MELO3C014075 (gene) Melon (DHL92) v4

BLAST of MELO3C014075 vs. NCBI nr
Match: XP_016900593.1 (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo])

HSP 1 Score: 1424.1 bits (3685), Expect = 0.0e+00
Identity = 706/706 (100.00%), Postives = 706/706 (100.00%), Query Frame = 0

Query: 1   MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 60
           MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND
Sbjct: 148 MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 207

Query: 61  IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR 120
           IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR
Sbjct: 208 IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR 267

Query: 121 KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 180
           KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG
Sbjct: 268 KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 327

Query: 181 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 240
           GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI
Sbjct: 328 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 387

Query: 241 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 300
           AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV
Sbjct: 388 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 447

Query: 301 SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD 360
           SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD
Sbjct: 448 SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD 507

Query: 361 KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT 420
           KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT
Sbjct: 508 KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT 567

Query: 421 SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR 480
           SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR
Sbjct: 568 SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR 627

Query: 481 IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK 540
           IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK
Sbjct: 628 IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK 687

Query: 541 ATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKV 600
           ATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKV
Sbjct: 688 ATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKV 747

Query: 601 TDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGE 660
           TDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGE
Sbjct: 748 TDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGE 807

Query: 661 EKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 707
           EKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN
Sbjct: 808 EKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 853

BLAST of MELO3C014075 vs. NCBI nr
Match: XP_031738719.1 (uncharacterized protein LOC101212014 [Cucumis sativus])

HSP 1 Score: 1355.5 bits (3507), Expect = 0.0e+00
Identity = 664/707 (93.92%), Postives = 688/707 (97.31%), Query Frame = 0

Query: 1    MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 60
            MAEEAIFYSYTRSFNGFAA LDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND
Sbjct: 838  MAEEAIFYSYTRSFNGFAAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 897

Query: 61   IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR 120
            IGIPSNSIWN AKFGEDVI+ANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDS FHCNR
Sbjct: 898  IGIPSNSIWNTAKFGEDVIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNR 957

Query: 121  KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 180
            KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG
Sbjct: 958  KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 1017

Query: 181  GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 240
            GAPKARAVAYK CWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI
Sbjct: 1018 GAPKARAVAYKACWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 1077

Query: 241  AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 300
            AAFLAVQRGILVVFSGGNSGPFPMT+ N+SPWVFTVAA+TIDR+F SYVGLGNKKH+KG+
Sbjct: 1078 AAFLAVQRGILVVFSGGNSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGL 1137

Query: 301  SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD 360
            SLSSV SLPKKF+PLINSVDAKF NVTEFHAQFCGKGTLDPMKVKGKIVICQVGET+GVD
Sbjct: 1138 SLSSVPSLPKKFFPLINSVDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVD 1197

Query: 361  KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT 420
            KG+QAS AGA GVI+AND+EKGDEI+PELHFIPASDITNTDAQIVQ YLKST+TP+AHLT
Sbjct: 1198 KGFQASRAGAVGVIIANDLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLT 1257

Query: 421  SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR 480
            SVKTLLSVKPAP IATFS+RGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFS  DRRR
Sbjct: 1258 SVKTLLSVKPAPTIATFSARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRR 1317

Query: 481  IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK 540
            IPFNVISGTSMSCPHVAGIAGL+KSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK
Sbjct: 1318 IPFNVISGTSMSCPHVAGIAGLIKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK 1377

Query: 541  ATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKV 600
            ATPYAYGAGQV PNDAADPGLVYDIT+NDYLNFLCARGY+AM+IKKFYAKPF+CVRSFKV
Sbjct: 1378 ATPYAYGAGQVNPNDAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKV 1437

Query: 601  TDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGE 660
            TDLNYPSISVGELKIGAPLT+NRRVKNVGSPGTYVARVKASPGVAV+IEPSTLVF  VGE
Sbjct: 1438 TDLNYPSISVGELKIGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGE 1497

Query: 661  EKGFKVVLQNTGKVKSG-DVFGTLIWSDGKHFVRSPIAVHLGPGIGN 707
            EKGFKVVLQNTGKVK+G DVFGTLIWSDGKHFVRS IAVHLGPGIGN
Sbjct: 1498 EKGFKVVLQNTGKVKNGSDVFGTLIWSDGKHFVRSSIAVHLGPGIGN 1544

BLAST of MELO3C014075 vs. NCBI nr
Match: TYK19384.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])

HSP 1 Score: 1345.5 bits (3481), Expect = 0.0e+00
Identity = 677/714 (94.82%), Postives = 682/714 (95.52%), Query Frame = 0

Query: 1   MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 60
           MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND
Sbjct: 71  MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 130

Query: 61  IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR 120
           IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNF CNR
Sbjct: 131 IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNR 190

Query: 121 KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 180
           KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG
Sbjct: 191 KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 250

Query: 181 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 240
           GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI
Sbjct: 251 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 310

Query: 241 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 300
           AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKH+K  
Sbjct: 311 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKVH 370

Query: 301 SLSSVASL-PKKFYP-------LINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQ 360
             + +  L   +F P       L N       +  E   QFCGKGTLDPMKVKGKIVICQ
Sbjct: 371 VYTHLLGLGASRFAPNFICAPELYNQY--SIGSTLETSNQFCGKGTLDPMKVKGKIVICQ 430

Query: 361 VGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKST 420
           VGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKST
Sbjct: 431 VGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKST 490

Query: 421 KTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPT 480
           KTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPT
Sbjct: 491 KTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPT 550

Query: 481 FSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQT 540
           FSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQT
Sbjct: 551 FSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQT 610

Query: 541 ILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPF 600
           ILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPF
Sbjct: 611 ILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPF 670

Query: 601 NCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPST 660
           NCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPST
Sbjct: 671 NCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPST 730

Query: 661 LVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 707
           LVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN
Sbjct: 731 LVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 782

BLAST of MELO3C014075 vs. NCBI nr
Match: KAE8650095.1 (hypothetical protein Csa_011146 [Cucumis sativus])

HSP 1 Score: 1320.8 bits (3417), Expect = 0.0e+00
Identity = 650/698 (93.12%), Postives = 674/698 (96.56%), Query Frame = 0

Query: 1   MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 60
           MAEEAIFYSYTRSFNGFAA LDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND
Sbjct: 45  MAEEAIFYSYTRSFNGFAAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 104

Query: 61  IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR 120
           IGIPSNSIWN AKFGEDVI+ANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDS FHCNR
Sbjct: 105 IGIPSNSIWNTAKFGEDVIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNR 164

Query: 121 KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 180
           KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG
Sbjct: 165 KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 224

Query: 181 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 240
           GAPKARAVAYK CWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI
Sbjct: 225 GAPKARAVAYKACWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 284

Query: 241 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 300
           AAFLAVQRGILVVFSGGNSGPFPMT+ N+SPWVFTVAA+TIDR+F SYVGLGNKKH+KG+
Sbjct: 285 AAFLAVQRGILVVFSGGNSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGL 344

Query: 301 SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD 360
           SLSSV SLPKKF+PLINSVDAKF NVTEFHAQFCGKGTLDPMKVKGKIVICQVGET+GVD
Sbjct: 345 SLSSVPSLPKKFFPLINSVDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVD 404

Query: 361 KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT 420
           KG+QAS AGA GVI+AND+EKGDEI+PELHFIPASDITNTDAQIVQ YLKST+TP+AHLT
Sbjct: 405 KGFQASRAGAVGVIIANDLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLT 464

Query: 421 SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR 480
           SVKTLLSVKPAP IATFS+RGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFS  DRRR
Sbjct: 465 SVKTLLSVKPAPTIATFSARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRR 524

Query: 481 IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTT------AKTRGNNNQTIL 540
           IPFNVISGTSMSCPHVAGIAGL+KSIHPNWSPAAIKSAIMTT      AKTRGNNNQTIL
Sbjct: 525 IPFNVISGTSMSCPHVAGIAGLIKSIHPNWSPAAIKSAIMTTVTYSFAAKTRGNNNQTIL 584

Query: 541 DSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNC 600
           DSTKLKATPYAYGAGQV PNDAADPGLVYDIT+NDYLNFLCARGY+AM+IKKFYAKPF+C
Sbjct: 585 DSTKLKATPYAYGAGQVNPNDAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSC 644

Query: 601 VRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLV 660
           VRSFKVTDLNYPSISVGELKIGAPLT+NRRVKNVGSPGTYVARVKASPGVAV+IEPSTLV
Sbjct: 645 VRSFKVTDLNYPSISVGELKIGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLV 704

Query: 661 FRSVGEEKGFKVVLQNTGKVKSG-DVFGTLIWSDGKHF 692
           F  VGEEKGFKVVLQNTGKVK+G DVFGTLIWSDGKHF
Sbjct: 705 FSRVGEEKGFKVVLQNTGKVKNGSDVFGTLIWSDGKHF 742

BLAST of MELO3C014075 vs. NCBI nr
Match: XP_038904203.1 (uncharacterized protein LOC120090550 [Benincasa hispida])

HSP 1 Score: 1243.8 bits (3217), Expect = 0.0e+00
Identity = 607/703 (86.34%), Postives = 650/703 (92.46%), Query Frame = 0

Query: 1   MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 60
           M EEAIFYSYT SFNGFAA LD+KEA NLARNPKVISVFENKARKLHTTRSWNFLGVEND
Sbjct: 71  MTEEAIFYSYTTSFNGFAAKLDEKEAANLARNPKVISVFENKARKLHTTRSWNFLGVEND 130

Query: 61  IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR 120
           +GIPSNSIWNAAKFG+D+IIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR
Sbjct: 131 VGIPSNSIWNAAKFGQDIIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR 190

Query: 121 KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 180
           KLIGGRYF+KGY AAGG LNAT LTVRDHDGHGTHTLSTAAGNFVTGANVFGHG+GTAKG
Sbjct: 191 KLIGGRYFYKGYVAAGGTLNATSLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGSGTAKG 250

Query: 181 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 240
           GAPKAR  AYKVCWPP   SQC DADILAAFEAA+ADGVDV+STSLGGAADEYFNDPLAI
Sbjct: 251 GAPKARVAAYKVCWPPFLGSQCLDADILAAFEAAVADGVDVISTSLGGAADEYFNDPLAI 310

Query: 241 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 300
           AAF AVQ+G++VVFS GNSGPFPMTVTN++PW+ TVAA T+DRDF S V LGNK   +GV
Sbjct: 311 AAFHAVQQGVVVVFSAGNSGPFPMTVTNIAPWINTVAAGTVDRDFASNVALGNK---EGV 370

Query: 301 SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD 360
           SLSS+A LPKKFYPLI+SV+AK SNVTEFHA+FCG+GTLDPMKVKGKIVICQVGE EGV+
Sbjct: 371 SLSSIAPLPKKFYPLIDSVNAKLSNVTEFHARFCGEGTLDPMKVKGKIVICQVGEIEGVE 430

Query: 361 KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT 420
           K YQA+ AGA GVIVANDIEKGDEIYPELHFIPASDITN DAQ++QKYL ST TP+AHLT
Sbjct: 431 KSYQAARAGAVGVIVANDIEKGDEIYPELHFIPASDITNNDAQLLQKYLNSTTTPMAHLT 490

Query: 421 SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR 480
            VKTLL++KPAPIIATFSSRGPNPID  ILKPD+TAPGVNILASY TG APTFS+ D+RR
Sbjct: 491 KVKTLLNIKPAPIIATFSSRGPNPIDPFILKPDITAPGVNILASYSTGNAPTFSSNDQRR 550

Query: 481 IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK 540
           IPFNVISGTSMSCPH+AGIAGL+KSIHP+WSPAAIKSAIMTTAKTRGNN QTILDSTKLK
Sbjct: 551 IPFNVISGTSMSCPHIAGIAGLLKSIHPDWSPAAIKSAIMTTAKTRGNNLQTILDSTKLK 610

Query: 541 ATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKV 600
           AT YAYGAG V+PNDA DPGLVYD TI DYLNFLCARGY+AM++KKFYAKPFNCV+SFK 
Sbjct: 611 ATSYAYGAGMVHPNDARDPGLVYDTTIEDYLNFLCARGYNAMEMKKFYAKPFNCVKSFKA 670

Query: 601 TDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGE 660
           TDLNYPSISVG L+IGAP+TINRRVK+VGSPGTYVARVK SPGVAV +EP TL F SVGE
Sbjct: 671 TDLNYPSISVGVLRIGAPVTINRRVKSVGSPGTYVARVKVSPGVAVLVEPRTLQFSSVGE 730

Query: 661 EKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPG 704
           EK FKVVLQNTGKVK GDVFGTLIWSDGKHFVRSPIAVHLGPG
Sbjct: 731 EKAFKVVLQNTGKVKRGDVFGTLIWSDGKHFVRSPIAVHLGPG 770

BLAST of MELO3C014075 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 1.6e-225
Identity = 385/703 (54.77%), Postives = 512/703 (72.83%), Query Frame = 0

Query: 2   AEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDI 61
           A +AIFYSYT+  NGFAA LD   A  ++++P+V+SVF NKA KLHTTRSW+FLG+E++ 
Sbjct: 71  ATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNS 130

Query: 62  GIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQT--DSNFHCN 121
            +PS+SIW  A+FGED IIAN+DTGVWPESKSF DEG GP+PS+W+GICQ   D+ FHCN
Sbjct: 131 YVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCN 190

Query: 122 RKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAK 181
           RKLIG RYF+KGY AA G LN++  + RD DGHG+HTLSTAAG+FV G ++FG GNGTAK
Sbjct: 191 RKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAK 250

Query: 182 GGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLA 241
           GG+P+AR  AYKVCWPP+  ++C+DAD+LAAF+AAI DG DV+S SLGG    +FND +A
Sbjct: 251 GGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVA 310

Query: 242 IAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKG 301
           I +F A ++ I+VV S GNSGP   TV+NV+PW  TV A+T+DR+F S + LGN KH KG
Sbjct: 311 IGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKG 370

Query: 302 VSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGV 361
            SLSS A    KFYP++ SV+AK  N +   AQ C  G+LDP+K KGKI++C  G+   V
Sbjct: 371 QSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRV 430

Query: 362 DKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHL 421
           +KG   +L G  G+++ N    G+++  + H +PA+ +T+ D+  V +Y+  TK PIAH+
Sbjct: 431 EKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHI 490

Query: 422 TSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRR 481
           T  +T L +KPAP++A+FSS+GP+ +   ILKPD+TAPGV+++A+Y   ++PT    D R
Sbjct: 491 TPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPR 550

Query: 482 RIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKL 541
           R+ FN ISGTSMSCPH++GIAGL+K+ +P+WSPAAI+SAIMTTA    +    I ++T +
Sbjct: 551 RLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNM 610

Query: 542 KATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVR-SF 601
           KATP+++GAG V PN A +PGLVYD+ I DYLNFLC+ GY+A +I  F    F C     
Sbjct: 611 KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKI 670

Query: 602 KVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSV 661
            + +LNYPSI+V  L   + +T++R VKNVG P  Y  +V    GV V ++P++L F  V
Sbjct: 671 SLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKV 730

Query: 662 GEEKGFKVVL-QNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHL 701
           GE+K FKV+L ++ G V  G VFG L+WSD KH VRSPI V L
Sbjct: 731 GEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of MELO3C014075 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 2.6e-220
Identity = 388/702 (55.27%), Postives = 494/702 (70.37%), Query Frame = 0

Query: 2   AEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDI 61
           A+EAIFYSY R  NGFAAILD+ EA  +A++P V+SVF NK RKLHTT SWNF+ +  + 
Sbjct: 81  AKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNG 140

Query: 62  GIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNRK 121
            +  +S+WN A +GED IIAN+DTGVWPESKSFSDEGYG VP++W+G C  D    CNRK
Sbjct: 141 VVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHKD--VPCNRK 200

Query: 122 LIGGRYFHKGYEA-AGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 181
           LIG RYF+KGY A  G   NA+  T RDHDGHG+HTLSTAAGNFV GANVFG GNGTA G
Sbjct: 201 LIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASG 260

Query: 182 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 241
           G+PKAR  AYKVCWPP+  ++CFDADILAA EAAI DGVDVLS S+GG A +Y +D +AI
Sbjct: 261 GSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAI 320

Query: 242 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 301
            +F AV+ G+ VV S GNSGP   TV+NV+PWV TV A+++DR+F ++V L N +  KG 
Sbjct: 321 GSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGT 380

Query: 302 SLSSVASLP-KKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGV 361
           SLS    LP +K Y LI++ DA  +N     A  C KG+LDP KVKGKI++C  G+   V
Sbjct: 381 SLSK--PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARV 440

Query: 362 DKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHL 421
           DKG QA+ AGAAG+++ ND   G+EI  + H +PAS I   D + +  YL STK P  ++
Sbjct: 441 DKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYI 500

Query: 422 TSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRR 481
            +    L+ KPAP +A+FSSRGPN I   ILKPD+TAPGVNI+A++     PT   +D R
Sbjct: 501 KAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNR 560

Query: 482 RIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKL 541
           R PFN  SGTSMSCPH++G+ GL+K++HP+WSPAAI+SAIMTT++TR N  + ++D +  
Sbjct: 561 RTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFK 620

Query: 542 KATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKP-FNCVRSF 601
           KA P++YG+G V PN AA PGLVYD+T  DYL+FLCA GY+   ++ F   P + C +  
Sbjct: 621 KANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGA 680

Query: 602 KVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSV 661
            + D NYPSI+V  L     +T+ R++KNVG P TY AR +   GV V++EP  L F   
Sbjct: 681 NLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKT 740

Query: 662 GEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHL 701
           GE K F++ L+      SG VFG L W+D  H+VRSPI V L
Sbjct: 741 GEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of MELO3C014075 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 660.6 bits (1703), Expect = 2.0e-188
Identity = 363/722 (50.28%), Postives = 472/722 (65.37%), Query Frame = 0

Query: 2   AEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDI 61
           A+EAI YSY R  NGFAA+L+++EA ++A+NP V+SVF +K  KLHTTRSW FLG+    
Sbjct: 71  AKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRR- 130

Query: 62  GIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWR-GICQTDS-----N 121
               NS W   +FGE+ II NIDTGVWPES+SFSD+GYG VPSKWR G+CQ +       
Sbjct: 131 --GQNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMK 190

Query: 122 FHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGN 181
             CNRKLIG RY++K +EA  G+L+  L T RD  GHGTHTLSTA GNFV GA VF  GN
Sbjct: 191 NTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGN 250

Query: 182 GTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGA----AD 241
           GTAKGG+P+AR  AYKVCW     + C+ AD+LAA + AI DGVDV++ S G +    A+
Sbjct: 251 GTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAE 310

Query: 242 EYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGL 301
             F D ++I AF A+ + IL+V S GN GP P TV NV+PWVFT+AA+T+DRDF S + +
Sbjct: 311 GIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTI 370

Query: 302 GNKKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVIC 361
            N+  ++G SL  V   P + + LI S DAK +N T   AQ C +GTLD  KV GKIV+C
Sbjct: 371 NNQL-IEGASL-FVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLC 430

Query: 362 -QVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLK 421
            + G+ + V +G +A  AGA G+I+ N ++ G  +  E H     +     A+     +K
Sbjct: 431 TREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVK 490

Query: 422 ST---------KT-PIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVN 481
           +T         KT     ++  +TL   KPAP++A+FSSRGPN I  +ILKPDVTAPGVN
Sbjct: 491 TTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVN 550

Query: 482 ILASYPTGIAPTFSAADRRR-IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAI 541
           ILA+Y    + +    D RR   FNV+ GTSMSCPH +GIAGL+K+ HP+WSPAAIKSAI
Sbjct: 551 ILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAI 610

Query: 542 MTTAKTRGNNNQTILDS-TKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARG 601
           MTTA T  N N+ I D+  K  A  +AYG+G V P+ A +PGLVYD+++ DYLNFLCA G
Sbjct: 611 MTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASG 670

Query: 602 YDAMKIKKF-YAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVAR 661
           YD   I    + + F C  S  V DLNYPSI++  L++  P+TI R V NVG P TY   
Sbjct: 671 YDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRL-KPVTIARTVTNVGPPSTYTVS 730

Query: 662 VKASPGVAVTIEPSTLVFRSVGEEKGFKVVLQ-NTGKVKSGDVFGTLIWSDGKHFVRSPI 699
            ++  G ++ + P +L F  +GE K FKV++Q ++   +    FG L W+DGKH VRSPI
Sbjct: 731 TRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSPI 786

BLAST of MELO3C014075 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 7.6e-172
Identity = 321/703 (45.66%), Postives = 450/703 (64.01%), Query Frame = 0

Query: 6   IFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPS 65
           + Y+Y  + +GF+  L  +EA++L   P VISV      +LHTTR+  FLG++      +
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----T 124

Query: 66  NSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFH---CNRKL 125
             ++  A    DV++  +DTGVWPESKS+SDEG+GP+PS W+G C+  +NF    CNRKL
Sbjct: 125 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 184

Query: 126 IGGRYFHKGYEAAGGKLNAT--LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 185
           IG R+F +GYE+  G ++ +    + RD DGHGTHT STAAG+ V GA++ G+ +GTA+G
Sbjct: 185 IGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARG 244

Query: 186 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 245
            AP+AR   YKVCW       CF +DILAA + AIAD V+VLS SLGG   +Y+ D +AI
Sbjct: 245 MAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAI 304

Query: 246 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 305
            AF A++RGILV  S GN+GP   +++NV+PW+ TV A T+DRDF +   LGN K+  GV
Sbjct: 305 GAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGV 364

Query: 306 SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD 365
           SL    +LP K  P I + +A  SN T  +   C  GTL P KVKGKIV+C  G    V 
Sbjct: 365 SLFKGEALPDKLLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 424

Query: 366 KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT 425
           KG     AG  G+I+AN    G+E+  + H +PA+ +      I++ Y+ +   P A ++
Sbjct: 425 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 484

Query: 426 SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR 485
            + T++ VKP+P++A FSSRGPN I   ILKPD+ APGVNILA++     PT  A+D RR
Sbjct: 485 ILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRR 544

Query: 486 IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILD-STKL 545
           + FN+ISGTSMSCPHV+G+A L+KS+HP WSPAAI+SA+MTTA     + + +LD +T  
Sbjct: 545 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 604

Query: 546 KATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNC--VRS 605
            +TP+ +GAG V P  A +PGL+YD+T  DYL FLCA  Y + +I+    + + C   +S
Sbjct: 605 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS 664

Query: 606 FKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKA-SPGVAVTIEPSTLVFR 665
           + V DLNYPS +V    +GA     R V +VG  GTY  +V + + GV +++EP+ L F+
Sbjct: 665 YSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFK 724

Query: 666 SVGEEKGFKVVLQ-NTGKVKSGDVFGTLIWSDGKHFVRSPIAV 699
              E+K + V    ++ K    + FG++ WSDGKH V SP+A+
Sbjct: 725 EANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of MELO3C014075 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 2.1e-166
Identity = 324/717 (45.19%), Postives = 453/717 (63.18%), Query Frame = 0

Query: 3   EEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIG 62
           E  I + Y   F+GF+A++   EA+NL  +P V++VFE++ R+LHTTRS  FLG++N  G
Sbjct: 55  ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKG 114

Query: 63  IPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNF---HCN 122
                +W+ + +G DVII   DTG+WPE +SFSD   GP+P +WRG+C++ + F   +CN
Sbjct: 115 -----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCN 174

Query: 123 RKLIGGRYFHKGYEAAG-GKLNATL--LTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNG 182
           RK+IG R+F KG +AA  G +N T+  L+ RD DGHGTHT STAAG     A++ G+ +G
Sbjct: 175 RKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASG 234

Query: 183 TAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGA---ADEY 242
            AKG APKAR  AYKVCW    DS C D+DILAAF+AA+ DGVDV+S S+GG       Y
Sbjct: 235 VAKGVAPKARIAAYKVCWK---DSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPY 294

Query: 243 FNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGN 302
           + DP+AI ++ A  +GI V  S GN GP  M+VTN++PWV TV A+TIDR+F +   LG+
Sbjct: 295 YLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGD 354

Query: 303 KKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQV 362
              ++GVSL +   L  + +P++    +  S+     A  C + TLDP +V+GKIVIC  
Sbjct: 355 GHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSS-----ASLCMENTLDPKQVRGKIVICDR 414

Query: 363 GETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTK 422
           G +  V KG     AG  G+I+AN    G+ +  + H IPA  + + +   ++ Y  S  
Sbjct: 415 GSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHP 474

Query: 423 TPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTF 482
            PIA +    T++ +KPAP+IA+FS RGPN +   ILKPD+ APGVNILA++   + PT 
Sbjct: 475 NPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTG 534

Query: 483 SAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTI 542
             +D R+  FN++SGTSM+CPHV+G A L+KS HP+WSPA I+SA+MTT     N+N+++
Sbjct: 535 LPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSL 594

Query: 543 LD-STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPF 602
           +D ST   ATPY YG+G +    A +PGLVYDIT +DY+ FLC+ GY    I+     P 
Sbjct: 595 IDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPV 654

Query: 603 NCVRSFKVT--DLNYPSISV---GELKIGAPLTINRRVKNVG-SPGTYVARVKASPGVAV 662
            C  + K +  +LNYPSI+       +     T+ R   NVG +   Y AR+++  GV V
Sbjct: 655 RCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTV 714

Query: 663 TIEPSTLVFRSVGEEKGFKV-VLQNTGKV---KSGDVFGTLIWSD-GKHFVRSPIAV 699
           T++P  LVF S  + + + V V  NT  V   ++G VFG++ W D GKH VRSPI V
Sbjct: 715 TVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758

BLAST of MELO3C014075 vs. ExPASy TrEMBL
Match: A0A1S4DX90 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491001 PE=3 SV=1)

HSP 1 Score: 1424.1 bits (3685), Expect = 0.0e+00
Identity = 706/706 (100.00%), Postives = 706/706 (100.00%), Query Frame = 0

Query: 1   MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 60
           MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND
Sbjct: 148 MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 207

Query: 61  IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR 120
           IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR
Sbjct: 208 IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR 267

Query: 121 KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 180
           KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG
Sbjct: 268 KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 327

Query: 181 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 240
           GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI
Sbjct: 328 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 387

Query: 241 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 300
           AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV
Sbjct: 388 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 447

Query: 301 SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD 360
           SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD
Sbjct: 448 SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD 507

Query: 361 KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT 420
           KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT
Sbjct: 508 KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT 567

Query: 421 SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR 480
           SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR
Sbjct: 568 SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR 627

Query: 481 IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK 540
           IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK
Sbjct: 628 IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK 687

Query: 541 ATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKV 600
           ATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKV
Sbjct: 688 ATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKV 747

Query: 601 TDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGE 660
           TDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGE
Sbjct: 748 TDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGE 807

Query: 661 EKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 707
           EKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN
Sbjct: 808 EKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 853

BLAST of MELO3C014075 vs. ExPASy TrEMBL
Match: A0A0A0L241 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045090 PE=3 SV=1)

HSP 1 Score: 1355.5 bits (3507), Expect = 0.0e+00
Identity = 664/707 (93.92%), Postives = 688/707 (97.31%), Query Frame = 0

Query: 1   MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 60
           MAEEAIFYSYTRSFNGFAA LDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND
Sbjct: 45  MAEEAIFYSYTRSFNGFAAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 104

Query: 61  IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR 120
           IGIPSNSIWN AKFGEDVI+ANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDS FHCNR
Sbjct: 105 IGIPSNSIWNTAKFGEDVIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNR 164

Query: 121 KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 180
           KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG
Sbjct: 165 KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 224

Query: 181 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 240
           GAPKARAVAYK CWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI
Sbjct: 225 GAPKARAVAYKACWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 284

Query: 241 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 300
           AAFLAVQRGILVVFSGGNSGPFPMT+ N+SPWVFTVAA+TIDR+F SYVGLGNKKH+KG+
Sbjct: 285 AAFLAVQRGILVVFSGGNSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGL 344

Query: 301 SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD 360
           SLSSV SLPKKF+PLINSVDAKF NVTEFHAQFCGKGTLDPMKVKGKIVICQVGET+GVD
Sbjct: 345 SLSSVPSLPKKFFPLINSVDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVD 404

Query: 361 KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT 420
           KG+QAS AGA GVI+AND+EKGDEI+PELHFIPASDITNTDAQIVQ YLKST+TP+AHLT
Sbjct: 405 KGFQASRAGAVGVIIANDLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLT 464

Query: 421 SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR 480
           SVKTLLSVKPAP IATFS+RGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFS  DRRR
Sbjct: 465 SVKTLLSVKPAPTIATFSARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRR 524

Query: 481 IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK 540
           IPFNVISGTSMSCPHVAGIAGL+KSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK
Sbjct: 525 IPFNVISGTSMSCPHVAGIAGLIKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK 584

Query: 541 ATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKV 600
           ATPYAYGAGQV PNDAADPGLVYDIT+NDYLNFLCARGY+AM+IKKFYAKPF+CVRSFKV
Sbjct: 585 ATPYAYGAGQVNPNDAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKV 644

Query: 601 TDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGE 660
           TDLNYPSISVGELKIGAPLT+NRRVKNVGSPGTYVARVKASPGVAV+IEPSTLVF  VGE
Sbjct: 645 TDLNYPSISVGELKIGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGE 704

Query: 661 EKGFKVVLQNTGKVKSG-DVFGTLIWSDGKHFVRSPIAVHLGPGIGN 707
           EKGFKVVLQNTGKVK+G DVFGTLIWSDGKHFVRS IAVHLGPGIGN
Sbjct: 705 EKGFKVVLQNTGKVKNGSDVFGTLIWSDGKHFVRSSIAVHLGPGIGN 751

BLAST of MELO3C014075 vs. ExPASy TrEMBL
Match: A0A5D3D763 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00150 PE=3 SV=1)

HSP 1 Score: 1345.5 bits (3481), Expect = 0.0e+00
Identity = 677/714 (94.82%), Postives = 682/714 (95.52%), Query Frame = 0

Query: 1   MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 60
           MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND
Sbjct: 71  MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 130

Query: 61  IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNR 120
           IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNF CNR
Sbjct: 131 IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNR 190

Query: 121 KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 180
           KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG
Sbjct: 191 KLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 250

Query: 181 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 240
           GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI
Sbjct: 251 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 310

Query: 241 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 300
           AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKH+K  
Sbjct: 311 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKVH 370

Query: 301 SLSSVASL-PKKFYP-------LINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQ 360
             + +  L   +F P       L N       +  E   QFCGKGTLDPMKVKGKIVICQ
Sbjct: 371 VYTHLLGLGASRFAPNFICAPELYNQY--SIGSTLETSNQFCGKGTLDPMKVKGKIVICQ 430

Query: 361 VGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKST 420
           VGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKST
Sbjct: 431 VGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKST 490

Query: 421 KTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPT 480
           KTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPT
Sbjct: 491 KTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPT 550

Query: 481 FSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQT 540
           FSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQT
Sbjct: 551 FSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQT 610

Query: 541 ILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPF 600
           ILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPF
Sbjct: 611 ILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPF 670

Query: 601 NCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPST 660
           NCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPST
Sbjct: 671 NCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPST 730

Query: 661 LVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 707
           LVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN
Sbjct: 731 LVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 782

BLAST of MELO3C014075 vs. ExPASy TrEMBL
Match: A0A6J1CQS4 (subtilisin-like protease SBT5.3 OS=Momordica charantia OX=3673 GN=LOC111013337 PE=3 SV=1)

HSP 1 Score: 1031.9 bits (2667), Expect = 1.2e-297
Identity = 499/704 (70.88%), Postives = 591/704 (83.95%), Query Frame = 0

Query: 1   MAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 60
           +AEE+IFYSYTRSFNGFAA LD+  A  LA+NP+VISVFEN+ARKLHTT SWNFLG+EN 
Sbjct: 69  IAEESIFYSYTRSFNGFAATLDENVAARLAKNPQVISVFENRARKLHTTHSWNFLGMENA 128

Query: 61  IGI-PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCN 120
            G+ P NSIWNAAKFG+D+I+ANIDTGVWPESKSFSDEG+GP+PS+W+G CQT  NFHCN
Sbjct: 129 QGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSRWKGTCQTYLNFHCN 188

Query: 121 RKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAK 180
           RKLIGGRYF+KGYEAAG KL+ + LTVRDHDGHGTHTL+TAAGNFV GANVFG GNGTAK
Sbjct: 189 RKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVFGQGNGTAK 248

Query: 181 GGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLA 240
           GGAP+AR  AYKVCWPPL + +CFDAD LA FEAAIADGVDV+STSLGG+  ++ +DPLA
Sbjct: 249 GGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVRDFADDPLA 308

Query: 241 IAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKG 300
           IAAF A+Q+GI+ VFS GN GP P +V+NV+PW FTVAA+TIDRDF SY+ LGNKK +KG
Sbjct: 309 IAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIALGNKKRIKG 368

Query: 301 VSLSSVASLPKKFYPLINSVDAKF--SNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETE 360
            SL+SVA LPKKFYPLI+SV  +    NVT++ AQFCG+GT DP KVKGKI++C  GE  
Sbjct: 369 ASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKILVCLEGEIV 428

Query: 361 GVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIA 420
           G +KG +A   GAAG+IV ND + GD+I+PELHF+PASD+  +D Q++ +Y+ ST+ P+ 
Sbjct: 429 GFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTQYMDSTRNPVG 488

Query: 421 HLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAAD 480
           HL  V+T L +KPAP++A FSSRGPN I+ +ILKPD+TAPGVNILA+Y +G APT S  D
Sbjct: 489 HLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGNAPTTSRYD 548

Query: 481 RRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDST 540
           RRRIPF+VISGTSMSCPH++GIA L+KSIHP WSPAAIKSAIMTTAKTR NN  +ILD T
Sbjct: 549 RRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNNLGSILDLT 608

Query: 541 KLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRS 600
           K+KATP+AYGAG V+PNDA DPGLVYD T+ DYLNFLCARGY A +I++FYAK + C RS
Sbjct: 609 KVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYAKSYVCDRS 668

Query: 601 FKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRS 660
           FK+TDLNYPSISV  L +G P+T+NRRVKNVGS GTYVARVK  P V++++EPSTL F S
Sbjct: 669 FKLTDLNYPSISVSNLTMG-PVTVNRRVKNVGSLGTYVARVKVHPEVSISVEPSTLQFSS 728

Query: 661 VGEEKGFKVVLQNTGKVK-SGDVFGTLIWSDGKHFVRSPIAVHL 701
           VGEEK FKVV Q  GK K  G +FGTLIWSDGKHFVRSPI ++L
Sbjct: 729 VGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNL 771

BLAST of MELO3C014075 vs. ExPASy TrEMBL
Match: A0A6J1L3L8 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498821 PE=3 SV=1)

HSP 1 Score: 979.2 bits (2530), Expect = 9.3e-282
Identity = 472/700 (67.43%), Postives = 573/700 (81.86%), Query Frame = 0

Query: 2   AEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDI 61
           A+E+I YSYTRSFNGFAAIL++KEA +LAR+P+VISV EN+ RKLHTT SW FLGVE+D 
Sbjct: 73  AKESILYSYTRSFNGFAAILNEKEAADLARSPQVISVLENRGRKLHTTNSWRFLGVEDDE 132

Query: 62  GIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNRK 121
           GIPSNSIWNAA+FG D+IIANIDTGVWPES SFSDEGYGP+PSKWRG C  D +F CNRK
Sbjct: 133 GIPSNSIWNAAQFGRDMIIANIDTGVWPESNSFSDEGYGPIPSKWRGTCPDDPSFRCNRK 192

Query: 122 LIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGG 181
           LIGGRYF+KGY+ AGG LNAT +++RDH+GHGTHTLSTAAGNFV GAN+FGHGNGTAKGG
Sbjct: 193 LIGGRYFYKGYKFAGGVLNATSVSIRDHNGHGTHTLSTAAGNFVPGANIFGHGNGTAKGG 252

Query: 182 APKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIA 241
           APKAR  AYK CWP + + +CFDAD+LAAFEAAI DGVDV+S SLGG   E+F DP+ IA
Sbjct: 253 APKARVAAYKACWPLIPEGECFDADVLAAFEAAINDGVDVISASLGGGDQEFFRDPVGIA 312

Query: 242 AFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGVS 301
           AF A Q+GI+V+FS GN GP P TV NV+PW  TVAA+T  R+FVS V LGN K +KG S
Sbjct: 313 AFHAAQQGIIVIFSAGNDGPQPETVGNVAPWEITVAASTTGRNFVSNVALGNNKILKGSS 372

Query: 302 LSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDK 361
           LSSV++LP +FYPLI+SV+AKFSNV+EF A+FCGKGTL+P KVKGKI+IC  G+  GV+K
Sbjct: 373 LSSVSALP-QFYPLIDSVNAKFSNVSEFQAKFCGKGTLNPKKVKGKILICTAGKITGVEK 432

Query: 362 GYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTS 421
           GY A+ AGA G+I+A +I+  +EI PEL+F+PAS IT +D +++  Y+ ST TP+A + +
Sbjct: 433 GYNAAEAGAVGMILATNIDNLEEIKPELYFLPASGITYSDGELLDDYINSTSTPVAQIMN 492

Query: 422 VKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRI 481
           V+T + + P+P++A FSSRGPNP D  ILKPD+TAPG  ILASYPT  APT S  D+RR 
Sbjct: 493 VRTEVEINPSPVVAAFSSRGPNPFDKAILKPDITAPGFTILASYPTTRAPTRSGFDQRRT 552

Query: 482 PFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKA 541
           PFNV SGTSM+CPH++ IA L+KSIHP WSPAAIKSA+MTTAKT  NNN        L A
Sbjct: 553 PFNVFSGTSMACPHISAIAALLKSIHPEWSPAAIKSALMTTAKTSDNNN------LLLNA 612

Query: 542 TPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVT 601
           TP+A+GAG V PNDA DPGLVYDIT+++YLNFLCARGY+A+++++F  +PF C +SFK  
Sbjct: 613 TPFAFGAGHVRPNDAMDPGLVYDITVDEYLNFLCARGYNAIQLRRFSKQPFVCEKSFKAI 672

Query: 602 DLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEE 661
           DLNYPSIS+  L + AP+TINRRVKNVG PGTYVARV+   GVA ++EPSTL F SVGEE
Sbjct: 673 DLNYPSISIPNLNVNAPVTINRRVKNVGGPGTYVARVELPEGVAASVEPSTLQFSSVGEE 732

Query: 662 KGFKVVLQNTGKVK-SGDVFGTLIWSDGKHFVRSPIAVHL 701
           K F+VV+QNTG++K  G VFG L+WSDGKH V SPI+++L
Sbjct: 733 KAFRVVVQNTGELKHEGYVFGKLVWSDGKHSVGSPISMNL 765

BLAST of MELO3C014075 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 783.9 bits (2023), Expect = 1.1e-226
Identity = 385/703 (54.77%), Postives = 512/703 (72.83%), Query Frame = 0

Query: 2   AEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDI 61
           A +AIFYSYT+  NGFAA LD   A  ++++P+V+SVF NKA KLHTTRSW+FLG+E++ 
Sbjct: 71  ATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNS 130

Query: 62  GIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQT--DSNFHCN 121
            +PS+SIW  A+FGED IIAN+DTGVWPESKSF DEG GP+PS+W+GICQ   D+ FHCN
Sbjct: 131 YVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCN 190

Query: 122 RKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAK 181
           RKLIG RYF+KGY AA G LN++  + RD DGHG+HTLSTAAG+FV G ++FG GNGTAK
Sbjct: 191 RKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAK 250

Query: 182 GGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLA 241
           GG+P+AR  AYKVCWPP+  ++C+DAD+LAAF+AAI DG DV+S SLGG    +FND +A
Sbjct: 251 GGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVA 310

Query: 242 IAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKG 301
           I +F A ++ I+VV S GNSGP   TV+NV+PW  TV A+T+DR+F S + LGN KH KG
Sbjct: 311 IGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKG 370

Query: 302 VSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGV 361
            SLSS A    KFYP++ SV+AK  N +   AQ C  G+LDP+K KGKI++C  G+   V
Sbjct: 371 QSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRV 430

Query: 362 DKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHL 421
           +KG   +L G  G+++ N    G+++  + H +PA+ +T+ D+  V +Y+  TK PIAH+
Sbjct: 431 EKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHI 490

Query: 422 TSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRR 481
           T  +T L +KPAP++A+FSS+GP+ +   ILKPD+TAPGV+++A+Y   ++PT    D R
Sbjct: 491 TPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPR 550

Query: 482 RIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKL 541
           R+ FN ISGTSMSCPH++GIAGL+K+ +P+WSPAAI+SAIMTTA    +    I ++T +
Sbjct: 551 RLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNM 610

Query: 542 KATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVR-SF 601
           KATP+++GAG V PN A +PGLVYD+ I DYLNFLC+ GY+A +I  F    F C     
Sbjct: 611 KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKI 670

Query: 602 KVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSV 661
            + +LNYPSI+V  L   + +T++R VKNVG P  Y  +V    GV V ++P++L F  V
Sbjct: 671 SLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKV 730

Query: 662 GEEKGFKVVL-QNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHL 701
           GE+K FKV+L ++ G V  G VFG L+WSD KH VRSPI V L
Sbjct: 731 GEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of MELO3C014075 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 766.5 bits (1978), Expect = 1.8e-221
Identity = 388/702 (55.27%), Postives = 494/702 (70.37%), Query Frame = 0

Query: 2   AEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDI 61
           A+EAIFYSY R  NGFAAILD+ EA  +A++P V+SVF NK RKLHTT SWNF+ +  + 
Sbjct: 81  AKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNG 140

Query: 62  GIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNRK 121
            +  +S+WN A +GED IIAN+DTGVWPESKSFSDEGYG VP++W+G C  D    CNRK
Sbjct: 141 VVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHKD--VPCNRK 200

Query: 122 LIGGRYFHKGYEA-AGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 181
           LIG RYF+KGY A  G   NA+  T RDHDGHG+HTLSTAAGNFV GANVFG GNGTA G
Sbjct: 201 LIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASG 260

Query: 182 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 241
           G+PKAR  AYKVCWPP+  ++CFDADILAA EAAI DGVDVLS S+GG A +Y +D +AI
Sbjct: 261 GSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAI 320

Query: 242 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 301
            +F AV+ G+ VV S GNSGP   TV+NV+PWV TV A+++DR+F ++V L N +  KG 
Sbjct: 321 GSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGT 380

Query: 302 SLSSVASLP-KKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGV 361
           SLS    LP +K Y LI++ DA  +N     A  C KG+LDP KVKGKI++C  G+   V
Sbjct: 381 SLSK--PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARV 440

Query: 362 DKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHL 421
           DKG QA+ AGAAG+++ ND   G+EI  + H +PAS I   D + +  YL STK P  ++
Sbjct: 441 DKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYI 500

Query: 422 TSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRR 481
            +    L+ KPAP +A+FSSRGPN I   ILKPD+TAPGVNI+A++     PT   +D R
Sbjct: 501 KAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNR 560

Query: 482 RIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKL 541
           R PFN  SGTSMSCPH++G+ GL+K++HP+WSPAAI+SAIMTT++TR N  + ++D +  
Sbjct: 561 RTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFK 620

Query: 542 KATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKP-FNCVRSF 601
           KA P++YG+G V PN AA PGLVYD+T  DYL+FLCA GY+   ++ F   P + C +  
Sbjct: 621 KANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGA 680

Query: 602 KVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSV 661
            + D NYPSI+V  L     +T+ R++KNVG P TY AR +   GV V++EP  L F   
Sbjct: 681 NLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKT 740

Query: 662 GEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHL 701
           GE K F++ L+      SG VFG L W+D  H+VRSPI V L
Sbjct: 741 GEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of MELO3C014075 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 605.5 bits (1560), Expect = 5.4e-173
Identity = 321/703 (45.66%), Postives = 450/703 (64.01%), Query Frame = 0

Query: 6   IFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPS 65
           + Y+Y  + +GF+  L  +EA++L   P VISV      +LHTTR+  FLG++      +
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----T 124

Query: 66  NSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFH---CNRKL 125
             ++  A    DV++  +DTGVWPESKS+SDEG+GP+PS W+G C+  +NF    CNRKL
Sbjct: 125 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 184

Query: 126 IGGRYFHKGYEAAGGKLNAT--LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 185
           IG R+F +GYE+  G ++ +    + RD DGHGTHT STAAG+ V GA++ G+ +GTA+G
Sbjct: 185 IGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARG 244

Query: 186 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 245
            AP+AR   YKVCW       CF +DILAA + AIAD V+VLS SLGG   +Y+ D +AI
Sbjct: 245 MAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAI 304

Query: 246 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGV 305
            AF A++RGILV  S GN+GP   +++NV+PW+ TV A T+DRDF +   LGN K+  GV
Sbjct: 305 GAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGV 364

Query: 306 SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD 365
           SL    +LP K  P I + +A  SN T  +   C  GTL P KVKGKIV+C  G    V 
Sbjct: 365 SLFKGEALPDKLLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 424

Query: 366 KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT 425
           KG     AG  G+I+AN    G+E+  + H +PA+ +      I++ Y+ +   P A ++
Sbjct: 425 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 484

Query: 426 SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR 485
            + T++ VKP+P++A FSSRGPN I   ILKPD+ APGVNILA++     PT  A+D RR
Sbjct: 485 ILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRR 544

Query: 486 IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILD-STKL 545
           + FN+ISGTSMSCPHV+G+A L+KS+HP WSPAAI+SA+MTTA     + + +LD +T  
Sbjct: 545 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 604

Query: 546 KATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNC--VRS 605
            +TP+ +GAG V P  A +PGL+YD+T  DYL FLCA  Y + +I+    + + C   +S
Sbjct: 605 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS 664

Query: 606 FKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKA-SPGVAVTIEPSTLVFR 665
           + V DLNYPS +V    +GA     R V +VG  GTY  +V + + GV +++EP+ L F+
Sbjct: 665 YSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFK 724

Query: 666 SVGEEKGFKVVLQ-NTGKVKSGDVFGTLIWSDGKHFVRSPIAV 699
              E+K + V    ++ K    + FG++ WSDGKH V SP+A+
Sbjct: 725 EANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of MELO3C014075 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 587.4 bits (1513), Expect = 1.5e-167
Identity = 324/717 (45.19%), Postives = 453/717 (63.18%), Query Frame = 0

Query: 3   EEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIG 62
           E  I + Y   F+GF+A++   EA+NL  +P V++VFE++ R+LHTTRS  FLG++N  G
Sbjct: 55  ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKG 114

Query: 63  IPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNF---HCN 122
                +W+ + +G DVII   DTG+WPE +SFSD   GP+P +WRG+C++ + F   +CN
Sbjct: 115 -----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCN 174

Query: 123 RKLIGGRYFHKGYEAAG-GKLNATL--LTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNG 182
           RK+IG R+F KG +AA  G +N T+  L+ RD DGHGTHT STAAG     A++ G+ +G
Sbjct: 175 RKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASG 234

Query: 183 TAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGA---ADEY 242
            AKG APKAR  AYKVCW    DS C D+DILAAF+AA+ DGVDV+S S+GG       Y
Sbjct: 235 VAKGVAPKARIAAYKVCWK---DSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPY 294

Query: 243 FNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGN 302
           + DP+AI ++ A  +GI V  S GN GP  M+VTN++PWV TV A+TIDR+F +   LG+
Sbjct: 295 YLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGD 354

Query: 303 KKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQV 362
              ++GVSL +   L  + +P++    +  S+     A  C + TLDP +V+GKIVIC  
Sbjct: 355 GHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSS-----ASLCMENTLDPKQVRGKIVICDR 414

Query: 363 GETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTK 422
           G +  V KG     AG  G+I+AN    G+ +  + H IPA  + + +   ++ Y  S  
Sbjct: 415 GSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHP 474

Query: 423 TPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTF 482
            PIA +    T++ +KPAP+IA+FS RGPN +   ILKPD+ APGVNILA++   + PT 
Sbjct: 475 NPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTG 534

Query: 483 SAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTI 542
             +D R+  FN++SGTSM+CPHV+G A L+KS HP+WSPA I+SA+MTT     N+N+++
Sbjct: 535 LPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSL 594

Query: 543 LD-STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPF 602
           +D ST   ATPY YG+G +    A +PGLVYDIT +DY+ FLC+ GY    I+     P 
Sbjct: 595 IDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPV 654

Query: 603 NCVRSFKVT--DLNYPSISV---GELKIGAPLTINRRVKNVG-SPGTYVARVKASPGVAV 662
            C  + K +  +LNYPSI+       +     T+ R   NVG +   Y AR+++  GV V
Sbjct: 655 RCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTV 714

Query: 663 TIEPSTLVFRSVGEEKGFKV-VLQNTGKV---KSGDVFGTLIWSD-GKHFVRSPIAV 699
           T++P  LVF S  + + + V V  NT  V   ++G VFG++ W D GKH VRSPI V
Sbjct: 715 TVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758

BLAST of MELO3C014075 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 571.6 bits (1472), Expect = 8.6e-163
Identity = 322/722 (44.60%), Postives = 436/722 (60.39%), Query Frame = 0

Query: 5   AIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIP 64
           +I ++Y   F+GF+A L  ++A  L  +P VISV   + R LHTTRS  FLG+ +     
Sbjct: 61  SIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRS---TD 120

Query: 65  SNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNF---HCNRK 124
              +   + FG D++I  IDTGVWPE  SF D G GPVP KW+G C    +F    CNRK
Sbjct: 121 KAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRK 180

Query: 125 LIGGRYFHKGYEAAGGKLNAT--LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAK 184
           L+G R+F  GYEA  GK+N T    + RD DGHGTHT S +AG +V  A+  G+ +G A 
Sbjct: 181 LVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAA 240

Query: 185 GGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLA 244
           G APKAR  AYKVCW    +S C+D+DILAAF+ A+ADGVDV+S S+GG    Y+ D +A
Sbjct: 241 GMAPKARLAAYKVCW----NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIA 300

Query: 245 IAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKG 304
           I AF A+ RGI V  S GN GP  +TVTNV+PW+ TV A TIDRDF + V LGN K + G
Sbjct: 301 IGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISG 360

Query: 305 VSLSSVASL-PKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEG 364
           VS+     L P + YPL+            + +  C +G+LDP  VKGKIV+C  G    
Sbjct: 361 VSVYGGPGLDPGRMYPLV--YGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSR 420

Query: 365 VDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYL------KST 424
             KG      G  G+I+AN +  G+ +  + H +PA+ +  +    +++Y+      +S+
Sbjct: 421 ATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSS 480

Query: 425 KTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPT 484
           K P A +    T L ++PAP++A+FS+RGPNP    ILKPDV APG+NILA++P  I P+
Sbjct: 481 KHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPS 540

Query: 485 FSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQT 544
              +D RR  FN++SGTSM+CPHV+G+A L+K+ HP+WSPAAI+SA++TTA T  N+ + 
Sbjct: 541 GVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEP 600

Query: 545 ILD-STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKP 604
           ++D ST   ++   YG+G V+P  A DPGLVYDIT  DY+NFLC   Y    I     + 
Sbjct: 601 MMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQ 660

Query: 605 FNC---VRSFKVTDLNYPSISV-----GELKIGAPLTINRRVKNVG-SPGTYVARVKASP 664
            +C    R+  V +LNYPS SV     GE K+       R V NVG S   Y  +++   
Sbjct: 661 ADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFI--RTVTNVGDSDSVYEIKIRPPR 720

Query: 665 GVAVTIEPSTLVFRSVGEEKGFKVVLQNTG-KVKSGDV---FGTLIWSDGKHFVRSPIAV 701
           G  VT+EP  L FR VG++  F V ++ T  K+  G      G ++WSDGK  V SP+ V
Sbjct: 721 GTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVV 771