MELO3C014733.jh1 (gene) Melon (Harukei-3) v1.41

BLAST of MELO3C014733.jh1 vs. NCBI nr
Match: XP_008449904.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008449905.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0040122.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK21744.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 735 bits (1897), Expect = 1.64e-267
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
           QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR
Sbjct: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360
           AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ
Sbjct: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360

Query: 361 RDHTSI 366
           RDHTSI
Sbjct: 361 RDHTSI 366

BLAST of MELO3C014733.jh1 vs. NCBI nr
Match: XP_031739817.1 (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SIRP1 [Cucumis sativus])

HSP 1 Score: 704 bits (1817), Expect = 2.13e-255
Identity = 357/366 (97.54%), Postives = 357/366 (97.54%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGN VSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAGIATAESENSYE ERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYE-ERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
           QNQN NSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDS 
Sbjct: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360
           AARFMSDLN    NGNGNGSGEIE RNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ
Sbjct: 301 AARFMSDLN----NGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360

Query: 361 RDHTSI 366
           RDHTSI
Sbjct: 361 RDHTSI 361

BLAST of MELO3C014733.jh1 vs. NCBI nr
Match: XP_038901761.1 (E3 ubiquitin-protein ligase SIRP1 [Benincasa hispida] >XP_038901762.1 E3 ubiquitin-protein ligase SIRP1 [Benincasa hispida])

HSP 1 Score: 638 bits (1645), Expect = 2.87e-229
Identity = 328/367 (89.37%), Postives = 341/367 (92.92%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGIS+DNNDNN+NNN   EL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISNDNNDNNSNNN---EL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDNEHEDGEGN VSSE T+LD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNEHEDGEGNRVSSEPTDLD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAG+ATAESENSYEGERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
           QNQN   IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDALPTVRVE   DS 
Sbjct: 181 QNQN--PIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVE-PVDSC 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQC+VCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD DGGS
Sbjct: 241 LQCAVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDADGGS 300

Query: 301 AARFMSDLNNGNGNGNGNGSGEI-EERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDS 360
             RFM DLN    N NG+GSGEI + RN+S +GRRFSFPWPFNGLF++QGNSS GSASDS
Sbjct: 301 T-RFMGDLN----NANGSGSGEIIDGRNASGTGRRFSFPWPFNGLFSAQGNSSGGSASDS 356

Query: 361 QRDHTSI 366
           QRDH SI
Sbjct: 361 QRDHASI 356

BLAST of MELO3C014733.jh1 vs. NCBI nr
Match: KAE8649424.1 (hypothetical protein Csa_021650 [Cucumis sativus])

HSP 1 Score: 622 bits (1604), Expect = 1.60e-223
Identity = 321/366 (87.70%), Postives = 321/366 (87.70%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGN VSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQ                                     VQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQ-------------------------------------VQGGEN 180

Query: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
           QNQN NSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDS 
Sbjct: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360
           AARFMSDLN    NGNGNGSGEIE RNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ
Sbjct: 301 AARFMSDLN----NGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 325

Query: 361 RDHTSI 366
           RDHTSI
Sbjct: 361 RDHTSI 325

BLAST of MELO3C014733.jh1 vs. NCBI nr
Match: XP_022944289.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata])

HSP 1 Score: 618 bits (1593), Expect = 3.88e-221
Identity = 321/378 (84.92%), Postives = 342/378 (90.48%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDE IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEE+GNG+SSDNN NNNNNN+NN L
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNHVSSETT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDN   EH DGEGN VSSE T
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVIRRRR-SSATILQLLQGIRAGIATAESENSYEGERSRERER--------VILINP 180
           ELDSVIRRRR SSATILQLLQGIRAG+AT ESENSYEGERSRERER        VILINP
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180

Query: 181 FNQTIVVQGGENQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDAL 240
           FNQTIVVQGGE  +QNHN+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDAL
Sbjct: 181 FNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP 300
           PTVRVE   D+RLQCSVCLDEFE+DEEAKEMPC+HKFH+GCILPWLELHSSCPVCRHQLP
Sbjct: 241 PTVRVECG-DNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLP 300

Query: 301 GDESKRDVDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQ 360
            DESKRD DGGSA RFM     G+G+G+G+GSGEI+ RN+S +GRRFSFPWPFNGLF+SQ
Sbjct: 301 ADESKRDADGGSA-RFM-----GSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQ 360

Query: 361 GNSSAGSASDSQRDHTSI 366
           GNSS GS S+SQRDHTSI
Sbjct: 361 GNSSGGSGSESQRDHTSI 369

BLAST of MELO3C014733.jh1 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 9.0e-52
Identity = 143/375 (38.13%), Postives = 191/375 (50.93%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           M E   TRYWCH C Q +   +  EIKCP C  GFVEE+         D       N + 
Sbjct: 1   MAEAAITRYWCHECEQAIEEAMVDEIKCPSCGGGFVEEM--------TDEEIERLTNRQP 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120
           E  F      S W PI               H     D DD +++ G          E +
Sbjct: 61  EPGF------SQWNPI--------------EHPGETMDSDDEDNDLGR---------EFE 120

Query: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGG-- 180
             IRR R ++T+ ++L  I   +A             +ER+  ILIN FNQ + +QG   
Sbjct: 121 GFIRRHRRASTLRRVLDSIHDDLAD-----------DQERDSSILINAFNQALALQGSVL 180

Query: 181 ------ENQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVR 240
                  +Q  + N  G L +Y +G GL LLLQH+AE+DP+R GTPPA+KEAV+ALPTV+
Sbjct: 181 DPDEGQGDQGGSTNDDGLLEEYVLGAGLSLLLQHLAESDPSRNGTPPAKKEAVEALPTVK 240

Query: 241 VELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDES 300
           +E      + CSVCLD+ EV  +AK+MPC+HKFH+ CILPWLELHSSCPVCR +LP +E+
Sbjct: 241 IE----EVVSCSVCLDDLEVGSQAKQMPCEHKFHSSCILPWLELHSSCPVCRFELPSEET 300

Query: 301 KRDVDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSF-PWPFNGLF-TSQGN 360
           K   +  +  R          +G GN         S  S R ++  PW  N LF T +  
Sbjct: 301 KDLNEPSNIGRVEDSHEEVRADGPGN--------VSESSNRPWAIVPW-LNELFSTREAQ 314

Query: 361 SSAGSASDSQRDHTS 366
           ++ G ++D Q  HTS
Sbjct: 361 NAGGVSTDQQSPHTS 314

BLAST of MELO3C014733.jh1 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.8e-36
Identity = 80/177 (45.20%), Postives = 107/177 (60.45%), Query Frame = 0

Query: 190 SLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELE--EDSRLQCSVCL 249
           ++GDYF+GPGL+ L+Q +AENDPNRYGTPPA K A++ALP V +          QC+VC+
Sbjct: 169 NIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCM 228

Query: 250 DEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDES--KRDVDGGSAARFM 309
           D+FE   EAK+MPCKH +H  C+LPWLELH+SCPVCRH+LP D+   +R V G       
Sbjct: 229 DDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQG---- 288

Query: 310 SDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRD 363
               +G  +G+ +G     +  + E   R S PWPF       G +   +A   Q D
Sbjct: 289 ---TSGGNDGDNSGQRSDGDNRTVERSFRISLPWPFQA--RGPGPAPGDNAETRQED 336

BLAST of MELO3C014733.jh1 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 5.3e-36
Identity = 118/357 (33.05%), Postives = 163/357 (45.66%), Query Frame = 0

Query: 9   YWCHMCSQMVNPIV--DVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNELESDFGS 68
           ++C+ C+Q V   +    +  CP C  GF+EE       D N N + N N N  +S F  
Sbjct: 21  FFCYQCNQTVTISISSSADPFCPICNQGFLEEY-----EDPNPNQSLNFNPNSSDSFFPM 80

Query: 69  ERALSLWAPILLG-MMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELDSVIRR 128
               S   P++ G     P G                  +    +      T L + ++ 
Sbjct: 81  ADPFSTLLPLIFGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFTFLQNHLQT 140

Query: 129 RRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGENQNQNH 188
            RSS T  + +          E+  S  G R                             
Sbjct: 141 LRSSGTHFEFV---------IENHPSDPGNRMP--------------------------- 200

Query: 189 NSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRV--ELEEDSRLQC 248
              G+ GDYF GPGL+ L+Q +AENDPNRYGTPPA K A+DALPTV+V  ++ +    QC
Sbjct: 201 ---GNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQC 260

Query: 249 SVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAAR 308
           +VC+DEFE   + K+MPCKH FH  C+LPWLELH+SCPVCR +LP D+   +        
Sbjct: 261 AVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYE-------- 312

Query: 309 FMSDLNNGNGN-GNGNGSGEIEERNSSESGRRFS--FPWPFNGLFTSQGNSSAGSAS 358
                N   G+ G+G+G G +E + +     RFS   PWPF      Q  S +GS +
Sbjct: 321 -----NRSQGSQGSGDGQGSVEGQQTP----RFSIQLPWPFR----RQDGSGSGSGA 312

BLAST of MELO3C014733.jh1 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 3.2e-25
Identity = 70/173 (40.46%), Postives = 92/173 (53.18%), Query Frame = 0

Query: 189 GSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELE--EDSRLQCSVC 248
           G+ GDYF GPGL+ L + ++     R G PPA + A+DALPT+++       S   C VC
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTTRR-GPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 249 LDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMS 308
            DEFE+  EAK+MPC H +H+ CI+PWL  H+SCPVCR +LP           SA+   S
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELP-----------SASGPSS 254

Query: 309 DLNNGNGNGNGNGSGEIEERNSSESGRR----FSFPWPFNGLFTSQGNSSAGS 356
             N      N   S      NS E+G      FS  WPF     S G+SS+ +
Sbjct: 255 SQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFR----SSGSSSSST 291

BLAST of MELO3C014733.jh1 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.6e-24
Identity = 66/176 (37.50%), Postives = 95/176 (53.98%), Query Frame = 0

Query: 192 GDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELE--EDSRLQCSVCLDE 251
           GDYF GPGL+ L++ ++    +R G PPA K ++DALPT+++  +  + S   C VC DE
Sbjct: 134 GDYFFGPGLEELIEQLSSGTHHR-GPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDE 193

Query: 252 FEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMSDLN 311
           FE+  EAK+MPC H +H+ CI+PWL  H+SCPVCR +LP                     
Sbjct: 194 FELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELP--------------------- 253

Query: 312 NGNGNGNGNGSGEIEERNSSESGRR---FSFPWPFNGLFTSQGNSSAGSASDSQRD 363
              G+ +   S +    N  E+ RR   FS  WPF         SS+ S++ ++RD
Sbjct: 254 -SRGSSSSTQSSQNRSTNGRENSRRRNIFSNLWPF--------RSSSSSSTQNRRD 278

BLAST of MELO3C014733.jh1 vs. ExPASy TrEMBL
Match: A0A1S3BNR7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 SV=1)

HSP 1 Score: 735 bits (1897), Expect = 7.94e-268
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
           QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR
Sbjct: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360
           AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ
Sbjct: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360

Query: 361 RDHTSI 366
           RDHTSI
Sbjct: 361 RDHTSI 366

BLAST of MELO3C014733.jh1 vs. ExPASy TrEMBL
Match: A0A5D3DDU3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001160 PE=4 SV=1)

HSP 1 Score: 735 bits (1897), Expect = 7.94e-268
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
           QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR
Sbjct: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360
           AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ
Sbjct: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360

Query: 361 RDHTSI 366
           RDHTSI
Sbjct: 361 RDHTSI 366

BLAST of MELO3C014733.jh1 vs. ExPASy TrEMBL
Match: A0A0A0KWR3 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G267990 PE=4 SV=1)

HSP 1 Score: 711 bits (1835), Expect = 1.93e-258
Identity = 358/366 (97.81%), Postives = 358/366 (97.81%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELD 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGN VSSETTELD
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELD 120

Query: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180
           SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN
Sbjct: 121 SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGEN 180

Query: 181 QNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSR 240
           QNQN NSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDS 
Sbjct: 181 QNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSC 240

Query: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300
           LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS
Sbjct: 241 LQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGS 300

Query: 301 AARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360
           AARFMSDLN    NGNGNGSGEIE RNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ
Sbjct: 301 AARFMSDLN----NGNGNGSGEIEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQ 360

Query: 361 RDHTSI 366
           RDHTSI
Sbjct: 361 RDHTSI 362

BLAST of MELO3C014733.jh1 vs. ExPASy TrEMBL
Match: A0A6J1FWF9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781 PE=4 SV=1)

HSP 1 Score: 618 bits (1593), Expect = 1.88e-221
Identity = 321/378 (84.92%), Postives = 342/378 (90.48%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDE IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEE+GNG+SSDNN NNNNNN+NN L
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNHVSSETT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDDDN   EH DGEGN VSSE T
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDSVIRRRR-SSATILQLLQGIRAGIATAESENSYEGERSRERER--------VILINP 180
           ELDSVIRRRR SSATILQLLQGIRAG+AT ESENSYEGERSRERER        VILINP
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVILINP 180

Query: 181 FNQTIVVQGGENQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDAL 240
           FNQTIVVQGGE  +QNHN+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVDAL
Sbjct: 181 FNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP 300
           PTVRVE   D+RLQCSVCLDEFE+DEEAKEMPC+HKFH+GCILPWLELHSSCPVCRHQLP
Sbjct: 241 PTVRVECG-DNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLP 300

Query: 301 GDESKRDVDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFTSQ 360
            DESKRD DGGSA RFM     G+G+G+G+GSGEI+ RN+S +GRRFSFPWPFNGLF+SQ
Sbjct: 301 ADESKRDADGGSA-RFM-----GSGSGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQ 360

Query: 361 GNSSAGSASDSQRDHTSI 366
           GNSS GS S+SQRDHTSI
Sbjct: 361 GNSSGGSGSESQRDHTSI 369

BLAST of MELO3C014733.jh1 vs. ExPASy TrEMBL
Match: A0A6J1JDY5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 PE=4 SV=1)

HSP 1 Score: 608 bits (1568), Expect = 1.50e-217
Identity = 317/380 (83.42%), Postives = 339/380 (89.21%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           MDE IATRYWCH+CSQMVNPIVDVEIKCPFCRSGFVEE+GNG+SSDNN NNNNN+NN  L
Sbjct: 1   MDESIATRYWCHVCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNSNN-AL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN---EHEDGEGNHVSSETT 120
           ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFD+DDD+N   EH DGEGN  SSE  
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPA 120

Query: 121 ELDSVIRRRR-SSATILQLLQGIRAGIATAESENSYEGERSRERER----------VILI 180
           ELDSVIRRRR SSATILQLLQGIRAG+AT ESENSYEGERSRERER          VILI
Sbjct: 121 ELDSVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERERVILI 180

Query: 181 NPFNQTIVVQGGENQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVD 240
           NPFNQTIVVQGGE  +QNHN+IGSLGDYFVGPGLDLLLQH+AENDPNRYGTPPAQKEAVD
Sbjct: 181 NPFNQTIVVQGGE--SQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVD 240

Query: 241 ALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQ 300
           ALPTVRVE   D+RLQCSVCLDEFE+DEEAKEMPCKHKFH+GCILPWLELHSSCPVCRHQ
Sbjct: 241 ALPTVRVECG-DNRLQCSVCLDEFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQ 300

Query: 301 LPGDESKRDVDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPFNGLFT 360
           LP DESKRD DGGSA RFM +L+NGN +G+G+GSGEI+ R +S  GRRFSFPWPFNGLF+
Sbjct: 301 LPADESKRDTDGGSA-RFMGELSNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFS 360

Query: 361 SQGNSSAGSASDSQRDHTSI 366
           SQGNSS GS S+SQRDHT I
Sbjct: 361 SQGNSSGGSESESQRDHTPI 375

BLAST of MELO3C014733.jh1 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 379.4 bits (973), Expect = 3.2e-105
Identity = 215/392 (54.85%), Postives = 268/392 (68.37%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEI-GNGISSDNNDNNNNNNNNNE 60
           M+E +A RYWCHMCSQMVNP+++ EIKCPFC+SGF+EE+ GNG             +  +
Sbjct: 1   MEETMAARYWCHMCSQMVNPVMESEIKCPFCQSGFIEEMSGNG----GGGGGRGIRDVQD 60

Query: 61  LESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDN-----EHEDGEGNHVSS 120
            E+DFG++RALSLWAPILLGMM +PR RRRFR  EF +++DDN      + DG GN  ++
Sbjct: 61  SETDFGTDRALSLWAPILLGMMSSPRRRRRFRRSEFGEENDDNGDDLTTNADGNGNDSNN 120

Query: 121 ETT-------------------ELDSVI-RRRRSSATILQLLQGIRAGIATAESENSYEG 180
                                 E +S++ RRRRSS  ILQLLQGIRAGIA+    +    
Sbjct: 121 SNNNVYRHHRARRHGGEIDLDREFESILRRRRRSSGNILQLLQGIRAGIASEYESSDNNW 180

Query: 181 ERSRERERVILINPFNQTIVVQGGENQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNR 240
           + SRER+RVI+INP+NQ++VV    +QNQNH S+ SLGDYF+GPGLDLLLQH+AENDPNR
Sbjct: 181 DNSRERDRVIMINPYNQSLVVP--SDQNQNHPSLTSLGDYFIGPGLDLLLQHLAENDPNR 240

Query: 241 YGTPPAQKEAVDALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWL 300
            GTPPA+KEAV+ALPTV++       LQCSVCLD+FE   EAKEMPCKHKFH  CI+PWL
Sbjct: 241 QGTPPARKEAVEALPTVKIM----EPLQCSVCLDDFEKGTEAKEMPCKHKFHVRCIVPWL 300

Query: 301 ELHSSCPVCRHQLPG-----DESKRDVDGGSAARFMSDLNNGNGNGN-GNGSGEIEERNS 360
           ELHSSCPVCR +LP      DE+K D +     R + + +NGN   N GN     E+   
Sbjct: 301 ELHSSCPVCRFELPSSADDDDETKTDSERVLRTRNVRETSNGNVVENVGNADRGREDEVR 360

BLAST of MELO3C014733.jh1 vs. TAIR 10
Match: AT4G26400.2 (RING/U-box superfamily protein )

HSP 1 Score: 319.7 bits (818), Expect = 3.1e-87
Identity = 192/384 (50.00%), Postives = 249/384 (64.84%), Query Frame = 0

Query: 5   IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNELESDF 64
           ++ RYWCHMCSQMVNP++  EIKCPFC+SGFVEE+   I   N  ++++     + E DF
Sbjct: 1   MSARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEEMSREI---NGGSSSSLREVQDPEIDF 60

Query: 65  GSERALSLWAPILLGMMGNPRGRRRFRHLEFD-DDDDDNEHEDGEGN------------H 124
           G++RALSLW PILLGMM NPR RRRFR  EF  D+D+ N  +D +GN            H
Sbjct: 61  GTDRALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRH 120

Query: 125 VSSE-------TTELDSVI-RRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERV 184
              +         E +S++ RRRRSSATILQLLQGIRAGIA +E E+S         +R 
Sbjct: 121 RHRQQGREIDLDREFESILRRRRRSSATILQLLQGIRAGIA-SEYESS---------DRD 180

Query: 185 ILINPFNQTIVVQGGE--NQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQ 244
           +L    NQ+ VVQG    NQN+N+ S+ ++GDYFVG  LD LL+H+A+ND  R+G+ PA+
Sbjct: 181 LL----NQSAVVQGSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDSIRHGSLPAR 240

Query: 245 KEAVDALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCP 304
           KE VD LPTV++       LQCS+CLD+F+   EAKEMPCKHKFH  CI+PWLELHSSCP
Sbjct: 241 KEVVDNLPTVKI----SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCP 300

Query: 305 VCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPF 364
           VCR++LP D+  +        R + ++N  N N       E + RNS+ S RRFSFPWPF
Sbjct: 301 VCRYELPPDDETKVNPVRPRTRTL-EINVSNEN------VEDDARNSNVSERRFSFPWPF 356

Query: 365 NGLFTSQGNSSAGSASDSQRDHTS 366
           +GLF+S  +SSA ++  S     S
Sbjct: 361 SGLFSSSSSSSASASGSSHLGENS 356

BLAST of MELO3C014733.jh1 vs. TAIR 10
Match: AT4G26400.1 (RING/U-box superfamily protein )

HSP 1 Score: 319.7 bits (818), Expect = 3.1e-87
Identity = 192/384 (50.00%), Postives = 249/384 (64.84%), Query Frame = 0

Query: 5   IATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNELESDF 64
           ++ RYWCHMCSQMVNP++  EIKCPFC+SGFVEE+   I   N  ++++     + E DF
Sbjct: 1   MSARYWCHMCSQMVNPVIGAEIKCPFCQSGFVEEMSREI---NGGSSSSLREVQDPEIDF 60

Query: 65  GSERALSLWAPILLGMMGNPRGRRRFRHLEFD-DDDDDNEHEDGEGN------------H 124
           G++RALSLW PILLGMM NPR RRRFR  EF  D+D+ N  +D +GN            H
Sbjct: 61  GTDRALSLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRH 120

Query: 125 VSSE-------TTELDSVI-RRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERV 184
              +         E +S++ RRRRSSATILQLLQGIRAGIA +E E+S         +R 
Sbjct: 121 RHRQQGREIDLDREFESILRRRRRSSATILQLLQGIRAGIA-SEYESS---------DRD 180

Query: 185 ILINPFNQTIVVQGGE--NQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQ 244
           +L    NQ+ VVQG    NQN+N+ S+ ++GDYFVG  LD LL+H+A+ND  R+G+ PA+
Sbjct: 181 LL----NQSAVVQGSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDSIRHGSLPAR 240

Query: 245 KEAVDALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCP 304
           KE VD LPTV++       LQCS+CLD+F+   EAKEMPCKHKFH  CI+PWLELHSSCP
Sbjct: 241 KEVVDNLPTVKI----SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCP 300

Query: 305 VCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSSESGRRFSFPWPF 364
           VCR++LP D+  +        R + ++N  N N       E + RNS+ S RRFSFPWPF
Sbjct: 301 VCRYELPPDDETKVNPVRPRTRTL-EINVSNEN------VEDDARNSNVSERRFSFPWPF 356

Query: 365 NGLFTSQGNSSAGSASDSQRDHTS 366
           +GLF+S  +SSA ++  S     S
Sbjct: 361 SGLFSSSSSSSASASGSSHLGENS 356

BLAST of MELO3C014733.jh1 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 311.2 bits (796), Expect = 1.1e-84
Identity = 183/375 (48.80%), Postives = 241/375 (64.27%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60
           M+E + TRYWCHMCSQ VNP+++ EIKCPFC+SGFVEE+ +    D++D  +   NN   
Sbjct: 1   MEEAVVTRYWCHMCSQTVNPVMEAEIKCPFCQSGFVEEMEDDDDHDSSDPADVRANN--- 60

Query: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDD-----DNEHEDGEGNHVSSE 120
                     SLWAPIL+ +M +P  RRR + +E  +D+      +N  +DGE N +  +
Sbjct: 61  ----------SLWAPILMELMNDPVRRRRNQSVESVEDNQNEVQTENNEDDGE-NDLDWQ 120

Query: 121 TTELDSVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQTIVV 180
             E+  + RRRR SA +LQLLQGIRAG+ + ESE++  G       RVILIN  NQTI V
Sbjct: 121 LQEI--LRRRRRHSAAVLQLLQGIRAGL-SVESEST--GNGGNNPGRVILINTSNQTITV 180

Query: 181 QGGENQNQNHNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVEL 240
           Q   + + +    GSLGDYF+GPG ++LLQ +AENDPNRYGTPPA+KEAV+AL TV++E 
Sbjct: 181 Q--NSADMDSVPAGSLGDYFIGPGFEMLLQRLAENDPNRYGTPPAKKEAVEALATVKIE- 240

Query: 241 EEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD 300
                LQCSVCLD+FE+  EAK MPC HKFH+ C+LPWLELHSSCPVCR+QLP DE+K D
Sbjct: 241 ---ETLQCSVCLDDFEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPADEAKTD 300

Query: 301 -----VDGGSAARFMSDLNNGNGNGNGN--------GSGEIEERNSSESGRRFSFPWPFN 358
                 D   ++   +  ++G  N +GN           E EE N +  G  FS PWPF+
Sbjct: 301 SVTTTSDNNGSSSASATTSHGAENSDGNRRQEEEEEEEEEEEEENENNDGSGFSIPWPFS 350

BLAST of MELO3C014733.jh1 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.5e-65
Identity = 159/342 (46.49%), Postives = 207/342 (60.53%), Query Frame = 0

Query: 1   MDELIATRYWCHMCSQMVNPIVDVE-IKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNE 60
           M++   T YWCHMCS+ V P++  E IKC FC+SGFVEE+      DNND++        
Sbjct: 1   MEDASETSYWCHMCSRSVIPLIQDEIIKCNFCQSGFVEEM------DNNDDH-------- 60

Query: 61  LESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEF-----DDDDDDNEHEDGEGNHVS- 120
                  + A SLW PIL+ MM N   +      +      D+D+D+++ +DG+ N+   
Sbjct: 61  -------QAADSLWTPILMEMMNNNHDQHSTNQEDSESILEDEDEDEDDGDDGDQNNDGE 120

Query: 121 -SETTELDSVIR-RRRSSATILQLLQGIRAGIATAESENSYEGERSRERERVILINPFNQ 180
              T +L+ + R R R S  I+ LLQGIRAG+   ESEN+ +   +   E V+LIN FNQ
Sbjct: 121 IDITHQLEEIRRIRTRHSTAIVNLLQGIRAGL-LIESENNEDNPDN--SELVVLINSFNQ 180

Query: 181 TIVVQGGENQNQNHNSIGSLGDYFVGPGLDLLLQHIAEND-PNRYGTPPAQKEAVDALPT 240
            I V        +  S GSLGDYF+GPG + LLQ +AEND  NRYGTPPA KEAV+AL  
Sbjct: 181 RIRVHQDSVDTTSVPS-GSLGDYFIGPGFETLLQRLAENDLNNRYGTPPATKEAVEALAM 240

Query: 241 VRVELEEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP-- 300
           V++   EDS LQCSVCLD+FE+  EAKEMPCKHKFH+ C+LPWLELHSSCPVCR+ LP  
Sbjct: 241 VKI---EDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCRYLLPTG 300

Query: 301 -GDESKRDVDGGSAARFMSDLNNGNGNGNGNGSGEIEERNSS 330
             DE K D +         D++N +   NG+ S +    NSS
Sbjct: 301 DDDEPKTDAETSRNDDNNEDISNASMASNGS-SPDSSSNNSS 313