 301 LTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFDS 360
Query: 361 HDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDCVH 420
HDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDCVH
Sbjct: 361 HDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDCVH 420
Query: 421 IFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDDDDKMGEALIDLKCHCLL 480
IFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDD KMGEALIDLKCHCLL
Sbjct: 421 IFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDD--KMGEALIDLKCHCLL 480
Query: 481 VLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVDEA 540
VLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVDEA
Sbjct: 481 VLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVDEA 540
Query: 541 AQLKECESLIPLQLQHINHALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSKHL 600
AQLKECESL+PLQLQHI HALLVGDEFQLPATIKSK+CEGAKFGRSLYERLSLMGYSKHL
Sbjct: 541 AQLKECESLVPLQLQHITHALLVGDEFQLPATIKSKICEGAKFGRSLYERLSLMGYSKHL 600
Query: 601 LDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEES 660
LDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEES
Sbjct: 601 LDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEES 660
Query: 661 NGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEKN 720
NGDGQSKKNTVEVIVVTQI+QMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEKN
Sbjct: 661 NGDGQSKKNTVEVIVVTQIIQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEKN 720
Query: 721 NNEGFRVKVKSIDGFQGGEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLWIVG 780
NNEGFRVKVKSIDGFQGGEEDVIIISTVRSN+GHNIGFLSNKQRTNVALTRARFCLWIVG
Sbjct: 721 NNEGFRVKVKSIDGFQGGEEDVIIISTVRSNNGHNIGFLSNKQRTNVALTRARFCLWIVG 780
Query: 781 DAKTLGKSNSEWRDVINDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLDNIY 840
DAKTLGKSNSEWRD+INDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLDNIY
Sbjct: 781 DAKTLGKSNSEWRDIINDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLDNIY 840
Query: 841 NNNHYGRV 848
NNNHYGRV
Sbjct: 841 NNNHYGRV 846
BLAST of MELO3C020052.jh1 vs. ExPASy TrEMBL
Match:
A0A0A0KMQ3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G292250 PE=4 SV=1)
HSP 1 Score: 1518 bits (3930), Expect = 0.0
Identity = 763/850 (89.76%), Postives = 799/850 (94.00%), Query Frame = 0
Query: 1 MEGEGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYK 60
ME EGGLS NK SNHK+SNGLIDALFSWDFNNVFNQN YK KVRKIPKSFETEEQYK
Sbjct: 1 MEAEGGLS----NKGSNHKDSNGLIDALFSWDFNNVFNQNIYKPKVRKIPKSFETEEQYK 60
Query: 61 ASYIFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILFDVNVSSWRNSDGGKG 120
SYIFPLLEETRAELCSNLKTIQKAPFSQVISIE++NTKK KILF+VNVSSWRN+ GGKG
Sbjct: 61 GSYIFPLLEETRAELCSNLKTIQKAPFSQVISIETSNTKKDKILFNVNVSSWRNTYGGKG 120
Query: 121 QQPYKSLPGDIFVILDADPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNM 180
QQPYKSLPGD FVILD DPQTITSDYLEKSSKLNWAFAWLGQ+NDNNTPTHL LHISN+M
Sbjct: 121 QQPYKSLPGDFFVILDVDPQTITSDYLEKSSKLNWAFAWLGQVNDNNTPTHLKLHISNSM 180
Query: 181 DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDNKTCKQCNGN-DGED 240
DQL STPLFIVFLMNLTTNLRIWKTLQCSS GGIVKH+LGT S+DNKTCKQCN DGED
Sbjct: 181 DQLKSTPLFIVFLMNLTTNLRIWKTLQCSSSGGIVKHVLGTMSMDNKTCKQCNNQTDGED 240
Query: 241 YSTQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWK 300
ST++ TLRL SLSLNESQRVAIESCI+KV CQHKPSIELIWGPPGTGKTKTTSILLWK
Sbjct: 241 -STEDMATLRLASLSLNESQRVAIESCIRKVKCQHKPSIELIWGPPGTGKTKTTSILLWK 300
Query: 301 ILTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFD 360
IL INHQIRTLACAPTNVAITNLASQV+ LLKHDSLS+N +FCPLGELLLFGNKDRLKFD
Sbjct: 301 ILAINHQIRTLACAPTNVAITNLASQVLKLLKHDSLSRNAIFCPLGELLLFGNKDRLKFD 360
Query: 361 SHDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDCV 420
QL+DIYLDRRVEKLFKCLGQ+GLKFQI+SMIGIFQENKLSK+KRMFK N S LL+CV
Sbjct: 361 YSHQLEDIYLDRRVEKLFKCLGQYGLKFQISSMIGIFQENKLSKLKRMFKSNVSSLLECV 420
Query: 421 HIFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDDDDKMGEALIDLKCHCL 480
HIFTTHIP+QVIMEHNWKKLEILVGFICDIGTLLSK+NY+ DDDD MGEALIDLKCH L
Sbjct: 421 HIFTTHIPQQVIMEHNWKKLEILVGFICDIGTLLSKNNYNYDDDD-TMGEALIDLKCHFL 480
Query: 481 LVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVDE 540
LVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFST SNSFKLNSVKKNSLNLVVVDE
Sbjct: 481 LVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTTSNSFKLNSVKKNSLNLVVVDE 540
Query: 541 AAQLKECESLIPLQLQHINHALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSKH 600
AAQLKECESLIPLQL HI+HA+LVGDEFQLPAT+KSKVCE AKFGRSLYERLSL+GYSKH
Sbjct: 541 AAQLKECESLIPLQLPHISHAILVGDEFQLPATVKSKVCERAKFGRSLYERLSLIGYSKH 600
Query: 601 LLDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEE 660
LLDTQYRMHP VSYFPNSKFYGNKIMDASIVMNK YEK YLPSPLFGPYSFINVCGG+EE
Sbjct: 601 LLDTQYRMHPLVSYFPNSKFYGNKIMDASIVMNKEYEKEYLPSPLFGPYSFINVCGGEEE 660
Query: 661 SNGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEK 720
SNGDGQSKKN VEV VVTQI+QMLYKAWCKNK DISIG+ISPYNAQVSSIQEKLGRKYEK
Sbjct: 661 SNGDGQSKKNMVEVTVVTQIIQMLYKAWCKNKKDISIGIISPYNAQVSSIQEKLGRKYEK 720
Query: 721 NNNEGFRVKVKSIDGFQGGEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLWIV 780
NNEGF VKVKSIDGFQGGEEDVIIISTVRSN+GHNIGFLSNKQRTNVALTRARFCLWIV
Sbjct: 721 KNNEGFGVKVKSIDGFQGGEEDVIIISTVRSNNGHNIGFLSNKQRTNVALTRARFCLWIV 780
Query: 781 GDAKTLGKSNSEWRDVINDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLDNI 840
GDAKTLGKSNSEWRDVI+DAKTR+CFFNVEENKELANEMRM+KTWQ+ DIKQEILKLDNI
Sbjct: 781 GDAKTLGKSNSEWRDVIDDAKTRRCFFNVEENKELANEMRMMKTWQMSDIKQEILKLDNI 840
Query: 841 YNNNH-YGRV 848
YN+NH YGRV
Sbjct: 841 YNSNHVYGRV 844
BLAST of MELO3C020052.jh1 vs. ExPASy TrEMBL
Match:
A0A6J1JC41 (helicase sen1-like OS=Cucurbita maxima OX=3661 GN=LOC111485435 PE=4 SV=1)
HSP 1 Score: 1206 bits (3119), Expect = 0.0
Identity = 614/847 (72.49%), Postives = 717/847 (84.65%), Query Frame = 0
Query: 4 EGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYKASY 63
EGG SCRS+KA++ K+SNGLID LFSW+ NVFNQN YKLKV IPKSFE+EE Y+ SY
Sbjct: 2 EGG-ESCRSHKANSAKDSNGLIDVLFSWELRNVFNQNLYKLKVGNIPKSFESEEHYRGSY 61
Query: 64 IFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILFDVNVSSWRNSDGGKGQQP 123
+FPL EETRAELCS+LK I KAP +QV+SIE +NTK+GKILF+VNVS WR++DG KGQQP
Sbjct: 62 LFPLFEETRAELCSSLKAIHKAPSAQVVSIEESNTKRGKILFNVNVSPWRSTDG-KGQQP 121
Query: 124 YKSLPGDIFVILDADPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNM--- 183
YK+LPGDIF+ILD+DPQT SDYLE+S + NWAFAWLGQ+ DN+ PTHL LH+S N+
Sbjct: 122 YKALPGDIFIILDSDPQTTDSDYLERS-EFNWAFAWLGQITDNSIPTHLKLHVSKNITAQ 181
Query: 184 -DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDN-KTCKQCNGNDGE 243
D L ST FIVFLMN+TTNLRIWK LQCS+ GGI++ +LGTT + N ++C +C ND E
Sbjct: 182 GDILKSTTFFIVFLMNVTTNLRIWKALQCSAGGGIIERVLGTTWLGNHQSCTECTQNDKE 241
Query: 244 DYSTQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLW 303
D TQ++PT L SL NESQ+VAI++CI+ +CQHKPSI+LIWGPPGTGKTKTTSILLW
Sbjct: 242 D-PTQDYPTPHLSSL--NESQKVAIQTCIENTLCQHKPSIDLIWGPPGTGKTKTTSILLW 301
Query: 304 KILTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKF 363
+ILT+ HQIRTLAC+PTNVAITNLASQVV LLK +S K+ +FCPLG+LLLFGNKDRLK
Sbjct: 302 RILTMKHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKV 361
Query: 364 DSHDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDC 423
DS QL++IY++ RVEKL KCLG +G KFQITSMI I Q +K ++KRMFK AS LL+C
Sbjct: 362 DS--QLEEIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGSKFPRLKRMFKSIASSLLEC 421
Query: 424 VHIFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDDDDKMGEALIDLKCHC 483
VHI TTH+P++VIMEHN KK+EILV I DIGTLLS+D DDK+ LI LK C
Sbjct: 422 VHILTTHVPQEVIMEHNLKKMEILVELIGDIGTLLSED-------DDKVRGTLIGLKGQC 481
Query: 484 LLVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVD 543
+LVL+TLL+SLD++EVPSK+S+NSIEKFCFQ+ASLIFSTASNSFKL +VKKNSLNL+VVD
Sbjct: 482 VLVLQTLLMSLDQVEVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVD 541
Query: 544 EAAQLKECESLIPLQLQHINHALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSK 603
EAAQLKECESLIPLQL I HA+L+GDEFQLPAT+ SKV E A FG SL+ERLS++G+ K
Sbjct: 542 EAAQLKECESLIPLQLPDIRHAILIGDEFQLPATVSSKVSEAAGFGTSLFERLSVLGHFK 601
Query: 604 HLLDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKG-YEKNYLPSPLFGPYSFINVCGGQ 663
HLL+TQYRMHP VS+FPNSKFYGN+I+DASIVMNK YE++YLPSPLFGPYSFI+V GGQ
Sbjct: 602 HLLNTQYRMHPSVSHFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFIDVSGGQ 661
Query: 664 EESNGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKY 723
EESN DGQSKKN EV+VV QI+QMLYKAW K DISIGVISPY AQVSSIQ KLGRKY
Sbjct: 662 EESNDDGQSKKNMFEVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKY 721
Query: 724 EKNNNEGFRVKVKSIDGFQGGEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLW 783
EK EGF +KVKS+DGFQGGEEDVIIISTVRSN G+NIGFLS+ QRTNVALTRAR CLW
Sbjct: 722 EK---EGFTIKVKSVDGFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLW 781
Query: 784 IVGDAKTLGKSNSEWRDVINDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLD 843
IVGDA TLGKSNSEWR+VI DAK+RQCFFNVEE++EL + M+M+KTWQ+ DI QEILKLD
Sbjct: 782 IVGDATTLGKSNSEWREVIKDAKSRQCFFNVEEDEELGDAMKMMKTWQMSDINQEILKLD 830
BLAST of MELO3C020052.jh1 vs. ExPASy TrEMBL
Match:
A0A6J1FQ96 (helicase SEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111447261 PE=4 SV=1)
HSP 1 Score: 1196 bits (3093), Expect = 0.0
Identity = 616/847 (72.73%), Postives = 713/847 (84.18%), Query Frame = 0
Query: 4 EGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYKASY 63
EGG SCR +K S+ K+SNGLID LFSW+ NVFNQN YKLKV IPKSFE+EE Y+ SY
Sbjct: 2 EGG-ESCRRHKPSSAKDSNGLIDVLFSWELTNVFNQNLYKLKVGNIPKSFESEEHYRGSY 61
Query: 64 IFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKGKILFDVNVSSWRNSDGGKGQQP 123
+FPLLEETRAELCS+LK I KAP +QV+SIE +N K+GKILF+VNVS WR++DG KGQQP
Sbjct: 62 LFPLLEETRAELCSSLKAIHKAPSAQVVSIEESNAKRGKILFNVNVSPWRSTDG-KGQQP 121
Query: 124 YKSLPGDIFVILDADPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNM--- 183
YK+LPG IF+ILD+DPQT SDYLE+S + NWAFAWLGQ+ DN+ PTHLNLH+S N+
Sbjct: 122 YKALPGHIFIILDSDPQTTDSDYLERS-EFNWAFAWLGQITDNSIPTHLNLHVSKNITAQ 181
Query: 184 -DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDN-KTCKQCNGNDGE 243
D L ST LFIVFLMN+TTNLRIWK LQCS+ GGI+ +LGTT + N ++C +C ND E
Sbjct: 182 GDILESTTLFIVFLMNVTTNLRIWKALQCSAGGGIIGRVLGTTWLGNHQSCTECTQNDQE 241
Query: 244 DYSTQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLW 303
D TQ++PT PS SLNESQ+VAIE+CI+ +CQHKPSI+LIWGPPGTGKTKTTSILLW
Sbjct: 242 D-PTQDYPTPH-PS-SLNESQKVAIETCIQNTLCQHKPSIDLIWGPPGTGKTKTTSILLW 301
Query: 304 KILTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKF 363
+ILT+ HQIRTLAC+PTNVAITNLASQVV LLK +S K+ +FCPLG+LLLFGNKDRLK
Sbjct: 302 RILTMKHQIRTLACSPTNVAITNLASQVVKLLKDESFRKDHIFCPLGQLLLFGNKDRLKV 361
Query: 364 DSHDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLLDC 423
DS QL++IY++ RVEKL KCLG +G KFQITSMI I Q K ++KRMFK AS LL+C
Sbjct: 362 DS--QLEEIYVEHRVEKLIKCLGPNGWKFQITSMIEILQGCKFPRLKRMFKSIASSLLEC 421
Query: 424 VHIFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDDDDKMGEALIDLKCHC 483
VHI TTH+P++VIMEHN KK+EILV I DIGTLLS+D DDK+ LI LK C
Sbjct: 422 VHILTTHVPQEVIMEHNLKKMEILVELIGDIGTLLSED-------DDKVRGTLIGLKGQC 481
Query: 484 LLVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVD 543
+LVL+TLL+SLD++EVPSK+S+NSIEKFCFQ+ASLIFSTASNSFKL +VKKNSLNL+VVD
Sbjct: 482 VLVLQTLLMSLDQVEVPSKVSRNSIEKFCFQQASLIFSTASNSFKLKNVKKNSLNLLVVD 541
Query: 544 EAAQLKECESLIPLQLQHINHALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSK 603
EAAQLKECESLIPLQL I HA+L+GDEFQLPATI SKV E A FG SL+ERLS++G+ K
Sbjct: 542 EAAQLKECESLIPLQLPDIRHAILIGDEFQLPATISSKVSEAAGFGTSLFERLSVLGHFK 601
Query: 604 HLLDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKG-YEKNYLPSPLFGPYSFINVCGGQ 663
HLL+TQYRMHP VS+FPNSKFYGN+I+DASIVMNK YE++YLPSPLFGPYSFINV GGQ
Sbjct: 602 HLLNTQYRMHPSVSHFPNSKFYGNQILDASIVMNKQLYEEHYLPSPLFGPYSFINVSGGQ 661
Query: 664 EESNGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKY 723
EESN DGQSKKN EV+VV QI+QMLYKAW K DISIGVISPY AQVSSIQ KLGRKY
Sbjct: 662 EESNDDGQSKKNMFEVVVVAQIIQMLYKAWSNKKKDISIGVISPYAAQVSSIQHKLGRKY 721
Query: 724 EKNNNEGFRVKVKSIDGFQGGEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLW 783
EK EGF +KVKS+DGFQGGEEDVIIISTVRSN G+NIGFLS+ QRTNVALTRAR CLW
Sbjct: 722 EKK--EGFTIKVKSVDGFQGGEEDVIIISTVRSNRGNNIGFLSSSQRTNVALTRARHCLW 781
Query: 784 IVGDAKTLGKSNSEWRDVINDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILKLD 843
IVGDA TLGKSNSEWR+VI DAK+RQC FNVEE+KEL + M+M+KT Q+ DI QEIL LD
Sbjct: 782 IVGDATTLGKSNSEWREVIKDAKSRQCLFNVEEDKELGDAMKMMKTSQMSDINQEILNLD 831
BLAST of MELO3C020052.jh1 vs. ExPASy TrEMBL
Match:
A0A6J1DR27 (helicase SEN1-like OS=Momordica charantia OX=3673 GN=LOC111023039 PE=4 SV=1)
HSP 1 Score: 1098 bits (2840), Expect = 0.0
Identity = 577/849 (67.96%), Postives = 692/849 (81.51%), Query Frame = 0
Query: 4 EGGLSSCRSNKASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYKASY 63
EGG SCRS KA N K+ NGLID LFSW+ +VFNQNFYKLKV KIPKSFE+E Y++SY
Sbjct: 2 EGG-ESCRSKKA-NAKDCNGLIDILFSWELRHVFNQNFYKLKVGKIPKSFESEHHYRSSY 61
Query: 64 IFPLLEETRAELCSNLKTIQKAPFSQVISIESTNTKKG-KILFDVNVSSWRNSDGGKGQQ 123
I+PLLEETRA+LCS+LKTI KAP +Q++SIE KG KILF+VN+SSWR ++G QQ
Sbjct: 62 IYPLLEETRADLCSSLKTIHKAPSAQLLSIEKEPKPKGPKILFNVNLSSWRINNGKGQQQ 121
Query: 124 PYKSLPGDIFVILDADPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPT-HLNLHISNNM- 183
Y+ LPGDIFVILD DPQT T LE S+ WAFAWLG + DNN PT HL L++S ++
Sbjct: 122 SYRPLPGDIFVILDIDPQTTTD--LECSN--TWAFAWLGHIIDNNAPTTHLKLYVSKDIS 181
Query: 184 ---DQLNSTPLFIVFLMNLTTNLRIWKTLQCSSDGGIVKHILGTTSIDNKTCKQCNGNDG 243
D +T LFIVFLMN+TTNLRIWK LQ S+D GIVK +LG+T N+TCK+C+
Sbjct: 182 AEGDIHQTTTLFIVFLMNVTTNLRIWKALQSSADVGIVKRVLGSTG--NQTCKECS---- 241
Query: 244 EDYSTQNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILL 303
E+ S +N PT S SLNESQ++AIESC+ V+CQHKPSI+LIWGPPGTGKTKTTS+LL
Sbjct: 242 EENSIEN-PTTLSSSSSLNESQKLAIESCVHNVLCQHKPSIDLIWGPPGTGKTKTTSMLL 301
Query: 304 WKILTINHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKN-DVFCPLGELLLFGNKDRL 363
KIL + HQIRTLACAPTN+AITNLAS+VV LLK ++ SK ++ PLGELLLFGNKDRL
Sbjct: 302 LKILRMTHQIRTLACAPTNIAITNLASRVVKLLKEEAFSKEYKLYSPLGELLLFGNKDRL 361
Query: 364 KFDSHDQLKDIYLDRRVEKLFKCLGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFLL 423
K DS +L+++YL+ RVE L KCLG++G KFQITSMI +E K + FK A L
Sbjct: 362 KIDS--ELEEVYLENRVESLVKCLGKNGWKFQITSMIEFLEEGK--RRIHHFKSIAPALH 421
Query: 424 DCVHIFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYDDDDDDDKMGEALIDLKC 483
+C++ TH+P QVI+EHN KK+EILV + D GTLL ++ D + E L DLK
Sbjct: 422 ECLYSLATHVPPQVILEHNLKKMEILVKLVDDFGTLLPQN-----DSKVSVRENLADLKR 481
Query: 484 HCLLVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVV 543
CLL L+ LLVSL++IEVPSK+S+NSIEKFCFQKASLIFSTASNSFKLNSVKKNS++L+V
Sbjct: 482 KCLLGLKNLLVSLEQIEVPSKVSRNSIEKFCFQKASLIFSTASNSFKLNSVKKNSVDLLV 541
Query: 544 VDEAAQLKECESLIPLQLQHINHALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGY 603
+DEAAQLKECESLIPLQ+ +I HA+L+GDEFQLPA + SKVC+ A +GRSL+ERLSL+GY
Sbjct: 542 IDEAAQLKECESLIPLQVPNIRHAILIGDEFQLPAIVSSKVCDAAGYGRSLFERLSLLGY 601
Query: 604 SKHLLDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGG 663
S HLLDTQYRMHP VS+FPNSKFYGN+I+DASIVM+K YE++YL P+FGPYSFI+VCGG
Sbjct: 602 STHLLDTQYRMHPCVSFFPNSKFYGNQILDASIVMDKQYERSYLAGPMFGPYSFIHVCGG 661
Query: 664 QEESNGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRK 723
QEESN DGQSKKN VEV+VVTQI+QMLYKAWCK+K D+SIGVISPY AQVS+IQ K+G K
Sbjct: 662 QEESNRDGQSKKNMVEVVVVTQIIQMLYKAWCKSKRDVSIGVISPYAAQVSAIQNKVGGK 721
Query: 724 YEKNN-NEGFRVKVKSIDGFQGGEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFC 783
YEKN NEGF VKVKS+DGFQGGEEDVIIISTVRSN ++IGFLS+ QRTNVALTRAR+C
Sbjct: 722 YEKNIINEGFTVKVKSVDGFQGGEEDVIIISTVRSNDRNDIGFLSSSQRTNVALTRARYC 781
Query: 784 LWIVGDAKTLGKSNSEWRDVINDAKTRQCFFNVEENKELANEMRMIKTWQICDIKQEILK 843
LWIVGDA+TL KSNSEW+DVI+DAK+R CFFNV+E+KELA+ MRM KTWQ+ +IK+E+LK
Sbjct: 782 LWIVGDAETLEKSNSEWKDVIDDAKSRHCFFNVDEDKELADAMRMTKTWQMSNIKEELLK 828
BLAST of MELO3C020052.jh1 vs. TAIR 10
Match:
AT1G65810.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )
HSP 1 Score: 533.1 bits (1372), Expect = 4.1e-151
Identity = 334/840 (39.76%), Postives = 482/840 (57.38%), Query Frame = 0
Query: 24 LIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYKASYIFPLLEETRAELCSNLKTIQ 83
L+D +FSW +V N N Y+ +V KIP +F + ++Y S++ P++EET A+L S++ TI+
Sbjct: 22 LVDVVFSWSLRDVLNSNLYRGQVGKIPNTFTSTKEYFESFVKPIIEETHADLLSSMGTIR 81
Query: 84 KAPFSQVISIE-STNTKKGKILFDVNVSSWRNSDGGKGQQPYKSLPGDIFVILDADPQTI 143
+A + I+ + K + L+ N KG Q + D+ + D P I
Sbjct: 82 RAQAFKFWEIKPGKDFKPPRDLYYEVTLQMTNEYMTKGGQNLLEV-NDLIAVTDKRP--I 141
Query: 144 TSDYLEKSSKLNWAFAWLGQLNDNN---------------TPTHLNLHISNNMDQLNSTP 203
D L S + + A + +N+NN + + S
Sbjct: 142 RIDDLRFSHE-PYLLALVCGVNENNPHLITILASKPIIFDDDDDIKTSSKRGKGERKSLS 201
Query: 204 LFIVFLMNLTTNLRIWKTLQCSSDGGIVKHI----LGTTSIDNKTCKQCNGNDGEDYSTQ 263
F V L+N+ TN+RIW L + +GG +K I +D +C C N S
Sbjct: 202 FFGVNLINMMTNIRIWTALHPNPEGGNLKLISRVLQSNNEVDGGSCVSCKENSESVVSDY 261
Query: 264 NFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWKILTI 323
+ LR S LN SQ AI C++ C H +I+LIWGPPGTGKTKTTS+LL L +
Sbjct: 262 SARMLR--SFKLNSSQEDAILRCLEAKSCNHSNNIKLIWGPPGTGKTKTTSVLLLNFLKM 321
Query: 324 NHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFDSHDQ 383
+ RTL CAPTN+A+ + S++V L+ +SL + LG+++LFGNK+R+K D +
Sbjct: 322 --RCRTLTCAPTNIAVLEVCSRLVKLVS-ESLRFDGY--GLGDIVLFGNKERMKIDDRED 381
Query: 384 LKDIYLDRRVEKLFKC-LGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFL------- 443
L D++L+ RV++L++C + G + + MI + + K + ++ +N + L
Sbjct: 382 LFDVFLEYRVDELYRCFMALTGWRANVNRMICLLSDPK-HEFRQFKSVNTTLLSFKDFVE 441
Query: 444 -------LDCVHIFTT---HIPKQVIMEHNWKKLEILVGFICDIGTL-LSKDNY------ 503
D H FTT H+P ++ +K+ + +I + +D Y
Sbjct: 442 ERLSRLRYDLHHQFTTLCLHLPTSLLSFRVAEKMNQTNNLLRNIAASDVMRDGYGRMKYK 501
Query: 504 --DDDDDDDKMGEALIDLKCHCLLVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFS 563
D D++D + CL +L ++ +S I++P +SK ++K C A L+F
Sbjct: 502 LKDTGDENDSRTQ-------DCLEMLTSISMS---IKLPDFISKFELQKLCLDNAYLLFC 561
Query: 564 TASNSFKLNSVKKNSLNLVVVDEAAQLKECESLIPLQLQHINHALLVGDEFQLPATIKSK 623
TAS+S +L+ + + L+V+DEAAQLKECES IPLQL+ + HA+L+GDE QLPA IKS
Sbjct: 562 TASSSARLH--MSSPIQLLVIDEAAQLKECESAIPLQLRGLQHAILIGDEKQLPAMIKSN 621
Query: 624 VCEGAKFGRSLYERLSLMGYSKHLLDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYE 683
+ A GRSL+ERL L+G++K LL+ QYRMHP +S FPN +FY KI+DA V + YE
Sbjct: 622 IASEADLGRSLFERLVLLGHNKQLLNMQYRMHPSISIFPNREFYDMKILDAPSVRLRSYE 681
Query: 684 KNYLPSPLFGPYSFINVCGGQEESNGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISI 743
K +LP ++GPYSFIN+ G+E+ G+G S KN VEV VV +IV LY K IS+
Sbjct: 682 KKFLPEKMYGPYSFINIAYGREQF-GEGYSSKNLVEVSVVAEIVSKLYSVSRKTGRTISV 741
Query: 744 GVISPYNAQVSSIQEKLGRKYEKNNNEG-FRVKVKSIDGFQGGEEDVIIISTVRSNSGHN 803
GVISPY AQV +IQE++G KY N EG F V V+S+DGFQGGEED+IIISTVRSN
Sbjct: 742 GVISPYKAQVFAIQERIGEKY---NTEGTFTVSVRSVDGFQGGEEDIIIISTVRSNGNGA 801
Query: 804 IGFLSNKQRTNVALTRARFCLWIVGDAKTLGKSNSEWRDVINDAKTRQCFFNVEENKELA 816
IGFLSN+QRTNVALTRAR+CLWI+G+ TL + S WR +++DAK R CF N EE++ LA
Sbjct: 802 IGFLSNQQRTNVALTRARYCLWILGNEATLTNNRSVWRQLVDDAKARNCFHNAEEDESLA 833
BLAST of MELO3C020052.jh1 vs. TAIR 10
Match:
AT1G65810.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )
HSP 1 Score: 533.1 bits (1372), Expect = 4.1e-151
Identity = 334/840 (39.76%), Postives = 482/840 (57.38%), Query Frame = 0
Query: 24 LIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYKASYIFPLLEETRAELCSNLKTIQ 83
L+D +FSW +V N N Y+ +V KIP +F + ++Y S++ P++EET A+L S++ TI+
Sbjct: 22 LVDVVFSWSLRDVLNSNLYRGQVGKIPNTFTSTKEYFESFVKPIIEETHADLLSSMGTIR 81
Query: 84 KAPFSQVISIE-STNTKKGKILFDVNVSSWRNSDGGKGQQPYKSLPGDIFVILDADPQTI 143
+A + I+ + K + L+ N KG Q + D+ + D P I
Sbjct: 82 RAQAFKFWEIKPGKDFKPPRDLYYEVTLQMTNEYMTKGGQNLLEV-NDLIAVTDKRP--I 141
Query: 144 TSDYLEKSSKLNWAFAWLGQLNDNN---------------TPTHLNLHISNNMDQLNSTP 203
D L S + + A + +N+NN + + S
Sbjct: 142 RIDDLRFSHE-PYLLALVCGVNENNPHLITILASKPIIFDDDDDIKTSSKRGKGERKSLS 201
Query: 204 LFIVFLMNLTTNLRIWKTLQCSSDGGIVKHI----LGTTSIDNKTCKQCNGNDGEDYSTQ 263
F V L+N+ TN+RIW L + +GG +K I +D +C C N S
Sbjct: 202 FFGVNLINMMTNIRIWTALHPNPEGGNLKLISRVLQSNNEVDGGSCVSCKENSESVVSDY 261
Query: 264 NFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWKILTI 323
+ LR S LN SQ AI C++ C H +I+LIWGPPGTGKTKTTS+LL L +
Sbjct: 262 SARMLR--SFKLNSSQEDAILRCLEAKSCNHSNNIKLIWGPPGTGKTKTTSVLLLNFLKM 321
Query: 324 NHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFDSHDQ 383
+ RTL CAPTN+A+ + S++V L+ +SL + LG+++LFGNK+R+K D +
Sbjct: 322 --RCRTLTCAPTNIAVLEVCSRLVKLVS-ESLRFDGY--GLGDIVLFGNKERMKIDDRED 381
Query: 384 LKDIYLDRRVEKLFKC-LGQHGLKFQITSMIGIFQENKLSKMKRMFKLNASFL------- 443
L D++L+ RV++L++C + G + + MI + + K + ++ +N + L
Sbjct: 382 LFDVFLEYRVDELYRCFMALTGWRANVNRMICLLSDPK-HEFRQFKSVNTTLLSFKDFVE 441
Query: 444 -------LDCVHIFTT---HIPKQVIMEHNWKKLEILVGFICDIGTL-LSKDNY------ 503
D H FTT H+P ++ +K+ + +I + +D Y
Sbjct: 442 ERLSRLRYDLHHQFTTLCLHLPTSLLSFRVAEKMNQTNNLLRNIAASDVMRDGYGRMKYK 501
Query: 504 --DDDDDDDKMGEALIDLKCHCLLVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFS 563
D D++D + CL +L ++ +S I++P +SK ++K C A L+F
Sbjct: 502 LKDTGDENDSRTQ-------DCLEMLTSISMS---IKLPDFISKFELQKLCLDNAYLLFC 561
Query: 564 TASNSFKLNSVKKNSLNLVVVDEAAQLKECESLIPLQLQHINHALLVGDEFQLPATIKSK 623
TAS+S +L+ + + L+V+DEAAQLKECES IPLQL+ + HA+L+GDE QLPA IKS
Sbjct: 562 TASSSARLH--MSSPIQLLVIDEAAQLKECESAIPLQLRGLQHAILIGDEKQLPAMIKSN 621
Query: 624 VCEGAKFGRSLYERLSLMGYSKHLLDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYE 683
+ A GRSL+ERL L+G++K LL+ QYRMHP +S FPN +FY KI+DA V + YE
Sbjct: 622 IASEADLGRSLFERLVLLGHNKQLLNMQYRMHPSISIFPNREFYDMKILDAPSVRLRSYE 681
Query: 684 KNYLPSPLFGPYSFINVCGGQEESNGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISI 743
K +LP ++GPYSFIN+ G+E+ G+G S KN VEV VV +IV LY K IS+
Sbjct: 682 KKFLPEKMYGPYSFINIAYGREQF-GEGYSSKNLVEVSVVAEIVSKLYSVSRKTGRTISV 741
Query: 744 GVISPYNAQVSSIQEKLGRKYEKNNNEG-FRVKVKSIDGFQGGEEDVIIISTVRSNSGHN 803
GVISPY AQV +IQE++G KY N EG F V V+S+DGFQGGEED+IIISTVRSN
Sbjct: 742 GVISPYKAQVFAIQERIGEKY---NTEGTFTVSVRSVDGFQGGEEDIIIISTVRSNGNGA 801
Query: 804 IGFLSNKQRTNVALTRARFCLWIVGDAKTLGKSNSEWRDVINDAKTRQCFFNVEENKELA 816
IGFLSN+QRTNVALTRAR+CLWI+G+ TL + S WR +++DAK R CF N EE++ LA
Sbjct: 802 IGFLSNQQRTNVALTRARYCLWILGNEATLTNNRSVWRQLVDDAKARNCFHNAEEDESLA 833
BLAST of MELO3C020052.jh1 vs. TAIR 10
Match:
AT5G37150.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )
HSP 1 Score: 523.5 bits (1347), Expect = 3.2e-148
Identity = 320/841 (38.05%), Postives = 476/841 (56.60%), Query Frame = 0
Query: 20 ESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYKASYIFPLLEETRAELCSNL 79
E L+D +FSW ++ N++FYK K +P F + ++Y ++ LL E EL S+L
Sbjct: 5 EKTSLVDRVFSWSIKDILNKDFYKQKT--VPDKFRSVDEYYQCFVPHLLIEAHTELFSSL 64
Query: 80 KTIQKAPFSQVISIE-----STNTKKGKILFDVNVSSWRNSDGGKGQQPYKSLPGDIFVI 139
K++ K+PF Q+ S+E S+ + K+ +D+ + + + Y+ GD+ +
Sbjct: 65 KSVSKSPFVQIRSMETKTKQSSGSSSNKLFYDITLKATESLSA-----KYQPKCGDLIAL 124
Query: 140 LDADPQTITSDYLEKSSKLNWAFAWLGQLNDNNTPTHLNLHISNNMDQLNSTPLFIVFLM 199
P+ I L + F+ G L +++H+S ++ L + F VFLM
Sbjct: 125 TMDKPRRIND---LNPLLLAYVFSSDGDLK-------ISVHLSRSISPLENYS-FGVFLM 184
Query: 200 NLTTNLRIWKTLQC-SSDGGIVKHILGTTSIDNKTCKQCNGNDGEDYSTQNFPTLRLPSL 259
LTTN RIW L ++ + K +L +++N + G+ T +R S
Sbjct: 185 TLTTNTRIWNALHNEAAISTLTKSVLQANTVNNVFVLKMMGD-----LTLFLDIIR--ST 244
Query: 260 SLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWKILTINHQIRTLACA 319
LN SQ AI C++ C HK S++LIWGPPGTGKTKT + LL+ +L + + +T+ CA
Sbjct: 245 KLNSSQEDAILGCLETRNCTHKNSVKLIWGPPGTGKTKTVATLLFALLKL--RCKTVVCA 304
Query: 320 PTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFDSHDQ-LKDIYLDRR 379
PTN AI +AS++++L K +S S+N + LG ++L GN+DR+ +D L D++LD R
Sbjct: 305 PTNTAIVQVASRLLSLFKENSTSENATY-RLGNIILSGNRDRMGIHKNDHVLLDVFLDER 364
Query: 380 VEKLFKCLGQHGLKFQITSMIGIFQEN----------KLSKMKRM--------------- 439
+ KL K Q + F EN +L +++RM
Sbjct: 365 IGKLGKLFSPFSGWMQRLESLIQFLENPEGKYERHVYELEEVERMEEEAERQEVVVNIPT 424
Query: 440 ----FKLNASFLLD----CVHIFTTHIPKQVIMEHNWKKLEILVGFICDIGTLLSKDNYD 499
K N + L + C+ TH+PK V + ++ K+ I ++N
Sbjct: 425 IGEFVKKNFNSLSEEVETCIVDLFTHLPK-VYLPYDDVKIMIASRQSLQRIRYFLRENSS 484
Query: 500 --DDDDDDKMGEALIDLKCHCLLVLRTLLVSLDEIEVPSKLSKNSIEKFCFQKASLIFST 559
D ++ + + L CL LR L E+P L I KFC Q A +I T
Sbjct: 485 RVDFEEGNFRFDCFKRLSVDCLKALRLL---PKRFEIPDMLENEDIRKFCLQNADIILCT 544
Query: 560 ASNSFKLNSVKKNSLNLVVVDEAAQLKECESLIPLQLQHINHALLVGDEFQLPATIKSKV 619
AS + ++N + ++ L+VVDEAAQLKECES+ LQL + HA+L+GDEFQLPA + +++
Sbjct: 545 ASGAAEMNVERTGNVELLVVDEAAQLKECESVAALQLPGLRHAILIGDEFQLPAMVHNEM 604
Query: 620 CEGAKFGRSLYERLSLMGYSKHLLDTQYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEK 679
CE AKFGRSL+ERL L+G++KHLLD QYRMHP +S FPN +FYG +I DA V Y+K
Sbjct: 605 CEKAKFGRSLFERLVLLGHNKHLLDVQYRMHPSISRFPNKEFYGGRIKDAENVKESIYQK 664
Query: 680 NYLPSPLFGPYSFINVCGGQEESNGDGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISIG 739
+L +FG +SFINV G+EE GDG S KN VEV VV++I+ L+K C+ + +S+G
Sbjct: 665 RFLQGNMFGSFSFINVGRGKEEF-GDGHSPKNMVEVAVVSEIISNLFKVSCERRMKVSVG 724
Query: 740 VISPYNAQVSSIQEKLGRKYEKNNNEGFRVKVKSIDGFQGGEEDVIIISTVRSNSGHNIG 799
V+SPY Q+ +IQEK+G KY + + F + V+S+DGFQGGEED+IIISTVRSNS +G
Sbjct: 725 VVSPYKGQMRAIQEKIGDKYSSLSGQQFALNVRSVDGFQGGEEDIIIISTVRSNSNGKVG 784
Query: 800 FLSNKQRTNVALTRARFCLWIVGDAKTLGKSNSEWRDVINDAKTRQCFFNVEENKELANE 819
FL+N+QR NVALTRAR CLW++G+ TL S S W +I++++TR CF++ + L N
Sbjct: 785 FLNNRQRANVALTRARHCLWVIGNETTLALSGSIWATLISESRTRGCFYDATDEMNLRNA 812
BLAST of MELO3C020052.jh1 vs. TAIR 10
Match:
AT1G65780.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )
HSP 1 Score: 483.0 bits (1242), Expect = 4.8e-136
Identity = 312/894 (34.90%), Postives = 468/894 (52.35%), Query Frame = 0
Query: 20 ESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYKASYIFPLLEETRAELCSNL 79
E L+D + SW + V N + YK +V KIP FE+ Y ++I PL+EET A L S++
Sbjct: 7 EGRDLVDLVLSWSLDEVLNVDLYKGQVEKIPMEFESTGDYFKTFIPPLIEETHAALLSSM 66
Query: 80 KTIQKAPFSQVISIESTNTKK--GKILFDVNVSSWRNSDGGKGQQPYKSLPGDIFVILDA 139
+ + +AP ++ I T K + + V +S G + K +P D+ + D
Sbjct: 67 RKLWQAPVVEISYIMQTAEYKLPNDLFYKVRLS------GISNEASTKLMPRDLISLTDQ 126
Query: 140 DPQTITSDYLEKSSKLNWAFAWL--GQLNDNNTPTHLNLHISNNMDQLNSTP--LFIVFL 199
P + + + + + ND L + + + N LF + L
Sbjct: 127 RPNHVDGFNISSEPYIVALVCKVDPDRPNDVTILASKPLFVEDGRRKKNEKKERLFGIHL 186
Query: 200 MNLTTNLRIWKTLQCSSDG---GIVKHILGTTSIDNKTCKQCNGNDGEDYSTQNFPTLRL 259
+NLTTN+RIW L +G ++ +L S D C QC + + + F
Sbjct: 187 VNLTTNIRIWNALHPGDEGVNLNLISRVLRRNSEDEGFCIQCLQEGSDGLAPRRF----- 246
Query: 260 PSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWKILTINHQIRTL 319
L LN SQ AI +C+ C H ++ LIWGPPGTGKTKTTS+LL+ +L N + RTL
Sbjct: 247 --LKLNPSQEDAILNCLDVRRCYHANTVRLIWGPPGTGKTKTTSVLLFTLL--NAKCRTL 306
Query: 320 ACAPTNVAITNLASQVVNLLKHDSLSKNDVFCPLGELLLFGNKDRLKFDSHDQLKDIYLD 379
C PTNV++ +AS+V+ L+ N LG+++LFGN +R+K L +I++D
Sbjct: 307 TCGPTNVSVLEVASRVLKLVSGSLKIGN---YGLGDVVLFGNDERMKIKDRKDLVNIFID 366
Query: 380 RRVEKLFKC-LGQHGLKFQITSMIGIFQE------------------------------- 439
RV+KL+ C + +G K I MI + ++
Sbjct: 367 ERVDKLYPCFMPFYGWKATIDGMIRLLEDPKGQYNLYLENLARANNVKRKDTGSVFKRKG 426
Query: 440 ------------------------NKLSKMKRMFKLNASFLLDCVHIFTTHIPKQVIMEH 499
K S++++ L+ S L TH+P ++
Sbjct: 427 NEQNENIVEQVSDTRPQSFQDYLPEKFSELRKDLDLHFSSL-------CTHLPTALLSSQ 486
Query: 500 NWKKLEILVGFICDIGTLLSKDNYDDD----------DDDDKMGEALIDLKCHCLLVLRT 559
++ + + D+ L D + + D+ + ++ L +LR
Sbjct: 487 AATRMYEAIDLVRDVTILAILDGVTGEGVKSVLIPNGEGSDRFSSQHVTVEDDYLKLLR- 546
Query: 560 LLVSLDEI-EVPSKLSKNSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVDEAAQL 619
S+ EI +P+ ++ I++ C A L+FSTAS S +L + + L+V+DEAAQL
Sbjct: 547 ---SIPEIFPLPAVSDRHLIKELCLGHACLLFSTASCSARLYT--GTPIQLLVIDEAAQL 606
Query: 620 KECESLIPLQLQHINHALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSKHLLDT 679
KECES IP+QL + H +LVGDE QLPA ++S++ A FGRSL+ERL+L+G+ K++L+
Sbjct: 607 KECESSIPMQLPGLRHLILVGDERQLPAMVESQIALEAGFGRSLFERLALLGHKKYMLNI 666
Query: 680 QYRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEE-SNG 739
QYRMH +S FPN + YG KI+DA V + Y K YLP ++GPYSFIN+ G+EE G
Sbjct: 667 QYRMHCSISSFPNKELYGKKILDAPTVRQRNYTKQYLPGEMYGPYSFINIAYGREEYGEG 726
Query: 740 DGQSKKNTVEVIVVTQIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEKNNN 799
+G+S KN VEV+VV I+ L + K K I++GVISPY AQV +IQEK+ +
Sbjct: 727 EGRSLKNNVEVVVVAAIIANLLQVSEKTKTRINVGVISPYKAQVIAIQEKIQETSIGDAG 786
Query: 800 EGFRVKVKSIDGFQGGEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLWIVGDA 828
F ++++++DGFQGGEED+II+STVRSN +GFL N++RTNV LTRARFCLWI+G+
Sbjct: 787 GLFSLRIRTVDGFQGGEEDIIIVSTVRSNGVGRVGFLGNRRRTNVLLTRARFCLWILGNE 846
BLAST of MELO3C020052.jh1 vs. TAIR 10
Match:
AT5G37160.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )
HSP 1 Score: 481.1 bits (1237), Expect = 1.8e-135
Identity = 311/875 (35.54%), Postives = 473/875 (54.06%), Query Frame = 0
Query: 14 KASNHKESNGLIDALFSWDFNNVFNQNFYKLKVRKIPKSFETEEQYKASYIFPLLEETRA 73
K E L L SW ++ N++ K K+ IP F + ++Y ++ LLEETR
Sbjct: 8 KLLEKNEQTSLFARLCSWSLKDILNEDLSKEKIMTIPDRFSSVDEYSQCFVPHLLEETRT 67
Query: 74 ELCSNLKTIQKAPFSQVISIES-----TNTKKGKILFDVNVSSWRNSDGGKGQQPYKSLP 133
EL S+ +++ K+P S+++S+E+ + K D+ + + + + Y+
Sbjct: 68 ELFSSFRSLSKSPVSRILSVETKVIEYSGRSSIKWFHDIKLMDYADDK----NEIYEPKC 127
Query: 134 GDIFVILDADPQTITSDYLEKSS----KLNWAFAWLGQLNDNNTPTHLNLHISNNMDQLN 193
GDI + P ++T + L + F+ G D+ H + IS +
Sbjct: 128 GDIIAL---SPLSLTEERPRIDDLDPLLLGYVFSVYG---DSKISVHFSRSISQSEKHTF 187
Query: 194 STPLFIVFLMNLTTNLRIWKTL-QCSSDGGIVKHILGTTSIDNKTCKQC-NGNDGEDYST 253
T VFL+N+TTN RIW L + ++D +++ +L + + C C N DG D S
Sbjct: 188 CTG---VFLINITTNTRIWNALHKDAADSTLIQSVLQEDASATEQCFSCENDVDGSD-SD 247
Query: 254 QNFPTLRLPSLSLNESQRVAIESCIKKVICQHKPSIELIWGPPGTGKTKTTSILLWKILT 313
+ +R S LN SQ AI +K C+HK S++LIWGPPGTGKTKT + LL ++
Sbjct: 248 RVVDIIR--SAKLNSSQEAAILGFLKTRNCKHKESVKLIWGPPGTGKTKTVATLLSTLMQ 307
Query: 314 INHQIRTLACAPTNVAITNLASQVVNLLKHDSLSKNDVFCP------------------- 373
+ + +T+ CAPTN I +AS+++ SLSK + C
Sbjct: 308 L--KCKTVVCAPTNTTIVAVASRLL------SLSKETIVCAPTNSAIAEVVSRFEFSTLF 367
Query: 374 ------------LGELLLFGNKDRLKFDSHDQLKDIYLDRRVEKLFKC-LGQHGLKFQIT 433
+G ++L GN++R+ S+ L +++ + RV KL + L G K ++
Sbjct: 368 YGTSILERTTYGMGNIVLSGNRERMGITSNKVLLNVFFNDRVSKLGRLFLSTCGWKKRLE 427
Query: 434 SMIGIFQENKLSKMKRMFKLNASFLLD----------------CVHIFTTHIPKQVIMEH 493
S+I + + + + +L + + + +TH+PK I
Sbjct: 428 SIIDFLENTETKYEQHVNELELERMTEDEKKKEEVEERTMQEVDMADLSTHLPKSFISSK 487
Query: 494 NWKKLEILVGFICDIGTLLSKDNYDDDDDDDKMGEALIDLKCHCLLVLRTLLVSLDEIEV 553
+ K L + + L +++ DD K G + +C L+S+D ++
Sbjct: 488 DVKNLIAACQALHRVRYFLQENS---SRDDFKKG----GFRFNCF----NKLISVDALQA 547
Query: 554 PSKLSK----------NSIEKFCFQKASLIFSTASNSFKLNSVKKNSLNLVVVDEAAQLK 613
L K I KFC Q A +IF TAS+ +N + S++L+VVDE AQLK
Sbjct: 548 LCLLPKCFGIFGLANNEDIRKFCLQNADIIFCTASSVANINPARIGSVDLLVVDETAQLK 607
Query: 614 ECESLIPLQLQHINHALLVGDEFQLPATIKSKVCEGAKFGRSLYERLSLMGYSKHLLDTQ 673
ECES+ LQL + HALL+GDE+QLPA + ++ C+ AKFGRSL+ERL L+G+SKHLL+ Q
Sbjct: 608 ECESVAALQLPGLCHALLIGDEYQLPAMVHNEECDKAKFGRSLFERLVLIGHSKHLLNVQ 667
Query: 674 YRMHPFVSYFPNSKFYGNKIMDASIVMNKGYEKNYLPSPLFGPYSFINVCGGQEESNGDG 733
YRMHP +S FPN +FYG +I DA+ V YEK +L +FG +SFINV G+EE GDG
Sbjct: 668 YRMHPSISRFPNKEFYGGRITDAANVQESIYEKRFLQGNMFGTFSFINVGRGKEEF-GDG 727
Query: 734 QSKKNTVEVIVVTQIVQMLYKAWCKNKNDISIGVISPYNAQVSSIQEKLGRKYEK-NNNE 793
S KN VEV V+++I+ L+K + K +S+GVISPY QV +IQE++G KY + ++
Sbjct: 728 HSPKNMVEVAVISKIISNLFKVSSQRKQKMSVGVISPYKGQVRAIQERVGDKYNSLSVDQ 787
Query: 794 GFRVKVKSIDGFQGGEEDVIIISTVRSNSGHNIGFLSNKQRTNVALTRARFCLWIVGDAK 819
F + V+S+DGFQGGE DVIIISTVR N N+GFLSN+QR NVALTRAR CLW++G+
Sbjct: 788 LFTLNVQSVDGFQGGEVDVIIISTVRCNVNGNVGFLSNRQRANVALTRARHCLWVIGNGT 846
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q00416 | 3.8e-45 | 25.24 | Helicase SEN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292... | [more] |
B6SFA4 | 4.2e-44 | 34.82 | Probable helicase MAGATAMA 3 OS=Arabidopsis thaliana OX=3702 GN=MAA3 PE=2 SV=1 | [more] |
Q92355 | 2.2e-37 | 34.45 | Helicase sen1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 G... | [more] |
O94387 | 8.5e-37 | 33.52 | Uncharacterized ATP-dependent helicase C29A10.10c OS=Schizosaccharomyces pombe (... | [more] |
Q86AS0 | 3.2e-36 | 34.07 | Probable helicase DDB_G0274399 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3C4A0 | 0.0 | 98.70 | helicase SEN1-like OS=Cucumis melo OX=3656 GN=LOC103496680 PE=4 SV=1 | [more] |
A0A0A0KMQ3 | 0.0 | 89.76 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G292250 PE=4 SV=1 | [more] |
A0A6J1JC41 | 0.0 | 72.49 | helicase sen1-like OS=Cucurbita maxima OX=3661 GN=LOC111485435 PE=4 SV=1 | [more] |
A0A6J1FQ96 | 0.0 | 72.73 | helicase SEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111447261 PE=4 SV=1 | [more] |
A0A6J1DR27 | 0.0 | 67.96 | helicase SEN1-like OS=Momordica charantia OX=3673 GN=LOC111023039 PE=4 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT1G65810.1 | 4.1e-151 | 39.76 | P-loop containing nucleoside triphosphate hydrolases superfamily protein | [more] |
AT1G65810.2 | 4.1e-151 | 39.76 | P-loop containing nucleoside triphosphate hydrolases superfamily protein | [more] |
AT5G37150.1 | 3.2e-148 | 38.05 | P-loop containing nucleoside triphosphate hydrolases superfamily protein | [more] |
AT1G65780.1 | 4.8e-136 | 34.90 | P-loop containing nucleoside triphosphate hydrolases superfamily protein | [more] |
AT5G37160.1 | 1.8e-135 | 35.54 | P-loop containing nucleoside triphosphate hydrolases superfamily protein | [more] |