MELO3C018593 (gene) Melon (DHL92) v4

Overview
NameMELO3C018593
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionCystatin domain containing protein
Locationchr01: 1365172 .. 1365843 (-)
RNA-Seq ExpressionMELO3C018593
SyntenyMELO3C018593
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCATCACAAACTAAACCTTTTGTCCATAATACACACACCAATAACTAAATCAAGTTAGATATCACAATGATTTCTACCGTGCTTGCCAACATCGGTCAACATGATCCCATCCTCCATGTGACGTCAAAGGATACGAAGGGTGATCATCAATCCGAATGGATATCGATTCGCGCTATCGAAGACCCTTACGTGCAAGAAGTCGCGAAATTCGCCGTGAGAGAGTTCGAGAAGGAAAAAATGGCAAGATCTCAATACAAACGGTTGCTAAGTTGTTGGATTAAAGAGGAGATGGGAGGTATAAAATATCGTCTTATCCTCGAGGTAATTAAAGAAGAAAAAATTGAGGGTAAATATGAGGCTATTGTATGGGAGGCTTCTGGCCGTTTGATTATAAGGAAGCTTAAGCTTATATCTTTCATGCCTTGTATGCATGGGGTTGCATGACATTTCCATCCCCTCTTTGTATAATTCAATCTACAAAGCTTATGGTGTGAAATGCCATTGGTAAAATAAACAAAAAAAGGAAAAAGAAGTATATATATACTTTCTATGTAAAATTCTCTTCTCTATGTTATTCAAAAACTATGGATCTTTGTGTTCAATCAAACAATTAATCATCCAATCAACTTAGTTTTTAGATTTATATTTTGAATTTATTCATAATGTGAAAA

mRNA sequence

ATCATCACAAACTAAACCTTTTGTCCATAATACACACACCAATAACTAAATCAAGTTAGATATCACAATGATTTCTACCGTGCTTGCCAACATCGGTCAACATGATCCCATCCTCCATGTGACGTCAAAGGATACGAAGGGTGATCATCAATCCGAATGGATATCGATTCGCGCTATCGAAGACCCTTACGTGCAAGAAGTCGCGAAATTCGCCGTGAGAGAGTTCGAGAAGGAAAAAATGGCAAGATCTCAATACAAACGGTTGCTAAGTTGTTGGATTAAAGAGGAGATGGGAGGTATAAAATATCGTCTTATCCTCGAGGTAATTAAAGAAGAAAAAATTGAGGGTAAATATGAGGCTATTGTATGGGAGGCTTCTGGCCGTTTGATTATAAGGAAGCTTAAGCTTATATCTTTCATGCCTTGTATGCATGGGGTTGCATGACATTTCCATCCCCTCTTTGTATAATTCAATCTACAAAGCTTATGGTGTGAAATGCCATTGGTAAAATAAACAAAAAAAGGAAAAAGAAGTATATATATACTTTCTATGTAAAATTCTCTTCTCTATGTTATTCAAAAACTATGGATCTTTGTGTTCAATCAAACAATTAATCATCCAATCAACTTAGTTTTTAGATTTATATTTTGAATTTATTCATAATGTGAAAA

Coding sequence (CDS)

ATGATTTCTACCGTGCTTGCCAACATCGGTCAACATGATCCCATCCTCCATGTGACGTCAAAGGATACGAAGGGTGATCATCAATCCGAATGGATATCGATTCGCGCTATCGAAGACCCTTACGTGCAAGAAGTCGCGAAATTCGCCGTGAGAGAGTTCGAGAAGGAAAAAATGGCAAGATCTCAATACAAACGGTTGCTAAGTTGTTGGATTAAAGAGGAGATGGGAGGTATAAAATATCGTCTTATCCTCGAGGTAATTAAAGAAGAAAAAATTGAGGGTAAATATGAGGCTATTGTATGGGAGGCTTCTGGCCGTTTGATTATAAGGAAGCTTAAGCTTATATCTTTCATGCCTTGTATGCATGGGGTTGCATGA

Protein sequence

MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMARSQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPCMHGVA
Homology
BLAST of MELO3C018593 vs. NCBI nr
Match: KAA0031436.1 (Cystatin domain containing protein [Cucumis melo var. makuwa] >TYK06887.1 Cystatin domain containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 251.5 bits (641), Expect = 3.7e-63
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0

Query: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60
           MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR
Sbjct: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60

Query: 61  SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120
           SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC
Sbjct: 61  SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120

Query: 121 MHGVA 126
           MHGVA
Sbjct: 121 MHGVA 125

BLAST of MELO3C018593 vs. NCBI nr
Match: KGN43767.1 (hypothetical protein Csa_017208 [Cucumis sativus])

HSP 1 Score: 239.6 bits (610), Expect = 1.5e-59
Identity = 116/125 (92.80%), Postives = 122/125 (97.60%), Query Frame = 0

Query: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60
           MIST+LAN+GQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFA+REFEKEKMAR
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120
            QYKRLLSCWIKEEMGGIKYRL+LEVIKEEK+EGKYEA+VWEASGRLIIRKLKLISFMP 
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 MHGVA 126
           MHG A
Sbjct: 121 MHGAA 125

BLAST of MELO3C018593 vs. NCBI nr
Match: KAG6571859.1 (hypothetical protein SDJN03_28587, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011544.1 hypothetical protein SDJN02_26450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 185.7 bits (470), Expect = 2.5e-43
Identity = 97/120 (80.83%), Postives = 101/120 (84.17%), Query Frame = 0

Query: 5   VLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMARSQYK 64
           +LANIGQHDPILHVT KD KGDH SEWISIRAIEDPYVQEVAKF V E  KEK A  QYK
Sbjct: 1   MLANIGQHDPILHVT-KDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYK 60

Query: 65  RLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPCMHGV 124
            LL CW+KEEMGGIKYRLILEV K    EGKYEAIVWEASGRLIIRKLKL+SF+ C HGV
Sbjct: 61  GLLRCWVKEEMGGIKYRLILEV-KGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHGV 118

BLAST of MELO3C018593 vs. NCBI nr
Match: KAG6588760.1 (hypothetical protein SDJN03_17325, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 182.6 bits (462), Expect = 2.1e-42
Identity = 93/122 (76.23%), Postives = 103/122 (84.43%), Query Frame = 0

Query: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60
           MIST+L NIGQHDPILHVT KD KGD +SEWISIR IEDPYVQEVAKFAV EF  +KM  
Sbjct: 1   MISTLLTNIGQHDPILHVT-KDVKGDPRSEWISIRTIEDPYVQEVAKFAVIEFNVDKMTT 60

Query: 61  SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEG--KYEAIVWEASGRLIIRKLKLISFM 120
            QYKR+L CWIKEEMGGIKYRL+LEV +E   +   KYEA+VWEASGRLIIRKLKL+SF+
Sbjct: 61  FQYKRMLRCWIKEEMGGIKYRLVLEVKQEAPTQKVCKYEAVVWEASGRLIIRKLKLVSFV 120

BLAST of MELO3C018593 vs. NCBI nr
Match: XP_022973098.1 (cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine proteinase inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 60.8 bits (146), Expect = 9.4e-06
Identity = 32/89 (35.96%), Postives = 55/89 (61.80%), Query Frame = 0

Query: 31  WISIRAIEDPYVQEVAKFAVREFEKEKMARSQYKRLLSCWIKEEMGGIKYRLILEVIKEE 90
           W  I+ ++DP++QE+ KFAV E+ K+     ++K ++     + + G+ YRL++E  K E
Sbjct: 31  WEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQVVSGMNYRLVIEAKKGE 90

Query: 91  KIEGKYEAIVWEASGRLIIRKLKLISFMP 120
            I GKY+A+VWE + +     ++L SF P
Sbjct: 91  SI-GKYQALVWEKAWQ---HFMELTSFKP 114

BLAST of MELO3C018593 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 8.2e-05
Identity = 30/83 (36.14%), Postives = 46/83 (55.42%), Query Frame = 0

Query: 37  IEDPYVQEVAKFAVREFEKEKMARSQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKY 96
           I DP+VQE+ ++AV E ++   A       ++   K+ + G+ YRL LE       +G+Y
Sbjct: 35  INDPHVQELGRWAVAEEDRGVAAGGLTFERVTDGEKQVVAGVNYRLTLEASSSGAKDGRY 94

Query: 97  EAIVWEASGRLIIRKLKLISFMP 120
           EA+V+E   R   R  KL+SF P
Sbjct: 95  EAVVYEQDPRSNAR--KLVSFEP 115

BLAST of MELO3C018593 vs. ExPASy TrEMBL
Match: A0A5A7SN35 (Cystatin domain containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001920 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.8e-63
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0

Query: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60
           MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR
Sbjct: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60

Query: 61  SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120
           SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC
Sbjct: 61  SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120

Query: 121 MHGVA 126
           MHGVA
Sbjct: 121 MHGVA 125

BLAST of MELO3C018593 vs. ExPASy TrEMBL
Match: A0A0A0K644 (Cystatin domain containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G066240 PE=4 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 7.1e-60
Identity = 116/125 (92.80%), Postives = 122/125 (97.60%), Query Frame = 0

Query: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60
           MIST+LAN+GQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFA+REFEKEKMAR
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120
            QYKRLLSCWIKEEMGGIKYRL+LEVIKEEK+EGKYEA+VWEASGRLIIRKLKLISFMP 
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 MHGVA 126
           MHG A
Sbjct: 121 MHGAA 125

BLAST of MELO3C018593 vs. ExPASy TrEMBL
Match: A0A2P2NS36 (Cysteine proteinase inhibitor OS=Rhizophora mucronata OX=61149 PE=4 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.0e-06
Identity = 37/89 (41.57%), Postives = 53/89 (59.55%), Query Frame = 0

Query: 31  WISIRAIEDPYVQEVAKFAVREFEKEKMARSQYKRLLSCWIKEEMGGIKYRLILEVIKEE 90
           W  I  + DP+VQE+A +AVRE+     A  + +RL+    K+ + GIKYRL+L  +KE 
Sbjct: 32  WTPITNLSDPHVQEIASYAVREYNHRSNANLKLQRLVK-GEKQVVSGIKYRLVL-AVKEG 91

Query: 91  KIEGKYEAIVWEASGRLIIRKLKLISFMP 120
             + KY+A+VWE +        KLISF P
Sbjct: 92  GSDKKYQAVVWEKAWE---HFRKLISFKP 115

BLAST of MELO3C018593 vs. ExPASy TrEMBL
Match: A0A6J1IC35 (cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 4.5e-06
Identity = 32/89 (35.96%), Postives = 55/89 (61.80%), Query Frame = 0

Query: 31  WISIRAIEDPYVQEVAKFAVREFEKEKMARSQYKRLLSCWIKEEMGGIKYRLILEVIKEE 90
           W  I+ ++DP++QE+ KFAV E+ K+     ++K ++     + + G+ YRL++E  K E
Sbjct: 31  WEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQVVSGMNYRLVIEAKKGE 90

Query: 91  KIEGKYEAIVWEASGRLIIRKLKLISFMP 120
            I GKY+A+VWE + +     ++L SF P
Sbjct: 91  SI-GKYQALVWEKAWQ---HFMELTSFKP 114

BLAST of MELO3C018593 vs. ExPASy TrEMBL
Match: A0A6J1EKC1 (cysteine proteinase inhibitor 5-like OS=Cucurbita moschata OX=3662 GN=LOC111434113 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 5.9e-06
Identity = 31/89 (34.83%), Postives = 55/89 (61.80%), Query Frame = 0

Query: 31  WISIRAIEDPYVQEVAKFAVREFEKEKMARSQYKRLLSCWIKEEMGGIKYRLILEVIKEE 90
           W  I+ ++DP++QE+ KFAV E+ K+     ++K ++     + + G+ YRL++E  K E
Sbjct: 31  WEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQVVSGMNYRLVIEAKKGE 90

Query: 91  KIEGKYEAIVWEASGRLIIRKLKLISFMP 120
            I GKY+A++WE + +     ++L SF P
Sbjct: 91  SI-GKYQALIWEKAWQ---HFMELTSFKP 114

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0031436.13.7e-63100.00Cystatin domain containing protein [Cucumis melo var. makuwa] >TYK06887.1 Cystat... [more]
KGN43767.11.5e-5992.80hypothetical protein Csa_017208 [Cucumis sativus][more]
KAG6571859.12.5e-4380.83hypothetical protein SDJN03_28587, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6588760.12.1e-4276.23hypothetical protein SDJN03_17325, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022973098.19.4e-0635.96cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine p... [more]
Match NameE-valueIdentityDescription
Q10Q478.2e-0536.14Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=3994... [more]
Match NameE-valueIdentityDescription
A0A5A7SN351.8e-63100.00Cystatin domain containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A0A0K6447.1e-6092.80Cystatin domain containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G066240 PE... [more]
A0A2P2NS362.0e-0641.57Cysteine proteinase inhibitor OS=Rhizophora mucronata OX=61149 PE=4 SV=1[more]
A0A6J1IC354.5e-0635.96cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628... [more]
A0A6J1EKC15.9e-0634.83cysteine proteinase inhibitor 5-like OS=Cucurbita moschata OX=3662 GN=LOC1114341... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 34..105
e-value: 6.9E-12
score: 45.6
NoneNo IPR availableGENE3D3.10.450.10coord: 25..120
e-value: 3.7E-15
score: 57.9
NoneNo IPR availablePANTHERPTHR47364CYSTEINE PROTEINASE INHIBITOR 5coord: 19..119
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 31..120

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C018593.1MELO3C018593.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity