MELO3C019216 (gene) Melon (DHL92) v4

Overview
NameMELO3C019216
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionCytochrome P450 81D1-like
Locationchr11: 9185563 .. 9188176 (-)
RNA-Seq ExpressionMELO3C019216
SyntenyMELO3C019216
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTTGATCACTTGCTTTACACTTTGCTCTCTCTCATTTCTCTTCTACTTGGCTACTCCTTTTTATTTAAACCTCATCGCTTAAACCTCCCGCCAACTCCACTTTTTGGTCTTCCATTCATAGGTCATCTCCATCTTCTCAAACATCCTGTCCACAAAACATTCCAAACCCTCTCCCAAAAGTACGGCCATGTCTTCTCTCTCAAATTCGGTTCTCGTCTCGTCGTACTTGTATCTTCTCCTTCTGCCATCCAAGAATGTTTCACAAAGAATGATATCATTCTCGCAAATCGGCCATCTTTGAACTCTGGAAAGTACTTAGCATACAACAATACCACCATGGTGGTGTCCTCTTATGGTAAACATTGGCGAAACCTCCGCCGAATTAGTACCCTCGAGATTTTCTCGACAACTCGTCTTAATTCGTTCTCAAGAACTCGGGAAGAGGAAGTCAAACGTTTACTACGTAAATTATGGGGTAATTATAAGTTGGAAGATGAGTTTAGGGTTGTGGAATTGGAGCCCATGTTGTTGGATCTTACATTCAATATTGTAATGAGAATGGTGGGTGGAAAGAAGTTTTGCGAAGAGAAAAATAATAATGTGTTGGAGGATGAGGGATATTGCAAAAGATTTAAAGAGTTGGTGACACAAATAATGGCACATGGTGGATCAACAAATCCAGGGGATTTCATACCTTTATTGAATTGGATTGATCCAAGTGGTTATACGAAAAGGATATAATGAAGATTGGAAGAAAAACAGATGAAGTTCTTCAAGGATTAGTTGATGAGATTAGGAATGAAGAAGATGAAGGAAACACTATGATTCAACATTTACTTCGTTTGCAGAAAACCGATCCCGAATATTATAGTGACCAAATTATCAAAGGTCTCGTACAAGTATGTTTTTCTTTCTAAATTAACATCTAAGTTTGATTTTCCAATATATAAACAATTTTTGTCGATCGTCAAAGAGTAACAATATGATCTTTTCATTTTTTATTTTACACCTTTAGTAATTTCTAAAGAGATTTAAGCTTCTGACTACTTGGGTTTTTGTTTCAGAAGTTTTCCTTGTTTCCCATCATTTATTGCATAGAGTTATAGACTTCTATTAACAGATTTTGCTATATTTACAATTTTTTGAAAATATTACTATATACTTAATTATTTTGAATCTAATTGCTTTATTTCCAACTATCGGTAAAATAAACATTAAAATCTATTTTGAACATTGTTTTCTAAAAACTCCAATTTTCTCTGAAATTAATTCTTTTTTAATTAAAAACTTGAGAATATATTTGAAGAATTTTCCAAACTAACATGCTCATGTATTTGTCATATAAAACTAGCACCTTTTTTAAAAAAATAGTTCAAATAAATTTTTTCAATCCATCTCGAACGAGATTGACCACAAAAGTTTTGGTAAAAATTAAAATTTTCTAACTTATTGATTCTTTTTAACCTTCTCAGTACATCTTGGTGGCAATTACATCGAACTTTTAGTAATTTCTCAAATCTTATCAAATCGTATACCAAATCTTATATGTTTTTATGATTTTTCTAAATTTGGCTATTTTGTCGTATATTATGTGCAATTCTTGCATTCTATAGAAATCAATTTAGAGATCTAAACAATTACTAATATATGATATTTTGTAAATATAACTGAATTTAAAAAAGTTGAAAATTATAATGAGGTTTGATATTAGATTTGGGAATATTTTAGAATCTCTGTCTAATCTCTATCGAGATGTAGCCTAAAACAATCAATAACAAACCAAATCTATCCCTCAAATTACTCCATCACTTCTTCCAACGAGTTTTCAACTTTTGCAGGATATAATATTAGCCGGAATTGACACATCAACTATATCTTTGGAGTGGGCACTATCTCAATTGCTAAATAATCCAGAGGTATTAAAGAAAGCAAAAGAAGAGATAGATTGTTTGATTGGGCATAAACGTCTAGTTAGTGAAGTAGATCTACCAAGTTTGAGTTACCTTCAAGGAATAATCTCTGAGACTTTACGACTTCATCCACCTGCTCCTCTTCTCGTGCCGCATTGTGCATTATTTGAAGAGTGCAAAATAAAAAGATACAATATTCCACGTGATACAATTGTATTGATTAATGCTTGGGCTATCCATAGAGATTCAGGTCTTTGGGAAGATGCCACTGAGTTTAAACCTGAAAGGCATAGTGTGATTGAAAGTGAGTTATATAAATTAATACCATTTGGAGTGGGGAGGAGAGCATGTCCCGGTGCAGGGATGGCACAACGAGTGGTTGGCTTAGCTTTAGCATCATTGATACAAAGCTTTGAATGGGAAAGAGTAGATGACTCATTGATTGACATGAGTGAAGGTAAAGGACTCACAATGCCCAAAGCTCAACCATTGATCGCTAAGTGAAAACCGTGTCCAATCATGAAAATCATTTTGAATGCACACACAGCAACTAATTAACAAAATTTATATCTAAAGTAAGATTAGCTGCTCAATAATAATTTGTATGGTTTTCTATCTATATATAGAAGTTATACATCGAACATTCTCTCATTTTGTTGTATTGAAGAAATTATAGTGTTTGAATTTAAATATATATA

mRNA sequence

ATGGATTTTGATCACTTGCTTTACACTTTGCTCTCTCTCATTTCTCTTCTACTTGGCTACTCCTTTTTATTTAAACCTCATCGCTTAAACCTCCCGCCAACTCCACTTTTTGGTCTTCCATTCATAGGTCATCTCCATCTTCTCAAACATCCTGTCCACAAAACATTCCAAACCCTCTCCCAAAAGTACGGCCATGTCTTCTCTCTCAAATTCGGTTCTCGTCTCGTCGTACTTGTATCTTCTCCTTCTGCCATCCAAGAATGTTTCACAAAGAATGATATCATTCTCGCAAATCGGCCATCTTTGAACTCTGGAAAGTACTTAGCATACAACAATACCACCATGGTGGTGTCCTCTTATGGTAAACATTGGCGAAACCTCCGCCGAATTAGTACCCTCGAGATTTTCTCGACAACTCGTCTTAATTCGTTCTCAAGAACTCGGGAAGAGGAAGTCAAACGTTTACTACGTAAATTATGGGGTAATTATAAGTTGGAAGATGAGTTTAGGGTTGTGGAATTGGAGCCCATGTTGTTGGATCTTACATTCAATATTGTAATGAGAATGGTGGGTGGAAAGAAGTTTTGCGAAGAGAAAAATAATAATGTGTTGGAGGATGAGGGATATTGCAAAAGATTTAAAGAGTTGTGGTTATACGAAAAGGATATAATGAAGATTGGAAGAAAAACAGATGAAGTTCTTCAAGGATTAGTTGATGAGATTAGGAATGAAGAAGATGAAGGAAACACTATGATTCAACATTTACTTCGTTTGCAGAAAACCGATCCCGAATATTATAGTGACCAAATTATCAAAGGTCTCGTACAAGATATAATATTAGCCGGAATTGACACATCAACTATATCTTTGGAGTGGGCACTATCTCAATTGCTAAATAATCCAGAGGTATTAAAGAAAGCAAAAGAAGAGATAGATTGTTTGATTGGGCATAAACGTCTAGTTAGTGAAGTAGATCTACCAAGTTTGAGTTACCTTCAAGGAATAATCTCTGAGACTTTACGACTTCATCCACCTGCTCCTCTTCTCGTGCCGCATTGTGCATTATTTGAAGAGTGCAAAATAAAAAGATACAATATTCCACGTGATACAATTGTATTGATTAATGCTTGGGCTATCCATAGAGATTCAGGTCTTTGGGAAGATGCCACTGAGTTTAAACCTGAAAGGCATAGTGTGATTGAAAGTGAGTTATATAAATTAATACCATTTGGAGTGGGGAGGAGAGCATGTCCCGGTGCAGGGATGGCACAACGAGTGGTTGGCTTAGCTTTAGCATCATTGATACAAAGCTTTGAATGGGAAAGAGTAGATGACTCATTGATTGACATGAGTGAAGGTAAAGGACTCACAATGCCCAAAGCTCAACCATTGATCGCTAAGTGAAAACCGTGTCCAATCATGAAAATCATTTTGAATGCACACACAGCAACTAATTAACAAAATTTATATCTAAAGTAAGATTAGCTGCTCAATAATAATTTGTATGGTTTTCTATCTATATATAGAAGTTATACATCGAACATTCTCTCATTTTGTTGTATTGAAGAAATTATAGTGTTTGAATTTAAATATATATA

Coding sequence (CDS)

ATGGATTTTGATCACTTGCTTTACACTTTGCTCTCTCTCATTTCTCTTCTACTTGGCTACTCCTTTTTATTTAAACCTCATCGCTTAAACCTCCCGCCAACTCCACTTTTTGGTCTTCCATTCATAGGTCATCTCCATCTTCTCAAACATCCTGTCCACAAAACATTCCAAACCCTCTCCCAAAAGTACGGCCATGTCTTCTCTCTCAAATTCGGTTCTCGTCTCGTCGTACTTGTATCTTCTCCTTCTGCCATCCAAGAATGTTTCACAAAGAATGATATCATTCTCGCAAATCGGCCATCTTTGAACTCTGGAAAGTACTTAGCATACAACAATACCACCATGGTGGTGTCCTCTTATGGTAAACATTGGCGAAACCTCCGCCGAATTAGTACCCTCGAGATTTTCTCGACAACTCGTCTTAATTCGTTCTCAAGAACTCGGGAAGAGGAAGTCAAACGTTTACTACGTAAATTATGGGGTAATTATAAGTTGGAAGATGAGTTTAGGGTTGTGGAATTGGAGCCCATGTTGTTGGATCTTACATTCAATATTGTAATGAGAATGGTGGGTGGAAAGAAGTTTTGCGAAGAGAAAAATAATAATGTGTTGGAGGATGAGGGATATTGCAAAAGATTTAAAGAGTTGTGGTTATACGAAAAGGATATAATGAAGATTGGAAGAAAAACAGATGAAGTTCTTCAAGGATTAGTTGATGAGATTAGGAATGAAGAAGATGAAGGAAACACTATGATTCAACATTTACTTCGTTTGCAGAAAACCGATCCCGAATATTATAGTGACCAAATTATCAAAGGTCTCGTACAAGATATAATATTAGCCGGAATTGACACATCAACTATATCTTTGGAGTGGGCACTATCTCAATTGCTAAATAATCCAGAGGTATTAAAGAAAGCAAAAGAAGAGATAGATTGTTTGATTGGGCATAAACGTCTAGTTAGTGAAGTAGATCTACCAAGTTTGAGTTACCTTCAAGGAATAATCTCTGAGACTTTACGACTTCATCCACCTGCTCCTCTTCTCGTGCCGCATTGTGCATTATTTGAAGAGTGCAAAATAAAAAGATACAATATTCCACGTGATACAATTGTATTGATTAATGCTTGGGCTATCCATAGAGATTCAGGTCTTTGGGAAGATGCCACTGAGTTTAAACCTGAAAGGCATAGTGTGATTGAAAGTGAGTTATATAAATTAATACCATTTGGAGTGGGGAGGAGAGCATGTCCCGGTGCAGGGATGGCACAACGAGTGGTTGGCTTAGCTTTAGCATCATTGATACAAAGCTTTGAATGGGAAAGAGTAGATGACTCATTGATTGACATGAGTGAAGGTAAAGGACTCACAATGCCCAAAGCTCAACCATTGATCGCTAAGTGA

Protein sequence

MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLDLTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELWLYEKDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK
Homology
BLAST of MELO3C019216 vs. NCBI nr
Match: TYJ98343.1 (isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 918.7 bits (2373), Expect = 2.0e-263
Identity = 462/467 (98.93%), Postives = 462/467 (98.93%), Query Frame = 0

Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
           GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELWLYEKDIMKIGRKTDEVLQGLVDE 240
           LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL     DIMKIGRKTDEVLQGLVDE
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL-----DIMKIGRKTDEVLQGLVDE 240

Query: 241 IRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTISLEWALSQLLNN 300
           IRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTISLEWALSQLLNN
Sbjct: 241 IRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTISLEWALSQLLNN 300

Query: 301 PEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPLLVPHCALFEECK 360
           PEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPLLVPHCALFEECK
Sbjct: 301 PEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPLLVPHCALFEECK 360

Query: 361 IKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLIPFGVGRRACPGA 420
           IKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLIPFGVGRRACPGA
Sbjct: 361 IKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLIPFGVGRRACPGA 420

Query: 421 GMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK 468
           GMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK
Sbjct: 421 GMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK 462

BLAST of MELO3C019216 vs. NCBI nr
Match: XP_008455822.2 (PREDICTED: LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cucumis melo])

HSP 1 Score: 914.1 bits (2361), Expect = 5.0e-262
Identity = 464/492 (94.31%), Postives = 466/492 (94.72%), Query Frame = 0

Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
           GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL---------------------WL- 240
           LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL                     W+ 
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240

Query: 241 ---YEKDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
              Y K+IMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV
Sbjct: 241 PSGYTKNIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300

Query: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360
           QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI
Sbjct: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360

Query: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420
           ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE
Sbjct: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420

Query: 421 RHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKG 468
           RHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKG
Sbjct: 421 RHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKG 480

BLAST of MELO3C019216 vs. NCBI nr
Match: KAA0032538.1 (isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 799.3 bits (2063), Expect = 1.8e-227
Identity = 405/494 (81.98%), Postives = 432/494 (87.45%), Query Frame = 0

Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
           G+HWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL---------------------WL- 240
           LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL                     W+ 
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240

Query: 241 ---YEKDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
              Y K IMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV
Sbjct: 241 PSGYTKRIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300

Query: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360
           QDI+LAGIDTS ++L+WALS LLNNP VL+KAK EID  IG +R+V+E DL SLSYLQGI
Sbjct: 301 QDILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGI 360

Query: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420
           ISETLRL P  PLLVPHC+  E+CKI  Y++PR+TIVLINAWAIHRD  LWEDA+ FKPE
Sbjct: 361 ISETLRLSPAGPLLVPHCS-SEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPE 420

Query: 421 RH--SVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEG 468
           RH  +      YKL+PFG+GRRACPG  MAQRV+GL LASL+Q FEW+R+ D L+DM EG
Sbjct: 421 RHVNAAGFENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREG 480

BLAST of MELO3C019216 vs. NCBI nr
Match: XP_004151924.2 (cytochrome P450 81Q32 [Cucumis sativus] >KGN63284.1 hypothetical protein Csa_022055 [Cucumis sativus])

HSP 1 Score: 743.8 bits (1919), Expect = 9.0e-211
Identity = 383/496 (77.22%), Postives = 416/496 (83.87%), Query Frame = 0

Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLV+LVSSPSA+QECFTKNDIILANRPSLNSGKYL+YNNTTM VS Y
Sbjct: 61  QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
           G+HWRNLRRI TLEIFSTTRLNSFSR REEEVKRLL KL GNYK EDEFRVVELE MLLD
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKEL---------------------W 240
           LT NIVMRMVGGKKFC+E  KN NVLEDEGYCKR KEL                     W
Sbjct: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240

Query: 241 L----YEKDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300
           +    Y K IMKIGR+ DEVLQ LVDEIRNEEDEGNTMIQHLLRLQKTDP+YYSD IIKG
Sbjct: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300

Query: 301 LVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQ 360
           L+QDI++AGIDTS ++L+WALS LLNNP VL KAK EID  IG +R+V+EVDL SLSYLQ
Sbjct: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360

Query: 361 GIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFK 420
           GIISETLRL PP PLLVPHCA  E+CKI  Y++PR+TIVLINAWAIHRD  +WEDA  FK
Sbjct: 361 GIISETLRLSPPGPLLVPHCA-SEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFK 420

Query: 421 PERH--SVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMS 468
           PERH  +V     YKL+PFG+GRRACPG  MAQRVVGL LASLIQ FEW+++ + L+DM 
Sbjct: 421 PERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMR 480

BLAST of MELO3C019216 vs. NCBI nr
Match: XP_038902403.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 712.2 bits (1837), Expect = 2.9e-201
Identity = 364/496 (73.39%), Postives = 409/496 (82.46%), Query Frame = 0

Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFD L   LLSL+ LLLGY+ L KP+RLNLPPTPLF LPFIGHLHLLKHPVH+TFQ LS
Sbjct: 1   MDFDILFCILLSLVCLLLGYTSLLKPNRLNLPPTPLFCLPFIGHLHLLKHPVHQTFQNLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRL+VLVSSPS +QECFTKNDIILANRPSLN+GKYLAYNN TM VSSY
Sbjct: 61  QKYGHVFSLKFGSRLIVLVSSPSVVQECFTKNDIILANRPSLNTGKYLAYNNITMAVSSY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGN-YKLEDEFRVVELEPMLL 180
           G+HWRNLRRISTLEIFS TR+NSF RTRE+EVKRLL KL GN Y LED+FR V+L+PMLL
Sbjct: 121 GEHWRNLRRISTLEIFSMTRVNSFLRTREDEVKRLLCKLCGNCYTLEDKFRAVDLKPMLL 180

Query: 181 DLTFNIVMRMVGGKKFCEE-KNNNVLEDEGYCKRFKEL---------------------W 240
           DLTFNIVMRMVGGKKFCEE  NNNVLEDEGY KRF+E+                     W
Sbjct: 181 DLTFNIVMRMVGGKKFCEENNNNNVLEDEGYSKRFREVVTQIMAHSGSTNPEDFIPLWNW 240

Query: 241 L----YEKDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300
           +    + K IMK+GR+ DE+LQGLVDEIRN+EDEGN MI HLLRLQ TD EYYSDQ+IKG
Sbjct: 241 IDPTGFNKRIMKLGRRADEILQGLVDEIRNQEDEGNNMIHHLLRLQNTDLEYYSDQVIKG 300

Query: 301 LVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQ 360
           L+ DI++AGIDT+ ++LEWALS LLNNP VL++AK EIDC IG +R+V+E DL SLSYLQ
Sbjct: 301 LIHDILIAGIDTTAVTLEWALSHLLNNPIVLERAKAEIDCSIGQERMVNEADLSSLSYLQ 360

Query: 361 GIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFK 420
           GIISETLRL PP PLLVPHCA  E+CKI  Y+IPR TIVLIN WAIHRD  LWEDAT FK
Sbjct: 361 GIISETLRLSPPGPLLVPHCA-SEDCKIGGYDIPRKTIVLINVWAIHRDPNLWEDATSFK 420

Query: 421 PERH-SVIESEL-YKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMS 468
           PERH +V E E  YKL+PFG+GRRACPG  MAQR++GL LASLIQ FEW+R   SL+DMS
Sbjct: 421 PERHANVAEVEYSYKLLPFGLGRRACPGMAMAQRIIGLTLASLIQCFEWKRTSKSLVDMS 480

BLAST of MELO3C019216 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 3.3e-131
Identity = 251/490 (51.22%), Postives = 333/490 (67.96%), Query Frame = 0

Query: 6   LLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGH 65
           +LY++ SLI L++ + FL KP + NLPP+P   LP IGHL LLK P+H+T ++ S+   H
Sbjct: 9   VLYSIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 68

Query: 66  -----VFSLKFGSRLVVLVSSPS-AIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSS 125
                V SL+ GSRLV +VSS   A +ECF KND++LANRP +  GK++ YNNT M+ + 
Sbjct: 69  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 128

Query: 126 YGKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLL 185
           YG HWRNLRR+ T+EIFST RLN F   R +EV+RL+ +L  +     +  VVEL+PML+
Sbjct: 129 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRL--SRLAGTKKTVVELKPMLM 188

Query: 186 DLTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELWL--------------------- 245
           DLTFN +MRM+ GK++  E+      DE   KR ++L                       
Sbjct: 189 DLTFNNIMRMMTGKRYYGEETT----DEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF 248

Query: 246 --YEKDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQ 305
             YE  + K+G +TD+ LQGL+D+ R +++ G TMI HLL LQK+D EYY+DQIIKG++ 
Sbjct: 249 SSYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIIL 308

Query: 306 DIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGII 365
            +++AG +TS ++LEWALS LLN+P+V+ KA++EID  +G  RL+ E DL  L YL+ I+
Sbjct: 309 IMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIV 368

Query: 366 SETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPER 425
            ETLRLHP  PLLVPH A  E+CKI  Y++PR T +L+NAWAIHRD   W+D   FKPER
Sbjct: 369 LETLRLHPATPLLVPHMA-SEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPER 428

Query: 426 HSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGL 467
               E E  KL+ FG+GRRACPG+G+AQR+VGLAL SLIQ FEWERV +  +DM EG G 
Sbjct: 429 FEK-EEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGN 488

BLAST of MELO3C019216 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 6.2e-130
Identity = 245/487 (50.31%), Postives = 332/487 (68.17%), Query Frame = 0

Query: 1   MDFDHLLYTLLSLISLLLGYS-FLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTL 60
           M+   LLYT L+++ L +    F     R NLPP+P   LP IGHLHL+   +H++   L
Sbjct: 1   MESSTLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDL 60

Query: 61  SQKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSS 120
           S+KYG VFSL+ G+RLV++VSSP+A +ECFTKNDI+ ANRP    GKY+ YN TTMV S 
Sbjct: 61  SKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSP 120

Query: 121 YGKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLL 180
           YG+HWRNLRR++ +EIFS   LN F   RE+EVK+LL  L+       +F  VE++  L 
Sbjct: 121 YGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLY--QSSGQDFGKVEMKSKLS 180

Query: 181 DLTFNIVMRMVGGKKFCEEKNNN------------VLEDEGYCK--------RFKELWLY 240
           +L+FN+ MRMV GK++  +  ++            V E  G           R+ +   Y
Sbjct: 181 ELSFNVTMRMVAGKRYFGQDVDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNY 240

Query: 241 EKDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDII 300
           EK + KI ++ D  LQ L+ E R  ++   TMI HLL LQ++ PEYY+DQIIKG++  ++
Sbjct: 241 EKKVSKISQEMDAFLQRLIHESRINKN-NVTMIDHLLSLQESQPEYYTDQIIKGIIMVLL 300

Query: 301 LAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISET 360
           LAG DTS +++EWA+S LLN+PE L+KA+ EI+  +G  RL+ E DLP L+YL  IISET
Sbjct: 301 LAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISET 360

Query: 361 LRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSV 420
            RL P AP+LVPH    ++CK++ Y++P+ TI+L+NAWAIHRD   W++ T FKPERH  
Sbjct: 361 FRLCPAAPMLVPH-ESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGG 420

Query: 421 IESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMP 467
           +E E  KL+PFG+GRR+CPG+G+AQRVVGL L +LIQ FEW+R+ ++ IDM+EG GLTMP
Sbjct: 421 VELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMP 480

BLAST of MELO3C019216 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 2.0e-128
Identity = 252/490 (51.43%), Postives = 324/490 (66.12%), Query Frame = 0

Query: 8   YTLLSLISLLLGYSFLFK--PHRL-NLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYG 67
           Y+LLSL S ++    L K    RL NLPP P   +P IG+LH LKHP+H+TF TLSQ YG
Sbjct: 5   YSLLSL-SFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQTYG 64

Query: 68  HVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKHW 127
            +FSL FGSRLVV+VSSPS   ECFTKNDIILANRP   +GKY+ YN TT+  +SYG HW
Sbjct: 65  DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 124

Query: 128 RNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLED-EFRVVELEPMLLDLTF 187
           RNLRRI+T+++ S  RLNSF   R +E  RL++KL  +   E   F  VEL P L ++TF
Sbjct: 125 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTF 184

Query: 188 NIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKE-------------------------LWL 247
           N +MRM+ GK++  +  +  + D    K+F+E                         L  
Sbjct: 185 NAMMRMISGKRYYGDDGD--VSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDN 244

Query: 248 YEKDIMKIGRKTDEVLQGLVDEIR--NEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQ 307
            EK   +I ++++  L+GL++E R  N   +G TMI HLL+L ++ PEYYSD +IKGL+Q
Sbjct: 245 LEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQ 304

Query: 308 DIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGII 367
            ++LAG DTS +++EW +S+LLN+PEVLKKAKEE+D  IG  +LV E DL  L YLQ II
Sbjct: 305 GMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNII 364

Query: 368 SETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPER 427
           SETLRLHPPAPLL+PH +  E+C I  +N+P+DTI+L N W IHRD   W DA  FKPER
Sbjct: 365 SETLRLHPPAPLLLPHYS-SEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPER 424

Query: 428 HSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGL 467
               E E+ K++ FG+GRRACPG  +AQR VG  +  LIQ FEWER  +  +DM EGKG+
Sbjct: 425 FEK-EEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGI 484

BLAST of MELO3C019216 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 3.5e-125
Identity = 249/486 (51.23%), Postives = 319/486 (65.64%), Query Frame = 0

Query: 7   LYTLLSLISLLLGYSFLFKPHR--LNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYG 66
           L  ++SL  L++     F   R   NLPP P   LP IG+LH LK P+H TF TLSQKYG
Sbjct: 6   LSLIISLFFLIITLKVFFNTSRKFKNLPPGPQC-LPIIGNLHQLKQPLHHTFHTLSQKYG 65

Query: 67  HVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKHW 126
            +FSL FGSRLVV+VSS +  QECFTKNDI+LANRP   +GKY+ YNNTT+  S YG HW
Sbjct: 66  QIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHW 125

Query: 127 RNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLDLTFN 186
           RNLRRI ++EI S+ RLNSF   R +E+ RL++KL    K  + F  VEL PM  ++TFN
Sbjct: 126 RNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKL--AQKSYNGFTEVELRPMFSEMTFN 185

Query: 187 IVMRMVGGKKF----CEEKN--------------------NNVLEDEGYCKRFKELWLYE 246
            +MRMV GK++    C+  +                    NNV +  G+ + F    L E
Sbjct: 186 TIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGL-E 245

Query: 247 KDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIIL 306
           K + KI ++TD  LQGL+DE R  +   NTMI HLL  Q++ PEYY+DQIIKGL+  ++L
Sbjct: 246 KRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLL 305

Query: 307 AGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETL 366
           AG DTS++++EWA+S LLN+PE++KKAK E+D  IGH R V E D+  L YLQ I+ ETL
Sbjct: 306 AGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETL 365

Query: 367 RLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVI 426
           RLH  APLLVPH +  E+  +  YNIP++TI+++NAW IHRD  LW D T FKPER    
Sbjct: 366 RLHAAAPLLVPHLS-SEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEK- 425

Query: 427 ESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPK 467
           E E+ KL+ FG+GRRACPG  ++QR  GL L  LIQ FEW+R+ +  IDM E KG+T  K
Sbjct: 426 EGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGK 485

BLAST of MELO3C019216 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 448.7 bits (1153), Expect = 7.8e-125
Identity = 242/488 (49.59%), Postives = 324/488 (66.39%), Query Frame = 0

Query: 10  LLSLISLLLGYSFLFKPHRL--------NLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQ 69
           +LSL+S  + Y  LF    +        NLPP P   LP IG+LH LK P+H+TF+ LS+
Sbjct: 3   ILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGLSE 62

Query: 70  KYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYG 129
           KYGHVFSL FGSRLVV+VSS S  Q+CFTKND++LANRP   SGKY+ YN TT+  +SYG
Sbjct: 63  KYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYG 122

Query: 130 KHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLDL 189
           +HWRNLRRI+ L++ S  R+NSFS  R +E +RL+ +L  +      F  +EL   L D+
Sbjct: 123 EHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADD--SSTNFAEMELSSRLYDM 182

Query: 190 TFNIVMRMVGGKKFC-EEKNNNVLEDEGYCK----------------------RFKELWL 249
           TFN +MRM+ GK++  E+ + + L++    +                      RF +   
Sbjct: 183 TFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFEN 242

Query: 250 YEKDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDI 309
            EK +  I  KTD  L+GL++E R +++  NTMI HLL LQ + PEYY+DQIIKGL   +
Sbjct: 243 LEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAM 302

Query: 310 ILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISE 369
           +LAG D+S ++LEW++S LLN+PEVLKK K+E+D  +G  RLV E DLP L+YL+ +I+E
Sbjct: 303 LLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINE 362

Query: 370 TLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHS 429
           TLRL+ PAPLL+PH +  +EC I  Y +P+DTIVLINAWAIHRD  LW +AT FKPER  
Sbjct: 363 TLRLYTPAPLLLPH-STSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFE 422

Query: 430 VIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTM 467
             + EL KLI FG+GRRACPG G+A R + + LA LIQ F+W+ ++   ID++E  G T+
Sbjct: 423 K-KGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTL 482

BLAST of MELO3C019216 vs. ExPASy TrEMBL
Match: A0A5D3BGH3 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00710 PE=3 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 9.9e-264
Identity = 462/467 (98.93%), Postives = 462/467 (98.93%), Query Frame = 0

Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
           GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELWLYEKDIMKIGRKTDEVLQGLVDE 240
           LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL     DIMKIGRKTDEVLQGLVDE
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL-----DIMKIGRKTDEVLQGLVDE 240

Query: 241 IRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTISLEWALSQLLNN 300
           IRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTISLEWALSQLLNN
Sbjct: 241 IRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTISLEWALSQLLNN 300

Query: 301 PEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPLLVPHCALFEECK 360
           PEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPLLVPHCALFEECK
Sbjct: 301 PEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPLLVPHCALFEECK 360

Query: 361 IKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLIPFGVGRRACPGA 420
           IKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLIPFGVGRRACPGA
Sbjct: 361 IKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLIPFGVGRRACPGA 420

Query: 421 GMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK 468
           GMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK
Sbjct: 421 GMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIAK 462

BLAST of MELO3C019216 vs. ExPASy TrEMBL
Match: A0A1S3C1S2 (LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103495921 PE=3 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 2.4e-262
Identity = 464/492 (94.31%), Postives = 466/492 (94.72%), Query Frame = 0

Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
           GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL---------------------WL- 240
           LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL                     W+ 
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240

Query: 241 ---YEKDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
              Y K+IMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV
Sbjct: 241 PSGYTKNIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300

Query: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360
           QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI
Sbjct: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360

Query: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420
           ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE
Sbjct: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420

Query: 421 RHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKG 468
           RHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKG
Sbjct: 421 RHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKG 480

BLAST of MELO3C019216 vs. ExPASy TrEMBL
Match: A0A5A7SPU0 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold465G00500 PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 8.7e-228
Identity = 405/494 (81.98%), Postives = 432/494 (87.45%), Query Frame = 0

Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
           G+HWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL---------------------WL- 240
           LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL                     W+ 
Sbjct: 181 LTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWID 240

Query: 241 ---YEKDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300
              Y K IMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV
Sbjct: 241 PSGYTKRIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLV 300

Query: 301 QDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGI 360
           QDI+LAGIDTS ++L+WALS LLNNP VL+KAK EID  IG +R+V+E DL SLSYLQGI
Sbjct: 301 QDILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGI 360

Query: 361 ISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPE 420
           ISETLRL P  PLLVPHC+  E+CKI  Y++PR+TIVLINAWAIHRD  LWEDA+ FKPE
Sbjct: 361 ISETLRLSPAGPLLVPHCS-SEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPE 420

Query: 421 RH--SVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEG 468
           RH  +      YKL+PFG+GRRACPG  MAQRV+GL LASL+Q FEW+R+ D L+DM EG
Sbjct: 421 RHVNAAGFENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREG 480

BLAST of MELO3C019216 vs. ExPASy TrEMBL
Match: A0A0A0LQI5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 4.3e-211
Identity = 383/496 (77.22%), Postives = 416/496 (83.87%), Query Frame = 0

Query: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60
           MDFDHLLY LLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKT QTLS
Sbjct: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60

Query: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120
           QKYGHVFSLKFGSRLV+LVSSPSA+QECFTKNDIILANRPSLNSGKYL+YNNTTM VS Y
Sbjct: 61  QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120

Query: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 180
           G+HWRNLRRI TLEIFSTTRLNSFSR REEEVKRLL KL GNYK EDEFRVVELE MLLD
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLD 180

Query: 181 LTFNIVMRMVGGKKFCEE--KNNNVLEDEGYCKRFKEL---------------------W 240
           LT NIVMRMVGGKKFC+E  KN NVLEDEGYCKR KEL                     W
Sbjct: 181 LTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNW 240

Query: 241 L----YEKDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300
           +    Y K IMKIGR+ DEVLQ LVDEIRNEEDEGNTMIQHLLRLQKTDP+YYSD IIKG
Sbjct: 241 IDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKG 300

Query: 301 LVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQ 360
           L+QDI++AGIDTS ++L+WALS LLNNP VL KAK EID  IG +R+V+EVDL SLSYLQ
Sbjct: 301 LIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQ 360

Query: 361 GIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFK 420
           GIISETLRL PP PLLVPHCA  E+CKI  Y++PR+TIVLINAWAIHRD  +WEDA  FK
Sbjct: 361 GIISETLRLSPPGPLLVPHCA-SEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFK 420

Query: 421 PERH--SVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMS 468
           PERH  +V     YKL+PFG+GRRACPG  MAQRVVGL LASLIQ FEW+++ + L+DM 
Sbjct: 421 PERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMR 480

BLAST of MELO3C019216 vs. ExPASy TrEMBL
Match: A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 7.0e-177
Identity = 328/489 (67.08%), Postives = 383/489 (78.32%), Query Frame = 0

Query: 6   LLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGH 65
           LL   LSL SL + + FL +P+R NLPP+P F LP IGHLHL+KHPVH+T   LS  YGH
Sbjct: 85  LLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNYGH 144

Query: 66  VFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKHWR 125
           VFSL++GSRLVV+VSSPSA+QECFTKNDI+LANRP L SGK+LAYN+TT+ VS YG+HWR
Sbjct: 145 VFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEHWR 204

Query: 126 NLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLDLTFNI 185
           NLRRISTLEIFST+RLN F   RE+EVK LLRKL G+     EFR VEL+ MLLD TFNI
Sbjct: 205 NLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSL--QEFRAVELDSMLLDHTFNI 264

Query: 186 VMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL---------------------WL----YE 245
           V+RM+ GKK+  E     L DEG  K+F+E+                     W+    YE
Sbjct: 265 VVRMIAGKKYYGED----LRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYE 324

Query: 246 KDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIIL 305
           K IMKIG ++DE+LQ LVDEIRN++D GNTMI HLL LQ T PEY+SDQIIKGL Q ++L
Sbjct: 325 KRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLL 384

Query: 306 AGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETL 365
           AGIDT+ I+LEWALSQLLNNPEVLKKA+ EID  IG  RLVSE DL SLSYLQG+I+ETL
Sbjct: 385 AGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETL 444

Query: 366 RLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVI 425
           RL+P APLLVPHCA  E+CKI+ Y+IPRDTI+LINAWAIHRDS +WEDAT FKPERH+  
Sbjct: 445 RLNPAAPLLVPHCA-SEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANT 504

Query: 426 ES--ELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTM 468
               + YKL+PFG+GRRACPG GMAQRVVGL LASLIQ FEWERV+ SL+DM+EG+GLTM
Sbjct: 505 MGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTM 564

BLAST of MELO3C019216 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 469.9 bits (1208), Expect = 2.3e-132
Identity = 251/490 (51.22%), Postives = 333/490 (67.96%), Query Frame = 0

Query: 6   LLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGH 65
           +LY++ SLI L++ + FL KP + NLPP+P   LP IGHL LLK P+H+T ++ S+   H
Sbjct: 9   VLYSIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 68

Query: 66  -----VFSLKFGSRLVVLVSSPS-AIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSS 125
                V SL+ GSRLV +VSS   A +ECF KND++LANRP +  GK++ YNNT M+ + 
Sbjct: 69  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 128

Query: 126 YGKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLL 185
           YG HWRNLRR+ T+EIFST RLN F   R +EV+RL+ +L  +     +  VVEL+PML+
Sbjct: 129 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRL--SRLAGTKKTVVELKPMLM 188

Query: 186 DLTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELWL--------------------- 245
           DLTFN +MRM+ GK++  E+      DE   KR ++L                       
Sbjct: 189 DLTFNNIMRMMTGKRYYGEETT----DEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF 248

Query: 246 --YEKDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQ 305
             YE  + K+G +TD+ LQGL+D+ R +++ G TMI HLL LQK+D EYY+DQIIKG++ 
Sbjct: 249 SSYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIIL 308

Query: 306 DIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGII 365
            +++AG +TS ++LEWALS LLN+P+V+ KA++EID  +G  RL+ E DL  L YL+ I+
Sbjct: 309 IMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIV 368

Query: 366 SETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPER 425
            ETLRLHP  PLLVPH A  E+CKI  Y++PR T +L+NAWAIHRD   W+D   FKPER
Sbjct: 369 LETLRLHPATPLLVPHMA-SEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPER 428

Query: 426 HSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGL 467
               E E  KL+ FG+GRRACPG+G+AQR+VGLAL SLIQ FEWERV +  +DM EG G 
Sbjct: 429 FEK-EEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGN 488

BLAST of MELO3C019216 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 456.8 bits (1174), Expect = 2.0e-128
Identity = 245/486 (50.41%), Postives = 335/486 (68.93%), Query Frame = 0

Query: 6   LLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGH 65
           +L+ +LSLI L+       KP   NLPP+P + LP IGHL LLK P+H+TF +LSQ   +
Sbjct: 10  ILFVVLSLIYLI--GKLKRKP---NLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNN 69

Query: 66  --VFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKH 125
             +FSL+ G+RLV + SS S  +ECFTKND++LANRP+    K++AY+ TTM+ +SYG H
Sbjct: 70  APIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDH 129

Query: 126 WRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLDLTF 185
           WRNLRRI ++EIFS  RLNSF   R++E++RL+ +L  N+    EF  V+++ ML DLTF
Sbjct: 130 WRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNF--SQEFVKVDMKSMLSDLTF 189

Query: 186 NIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELWL-----------------------YE 245
           N ++RMV GK++      + +ED+   KR ++L                         YE
Sbjct: 190 NNILRMVAGKRY----YGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVSDYE 249

Query: 246 KDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIIL 305
             + K+  + DE LQGLVDE R  +++GNTMI HLL LQ++ P+Y++D+IIKG +  +IL
Sbjct: 250 TRVKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALIL 309

Query: 306 AGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETL 365
           AG DTS ++LEWALS +LN+P+VL KA++EID  IG  RL+ E D+ +L YLQ I+SETL
Sbjct: 310 AGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETL 369

Query: 366 RLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVI 425
           RL+P AP+L+PH A  E+CK+  Y++PR TI+L N WAIHRD  LW+D   FKPER    
Sbjct: 370 RLYPAAPMLLPHVA-SEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK- 429

Query: 426 ESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPK 467
           E E  KL+PFG+GRRACPG+G+A R++ L L SLIQ  EWE++ +  +DMSEGKG+TMPK
Sbjct: 430 EGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPK 481

BLAST of MELO3C019216 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 452.6 bits (1163), Expect = 3.8e-127
Identity = 242/465 (52.04%), Postives = 320/465 (68.82%), Query Frame = 0

Query: 28  RLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGH--VFSLKFGSRLVVLVSSPSAI 87
           +LNLPP+P + LP IGHL LLK P+H+ F ++SQ  G   + SL+ G+RL+ +VSS S  
Sbjct: 27  KLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIISLRLGNRLLFVVSSHSIA 86

Query: 88  QECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKHWRNLRRISTLEIFSTTRLNSFS 147
           +ECFTKND+ILANR +  S K+++Y N+T+V +SY +HWRNLRRI  LEIFS  RLNSFS
Sbjct: 87  EECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLRRIGALEIFSAHRLNSFS 146

Query: 148 RTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLDLTFNIVMRMVGGKKFCEEKNNNVLE 207
             R +E++RL+ +L  N      F  VE++ M  DLTFN ++RM+ GK +  +      E
Sbjct: 147 SIRRDEIRRLIGRLLRNSSY--GFTKVEMKSMFSDLTFNNIIRMLAGKCYYGDGK----E 206

Query: 208 DEGYCKRFKEL---------------------WL--YEKDIMKIGRKTDEVLQGLVDEIR 267
           D+   KR + L                     W+   E  I K+  + DE LQGLVDE R
Sbjct: 207 DDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYSETRIKKLAGRLDEFLQGLVDEKR 266

Query: 268 -NEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGIDTSTISLEWALSQLLNNP 327
             +E + NTM+ HLL LQ+T PEYY D+IIKG +  +I  G DT+ ++LEWALS LLNNP
Sbjct: 267 EGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNP 326

Query: 328 EVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLHPPAPLLVPHCALFEECKI 387
           EVL KA++EID +IG  RL+ E D+P+L YLQ I+SETLRL+P AP+L+PH A  ++CK+
Sbjct: 327 EVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAPMLLPHVA-SKDCKV 386

Query: 388 KRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIESELYKLIPFGVGRRACPGAG 447
             Y++PR T++L NAWAIHRD  LW+D T FKPER    E E  KL+PFG+GRRACPG+G
Sbjct: 387 GGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEK-EGEAKKLMPFGLGRRACPGSG 446

Query: 448 MAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKAQPLIA 467
           +AQR+V L+L SLIQ FEWER+ +  +DM+EG GLTMPKA+PL A
Sbjct: 447 LAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEA 483

BLAST of MELO3C019216 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 447.6 bits (1150), Expect = 1.2e-125
Identity = 239/493 (48.48%), Postives = 329/493 (66.73%), Query Frame = 0

Query: 1   MDFDHLLYTLLSLISLLLGYSFLF--KPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQT 60
           MD   +L  LLS + L +    L      + NLPP+P + LP IGHLHLLK PVH+TF +
Sbjct: 1   MDLTQIL--LLSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHS 60

Query: 61  LSQKYGH--VFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMV 120
           +S+  G+  +F L+ G+RLV ++SS S  +ECFTKND++LANRP +   K++ YN T M+
Sbjct: 61  ISKSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMI 120

Query: 121 VSSYGKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEP 180
            +SYG HWRNLRRI+ +EIFS+ R+++FS  R++E++RL+  L  +      F  VEL+ 
Sbjct: 121 AASYGDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHL--SRDSLHGFVEVELKS 180

Query: 181 MLLDLTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKEL-------------------- 240
           +L +L FN ++ MV GK++         ED    K  +EL                    
Sbjct: 181 LLTNLAFNNIIMMVAGKRY----YGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSI 240

Query: 241 -WL--YEKDIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKG 300
            W+  +E     +G + D VLQ LVDE R E+++G T+I HLL  Q+T+PEYY+D IIKG
Sbjct: 241 NWVTNFENQTKILGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKG 300

Query: 301 LVQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQ 360
           ++  ++LAG DTS+++LEWA+S LLN+PE+L+KA+ EID  IG  RLV E D+ +L YLQ
Sbjct: 301 IILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQ 360

Query: 361 GIISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFK 420
            I+SETLRL+P  PLL+PH +  +ECK+  Y++PR T++L N WA+HRD GLWE+   FK
Sbjct: 361 NIVSETLRLYPAVPLLLPHFS-SDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFK 420

Query: 421 PERHSVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEG 467
           PER    E E  KL+PFG+GRRACPGA + +R+V LAL  LIQSFEWERV   L+DM+EG
Sbjct: 421 PERFEK-EGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEG 480

BLAST of MELO3C019216 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 446.4 bits (1147), Expect = 2.8e-125
Identity = 246/486 (50.62%), Postives = 327/486 (67.28%), Query Frame = 0

Query: 7   LYTLLSL--ISLLLGYSFLF--KPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQK 66
           +Y +LSL    + L    LF  +P +LNLPP+P    P IGHLHLLK P+H+ F +LS+ 
Sbjct: 11  IYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSES 70

Query: 67  YGH--VFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 126
             +  +FSL  GSRLV +VSS +  +ECFTKND++LANRP    GK++ YN+TTMV ++Y
Sbjct: 71  LNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAY 130

Query: 127 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLD 186
           G  WRNLRRI T+EIFS+ RLNSF   R++E++RL+  L  N   +  F  VE++P+ + 
Sbjct: 131 GDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKN--SQHGFVKVEMKPLFMC 190

Query: 187 LTFNIVMRMVGGKKFCEEKNNNVLEDE-------------------GYCKRFKELWLYEK 246
           LT N ++RMV GK+F  +   N  E +                    Y    + +  YEK
Sbjct: 191 LTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYVTNYEK 250

Query: 247 DIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILA 306
            + K+  + DE LQ LV+E R E+ +GNTMI HLL LQ+T P+YY+D IIKG++  +ILA
Sbjct: 251 HVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVMILA 310

Query: 307 GIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLR 366
           G DTS  +LEWA+S LLN+PEVL+KAK EID  IG  RLV E D+  L YLQ I+SETLR
Sbjct: 311 GTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLR 370

Query: 367 LHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERHSVIE 426
           L+P AP+L+PH A  E+C +  Y++PR TI+L+NAWAIHRD  LWE+  +FKPER    +
Sbjct: 371 LYPVAPMLLPHLA-SEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEK-K 430

Query: 427 SELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEG-KGLTMPK 467
            E  KL+PFG+GRR+CPG+G+AQR+V LAL SL+Q FEWERV++  +DM E  KG TM K
Sbjct: 431 GEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRK 490

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYJ98343.12.0e-26398.93isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa][more]
XP_008455822.25.0e-26294.31PREDICTED: LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cucumis melo][more]
KAA0032538.11.8e-22781.98isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa][more]
XP_004151924.29.0e-21177.22cytochrome P450 81Q32 [Cucumis sativus] >KGN63284.1 hypothetical protein Csa_022... [more]
XP_038902403.12.9e-20173.39cytochrome P450 81Q32-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FG653.3e-13151.22Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1[more]
W8JMU76.2e-13050.31Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
Q6WNQ92.0e-12851.43Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... [more]
Q6WNQ83.5e-12551.23Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
P931477.8e-12549.59Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3BGH39.9e-26498.93Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3C1S22.4e-26294.31LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=L... [more]
A0A5A7SPU08.7e-22881.98Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A0A0LQI54.3e-21177.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1[more]
A0A6J1D5S67.0e-17767.08isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE... [more]
Match NameE-valueIdentityDescription
AT5G36220.12.3e-13251.22cytochrome p450 81d1 [more]
AT4G37370.12.0e-12850.41cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37360.13.8e-12752.04cytochrome P450, family 81, subfamily D, polypeptide 2 [more]
AT4G37320.11.2e-12548.48cytochrome P450, family 81, subfamily D, polypeptide 5 [more]
AT3G28740.12.8e-12550.62Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 333..351
score: 35.67
coord: 290..316
score: 41.63
coord: 84..105
score: 21.19
coord: 270..287
score: 30.99
coord: 375..399
score: 29.85
coord: 417..440
score: 33.88
coord: 60..79
score: 39.83
coord: 407..417
score: 55.07
IPR001128Cytochrome P450PRINTSPR00385P450coord: 334..345
score: 40.74
coord: 408..417
score: 58.53
coord: 281..298
score: 36.77
coord: 417..428
score: 29.12
IPR001128Cytochrome P450PFAMPF00067p450coord: 32..461
e-value: 9.0E-82
score: 275.2
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 24..465
e-value: 1.0E-109
score: 369.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 32..465
NoneNo IPR availablePANTHERPTHR24298:SF836CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATEDcoord: 218..466
coord: 7..216
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 218..466
coord: 7..216
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 410..419

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C019216.1MELO3C019216.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen