MELO3C013735 (gene) Melon (DHL92) v4

BLAST of MELO3C013735 vs. NCBI nr
Match: XP_008448497.1 (PREDICTED: probable acyl-activating enzyme 2 [Cucumis melo])

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 541/541 (100.00%), Postives = 541/541 (100.00%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF
Sbjct: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS
Sbjct: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120

Query: 121 QFLPILLKSLENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELD 180
           QFLPILLKSLENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELD
Sbjct: 121 QFLPILLKSLENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELD 180

Query: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240
           PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF
Sbjct: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRR 300
           IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRR
Sbjct: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRR 300

Query: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLI 360
           VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLI
Sbjct: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLI 360

Query: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVG 420
           TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVG
Sbjct: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVG 420

Query: 421 VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLK 480
           VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLK
Sbjct: 421 VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLK 480

Query: 481 NGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNNNTI 540
           NGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNNNTI
Sbjct: 481 NGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNNNTI 540

Query: 541 T 542
           T
Sbjct: 541 T 541

BLAST of MELO3C013735 vs. NCBI nr
Match: XP_004146208.2 (probable acyl-activating enzyme 2 [Cucumis sativus] >KAE8649992.1 hypothetical protein Csa_010505 [Cucumis sativus])

HSP 1 Score: 1008.8 bits (2607), Expect = 1.7e-290
Identity = 500/542 (92.25%), Postives = 520/542 (95.94%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           MEGF+HCP NF PLSPVPFLKRASTLYGCRPSL+YG+R+F+WS TY R L++ASAL+HHF
Sbjct: 1   MEGFYHCPANFGPLSPVPFLKRASTLYGCRPSLVYGTRLFSWSDTYARSLALASALLHHF 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           HLSPADLVVAMAPNIPELYELHFAVPMAG IISALNTKLDSPTLSLLLQQLNPKVIF+DS
Sbjct: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDS 120

Query: 121 QFLPILLKSLENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELD 180
           QFLPILLKSLEN SIKFPAL+LIPSDP+T LPS+FLDYNK+L MR G D+FTPRPNAELD
Sbjct: 121 QFLPILLKSLENSSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELD 180

Query: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240
           PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF
Sbjct: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRR 300
           IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGP TLLKMIYESSSNNCMPRRLSRR
Sbjct: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRR 300

Query: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLI 360
           VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKP+FE +DNVQF+D LI
Sbjct: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFE-EDNVQFED-LI 360

Query: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVG 420
           TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKN+KATHEAF+GENWYRTGDVG
Sbjct: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVG 420

Query: 421 VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLK 480
           VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAE AVVGERTLYGFVKLK
Sbjct: 421 VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAEAAVVGERTLYGFVKLK 480

Query: 481 NGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALL-NGNNNT 540
           N SKEN DEIVEFCRMHLPEFM+PK IVFGDLPMNSTGKVQKF LREK KALL N NNNT
Sbjct: 481 NRSKENGDEIVEFCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVKALLNNANNNT 540

Query: 541 IT 542
           IT
Sbjct: 541 IT 540

BLAST of MELO3C013735 vs. NCBI nr
Match: KAA0052927.1 (putative acyl-activating enzyme 2 [Cucumis melo var. makuwa] >TYK11384.1 putative acyl-activating enzyme 2 [Cucumis melo var. makuwa])

HSP 1 Score: 944.1 bits (2439), Expect = 5.2e-271
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 71  MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 130
           MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 60

Query: 131 ENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYTSGS 190
           ENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYTSGS
Sbjct: 61  ENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYTSGS 120

Query: 191 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 250
           TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC
Sbjct: 121 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 180

Query: 251 NICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGALP 310
           NICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGALP
Sbjct: 181 NICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGALP 240

Query: 311 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDVKDP 370
           IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDVKDP
Sbjct: 241 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDVKDP 300

Query: 371 ISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGRIEM 430
           ISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGRIEM
Sbjct: 301 ISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGRIEM 360

Query: 431 KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLKNGSKENNDEI 490
           KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLKNGSKENNDEI
Sbjct: 361 KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLKNGSKENNDEI 420

Query: 491 VEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNNNTIT 542
           VEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNNNTIT
Sbjct: 421 VEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNNNTIT 471

BLAST of MELO3C013735 vs. NCBI nr
Match: XP_038903286.1 (probable acyl-activating enzyme 2 [Benincasa hispida])

HSP 1 Score: 909.4 bits (2349), Expect = 1.4e-260
Identity = 458/538 (85.13%), Postives = 491/538 (91.26%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           MEGF HCP NF PLSPV FLKRA+T+YG RPS++YG+RV+TWS+TYGRCL++ASALVHHF
Sbjct: 1   MEGFFHCPANFTPLSPVGFLKRAATVYGGRPSVVYGARVYTWSETYGRCLTLASALVHHF 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           H+SP DLVVAMAPNIPELYELHFAVPMAG IISALNTKLDS  LSLLLQQLNPK+IFLDS
Sbjct: 61  HVSPGDLVVAMAPNIPELYELHFAVPMAGAIISALNTKLDSSALSLLLQQLNPKIIFLDS 120

Query: 121 QFLPILLKSLENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDN-FTPRPNAEL 180
           QFLPILLKSLEN SIKFPAL+LI ++PNTS   ++ DYN++L MR   DN FTPR NAEL
Sbjct: 121 QFLPILLKSLENNSIKFPALVLISTNPNTS-RLEYFDYNRVLAMRFSDDNDFTPRSNAEL 180

Query: 181 DPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWC 240
           DPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRS IC STSSPVFLWTVDMFRCNGWC
Sbjct: 181 DPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSGICKSTSSPVFLWTVDMFRCNGWC 240

Query: 241 FIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSR 300
           FIW MAALGGCNICLRTVTA+AIFTNVELH+VTLLCGPSTLLKMI ES SN+ M RRLSR
Sbjct: 241 FIWAMAALGGCNICLRTVTAEAIFTNVELHKVTLLCGPSTLLKMISESLSNHYMSRRLSR 300

Query: 301 RVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHL 360
           RVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGP IIRPWKP+FE ++ VQF+D L
Sbjct: 301 RVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPVIIRPWKPNFE-EETVQFED-L 360

Query: 361 ITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDV 420
           ITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAF G+NWYRTGD+
Sbjct: 361 ITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFSGDNWYRTGDI 420

Query: 421 GVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKL 480
           G+RHKSGRIEMKDRAKDIVVR DGEG VSTVEVEGVLMSHP+VAE AVV ER L GFVK+
Sbjct: 421 GLRHKSGRIEMKDRAKDIVVRADGEGVVSTVEVEGVLMSHPDVAEAAVVEERMLCGFVKM 480

Query: 481 KNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNN 538
           K+  KEN DEIVEFCRM+LPEFMVPK IVFGDLPMNS GKVQKFV+REKAK  LN NN
Sbjct: 481 KDERKENGDEIVEFCRMYLPEFMVPKKIVFGDLPMNSIGKVQKFVVREKAKT-LNANN 534

BLAST of MELO3C013735 vs. NCBI nr
Match: XP_023007650.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 836.6 bits (2160), Expect = 1.2e-238
Identity = 429/544 (78.86%), Postives = 473/544 (86.95%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           M+ F   P NF PLSP+ F KRA+ +YG RPS++YGSRVFTWS+TYGRCL +ASALVHHF
Sbjct: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           H+SP D+V AMAPN+PELYELHFAVPMAG IIS LNTKLD+PTLSLLLQQL+PKVIFLDS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161

Query: 121 QFLPILLKSLENI---SIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNA 180
            FLP LL+SL      SI+FPAL+LIP+ P+T  PS+FLDYN++L MR   D FTPR N+
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTP-PSEFLDYNRVLEMRF--DEFTPRTNS 221

Query: 181 ELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNG 240
           ELD ISIN TSGSTGLHKGV+YSHRAAYLNSLATIFRS IC +TSSPVFLWTVDMFRCNG
Sbjct: 222 ELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNG 281

Query: 241 WCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSS-NNCMPRR 300
           WCFIW MAALGGCNICLR VT DAIFTN+ELH+VTLLCGPSTLLKMIYESSS NNC P  
Sbjct: 282 WCFIWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGF 341

Query: 301 LSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFD 360
           L RRVDLIVAGALPI EILTKV ELGFNISYGYGMTEAMGPA+IRPWKP+F D++ VQF+
Sbjct: 342 L-RRVDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNF-DEETVQFE 401

Query: 361 DHLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRT 420
           D LI SLEIDVKDP SMESVLGDGETLGEVMLRGN+LMSGYYKNLKAT EAF G+ WYRT
Sbjct: 402 D-LIASLEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGD-WYRT 461

Query: 421 GDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGF 480
           GDVGV+HKSGRIEMKDRAKDIVVR +GEGAVSTV+VE VLMSHP VAE AV+GER L G 
Sbjct: 462 GDVGVKHKSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVIGER-LCGL 521

Query: 481 VKLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGN 540
           VKLKNGS+ + +EIVEFCR HLPEFM+P+ +VFGDLPMNS GKVQKF+ REKAKAL    
Sbjct: 522 VKLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKAL--NT 575

BLAST of MELO3C013735 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 4.6e-129
Identity = 259/552 (46.92%), Postives = 352/552 (63.77%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           MEG   C  N  PLSP+ FL+R+S  Y    SL+YGS  +TW+QT+ RCL +ASAL  HF
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHF 88

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
            +SP D+V   + NIPE+YELHFAVPMAGGI+  LN + DS  +S LL     K+IF++ 
Sbjct: 89  GISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QFLPILLKSLENIS---IKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNF-TPRPN 180
           Q L     +L+ ++   IK P L+L+ +D  +   S +  YN +L    G D+F   RP 
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLL-TDSESFTSSSYDHYNHLLAN--GSDDFEIRRPK 208

Query: 181 AELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCN 240
            E DPISINYTSG+T   K V+YSHR AYLNS+AT+    +    +  V+LW+V MF CN
Sbjct: 209 NECDPISINYTSGTTARPKAVVYSHRGAYLNSIATVL---LHGMGTRSVYLWSVPMFHCN 268

Query: 241 GWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRR 300
           GWCF W  AA G  NIC+R V+  AIF N+ LH+VT      T+L MI  S   N +   
Sbjct: 269 GWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGN-LHTP 328

Query: 301 LSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPA---IIRPWKPSFEDQDNV 360
           L  +V+++  G+ P  +++ ++ E+GF +++ YG+TE  GPA   + +P   + + ++  
Sbjct: 329 LPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEERY 388

Query: 361 -----QFDDHLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAF 420
                Q  +HL    E+DV+DP+SMESV  DG T+GEVM RGNT+MSGY+K+LKAT EAF
Sbjct: 389 ALKARQGLNHLAME-EMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEAF 448

Query: 421 VGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVV 480
            G  W+RTGD+GV+H+ G I++KDR KD+V+   G   VSTVEVE VL SH  V E AVV
Sbjct: 449 EG-GWFRTGDLGVKHEDGYIQLKDRKKDVVI--SGGENVSTVEVETVLYSHEAVLEAAVV 508

Query: 481 GE------RTLYGFVKLKNG--SKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKV 533
                    T   FV LK G  +  + D+I++FCR  LP +M PKT+VF +LP  STGK+
Sbjct: 509 ARPDKLWGETPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKI 568

BLAST of MELO3C013735 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 3.9e-128
Identity = 264/561 (47.06%), Postives = 360/561 (64.17%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           +EG    P NF+PLSP+ FL+R++ +Y  R SL++GS   TW QTY RCL +ASAL  + 
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
            +S  D+V A+APN+P ++ELHFAVPMAG I+  LNT+LD  TLS+LL     K++F+D 
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167

Query: 121 QFLPILLKSLENI-----SIKFPALILI---------PSDPNTSLPSQF-LDYNKILTMR 180
           Q L I   +L+ +     + K   L+LI           D +++  S++  DY     ++
Sbjct: 168 QLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLK 227

Query: 181 LGVDNF-TPRPNAELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSP 240
            G   F   +P  E DPISINYTSG+T   KGV+YSHR AYLNSLAT+F  ++   +  P
Sbjct: 228 SGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM---SVYP 287

Query: 241 VFLWTVDMFRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMI 300
           V+LWTV MF CNGWC +W +AA GG NICLR V+   IF N+ +H+VT + G  T+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347

Query: 301 YESSSNNCMPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWK 360
              +     P  L  RV+++  G+ P+ +IL K+ ELGFN+S+ YG+TE  GP     WK
Sbjct: 348 VNYTVTEHKP--LPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWK 407

Query: 361 P-----SFEDQDNV---QFDDHLITSLE-IDVKDPISMESVLGDGETLGEVMLRGNTLMS 420
           P     S E++  +   Q   HL   LE +DVKDP++ME+V  DG T+GEVM RGNT+MS
Sbjct: 408 PEWDSLSLEERTKLKARQGVQHL--GLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMS 467

Query: 421 GYYKNLKATHEAFVGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGV 480
           GY+K+++AT +AF G+ W+ +GD+ V++  G IE+KDR KD+++   G   +S+VEVE V
Sbjct: 468 GYFKDIEATRKAFEGD-WFHSGDLAVKYPDGYIEIKDRLKDVII--SGGENISSVEVERV 527

Query: 481 LMSHPNVAETAVVGE------RTLYGFVKLKNG-SKENNDEIVEFCRMHLPEFMVPKTIV 530
           L SH  V E AVV        +T  GFVKLK G      +EI+ FCR HLP +M PKTIV
Sbjct: 528 LCSHQAVLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIV 587

BLAST of MELO3C013735 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 7.4e-127
Identity = 255/557 (45.78%), Postives = 347/557 (62.30%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           MEG    P N+ PL+P+ FL R++ +Y  R S++YGS  +TW QT  RC+ IASAL    
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
            +S  D+V  +APN+P + ELHF VPMAG ++  LN + DS  +++LL+    KVIF D 
Sbjct: 63  GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122

Query: 121 QFLPI---LLKSLENISIKFPALILIPSDPNTSL-----PSQFLDYNKILTMRLGVDNF- 180
           QFL I     + L N   K P L+LIP     S+       + ++Y  ++ M  G  +F 
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAM--GKSDFE 182

Query: 181 TPRPNAELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVD 240
             RP  E D IS+NYTSG+T   KGV+YSHR AYLNSLA +  +++    SSP +LWT  
Sbjct: 183 VIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEM---HSSPTYLWTNP 242

Query: 241 MFRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNN 300
           MF CNGWC +W + A+GG NICLR VTA AIF N+  H+VT + G  T+L MI  +  + 
Sbjct: 243 MFHCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESE 302

Query: 301 CMPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSF---- 360
             P  L  +V  I   A P   ++ K+ ELGF++ + YG+TE  GP  I  WKP +    
Sbjct: 303 QKP--LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLP 362

Query: 361 -EDQDNV---QFDDHLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKA 420
            E+Q  +   Q  +HL    EI VKDP++M ++  DG T+GEV+ RGNT+M+GY KN +A
Sbjct: 363 REEQAKMKARQGVNHLGLE-EIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEA 422

Query: 421 THEAFVGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVA 480
           T EAF G  W+ +GD+GV+H  G IE+KDR+KDI++   G   +S++EVE  L +HP V 
Sbjct: 423 TKEAFKG-GWFWSGDLGVKHPDGYIELKDRSKDIII--SGGENISSIEVESTLFTHPCVL 482

Query: 481 ETAVVGE------RTLYGFVKLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNST 535
           E AVV         T   FVKLK+GSK + +E++ +CR  LP +M P++IVF DLP  ST
Sbjct: 483 EAAVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTST 542

BLAST of MELO3C013735 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 7.4e-127
Identity = 253/552 (45.83%), Postives = 351/552 (63.59%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           MEG   C  N  PLSP+ FL+R+S  Y    SL+YGS  +TW+QT+ RCL +ASAL  H 
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHL 88

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
            +SP D+V   + N+PE+YELHFAVPMAGGI+  LN + DS  +S LL     K+IF++ 
Sbjct: 89  GISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QFLPILLKSLENIS---IKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNF-TPRPN 180
           Q L     +L+ ++   IK P L+L+ +D  +   S +  YN +L    G D+F   RP 
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLL-TDSESFTSSSYDHYNHLLAN--GSDDFEIRRPK 208

Query: 181 AELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCN 240
            E DPISINYTSG+T   K V+YSHR AYLNS+AT+    +    ++ V+LW+V MF CN
Sbjct: 209 NEWDPISINYTSGTTARPKAVVYSHRGAYLNSIATVL---LHGMGTTSVYLWSVPMFHCN 268

Query: 241 GWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRR 300
           GWCF W  AA G  NIC+R V+  AIF N+ LH+VT      T+L MI  S   N +   
Sbjct: 269 GWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGN-LHTP 328

Query: 301 LSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPA---IIRPWKPSFEDQDNV 360
           L  +V+++  G+ P  +++ ++ E+GF +++ YG+TE  GPA   + +P   + + ++  
Sbjct: 329 LPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERY 388

Query: 361 -----QFDDHLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAF 420
                Q  +HL    E+DV+DP++MESV  DG T+GEVM RGNT+MSGY+K+LKAT EAF
Sbjct: 389 ALKARQGLNHLAME-EMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEAF 448

Query: 421 VGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVV 480
            G  W+R+GD+GV+H+ G I++KDR KD+V+   G   +STVEVE VL SH  V E AVV
Sbjct: 449 EG-GWFRSGDLGVKHEDGYIQLKDRKKDVVI--SGGENISTVEVETVLYSHEAVLEAAVV 508

Query: 481 GE------RTLYGFVKLKNG--SKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKV 533
                    T   FV LK G  +  + D+I++FCR  LP +M PKT+VF +LP  STGK+
Sbjct: 509 ARPDKLWGETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKI 568

BLAST of MELO3C013735 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.6e-108
Identity = 222/560 (39.64%), Postives = 343/560 (61.25%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           M+    C  N  PL+P+ FLKRAS  Y  R S++YG   FTW QTY RC  +A++L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           +++  D+V  +APN+P +YE+HF+VPM G +++ +NT+LD+ T++++L+   PK++F+D 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 121 QFLPILLKSLENI----SIKFPALILIPSDPNTSLP-SQFLDYNKIL-----TMRLGVDN 180
           +F P++ + L  I    S   P +ILI    +T+ P S+ LDY  ++     T       
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASM 180

Query: 181 FTPRPNAELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTV 240
           F  R + E DPIS+NYTSG+T   KGV+ SH+ AYL++L++I   ++      PV+LWT+
Sbjct: 181 F--RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEM---GIFPVYLWTL 240

Query: 241 DMFRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSN 300
            MF CNGW   W +AA GG N+C+R VTA  I+ N+ELH VT +    T+ + + E S  
Sbjct: 241 PMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRT 300

Query: 301 NCMPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFE-- 360
           +  P+  S  V ++  G+ P   ++ KV +LGF++ +GYG+TEA GP +   W+  +   
Sbjct: 301 DQSPK--SSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKL 360

Query: 361 -DQDNVQFDDHL----ITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKA 420
            +   ++         +T  ++DVK+  ++ESV  DG+T+GE++++G++LM GY KN KA
Sbjct: 361 PEHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKA 420

Query: 421 THEAFVGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVA 480
           T EAF    W  TGD+GV H  G +E+KDR+KDI++   G   +S++EVE VL  +  V 
Sbjct: 421 TSEAF-KHGWLNTGDIGVIHPDGYVEIKDRSKDIII--SGGENISSIEVEKVLYMYQEVL 480

Query: 481 ETAVVG-ERTLYG-----FVKLKNGSK---ENNDEIVEFCRMHLPEFMVPKTIV-FGDLP 534
           E AVV     L+G     FV LK G +    +  +++++CR ++P FM PK +V F +LP
Sbjct: 481 EAAVVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELP 540

BLAST of MELO3C013735 vs. ExPASy TrEMBL
Match: A0A1S3BKP8 (probable acyl-activating enzyme 2 OS=Cucumis melo OX=3656 GN=LOC103490658 PE=4 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 541/541 (100.00%), Postives = 541/541 (100.00%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF
Sbjct: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS
Sbjct: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120

Query: 121 QFLPILLKSLENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELD 180
           QFLPILLKSLENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELD
Sbjct: 121 QFLPILLKSLENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELD 180

Query: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240
           PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF
Sbjct: 181 PISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRR 300
           IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRR
Sbjct: 241 IWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRR 300

Query: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLI 360
           VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLI
Sbjct: 301 VDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLI 360

Query: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVG 420
           TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVG
Sbjct: 361 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVG 420

Query: 421 VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLK 480
           VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLK
Sbjct: 421 VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLK 480

Query: 481 NGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNNNTI 540
           NGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNNNTI
Sbjct: 481 NGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNNNTI 540

Query: 541 T 542
           T
Sbjct: 541 T 541

BLAST of MELO3C013735 vs. ExPASy TrEMBL
Match: A0A5A7UEI8 (Putative acyl-activating enzyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00360 PE=4 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 2.5e-271
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 71  MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 130
           MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 60

Query: 131 ENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYTSGS 190
           ENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYTSGS
Sbjct: 61  ENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYTSGS 120

Query: 191 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 250
           TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC
Sbjct: 121 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 180

Query: 251 NICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGALP 310
           NICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGALP
Sbjct: 181 NICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGALP 240

Query: 311 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDVKDP 370
           IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDVKDP
Sbjct: 241 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDVKDP 300

Query: 371 ISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGRIEM 430
           ISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGRIEM
Sbjct: 301 ISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGRIEM 360

Query: 431 KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLKNGSKENNDEI 490
           KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLKNGSKENNDEI
Sbjct: 361 KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLKNGSKENNDEI 420

Query: 491 VEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNNNTIT 542
           VEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNNNTIT
Sbjct: 421 VEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNNNTIT 471

BLAST of MELO3C013735 vs. ExPASy TrEMBL
Match: A0A0A0L3L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002400 PE=4 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 4.1e-253
Identity = 443/472 (93.86%), Postives = 455/472 (96.40%), Query Frame = 0

Query: 71  MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 130
           MAPNIPELYELHFAVPMAG IISALNTKLDSPTLSLLLQQLNPKVIF+DSQFLPILLKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDSQFLPILLKSL 60

Query: 131 ENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYTSGS 190
           EN SIKFPAL+LIPSDP+T LPS+FLDYNK+L MR G D+FTPRPNAELDPISINYTSGS
Sbjct: 61  ENSSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINYTSGS 120

Query: 191 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 250
           TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC
Sbjct: 121 TGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALGGC 180

Query: 251 NICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGALP 310
           NICLRTVTADAIFTNVELHRVTLLCGP TLLKMIYESSSNNCMPRRLSRRVDLIVAGALP
Sbjct: 181 NICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRRVDLIVAGALP 240

Query: 311 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDVKDP 370
           IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKP+FE +DNVQF+D LITSLEIDVKDP
Sbjct: 241 IKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFE-EDNVQFED-LITSLEIDVKDP 300

Query: 371 ISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGRIEM 430
           ISMESVLGDGETLGEVMLRGNTLMSGYYKN+KATHEAF+GENWYRTGDVGVRHKSGRIEM
Sbjct: 301 ISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRIEM 360

Query: 431 KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLKNGSKENNDEI 490
           KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAE AVVGERTLYGFVKLKN SKEN DEI
Sbjct: 361 KDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAEAAVVGERTLYGFVKLKNRSKENGDEI 420

Query: 491 VEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALL-NGNNNTIT 542
           VEFCRMHLPEFM+PK IVFGDLPMNSTGKVQKF LREK KALL N NNNTIT
Sbjct: 421 VEFCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVKALLNNANNNTIT 470

BLAST of MELO3C013735 vs. ExPASy TrEMBL
Match: A0A6J1L3K3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111500216 PE=4 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 5.7e-239
Identity = 429/544 (78.86%), Postives = 473/544 (86.95%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           M+ F   P NF PLSP+ F KRA+ +YG RPS++YGSRVFTWS+TYGRCL +ASALVHHF
Sbjct: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           H+SP D+V AMAPN+PELYELHFAVPMAG IIS LNTKLD+PTLSLLLQQL+PKVIFLDS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161

Query: 121 QFLPILLKSLENI---SIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNA 180
            FLP LL+SL      SI+FPAL+LIP+ P+T  PS+FLDYN++L MR   D FTPR N+
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTP-PSEFLDYNRVLEMRF--DEFTPRTNS 221

Query: 181 ELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNG 240
           ELD ISIN TSGSTGLHKGV+YSHRAAYLNSLATIFRS IC +TSSPVFLWTVDMFRCNG
Sbjct: 222 ELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNG 281

Query: 241 WCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSS-NNCMPRR 300
           WCFIW MAALGGCNICLR VT DAIFTN+ELH+VTLLCGPSTLLKMIYESSS NNC P  
Sbjct: 282 WCFIWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGF 341

Query: 301 LSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFD 360
           L RRVDLIVAGALPI EILTKV ELGFNISYGYGMTEAMGPA+IRPWKP+F D++ VQF+
Sbjct: 342 L-RRVDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNF-DEETVQFE 401

Query: 361 DHLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRT 420
           D LI SLEIDVKDP SMESVLGDGETLGEVMLRGN+LMSGYYKNLKAT EAF G+ WYRT
Sbjct: 402 D-LIASLEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGD-WYRT 461

Query: 421 GDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGF 480
           GDVGV+HKSGRIEMKDRAKDIVVR +GEGAVSTV+VE VLMSHP VAE AV+GER L G 
Sbjct: 462 GDVGVKHKSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVIGER-LCGL 521

Query: 481 VKLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGN 540
           VKLKNGS+ + +EIVEFCR HLPEFM+P+ +VFGDLPMNS GKVQKF+ REKAKAL    
Sbjct: 522 VKLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKAL--NT 575

BLAST of MELO3C013735 vs. ExPASy TrEMBL
Match: A0A6J1E5I3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430963 PE=4 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 2.2e-238
Identity = 426/543 (78.45%), Postives = 474/543 (87.29%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           M+ F   P NF PLSPV FLK+++ +Y  RPS++YGSRVFTWS+TYGR L +ASALVHHF
Sbjct: 49  MDPFSPSPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 108

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           H+SP D+V AMAPN+PELYELHFAVPMAG IIS LNTKLD+PTLSLLLQQL+PK+IFLDS
Sbjct: 109 HVSPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 168

Query: 121 QFLPILLKSLENI---SIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNA 180
            FLP +L+SL      SI+FPAL+LIP+ P+T  PS+FLDYN++L MR   D+FTPR NA
Sbjct: 169 DFLPTVLQSLSQTYGNSIQFPALVLIPAHPDTP-PSEFLDYNRVLGMRF--DDFTPRTNA 228

Query: 181 ELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNG 240
           ELD ISIN TSGSTGLHKGV+YSHRAAYLNSLATIFRS IC +TSSPVFLWTVDMFRCNG
Sbjct: 229 ELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNG 288

Query: 241 WCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRL 300
           WCFIW MAALGGCNICLR VT DAIFTN+ELH+VTLLCGPSTLLKMIYESSS N     L
Sbjct: 289 WCFIWAMAALGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWL 348

Query: 301 SRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDD 360
           SRRVDLIVAGALPI EILTKV ELGFNISYGYGMTEAMGPA+IRPWKP+F D++ VQF+D
Sbjct: 349 SRRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNF-DEETVQFED 408

Query: 361 HLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTG 420
            LITSLEIDVKDP+SMESVLGDGETLGEVMLRGNTLMSGYYKNLKAT EAF G+ WYRTG
Sbjct: 409 -LITSLEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGD-WYRTG 468

Query: 421 DVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFV 480
           DVGVRHKSGRIEMKDRAKDIVVR +GE AVSTV+VE VLMSHP+VAE AVVGER L GFV
Sbjct: 469 DVGVRHKSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVVGER-LCGFV 528

Query: 481 KLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNN 540
           KLKNGS+ + +EIV+FCR HLPEFM+P+ +VFGDLPMNS GKVQKF+ REKAKAL    N
Sbjct: 529 KLKNGSRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKAL--NTN 582

BLAST of MELO3C013735 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 459.9 bits (1182), Expect = 2.8e-129
Identity = 264/561 (47.06%), Postives = 360/561 (64.17%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           +EG    P NF+PLSP+ FL+R++ +Y  R SL++GS   TW QTY RCL +ASAL  + 
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
            +S  D+V A+APN+P ++ELHFAVPMAG I+  LNT+LD  TLS+LL     K++F+D 
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167

Query: 121 QFLPILLKSLENI-----SIKFPALILI---------PSDPNTSLPSQF-LDYNKILTMR 180
           Q L I   +L+ +     + K   L+LI           D +++  S++  DY     ++
Sbjct: 168 QLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLK 227

Query: 181 LGVDNF-TPRPNAELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSP 240
            G   F   +P  E DPISINYTSG+T   KGV+YSHR AYLNSLAT+F  ++   +  P
Sbjct: 228 SGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM---SVYP 287

Query: 241 VFLWTVDMFRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMI 300
           V+LWTV MF CNGWC +W +AA GG NICLR V+   IF N+ +H+VT + G  T+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347

Query: 301 YESSSNNCMPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWK 360
              +     P  L  RV+++  G+ P+ +IL K+ ELGFN+S+ YG+TE  GP     WK
Sbjct: 348 VNYTVTEHKP--LPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWK 407

Query: 361 P-----SFEDQDNV---QFDDHLITSLE-IDVKDPISMESVLGDGETLGEVMLRGNTLMS 420
           P     S E++  +   Q   HL   LE +DVKDP++ME+V  DG T+GEVM RGNT+MS
Sbjct: 408 PEWDSLSLEERTKLKARQGVQHL--GLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMS 467

Query: 421 GYYKNLKATHEAFVGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGV 480
           GY+K+++AT +AF G+ W+ +GD+ V++  G IE+KDR KD+++   G   +S+VEVE V
Sbjct: 468 GYFKDIEATRKAFEGD-WFHSGDLAVKYPDGYIEIKDRLKDVII--SGGENISSVEVERV 527

Query: 481 LMSHPNVAETAVVGE------RTLYGFVKLKNG-SKENNDEIVEFCRMHLPEFMVPKTIV 530
           L SH  V E AVV        +T  GFVKLK G      +EI+ FCR HLP +M PKTIV
Sbjct: 528 LCSHQAVLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIV 587

BLAST of MELO3C013735 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 455.7 bits (1171), Expect = 5.3e-128
Identity = 255/557 (45.78%), Postives = 347/557 (62.30%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           MEG    P N+ PL+P+ FL R++ +Y  R S++YGS  +TW QT  RC+ IASAL    
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
            +S  D+V  +APN+P + ELHF VPMAG ++  LN + DS  +++LL+    KVIF D 
Sbjct: 63  GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122

Query: 121 QFLPI---LLKSLENISIKFPALILIPSDPNTSL-----PSQFLDYNKILTMRLGVDNF- 180
           QFL I     + L N   K P L+LIP     S+       + ++Y  ++ M  G  +F 
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAM--GKSDFE 182

Query: 181 TPRPNAELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVD 240
             RP  E D IS+NYTSG+T   KGV+YSHR AYLNSLA +  +++    SSP +LWT  
Sbjct: 183 VIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEM---HSSPTYLWTNP 242

Query: 241 MFRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNN 300
           MF CNGWC +W + A+GG NICLR VTA AIF N+  H+VT + G  T+L MI  +  + 
Sbjct: 243 MFHCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESE 302

Query: 301 CMPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSF---- 360
             P  L  +V  I   A P   ++ K+ ELGF++ + YG+TE  GP  I  WKP +    
Sbjct: 303 QKP--LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLP 362

Query: 361 -EDQDNV---QFDDHLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKA 420
            E+Q  +   Q  +HL    EI VKDP++M ++  DG T+GEV+ RGNT+M+GY KN +A
Sbjct: 363 REEQAKMKARQGVNHLGLE-EIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEA 422

Query: 421 THEAFVGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVA 480
           T EAF G  W+ +GD+GV+H  G IE+KDR+KDI++   G   +S++EVE  L +HP V 
Sbjct: 423 TKEAFKG-GWFWSGDLGVKHPDGYIELKDRSKDIII--SGGENISSIEVESTLFTHPCVL 482

Query: 481 ETAVVGE------RTLYGFVKLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNST 535
           E AVV         T   FVKLK+GSK + +E++ +CR  LP +M P++IVF DLP  ST
Sbjct: 483 EAAVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTST 542

BLAST of MELO3C013735 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 394.8 bits (1013), Expect = 1.1e-109
Identity = 222/560 (39.64%), Postives = 343/560 (61.25%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           M+    C  N  PL+P+ FLKRAS  Y  R S++YG   FTW QTY RC  +A++L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           +++  D+V  +APN+P +YE+HF+VPM G +++ +NT+LD+ T++++L+   PK++F+D 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 121 QFLPILLKSLENI----SIKFPALILIPSDPNTSLP-SQFLDYNKIL-----TMRLGVDN 180
           +F P++ + L  I    S   P +ILI    +T+ P S+ LDY  ++     T       
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASM 180

Query: 181 FTPRPNAELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTV 240
           F  R + E DPIS+NYTSG+T   KGV+ SH+ AYL++L++I   ++      PV+LWT+
Sbjct: 181 F--RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEM---GIFPVYLWTL 240

Query: 241 DMFRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSN 300
            MF CNGW   W +AA GG N+C+R VTA  I+ N+ELH VT +    T+ + + E S  
Sbjct: 241 PMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRT 300

Query: 301 NCMPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFE-- 360
           +  P+  S  V ++  G+ P   ++ KV +LGF++ +GYG+TEA GP +   W+  +   
Sbjct: 301 DQSPK--SSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKL 360

Query: 361 -DQDNVQFDDHL----ITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKA 420
            +   ++         +T  ++DVK+  ++ESV  DG+T+GE++++G++LM GY KN KA
Sbjct: 361 PEHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKA 420

Query: 421 THEAFVGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVA 480
           T EAF    W  TGD+GV H  G +E+KDR+KDI++   G   +S++EVE VL  +  V 
Sbjct: 421 TSEAF-KHGWLNTGDIGVIHPDGYVEIKDRSKDIII--SGGENISSIEVEKVLYMYQEVL 480

Query: 481 ETAVVG-ERTLYG-----FVKLKNGSK---ENNDEIVEFCRMHLPEFMVPKTIV-FGDLP 534
           E AVV     L+G     FV LK G +    +  +++++CR ++P FM PK +V F +LP
Sbjct: 481 EAAVVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELP 540

BLAST of MELO3C013735 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 388.3 bits (996), Expect = 1.0e-107
Identity = 220/480 (45.83%), Postives = 298/480 (62.08%), Query Frame = 0

Query: 78  LYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPI---LLKSLENIS 137
           + ELHF VPMAG ++  LN + DS  +++LL+    KVIF D QFL I     + L N  
Sbjct: 1   MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60

Query: 138 IKFPALILIPSDPNTSL-----PSQFLDYNKILTMRLGVDNF-TPRPNAELDPISINYTS 197
            K P L+LIP     S+       + ++Y  ++ M  G  +F   RP  E D IS+NYTS
Sbjct: 61  DKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAM--GKSDFEVIRPTDECDAISVNYTS 120

Query: 198 GSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAALG 257
           G+T   KGV+YSHR AYLNSLA +  +++    SSP +LWT  MF CNGWC +W + A+G
Sbjct: 121 GTTSSPKGVVYSHRGAYLNSLAAVLLNEM---HSSPTYLWTNPMFHCNGWCLLWGVTAIG 180

Query: 258 GCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAGA 317
           G NICLR VTA AIF N+  H+VT + G  T+L MI  +  +   P  L  +V  I   A
Sbjct: 181 GTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKP--LPGKVSFITGAA 240

Query: 318 LPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSF-----EDQDNV---QFDDHLI 377
            P   ++ K+ ELGF++ + YG+TE  GP  I  WKP +     E+Q  +   Q  +HL 
Sbjct: 241 PPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLG 300

Query: 378 TSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVG 437
              EI VKDP++M ++  DG T+GEV+ RGNT+M+GY KN +AT EAF G  W+ +GD+G
Sbjct: 301 LE-EIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKG-GWFWSGDLG 360

Query: 438 VRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGE------RTLY 497
           V+H  G IE+KDR+KDI++   G   +S++EVE  L +HP V E AVV         T  
Sbjct: 361 VKHPDGYIELKDRSKDIII--SGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETAC 420

Query: 498 GFVKLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLN 535
            FVKLK+GSK + +E++ +CR  LP +M P++IVF DLP  STGKVQKFVLR KAKAL++
Sbjct: 421 AFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKALVS 469

BLAST of MELO3C013735 vs. TAIR 10
Match: AT1G65890.1 (acyl activating enzyme 12 )

HSP 1 Score: 382.1 bits (980), Expect = 7.4e-106
Identity = 215/565 (38.05%), Postives = 329/565 (58.23%), Query Frame = 0

Query: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
           M+    C  N  PL+P+ FLKRAS  Y  R S++YG   FTW QTY RC  +A++L+   
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLI-SL 60

Query: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
           ++   D+V  +APN P +YE+HFAVPMAG +++ +NT+LD+ +++ +L+   PK++F+  
Sbjct: 61  NIGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYR 120

Query: 121 QFLPIL-----LKSLENISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRP 180
            F P+      L S E+ ++  P + +   D    + S+  DY  ++           R 
Sbjct: 121 SFEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEPTPLLLARM 180

Query: 181 ---NAELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDM 240
                E DPIS+NYTSG+T   KGV+ SHR AYL++L+ I   ++    + PV+LWT+ M
Sbjct: 181 FCIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEM---GTCPVYLWTLPM 240

Query: 241 FRCNGWCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNC 300
           F CNGW F W  AA GG ++C+R VTA  I+ N+E+H VT +C   T+  ++ + +S + 
Sbjct: 241 FHCNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDL 300

Query: 301 MPRRLSRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFED-QD 360
             R  S  V ++  G+ P   ++ KV  LGF + + YG+TEA GP +   W+  +    +
Sbjct: 301 SHR--SGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPE 360

Query: 361 NVQFD------DHLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATH 420
           N Q +        ++   E+DV++  + ESV  DG+T+GE++++G+++M GY KN KAT+
Sbjct: 361 NQQMELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATY 420

Query: 421 EAFVGENWYRTGDVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAET 480
           EAF    W  +GDVGV H  G +E+KDR+KDI++   G   +S+VEVE ++  +P V ET
Sbjct: 421 EAF-KHGWLNSGDVGVIHPDGHVEIKDRSKDIII--SGGENISSVEVENIIYKYPKVLET 480

Query: 481 AVVG------ERTLYGFVKLKNGSKENND----------EIVEFCRMHLPEFMVPKTIVF 534
           AVV         T   FV L+ G   N D          +++E+CR +LP FM P+ +VF
Sbjct: 481 AVVAMPHPTWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVF 540