MELO3C014598 (gene) Melon (DHL92) v4

Overview


Name	MELO3C014598
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	tRNA-5-taurinomethyluridine 2-sulfurtransferase
Location	chr05: 1063152 .. 1067925 (-)
RNA-Seq Expression	MELO3C014598
Synteny	MELO3C014598

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATTGGATAATATGTTTTTCGCCTAACGGGTTCGTCTTCCGTCCGGAGATGCGACGGCGGTGGATTGAATGATGCTGAGACTGAGAGTGAATCCCGCCGTGTGGTGCTCTGTTCCGATTTCGCATTTCACCTCTCTTCCCCTTTCACCCTCGATTCTCAGACCTCTCCTCCGCCCCCACAACCCTCGTCTCTTCTTCACCCGATTCTCCGCTTCCTCTGCCGTGCAGCATAATACCTCCCAATCTTCTTTACCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCGATGCCTACTTATCCCCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGTCTGTTATGTTATATGGTTCTTGTGTTTTCTTGCAAATTATGGTAGCTGATATAGTGAATGCTGGTGCAATTGTGAGCAATCAAATGGGCATTTTCAGCTCGAAGGAAATATAGCCTTTTCGTGTGAACGGACTTTAATGGGTTCTGTTTTCGTAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTTGTTACTATTTTAGCTTATAAATCCATCTAAACTTGCAAAGGTTAATTTTTCCAACGAATTTTCAAATGAAATAAAGGATTTGTTGGGAAAATTAAACATTATTCAAGATACCCACAAGTAATGTCTTTCTTCTGTAGTTTGTGCTCAAGAGAGATCTTTGAGCTGAAAATGTTATGTAAATTGGTTTTCAGATTGATGTACCGTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTAAGTGCATTTACAACTACAATTTTATCCTATAACTTTGTTGGATGTGTTAACGATATATTAATACATCCAATCAATTTACTTGTAGCTGTTCCAGCTGACCATCTTTCTCATTCATTCTTTTCTAGTAGCACAAACTGGTATTCGGTTCTCATTTATGTCTACTGAATACTGTCTATTTTATGCTTTTAGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCCGATGTCCTTTGTAACACAAGAATAAAGTTTGGTATGTTCATTAGATGGACATTGGTTATATCGTCAAGCTTTCTATTAATGTTTATGATTTCTTTTGTATTAATCTAATATATGGCAAGCCCTGCTTTTTCCAGGAGCATTCATGGATGCGATAAGCAATATGGAGTTCGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCGGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGATATGGTATCTGGGAACGATTGCACAGACATTTCTTTTAATCTAACCTGTCCATTAGGGAGTATCGGTTATTCCTTGCTTAACTTTAATATTTTGTGGTAGGGAAAGGGATCATAGTGAGATTTGGTCTTTCATTAGATTTCATGTGTCTCTTTGAGCTTTGATTTCAAAGTCCTTTTTTAATTATTCAATAGGTGGTATCTTTCTCAGTTGGTCCCCCTTTCTTTAGTTAGGGTGTTTTCTGTGGGTTGTTTTTTTTGTATGCCCTTGTATTCTTGTGATTTCTCATGAAAAAATTGTAGTGGATTGTTTTTGTAACCCTTTAGGTAGGACACTATTTTGCTATTTTCATGACTTTTTTCTATATTCTACACTACACATGGCAGTCAGTAACGCCTATAGTAATTTAACTTGAAGTTTCATGTATAAAGTCAGTGGAATAGATATGACTAATATTGCATTATACGGCCACTATTTGTTGCAATCCTTCAGGAAACAACATCATTAATAAGCTTTATGATTTATATATCGCTTACCTAGTTCAAGGTTTATCATCAAACACATTTATCCCTTCCTTGTTCCTTCTTGCTCTAAAATAAAGATTTCTTATTTTACTTGATTATTATTGTTCTTAGGTCAAGGATCAAACGTATTTTCTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTCGGTTGTATTCCAAAGGTTTGTTCAAGCTAATTTTATAGTATTTATTATGATGTATTTTTTTGAGGTATCTCTTATGTTTCAGACTTCAGGGTGTATTCCGAGATGTTCAAGTTCAACTATTGTAAATTTGTACAATTTGGTACTGGCCTTCTAAGCTTTGTACAGACATTAACACCTAGTGGCTAACATTGGTTGCATTCAGTTGAGAAATTTATGTATTTAATAAAAGCTAAAAACTGTATTACAGACTTTCGCAAGCCAAATGATGTAGATAACATTTTAGAGATTTGTGGATGTTATATTGCAGTTTGAACCCATTTAGTTTTTACTATTCTCGTGTGCAAAACTTATATAAGTGCCTCAATTTATTAGTTCATATCATGCCAACTCCAATCCCAACTAGCTATATTCTTTCAACTTATGCTCTATCTTTTCTGAAGGAAAAAGGAATACTTGTGTGCTTGTTATCTTGTTAATATGTTTTTCTAGGGTTGTGACTTCAGCAATATTTTTCTTCTCTCAGCCTTATATTCCCTCAACAATCATCTGCAGGATGAGGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGGTACTTTGGATGTGGTGATTCATACATTTTTTTTTTTGTTTGTTTGTCTTTTTTCACGGGAGGCTAATGACTTTCTCAAAATTCATTGGTCAATGCTGATCTATTTCTAATATTTTGGTGTTGCTACTTTATATTTTAGAATATTCTTTACTACTTTTTAGAACAATTTATAGTAACATAGGCTGGCTTAATGGATTTAGTACAAGATTTGCCAACTCTTGTTGAAATTTTCTGATGGCTATACTTTTCCTGAGCAAGTGTTTTATTTCCTTATTCTTCATGACAGCAACAATTTTCATTCATCCTGTTTCTGAAGATGGGTCTCCTGTTTAAGGTATCTTGTGGTAGAATTCATGTAGAACCTGTTTCTTTGATGTGTAGATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTAGAGGCAGAGTCGGGAGATTTCCTTGGCAAACATCGAGGATTTTGGTTCTACACTATGGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGCCTTCTCAGTTTCTTTTCTTTAAGAATAAATTTGATGATCTCTAGCTGTTGTATGGCCATTTTACCAAAACTTCAAGAACATCTTCAACTCTATTTGGTGCAGAGATTTGAGCATTCCGATGTGCCAGTTTAGTTTTTAGTGACCGATTACATTGGGATTATTTCCAATCTGATGCTTCACAAGCTCACATTCTTTACTTACGTCTCTTGCTGAGCTAACAATGACGATTTCAGCTTTTTTGCCTTCGTCTAATGCAGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGAAACTATTTCTCATTTGACAAAAGGAGGCGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTATGCCTCCAGACCGATTTACTGGGCTTCAATGTAAGGTATAGTTTCAGCTTCCATCTCCCATATTTCTTGTTAGTTTCACTACTTCAAGTTGGCCTGGCCATAACGATTTACCATAAAAGGAAAATTTCTTAACTTTTTTACAAACCTTTCCAGAGAACCCATCAGTTTATTCCTGCAAGTTTGTGTTGGAAGTTTGACAAGCATGCTTGGCAATATTGATAATCCGGAAATGTTGACTATTACATAGAAAAGTAATTTATCTTCCATGATTATTTAATAGAACAAGCATCTCGATATCATCGATTTTTCTTTCAAACAAAGGATTTAAGTTTAGAATTCAATTTTATCTGTTAAGGGACTTTTTTTGGGGGCCTTTCTCTTTGAGAGGGGAAAATGTATTAAGATTAAGTAGATCTAGAAATGTTATGGGGAGGCTTTGTCTGCTATATCCCGCCCATTTGCTTTGAGATTGTGTAATTGGACTCGAGTTCTCATCTGTCATTTCCAGTTCATTGTTTCATAATTTTATTCTATTTCTAGGTCCGACATGGCCCGGGTTTCTATAGTTGCAATTTGACAATGGAACACAATAAGGATGGTCATGAAGATGTTGCAGTCGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCCGCCTTTTATAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGATCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCACAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGACAGTGGTATGAACGAATCTAAAGCCAAAGATGATACAAAAATACAAGACAAGTCAAGGAACGAAGAAGCCACTGTGGTTGAGCAAATGAGAAAAGTATCTCCAGATGAAGCAATGGCCACAACCCCTATGAAATGGCTTGGAAGCCTTAGGAAGAAGTTGCTGCAGATATTTTAGGAGAAGTATCTTTTGAGTGGACTATCACATAATTGCATGTTTGTAATGGAGCATATATCATGGATAAAATAGTTAAGATGTAAGAACTTTTAAAATTAGAGGTCTAAATTGAAATCACGAGGT

mRNA sequence

AAATTGGATAATATGTTTTTCGCCTAACGGGTTCGTCTTCCGTCCGGAGATGCGACGGCGGTGGATTGAATGATGCTGAGACTGAGAGTGAATCCCGCCGTGTGGTGCTCTGTTCCGATTTCGCATTTCACCTCTCTTCCCCTTTCACCCTCGATTCTCAGACCTCTCCTCCGCCCCCACAACCCTCGTCTCTTCTTCACCCGATTCTCCGCTTCCTCTGCCGTGCAGCATAATACCTCCCAATCTTCTTTACCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCGATGCCTACTTATCCCCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGATTGATGTACCGTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCCGATGTCCTTTGTAACACAAGAATAAAGTTTGGAGCATTCATGGATGCGATAAGCAATATGGAGTTCGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCGGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGATATGGTCAAGGATCAAACGTATTTTCTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTCGGTTGTATTCCAAAGGATGAGGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTAGAGGCAGAGTCGGGAGATTTCCTTGGCAAACATCGAGGATTTTGGTTCTACACTATGGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGAAACTATTTCTCATTTGACAAAAGGAGGCGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTATGCCTCCAGACCGATTTACTGGGCTTCAATGTAAGGTCCGACATGGCCCGGGTTTCTATAGTTGCAATTTGACAATGGAACACAATAAGGATGGTCATGAAGATGTTGCAGTCGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCCGCCTTTTATAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGATCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCACAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGACAGTGGTATGAACGAATCTAAAGCCAAAGATGATACAAAAATACAAGACAAGTCAAGGAACGAAGAAGCCACTGTGGTTGAGCAAATGAGAAAAGTATCTCCAGATGAAGCAATGGCCACAACCCCTATGAAATGGCTTGGAAGCCTTAGGAAGAAGTTGCTGCAGATATTTTAGGAGAAGTATCTTTTGAGTGGACTATCACATAATTGCATGTTTGTAATGGAGCATATATCATGGATAAAATAGTTAAGATGTAAGAACTTTTAAAATTAGAGGTCTAAATTGAAATCACGAGGT

Coding sequence (CDS)

ATGATGCTGAGACTGAGAGTGAATCCCGCCGTGTGGTGCTCTGTTCCGATTTCGCATTTCACCTCTCTTCCCCTTTCACCCTCGATTCTCAGACCTCTCCTCCGCCCCCACAACCCTCGTCTCTTCTTCACCCGATTCTCCGCTTCCTCTGCCGTGCAGCATAATACCTCCCAATCTTCTTTACCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCGATGCCTACTTATCCCCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGATTGATGTACCGTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCCGATGTCCTTTGTAACACAAGAATAAAGTTTGGAGCATTCATGGATGCGATAAGCAATATGGAGTTCGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCGGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGATATGGTCAAGGATCAAACGTATTTTCTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTCGGTTGTATTCCAAAGGATGAGGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTAGAGGCAGAGTCGGGAGATTTCCTTGGCAAACATCGAGGATTTTGGTTCTACACTATGGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGAAACTATTTCTCATTTGACAAAAGGAGGCGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTATGCCTCCAGACCGATTTACTGGGCTTCAATGTAAGGTCCGACATGGCCCGGGTTTCTATAGTTGCAATTTGACAATGGAACACAATAAGGATGGTCATGAAGATGTTGCAGTCGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCCGCCTTTTATAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGATCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCACAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGACAGTGGTATGAACGAATCTAAAGCCAAAGATGATACAAAAATACAAGACAAGTCAAGGAACGAAGAAGCCACTGTGGTTGAGCAAATGAGAAAAGTATCTCCAGATGAAGCAATGGCCACAACCCCTATGAAATGGCTTGGAAGCCTTAGGAAGAAGTTGCTGCAGATATTTTAG

Protein sequence

MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSLPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF

Homology

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BLAST of MELO3C014598 vs. NCBI nr
Match: XP_008449720.1 (PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo] >TYJ96142.1 tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa])

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
           LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of MELO3C014598 vs. NCBI nr
Match: KAA0041410.1 (tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa])

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 536/539 (99.44%), Postives = 539/539 (100.00%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
           LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSGMPPD+FTGLQCKVRHGPGFYSCNLTMEHN+DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           PDSGMNESKAKDDTKIQD+SRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of MELO3C014598 vs. NCBI nr
Match: XP_004142116.1 (uncharacterized protein LOC101204779 isoform X1 [Cucumis sativus] >KGN54175.1 hypothetical protein Csa_017959 [Cucumis sativus])

HSP 1 Score: 1054.3 bits (2725), Expect = 3.6e-304
Identity = 514/539 (95.36%), Postives = 525/539 (97.40%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPL+PSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
             KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  SSKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG PPD+FT LQCKVRHGP FYSCNLTMEH++DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           P+SGM ESKAKDDT+I DK RNEEATVVEQ+R+VSPDEAMAT  MKWLGSL+KKLLQIF
Sbjct: 481 PESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

[Close]

BLAST of MELO3C014598 vs. NCBI nr
Match: XP_038901654.1 (tRNA-specific 2-thiouridylase MnmA [Benincasa hispida])

HSP 1 Score: 1008.8 bits (2607), Expect = 1.7e-290
Identity = 487/538 (90.52%), Postives = 512/538 (95.17%), Query Frame = 0


Query: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61
           MLRLRV P VWCS+PISHFTSLPLSPSILRP+LR  NPRLF TRFSASSA+   T +SS 
Sbjct: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSAL-CKTLESSS 60

Query: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121
           PK LSDAD +PFLSCSMP+YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  PKGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240

Query: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300

Query: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361
           EGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360

Query: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421
           RRRVFRVGSLKWL+GMPPD+F+GLQCKVRHGP FYSCNLTMEHN+DG ED+AVVHLSEDD
Sbjct: 361 RRRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDD 420

Query: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481
           QGLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENAQMEDKSKLGKPIKIKVKP
Sbjct: 421 QGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKP 480

Query: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           +S + ES+AKDDTKI  +S N+E TV++Q+ +VS DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 481 ESVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 537

[Close]

BLAST of MELO3C014598 vs. NCBI nr
Match: XP_022944568.1 (uncharacterized protein LOC111448983 [Cucurbita moschata])

HSP 1 Score: 969.5 bits (2505), Expect = 1.2e-278
Identity = 472/538 (87.73%), Postives = 498/538 (92.57%), Query Frame = 0


Query: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61
           MLRLRVNP VWC++PISHFTSLPL PSILRP+LR H PRLF TRFSASSAV H  S+SS 
Sbjct: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAV-HKASESSS 60

Query: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121
            K LSD D  PFLSCSMP +PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  AKGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSH 240

Query: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300

Query: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361
           EGVILEAESGDFLGKHRGFWFYT+GQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360

Query: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421
           RRR+FRVGSLKWLSG+ PD+FT LQCKVRHGP FYSCNLTME N+D  EDVAVVHLSEDD
Sbjct: 361 RRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDD 420

Query: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481
           QGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKP
Sbjct: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480

Query: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           ++   E + KDDTKI    +N++ TVVEQ  +V+ DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 481 ENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of MELO3C014598 vs. ExPASy Swiss-Prot
Match: Q0SMH1 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN=mnmA PE=3 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 4.5e-100
Identity = 170/365 (46.58%), Postives = 249/365 (68.22%), Query Frame = 0


Query: 83  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSSVAL R+++    S   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYTSIKCYYLKIWLEDEL-SYIGSCPWQEDLNYVEAV 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 202
           C + +VP E+++  +EY+  VVSY I+E + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CSKFNVPYEIINFQKEYYNKVVSYTIKELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            V +GHYA +        DK   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QTKDKKFFLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           VR++A   NLPNK+RKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKNRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGTHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++G P D 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYSKQAKRKFLVHEIHWINGTPSD- 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 442
           F   + K+RHG   YSC L +       +++  + L++ D G++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLI-----AKNLIEISLNKKDYGISPGQFAIFYKNTECLGG 352

Query: 443 GVILE 447
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of MELO3C014598 vs. ExPASy Swiss-Prot
Match: Q660I8 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=290434 GN=mnmA PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 5.9e-100
Identity = 168/365 (46.03%), Postives = 250/365 (68.49%), Query Frame = 0


Query: 83  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSS+AL ++++    +   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSIALYKVINKGYTNIKCYYLKIWLEDEL-SYIGNCPWQEDLNYVEAV 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 202
           C++ ++P E+++  +EY+  VV Y IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNIPYEIINFQKEYYNKVVIYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            V +GHYA +        +K   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYARI-----QIKEKKFFLKQAKDKIKDQSYFLSHLSQEQMSKLYFPLGTLFKSE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKSINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIVEKETGKIIGIHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++GMP D 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINGMPSD- 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 442
           F   + K+RHG   YSC L +  N     ++  + L++ D G++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLRLITN-----NLIEIFLNKKDHGISPGQFAIFYKNTECLGG 352

Query: 443 GVILE 447
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of MELO3C014598 vs. ExPASy Swiss-Prot
Match: O51625 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=mnmA PE=3 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.0e-99
Identity = 168/365 (46.03%), Postives = 250/365 (68.49%), Query Frame = 0


Query: 83  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 202
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++  P + 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINDTPTN- 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 442
           F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 443 GVILE 447
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of MELO3C014598 vs. ExPASy Swiss-Prot
Match: B7J2N7 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=445985 GN=mnmA PE=3 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.0e-99
Identity = 168/365 (46.03%), Postives = 250/365 (68.49%), Query Frame = 0


Query: 83  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 202
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQISKLYFPLGTLLKSE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++  P + 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINDTPTN- 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 442
           F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 443 GVILE 447
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of MELO3C014598 vs. ExPASy Swiss-Prot
Match: B2S125 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314723 GN=mnmA PE=3 SV=2)

HSP 1 Score: 352.4 bits (903), Expect = 8.8e-96
Identity = 166/366 (45.36%), Postives = 244/366 (66.67%), Query Frame = 0


Query: 83  LKVAVLLSGGVDSSVALRLLHAAGH-SCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSSVAL  +   G+ +   +YLKIW +++  ++  ECPW++D+ Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDEL-SYIGECPWEEDINYVEAV 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 202
           C + +VP E++ L +EY++ VV+Y IEE + G TP+PD+ CN RIKFGAF D I N  +D
Sbjct: 61  CTKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKI-NEHYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            + +GHYA +     +  +   +L+ +KD +KDQ+YFLSHLS+ Q+ +L FPLG + K E
Sbjct: 121 LIVTGHYAKI-----ENKNNHYILKQAKDKIKDQSYFLSHLSREQISKLHFPLGDLLKTE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           +R++A K +LPNK+RKDSQGICFLGKIK+ EF+  H+GE +G I+E E+G  LG H G+W
Sbjct: 181 IRQIAHKIDLPNKNRKDSQGICFLGKIKYDEFIKYHLGELKGNIIEQETGKILGTHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V EKDI+NN++++S +     + +  F V    W++   P  
Sbjct: 241 FFTIGQRKGIKLSHGPWFVTEKDIQNNIIYISNSVNYLAQGKHQFLVHKTNWIN--KPVN 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 442
              L  K+RHG     C + M  N     D+  V L E D G++ GQF  FY+   C+G 
Sbjct: 301 NIDLSAKIRHGEKKIKCKIEMLKN-----DIIKVDLEEKDYGISPGQFCIFYQEDECLGG 352

Query: 443 GVILES 448
             IL++
Sbjct: 361 AQILQT 352

[Close]

BLAST of MELO3C014598 vs. ExPASy TrEMBL
Match: A0A5D3B8G8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001100 PE=3 SV=1)

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
           LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of MELO3C014598 vs. ExPASy TrEMBL
Match: A0A1S3BNL8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo OX=3656 GN=LOC103491516 PE=3 SV=1)

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
           LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of MELO3C014598 vs. ExPASy TrEMBL
Match: A0A5A7TDN6 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00570 PE=3 SV=1)

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 536/539 (99.44%), Postives = 539/539 (100.00%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
           LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSGMPPD+FTGLQCKVRHGPGFYSCNLTMEHN+DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           PDSGMNESKAKDDTKIQD+SRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of MELO3C014598 vs. ExPASy TrEMBL
Match: A0A0A0KX16 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1)

HSP 1 Score: 1054.3 bits (2725), Expect = 1.7e-304
Identity = 514/539 (95.36%), Postives = 525/539 (97.40%), Query Frame = 0


Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPL+PSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
             KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  SSKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG PPD+FT LQCKVRHGP FYSCNLTMEH++DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           P+SGM ESKAKDDT+I DK RNEEATVVEQ+R+VSPDEAMAT  MKWLGSL+KKLLQIF
Sbjct: 481 PESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

[Close]

BLAST of MELO3C014598 vs. ExPASy TrEMBL
Match: A0A6J1FY63 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN=LOC111448983 PE=3 SV=1)

HSP 1 Score: 969.5 bits (2505), Expect = 5.6e-279
Identity = 472/538 (87.73%), Postives = 498/538 (92.57%), Query Frame = 0


Query: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61
           MLRLRVNP VWC++PISHFTSLPL PSILRP+LR H PRLF TRFSASSAV H  S+SS 
Sbjct: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAV-HKASESSS 60

Query: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121
            K LSD D  PFLSCSMP +PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  AKGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSH 240

Query: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300

Query: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361
           EGVILEAESGDFLGKHRGFWFYT+GQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360

Query: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421
           RRR+FRVGSLKWLSG+ PD+FT LQCKVRHGP FYSCNLTME N+D  EDVAVVHLSEDD
Sbjct: 361 RRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDD 420

Query: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481
           QGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKP
Sbjct: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480

Query: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           ++   E + KDDTKI    +N++ TVVEQ  +V+ DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 481 ENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of MELO3C014598 vs. TAIR 10
Match: AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases )

HSP 1 Score: 716.1 bits (1847), Expect = 2.2e-206
Identity = 352/477 (73.79%), Postives = 394/477 (82.60%), Query Frame = 0


Query: 28  SILRPLLRPHNP-------------RLFFTRFSASSAVQHNTSQSSLP-KPLSDAD---- 87
           ++ +P L P  P             R+F + FS+SS+ Q  + QSS P  PL  ++    
Sbjct: 4   AVAKPFLSPSPPLFRLPLSSISISFRVFPSFFSSSSSSQLPSPQSSNPITPLISSNRNYS 63

Query: 88  ---FL--PFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFEN 147
              F+   +LSCSMP  PL+VAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE FEN
Sbjct: 64  RHAFIVDQYLSCSMPEKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFEN 123

Query: 148 FWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNT 207
           FW++CPW+DDLKYAK VC+Q+DVPLEVVHLT+EYW  VVSYIIEEYRCGRTPNPDVLCNT
Sbjct: 124 FWNQCPWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNT 183

Query: 208 RIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQ 267
           RIKFGAFMDAIS+ME+DYV SGHYA V+HP ADQ D  SVLELS+DMVKDQTYFLSHLSQ
Sbjct: 184 RIKFGAFMDAISDMEYDYVGSGHYAKVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQ 243

Query: 268 NQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGV 327
            QLKRLLFPLGC+ KDEVRKLA +F+LPNKDRKDSQGICFLGKIKFS+FV RHIGE EG+
Sbjct: 244 TQLKRLLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCRHIGEMEGI 303

Query: 328 ILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRR 387
           ILEAESGDFLG HRGFWFYT+GQRQGLRLPGGPWYVVEKD KNNVVFVSRNY+S DKRRR
Sbjct: 304 ILEAESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRR 363

Query: 388 VFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGL 447
           +FRVGSL+W SG P  +   L+CKVRHGPGFYSC+  ME    G  DVAVVHL EDDQGL
Sbjct: 364 IFRVGSLRWNSGKPSGKVRELRCKVRHGPGFYSCSFEME----GDGDVAVVHLDEDDQGL 423

Query: 448 AAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 482
           AAGQFAAFY+G +CIGSGVILESWDDQ FPVC KA++ A +EDK+KLGKP+KI   P
Sbjct: 424 AAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAALEDKTKLGKPVKIMTMP 476

The following BLAST results are available for this feature:

BLAST of MELO3C014598 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008449720.1	0.0e+00	100.00	PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo] >TYJ96142.1 tRNA-sp...	[more]
KAA0041410.1	0.0e+00	99.44	tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa]	[more]
XP_004142116.1	3.6e-304	95.36	uncharacterized protein LOC101204779 isoform X1 [Cucumis sativus] >KGN54175.1 hy...	[more]
XP_038901654.1	1.7e-290	90.52	tRNA-specific 2-thiouridylase MnmA [Benincasa hispida]	[more]
XP_022944568.1	1.2e-278	87.73	uncharacterized protein LOC111448983 [Cucurbita moschata]	[more]

BLAST of MELO3C014598 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0SMH1	4.5e-100	46.58	tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN...	[more]
Q660I8	5.9e-100	46.03	tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496...	[more]
O51625	1.0e-99	46.03	tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / ...	[more]
B7J2N7	1.0e-99	46.03	tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=44598...	[more]
B2S125	8.8e-96	45.36	tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314...	[more]

BLAST of MELO3C014598 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3B8G8	0.0e+00	100.00	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3BNL8	0.0e+00	100.00	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5A7TDN6	0.0e+00	99.44	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1...	[more]
A0A0A0KX16	1.7e-304	95.36	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Cs...	[more]
A0A6J1FY63	5.6e-279	87.73	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of MELO3C014598 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G51310.1	2.2e-206	73.79	transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF03054	tRNA_Me_trans	coord: 84..444 e-value: 1.9E-125 score: 418.3
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 363..452 e-value: 2.8E-13 score: 51.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 479..509
None	No IPR available	PANTHER	PTHR43052	FAMILY NOT NAMED	coord: 72..477
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 84..329
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 75..296 e-value: 2.1E-85 score: 287.6
IPR023382	Adenine nucleotide alpha hydrolase-like domains	GENE3D	2.30.30.280		coord: 297..356 e-value: 3.5E-18 score: 66.7
IPR004506	tRNA-specific 2-thiouridylase	TIGRFAM	TIGR00420	TIGR00420	coord: 84..444 e-value: 1.6E-108 score: 360.6
IPR004506	tRNA-specific 2-thiouridylase	HAMAP	MF_00144	tRNA_thiouridyl_MnmA	coord: 83..445 score: 33.149933
IPR004506	tRNA-specific 2-thiouridylase	CDD	cd01998	tRNA_Me_trans	coord: 84..444 e-value: 6.96515E-159 score: 454.673

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C014598.1	MELO3C014598.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008033	tRNA processing
molecular_function	GO:0016783	sulfurtransferase activity
molecular_function	GO:0016740	transferase activity