MELO3C017296.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C017296.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Descriptionprotein ASPARTIC PROTEASE IN GUARD CELL 1
Locationchr02: 22482307 .. 22483974 (-)
RNA-Seq ExpressionMELO3C017296.jh1
SyntenyMELO3C017296.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTATAAGTTCATTGCAACAATATGATGAACACTTCACTTTCCTATGCTCTTCTCTTCCTGACAATCTTCACTTTCCTTCAATTCCCATCAATTCTCTCTCGCAAACTAACAGCACAATCTCCTTATTCCACTACCACCTTTGATGTCTCGGCCTCCATAAACCAAGCCCTAAATGCCCTTTCCATCAAACCCAAACCCTTTCAAACTCACTCTTACCATTCAAATTCACCTTTATCTCTCTCATTGCACCCTAGATTGACCGTTCATAACCCTTCTTACAAGGACTATGGTACTCTTGTTAGGGCCCGACTCGCTCGTCATGCCACCCGAGTTCAATCCCTTAATAGAAAGCTTGAGCTCTCTTTAAATGGAGCTAAACAATTTGGTAAAAGAATTAATGGATCCGCGTCTACAAATTCACTTACTGCTCCGGTTACTTCAGGGGCGAGTCATGGGGATGGTGAATATTTTGCCCGGATTGGGGTCGGTCAGCCTGTGCAATCGTTTTTTTTGGTGCCTGATACTGGCAGTGATGTTACGTGGCTTCAATGTAAGCCTTGTGCTAATGAGAATGCTTGTTTTAAACAACTTGACCCGATATTTGACCCAAAATCGTCGTCTTCTTACAGTTCGTTGTCTTGTAATTCAGAACAATGTCAATTGTTGGATGAAGCCGGTTGTAGTTCCAATTCGTGTATCTACGAAGTTGAATATGGCGATGGATCATTCACAATCGGTGAACTCGCCACCGAAACGTTATCATTTGGAAATTCTAATTCCATCCCTAATCTCCCGATTGGTTGTGGCCATGACAACGAAGGCCTCTTTGATGCTGCTGCTGGTTTAATTGGCCTCGGTGGTGGGGCCATTTCCCTTTCTTCCCAATTACAAGCGTCGTCATTTTCATATTGTTTGGTCGACCTAGACTCAGACTCATCCTCCACTCTTGACTTCAACGCAGACCAACCAAGCGACTCACTCACATCCCCACTCGTGAAAAATAATCGATTCCCCTCGTTTCGGTACGTGAAAGTAATTGGAATGAGTGTCGGCGGGAAGCGTTTACCGATTTCCTCGTCGAGATTTGAAATCGACGAATCAGGATCAGGGGGAATAATCGTGGATTCTGGGACGACTATAACTCAACTACCAAGTGATGTGTACGACGTGTTAAGAGACGCGTTCGTGGGGCTAACGACGAACCTCCCAACGGCACCAGGGGTATCACCTTTTGATACTTGTTATGACTTATCAAGTCAATCGAGTGTGGAGGTCCCGATCATAGCATTCATTTTGCCAGGAGGAAAATCTCTAAAACTACCAGCAAAAAATTGTTTGATCCAGGTGGACTCAGCTGGAACTTTCTGCTTGGCGTTTCTTCCAGGGACATTCCCGCTTTCTATTATTGGAAACGTCCAACAACAAGGAATACGTGTGAGTTATGACTTGGACAACTCTATCGTTGGATTTGCGACTAATAAATGTTAAAACTCAGCTTGACACGTGGGTGAAATTTGGGTTTATTACATATATTATTCTAAGCCAATAAAAATACTATTGCTTATCGTACATTGTGTAAAAAGAAAAAGTTTATTTAATAGTGGGTTGAATTAATCAAATGATGTCAATGTAATTTCTTCATATTAATGATACTGTTTGATTGCATG

mRNA sequence

TTTATAAGTTCATTGCAACAATATGATGAACACTTCACTTTCCTATGCTCTTCTCTTCCTGACAATCTTCACTTTCCTTCAATTCCCATCAATTCTCTCTCGCAAACTAACAGCACAATCTCCTTATTCCACTACCACCTTTGATGTCTCGGCCTCCATAAACCAAGCCCTAAATGCCCTTTCCATCAAACCCAAACCCTTTCAAACTCACTCTTACCATTCAAATTCACCTTTATCTCTCTCATTGCACCCTAGATTGACCGTTCATAACCCTTCTTACAAGGACTATGGTACTCTTGTTAGGGCCCGACTCGCTCGTCATGCCACCCGAGTTCAATCCCTTAATAGAAAGCTTGAGCTCTCTTTAAATGGAGCTAAACAATTTGGTAAAAGAATTAATGGATCCGCGTCTACAAATTCACTTACTGCTCCGGTTACTTCAGGGGCGAGTCATGGGGATGGTGAATATTTTGCCCGGATTGGGGTCGGTCAGCCTGTGCAATCGTTTTTTTTGGTGCCTGATACTGGCAGTGATGTTACGTGGCTTCAATGTAAGCCTTGTGCTAATGAGAATGCTTGTTTTAAACAACTTGACCCGATATTTGACCCAAAATCGTCGTCTTCTTACAGTTCGTTGTCTTGTAATTCAGAACAATGTCAATTGTTGGATGAAGCCGGTTGTAGTTCCAATTCGTGTATCTACGAAGTTGAATATGGCGATGGATCATTCACAATCGGTGAACTCGCCACCGAAACGTTATCATTTGGAAATTCTAATTCCATCCCTAATCTCCCGATTGGTTGTGGCCATGACAACGAAGGCCTCTTTGATGCTGCTGCTGGTTTAATTGGCCTCGGTGGTGGGGCCATTTCCCTTTCTTCCCAATTACAAGCGTCGTCATTTTCATATTGTTTGGTCGACCTAGACTCAGACTCATCCTCCACTCTTGACTTCAACGCAGACCAACCAAGCGACTCACTCACATCCCCACTCGTGAAAAATAATCGATTCCCCTCGTTTCGGTACGTGAAAGTAATTGGAATGAGTGTCGGCGGGAAGCGTTTACCGATTTCCTCGTCGAGATTTGAAATCGACGAATCAGGATCAGGGGGAATAATCGTGGATTCTGGGACGACTATAACTCAACTACCAAGTGATGTGTACGACGTGTTAAGAGACGCGTTCGTGGGGCTAACGACGAACCTCCCAACGGCACCAGGGGTATCACCTTTTGATACTTGTTATGACTTATCAAGTCAATCGAGTGTGGAGGTCCCGATCATAGCATTCATTTTGCCAGGAGGAAAATCTCTAAAACTACCAGCAAAAAATTGTTTGATCCAGGTGGACTCAGCTGGAACTTTCTGCTTGGCGTTTCTTCCAGGGACATTCCCGCTTTCTATTATTGGAAACGTCCAACAACAAGGAATACGTGTGAGTTATGACTTGGACAACTCTATCGTTGGATTTGCGACTAATAAATGTTAAAACTCAGCTTGACACGTGGGTGAAATTTGGGTTTATTACATATATTATTCTAAGCCAATAAAAATACTATTGCTTATCGTACATTGTGTAAAAAGAAAAAGTTTATTTAATAGTGGGTTGAATTAATCAAATGATGTCAATGTAATTTCTTCATATTAATGATACTGTTTGATTGCATG

Coding sequence (CDS)

ATGATGAACACTTCACTTTCCTATGCTCTTCTCTTCCTGACAATCTTCACTTTCCTTCAATTCCCATCAATTCTCTCTCGCAAACTAACAGCACAATCTCCTTATTCCACTACCACCTTTGATGTCTCGGCCTCCATAAACCAAGCCCTAAATGCCCTTTCCATCAAACCCAAACCCTTTCAAACTCACTCTTACCATTCAAATTCACCTTTATCTCTCTCATTGCACCCTAGATTGACCGTTCATAACCCTTCTTACAAGGACTATGGTACTCTTGTTAGGGCCCGACTCGCTCGTCATGCCACCCGAGTTCAATCCCTTAATAGAAAGCTTGAGCTCTCTTTAAATGGAGCTAAACAATTTGGTAAAAGAATTAATGGATCCGCGTCTACAAATTCACTTACTGCTCCGGTTACTTCAGGGGCGAGTCATGGGGATGGTGAATATTTTGCCCGGATTGGGGTCGGTCAGCCTGTGCAATCGTTTTTTTTGGTGCCTGATACTGGCAGTGATGTTACGTGGCTTCAATGTAAGCCTTGTGCTAATGAGAATGCTTGTTTTAAACAACTTGACCCGATATTTGACCCAAAATCGTCGTCTTCTTACAGTTCGTTGTCTTGTAATTCAGAACAATGTCAATTGTTGGATGAAGCCGGTTGTAGTTCCAATTCGTGTATCTACGAAGTTGAATATGGCGATGGATCATTCACAATCGGTGAACTCGCCACCGAAACGTTATCATTTGGAAATTCTAATTCCATCCCTAATCTCCCGATTGGTTGTGGCCATGACAACGAAGGCCTCTTTGATGCTGCTGCTGGTTTAATTGGCCTCGGTGGTGGGGCCATTTCCCTTTCTTCCCAATTACAAGCGTCGTCATTTTCATATTGTTTGGTCGACCTAGACTCAGACTCATCCTCCACTCTTGACTTCAACGCAGACCAACCAAGCGACTCACTCACATCCCCACTCGTGAAAAATAATCGATTCCCCTCGTTTCGGTACGTGAAAGTAATTGGAATGAGTGTCGGCGGGAAGCGTTTACCGATTTCCTCGTCGAGATTTGAAATCGACGAATCAGGATCAGGGGGAATAATCGTGGATTCTGGGACGACTATAACTCAACTACCAAGTGATGTGTACGACGTGTTAAGAGACGCGTTCGTGGGGCTAACGACGAACCTCCCAACGGCACCAGGGGTATCACCTTTTGATACTTGTTATGACTTATCAAGTCAATCGAGTGTGGAGGTCCCGATCATAGCATTCATTTTGCCAGGAGGAAAATCTCTAAAACTACCAGCAAAAAATTGTTTGATCCAGGTGGACTCAGCTGGAACTTTCTGCTTGGCGTTTCTTCCAGGGACATTCCCGCTTTCTATTATTGGAAACGTCCAACAACAAGGAATACGTGTGAGTTATGACTTGGACAACTCTATCGTTGGATTTGCGACTAATAAATGTTAA

Protein sequence

MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPFQTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQFGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPCANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGELATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVDLDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDESGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPIIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSIVGFATNKC
Homology
BLAST of MELO3C017296.jh1 vs. NCBI nr
Match: XP_008453383.1 (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis melo] >KAA0058062.1 protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis melo var. makuwa])

HSP 1 Score: 954 bits (2467), Expect = 0.0
Identity = 488/488 (100.00%), Postives = 488/488 (100.00%), Query Frame = 0

Query: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60
           MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF
Sbjct: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60

Query: 61  QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ 120
           QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ
Sbjct: 61  QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ 120

Query: 121 FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 180
           FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC
Sbjct: 121 FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 180

Query: 181 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 240
           ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE
Sbjct: 181 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 240

Query: 241 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 300
           LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD
Sbjct: 241 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 300

Query: 301 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 360
           LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES
Sbjct: 301 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 360

Query: 361 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 420
           GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI
Sbjct: 361 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 420

Query: 421 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 480
           IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI
Sbjct: 421 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 480

Query: 481 VGFATNKC 488
           VGFATNKC
Sbjct: 481 VGFATNKC 488

BLAST of MELO3C017296.jh1 vs. NCBI nr
Match: TYK28408.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis melo var. makuwa])

HSP 1 Score: 950 bits (2456), Expect = 0.0
Identity = 486/488 (99.59%), Postives = 487/488 (99.80%), Query Frame = 0

Query: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60
           MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF
Sbjct: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60

Query: 61  QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ 120
           QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ
Sbjct: 61  QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ 120

Query: 121 FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 180
           FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC
Sbjct: 121 FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 180

Query: 181 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 240
           ANENACFKQLDPIFDPK SSSYSSLSC+SEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE
Sbjct: 181 ANENACFKQLDPIFDPKLSSSYSSLSCDSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 240

Query: 241 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 300
           LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD
Sbjct: 241 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 300

Query: 301 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 360
           LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES
Sbjct: 301 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 360

Query: 361 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 420
           GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI
Sbjct: 361 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 420

Query: 421 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 480
           IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI
Sbjct: 421 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 480

Query: 481 VGFATNKC 488
           VGFATNKC
Sbjct: 481 VGFATNKC 488

BLAST of MELO3C017296.jh1 vs. NCBI nr
Match: KGN63815.2 (hypothetical protein Csa_013944 [Cucumis sativus])

HSP 1 Score: 831 bits (2147), Expect = 1.81e-301
Identity = 426/489 (87.12%), Postives = 451/489 (92.23%), Query Frame = 0

Query: 2   MNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPFQ 61
           MNTSLSYA LFLT FTFL FPSILSRKLT QSPYST TFDVSASINQALNALSIKPKPFQ
Sbjct: 1   MNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ 60

Query: 62  T-HS-YHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAK 121
           T HS YHS+SPLSLSLHPRLTVHNPSY+DYG+LVRARLAR A R QSLNRKLELSL G K
Sbjct: 61  TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARAQSLNRKLELSLKGGK 120

Query: 122 QFGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKP 181
           QFG+RINGS STNSLTAPVTSGAS G GEYFARIGVGQPVQS+F VPDTGSDV+WLQC+P
Sbjct: 121 QFGRRINGSDSTNSLTAPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQP 180

Query: 182 CANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIG 241
           C  EN C+KQ+ PIFDPKSSSSYS LSC+SEQC LLDEA C +NSCIYEVEYGDGSFT+G
Sbjct: 181 CDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDANSCIYEVEYGDGSFTVG 240

Query: 242 ELATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLV 301
           ELATET SF +SNSIPNLPIGCGHDNEGLF  AAGLIGLGGGAISLSSQL+A+SFSYCLV
Sbjct: 241 ELATETFSFRHSNSIPNLPIGCGHDNEGLFVGAAGLIGLGGGAISLSSQLEATSFSYCLV 300

Query: 302 DLDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDE 361
           DLDS+SSSTLDFNADQPSDSLTSPLVKN+RFP+FRYVKVIGMSVGGK LPISSS FEIDE
Sbjct: 301 DLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDE 360

Query: 362 SGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVP 421
           SGSGGIIVDSGTTIT++PSDVYDVLRDAFVGLT NLP APGVSPFDTCYDLSSQS+VEVP
Sbjct: 361 SGSGGIIVDSGTTITEIPSDVYDVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVP 420

Query: 422 IIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNS 481
            IAFILPG  SL+LPAKNCL QVDSAGTFCLAFLP TFPLSIIGNVQQQGIRVSYDL NS
Sbjct: 421 TIAFILPGENSLQLPAKNCLFQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANS 480

Query: 482 IVGFATNKC 488
           +VGF+T+KC
Sbjct: 481 LVGFSTDKC 489

BLAST of MELO3C017296.jh1 vs. NCBI nr
Match: XP_004138237.3 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis sativus])

HSP 1 Score: 831 bits (2147), Expect = 6.46e-301
Identity = 426/489 (87.12%), Postives = 451/489 (92.23%), Query Frame = 0

Query: 2   MNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPFQ 61
           MNTSLSYA LFLT FTFL FPSILSRKLT QSPYST TFDVSASINQALNALSIKPKPFQ
Sbjct: 35  MNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ 94

Query: 62  T-HS-YHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAK 121
           T HS YHS+SPLSLSLHPRLTVHNPSY+DYG+LVRARLAR A R QSLNRKLELSL G K
Sbjct: 95  TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARAQSLNRKLELSLKGGK 154

Query: 122 QFGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKP 181
           QFG+RINGS STNSLTAPVTSGAS G GEYFARIGVGQPVQS+F VPDTGSDV+WLQC+P
Sbjct: 155 QFGRRINGSDSTNSLTAPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQP 214

Query: 182 CANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIG 241
           C  EN C+KQ+ PIFDPKSSSSYS LSC+SEQC LLDEA C +NSCIYEVEYGDGSFT+G
Sbjct: 215 CDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDANSCIYEVEYGDGSFTVG 274

Query: 242 ELATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLV 301
           ELATET SF +SNSIPNLPIGCGHDNEGLF  AAGLIGLGGGAISLSSQL+A+SFSYCLV
Sbjct: 275 ELATETFSFRHSNSIPNLPIGCGHDNEGLFVGAAGLIGLGGGAISLSSQLEATSFSYCLV 334

Query: 302 DLDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDE 361
           DLDS+SSSTLDFNADQPSDSLTSPLVKN+RFP+FRYVKVIGMSVGGK LPISSS FEIDE
Sbjct: 335 DLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDE 394

Query: 362 SGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVP 421
           SGSGGIIVDSGTTIT++PSDVYDVLRDAFVGLT NLP APGVSPFDTCYDLSSQS+VEVP
Sbjct: 395 SGSGGIIVDSGTTITEIPSDVYDVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVP 454

Query: 422 IIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNS 481
            IAFILPG  SL+LPAKNCL QVDSAGTFCLAFLP TFPLSIIGNVQQQGIRVSYDL NS
Sbjct: 455 TIAFILPGENSLQLPAKNCLFQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANS 514

Query: 482 IVGFATNKC 488
           +VGF+T+KC
Sbjct: 515 LVGFSTDKC 523

BLAST of MELO3C017296.jh1 vs. NCBI nr
Match: XP_038878113.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida])

HSP 1 Score: 746 bits (1925), Expect = 1.06e-267
Identity = 386/488 (79.10%), Postives = 427/488 (87.50%), Query Frame = 0

Query: 2   MNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPFQ 61
           MN S+S A +FLTI T LQFPSI SRKLT QSPYST  FDVSAS  QA NALSIKPKPF+
Sbjct: 1   MNISISSAFIFLTILTSLQFPSIFSRKLT-QSPYSTAIFDVSASTKQAQNALSIKPKPFE 60

Query: 62  THSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQF 121
           THS+H NSPLSL LHPRLT++NPSYKDYG+LVRARLAR A RVQSLNR LELSLNG ++ 
Sbjct: 61  THSHHPNSPLSLPLHPRLTLYNPSYKDYGSLVRARLARDAVRVQSLNRNLELSLNGGQKI 120

Query: 122 GKRINGSASTNSLTAPVTSGASHGDG-EYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 181
           G  INGS S +S+TAPV SG S G+G EYFARIGVGQPVQSF+LVPDTGSDVTWLQC+PC
Sbjct: 121 GAGINGSESADSITAPVVSGQSLGNGGEYFARIGVGQPVQSFYLVPDTGSDVTWLQCQPC 180

Query: 182 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 241
           A E AC+KQ+DPIFDPKSSSSY++LSCNS+QCQLLD+A C+S+ C Y+V YGDGSFT GE
Sbjct: 181 AAEKACYKQIDPIFDPKSSSSYTALSCNSQQCQLLDKANCNSDVCQYQVNYGDGSFTTGE 240

Query: 242 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 301
           LATETLSFGNSNSIPNLPIGCGHDNEGLF  AAGLIGLGGGAISLSSQL+ASSFSYCLVD
Sbjct: 241 LATETLSFGNSNSIPNLPIGCGHDNEGLFVGAAGLIGLGGGAISLSSQLKASSFSYCLVD 300

Query: 302 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 361
           LDSDSSSTL+FN D+PSDSLTSPLVKN+RF S+RYVKV+GMSVGG  LPISS+RFEIDES
Sbjct: 301 LDSDSSSTLEFNTDRPSDSLTSPLVKNDRFHSYRYVKVVGMSVGGNPLPISSTRFEIDES 360

Query: 362 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 421
           G GGIIVDSGTTITQLPSDVY+ LR+AFV  T+NLP A G+S FDTCYDLS QSSVEVP+
Sbjct: 361 GLGGIIVDSGTTITQLPSDVYESLREAFVKQTSNLPPAQGISLFDTCYDLSGQSSVEVPV 420

Query: 422 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 481
           IAF+LPG  SL+LPAKN LI VDS GT+CLAF      LSIIG+ QQQGIRVSYDL NS+
Sbjct: 421 IAFVLPGENSLRLPAKNYLIPVDSDGTYCLAFFKTRSSLSIIGSFQQQGIRVSYDLANSL 480

Query: 482 VGFATNKC 488
           VGF+TNKC
Sbjct: 481 VGFSTNKC 487

BLAST of MELO3C017296.jh1 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 7.9e-128
Identity = 244/496 (49.19%), Postives = 333/496 (67.14%), Query Frame = 0

Query: 10  LLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKP--------KPFQ 69
           L  +T+  FL      SR L+  +P  T   DV +S+ Q    LS+ P        KP  
Sbjct: 10  LAVVTLSLFLTTTDASSRSLS--TPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPES 69

Query: 70  THS---YHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGA 129
                 ++S+SPLSL LH R T     +KDY +L  +RL R ++RV  +  K+  ++ G 
Sbjct: 70  LSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGV 129

Query: 130 KQFGKR----INGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTW 189
            +   +     +    T  LT PV SGAS G GEYF+RIGVG P +  +LV DTGSDV W
Sbjct: 130 DRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNW 189

Query: 190 LQCKPCANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDG 249
           +QC+PCA+   C++Q DP+F+P SSS+Y SL+C++ QC LL+ + C SN C+Y+V YGDG
Sbjct: 190 IQCEPCAD---CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDG 249

Query: 250 SFTIGELATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSF 309
           SFT+GELAT+T++FGNS  I N+ +GCGHDNEGLF  AAGL+GLGGG +S+++Q++A+SF
Sbjct: 250 SFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSF 309

Query: 310 SYCLVDLDSDSSSTLDFNADQ-PSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSS 369
           SYCLVD DS  SS+LDFN+ Q      T+PL++N +  +F YV + G SVGG+++ +  +
Sbjct: 310 SYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDA 369

Query: 370 RFEIDESGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPT-APGVSPFDTCYDLSS 429
            F++D SGSGG+I+D GT +T+L +  Y+ LRDAF+ LT NL   +  +S FDTCYD SS
Sbjct: 370 IFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSS 429

Query: 430 QSSVEVPIIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRV 489
            S+V+VP +AF   GGKSL LPAKN LI VD +GTFC AF P +  LSIIGNVQQQG R+
Sbjct: 430 LSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRI 489

BLAST of MELO3C017296.jh1 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 4.7e-96
Identity = 204/487 (41.89%), Postives = 281/487 (57.70%), Query Frame = 0

Query: 10  LLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPFQTH-SYHSN 69
           +L    F FL     L   L++ S  S   F +   +   L   +  P    TH S  S+
Sbjct: 1   MLLPLFFFFLH----LHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDESS 60

Query: 70  SPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQFGKRINGS 129
           S  +L L  R    + +Y+++   + AR+ R   RV ++ R++          GK I  S
Sbjct: 61  SKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRIS---------GKVIPSS 120

Query: 130 AS---TNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPCANENA 189
            S    N   + + SG   G GEYF RIGVG P +  ++V D+GSD+ W+QC+PC     
Sbjct: 121 DSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC---KL 180

Query: 190 CFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGELATET 249
           C+KQ DP+FDP  S SY+ +SC S  C  ++ +GC S  C YEV YGDGS+T G LA ET
Sbjct: 181 CYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALET 240

Query: 250 LSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQ---ASSFSYCLVDLD 309
           L+F  +  + N+ +GCGH N G+F  AAGL+G+GGG++S   QL      +F YCLV   
Sbjct: 241 LTFAKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRG 300

Query: 310 SDSSSTLDFNADQ-PSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDESG 369
           +DS+ +L F  +  P  +   PLV+N R PSF YV + G+ VGG R+P+    F++ E+G
Sbjct: 301 TDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETG 360

Query: 370 SGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPII 429
            GG+++D+GT +T+LP+  Y   RD F   T NLP A GVS FDTCYDLS   SV VP +
Sbjct: 361 DGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTV 420

Query: 430 AFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSIV 489
           +F    G  L LPA+N L+ VD +GT+C AF      LSIIGN+QQ+GI+VS+D  N  V
Sbjct: 421 SFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFV 470

BLAST of MELO3C017296.jh1 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 7.2e-89
Identity = 207/496 (41.73%), Postives = 287/496 (57.86%), Query Frame = 0

Query: 9   ALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQA--------LNALSIKPKPF 68
           ALLF   F FL  PS  S       P   T F  S S+  A         ++ S+    F
Sbjct: 7   ALLFSLCFFFLSLPSFSS------LPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEF 66

Query: 69  QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ 128
           ++ S  S S  S++L+        S K    L  +RL R + RV+S      ++   A+ 
Sbjct: 67  ESGS-DSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKS------IATLAAQI 126

Query: 129 FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 188
            G+ +  +      ++ V SG S G GEYF R+GVG P +  ++V DTGSD+ WLQC PC
Sbjct: 127 PGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC 186

Query: 189 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSS--NSCIYEVEYGDGSFTI 248
                C+ Q DPIFDP+ S +Y+++ C+S  C+ LD AGC++   +C+Y+V YGDGSFT+
Sbjct: 187 ---RRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTV 246

Query: 249 GELATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQ---ASSFS 308
           G+ +TETL+F   N +  + +GCGHDNEGLF  AAGL+GLG G +S   Q        FS
Sbjct: 247 GDFSTETLTF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFS 306

Query: 309 YCLVDLDSDS--SSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLP-ISS 368
           YCLVD  + S  SS +  NA     +  +PL+ N +  +F YV ++G+SVGG R+P +++
Sbjct: 307 YCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTA 366

Query: 369 SRFEIDESGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSS 428
           S F++D+ G+GG+I+DSGT++T+L    Y  +RDAF      L  AP  S FDTC+DLS+
Sbjct: 367 SLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSN 426

Query: 429 QSSVEVPIIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRV 488
            + V+VP +     G   + LPA N LI VD+ G FC AF      LSIIGN+QQQG RV
Sbjct: 427 MNEVKVPTVVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRV 484

BLAST of MELO3C017296.jh1 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 6.1e-72
Identity = 160/397 (40.30%), Postives = 229/397 (57.68%), Query Frame = 0

Query: 99  RHATRVQSLNRKLELSLNGAKQFGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQP 158
           ++ T+ Q L R +E      ++    +NG +        V +    GDGEY   + +G P
Sbjct: 52  KNLTKFQLLERAIERGSRRLQRLEAMLNGPSG-------VETSVYAGDGEYLMNLSIGTP 111

Query: 159 VQSFFLVPDTGSDVTWLQCKPCANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEA 218
            Q F  + DTGSD+ W QC+PC     CF Q  PIF+P+ SSS+S+L C+S+ CQ L   
Sbjct: 112 AQPFSAIMDTGSDLIWTQCQPCTQ---CFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSP 171

Query: 219 GCSSNSCIYEVEYGDGSFTIGELATETLSFGNSNSIPNLPIGCGHDNEGLFDA-AAGLIG 278
            CS+N C Y   YGDGS T G + TETL+FG S SIPN+  GCG +N+G      AGL+G
Sbjct: 172 TCSNNFCQYTYGYGDGSETQGSMGTETLTFG-SVSIPNITFGCGENNQGFGQGNGAGLVG 231

Query: 279 LGGGAISLSSQLQASSFSYCLVDLDSDSSSTLDFNADQPSDSLTSP---LVKNNRFPSFR 338
           +G G +SL SQL  + FSYC+  + S + S L   +   S +  SP   L+++++ P+F 
Sbjct: 232 MGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTTLIQSSQIPTFY 291

Query: 339 YVKVIGMSVGGKRLPISSSRFEID-ESGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTT 398
           Y+ + G+SVG  RLPI  S F ++  +G+GGII+DSGTT+T   ++ Y  +R  F+    
Sbjct: 292 YITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFIS-QI 351

Query: 399 NLPTAPGVSP-FDTCYDL-SSQSSVEVPIIAFILPGGKSLKLPAKNCLIQVDSAGTFCLA 458
           NLP   G S  FD C+   S  S++++P       GG  L+LP++N  I   S G  CLA
Sbjct: 352 NLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELPSENYFIS-PSNGLICLA 411

Query: 459 FLPGTFPLSIIGNVQQQGIRVSYDLDNSIVGFATNKC 489
               +  +SI GN+QQQ + V YD  NS+V FA+ +C
Sbjct: 412 MGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of MELO3C017296.jh1 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 6.4e-69
Identity = 160/404 (39.60%), Postives = 224/404 (55.45%), Query Frame = 0

Query: 92  LVRARLARHATRVQSLNRKLELSLNGAKQFGKRINGSASTNSLTAPVTSGASHGDGEYFA 151
           L++  + R   R++S+N  L+                 S++ +  PV +    GDGEY  
Sbjct: 60  LIKRAIKRGERRMRSINAMLQ-----------------SSSGIETPVYA----GDGEYLM 119

Query: 152 RIGVGQPVQSFFLVPDTGSDVTWLQCKPCANENACFKQLDPIFDPKSSSSYSSLSCNSEQ 211
            + +G P  SF  + DTGSD+ W QC+PC     CF Q  PIF+P+ SSS+S+L C S+ 
Sbjct: 120 NVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQ---CFSQPTPIFNPQDSSSFSTLPCESQY 179

Query: 212 CQLLDEAGCSSNSCIYEVEYGDGSFTIGELATETLSFGNSNSIPNLPIGCGHDNEGLFDA 271
           CQ L    C++N C Y   YGDGS T G +ATET +F  ++S+PN+  GCG DN+G    
Sbjct: 180 CQDLPSETCNNNECQYTYGYGDGSTTQGYMATETFTF-ETSSVPNIAFGCGEDNQGFGQG 239

Query: 272 -AAGLIGLGGGAISLSSQLQASSFSYCLVDLDSDSSSTLDFN---ADQPSDSLTSPLVKN 331
             AGLIG+G G +SL SQL    FSYC+    S S STL      +  P  S ++ L+ +
Sbjct: 240 NGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHS 299

Query: 332 NRFPSFRYVKVIGMSVGGKRLPISSSRFEIDESGSGGIIVDSGTTITQLPSDVYDVLRDA 391
           +  P++ Y+ + G++VGG  L I SS F++ + G+GG+I+DSGTT+T LP D Y+ +  A
Sbjct: 300 SLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQA 359

Query: 392 FVGLTTNLPTA-PGVSPFDTCYDLSSQ-SSVEVPIIAFILPGGKSLKLPAKNCLIQVDSA 451
           F     NLPT     S   TC+   S  S+V+VP I+    GG  L L  +N LI   + 
Sbjct: 360 FTD-QINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGG-VLNLGEQNILIS-PAE 419

Query: 452 GTFCLAF-LPGTFPLSIIGNVQQQGIRVSYDLDNSIVGFATNKC 489
           G  CLA        +SI GN+QQQ  +V YDL N  V F   +C
Sbjct: 420 GVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of MELO3C017296.jh1 vs. ExPASy TrEMBL
Match: A0A5A7UQC3 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003680 PE=3 SV=1)

HSP 1 Score: 954 bits (2467), Expect = 0.0
Identity = 488/488 (100.00%), Postives = 488/488 (100.00%), Query Frame = 0

Query: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60
           MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF
Sbjct: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60

Query: 61  QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ 120
           QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ
Sbjct: 61  QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ 120

Query: 121 FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 180
           FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC
Sbjct: 121 FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 180

Query: 181 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 240
           ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE
Sbjct: 181 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 240

Query: 241 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 300
           LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD
Sbjct: 241 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 300

Query: 301 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 360
           LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES
Sbjct: 301 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 360

Query: 361 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 420
           GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI
Sbjct: 361 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 420

Query: 421 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 480
           IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI
Sbjct: 421 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 480

Query: 481 VGFATNKC 488
           VGFATNKC
Sbjct: 481 VGFATNKC 488

BLAST of MELO3C017296.jh1 vs. ExPASy TrEMBL
Match: A0A1S3BW42 (protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo OX=3656 GN=LOC103494116 PE=3 SV=1)

HSP 1 Score: 954 bits (2467), Expect = 0.0
Identity = 488/488 (100.00%), Postives = 488/488 (100.00%), Query Frame = 0

Query: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60
           MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF
Sbjct: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60

Query: 61  QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ 120
           QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ
Sbjct: 61  QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ 120

Query: 121 FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 180
           FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC
Sbjct: 121 FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 180

Query: 181 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 240
           ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE
Sbjct: 181 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 240

Query: 241 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 300
           LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD
Sbjct: 241 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 300

Query: 301 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 360
           LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES
Sbjct: 301 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 360

Query: 361 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 420
           GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI
Sbjct: 361 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 420

Query: 421 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 480
           IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI
Sbjct: 421 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 480

Query: 481 VGFATNKC 488
           VGFATNKC
Sbjct: 481 VGFATNKC 488

BLAST of MELO3C017296.jh1 vs. ExPASy TrEMBL
Match: A0A5D3DY88 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00360 PE=3 SV=1)

HSP 1 Score: 950 bits (2456), Expect = 0.0
Identity = 486/488 (99.59%), Postives = 487/488 (99.80%), Query Frame = 0

Query: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60
           MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF
Sbjct: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60

Query: 61  QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ 120
           QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ
Sbjct: 61  QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ 120

Query: 121 FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 180
           FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC
Sbjct: 121 FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 180

Query: 181 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 240
           ANENACFKQLDPIFDPK SSSYSSLSC+SEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE
Sbjct: 181 ANENACFKQLDPIFDPKLSSSYSSLSCDSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 240

Query: 241 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 300
           LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD
Sbjct: 241 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 300

Query: 301 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 360
           LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES
Sbjct: 301 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 360

Query: 361 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 420
           GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI
Sbjct: 361 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 420

Query: 421 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 480
           IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI
Sbjct: 421 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 480

Query: 481 VGFATNKC 488
           VGFATNKC
Sbjct: 481 VGFATNKC 488

BLAST of MELO3C017296.jh1 vs. ExPASy TrEMBL
Match: A0A0A0LS14 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G022480 PE=4 SV=1)

HSP 1 Score: 826 bits (2134), Expect = 8.35e-300
Identity = 424/489 (86.71%), Postives = 449/489 (91.82%), Query Frame = 0

Query: 2   MNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPFQ 61
           MNTSLSYA LFLT F  L FPSILSRKLT QSPYST TFDVSASINQALNALSIKPKPFQ
Sbjct: 1   MNTSLSYAFLFLTFFASLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ 60

Query: 62  T-HS-YHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAK 121
           T HS YHS+SPLSLSLHPRLTVHNPSY+DYG+LVRARLAR A R QSLNRKLELSL G K
Sbjct: 61  TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARAQSLNRKLELSLKGGK 120

Query: 122 QFGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKP 181
           QFG+RINGS STNSLTAPVTSGAS G GEYFARIGVGQPVQS+F VPDTGSDV+WLQC+P
Sbjct: 121 QFGRRINGSDSTNSLTAPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQP 180

Query: 182 CANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIG 241
           C  EN C+KQ+ PIFDPKSSSSYS LSC+SEQC LLDEA C +NSCIYEVEYGDGSFT+G
Sbjct: 181 CDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDANSCIYEVEYGDGSFTVG 240

Query: 242 ELATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLV 301
           ELATET SF +SNSIPNLPIGCGHDNEGLF  AAGLIGLGGGAISLSSQL+A+SFSYCLV
Sbjct: 241 ELATETFSFRHSNSIPNLPIGCGHDNEGLFVGAAGLIGLGGGAISLSSQLEATSFSYCLV 300

Query: 302 DLDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDE 361
           DLDS+SSSTLDFNADQPSDSLTSPLVKN+RFP+FRYVKVIGMSVGGK LPISSS FEIDE
Sbjct: 301 DLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDE 360

Query: 362 SGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVP 421
           SGSGGIIVDSGTTIT++PSDVYDVLRDAFVGLT NLP APGVSPFDTCYDLSSQS+VEVP
Sbjct: 361 SGSGGIIVDSGTTITEIPSDVYDVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVP 420

Query: 422 IIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNS 481
            IAFILPG  SL+LPAKNCL QVDSAGTFCLAFLP TFPLSIIGNVQQQGIRVSYDL NS
Sbjct: 421 TIAFILPGENSLQLPAKNCLFQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANS 480

Query: 482 IVGFATNKC 488
           +VGF+T+KC
Sbjct: 481 LVGFSTDKC 489

BLAST of MELO3C017296.jh1 vs. ExPASy TrEMBL
Match: A0A5A7UQC2 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003690 PE=4 SV=1)

HSP 1 Score: 709 bits (1830), Expect = 1.46e-253
Identity = 369/488 (75.61%), Postives = 419/488 (85.86%), Query Frame = 0

Query: 2   MNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPFQ 61
           M TSLS   LFLTIFT LQF SILSRKLT QSPYST+ FDV AS NQALNALSIKPK  Q
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLT-QSPYSTSIFDVLASTNQALNALSIKPKHLQ 60

Query: 62  THSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQF 121
           THS+  NS LSL L+PRL++HNPSYKDY +LVRARLAR A RVQ LNR LE SLNG K F
Sbjct: 61  THSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDF 120

Query: 122 GKRINGSASTNSLTAPVTSGASHGDG-EYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 181
           G+  NGS   +S+TAPV SG S G G EY A++GVGQPV+ F+LVPDTGSDVTWLQC+PC
Sbjct: 121 GEVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPC 180

Query: 182 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 241
           A ENAC+KQ+DPIFDPKSSSSY+ LSCNS+QC LLD   C+S +CIY+V YGDGSFT GE
Sbjct: 181 ATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGE 240

Query: 242 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 301
           LATETLSFGNSNSIPNLPIGCGHDNEGLF   AGLIGLGGGAISLSSQL+ASSFSYCLV+
Sbjct: 241 LATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN 300

Query: 302 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 361
           +DSDSSSTL+FN++ PSDSLTSPLVKN+RF S+RYVKVIG+SVGGK LPISS+RFEIDES
Sbjct: 301 MDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVIGISVGGKTLPISSTRFEIDES 360

Query: 362 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 421
           G GGIIVDSGT I++LPSDVY+ LR+AFV LT++L  APG+S FDTCY+LSSQS+VEVP 
Sbjct: 361 GLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSSQSNVEVPT 420

Query: 422 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 481
           IAF+L GG SL+LPA+N LI+VD+AGT+CLAF+     LSIIG+ QQQGIRVSYDL NS+
Sbjct: 421 IAFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSL 480

Query: 482 VGFATNKC 488
           VGF+TNKC
Sbjct: 481 VGFSTNKC 487

BLAST of MELO3C017296.jh1 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 461.1 bits (1185), Expect = 1.1e-129
Identity = 238/480 (49.58%), Postives = 333/480 (69.38%), Query Frame = 0

Query: 12  FLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPFQTHSYHSNSPL 71
           F  IF      S+ SR L   S  +T+  +V+ SI++     S +    +  ++ ++S  
Sbjct: 8   FFFIFFLTSHSSVFSRILPETSTTTTSILNVADSIHRTKYTSSFRLNQQEEQTHSASSSF 67

Query: 72  SLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNG-AKQFGKRINGSAS 131
           SL LH R++V    + DY +L  ARL R   RV+SL  +L+L++N  +K   K I+   +
Sbjct: 68  SLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLKPISTMYT 127

Query: 132 T--NSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPCANENACFK 191
           T    + AP+ SG + G GEYF R+G+G+P +  ++V DTGSDV WLQC PCA+   C+ 
Sbjct: 128 TEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCAD---CYH 187

Query: 192 QLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGELATETLSF 251
           Q +PIF+P SSSSY  LSC++ QC  L+ + C + +C+YEV YGDGS+T+G+ ATETL+ 
Sbjct: 188 QTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDFATETLTI 247

Query: 252 GNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVDLDSDSSST 311
           G S  + N+ +GCGH NEGLF  AAGL+GLGGG ++L SQL  +SFSYCLVD DSDS+ST
Sbjct: 248 G-STLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDSDSAST 307

Query: 312 LDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDESGSGGIIVD 371
           +DF      D++ +PL++N++  +F Y+ + G+SVGG+ L I  S FE+DESGSGGII+D
Sbjct: 308 VDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGGIIID 367

Query: 372 SGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPIIAFILPGG 431
           SGT +T+L +++Y+ LRD+FV  T +L  A GV+ FDTCY+LS++++VEVP +AF  PGG
Sbjct: 368 SGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPTVAFHFPGG 427

Query: 432 KSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSIVGFATNKC 489
           K L LPAKN +I VDS GTFCLAF P    L+IIGNVQQQG RV++DL NS++GF++NKC
Sbjct: 428 KMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

BLAST of MELO3C017296.jh1 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 458.8 bits (1179), Expect = 5.6e-129
Identity = 244/496 (49.19%), Postives = 333/496 (67.14%), Query Frame = 0

Query: 10  LLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKP--------KPFQ 69
           L  +T+  FL      SR L+  +P  T   DV +S+ Q    LS+ P        KP  
Sbjct: 10  LAVVTLSLFLTTTDASSRSLS--TPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPES 69

Query: 70  THS---YHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGA 129
                 ++S+SPLSL LH R T     +KDY +L  +RL R ++RV  +  K+  ++ G 
Sbjct: 70  LSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGV 129

Query: 130 KQFGKR----INGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTW 189
            +   +     +    T  LT PV SGAS G GEYF+RIGVG P +  +LV DTGSDV W
Sbjct: 130 DRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNW 189

Query: 190 LQCKPCANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDG 249
           +QC+PCA+   C++Q DP+F+P SSS+Y SL+C++ QC LL+ + C SN C+Y+V YGDG
Sbjct: 190 IQCEPCAD---CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDG 249

Query: 250 SFTIGELATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSF 309
           SFT+GELAT+T++FGNS  I N+ +GCGHDNEGLF  AAGL+GLGGG +S+++Q++A+SF
Sbjct: 250 SFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSF 309

Query: 310 SYCLVDLDSDSSSTLDFNADQ-PSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSS 369
           SYCLVD DS  SS+LDFN+ Q      T+PL++N +  +F YV + G SVGG+++ +  +
Sbjct: 310 SYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDA 369

Query: 370 RFEIDESGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPT-APGVSPFDTCYDLSS 429
            F++D SGSGG+I+D GT +T+L +  Y+ LRDAF+ LT NL   +  +S FDTCYD SS
Sbjct: 370 IFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSS 429

Query: 430 QSSVEVPIIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRV 489
            S+V+VP +AF   GGKSL LPAKN LI VD +GTFC AF P +  LSIIGNVQQQG R+
Sbjct: 430 LSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRI 489

BLAST of MELO3C017296.jh1 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 353.2 bits (905), Expect = 3.3e-97
Identity = 204/487 (41.89%), Postives = 281/487 (57.70%), Query Frame = 0

Query: 10  LLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPFQTH-SYHSN 69
           +L    F FL     L   L++ S  S   F +   +   L   +  P    TH S  S+
Sbjct: 1   MLLPLFFFFLH----LHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDESS 60

Query: 70  SPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQFGKRINGS 129
           S  +L L  R    + +Y+++   + AR+ R   RV ++ R++          GK I  S
Sbjct: 61  SKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRIS---------GKVIPSS 120

Query: 130 AS---TNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPCANENA 189
            S    N   + + SG   G GEYF RIGVG P +  ++V D+GSD+ W+QC+PC     
Sbjct: 121 DSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC---KL 180

Query: 190 CFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGELATET 249
           C+KQ DP+FDP  S SY+ +SC S  C  ++ +GC S  C YEV YGDGS+T G LA ET
Sbjct: 181 CYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALET 240

Query: 250 LSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQ---ASSFSYCLVDLD 309
           L+F  +  + N+ +GCGH N G+F  AAGL+G+GGG++S   QL      +F YCLV   
Sbjct: 241 LTFAKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRG 300

Query: 310 SDSSSTLDFNADQ-PSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDESG 369
           +DS+ +L F  +  P  +   PLV+N R PSF YV + G+ VGG R+P+    F++ E+G
Sbjct: 301 TDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETG 360

Query: 370 SGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPII 429
            GG+++D+GT +T+LP+  Y   RD F   T NLP A GVS FDTCYDLS   SV VP +
Sbjct: 361 DGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTV 420

Query: 430 AFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSIV 489
           +F    G  L LPA+N L+ VD +GT+C AF      LSIIGN+QQ+GI+VS+D  N  V
Sbjct: 421 SFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFV 470

BLAST of MELO3C017296.jh1 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 335.9 bits (860), Expect = 5.5e-92
Identity = 212/496 (42.74%), Postives = 287/496 (57.86%), Query Frame = 0

Query: 11  LFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPFQTHSYHSNSP 70
           L  ++F  L F S      +A S Y T   +   S       LS       T    S S 
Sbjct: 9   LAFSVFAVLFFTS------SASSQYQTLVVNTLPS----SATLSWPESESLTDESLSEST 68

Query: 71  LSLSLHPRLTVHNPSYKDY--GTLVRARLARHATRVQSLNRKLELSL--NGAKQFGKRIN 130
            SLS+H        S+ D     L   RL R + RV+S+     +S   N  K+  +   
Sbjct: 69  TSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNATKRTPRTAG 128

Query: 131 GSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPCANENAC 190
           G       +  V SG S G GEYF R+GVG P  + ++V DTGSDV WLQC PC    AC
Sbjct: 129 G------FSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPC---KAC 188

Query: 191 FKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAG-C---SSNSCIYEVEYGDGSFTIGELA 250
           + Q D IFDPK S +++++ C S  C+ LD++  C    S +C+Y+V YGDGSFT G+ +
Sbjct: 189 YNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFS 248

Query: 251 TETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQ---ASSFSYCLV 310
           TETL+F  +  + ++P+GCGHDNEGLF  AAGL+GLG G +S  SQ +      FSYCLV
Sbjct: 249 TETLTFHGAR-VDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLV 308

Query: 311 DLDSDSS-----STLDF-NADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLP-ISS 370
           D  S  S     ST+ F NA  P  S+ +PL+ N +  +F Y++++G+SVGG R+P +S 
Sbjct: 309 DRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSE 368

Query: 371 SRFEIDESGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSS 430
           S+F++D +G+GG+I+DSGT++T+L    Y  LRDAF    T L  AP  S FDTC+DLS 
Sbjct: 369 SQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSG 428

Query: 431 QSSVEVPIIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRV 489
            ++V+VP + F   GG+ + LPA N LI V++ G FC AF      LSIIGN+QQQG RV
Sbjct: 429 MTTVKVPTVVFHFGGGE-VSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRV 483

BLAST of MELO3C017296.jh1 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 329.3 bits (843), Expect = 5.1e-90
Identity = 207/496 (41.73%), Postives = 287/496 (57.86%), Query Frame = 0

Query: 9   ALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQA--------LNALSIKPKPF 68
           ALLF   F FL  PS  S       P   T F  S S+  A         ++ S+    F
Sbjct: 7   ALLFSLCFFFLSLPSFSS------LPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEF 66

Query: 69  QTHSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQ 128
           ++ S  S S  S++L+        S K    L  +RL R + RV+S      ++   A+ 
Sbjct: 67  ESGS-DSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKS------IATLAAQI 126

Query: 129 FGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 188
            G+ +  +      ++ V SG S G GEYF R+GVG P +  ++V DTGSD+ WLQC PC
Sbjct: 127 PGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC 186

Query: 189 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSS--NSCIYEVEYGDGSFTI 248
                C+ Q DPIFDP+ S +Y+++ C+S  C+ LD AGC++   +C+Y+V YGDGSFT+
Sbjct: 187 ---RRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTV 246

Query: 249 GELATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQ---ASSFS 308
           G+ +TETL+F   N +  + +GCGHDNEGLF  AAGL+GLG G +S   Q        FS
Sbjct: 247 GDFSTETLTF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFS 306

Query: 309 YCLVDLDSDS--SSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLP-ISS 368
           YCLVD  + S  SS +  NA     +  +PL+ N +  +F YV ++G+SVGG R+P +++
Sbjct: 307 YCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTA 366

Query: 369 SRFEIDESGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSS 428
           S F++D+ G+GG+I+DSGT++T+L    Y  +RDAF      L  AP  S FDTC+DLS+
Sbjct: 367 SLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSN 426

Query: 429 QSSVEVPIIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRV 488
            + V+VP +     G   + LPA N LI VD+ G FC AF      LSIIGN+QQQG RV
Sbjct: 427 MNEVKVPTVVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRV 484

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008453383.10.0100.00PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis melo] >KAA0058062.... [more]
TYK28408.10.099.59protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis melo var. makuwa][more]
KGN63815.21.81e-30187.12hypothetical protein Csa_013944 [Cucumis sativus][more]
XP_004138237.36.46e-30187.12protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis sativus][more]
XP_038878113.11.06e-26779.10protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9LS407.9e-12849.19Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LHE34.7e-9641.89Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ37.2e-8941.73Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q766C36.1e-7240.30Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C26.4e-6939.60Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5A7UQC30.0100.00Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BW420.0100.00protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo OX=3656 GN=LOC10349411... [more]
A0A5D3DY880.099.59Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A0A0LS148.35e-30086.71Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G02248... [more]
A0A5A7UQC21.46e-25375.61Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11... [more]
Match NameE-valueIdentityDescription
AT1G25510.11.1e-12949.58Eukaryotic aspartyl protease family protein [more]
AT3G18490.15.6e-12949.19Eukaryotic aspartyl protease family protein [more]
AT3G20015.13.3e-9741.89Eukaryotic aspartyl protease family protein [more]
AT3G61820.15.5e-9242.74Eukaryotic aspartyl protease family protein [more]
AT1G01300.15.1e-9041.73Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 123..310
e-value: 8.9E-52
score: 177.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 311..488
e-value: 1.3E-50
score: 173.7
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 143..488
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 149..309
e-value: 1.0E-47
score: 162.7
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 335..484
e-value: 7.5E-36
score: 123.3
NoneNo IPR availablePANTHERPTHR13683:SF274PROTEIN ASPARTIC PROTEASE IN GUARD CELL 1coord: 15..488
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 15..488
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 365..376
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 149..484
score: 46.60437

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C017296.jh1.t1MELO3C017296.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity