MELO3C016219.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C016219.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Descriptionabscisic acid 8'-hydroxylase 1
Locationchr07: 21492454 .. 21495083 (-)
RNA-Seq ExpressionMELO3C016219.jh1
SyntenyMELO3C016219.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSstart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTTTTCCTCCCTTCAAAACAACTCCTACTACTTCTAACTTCTAACCATGTACGTCTTCCTCTCCCTCGTTGCCTTCTTCTTCCTTCTTTTCTTTCGTTATCTTTTCAAATTCTCTGCTCCGGCTACCCGGAAACTTCCCCTTCCCCCGGGTTCCATGGGTTGGCCCTATTTGGGTGAGACCCTCCAACTCTACTCTCAAGATCCCAATGTCTTCTTTGCTTCAAAAAAGAAGAGGTCACTTTCTTTTTTTTTACTCTTATTTTCCTTTTGCTTTGGGGCTTTTTTTTTTTTTTTTCTTTGAAGGGGGGGTTTTGATGATGAGTTTTGTTGTTTTGATTTTTGCAGATATGGTCCAATTTTTAAGTCGCATATATTGGGGTATCCTTGCGTGATGCTTTCTAGTCCTGAAGCTGTTAAGTTTGTATTGGTGACTAAAGCTCATCTCTTTAAGCCTACATTTCCAGCTAGTAAAGAGAGGATGTTGGGAAAAAATGCTATTTTTTTTCATCAAGGTGATTACCATGCCAAATTGAGACGTCTTGTTCTTCGAACTTTCATGCCTGAGGCGATTCGAAACATCGTCCCGAGTATCGAATCCATTGCTAAAAACACCGTTCAATCATGGGATGGACAATTGATTAACACCTTTCAAGAAATGAAGATGGTACGTTTAGAAACAGAGCCTCTCTGTTTATTTTAGGTTAAAAACACTCTGTTTCTCTTATCCCCATTTTGAGTAATTGGTGTCTTTAGCTGATTATTTCTTTCCATTCTTCTTTAGTTCGCATTCGAGGTTTCGTTGCTTTCGATATTCGGAAAAGACGAAGCTCTTTACTTCGAAGACCTCAAGCGATGCTACTACATTCTTGAAAATGGCTACAATTCGATGCCGATTAACCTCCCCGGAACACTCTTCCACAAGGCCATGAAAGCAAGGAAAGAGCTAGCACAAATACTAAACAAAATCCTCTCAACAAGAAGGGAAATGAAGCGCGACTACGACGACCTCCTTGGATCTTTCATGGGCGAAAAAGAAGGCCTCACCGACACACAAATCGCCGATAACGTCATCGGTCTCATCTTCGCTGCTCGCGACACCACCGCGAGTGTCCTAACATGGATCTTAAAATACCTAGGAGAAAACCCAAGTATTCTTCAAGCTGTAACAGTAAGAATGATCTCTCAATTTCTTAACCAATTTCCACTTCATTCTTTGAAAAAGTTCAGAAATTTGATTCTCCTCTGTTTTCTGGGTTTGTAGGCTGAACAAGAATCAATAATGAAATCAAAAGAAAATGGAGAAGATAATCTCACATGGGCTGACACCAAAAATATGCCAATAACTTCAAGAGTAATTCAAGAAACTCTAAGAGTGGCTTCAGTTTTATCATTTACTTTTAGAGAAGCAGTGGAAGATGTTGAATTTGATGGTAAGTAAAAAGAAAACCCAAAAAAAGTTAGTTATTTAAATTATGAAATTAAAATCCTTTTCTTTACTTAAATCTTTTTTCTTCTAAATTTGTTATTTTGCAGGATACTTAATTCCAAAAGGATGGAAAGTTTTACCTTTGTTCAGAAACATTCATCACAGCCCAGAAATTTTCCCTCAACCTGAAAAATTTGATCCTTCAAGATTTGAGGTAAAAATAATAATAATAATAAAAAAAAAAAGGACAATATTCATTATTGTTTTTTACTTACTTCATTTAGAAAACACAAACAAATGATTCTGATGGAAATTGTTGGACTTTTTTATTTATTATTATTTTATTATTTATTATAAATTTCCGTTCTTGCATAACAAATTTGGTAAGGCCAAAAATTCAATGATTGTTTTGATATGATTGGAACAGGTTTCACAGAAGCCCAATACATACATGCCATTTGGCAATGGGACCCACTCATGTCCAGGAAATGAATTAGCCAAGCTGGAAATGTTGGTTCTACTCCATCATTTAACCACAAAGTATAGGTAGGTTAATTATTATTATTTTACCATAAGAACAAAAACAAAATTGATTTTAATTATAGTGATTAATTTGTGTTTTTGAATTTGAAATGAATGTAGGTGGAATGTAGTTGGTGAACAAGAAGGGATTCAATATGGTCCTTTTGCACTTCCTCTCAATGGCCTTCCTATTAGAATCTCTCTCAAGAAGTAAATACAAAAAAGACAGAAATAACCCCCCAAACAAAAACAAGAATTTTCCAAGCATTAAAAGAGAAAAAGAAAAATCTGTTCTTTGTTAAGAGGAGAAGAAGAAGAAAAGCCACCTTGAATTTTGGGCAAAAGGGGACAGTTTCTGACTCACAAGAGAGTCATTATTAGTTATAGATTTGGATATACTTTAAAACCCCAATTCAGCCACAAACAATATCTCCAAAAACAACAAAAAATCAACGCTTCTAATCTTTTTGAAGTTTATTCTTTTCTTTCTTTTTTTTTTTTTTTTTGTTAAAGCTTACAAATTTTCATGAGATGAAGAGGAAAAGTCCTCAAGGAATGATTGATGTATTTACTTTCACATACCAATTTTTTGTTTTGTTTTTTTTTTTTTTTTGTAACCATAAAAATGCCGCAAGGCTAATAATATCATATATAATAAAAGTCTATTTTCCGACTTGTAA

mRNA sequence

TATTTTTCCTCCCTTCAAAACAACTCCTACTACTTCTAACTTCTAACCATGTACGTCTTCCTCTCCCTCGTTGCCTTCTTCTTCCTTCTTTTCTTTCGTTATCTTTTCAAATTCTCTGCTCCGGCTACCCGGAAACTTCCCCTTCCCCCGGGTTCCATGGGTTGGCCCTATTTGGGTGAGACCCTCCAACTCTACTCTCAAGATCCCAATGTCTTCTTTGCTTCAAAAAAGAAGAGATATGGTCCAATTTTTAAGTCGCATATATTGGGGTATCCTTGCGTGATGCTTTCTAGTCCTGAAGCTGTTAAGTTTGTATTGGTGACTAAAGCTCATCTCTTTAAGCCTACATTTCCAGCTAGTAAAGAGAGGATGTTGGGAAAAAATGCTATTTTTTTTCATCAAGGTGATTACCATGCCAAATTGAGACGTCTTGTTCTTCGAACTTTCATGCCTGAGGCGATTCGAAACATCGTCCCGAGTATCGAATCCATTGCTAAAAACACCGTTCAATCATGGGATGGACAATTGATTAACACCTTTCAAGAAATGAAGATGTTCGCATTCGAGGTTTCGTTGCTTTCGATATTCGGAAAAGACGAAGCTCTTTACTTCGAAGACCTCAAGCGATGCTACTACATTCTTGAAAATGGCTACAATTCGATGCCGATTAACCTCCCCGGAACACTCTTCCACAAGGCCATGAAAGCAAGGAAAGAGCTAGCACAAATACTAAACAAAATCCTCTCAACAAGAAGGGAAATGAAGCGCGACTACGACGACCTCCTTGGATCTTTCATGGGCGAAAAAGAAGGCCTCACCGACACACAAATCGCCGATAACGTCATCGGTCTCATCTTCGCTGCTCGCGACACCACCGCGAGTGTCCTAACATGGATCTTAAAATACCTAGGAGAAAACCCAAGTATTCTTCAAGCTGTAACAGCTGAACAAGAATCAATAATGAAATCAAAAGAAAATGGAGAAGATAATCTCACATGGGCTGACACCAAAAATATGCCAATAACTTCAAGAGTAATTCAAGAAACTCTAAGAGTGGCTTCAGTTTTATCATTTACTTTTAGAGAAGCAGTGGAAGATGTTGAATTTGATGGATACTTAATTCCAAAAGGATGGAAAGTTTTACCTTTGTTCAGAAACATTCATCACAGCCCAGAAATTTTCCCTCAACCTGAAAAATTTGATCCTTCAAGATTTGAGGTTTCACAGAAGCCCAATACATACATGCCATTTGGCAATGGGACCCACTCATGTCCAGGAAATGAATTAGCCAAGCTGGAAATGTTGGTTCTACTCCATCATTTAACCACAAAGTATAGGTGGAATGTAGTTGGTGAACAAGAAGGGATTCAATATGGTCCTTTTGCACTTCCTCTCAATGGCCTTCCTATTAGAATCTCTCTCAAGAAGTAAATACAAAAAAGACAGAAATAACCCCCCAAACAAAAACAAGAATTTTCCAAGCATTAAAAGAGAAAAAGAAAAATCTGTTCTTTGTTAAGAGGAGAAGAAGAAGAAAAGCCACCTTGAATTTTGGGCAAAAGGGGACAGTTTCTGACTCACAAGAGAGTCATTATTAGTTATAGATTTGGATATACTTTAAAACCCCAATTCAGCCACAAACAATATCTCCAAAAACAACAAAAAATCAACGCTTCTAATCTTTTTGAAGTTTATTCTTTTCTTTCTTTTTTTTTTTTTTTTTGTTAAAGCTTACAAATTTTCATGAGATGAAGAGGAAAAGTCCTCAAGGAATGATTGATGTATTTACTTTCACATACCAATTTTTTGTTTTGTTTTTTTTTTTTTTTTGTAACCATAAAAATGCCGCAAGGCTAATAATATCATATATAATAAAAGTCTATTTTCCGACTTGTAA

Coding sequence (CDS)

ATGTACGTCTTCCTCTCCCTCGTTGCCTTCTTCTTCCTTCTTTTCTTTCGTTATCTTTTCAAATTCTCTGCTCCGGCTACCCGGAAACTTCCCCTTCCCCCGGGTTCCATGGGTTGGCCCTATTTGGGTGAGACCCTCCAACTCTACTCTCAAGATCCCAATGTCTTCTTTGCTTCAAAAAAGAAGAGATATGGTCCAATTTTTAAGTCGCATATATTGGGGTATCCTTGCGTGATGCTTTCTAGTCCTGAAGCTGTTAAGTTTGTATTGGTGACTAAAGCTCATCTCTTTAAGCCTACATTTCCAGCTAGTAAAGAGAGGATGTTGGGAAAAAATGCTATTTTTTTTCATCAAGGTGATTACCATGCCAAATTGAGACGTCTTGTTCTTCGAACTTTCATGCCTGAGGCGATTCGAAACATCGTCCCGAGTATCGAATCCATTGCTAAAAACACCGTTCAATCATGGGATGGACAATTGATTAACACCTTTCAAGAAATGAAGATGTTCGCATTCGAGGTTTCGTTGCTTTCGATATTCGGAAAAGACGAAGCTCTTTACTTCGAAGACCTCAAGCGATGCTACTACATTCTTGAAAATGGCTACAATTCGATGCCGATTAACCTCCCCGGAACACTCTTCCACAAGGCCATGAAAGCAAGGAAAGAGCTAGCACAAATACTAAACAAAATCCTCTCAACAAGAAGGGAAATGAAGCGCGACTACGACGACCTCCTTGGATCTTTCATGGGCGAAAAAGAAGGCCTCACCGACACACAAATCGCCGATAACGTCATCGGTCTCATCTTCGCTGCTCGCGACACCACCGCGAGTGTCCTAACATGGATCTTAAAATACCTAGGAGAAAACCCAAGTATTCTTCAAGCTGTAACAGCTGAACAAGAATCAATAATGAAATCAAAAGAAAATGGAGAAGATAATCTCACATGGGCTGACACCAAAAATATGCCAATAACTTCAAGAGTAATTCAAGAAACTCTAAGAGTGGCTTCAGTTTTATCATTTACTTTTAGAGAAGCAGTGGAAGATGTTGAATTTGATGGATACTTAATTCCAAAAGGATGGAAAGTTTTACCTTTGTTCAGAAACATTCATCACAGCCCAGAAATTTTCCCTCAACCTGAAAAATTTGATCCTTCAAGATTTGAGGTTTCACAGAAGCCCAATACATACATGCCATTTGGCAATGGGACCCACTCATGTCCAGGAAATGAATTAGCCAAGCTGGAAATGTTGGTTCTACTCCATCATTTAACCACAAAGTATAGGTGGAATGTAGTTGGTGAACAAGAAGGGATTCAATATGGTCCTTTTGCACTTCCTCTCAATGGCCTTCCTATTAGAATCTCTCTCAAGAAGTAA

Protein sequence

MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASKKKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFGKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKRDYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAEQESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKGWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK
Homology
BLAST of MELO3C016219.jh1 vs. NCBI nr
Match: XP_008451873.1 (PREDICTED: abscisic acid 8'-hydroxylase 1 [Cucumis melo])

HSP 1 Score: 925 bits (2391), Expect = 0.0
Identity = 460/460 (100.00%), Postives = 460/460 (100.00%), Query Frame = 0

Query: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60
           MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK
Sbjct: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240
           GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR
Sbjct: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240

Query: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300
           DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE
Sbjct: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300

Query: 301 QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460
           LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK
Sbjct: 421 LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460

BLAST of MELO3C016219.jh1 vs. NCBI nr
Match: XP_004150544.2 (abscisic acid 8'-hydroxylase CYP707A2 [Cucumis sativus] >KGN53469.1 hypothetical protein Csa_014563 [Cucumis sativus])

HSP 1 Score: 905 bits (2338), Expect = 0.0
Identity = 447/460 (97.17%), Postives = 456/460 (99.13%), Query Frame = 0

Query: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60
           MYVF+SL+AFFFLLFF YLFKFSAP+ RKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK
Sbjct: 1   MYVFVSLLAFFFLLFFHYLFKFSAPSRRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240
           GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELA+ILNKILSTRRE KR
Sbjct: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAEILNKILSTRRETKR 240

Query: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300
           +YDDLLGSFMGEKEGLTD QIADNVIGLIFAARDTTASVLTWI+KYLGENPSILQAVTAE
Sbjct: 241 EYDDLLGSFMGEKEGLTDKQIADNVIGLIFAARDTTASVLTWIVKYLGENPSILQAVTAE 300

Query: 301 QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QESI+KSKENG+DNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QESIVKSKENGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460
           LLHHLTTK+RWNVVGEQEGIQYGPFALPLNGLPIRISLKK
Sbjct: 421 LLHHLTTKFRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460

BLAST of MELO3C016219.jh1 vs. NCBI nr
Match: XP_038877343.1 (abscisic acid 8'-hydroxylase CYP707A2-like [Benincasa hispida])

HSP 1 Score: 874 bits (2259), Expect = 0.0
Identity = 430/456 (94.30%), Postives = 441/456 (96.71%), Query Frame = 0

Query: 5   LSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASKKKRY 64
           L L+  F LLFF YLFKFSAPA RKLPLPPGSMGWPYLGETLQLYSQDPNVFFASKKKRY
Sbjct: 9   LLLLLLFVLLFFHYLFKFSAPARRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASKKKRY 68

Query: 65  GPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYHAK 124
           GPIFKSHILGYPCVM+SSPEA KFVLVTKAHLFKPTFP SKERMLGKNAIFFHQGDYH+K
Sbjct: 69  GPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPVSKERMLGKNAIFFHQGDYHSK 128

Query: 125 LRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFGKDE 184
           LRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQ INTFQEMKMFAFEVSLLSIFGKDE
Sbjct: 129 LRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQFINTFQEMKMFAFEVSLLSIFGKDE 188

Query: 185 ALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKRDYDD 244
           ALYFEDLKRCYYILE GYNSMPINLPGTLFHKAMKARKELA ILNKILSTRREMKRD++D
Sbjct: 189 ALYFEDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAHILNKILSTRREMKRDHND 248

Query: 245 LLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAEQESI 304
           LLGSFMGEKEGLTD QIADNVIGLIFAARDTTASVLTWILKYLGENPS+LQAVTAEQE+I
Sbjct: 249 LLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTAEQEAI 308

Query: 305 MKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKGWKV 364
           MK K++G+DNLTWADTKNMPITSRVIQETLR+ASVLSFTFREAVEDVEFDGYLIPKGWKV
Sbjct: 309 MKEKQSGDDNLTWADTKNMPITSRVIQETLRMASVLSFTFREAVEDVEFDGYLIPKGWKV 368

Query: 365 LPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHH 424
           LPLFRNIHHSPEIFPQP KFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHH
Sbjct: 369 LPLFRNIHHSPEIFPQPNKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHH 428

Query: 425 LTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460
           LTTKYRWNVVG QEGIQYGPFALPLNGLPIRISLKK
Sbjct: 429 LTTKYRWNVVGAQEGIQYGPFALPLNGLPIRISLKK 464

BLAST of MELO3C016219.jh1 vs. NCBI nr
Match: XP_022931288.1 (abscisic acid 8'-hydroxylase 1-like [Cucurbita moschata] >XP_023553532.1 abscisic acid 8'-hydroxylase 1-like [Cucurbita pepo subsp. pepo] >KAG7015108.1 Abscisic acid 8'-hydroxylase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 860 bits (2223), Expect = 5.18e-314
Identity = 423/460 (91.96%), Postives = 441/460 (95.87%), Query Frame = 0

Query: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60
           M+ F +++A FFLL   +LFKFSAPA RKLPLPPGSMGWPY+GETLQLYSQDPNVFFASK
Sbjct: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVM+SSPEA KFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240
           GK+EALYFEDLKRCYY LE GYNSMPINLPGTLFHKAMKARKELA IL KILSTRREMKR
Sbjct: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240

Query: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300
           D++DLLGSFMGEKEGLTD QIADNVIGLIFAARDTTASVLTWILKYLGENPS+LQAVT E
Sbjct: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300

Query: 301 QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QE+IMK K++G+DNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPE FPQP+KFDPSRFEV QKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460
           LLHHLTTKYRW+VVG QEGIQYGPFALP NGLPI+ISLKK
Sbjct: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 460

BLAST of MELO3C016219.jh1 vs. NCBI nr
Match: XP_022985007.1 (abscisic acid 8'-hydroxylase 1-like [Cucurbita maxima])

HSP 1 Score: 860 bits (2222), Expect = 7.35e-314
Identity = 422/460 (91.74%), Postives = 442/460 (96.09%), Query Frame = 0

Query: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60
           M+ F +++A FFLL   +LFKFSAPA RKLPLPPGSMGWPY+GETLQLYSQDPNVFFASK
Sbjct: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVM+SSPEA KFVLVTKAHLFKPTFPASKERMLGKNAIFFH+GD
Sbjct: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHRGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240
           GK+EALYFEDLKRCYY LE GYNSMPINLPGTLFHKAMKARKELA IL KILSTRREMKR
Sbjct: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240

Query: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300
           D++DLLGSFMGEKEGLTD QIADNVIGLIFAARDTTASVLTWILKYLGENPS+LQAVTAE
Sbjct: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTAE 300

Query: 301 QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QE+IMK K++G+DNLTW+DTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QEAIMKEKQSGDDNLTWSDTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPE FPQP+KFDPSRFEV QKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460
           LLHHLTTKYRW+VVG QEGIQYGPFALP NGLPI+ISLKK
Sbjct: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 460

BLAST of MELO3C016219.jh1 vs. ExPASy Swiss-Prot
Match: K4CI52 (Abscisic acid 8'-hydroxylase CYP707A2 OS=Solanum lycopersicum OX=4081 GN=CYP707A2 PE=2 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 9.5e-208
Identity = 353/461 (76.57%), Postives = 411/461 (89.15%), Query Frame = 0

Query: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60
           ++ F+SL     LL    +FKF A A++KLPLPPG++G PY+GET QLYSQ+PNVFFASK
Sbjct: 9   LFTFISLT----LLLIHSIFKFLAFASKKLPLPPGTLGLPYIGETFQLYSQNPNVFFASK 68

Query: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
            K+YG IFK++ILG PCVM+SSPEA K VLVTKA+LFKPTFPASKERMLGK AIFFHQGD
Sbjct: 69  VKKYGSIFKTYILGCPCVMISSPEAAKQVLVTKANLFKPTFPASKERMLGKQAIFFHQGD 128

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLR+LVL+ F P++IRNI+P IESIA  +++S+ G+LINT+QEMK + F V+L+SIF
Sbjct: 129 YHAKLRKLVLQAFKPDSIRNIIPDIESIAITSLESFQGRLINTYQEMKTYTFNVALISIF 188

Query: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240
           GKDE LY E+LK+CYYILE GYNSMPINLPGTLF+KAMKARKELA+I+ KI+STRREMK 
Sbjct: 189 GKDEFLYREELKKCYYILEKGYNSMPINLPGTLFNKAMKARKELAKIVAKIISTRREMKI 248

Query: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300
           D+ DLLGSFMG+KEGLTD QIADNVIG+IFAARDTTASVLTWILKYLGENPS+LQAVT E
Sbjct: 249 DHGDLLGSFMGDKEGLTDEQIADNVIGVIFAARDTTASVLTWILKYLGENPSVLQAVTEE 308

Query: 301 QESIMKSKE-NGEDN-LTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLI 360
           QE+IM+ KE NGE+  L W DT+ MP+T+RVIQETLRVAS+LSFTFREAVEDVEF+GYLI
Sbjct: 309 QENIMRKKEVNGEEKVLNWQDTRQMPMTTRVIQETLRVASILSFTFREAVEDVEFEGYLI 368

Query: 361 PKGWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEM 420
           PKGWKVLPLFRNIHHSP+ FP+PEKFDPSRFEVS KPNT+MPFGNG HSCPGN+LAKLE+
Sbjct: 369 PKGWKVLPLFRNIHHSPDNFPEPEKFDPSRFEVSPKPNTFMPFGNGVHSCPGNDLAKLEI 428

Query: 421 LVLLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLK 460
           L+L+HHLTTKYRW++VG Q GIQYGPFALP NGLPI++SLK
Sbjct: 429 LILVHHLTTKYRWSMVGPQNGIQYGPFALPQNGLPIKLSLK 465

BLAST of MELO3C016219.jh1 vs. ExPASy Swiss-Prot
Match: Q949P1 (Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 1.3e-204
Identity = 343/458 (74.89%), Postives = 399/458 (87.12%), Query Frame = 0

Query: 3   VFLSLVA-FFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASKK 62
           +FL+L A   FL F R L       + KLPLPPG+MGWPY+GET QLYSQDPNVFF SK+
Sbjct: 6   LFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQ 65

Query: 63  KRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDY 122
           KRYG +FK+H+LG PCVM+SSPEA KFVLVTK+HLFKPTFPASKERMLGK AIFFHQGDY
Sbjct: 66  KRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 125

Query: 123 HAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFG 182
           HAKLR+LVLR FMPE+IRN+VP IESIA+++++SW+G +INT+QEMK + F V+LLSIFG
Sbjct: 126 HAKLRKLVLRAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFG 185

Query: 183 KDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKRD 242
           KDE LY EDLKRCYYILE GYNSMP+NLPGTLFHK+MKARKEL+QIL +ILS RR+    
Sbjct: 186 KDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSS 245

Query: 243 YDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAEQ 302
           ++DLLGSFMG+KE LTD QIADN+IG+IFAARDTTASV++WILKYL ENP++L+AVT EQ
Sbjct: 246 HNDLLGSFMGDKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQ 305

Query: 303 ESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKG 362
            +I K KE GE +LTW DTK MP+TSRVIQETLRVAS+LSFTFREAVEDVE++GYLIPKG
Sbjct: 306 MAIRKDKEEGE-SLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKG 365

Query: 363 WKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLVL 422
           WKVLPLFRNIHHS +IF  P KFDPSRFEV+ KPNT+MPFGNGTHSCPGNELAKLEM ++
Sbjct: 366 WKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIM 425

Query: 423 LHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLK 460
           +HHLTTKY W++VG  +GIQYGPFALP NGLPI ++ K
Sbjct: 426 IHHLTTKYSWSIVGASDGIQYGPFALPQNGLPIVLARK 462

BLAST of MELO3C016219.jh1 vs. ExPASy Swiss-Prot
Match: Q9FH76 (Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 4.0e-198
Identity = 333/458 (72.71%), Postives = 390/458 (85.15%), Query Frame = 0

Query: 3   VFLSL-VAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASKK 62
           +FL+L  A  FL   R++      ++ KLPLPPG+MG+PY+GET QLYSQDPNVFFA+K+
Sbjct: 6   LFLTLSAAALFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQ 65

Query: 63  KRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDY 122
           +RYG +FK+H+LG PCVM+SSPEA KFVLVTK+HLFKPTFPASKERMLGK AIFFHQGDY
Sbjct: 66  RRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 125

Query: 123 HAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFG 182
           H+KLR+LVLR FMP+AIRN+VP IESIA+ ++ SWDG  +NT+QEMK + F V+L+SI G
Sbjct: 126 HSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSWDGTQLNTYQEMKTYTFNVALISILG 185

Query: 183 KDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKRD 242
           KDE  Y EDLKRCYYILE GYNSMPINLPGTLFHKAMKARKELAQIL  ILS RR+    
Sbjct: 186 KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPSS 245

Query: 243 YDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAEQ 302
           + DLLGSFM +K GLTD QIADN+IG+IFAARDTTASVLTWILKYL +NP++L+AVT EQ
Sbjct: 246 HTDLLGSFMEDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQ 305

Query: 303 ESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKG 362
            +I K K+ GE +LTW DTK MP+T RVIQETLR A++LSFTFREAVEDVE++GYLIPKG
Sbjct: 306 MAIRKDKKEGE-SLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKG 365

Query: 363 WKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLVL 422
           WKVLPLFRNIHH+ +IF  P KFDPSRFEV+ KPNT+MPFG+G HSCPGNELAKLE+ VL
Sbjct: 366 WKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKLEISVL 425

Query: 423 LHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLK 460
           +HHLTTKYRW++VG  +GIQYGPFALP NGLPI +  K
Sbjct: 426 IHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIALERK 462

BLAST of MELO3C016219.jh1 vs. ExPASy Swiss-Prot
Match: Q09J79 (Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A5 PE=2 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 1.3e-185
Identity = 311/438 (71.00%), Postives = 366/438 (83.56%), Query Frame = 0

Query: 23  SAPATRKLPLPPGSMGWPYLGETLQLY-SQDPNVFFASKKKRYGPIFKSHILGYPCVMLS 82
           S  A   LPLPPGSMGWPY+GET QLY S++PNVFF  K+ +YGPIFK+HILG PCVM+S
Sbjct: 30  SEAAACGLPLPPGSMGWPYVGETFQLYSSKNPNVFFNKKRNKYGPIFKTHILGCPCVMVS 89

Query: 83  SPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYHAKLRRLVLRTFMPEAIRNI 142
           SPEA +FVLVT+AHLFKPTFPASKERMLG  AIFF QGDYHA LRR+V R F PE+IR  
Sbjct: 90  SPEAARFVLVTQAHLFKPTFPASKERMLGPQAIFFQQGDYHAHLRRIVSRAFSPESIRAS 149

Query: 143 VPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFGKDEALYFEDLKRCYYILENG 202
           VP+IE+IA  ++ SWDGQ +NTFQEMK +A  V+LLSIFG++E  Y E+LK+CY  LE G
Sbjct: 150 VPAIEAIALRSLHSWDGQFVNTFQEMKTYALNVALLSIFGEEEMRYIEELKQCYLTLEKG 209

Query: 203 YNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKRDYDDLLGSFMGEKEGLTDTQI 262
           YNSMP+NLPGTLFHKAMKARK L  I+  I+S RRE +R  +DLLGSF+  +E LTD QI
Sbjct: 210 YNSMPVNLPGTLFHKAMKARKRLGAIVAHIISARRERQRG-NDLLGSFVDGREALTDAQI 269

Query: 263 ADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAEQESIMKSKENGEDNLTWADTK 322
           ADNVIG+IFAARDTTASVLTW++K+LG++P++L+AVT EQ  I K KE   + L+WADT+
Sbjct: 270 ADNVIGVIFAARDTTASVLTWMVKFLGDHPAVLKAVTEEQLQIAKEKEASGEPLSWADTR 329

Query: 323 NMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKGWKVLPLFRNIHHSPEIFPQP 382
            M +TSRVIQET+RVAS+LSFTFREAVEDVE+ GYLIPKGWKVLPLFRNIHH+P+ FP P
Sbjct: 330 RMKMTSRVIQETMRVASILSFTFREAVEDVEYQGYLIPKGWKVLPLFRNIHHNPDHFPCP 389

Query: 383 EKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHHLTTKYRWNVVGEQEGIQ 442
           EKFDPSRFEV+ KPNT+MPFGNGTHSCPGNELAKLEMLVL HHL TKYRW+    + G+Q
Sbjct: 390 EKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMLVLFHHLATKYRWSTSKSESGVQ 449

Query: 443 YGPFALPLNGLPIRISLK 460
           +GPFALPLNGLP+  + K
Sbjct: 450 FGPFALPLNGLPMSFTRK 466

BLAST of MELO3C016219.jh1 vs. ExPASy Swiss-Prot
Match: Q05JG2 (Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A5 PE=2 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 1.3e-185
Identity = 311/438 (71.00%), Postives = 366/438 (83.56%), Query Frame = 0

Query: 23  SAPATRKLPLPPGSMGWPYLGETLQLY-SQDPNVFFASKKKRYGPIFKSHILGYPCVMLS 82
           S  A   LPLPPGSMGWPY+GET QLY S++PNVFF  K+ +YGPIFK+HILG PCVM+S
Sbjct: 30  SEAAACGLPLPPGSMGWPYVGETFQLYSSKNPNVFFNKKRNKYGPIFKTHILGCPCVMVS 89

Query: 83  SPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYHAKLRRLVLRTFMPEAIRNI 142
           SPEA +FVLVT+AHLFKPTFPASKERMLG  AIFF QGDYHA LRR+V R F PE+IR  
Sbjct: 90  SPEAARFVLVTQAHLFKPTFPASKERMLGPQAIFFQQGDYHAHLRRIVSRAFSPESIRAS 149

Query: 143 VPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFGKDEALYFEDLKRCYYILENG 202
           VP+IE+IA  ++ SWDGQ +NTFQEMK +A  V+LLSIFG++E  Y E+LK+CY  LE G
Sbjct: 150 VPAIEAIALRSLHSWDGQFVNTFQEMKTYALNVALLSIFGEEEMRYIEELKQCYLTLEKG 209

Query: 203 YNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKRDYDDLLGSFMGEKEGLTDTQI 262
           YNSMP+NLPGTLFHKAMKARK L  I+  I+S RRE +R  +DLLGSF+  +E LTD QI
Sbjct: 210 YNSMPVNLPGTLFHKAMKARKRLGAIVAHIISARRERQRG-NDLLGSFVDGREALTDAQI 269

Query: 263 ADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAEQESIMKSKENGEDNLTWADTK 322
           ADNVIG+IFAARDTTASVLTW++K+LG++P++L+AVT EQ  I K KE   + L+WADT+
Sbjct: 270 ADNVIGVIFAARDTTASVLTWMVKFLGDHPAVLKAVTEEQLQIAKEKEASGEPLSWADTR 329

Query: 323 NMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKGWKVLPLFRNIHHSPEIFPQP 382
            M +TSRVIQET+RVAS+LSFTFREAVEDVE+ GYLIPKGWKVLPLFRNIHH+P+ FP P
Sbjct: 330 RMKMTSRVIQETMRVASILSFTFREAVEDVEYQGYLIPKGWKVLPLFRNIHHNPDHFPCP 389

Query: 383 EKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLVLLHHLTTKYRWNVVGEQEGIQ 442
           EKFDPSRFEV+ KPNT+MPFGNGTHSCPGNELAKLEMLVL HHL TKYRW+    + G+Q
Sbjct: 390 EKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMLVLFHHLATKYRWSTSKSESGVQ 449

Query: 443 YGPFALPLNGLPIRISLK 460
           +GPFALPLNGLP+  + K
Sbjct: 450 FGPFALPLNGLPMSFTRK 466

BLAST of MELO3C016219.jh1 vs. ExPASy TrEMBL
Match: A0A1S3BTM7 (abscisic acid 8'-hydroxylase 1 OS=Cucumis melo OX=3656 GN=CYP707A1 PE=3 SV=1)

HSP 1 Score: 925 bits (2391), Expect = 0.0
Identity = 460/460 (100.00%), Postives = 460/460 (100.00%), Query Frame = 0

Query: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60
           MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK
Sbjct: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240
           GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR
Sbjct: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240

Query: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300
           DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE
Sbjct: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300

Query: 301 QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460
           LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK
Sbjct: 421 LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460

BLAST of MELO3C016219.jh1 vs. ExPASy TrEMBL
Match: A0A0A0KX97 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G056600 PE=3 SV=1)

HSP 1 Score: 905 bits (2338), Expect = 0.0
Identity = 447/460 (97.17%), Postives = 456/460 (99.13%), Query Frame = 0

Query: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60
           MYVF+SL+AFFFLLFF YLFKFSAP+ RKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK
Sbjct: 1   MYVFVSLLAFFFLLFFHYLFKFSAPSRRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240
           GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELA+ILNKILSTRRE KR
Sbjct: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAEILNKILSTRRETKR 240

Query: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300
           +YDDLLGSFMGEKEGLTD QIADNVIGLIFAARDTTASVLTWI+KYLGENPSILQAVTAE
Sbjct: 241 EYDDLLGSFMGEKEGLTDKQIADNVIGLIFAARDTTASVLTWIVKYLGENPSILQAVTAE 300

Query: 301 QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QESI+KSKENG+DNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QESIVKSKENGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460
           LLHHLTTK+RWNVVGEQEGIQYGPFALPLNGLPIRISLKK
Sbjct: 421 LLHHLTTKFRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460

BLAST of MELO3C016219.jh1 vs. ExPASy TrEMBL
Match: A0A6J1EY25 (abscisic acid 8'-hydroxylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111437516 PE=3 SV=1)

HSP 1 Score: 860 bits (2223), Expect = 2.51e-314
Identity = 423/460 (91.96%), Postives = 441/460 (95.87%), Query Frame = 0

Query: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60
           M+ F +++A FFLL   +LFKFSAPA RKLPLPPGSMGWPY+GETLQLYSQDPNVFFASK
Sbjct: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVM+SSPEA KFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240
           GK+EALYFEDLKRCYY LE GYNSMPINLPGTLFHKAMKARKELA IL KILSTRREMKR
Sbjct: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240

Query: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300
           D++DLLGSFMGEKEGLTD QIADNVIGLIFAARDTTASVLTWILKYLGENPS+LQAVT E
Sbjct: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTDE 300

Query: 301 QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QE+IMK K++G+DNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QEAIMKEKQSGDDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPE FPQP+KFDPSRFEV QKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460
           LLHHLTTKYRW+VVG QEGIQYGPFALP NGLPI+ISLKK
Sbjct: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 460

BLAST of MELO3C016219.jh1 vs. ExPASy TrEMBL
Match: A0A6J1J3P6 (abscisic acid 8'-hydroxylase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483101 PE=3 SV=1)

HSP 1 Score: 860 bits (2222), Expect = 3.56e-314
Identity = 422/460 (91.74%), Postives = 442/460 (96.09%), Query Frame = 0

Query: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60
           M+ F +++A FFLL   +LFKFSAPA RKLPLPPGSMGWPY+GETLQLYSQDPNVFFASK
Sbjct: 1   MFCFAAVIAAFFLLLLHFLFKFSAPARRKLPLPPGSMGWPYMGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVM+SSPEA KFVLVTKAHLFKPTFPASKERMLGKNAIFFH+GD
Sbjct: 61  KKRYGPIFKSHILGYPCVMVSSPEAAKFVLVTKAHLFKPTFPASKERMLGKNAIFFHRGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240
           GK+EALYFEDLKRCYY LE GYNSMPINLPGTLFHKAMKARKELA IL KILSTRREMKR
Sbjct: 181 GKEEALYFEDLKRCYYTLEKGYNSMPINLPGTLFHKAMKARKELAHILTKILSTRREMKR 240

Query: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300
           D++DLLGSFMGEKEGLTD QIADNVIGLIFAARDTTASVLTWILKYLGENPS+LQAVTAE
Sbjct: 241 DHNDLLGSFMGEKEGLTDEQIADNVIGLIFAARDTTASVLTWILKYLGENPSVLQAVTAE 300

Query: 301 QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QE+IMK K++G+DNLTW+DTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QEAIMKEKQSGDDNLTWSDTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWKVLPLFRNIHHSPE FPQP+KFDPSRFEV QKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWKVLPLFRNIHHSPENFPQPDKFDPSRFEVPQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460
           LLHHLTTKYRW+VVG QEGIQYGPFALP NGLPI+ISLKK
Sbjct: 421 LLHHLTTKYRWSVVGAQEGIQYGPFALPHNGLPIKISLKK 460

BLAST of MELO3C016219.jh1 vs. ExPASy TrEMBL
Match: A0A5D3CZR9 (Abscisic acid 8'-hydroxylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G002520 PE=3 SV=1)

HSP 1 Score: 854 bits (2207), Expect = 2.49e-312
Identity = 433/460 (94.13%), Postives = 433/460 (94.13%), Query Frame = 0

Query: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60
           MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK
Sbjct: 1   MYVFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASK 60

Query: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120
           KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD
Sbjct: 61  KKRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGD 120

Query: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180
           YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF
Sbjct: 121 YHAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIF 180

Query: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240
           GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR
Sbjct: 181 GKDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 240

Query: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300
           DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE
Sbjct: 241 DYDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAE 300

Query: 301 QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360
           QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK
Sbjct: 301 QESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPK 360

Query: 361 GWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420
           GWK                           VSQKPNTYMPFGNGTHSCPGNELAKLEMLV
Sbjct: 361 GWK---------------------------VSQKPNTYMPFGNGTHSCPGNELAKLEMLV 420

Query: 421 LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 460
           LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK
Sbjct: 421 LLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLKK 433

BLAST of MELO3C016219.jh1 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 713.8 bits (1841), Expect = 9.1e-206
Identity = 343/458 (74.89%), Postives = 399/458 (87.12%), Query Frame = 0

Query: 3   VFLSLVA-FFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASKK 62
           +FL+L A   FL F R L       + KLPLPPG+MGWPY+GET QLYSQDPNVFF SK+
Sbjct: 6   LFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQ 65

Query: 63  KRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDY 122
           KRYG +FK+H+LG PCVM+SSPEA KFVLVTK+HLFKPTFPASKERMLGK AIFFHQGDY
Sbjct: 66  KRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 125

Query: 123 HAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFG 182
           HAKLR+LVLR FMPE+IRN+VP IESIA+++++SW+G +INT+QEMK + F V+LLSIFG
Sbjct: 126 HAKLRKLVLRAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFG 185

Query: 183 KDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKRD 242
           KDE LY EDLKRCYYILE GYNSMP+NLPGTLFHK+MKARKEL+QIL +ILS RR+    
Sbjct: 186 KDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSS 245

Query: 243 YDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAEQ 302
           ++DLLGSFMG+KE LTD QIADN+IG+IFAARDTTASV++WILKYL ENP++L+AVT EQ
Sbjct: 246 HNDLLGSFMGDKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQ 305

Query: 303 ESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKG 362
            +I K KE GE +LTW DTK MP+TSRVIQETLRVAS+LSFTFREAVEDVE++GYLIPKG
Sbjct: 306 MAIRKDKEEGE-SLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKG 365

Query: 363 WKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLVL 422
           WKVLPLFRNIHHS +IF  P KFDPSRFEV+ KPNT+MPFGNGTHSCPGNELAKLEM ++
Sbjct: 366 WKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIM 425

Query: 423 LHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLK 460
           +HHLTTKY W++VG  +GIQYGPFALP NGLPI ++ K
Sbjct: 426 IHHLTTKYSWSIVGASDGIQYGPFALPQNGLPIVLARK 462

BLAST of MELO3C016219.jh1 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 692.2 bits (1785), Expect = 2.8e-199
Identity = 333/458 (72.71%), Postives = 390/458 (85.15%), Query Frame = 0

Query: 3   VFLSL-VAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASKK 62
           +FL+L  A  FL   R++      ++ KLPLPPG+MG+PY+GET QLYSQDPNVFFA+K+
Sbjct: 6   LFLTLSAAALFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQ 65

Query: 63  KRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDY 122
           +RYG +FK+H+LG PCVM+SSPEA KFVLVTK+HLFKPTFPASKERMLGK AIFFHQGDY
Sbjct: 66  RRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 125

Query: 123 HAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFG 182
           H+KLR+LVLR FMP+AIRN+VP IESIA+ ++ SWDG  +NT+QEMK + F V+L+SI G
Sbjct: 126 HSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSWDGTQLNTYQEMKTYTFNVALISILG 185

Query: 183 KDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKRD 242
           KDE  Y EDLKRCYYILE GYNSMPINLPGTLFHKAMKARKELAQIL  ILS RR+    
Sbjct: 186 KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPSS 245

Query: 243 YDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAEQ 302
           + DLLGSFM +K GLTD QIADN+IG+IFAARDTTASVLTWILKYL +NP++L+AVT EQ
Sbjct: 246 HTDLLGSFMEDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQ 305

Query: 303 ESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKG 362
            +I K K+ GE +LTW DTK MP+T RVIQETLR A++LSFTFREAVEDVE++GYLIPKG
Sbjct: 306 MAIRKDKKEGE-SLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKG 365

Query: 363 WKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLVL 422
           WKVLPLFRNIHH+ +IF  P KFDPSRFEV+ KPNT+MPFG+G HSCPGNELAKLE+ VL
Sbjct: 366 WKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKLEISVL 425

Query: 423 LHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRISLK 460
           +HHLTTKYRW++VG  +GIQYGPFALP NGLPI +  K
Sbjct: 426 IHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIALERK 462

BLAST of MELO3C016219.jh1 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 676.0 bits (1743), Expect = 2.1e-194
Identity = 330/454 (72.69%), Postives = 385/454 (84.80%), Query Frame = 0

Query: 3   VFLSLVA-FFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASKK 62
           +FL+L A   FL F R L       + KLPLPPG+MGWPY+GET QLYSQDPNVFF SK+
Sbjct: 6   LFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQ 65

Query: 63  KRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDY 122
           KRYG +FK+H+LG PCVM+SSPEA KFVLVTK+HLFKPTFPASKERMLGK AIFFHQGDY
Sbjct: 66  KRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 125

Query: 123 HAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFG 182
           HAKLR+LVLR FMPE+IRN+VP IESIA+++++SW+G +INT+QEMK + F V+LLSIFG
Sbjct: 126 HAKLRKLVLRAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFG 185

Query: 183 KDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKRD 242
           KDE LY EDLKRCYYILE GYNSMP+NLPGTLFHK+MKARKEL+QIL +ILS RR+    
Sbjct: 186 KDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSS 245

Query: 243 YDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAEQ 302
           ++DLLGSFMG+KE LTD QIADN+IG+IFAARDTTASV++WILKYL ENP++L+AVT EQ
Sbjct: 246 HNDLLGSFMGDKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQ 305

Query: 303 ESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKG 362
            +I K KE GE +LTW DTK MP+TSRVIQETLRVAS+LSFTFREAVEDVE++GYLIPKG
Sbjct: 306 MAIRKDKEEGE-SLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKG 365

Query: 363 WKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLVL 422
           WKVLPLFRNIHHS +IF  P KFDPSRFEV+ KPNT+MPFGNGTHSCPGNELAKLEM ++
Sbjct: 366 WKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIM 425

Query: 423 LHHLTTKYRWNVVGEQ-EGIQYGPFALPLNGLPI 455
           +HHLTTKYR+  + E+  G   G    P    P+
Sbjct: 426 IHHLTTKYRFGQLLERATGFSMGHSRFPKTDCPL 458

BLAST of MELO3C016219.jh1 vs. TAIR 10
Match: AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 647.1 bits (1668), Expect = 1.0e-185
Identity = 314/429 (73.19%), Postives = 367/429 (85.55%), Query Frame = 0

Query: 3   VFLSL-VAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASKK 62
           +FL+L  A  FL   R++      ++ KLPLPPG+MG+PY+GET QLYSQDPNVFFA+K+
Sbjct: 6   LFLTLSAAALFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQ 65

Query: 63  KRYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDY 122
           +RYG +FK+H+LG PCVM+SSPEA KFVLVTK+HLFKPTFPASKERMLGK AIFFHQGDY
Sbjct: 66  RRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 125

Query: 123 HAKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQLINTFQEMKMFAFEVSLLSIFG 182
           H+KLR+LVLR FMP+AIRN+VP IESIA+ ++ SWDG  +NT+QEMK + F V+L+SI G
Sbjct: 126 HSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSWDGTQLNTYQEMKTYTFNVALISILG 185

Query: 183 KDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKRD 242
           KDE  Y EDLKRCYYILE GYNSMPINLPGTLFHKAMKARKELAQIL  ILS RR+    
Sbjct: 186 KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPSS 245

Query: 243 YDDLLGSFMGEKEGLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQAVTAEQ 302
           + DLLGSFM +K GLTD QIADN+IG+IFAARDTTASVLTWILKYL +NP++L+AVT EQ
Sbjct: 246 HTDLLGSFMEDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQ 305

Query: 303 ESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGYLIPKG 362
            +I K K+ GE +LTW DTK MP+T RVIQETLR A++LSFTFREAVEDVE++GYLIPKG
Sbjct: 306 MAIRKDKKEGE-SLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKG 365

Query: 363 WKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKLEMLVL 422
           WKVLPLFRNIHH+ +IF  P KFDPSRFEV+ KPNT+MPFG+G HSCPGNELAKLE+ VL
Sbjct: 366 WKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKLEISVL 425

Query: 423 LHHLTTKYR 431
           +HHLTTKYR
Sbjct: 426 IHHLTTKYR 433

BLAST of MELO3C016219.jh1 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 572.8 bits (1475), Expect = 2.5e-163
Identity = 279/461 (60.52%), Postives = 358/461 (77.66%), Query Frame = 0

Query: 3   VFLSLVAFFFLLFFRYLFKFSAPATRKLPLPPGSMGWPYLGETLQLYSQDPNVFFASKKK 62
           + L++V    +L F++   +     ++L LPPGSMG PY+GETL+LY+++PN FFA+++ 
Sbjct: 23  ITLTIVVVVVVLLFKWWLHWK---EQRLRLPPGSMGLPYIGETLRLYTENPNSFFATRQN 82

Query: 63  RYGPIFKSHILGYPCVMLSSPEAVKFVLVTKAHLFKPTFPASKERMLGKNAIFFHQGDYH 122
           +YG IFK+HILG PCVM+SSPEA + VLV+KAHLFKPT+P SKERM+G  A+FFHQG YH
Sbjct: 83  KYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYH 142

Query: 123 AKLRRLVLRTFMPEAIRNIVPSIESIAKNTVQSWDGQ-LINTFQEMKMFAFEVSLLSIFG 182
           + L+RLV  +FMP A+R  V  IE +   T+ SW  Q  INT + MK +AF+V+++S FG
Sbjct: 143 STLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAFG 202

Query: 183 -KDEALYFEDLKRCYYILENGYNSMPINLPGTLFHKAMKARKELAQILNKILSTRREMKR 242
            K+E    + +K  Y  LE GYNSMP++LPGTLFHK+MKAR EL++ L K++  RRE  R
Sbjct: 203 DKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGR 262

Query: 243 DYDDLLGSFMGEKE----GLTDTQIADNVIGLIFAARDTTASVLTWILKYLGENPSILQA 302
           +   LLG  +G K+    GL+D+QIADN+IG+IFAA DTTASVLTW+LKYL ++P++LQ 
Sbjct: 263 EEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQE 322

Query: 303 VTAEQESIMKSKENGEDNLTWADTKNMPITSRVIQETLRVASVLSFTFREAVEDVEFDGY 362
           V+ EQ SI +  +     ++W DT+ MP+T+RVIQETLR ASVLSFTFREAV+DVE+DGY
Sbjct: 323 VSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGY 382

Query: 363 LIPKGWKVLPLFRNIHHSPEIFPQPEKFDPSRFEVSQKPNTYMPFGNGTHSCPGNELAKL 422
           LIPKGWKVLPLFR IHHS E FP PEKFDPSRFEV+ KP TYMPFGNG HSCPG+ELAKL
Sbjct: 383 LIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKL 442

Query: 423 EMLVLLHHLTTKYRWNVVGEQEGIQYGPFALPLNGLPIRIS 458
           EML+LLHHLTT +RW V+G++EGIQYGPF +P  GLPIR++
Sbjct: 443 EMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVT 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008451873.10.0100.00PREDICTED: abscisic acid 8'-hydroxylase 1 [Cucumis melo][more]
XP_004150544.20.097.17abscisic acid 8'-hydroxylase CYP707A2 [Cucumis sativus] >KGN53469.1 hypothetical... [more]
XP_038877343.10.094.30abscisic acid 8'-hydroxylase CYP707A2-like [Benincasa hispida][more]
XP_022931288.15.18e-31491.96abscisic acid 8'-hydroxylase 1-like [Cucurbita moschata] >XP_023553532.1 abscisi... [more]
XP_022985007.17.35e-31491.74abscisic acid 8'-hydroxylase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
K4CI529.5e-20876.57Abscisic acid 8'-hydroxylase CYP707A2 OS=Solanum lycopersicum OX=4081 GN=CYP707A... [more]
Q949P11.3e-20474.89Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 ... [more]
Q9FH764.0e-19872.71Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 ... [more]
Q09J791.3e-18571.00Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
Q05JG21.3e-18571.00Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70... [more]
Match NameE-valueIdentityDescription
A0A1S3BTM70.0100.00abscisic acid 8'-hydroxylase 1 OS=Cucumis melo OX=3656 GN=CYP707A1 PE=3 SV=1[more]
A0A0A0KX970.097.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G056600 PE=3 SV=1[more]
A0A6J1EY252.51e-31491.96abscisic acid 8'-hydroxylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11143751... [more]
A0A6J1J3P63.56e-31491.74abscisic acid 8'-hydroxylase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483101 ... [more]
A0A5D3CZR92.49e-31294.13Abscisic acid 8'-hydroxylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
Match NameE-valueIdentityDescription
AT4G19230.19.1e-20674.89cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT5G45340.12.8e-19972.71cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT4G19230.22.1e-19472.69cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT5G45340.21.0e-18573.19cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT2G29090.12.5e-16360.52cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 217..237
NoneNo IPR availablePIRSRPIRSR000209-1PIRSR000209-1coord: 30..422
e-value: 6.1E-33
score: 111.4
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 10..457
NoneNo IPR availablePANTHERPTHR24286:SF249ABSCISIC ACID 8'-HYDROXYLASE CYP707A2coord: 10..457
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 398..408
score: 44.06
coord: 367..391
score: 29.22
coord: 260..277
score: 26.45
coord: 61..80
score: 31.57
coord: 408..431
score: 26.83
coord: 327..345
score: 26.01
coord: 280..306
score: 22.79
IPR001128Cytochrome P450PRINTSPR00385P450coord: 328..339
score: 37.55
coord: 399..408
score: 47.36
coord: 271..288
score: 35.59
coord: 408..419
score: 40.5
IPR001128Cytochrome P450PFAMPF00067p450coord: 33..437
e-value: 1.5E-81
score: 274.5
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 23..458
e-value: 1.2E-122
score: 411.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 28..456
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 401..410

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C016219.jh1.t1MELO3C016219.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen