MELO3C017219.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C017219.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
DescriptionFlavonoid 3' hydroxylase
Locationchr02: 23078923 .. 23082659 (-)
RNA-Seq ExpressionMELO3C017219.jh1
SyntenyMELO3C017219.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSstart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGTGAATCAATAACACAAAATAAATTTAAATACATATTGCATGGAGGAAATAACCCTCATCGTCGCCGCCATTATAACATTGGCCTCCGCCTTCCTCCTCCACCGCACTTTCTTCCTCCGCCGTAGATTCCGCCTCCCCCCTGGTCCGAAACCATGGCCGATAGTAGGAAACATGCCGCAGATCGGCGGAGCGCCTCTCCACTCTCTCCTTGCCGCCATGGCCGGAAAGTACGGACCACTGATGTACCTCCGCTTAGGCTCAGTGGACATGGTCGTGGCGGCATCCGCGGCTGTGGCAGAGCAAGTCTTGAAGATTCACGACACTAATTTTCTTAGCCGGCCGCCAAACGCCGGTGCAAAATATATTGCTTATAATTATCAGGATATGGTTTTCGCACCGTACGGTCCACGGTGGCGATTGCTCCGGAAGATCAGCGCCGTCCATCTCTTCTCATCAAAAGCTCTAGATGATCATCGCCGTATACGGCAGGTTCGTCTTTATATATATATATATATATTCTTTCTATTTACATGCATGGCATATCCCTGACTTTTTCTTCTCAAAAAACTAAATATCAATAAGTTAGACACTGATTATAGTGTTGATGTTAATTCCAATCAAACTTAAAAGTTGAAAAATACAAAAATCATATATAGATTAAGCTAAAATTAAGGGAGTATTTGACCTAATGAGTGAAGTTTATATACATTTGTGCTTGAAGTGCAAAATTGTAGGATTTAGCGTTTGATAATTATACTAAGGAGGTGGCTGGAATTTAGGATGTGTAGTTGTAATTAAGATTGGAATTATTAATCAAACTAATAACACAAAATTTCATCACACTAATTTTCTAAAAGTAATTTAAATGAGTTAAAGATATTCTATATTTGGTTCTAGAATTGTTTAGTCTATAGTTTTCATACAGATGTTTGTCGACGCCAAACATCTATTGAATATTTTCTTTTATTAGTTAAAAAAATATGAAACAAATATGGTGGAATATATTTAATCTTTTTCCGATGACTTTATTTTTAGGGAAATTAATCCTGTTTGTGTTATAATTAAGATTTTTTTAAAAAATTAGTTTGACCAGAAAATGTAATATATTTAAATATATTTTATATTAATTTAAATAATCAACGTAATTAGATATAAAAATGTGTGCTTTAAATAATGTAAACTGAAAATTAAGTTTTTTTTTTTATAATATTTTTGAAAATATATGTAGTGATGAAGTTAATATTATTTAATACTACATCGTCGTTCAAGTACAGGATGAGATATCGGTGCTAGTACAAGCCCTTGCAAGGTCCGGCGAAGCTCCGGCGAACTTGGGGAGCTTGCTCACCGTGTGCATTGCGAACTCCCTTGGACGGACAATGATCGGGCGGCGTGTTTTCGGTGATGGCAGCGGTAGCGACGATTTAGAGTCAAATCAATTTAAGGCAATGGTGGAGCAAGTGATGGTGCTGGCCGGAAAGTTCAACCCCGGCGACTTTTTCCCATGGCTTGAGAGGCTCGATTTGATGGGTGTGGGCAGTGAGATGAAGAAGGTCCATAAATGGTTCGATGATTTTTTGACTAAGATCGTGGAGGAGCATCGGAATTTGTTGGCCAGAGGTGGCGGTGGTAGTGGCGGCGGCGGCCACCAAGACTTGTTGAGTACTCTTCTGTCGATGAAGGATGAAGGTGTAGATGAGAACGAGAAGCTAAATGATACTGAGATCAAGGCTTTGTTGTTGGTATTATTTCTCTCAATTCTCTCCAAATCTAATTTTCCTCCCAATAAATCAATAGATAAACAATGGATGATTTTTTTGTTAAATTATATACTTTGCCATATAATATATATTTTGAAAAATATAAATTTATTGTTAATTTTTATAAATATAACAAATGATCTGTGGTGTGGTCCGAATAACAAATTAAAATAAAAAAATTTCATGAAGTAAGCTACTTTTTCTAAATATATCAGGTTTGTTCATGTCGCTGTAACTTCGATTTCTTTCTTCTTTCTTTTTTGTTTTTTTTCTTATCTTACAACTTTTCTTTTTCTTTCCATCTTCTTACCTCACTTCTACAATTTTTTATGATATCTGTTTTGAATCAATCATAAATCATAAATCTTGTATGTAATTTTTCTTTTATATATTCTTTTTGAAGAATTATTATCTTTGCTTTCAATCGATATATTTTTTTTTAAGTATTATTTTATTCAAGATCGTGTATAAAATCGTTGGATATTAGCACATGATCGTTAACTATACGACCATGTACCAAATCTAAAGTACAAAATTGCTACCCAAATATAAAGATCTTGGACTTGTAACCAATTAATCGTTTGTTGACCTTCGCATTTTTTATATTTTCTATGGTGGATTTTGGAATTTTTCTGTTTTCAAAATTATTTAAAGTATAAATATTTTGACGATTGGTTATATTTAAAAAAAAAAAAACAAAACCTCAAATTTTTGTAAAATTAACTTTATTTTAATAAATATTTTGAAGATCTCTAAAATAACGATAATAATAATCTATGAAATATATTGGAATGCTAAAACAATTTTCATTACATAACGACAATGTTAATCAATTTCTCACATGGTGATGTTATATTGTCGTTACTATTTCATACAGAACATGTTCACAGCTGGAACCGACACGTCAGCAAGCACAGTTGAATGGGCCTTGACAGAGCTCATAAGACATCCCGAAATGATGGCCCAGGCCCAACAAGAACTGGACTCTGTAGTCGGTCGTGATCGGGCAGTTTCCGATCTGGACTTACCTCAACTAGTCTATCTCCAGGCCGTGGTGAAGGAAACTTTCCGGCTCCACCCACCGACGCCGCTCTCCCTCCCTCGCATGGCGTCCGACAGCTGCGAGGTCAACGGATATCACATCCCCAAAGGCTCCACTCTCTTAGTCGACGTATGGGCCATCGGTCGTGACCCAAAAGAATGGGTCGACCCACTCGAGTTCAGGCCCAATCGGTTCCTCCCCGATGGCGAGAAGCCCCACGTGGACGTAAAGGGAAATGATTTTGAGGCGATTCCGTTTGGGGCTGGGCGGAGAATATGTGTGGGTTTGAGTTTGGGCTTGAGGATGGTCCAGATGTTAACAGCTACGATAGTCCATTCATTTGATTGGGCCTTGCCTAATGGGCTTACACCTGATAAATTGAACATGGATGAGCATTATGGGCTTACTCTTCGTCGGGCCCAACCTTTGGTTGTGCATCCACGGCCCAGGTTATTGCTTGGGGTTTATCAAAAATTGCCTTAAATTTGTTAGTTTTAATTTTTTTTTTCTTTTATTGGGTTTAAGTTTTCTTCTTCTGTTTGATTTTGAAAATTAGTAATAATAGTTTGGGTAAATCGTAAAAACTATCTCAAAAGTATAGTGGTAGCCCTCGAGCTTATCCAAACTTTAAAGCTGAACTCTGAAATTTCAAATTTATATAATCGTATAAATTACACTGATTATATAAGTTTGAATATTTAATTTTCACAAAACTTGAAGTTCCGATTTTAACAATTTTACAAGTTTGGTGATCCATTTATTATTATCGAAAGTTCGAGGGTGTAATTGTAACTACCACCTTCTTTTAATGGTAGTTTTTGTAATTTACACAAATAGTTTATGAGTGCTTGTTCAATATGTCTTTTGTTGGTTTATTGTAATATTAATTATGCCTATTTAAACTTATAAAAATCGTATTATTTACCGGTTTAA

mRNA sequence

GTGTGAATCAATAACACAAAATAAATTTAAATACATATTGCATGGAGGAAATAACCCTCATCGTCGCCGCCATTATAACATTGGCCTCCGCCTTCCTCCTCCACCGCACTTTCTTCCTCCGCCGTAGATTCCGCCTCCCCCCTGGTCCGAAACCATGGCCGATAGTAGGAAACATGCCGCAGATCGGCGGAGCGCCTCTCCACTCTCTCCTTGCCGCCATGGCCGGAAAGTACGGACCACTGATGTACCTCCGCTTAGGCTCAGTGGACATGGTCGTGGCGGCATCCGCGGCTGTGGCAGAGCAAGTCTTGAAGATTCACGACACTAATTTTCTTAGCCGGCCGCCAAACGCCGGTGCAAAATATATTGCTTATAATTATCAGGATATGGTTTTCGCACCGTACGGTCCACGGTGGCGATTGCTCCGGAAGATCAGCGCCGTCCATCTCTTCTCATCAAAAGCTCTAGATGATCATCGCCGTATACGGCAGGATGAGATATCGGTGCTAGTACAAGCCCTTGCAAGGTCCGGCGAAGCTCCGGCGAACTTGGGGAGCTTGCTCACCGTGTGCATTGCGAACTCCCTTGGACGGACAATGATCGGGCGGCGTGTTTTCGGTGATGGCAGCGGTAGCGACGATTTAGAGTCAAATCAATTTAAGGCAATGGTGGAGCAAGTGATGGTGCTGGCCGGAAAGTTCAACCCCGGCGACTTTTTCCCATGGCTTGAGAGGCTCGATTTGATGGGTGTGGGCAGTGAGATGAAGAAGGTCCATAAATGGTTCGATGATTTTTTGACTAAGATCGTGGAGGAGCATCGGAATTTGTTGGCCAGAGGTGGCGGTGGTAGTGGCGGCGGCGGCCACCAAGACTTGTTGAGTACTCTTCTGTCGATGAAGGATGAAGGTGTAGATGAGAACGAGAAGCTAAATGATACTGAGATCAAGGCTTTGTTGTTGAACATGTTCACAGCTGGAACCGACACGTCAGCAAGCACAGTTGAATGGGCCTTGACAGAGCTCATAAGACATCCCGAAATGATGGCCCAGGCCCAACAAGAACTGGACTCTGTAGTCGGTCGTGATCGGGCAGTTTCCGATCTGGACTTACCTCAACTAGTCTATCTCCAGGCCGTGGTGAAGGAAACTTTCCGGCTCCACCCACCGACGCCGCTCTCCCTCCCTCGCATGGCGTCCGACAGCTGCGAGGTCAACGGATATCACATCCCCAAAGGCTCCACTCTCTTAGTCGACGTATGGGCCATCGGTCGTGACCCAAAAGAATGGGTCGACCCACTCGAGTTCAGGCCCAATCGGTTCCTCCCCGATGGCGAGAAGCCCCACGTGGACGTAAAGGGAAATGATTTTGAGGCGATTCCGTTTGGGGCTGGGCGGAGAATATGTGTGGGTTTGAGTTTGGGCTTGAGGATGGTCCAGATGTTAACAGCTACGATAGTCCATTCATTTGATTGGGCCTTGCCTAATGGGCTTACACCTGATAAATTGAACATGGATGAGCATTATGGGCTTACTCTTCGTCGGGCCCAACCTTTGGTTGTGCATCCACGGCCCAGGTTATTGCTTGGGGTTTATCAAAAATTGCCTTAAATTTGTTAGTTTTAATTTTTTTTTTCTTTTATTGGGTTTAAGTTTTCTTCTTCTGTTTGATTTTGAAAATTAGTAATAATAGTTTGGGTAAATCGTAAAAACTATCTCAAAAGTATAGTGGTAGCCCTCGAGCTTATCCAAACTTTAAAGCTGAACTCTGAAATTTCAAATTTATATAATCGTATAAATTACACTGATTATATAAGTTTGAATATTTAATTTTCACAAAACTTGAAGTTCCGATTTTAACAATTTTACAAGTTTGGTGATCCATTTATTATTATCGAAAGTTCGAGGGTGTAATTGTAACTACCACCTTCTTTTAATGGTAGTTTTTGTAATTTACACAAATAGTTTATGAGTGCTTGTTCAATATGTCTTTTGTTGGTTTATTGTAATATTAATTATGCCTATTTAAACTTATAAAAATCGTATTATTTACCGGTTTAA

Coding sequence (CDS)

ATGGAGGAAATAACCCTCATCGTCGCCGCCATTATAACATTGGCCTCCGCCTTCCTCCTCCACCGCACTTTCTTCCTCCGCCGTAGATTCCGCCTCCCCCCTGGTCCGAAACCATGGCCGATAGTAGGAAACATGCCGCAGATCGGCGGAGCGCCTCTCCACTCTCTCCTTGCCGCCATGGCCGGAAAGTACGGACCACTGATGTACCTCCGCTTAGGCTCAGTGGACATGGTCGTGGCGGCATCCGCGGCTGTGGCAGAGCAAGTCTTGAAGATTCACGACACTAATTTTCTTAGCCGGCCGCCAAACGCCGGTGCAAAATATATTGCTTATAATTATCAGGATATGGTTTTCGCACCGTACGGTCCACGGTGGCGATTGCTCCGGAAGATCAGCGCCGTCCATCTCTTCTCATCAAAAGCTCTAGATGATCATCGCCGTATACGGCAGGATGAGATATCGGTGCTAGTACAAGCCCTTGCAAGGTCCGGCGAAGCTCCGGCGAACTTGGGGAGCTTGCTCACCGTGTGCATTGCGAACTCCCTTGGACGGACAATGATCGGGCGGCGTGTTTTCGGTGATGGCAGCGGTAGCGACGATTTAGAGTCAAATCAATTTAAGGCAATGGTGGAGCAAGTGATGGTGCTGGCCGGAAAGTTCAACCCCGGCGACTTTTTCCCATGGCTTGAGAGGCTCGATTTGATGGGTGTGGGCAGTGAGATGAAGAAGGTCCATAAATGGTTCGATGATTTTTTGACTAAGATCGTGGAGGAGCATCGGAATTTGTTGGCCAGAGGTGGCGGTGGTAGTGGCGGCGGCGGCCACCAAGACTTGTTGAGTACTCTTCTGTCGATGAAGGATGAAGGTGTAGATGAGAACGAGAAGCTAAATGATACTGAGATCAAGGCTTTGTTGTTGAACATGTTCACAGCTGGAACCGACACGTCAGCAAGCACAGTTGAATGGGCCTTGACAGAGCTCATAAGACATCCCGAAATGATGGCCCAGGCCCAACAAGAACTGGACTCTGTAGTCGGTCGTGATCGGGCAGTTTCCGATCTGGACTTACCTCAACTAGTCTATCTCCAGGCCGTGGTGAAGGAAACTTTCCGGCTCCACCCACCGACGCCGCTCTCCCTCCCTCGCATGGCGTCCGACAGCTGCGAGGTCAACGGATATCACATCCCCAAAGGCTCCACTCTCTTAGTCGACGTATGGGCCATCGGTCGTGACCCAAAAGAATGGGTCGACCCACTCGAGTTCAGGCCCAATCGGTTCCTCCCCGATGGCGAGAAGCCCCACGTGGACGTAAAGGGAAATGATTTTGAGGCGATTCCGTTTGGGGCTGGGCGGAGAATATGTGTGGGTTTGAGTTTGGGCTTGAGGATGGTCCAGATGTTAACAGCTACGATAGTCCATTCATTTGATTGGGCCTTGCCTAATGGGCTTACACCTGATAAATTGAACATGGATGAGCATTATGGGCTTACTCTTCGTCGGGCCCAACCTTTGGTTGTGCATCCACGGCCCAGGTTATTGCTTGGGGTTTATCAAAAATTGCCTTAA

Protein sequence

MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIANSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSEMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLEFRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP
Homology
BLAST of MELO3C017219.jh1 vs. NCBI nr
Match: XP_008453263.1 (PREDICTED: flavonoid 3'-monooxygenase [Cucumis melo] >KAA0057987.1 flavonoid 3'-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 1043 bits (2697), Expect = 0.0
Identity = 520/521 (99.81%), Postives = 520/521 (99.81%), Query Frame = 0

Query: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60
           MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM
Sbjct: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60

Query: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180
           YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN
Sbjct: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240
           SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240

Query: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300
           MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360
           IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420
           YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420

Query: 421 FRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480
           F PNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP
Sbjct: 421 FGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480

Query: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521
           NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP
Sbjct: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of MELO3C017219.jh1 vs. NCBI nr
Match: XP_004138192.1 (flavonoid 3'-monooxygenase [Cucumis sativus] >KGN63725.2 hypothetical protein Csa_014030 [Cucumis sativus])

HSP 1 Score: 980 bits (2533), Expect = 0.0
Identity = 488/519 (94.03%), Postives = 500/519 (96.34%), Query Frame = 0

Query: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60
           MEEIT IVAA+ITLASAFLLHR FFLRRR RLPPGPKPWPIVGNMPQIG A LHSLLA M
Sbjct: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM 60

Query: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180
           YGPRWRLLRKIS VHLFSSKALDDHRRIR++EISVLVQALARSGEAPANLGSLLTVCIAN
Sbjct: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240
           SLGRTMIGRRVFGDGSGSDDLESNQFK MVEQVMVLAGKFNPGDFFPWLE LDLMGVG E
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE 240

Query: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300
           MKKVHKWFDDFLTKIVEEHRNLLARG GG    GHQDLLSTLLSMKD+G DENEKLNDTE
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGVGG----GHQDLLSTLLSMKDDGEDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360
           IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDS+VGRDRAVSD+DL QLV
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLV 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420
           YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPK+WVDPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLE 420

Query: 421 FRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480
           FRPNRFLP+GEKPHVDVKGNDFE IPFGAGRRICVGLSLGLRMVQMLTATIVHSFDW LP
Sbjct: 421 FRPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLP 480

Query: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQK 519
           NGLTPDKLNMDEHYGLTLRRAQPL++HPRPRLLLGVY++
Sbjct: 481 NGLTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYER 515

BLAST of MELO3C017219.jh1 vs. NCBI nr
Match: XP_038878698.1 (flavonoid 3'-monooxygenase [Benincasa hispida])

HSP 1 Score: 916 bits (2368), Expect = 0.0
Identity = 456/521 (87.52%), Postives = 482/521 (92.51%), Query Frame = 0

Query: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60
           M+EI LIVAA++TLAS  LLHRTFF R RFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM
Sbjct: 1   MDEIALIVAAVVTLASTILLHRTFFRRSRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60

Query: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AG+YG LMYLRLGSV +VVAASAAVAE++LK+HDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGEYGSLMYLRLGSVGVVVAASAAVAEKLLKVHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180
           YGPRWRLLRKIS VHLFSSKALDDHRRIR++EI+VLVQALA SGE PA LGSLLTVC AN
Sbjct: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEIAVLVQALASSGEVPAALGSLLTVCTAN 180

Query: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240
           +LGRTMIGRRVFGDG   DD E+ QFKAMVEQVM+LAGKFNPGDFFP LE LDL+GV SE
Sbjct: 181 ALGRTMIGRRVFGDGGSGDDSEAKQFKAMVEQVMILAGKFNPGDFFPSLEWLDLLGVASE 240

Query: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300
           MKKVHKWFDDFLTKIVEEHRNLLARG GG    GHQDLLSTLLS  +   DENEK+NDTE
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGRGG----GHQDLLSTLLSSAE---DENEKINDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360
           IKALLLNMFTAGTDTSASTVEWALTELIRHP+MMAQAQQELDSVVGRDRAVSDLDLPQLV
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSVVGRDRAVSDLDLPQLV 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420
           YLQAVVKETFRLHPPTPLSLPRMA++SCE+NGYHIPKG+TLLVDVWAIGRDPKEW DPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGTTLLVDVWAIGRDPKEWADPLE 420

Query: 421 FRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480
           FRPNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+S+GLRMVQMLTAT+VH FDWALP
Sbjct: 421 FRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLVHYFDWALP 480

Query: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521
           NGL PD+LNMDE YGLTLRRAQPLVVHPRPRLL GVYQK P
Sbjct: 481 NGLLPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKSP 514

BLAST of MELO3C017219.jh1 vs. NCBI nr
Match: XP_022933611.1 (flavonoid 3'-monooxygenase [Cucurbita moschata])

HSP 1 Score: 863 bits (2229), Expect = 6.21e-313
Identity = 427/521 (81.96%), Postives = 466/521 (89.44%), Query Frame = 0

Query: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFR---LPPGPKPWPIVGNMPQIGGAPLHSLL 60
           M+EI+L  +A ITLAS  LL+R  F +R  R   LPPGPKPWPIVGN+PQIGGAPLHSLL
Sbjct: 1   MDEISLFFSAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLL 60

Query: 61  AAMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120
           A M+G+YGPLMYLR GSVD+VVAASAA+AEQ LKI DTNFLSRPPNAGAKYIAYNYQDMV
Sbjct: 61  ADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVC 180
           FAPYGPRWRLLRKISAVHLFSSKAL+DHRR+R++E+ VLVQALARSGEAPA LGSLLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVC 180

Query: 181 IANSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGV 240
            AN+LGRTMIGRRVFGDGSG  D  S+QFKAMVEQVM+L+GKFNPGDFF WLE LDL+GV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLGV 240

Query: 241 GSEMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLN 300
             EMKKVHKWFDD ++KI+EEHRNLLA GGGG     HQDLLSTLLS +D   DENEKLN
Sbjct: 241 AGEMKKVHKWFDDLMSKILEEHRNLLATGGGGE----HQDLLSTLLSTRDRAKDENEKLN 300

Query: 301 DTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLP 360
           DTEIKALLLNMFTAGTDTSASTVEWAL ELIRHP+MM QAQQELDSVVGRDR VSDLDLP
Sbjct: 301 DTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLP 360

Query: 361 QLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVD 420
           +L YLQAVVKETFRLHPPTPLSLPRMA++SCE++GYHIPKGSTLLVDVWAI RDPKEW  
Sbjct: 361 KLGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTS 420

Query: 421 PLEFRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDW 480
           PLEFRP+RFLP GEK HVDVKGNDF  IPFGAGRRICVG+S+GLRMVQMLTA++VHSFDW
Sbjct: 421 PLEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDW 480

Query: 481 ALPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQ 518
           ALP+GL+P +LNMDE +GLTLRRAQPLV HPRPRL  GVYQ
Sbjct: 481 ALPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

BLAST of MELO3C017219.jh1 vs. NCBI nr
Match: XP_023529494.1 (flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 860 bits (2223), Expect = 5.70e-312
Identity = 427/521 (81.96%), Postives = 468/521 (89.83%), Query Frame = 0

Query: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFR---LPPGPKPWPIVGNMPQIGGAPLHSLL 60
           M+EI+L  +A ITLAS  LL+R  F +R  R   LPPGPKPWPIVGN+PQIGGAPLHSLL
Sbjct: 1   MDEISLFFSAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLL 60

Query: 61  AAMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120
           A M+G+YGPLMYLR GSVD+VVAASAA+AEQ LKI DTNFLSRPPNAGAKYIAYNYQDMV
Sbjct: 61  ADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVC 180
           FAPYGPRWRLLRKISAVHLFSSKAL+DHRR+R++E+ VLVQALARSGEAPA LGSLLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVC 180

Query: 181 IANSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGV 240
            AN+LGRTMIGRRVFGDGSG  D  S+QFKAMVEQVM+L+GKFNPGDFFP L+ LDL+GV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPSLDWLDLLGV 240

Query: 241 GSEMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLN 300
             EMKKVHKWFDD L+KI+EEHRNLLA GGGG GGG HQDLLSTLLS +D   DENEKLN
Sbjct: 241 AREMKKVHKWFDDLLSKILEEHRNLLASGGGG-GGGEHQDLLSTLLSARDRAEDENEKLN 300

Query: 301 DTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLP 360
           DTEIKALLLNMFTAGTDTSASTVEWAL ELIRHP+MM QAQQELDS+VGRDR VSDLDLP
Sbjct: 301 DTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMHQAQQELDSIVGRDRVVSDLDLP 360

Query: 361 QLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVD 420
           +L YLQAVVKETFRLHPPTPLSLPRMA++SCE++GY IPKGSTLLVDVWAI RDPKEW  
Sbjct: 361 KLGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYRIPKGSTLLVDVWAISRDPKEWTS 420

Query: 421 PLEFRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDW 480
           PLEFRP+RFLP GEK HVDVKGNDF  IPFGAGRRICVG+S+GLRMVQ+LTA++VHSFDW
Sbjct: 421 PLEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQILTASLVHSFDW 480

Query: 481 ALPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQ 518
           ALP+GL+P +LNMDE +GLTLRRAQPLV HPRPRL  GVYQ
Sbjct: 481 ALPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 520

BLAST of MELO3C017219.jh1 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 8.6e-189
Identity = 325/519 (62.62%), Postives = 403/519 (77.65%), Query Frame = 0

Query: 1   MEEITLIVAAII-TLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           ME ++LI+  +I +    F+L   F  R    LPPGPKPWPI+GN+  +G  P H   AA
Sbjct: 1   MEILSLILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKP-HQSTAA 60

Query: 61  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120
           MA  YGPLMYL++G VD+VVAASA+VA Q LK HD NF SRPPN+GA+++AYNYQD+VFA
Sbjct: 61  MAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFA 120

Query: 121 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 180
           PYGPRWR+LRKI +VHLFS+KALDD R +RQDE+  L +ALA +G+ P  LG LL VC  
Sbjct: 121 PYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTT 180

Query: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 240
           N+L R M+G+RVF DGSG  D ++ +FK+MV ++MV+AG FN GDF P L  LD+ GV +
Sbjct: 181 NALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAA 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVD-ENEKLND 300
           +MKK+H  FD FLT I+EEH+        G   G  +DLLSTL+S+K++  D +  KL D
Sbjct: 241 KMKKLHARFDAFLTDILEEHK--------GKIFGEMKDLLSTLISLKNDDADNDGGKLTD 300

Query: 301 TEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQ 360
           TEIKALLLN+F AGTDTS+STVEWA+ ELIR+P+++AQAQQE+D VVGRDR V +LDL Q
Sbjct: 301 TEIKALLLNLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQ 360

Query: 361 LVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDP 420
           L YL+A+VKETFRLHP TPLSLPR+AS+SCE+NGY IPKGSTLL++VWAI RDP  W DP
Sbjct: 361 LTYLEAIVKETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADP 420

Query: 421 LEFRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWA 480
           LEFRP RFLP GEKP VDV+GNDFE IPFGAGRRIC G++LG+RMVQ++ AT++H+F+W 
Sbjct: 421 LEFRPERFLPGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWD 480

Query: 481 LPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVY 518
           L +G  P+ LNM+E YGLTL+RA PLVVHPRPRL    Y
Sbjct: 481 LVSGQLPEMLNMEEAYGLTLQRADPLVVHPRPRLEAQAY 510

BLAST of MELO3C017219.jh1 vs. ExPASy Swiss-Prot
Match: Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 4.1e-183
Identity = 318/519 (61.27%), Postives = 396/519 (76.30%), Query Frame = 0

Query: 4   ITLIVAAIITLASAFLLHRTFFLRR----RFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 63
           +T+++A ++     FL+ R F  RR      RLPPGP PWPI+GN+P +G  P H  L+A
Sbjct: 6   LTILLATVL-----FLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSA 65

Query: 64  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 123
           M   YGP+++LRLG VD+VVAAS +VAEQ LKIHD NF SRPPN+GAK++AYNYQD+VFA
Sbjct: 66  MVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFA 125

Query: 124 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 183
           PYG RWRLLRKIS+VHLFS+KAL+D + +RQ+E+  L + L R G  P NLG L+ +C+ 
Sbjct: 126 PYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVV 185

Query: 184 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 243
           N+LGR MIGRR+FG      D ++++F++MV ++M LAG FN GDF P L+ LDL GV  
Sbjct: 186 NALGREMIGRRLFG---ADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAG 245

Query: 244 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVD-ENEKLND 303
           +MK++HK FD FL+ I++EH               H D+LSTL+S+K   +D +   L D
Sbjct: 246 KMKRLHKRFDAFLSSILKEHEM-------NGQDQKHTDMLSTLISLKGTDLDGDGGSLTD 305

Query: 304 TEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQ 363
           TEIKALLLNMFTAGTDTSASTV+WA+ ELIRHP++M +AQ+ELD VVGRDR V++ D+ Q
Sbjct: 306 TEIKALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQ 365

Query: 364 LVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDP 423
           L YLQAV+KE FRLHPPTPLSLP +AS+SCE+NGYHIPKGSTLL ++WAI RDP +W DP
Sbjct: 366 LPYLQAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDP 425

Query: 424 LEFRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWA 483
           L F+P RFLP GEK  VDVKG+DFE IPFGAGRRIC GLSLGLR +Q LTAT+V  FDW 
Sbjct: 426 LAFKPERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWE 485

Query: 484 LPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVY 518
           L  G+TP+KLNM+E YGLTL+RA PLVVHP+PRL   VY
Sbjct: 486 LAGGVTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of MELO3C017219.jh1 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 5.2e-170
Identity = 302/505 (59.80%), Postives = 379/505 (75.05%), Query Frame = 0

Query: 14  LASAFLLHRTFFLRRRFR----LPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGKYGPLMY 73
           L S+F+++  +  RRR      LPPGP  WPI+GN+PQ+G  P H  L A++ +YGPL  
Sbjct: 13  LLSSFIVYLLY--RRRSAQCPPLPPGPNGWPILGNLPQLGAKP-HQTLDALSKQYGPLFR 72

Query: 74  LRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLR 133
           LRLGSV++VVA+S+AVA Q L+ HD NF +RPPN+GA+++AYNYQD+VFAPYGPRWR+LR
Sbjct: 73  LRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRWRMLR 132

Query: 134 KISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAP--ANLGSLLTVCIANSLGRTMI 193
           K+ +VHLFS KALDD R +RQ E++ LV+ L R  +     NLG  L VC  N+L R M+
Sbjct: 133 KLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCATNALARAML 192

Query: 194 GRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSEMKKVHKW 253
           GRRVF D   +   E+++FK MV ++M LAG FN GDF P L  LDL GV  +MK++H+ 
Sbjct: 193 GRRVFAD-EDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKRLHRR 252

Query: 254 FDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTEIKALLLN 313
           +D FL +++EE++     G          DLLS L+ +K+   +   KLN+T+IKALLLN
Sbjct: 253 YDAFLDRVIEENQANAKSG----------DLLSVLIRLKEADAEGEIKLNNTDIKALLLN 312

Query: 314 MFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLVYLQAVVK 373
           +FTAGTDTS+STVEW L ELIRHP+++ + Q ELDSV+GRDR V++ DLP L YLQAVVK
Sbjct: 313 LFTAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPYLQAVVK 372

Query: 374 ETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLEFRPNRFL 433
           ETFRLHP TPLSLPRMAS+ C VNGY IPK +TLLV+VW+IGRD   W DPLEFRP+RFL
Sbjct: 373 ETFRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEFRPSRFL 432

Query: 434 PDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALPNGLTPDK 493
           P GE+ HVDVKGNDFE IPFGAGRRIC GLSLGLRMVQ +TATIVH++DW+LP G    K
Sbjct: 433 PGGEREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPKGQECQK 492

Query: 494 LNMDEHYGLTLRRAQPLVVHPRPRL 513
           L+M+E YGLTL+RA PL+V P PRL
Sbjct: 493 LDMEEAYGLTLQRAVPLMVQPIPRL 503

BLAST of MELO3C017219.jh1 vs. ExPASy Swiss-Prot
Match: Q7G602 (Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B3 PE=2 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 1.3e-160
Identity = 284/494 (57.49%), Postives = 364/494 (73.68%), Query Frame = 0

Query: 27  RRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGKYGPLMYLRLGSVDMVVAASAAVA 86
           R+R  LPPGP+ WP++GN+PQ+G  P H  + A+A +YGPL  LR G  ++VVAASA VA
Sbjct: 36  RKRRPLPPGPRGWPVLGNLPQLGDKP-HHTMCALARQYGPLFRLRFGCAEVVVAASAPVA 95

Query: 87  EQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKISAVHLFSSKALDDHR 146
            Q L+ HD NF +RPPN+GA+++AYNYQD+VFAPYG RWR LRK+ A+HLFS+KALDD R
Sbjct: 96  AQFLRGHDANFSNRPPNSGAEHVAYNYQDLVFAPYGARWRALRKLCALHLFSAKALDDLR 155

Query: 147 RIRQDEISVLVQALARSGEAPANLGSLLTVCIANSLGRTMIGRRVFGDGSGSDDLESNQF 206
            +R+ E++++V+ LAR   A   LG    VC  N+L R  IG RVF    G     + +F
Sbjct: 156 AVREGEVALMVRNLARQQAASVALGQEANVCATNTLARATIGHRVFAVDGGEG---AREF 215

Query: 207 KAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSEMKKVHKWFDDFLTKIVEEHRNLLARG 266
           K MV ++M LAG FN GDF P L  LD  GV ++MK++H+ +D+ +   + E R   A+ 
Sbjct: 216 KEMVVELMQLAGVFNVGDFVPALRWLDPQGVVAKMKRLHRRYDNMMNGFINE-RKAGAQP 275

Query: 267 GGGSGGGGHQDLLSTLLS--MKDEGVD-ENEKLNDTEIKALLLNMFTAGTDTSASTVEWA 326
            G + G    DLLS LL+   +++ +D + EK+ +T+IKALLLN+FTAGTDT++STVEWA
Sbjct: 276 DGVAAGEHGNDLLSVLLARMQEEQKLDGDGEKITETDIKALLLNLFTAGTDTTSSTVEWA 335

Query: 327 LTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLVYLQAVVKETFRLHPPTPLSLPRM 386
           L ELIRHP+++ +AQ ELD+VVGR R VS+ DLP+L YL AV+KETFRLHP TPLSLPR 
Sbjct: 336 LAELIRHPDVLKEAQHELDTVVGRGRLVSESDLPRLPYLTAVIKETFRLHPSTPLSLPRE 395

Query: 387 ASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLEFRPNRFLPDGEKPHVDVKGNDFE 446
           A++ CEV+GY IPKG+TLLV+VWAI RDP +W DPL+++P+RFLP      VDVKG DF 
Sbjct: 396 AAEECEVDGYRIPKGATLLVNVWAIARDPTQWPDPLQYQPSRFLPGRMHADVDVKGADFG 455

Query: 447 AIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALPNGLTPDKLNMDEHYGLTLRRAQP 506
            IPFGAGRRIC GLS GLRMV ++TAT+VH FDW L NG TPDKLNM+E YGLTL+RA P
Sbjct: 456 LIPFGAGRRICAGLSWGLRMVTLMTATLVHGFDWTLANGATPDKLNMEEAYGLTLQRAVP 515

Query: 507 LVVHPRPRLLLGVY 518
           L+V P PRLL   Y
Sbjct: 516 LMVQPVPRLLPSAY 524

BLAST of MELO3C017219.jh1 vs. ExPASy Swiss-Prot
Match: A0A4D6Q414 (Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B138 PE=1 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 1.2e-158
Identity = 278/529 (52.55%), Postives = 383/529 (72.40%), Query Frame = 0

Query: 3   EITLIVAAIITLASAFLLHRTFFLRRR---FRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 62
           +I L ++AI+ L+  +  +  F   +R    +LPPGPK +P++GN+PQ+G  P H  L A
Sbjct: 7   DIILFISAIVFLSIYY--YNLFSNAKRSNGLKLPPGPKGYPVLGNLPQLGAKP-HQALQA 66

Query: 63  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 122
            +  YGPLM LRLGSVD+VVA+S +VA Q LK +D+NF +RPPN+GA+++A+NY D+VFA
Sbjct: 67  FSRVYGPLMRLRLGSVDLVVASSPSVAAQFLK-NDSNFCARPPNSGAEHMAFNYHDLVFA 126

Query: 123 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQAL---ARSGEAPANLGSLLTV 182
           PYGPRWRLLRK+SAVHL   KALDD++ +R++E++VL + L   +R GE P N+G  + V
Sbjct: 127 PYGPRWRLLRKLSAVHLLGPKALDDNQNVREEELAVLARMLYERSRGGE-PVNVGKEMHV 186

Query: 183 CIANSLGRTMIGRRVF-----GDGSGSDDLE---SNQFKAMVEQVMVLAGKFNPGDFFPW 242
           C  N+L R M+GRRVF     G G   ++ E   + +FK MV +VM LAG FN GDF PW
Sbjct: 187 CSTNALSRAMMGRRVFEKLAVGGGGVEEEEEMKKAEEFKDMVVEVMTLAGVFNIGDFVPW 246

Query: 243 LERLDLMGVGSEMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDE 302
           L+  D+ GV  +MK+VH+ ++ FL K + E R+        S   G  DLLS L+  + +
Sbjct: 247 LKPFDIQGVVRKMKRVHRRYNVFLDKFIAECRS--------SAKPGANDLLSVLIGQRGK 306

Query: 303 GVDENEKLNDTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRD 362
                 ++ DT IKAL+LN+ TAGTDTS+ST+EWALTELIRHP+++ +AQQE+DS VGRD
Sbjct: 307 SDGSGGEITDTAIKALVLNLLTAGTDTSSSTIEWALTELIRHPDILKKAQQEIDSAVGRD 366

Query: 363 RAVSDLDLPQLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAI 422
           R V++ D+P+L YLQA+VKE FR+HP TPLSLPRM+ + C++ GYHIPK STL V++WA+
Sbjct: 367 RLVTESDVPKLPYLQAIVKENFRMHPATPLSLPRMSIEECDIGGYHIPKNSTLFVNIWAM 426

Query: 423 GRDPKEWVDPLEFRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLT 482
           GRDP  W DP+EFRP+RFLP G+  H++V+GN FE +PFGAGRRIC G S+G+R+V    
Sbjct: 427 GRDPSIWPDPMEFRPSRFLPGGQGEHLEVRGNHFELMPFGAGRRICAGTSMGIRVVHSTV 486

Query: 483 ATIVHSFDWALPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVY 518
           AT++H+FDW LP GLT +K++M+E +G++L++A PL+ HP PRL    Y
Sbjct: 487 ATLIHAFDWKLPEGLTAEKIDMEEAFGISLQKAIPLMAHPIPRLAPKAY 522

BLAST of MELO3C017219.jh1 vs. ExPASy TrEMBL
Match: A0A5A7UTD5 (Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002910 PE=3 SV=1)

HSP 1 Score: 1043 bits (2697), Expect = 0.0
Identity = 520/521 (99.81%), Postives = 520/521 (99.81%), Query Frame = 0

Query: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60
           MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM
Sbjct: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60

Query: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180
           YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN
Sbjct: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240
           SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240

Query: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300
           MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360
           IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420
           YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420

Query: 421 FRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480
           F PNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP
Sbjct: 421 FGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480

Query: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521
           NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP
Sbjct: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of MELO3C017219.jh1 vs. ExPASy TrEMBL
Match: A0A1S3BVU8 (flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1)

HSP 1 Score: 1043 bits (2697), Expect = 0.0
Identity = 520/521 (99.81%), Postives = 520/521 (99.81%), Query Frame = 0

Query: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60
           MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM
Sbjct: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60

Query: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180
           YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN
Sbjct: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240
           SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240

Query: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300
           MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360
           IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420
           YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420

Query: 421 FRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480
           F PNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP
Sbjct: 421 FGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480

Query: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521
           NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP
Sbjct: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of MELO3C017219.jh1 vs. ExPASy TrEMBL
Match: A0A0A0LP99 (Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1)

HSP 1 Score: 878 bits (2269), Expect = 0.0
Identity = 445/510 (87.25%), Postives = 459/510 (90.00%), Query Frame = 0

Query: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60
           MEEIT IVAA+ITLASAFLLHR FFLRRR RLPPGPKPWPIVGNMPQIG A LHSLLA M
Sbjct: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM 60

Query: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180
           YGPRWRLLRKIS VHLFSSKALDDHRRIR++EISVLVQALARSGEAPANLGSLLTVCIAN
Sbjct: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240
           SLGRTMIGRRVFGDGSGSDDLESNQFK MVEQVMVLAGKFNPGDFFPWLE LDLMGVG E
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE 240

Query: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300
           MKKVHKWFDDFLTKIVEEHRNLLARG GG    GHQDLLSTLLSMKD+G DENEKLNDTE
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGVGG----GHQDLLSTLLSMKDDGEDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360
           IKALLL +F +                          +  L+  +GRDRAVSD+DL QLV
Sbjct: 301 IKALLLVLFLS--------------------------KFYLNCFIGRDRAVSDVDLHQLV 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420
           YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPK+WVDPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLE 420

Query: 421 FRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480
           FRPNRFLP+GEKPHVDVKGNDFE IPFGAGRRICVGLSLGLRMVQMLTATIVHSFDW LP
Sbjct: 421 FRPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLP 480

Query: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRP 510
           NGLTPDKLNMDEHYGLTLRRAQPL++HPRP
Sbjct: 481 NGLTPDKLNMDEHYGLTLRRAQPLIMHPRP 480

BLAST of MELO3C017219.jh1 vs. ExPASy TrEMBL
Match: A0A6J1EZJ7 (flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV=1)

HSP 1 Score: 863 bits (2229), Expect = 3.01e-313
Identity = 427/521 (81.96%), Postives = 466/521 (89.44%), Query Frame = 0

Query: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFR---LPPGPKPWPIVGNMPQIGGAPLHSLL 60
           M+EI+L  +A ITLAS  LL+R  F +R  R   LPPGPKPWPIVGN+PQIGGAPLHSLL
Sbjct: 1   MDEISLFFSAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLL 60

Query: 61  AAMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120
           A M+G+YGPLMYLR GSVD+VVAASAA+AEQ LKI DTNFLSRPPNAGAKYIAYNYQDMV
Sbjct: 61  ADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVC 180
           FAPYGPRWRLLRKISAVHLFSSKAL+DHRR+R++E+ VLVQALARSGEAPA LGSLLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVC 180

Query: 181 IANSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGV 240
            AN+LGRTMIGRRVFGDGSG  D  S+QFKAMVEQVM+L+GKFNPGDFF WLE LDL+GV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLGV 240

Query: 241 GSEMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLN 300
             EMKKVHKWFDD ++KI+EEHRNLLA GGGG     HQDLLSTLLS +D   DENEKLN
Sbjct: 241 AGEMKKVHKWFDDLMSKILEEHRNLLATGGGGE----HQDLLSTLLSTRDRAKDENEKLN 300

Query: 301 DTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLP 360
           DTEIKALLLNMFTAGTDTSASTVEWAL ELIRHP+MM QAQQELDSVVGRDR VSDLDLP
Sbjct: 301 DTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLP 360

Query: 361 QLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVD 420
           +L YLQAVVKETFRLHPPTPLSLPRMA++SCE++GYHIPKGSTLLVDVWAI RDPKEW  
Sbjct: 361 KLGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTS 420

Query: 421 PLEFRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDW 480
           PLEFRP+RFLP GEK HVDVKGNDF  IPFGAGRRICVG+S+GLRMVQMLTA++VHSFDW
Sbjct: 421 PLEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDW 480

Query: 481 ALPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQ 518
           ALP+GL+P +LNMDE +GLTLRRAQPLV HPRPRL  GVYQ
Sbjct: 481 ALPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

BLAST of MELO3C017219.jh1 vs. ExPASy TrEMBL
Match: A0A6J1L0D6 (flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1)

HSP 1 Score: 840 bits (2171), Expect = 1.89e-304
Identity = 419/521 (80.42%), Postives = 460/521 (88.29%), Query Frame = 0

Query: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFR---LPPGPKPWPIVGNMPQIGGAPLHSLL 60
           M+EI+L  +A ITLA+  LL+R  F +R  R   LPPGPKPWP VGN+PQIGGAPLHS L
Sbjct: 1   MDEISLFFSAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQL 60

Query: 61  AAMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120
           AAM+G+YGPLMYLR GS+D+VVAASAA+AEQ LKI DTNFLSRPPNAGAKYIAYNYQDMV
Sbjct: 61  AAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVC 180
           FAPYGPRWRLLRKISAVHLFSSKAL+D+R +R++E+ VLVQALAR GEAPA LGSLLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTVC 180

Query: 181 IANSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGV 240
            AN+LGRTMIGRRVFGDGSG  D  S+QFKAMVEQVM+L+GKFNPG+FFP LE LDL+GV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGV 240

Query: 241 GSEMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLN 300
             EMKKVHKWFDD L+KI+EEHR LLA GG       HQDLLSTLLS +D   DENEKLN
Sbjct: 241 AREMKKVHKWFDDLLSKILEEHRTLLASGGE------HQDLLSTLLSTRDHAEDENEKLN 300

Query: 301 DTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLP 360
           DTEIKALLLNMFTAGTDTSASTVEWAL ELIRHP+MM QAQQELDSVVGRDR VSDLDLP
Sbjct: 301 DTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLP 360

Query: 361 QLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVD 420
           +L YLQAVVKETFRLHPPTPLSLPRMA++SCE+NGYHIPKGSTLLVDVWAI RDPKEW  
Sbjct: 361 KLGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTC 420

Query: 421 PLEFRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDW 480
           PLEFRP+RFLP GEK HVDVKGNDF  IPFGAGRRICVG+S+GLRMVQMLTA++VHSFDW
Sbjct: 421 PLEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDW 480

Query: 481 ALPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQ 518
           ALP+GL+P +LNMDE +GLTLRRAQPLV HPRPRL  GVYQ
Sbjct: 481 ALPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 515

BLAST of MELO3C017219.jh1 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 642.5 bits (1656), Expect = 2.9e-184
Identity = 318/519 (61.27%), Postives = 396/519 (76.30%), Query Frame = 0

Query: 4   ITLIVAAIITLASAFLLHRTFFLRR----RFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 63
           +T+++A ++     FL+ R F  RR      RLPPGP PWPI+GN+P +G  P H  L+A
Sbjct: 6   LTILLATVL-----FLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSA 65

Query: 64  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 123
           M   YGP+++LRLG VD+VVAAS +VAEQ LKIHD NF SRPPN+GAK++AYNYQD+VFA
Sbjct: 66  MVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFA 125

Query: 124 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 183
           PYG RWRLLRKIS+VHLFS+KAL+D + +RQ+E+  L + L R G  P NLG L+ +C+ 
Sbjct: 126 PYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVV 185

Query: 184 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 243
           N+LGR MIGRR+FG      D ++++F++MV ++M LAG FN GDF P L+ LDL GV  
Sbjct: 186 NALGREMIGRRLFG---ADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAG 245

Query: 244 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVD-ENEKLND 303
           +MK++HK FD FL+ I++EH               H D+LSTL+S+K   +D +   L D
Sbjct: 246 KMKRLHKRFDAFLSSILKEHEM-------NGQDQKHTDMLSTLISLKGTDLDGDGGSLTD 305

Query: 304 TEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQ 363
           TEIKALLLNMFTAGTDTSASTV+WA+ ELIRHP++M +AQ+ELD VVGRDR V++ D+ Q
Sbjct: 306 TEIKALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQ 365

Query: 364 LVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDP 423
           L YLQAV+KE FRLHPPTPLSLP +AS+SCE+NGYHIPKGSTLL ++WAI RDP +W DP
Sbjct: 366 LPYLQAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDP 425

Query: 424 LEFRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWA 483
           L F+P RFLP GEK  VDVKG+DFE IPFGAGRRIC GLSLGLR +Q LTAT+V  FDW 
Sbjct: 426 LAFKPERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWE 485

Query: 484 LPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVY 518
           L  G+TP+KLNM+E YGLTL+RA PLVVHP+PRL   VY
Sbjct: 486 LAGGVTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of MELO3C017219.jh1 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 350.1 bits (897), Expect = 3.0e-96
Identity = 191/508 (37.60%), Postives = 293/508 (57.68%), Query Frame = 0

Query: 9   AAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGKYGPLM 68
           A +I  A+  +L   F    +  LPPGP+  PIVGN+P +    LH+    +A  +GP+ 
Sbjct: 21  AVLILTATFSILWYIFKRSPQPPLPPGPRGLPIVGNLPFL-DPDLHTYFTKLAQSHGPIF 80

Query: 69  YLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLL 128
            L LGS   VV  S ++A ++LK  D NF +      A+ + Y   D+V+ PYG  WR+L
Sbjct: 81  KLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRML 140

Query: 129 RKISAVHLFSSKALDDHRRIRQDEISVLVQALARSG--EAPANLGSLLTVCIANSLGRTM 188
           RK+ A  LFS K LD    +R+ EI    + L + G  ++P N+G  L + + N     +
Sbjct: 141 RKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMN-----L 200

Query: 189 IGRRVFGDGSGSDDLES--NQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSEMKKV 248
           +   ++G    ++D+ES   +FK ++ ++  L G  N  DFFP L R DL G+  +M   
Sbjct: 201 MMNMLWGGSVKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVKKMHLY 260

Query: 249 HKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTEIKAL 308
            +  D  L + +E+ + L +R G     G  +D L  L+ ++D+  D +  +    +KA+
Sbjct: 261 ARDLDAILDRAIEQMQRLRSRDG---DDGECKDFLQHLMKLRDQEADSDVPITMNHVKAV 320

Query: 309 LLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLVYLQA 368
           L++M   GT++S +T+E+ + ELI +PE+M +AQQELD VVG+D  V +  +  L Y+ A
Sbjct: 321 LMDMVVGGTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLPYILA 380

Query: 369 VVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLEFRPN 428
           V+KET RL+P  PL +P   S++  V GY IPK + + ++VW+I RDP  W  P EFRP 
Sbjct: 381 VLKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPE 440

Query: 429 RFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALPNGLT 488
           RFL   +K   D  G D+  +PFG+GRRIC G++L  RM+    AT++HSFDW +P+G  
Sbjct: 441 RFL---DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIPDG-- 500

Query: 489 PDKLNMDEHYGLTLRRAQPLVVHPRPRL 513
              L+++E +G+ L+   PLV  P PRL
Sbjct: 501 -HVLDLEEKFGIVLKLKTPLVALPIPRL 513

BLAST of MELO3C017219.jh1 vs. TAIR 10
Match: AT4G22710.1 (cytochrome P450, family 706, subfamily A, polypeptide 2 )

HSP 1 Score: 344.7 bits (883), Expect = 1.3e-94
Identity = 193/512 (37.70%), Postives = 288/512 (56.25%), Query Frame = 0

Query: 4   ITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGK 63
           + L VAA++ +     +      ++   LPPGP P PIVGN+P +    LH+   A+  K
Sbjct: 25  VMLAVAALLAVVCYLWIQGKSKSKKEPPLPPGPWPLPIVGNLPFLNSDVLHTQFQALTLK 84

Query: 64  YGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGP 123
           YGPLM + LGS   +V +S  +A +VLK HD  F +       K   Y  +D++++PYG 
Sbjct: 85  YGPLMKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGGEDILWSPYGT 144

Query: 124 RWRLLRKISAVHLFSSKALDDHRRIRQDEIS---VLVQALARSGEAPANLGSLLTVCIAN 183
            WR LRK+  + +F++ +L+     R++E     V +  +AR G +P NLG  + + I N
Sbjct: 145 HWRRLRKLCVMKMFTTPSLEASYSTRREETRQTIVHMSEMAREG-SPVNLGEQIFLSIFN 204

Query: 184 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 243
            + R M G  V GD   S     N+ K ++  +  + G  N  DFFP   R D  G+  +
Sbjct: 205 VVTRMMWGATVEGDERTS---LGNELKTLISDISDIEGIQNYSDFFPLFSRFDFQGLVKQ 264

Query: 244 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 303
           MK   K  D    +++E H  ++     G      +D L  LL +KD+  DE   L+ T 
Sbjct: 265 MKGHVKKLDLLFDRVMESHVKMV-----GKKSEEEEDFLQYLLRVKDD--DEKAPLSMTH 324

Query: 304 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 363
           +K+LL++M   G DTS +  E+A+ E++  PE++ + + ELD VVG+D  V +  LP+L 
Sbjct: 325 VKSLLMDMVLGGVDTSVNASEFAMAEIVSRPEVLNKIRLELDQVVGKDNIVEESHLPKLP 384

Query: 364 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 423
           YLQAV+KET RLHP  PL +P   S++  V GY +PK S + ++VWAI RDPK W +P E
Sbjct: 385 YLQAVMKETLRLHPTLPLLVPHRNSETSVVAGYTVPKDSKIFINVWAIHRDPKNWDEPNE 444

Query: 424 FRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 483
           F+P RFL +     +D  G DF+ +PFG+GRRIC  +++  R+V    A+++HSFDW  P
Sbjct: 445 FKPERFLENS----LDFNGGDFKYLPFGSGRRICAAINMAERLVLFNIASLLHSFDWKAP 504

Query: 484 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRL 513
            G    K  ++E +GL L+   PLV  P PRL
Sbjct: 505 QG---QKFEVEEKFGLVLKLKSPLVAIPVPRL 518

BLAST of MELO3C017219.jh1 vs. TAIR 10
Match: AT4G22690.1 (cytochrome P450, family 706, subfamily A, polypeptide 1 )

HSP 1 Score: 342.0 bits (876), Expect = 8.2e-94
Identity = 193/512 (37.70%), Postives = 287/512 (56.05%), Query Frame = 0

Query: 4   ITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGK 63
           + L VAA++ +   F +      +    LPPGP P PIVGN+P +    LH+   A+  K
Sbjct: 56  VMLGVAALLAVVCYFWIQGKSKSKNGPPLPPGPWPLPIVGNLPFLNSDVLHTQFQALTLK 115

Query: 64  YGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGP 123
           +GPLM + LGS   +V +S  +A +VLK HD  F +       K   Y  +D++++PYG 
Sbjct: 116 HGPLMKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGGEDILWSPYGT 175

Query: 124 RWRLLRKISAVHLFSSKALDDHRRIRQDEIS---VLVQALARSGEAPANLGSLLTVCIAN 183
            WR LRK+  + +F++  L+     R++E     V +  +AR G +P NLG  + + I N
Sbjct: 176 HWRRLRKLCVMKMFTTPTLEASYSTRREETRQTIVHMSEMAREG-SPVNLGEQIFLSIFN 235

Query: 184 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 243
            + R M G  V GD   S     N+ K ++  +  + G  N  DFFP   R D  G+  +
Sbjct: 236 VVTRMMWGATVEGDERTS---LGNELKTLISDISDIEGIQNYSDFFPLFSRFDFQGLVKQ 295

Query: 244 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 303
           MK   K  D    +++E H  ++     G      +D L  LL +KD+  DE   L+ T 
Sbjct: 296 MKGHVKKLDLLFDRVMESHVKMV-----GKKSEEEEDFLQYLLRVKDD--DEKAPLSMTH 355

Query: 304 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 363
           +K+LL++M   G DTS +  E+A+ E++  PE++ + + ELD VVG+D  V +  LP+L 
Sbjct: 356 VKSLLMDMVLGGVDTSVNASEFAMAEIVSRPEVLNKIRLELDQVVGKDNIVEESHLPKLP 415

Query: 364 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 423
           YLQAV+KET RLHP  PL +P   S++  V GY +PK S + ++VWAI RDPK W +P E
Sbjct: 416 YLQAVMKETLRLHPTLPLLVPHRNSETSVVAGYTVPKDSKIFINVWAIHRDPKNWDEPNE 475

Query: 424 FRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 483
           F+P RFL +     +D  G DF+ +PFG+GRRIC  +++  R+V    A+++HSFDW  P
Sbjct: 476 FKPERFLENS----LDFNGGDFKYLPFGSGRRICAAINMAERLVLFNIASLLHSFDWKAP 535

Query: 484 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRL 513
            G    K  ++E +GL L+   PLV  P PRL
Sbjct: 536 QG---QKFEVEEKFGLVLKLKSPLVAIPVPRL 549

BLAST of MELO3C017219.jh1 vs. TAIR 10
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6 )

HSP 1 Score: 342.0 bits (876), Expect = 8.2e-94
Identity = 190/514 (36.96%), Postives = 293/514 (57.00%), Query Frame = 0

Query: 4   ITLIVAAIITLASAFLLHRTFFLRR-RFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAG 63
           I L   AI+ L + F +    F R  +  LPPGP+  PIVGN+P +    LH+    +A 
Sbjct: 13  INLTPYAILILIAIFSILWYLFKRSPQPHLPPGPRGLPIVGNLPFL-DPDLHTYFTKLAE 72

Query: 64  KYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYG 123
            YGP+  L LGS   VV  + ++A ++LK  D NF +      A+ + Y   D+V+ PYG
Sbjct: 73  SYGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYG 132

Query: 124 PRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSG--EAPANLGSLLTVCIAN 183
             WR+LRK+  + L S + L+    +R+ EI    + L + G  E+P N+G  + + + N
Sbjct: 133 AEWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMMN 192

Query: 184 SLGRTMIGRRVFGDGSGSDDLES--NQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVG 243
                +    ++G    ++++ES   +FK ++ ++  L G+ N  DFFP L R DL G+ 
Sbjct: 193 -----LTMNMLWGGSVKAEEMESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLV 252

Query: 244 SEMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLND 303
            +M    +  D  L + +E+ + L  R G     G  +D L  L+ +KD+  D    +  
Sbjct: 253 KKMHVCARELDAILDRAIEQMQLLRTRDG---DDGECKDFLQHLMKLKDQEADSEVPITV 312

Query: 304 TEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQ 363
             +KA+L+++   GTDTS +T+E+A+ ELIR PE+M +AQQELD VVG+D  + +  + +
Sbjct: 313 NHVKAVLVDLVVGGTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHITR 372

Query: 364 LVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDP 423
           L ++ A++KET RL+P  PL +P   S++  V GY IPK + + ++VW+I RDP  W  P
Sbjct: 373 LPFISAIMKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYP 432

Query: 424 LEFRPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWA 483
            EFRP RFL   +K   D  G D+  +PFG+GRRIC G++L  RM+    AT++HSFDW 
Sbjct: 433 TEFRPERFL---DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWK 492

Query: 484 LPNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRL 513
           +P G     L++ E +G+ L+   PLV  P PRL
Sbjct: 493 IPEG---HILDLKEKFGIVLKLKSPLVALPVPRL 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008453263.10.099.81PREDICTED: flavonoid 3'-monooxygenase [Cucumis melo] >KAA0057987.1 flavonoid 3'-... [more]
XP_004138192.10.094.03flavonoid 3'-monooxygenase [Cucumis sativus] >KGN63725.2 hypothetical protein Cs... [more]
XP_038878698.10.087.52flavonoid 3'-monooxygenase [Benincasa hispida][more]
XP_022933611.16.21e-31381.96flavonoid 3'-monooxygenase [Cucurbita moschata][more]
XP_023529494.15.70e-31281.96flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoi... [more]
Match NameE-valueIdentityDescription
Q9SBQ98.6e-18962.62Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1[more]
Q9SD854.1e-18361.27Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1[more]
A0A4D6Q4155.2e-17059.80Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... [more]
Q7G6021.3e-16057.49Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
A0A4D6Q4141.2e-15852.55Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 G... [more]
Match NameE-valueIdentityDescription
A0A5A7UTD50.099.81Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3BVU80.099.81flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1[more]
A0A0A0LP990.087.25Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1[more]
A0A6J1EZJ73.01e-31381.96flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV... [more]
A0A6J1L0D61.89e-30480.42flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G07990.12.9e-18461.27Cytochrome P450 superfamily protein [more]
AT4G12310.13.0e-9637.60cytochrome P450, family 706, subfamily A, polypeptide 5 [more]
AT4G22710.11.3e-9437.70cytochrome P450, family 706, subfamily A, polypeptide 2 [more]
AT4G22690.18.2e-9437.70cytochrome P450, family 706, subfamily A, polypeptide 1 [more]
AT4G12320.18.2e-9436.96cytochrome P450, family 706, subfamily A, polypeptide 6 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 311..328
score: 38.24
coord: 454..465
score: 27.07
coord: 445..454
score: 60.9
coord: 364..375
score: 45.39
IPR001128Cytochrome P450PFAMPF00067p450coord: 33..489
e-value: 3.6E-105
score: 352.4
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 404..428
score: 38.33
coord: 444..454
score: 56.69
coord: 320..346
score: 35.86
coord: 454..477
score: 24.02
coord: 300..317
score: 32.51
coord: 363..381
score: 40.21
coord: 85..106
score: 22.75
coord: 61..80
score: 35.48
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 26..512
e-value: 1.1E-128
score: 431.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 33..511
NoneNo IPR availablePANTHERPTHR24298:SF45FLAVONOID 3'-MONOOXYGENASEcoord: 10..509
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 10..509
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 447..456

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C017219.jh1.t1MELO3C017219.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen