MELO3C013323 (gene) Melon (DHL92) v4

BLAST of MELO3C013323 vs. NCBI nr
Match: XP_008448113.1 (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])

HSP 1 Score: 1854.7 bits (4803), Expect = 0.0e+00
Identity = 951/951 (100.00%), Postives = 951/951 (100.00%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVI 60
           MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVI
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVI 60

Query: 61  LKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDG 120
           LKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDG
Sbjct: 61  LKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDG 120

Query: 121 NEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAESPLS 180
           NEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAESPLS
Sbjct: 121 NEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAESPLS 180

Query: 181 QTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISG 240
           QTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISG
Sbjct: 181 QTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISG 240

Query: 241 VQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSGQLR 300
           VQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSGQLR
Sbjct: 241 VQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSGQLR 300

Query: 301 SGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKS 360
           SGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKS
Sbjct: 301 SGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKS 360

Query: 361 LWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLW 420
           LWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLW
Sbjct: 361 LWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLW 420

Query: 421 LGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVST 480
           LGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVST
Sbjct: 421 LGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVST 480

Query: 481 YFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFAS 540
           YFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFAS
Sbjct: 481 YFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFAS 540

Query: 541 IKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKRLLNIV 600
           IKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKRLLNIV
Sbjct: 541 IKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKRLLNIV 600

Query: 601 RYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMR 660
           RYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMR
Sbjct: 601 RYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMR 660

Query: 661 EDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVI 720
           EDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVI
Sbjct: 661 EDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVI 720

Query: 721 FGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDR 780
           FGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDR
Sbjct: 721 FGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDR 780

Query: 781 CEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIA 840
           CEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIA
Sbjct: 781 CEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIA 840

Query: 841 TPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERW 900
           TPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERW
Sbjct: 841 TPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERW 900

Query: 901 ARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 952
           ARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 ARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 951

BLAST of MELO3C013323 vs. NCBI nr
Match: KAA0040324.1 (mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa] >TYK23381.1 mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 1852.4 bits (4797), Expect = 0.0e+00
Identity = 950/951 (99.89%), Postives = 950/951 (99.89%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVI 60
           MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVI
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVI 60

Query: 61  LKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDG 120
           LKIDD GSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDG
Sbjct: 61  LKIDDSGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDG 120

Query: 121 NEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAESPLS 180
           NEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAESPLS
Sbjct: 121 NEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAESPLS 180

Query: 181 QTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISG 240
           QTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISG
Sbjct: 181 QTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISG 240

Query: 241 VQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSGQLR 300
           VQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSGQLR
Sbjct: 241 VQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSGQLR 300

Query: 301 SGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKS 360
           SGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKS
Sbjct: 301 SGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKS 360

Query: 361 LWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLW 420
           LWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLW
Sbjct: 361 LWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLW 420

Query: 421 LGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVST 480
           LGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVST
Sbjct: 421 LGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVST 480

Query: 481 YFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFAS 540
           YFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFAS
Sbjct: 481 YFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFAS 540

Query: 541 IKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKRLLNIV 600
           IKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKRLLNIV
Sbjct: 541 IKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKRLLNIV 600

Query: 601 RYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMR 660
           RYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMR
Sbjct: 601 RYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMR 660

Query: 661 EDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVI 720
           EDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVI
Sbjct: 661 EDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVI 720

Query: 721 FGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDR 780
           FGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDR
Sbjct: 721 FGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDR 780

Query: 781 CEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIA 840
           CEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIA
Sbjct: 781 CEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIA 840

Query: 841 TPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERW 900
           TPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERW
Sbjct: 841 TPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERW 900

Query: 901 ARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 952
           ARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 ARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 951

BLAST of MELO3C013323 vs. NCBI nr
Match: XP_004144925.1 (mechanosensitive ion channel protein 6 isoform X1 [Cucumis sativus] >KGN43281.1 hypothetical protein Csa_020565 [Cucumis sativus])

HSP 1 Score: 1696.4 bits (4392), Expect = 0.0e+00
Identity = 888/961 (92.40%), Postives = 902/961 (93.86%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDP 60
           MDTLKKSFKGNVSFKHTRKISAGG  SEINHEELPILLNHQ+TDH       H VNDSD 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SDRTEVILKIDDGG-SSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGAR 120
           SDRTEVILKID GG SSAVSRSLDS  NNGG VWRESRYDFWNNDE GIGES      AR
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGES------AR 120

Query: 121 MSD-SGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRII 180
           M D SG D NEGF+FVQTGYGMEDPPTKLIGDFL KQKI GETTLDMDLEMEELKPNRI+
Sbjct: 121 MGDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIV 180

Query: 181 PPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHE 240
           PPLAESPLSQTSKDLKVSFQ DSTEISSNDQS+RRR RDS+DLKEE KG Q P QQ HHE
Sbjct: 181 PPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHE 240

Query: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSG 300
           RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRL DPPAEPDRLSG
Sbjct: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSG 300

Query: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTL 360
           LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPD+FKRGNF+ALTVLQW SLI+ITAA ICTL
Sbjct: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTL 360

Query: 361 SVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGV 420
           SVPYLREKSLWELDIWKWEVMI ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGV
Sbjct: 361 SVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGV 420

Query: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480
           RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV
Sbjct: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480

Query: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIP 540
           LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG+ IP
Sbjct: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIP 540

Query: 541 PDLK-ATFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSA 600
           PDLK ATFASIKSGR I S RTHKSFCAKS K SRALTKN NDGITIDHLHKLS KNVSA
Sbjct: 541 PDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSA 600

Query: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYI 660
           WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVA RGYKYI
Sbjct: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYI 660

Query: 661 YLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAV 720
           YLDDL+RFMREDEVLKT SLFEGAAEN+RISKS LKNWVVN FRERRALALTLNDTKTAV
Sbjct: 661 YLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAV 720

Query: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780
           DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF
Sbjct: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780

Query: 781 VMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMG 840
           VMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMG
Sbjct: 781 VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG 840

Query: 841 ESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHR 900
           ES+EF VHIATPAEKITAMK RIISYIEGNKEHW PAPMIVFKDIDGLNK+KLAVWLSHR
Sbjct: 841 ESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHR 900

Query: 901 MNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 952
           MNHQDS ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA
Sbjct: 901 MNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 955

BLAST of MELO3C013323 vs. NCBI nr
Match: XP_038887578.1 (mechanosensitive ion channel protein 6-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1690.2 bits (4376), Expect = 0.0e+00
Identity = 871/959 (90.82%), Postives = 899/959 (93.74%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDP 60
           MDTLKKSFKGNVSFKHTRKISAGG  SEINHEELPILLNH+  DH       H VNDSD 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPADHRRRLRDRHPVNDSDS 60

Query: 61  SDRTEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARM 120
           SD TEVILKIDDGGSS VSRSLDSV +NGGKVWRESRY FWNN+  GIGESASRV GARM
Sbjct: 61  SDGTEVILKIDDGGSSTVSRSLDSVGSNGGKVWRESRYGFWNNNAIGIGESASRVSGARM 120

Query: 121 SDSGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPP 180
           SDSG D NEGF+FVQ GYGMEDPPTKLIG+FLHKQKIRGETTLDMDLEMEELKP+R + P
Sbjct: 121 SDSGVDRNEGFEFVQPGYGMEDPPTKLIGEFLHKQKIRGETTLDMDLEMEELKPDRSMTP 180

Query: 181 LAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERL 240
           L ESPL+QTSKDLKVSFQQDSTEISSNDQS+RRR RDS DL EEFKG QPPWQQSHHERL
Sbjct: 181 LPESPLNQTSKDLKVSFQQDSTEISSNDQSVRRRCRDSRDLPEEFKGGQPPWQQSHHERL 240

Query: 241 GSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLI 300
           GSPTISGVQNES AEAMR ASNLSFHSELSFQRKSNLLRAKTKSRL D PAEPDRLSGLI
Sbjct: 241 GSPTISGVQNESSAEAMRSASNLSFHSELSFQRKSNLLRAKTKSRLIDSPAEPDRLSGLI 300

Query: 301 PKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSV 360
           PKSGQLRSGFLGKI+DDDDDPFLE+DLPDDFKRGNF ALTVLQW SLILITAA +CTLS+
Sbjct: 301 PKSGQLRSGFLGKIDDDDDDPFLEEDLPDDFKRGNFNALTVLQWISLILITAALVCTLSI 360

Query: 361 PYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRK 420
           PYLREKSLWELDIWKWEVMILILICGRLVSGWGI+I VFFIERNFLLRK+VLYFVYGVRK
Sbjct: 361 PYLREKSLWELDIWKWEVMILILICGRLVSGWGIKIVVFFIERNFLLRKKVLYFVYGVRK 420

Query: 421 PVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLA 480
           PVQNCLWLGLVLIAWHLLFNKRVEKQTN SILNYV RVLVCLLIST +WLVKTLMVKVLA
Sbjct: 421 PVQNCLWLGLVLIAWHLLFNKRVEKQTNASILNYVRRVLVCLLISTFLWLVKTLMVKVLA 480

Query: 481 SSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPD 540
           SSFHVSTYFDRIQESLFNQYVIETLSGPPL+EI KNEEEEER+ADEVQKLQNAG+ IP D
Sbjct: 481 SSFHVSTYFDRIQESLFNQYVIETLSGPPLIEILKNEEEEERVADEVQKLQNAGINIPSD 540

Query: 541 LK-ATFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWN 600
           LK ATF+S+KSGR I S RTHKSFCAKSSK SRALTKNGNDGITIDHLHKLSPKNVSAWN
Sbjct: 541 LKAATFSSVKSGRVIGSGRTHKSFCAKSSKLSRALTKNGNDGITIDHLHKLSPKNVSAWN 600

Query: 601 MKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYL 660
           MKRLLNIVRYGSISTLDEQI+GP  DDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYL
Sbjct: 601 MKRLLNIVRYGSISTLDEQIQGPGRDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYL 660

Query: 661 DDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDK 720
           DDLMRFMREDEV KT SLFEGAAENRRISKS LKNWVVN FRERRALALTLNDTKTAVDK
Sbjct: 661 DDLMRFMREDEVFKTMSLFEGAAENRRISKSALKNWVVNAFRERRALALTLNDTKTAVDK 720

Query: 721 LHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVM 780
           LHHMVNVIF ILILILWLIVLGIASSKFF+FLSSQIVVVAFIFGNTCKTIFEAIIFLFVM
Sbjct: 721 LHHMVNVIFSILILILWLIVLGIASSKFFVFLSSQIVVVAFIFGNTCKTIFEAIIFLFVM 780

Query: 781 HPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGES 840
           HPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSP+MGES
Sbjct: 781 HPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPEMGES 840

Query: 841 VEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMN 900
           VEF+VHIATPAEKITAMKQRIISYIEGNKEHWCP+PM+VF DID LNKLKLAVWLSHRMN
Sbjct: 841 VEFYVHIATPAEKITAMKQRIISYIEGNKEHWCPSPMVVFMDIDDLNKLKLAVWLSHRMN 900

Query: 901 HQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 952
           HQD+ ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTS AS
Sbjct: 901 HQDAGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSSAS 959

BLAST of MELO3C013323 vs. NCBI nr
Match: XP_022135714.1 (mechanosensitive ion channel protein 6-like [Momordica charantia])

HSP 1 Score: 1486.1 bits (3846), Expect = 0.0e+00
Identity = 776/959 (80.92%), Postives = 844/959 (88.01%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHH------LVNDSDPS 60
           MDT+KKSFK NVSFKHTRKISAGG  +EI+H+ELPILL+H+    H       +ND DPS
Sbjct: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRHRGRRSMNDCDPS 60

Query: 61  DRTEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETG-----IGESASRVR 120
           DRTEVILKIDDGGS+AVSR    V  NGGKVWRE+ YDFWNND  G         ASRV 
Sbjct: 61  DRTEVILKIDDGGSAAVSR---PVEGNGGKVWRETNYDFWNNDGKGENGASRASGASRVS 120

Query: 121 GARMSDSGDDGNEGFQFVQTGYG--MEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKP 180
           GAR SD+G D NEGF+FVQ GYG  +EDPP KLIG+FLHKQK+RGETTLDMDLEMEEL+ 
Sbjct: 121 GARASDNG-DRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQ 180

Query: 181 NRIIPPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQ 240
           +RIIPPLAESPLSQTSKDLKVSFQQDSTE SSND S+RRRYRDS +L++E+KG+Q PWQQ
Sbjct: 181 DRIIPPLAESPLSQTSKDLKVSFQQDSTESSSND-SIRRRYRDSRELQDEYKGQQSPWQQ 240

Query: 241 SHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPD 300
           SHHER GSPTISGVQN+  AEA RC SNLSF  ELSFQR S+LLRAKTKSRL DPP EP 
Sbjct: 241 SHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPA 300

Query: 301 RLSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAF 360
            LSGLIPKSG LRSGFLGK E++DDDPFLE+DLP+DFKR +F+ LT+LQW SL+LIT A 
Sbjct: 301 LLSGLIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGAL 360

Query: 361 ICTLSVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYF 420
           +CTL +PYLR  SLWEL+IWKWEVM+LILICGRLVSGWGIRI VF IERNFLLRKR+LYF
Sbjct: 361 VCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYF 420

Query: 421 VYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTL 480
           VYGVRKPVQNC+WLGLVLIAW LLFNKRVE +TN ++L YV RVLV LL+STLIWLVKTL
Sbjct: 421 VYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTL 480

Query: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG 540
           MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPL+EIRKNEE EER+ADEVQKLQNAG
Sbjct: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAG 540

Query: 541 LTIPPDLKATFASIKSGRAISSERTHKSFCAKSS--KFSRALTKNGNDGITIDHLHKLSP 600
           ++IPPDLKA  +S+KSGR I      KS   KSS  K SR LTKNGNDGITIDHLHKLSP
Sbjct: 541 VSIPPDLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSP 600

Query: 601 KNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARR 660
           KNVSAWNMKRLLN+VRYGSISTLDEQI GPCL+DEST EI+SEREAKAAAKKIFQNVARR
Sbjct: 601 KNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARR 660

Query: 661 GYKYIYLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLND 720
             KYIY +DLMRFMREDEVL+T SLFEGA E+RRISKS LKNWVVN FRERR+LALTLND
Sbjct: 661 ELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLND 720

Query: 721 TKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEA 780
           TKTAVD+LHHMVNVIFGILILILWLI+LGIASSKFF F+SSQIVVVAFIFGNTCKTIFEA
Sbjct: 721 TKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEA 780

Query: 781 IIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYR 840
           IIFLFVMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYR
Sbjct: 781 IIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYR 840

Query: 841 SPDMGESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAV 900
           SPDMGESVEF +HIATPAE I  MKQRIISYIEGNK HW P+PM VFKD++ LN+L+LAV
Sbjct: 841 SPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAV 900

Query: 901 WLSHRMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPS 945
           WLSHRMNHQDS ERWARRSVLVE+VVKVCQELDIQYRLLPIDIN+ SLPSSAPS+GF S
Sbjct: 901 WLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFTS 954

BLAST of MELO3C013323 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 1.1e-274
Identity = 515/926 (55.62%), Postives = 653/926 (70.52%), Query Frame = 0

Query: 52  DPSDRTEVILKID--DGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGI--GESASR 111
           D +DR EVI+KID  +G ++ VS          GK+WR+  YDFW + E  +  G +A+ 
Sbjct: 4   DAADRREVIVKIDGENGNNNGVS------GETVGKIWRDGSYDFWTDGEGNLNKGHNAAA 63

Query: 112 VRGARMSDSGDD--GNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEEL 171
           V   R + +  +   +EGF+F +     EDPPTKLIG FLHKQ+  GE  LDMDL M+EL
Sbjct: 64  VDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMDEL 123

Query: 172 KPNRIIPPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPW 231
           + +R + P++ESP   T +D  V  +   +  ++ND                        
Sbjct: 124 Q-SRGLTPVSESPRVSTKRD-PVGRRDSRSNTNNNDD----------------------- 183

Query: 232 QQSHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSN-LLRAKTKSRLTDPPA 291
                                 E ++C+ N         QR S+ LL+ +T+SRL+DPP 
Sbjct: 184 ---------------------GEVVKCSGN-----NAPIQRSSSTLLKMRTRSRLSDPPT 243

Query: 292 ------EPDRLSGLIPKSGQLRSGFLGKI-----EDDDDDPFLEDDLPDDFKRGNFTALT 351
                   D  SG IPKSGQ++SGF GK      E+++DDPF  +DLP+++++   +   
Sbjct: 244 PQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWI 303

Query: 352 VLQWFSLILITAAFICTLSVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFF 411
           VL+W SLILI A F+CTL++P LR+K LWEL +WKWE M+L+LICGRLVS W ++I VFF
Sbjct: 304 VLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFF 363

Query: 412 IERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLV 471
           IERNFLLRKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+++V K  NT  L  V+++ V
Sbjct: 364 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFV 423

Query: 472 CLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEE 531
           CLL+  L+WLVKTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI+KNEEEE
Sbjct: 424 CLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEE 483

Query: 532 ERIADEVQKLQNAGLTIPPDLKATFASIKSGRAISSERTHKSFCAKSSKFSRALTKNG-- 591
           ERI+ EV+K QN G             I+SG   S  +T      KS   S  L+  G  
Sbjct: 484 ERISVEVKKFQNPG----------GVEIQSGAQKSPMKT-----GKSPFLSHVLSNGGGG 543

Query: 592 ---NDGITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSE 651
              N GITID LHKL+PKNVSAW MKRL+NI+R GS++TLDEQ++ P LDD+   +I+SE
Sbjct: 544 GGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSE 603

Query: 652 REAKAAAKKIFQNVARRGYKYIYLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNW 711
            EAK AA+KIF NVA+ G K+IY +D+MRF+ +DE LKT SLFEGA+E  RISKS LKNW
Sbjct: 604 FEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNW 663

Query: 712 VVNVFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQI 771
           VVN FRERRALALTLNDTKTAV++LH MVN++ GI+IL++WLI+LGI S+KF + +SSQ+
Sbjct: 664 VVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQV 723

Query: 772 VVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKI 831
           VVVAFIFGN CK +FE+II+LFV+HPFDVGDRCEIDG QMVVEEMNILTTVFLR+DN K+
Sbjct: 724 VVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKV 783

Query: 832 IIPNSVLATKLIHNFYRSPDMGESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAP 891
           + PNS+L TK I N+YRSPDMG+ +EF +HI TPAEKI  +KQRI SYIEG K+HW PAP
Sbjct: 784 VYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAP 843

Query: 892 MIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDI 951
           MIVFKD++ LN +++AVW +HRMNHQD  E+WARRS LVEE+ K+C+ELDI+YRL P+DI
Sbjct: 844 MIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDI 853

BLAST of MELO3C013323 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 895.6 bits (2313), Expect = 4.9e-259
Identity = 496/960 (51.67%), Postives = 664/960 (69.17%), Query Frame = 0

Query: 5   KKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVILKID 64
           + SFK + S+K   +I + G  SE + E LPIL +H      +V D    D T     +D
Sbjct: 4   RNSFKSHSSYK---QIRSPGDQSEPSPEHLPILHDHHPDHSGMVVDDQKPDSTR--SSLD 63

Query: 65  DGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGA-----RMSDSGDD 124
           DG ++ V               R++ Y FW ++ TG     + VR +      +S  GD 
Sbjct: 64  DGRNAPVE--------------RDASYKFWQDNTTGTSTDHTAVRTSDKDPIAISRKGDR 123

Query: 125 GNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRG----ETTLDMDLEMEELKPNRIIPPLA 184
            +  F FV     +++ PTK++      ++ RG    E TLD+D E +++    +  P +
Sbjct: 124 LSGSFDFVHGKLPVDESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDDVSHQTMPTPTS 183

Query: 185 ESPLS-QTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLG 244
            +  S   S++++VSF            ++RR           F     P   SH     
Sbjct: 184 TARTSFDASREMRVSF------------NVRR-------AGGAFVAGSVPSSSSHSSSSS 243

Query: 245 SPTISGVQNE---SLAEAMRCASNLSFHSELSFQRKSNLL-RAKTKSRLTDPPAEPDR-L 304
           S T+   Q++      E +RC SN      +SFQRKS L+ R KT+SRL DPP E +   
Sbjct: 244 SATMRTNQDQPQLQEEEVVRCTSN------MSFQRKSELISRVKTRSRLQDPPREEETPY 303

Query: 305 SGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFIC 364
           SG   +SGQL+SG L  I D++DDP  E+D+PD++KRG   A+T+LQW SL+ I AA  C
Sbjct: 304 SGW--RSGQLKSGLLADI-DEEDDPLAEEDVPDEYKRGKLDAITLLQWLSLVAIIAALAC 363

Query: 365 TLSVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVY 424
           +LS+   ++  +W L +WKWEV +L+LICGRLVSGWGIRI VFFIERNFLLRKRVLYFVY
Sbjct: 364 SLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVY 423

Query: 425 GVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMV 484
           GVR+ VQNCLWLGLVL+AWH LF+K+V+++T +  L YV+++LVC L+ST++WL+KTL+V
Sbjct: 424 GVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRFLPYVTKILVCFLLSTILWLIKTLVV 483

Query: 485 KVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLT 544
           KVLASSFHVSTYFDRIQE+LFNQYVIETLSGPP++E+ + EEEEER  DE+ K+QNAG  
Sbjct: 484 KVLASSFHVSTYFDRIQEALFNQYVIETLSGPPMIEMSRIEEEEERAQDEIFKMQNAGAN 543

Query: 545 IPPDL-KATFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGND-GITIDHLHKLSPKN 604
           +PPDL  A F   KSGR +            + K S  + K+  D GI+++HLH+++ KN
Sbjct: 544 LPPDLCAAAFPPGKSGRVM------------NPKLSPIIPKSTTDNGISMEHLHRMNHKN 603

Query: 605 VSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGY 664
           +SAWNMKRL+ IVR  S++TLDEQ+     +DEST +I+SE+EAKAAA+KIF+NV +RG 
Sbjct: 604 ISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSEKEAKAAARKIFKNVEQRGA 663

Query: 665 KYIYLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTK 724
           KYIYL+DLMRF+REDE +KT  LFEGA EN+RISKS LKNW+VN FRERRALALTLNDTK
Sbjct: 664 KYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWLVNAFRERRALALTLNDTK 723

Query: 725 TAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAII 784
           TAV+KLHHM+N++  I+I+++WL++L IASSK  +F+SSQ+V++AFIFGNT KT+FE+II
Sbjct: 724 TAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVVLLAFIFGNTVKTVFESII 783

Query: 785 FLFVMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSP 844
           FLF++HP+DVGDRCEID  Q+VVEEMNILTTVFLRYDNLKI+ PNS+L  K I+N+YRSP
Sbjct: 784 FLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIMYPNSLLWQKSINNYYRSP 843

Query: 845 DMGESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWL 904
           DMG+++EF VHI TP EKI+ +KQRI +YI+   E+W P   I+ KD++ L+ ++LA+W 
Sbjct: 844 DMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAKIIVKDLEDLHIVRLAIWP 903

Query: 905 SHRMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWT 948
            HR+NHQD AERW RR+VLVEEV+K+  ELDIQ+R  P+DIN+R++P+   S   P  W+
Sbjct: 904 CHRINHQDMAERWTRRAVLVEEVIKILLELDIQHRFYPLDINVRTMPTVVSS-RVPPGWS 903

BLAST of MELO3C013323 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 1.4e-253
Identity = 481/930 (51.72%), Postives = 634/930 (68.17%), Query Frame = 0

Query: 52  DPSDRTEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGA 111
           D +DR + I+ I+   S AV  +  S    GG +W+ES YDFW+ +         + +  
Sbjct: 5   DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGE---------KGKND 64

Query: 112 RMSDSGDDGNEGFQFVQTG------YGMEDPPTKLIGDFLHKQKIRG-ETTLDMDLEMEE 171
           +  D  D+    F F Q G        + DPP+KLIG FLHKQ+  G E +LD++L M E
Sbjct: 65  KKGDDEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAE 124

Query: 172 LKPNRIIPPLAESPLSQTSKDLKVSFQQDSTEISSN---DQSMRRRYRDSHDLKEEFKGE 231
           L+ N    P   +  S T +    +  + S+ + +    D   RR+ R S          
Sbjct: 125 LQSN---TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTS---------- 184

Query: 232 QPPWQQSHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTD 291
                      LG  +    +N   AE ++C S           +K  L R KTKSRL D
Sbjct: 185 -----------LGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQD 244

Query: 292 PPA-------EPDRLSGLIPKSGQLRSGFLGKI------------EDDDDDPFLEDDLPD 351
           PP        + +  SG   +SG  +SGFLGK             E++++DPFL++DLP+
Sbjct: 245 PPTPTHPAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPE 304

Query: 352 DFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKSLWELDIWKWEVMILILICGRLV 411
           +FKR   +    L+W SL+LI  + +C+L++  L+ K+ W+LD+WKWEV +L+LICGRLV
Sbjct: 305 EFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLV 364

Query: 412 SGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNT 471
           S W +RI VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T +
Sbjct: 365 SSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRS 424

Query: 472 SILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPP 531
           + L YV+RVLVCLL++ +IWLVKT++VKVLASSFH+STYFDRIQESLF QYVIETLSGPP
Sbjct: 425 TALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPP 484

Query: 532 LVEIRKNEEEEERIADEVQKLQN-AGLTIPPDLKATFASIKSGRAISSERTHKSFCAKSS 591
           L+EI++ EEEE+++A++V+ L+  AG  +PP LKAT  S                  KS 
Sbjct: 485 LMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMK-------------VGKSP 544

Query: 592 KFSRALTKNGND--GITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDD 651
             +R  +K G D  GI ID L +++ KNVSAWNMKRL+NI+  G+ISTLD+ ++    +D
Sbjct: 545 GLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQED 604

Query: 652 ESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMREDEVLKTTSLFEGAAENRR 711
           E  T I+SE EAK AA+KIF NV   G +YIYL+D +RF+ E+E  +  +LFEGA+E+ +
Sbjct: 605 EDATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDK 664

Query: 712 ISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSK 771
           ISKS LKNWVV  FRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIA+++
Sbjct: 665 ISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTR 724

Query: 772 FFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTTV 831
           F + LSSQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG Q+VVEEMNILTTV
Sbjct: 725 FLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTV 784

Query: 832 FLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIATPAEKITAMKQRIISYIEG 891
           FLRYDN KII PNSVL TK I N+YRSPDMG++VEF VHIATP EKITA+KQRI+SY++ 
Sbjct: 785 FLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDN 844

Query: 892 NKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWARRSVLVEEVVKVCQELDI 947
            K++W PAPMIVF  +D LN +K+AVWL+HRMNHQD  ER+ RR +L+EEV K C+ELDI
Sbjct: 845 KKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDI 875

BLAST of MELO3C013323 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 2.3e-240
Identity = 453/892 (50.78%), Postives = 624/892 (69.96%), Query Frame = 0

Query: 84  KVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDGNEGFQFVQTGYGME-DPPTKLIG 143
           K WRES  +FW+ND+      +S+  G       DDG+  F    +    E DPP+KLI 
Sbjct: 26  KFWRESSINFWHNDK------SSKPPGG----EEDDGSFDFMRRSSEKSEEPDPPSKLIN 85

Query: 144 DFLHKQKIRG-ETTLDMDLEMEELKPNRIIPPLAESPLSQTSKDLKVSFQQDSTEISSND 203
            FL+KQK  G E +LDM+  M EL+ N  +PPL+ + +S ++    V+    ++  +   
Sbjct: 86  QFLNKQKASGDEISLDMEANMPELQKN-TVPPLSSTAVSGSAS--PVTAPVTASYRNGTG 145

Query: 204 QSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISGVQNESLAEAMRCASNLSFHSE 263
            ++RRR ++   L    K       + +        + G      +E ++C SN      
Sbjct: 146 DAIRRR-QNRVTLSPSVKDGDSSEDEENR-------VDG------SEVVKCTSN------ 205

Query: 264 LSFQRKSNLLRAKTKSRLTDPPAE--PDRLSGLIPKSGQLRSGFLG-------------K 323
            S  R   L++ KT+SRL DPP    PD +SG  P+SG L  GF G             K
Sbjct: 206 RSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSK 265

Query: 324 IEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKSLWELDI 383
             ++++DPF E+DLP+  ++       +++W  LILI A+ IC+L +PYLR K+LW+L +
Sbjct: 266 DLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLAL 325

Query: 384 WKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLI 443
           WKWEVM+L+LICGRLVS W +++ V+F+E NFL RK+VLYFVYG+RKPVQNCLWLGLVLI
Sbjct: 326 WKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLI 385

Query: 444 AWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQ 503
           AWH LF+K+VE++  +++L YV++VL+CLL++ +IWL+KTL+VKVLASSFH+STYFDRIQ
Sbjct: 386 AWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQ 445

Query: 504 ESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFASIKSGRA 563
           ESLF QYVIETLSGPP +EI     EEE++A++V+  +  G  + P L     S      
Sbjct: 446 ESLFTQYVIETLSGPPRIEI---HIEEEKVANDVKTFEIVGRKLSP-LGPKAVSSPPQVT 505

Query: 564 ISSERTHK--SFCAKSSKFSRALTK--NGNDGITIDHLHKLSPKNVSAWNMKRLLNIVRY 623
           + S R  K  S   KS   SR+ +K   G +GI IDHL +++ KNVSAW MK+L+N+++ 
Sbjct: 506 VGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKK 565

Query: 624 GSISTLDEQIRGPCL-DDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMRE 683
           G++STLDEQI+     +D+  T+I+SE EAK AA+KIFQNVA  G +YIY++D MRF+ E
Sbjct: 566 GTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSE 625

Query: 684 DEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVIF 743
           DE  +   LFEGA+E  +ISKS LKNWVVN FRERRALALTLNDTKTAV++LH +V+V+ 
Sbjct: 626 DESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDVLV 685

Query: 744 GILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRC 803
            I+ILI+WL++LGIA++KF + +SSQ+++V F+FGN+CKTIFEA+IF+FVMHPFDVGDRC
Sbjct: 686 SIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRC 745

Query: 804 EIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIAT 863
           EIDG QM+VEEMNILTTVFLR+DN KI+ PNS+L TK I N+YRSPDM +++EFFVHIAT
Sbjct: 746 EIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIAT 805

Query: 864 PAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWA 923
           P EK TA++QRI+SY++  K+HW P+PMIVF+D+ GLN +K+A+W +H+MNHQ+  ER+ 
Sbjct: 806 PPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERYV 865

Query: 924 RRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAP--SIGFPSNWTSPAS 952
           RR  L+EE+ ++C+ELDI+YRL P++IN++SLP++ P  S   P +W    S
Sbjct: 866 RRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPITSDRIPPSWNQQRS 880

BLAST of MELO3C013323 vs. ExPASy Swiss-Prot
Match: F4IME1 (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 PE=2 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 6.3e-222
Identity = 397/689 (57.62%), Postives = 527/689 (76.49%), Query Frame = 0

Query: 266 RKSNLL-RAKTKSRLTDPPAEPD-RLSGLIPKSGQLRSGFLGKIEDD---DDDPFLEDDL 325
           RK+ L+ RAK +SRL DPP E + + S  I  S QLRSG LG+  DD   +DD   E+D+
Sbjct: 161 RKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDV 220

Query: 326 PDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKSLWELDIWKWEVMILILICGR 385
           P ++++    A+T+LQW SLI +  A + +L +   R  +LW L +WKWEV++L+LICGR
Sbjct: 221 PVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGR 280

Query: 386 LVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQT 445
           LVSG GIRI VFFIERNFLLRKRVLYFVYGV+  VQNCLWLGLVL+AWH LF+K+VEK+T
Sbjct: 281 LVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKET 340

Query: 446 NTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 505
            + +L  +S++LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LF+ Y+IETLSG
Sbjct: 341 QSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSG 400

Query: 506 PPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDL-KATFASIKSGRAISSERTHKSFCAK 565
           PP++E+ + EEEE+R  DE+ K+Q  G  + P+L  A F   KSG  +            
Sbjct: 401 PPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM------------ 460

Query: 566 SSKFSRALTKNGND-GITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLD 625
           + KFS  + K G+D GIT+D LHK++ KNVSAWNMKRL+ IVR  S+STLDEQ      +
Sbjct: 461 NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 520

Query: 626 DESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMREDEVLKTTSLFEGAAENR 685
           DEST +I+SE+EAKAAA+KIF+NVA+ G K+IYL+DLMRF+R DE +KT  LFEGA   +
Sbjct: 521 DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 580

Query: 686 RISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASS 745
           +I+KS LKNW+VN FRERRALALTLNDTKTAV+KLHHM++ +  I+I+++WLI+L IA+S
Sbjct: 581 KITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATS 640

Query: 746 KFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTT 805
           K+ +FL+SQ+V++AF+FGN+ KT+FE+IIFLF++HP+DVGDR  ID  +MVVEEMNILTT
Sbjct: 641 KYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTT 700

Query: 806 VFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIATPAEKITAMKQRIISYIE 865
           VFLR DNLKI+ PN +L  K IHN+ RSPDMG+ V   VHI TP EKI A+KQRI SYI+
Sbjct: 701 VFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYID 760

Query: 866 GNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWARRSVLVEEVVKVCQELD 925
              E+W P   ++ KD++ LN +++A+WL H++NHQ+  ER+ RR++L+EEV+K+  ELD
Sbjct: 761 SKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELD 820

Query: 926 IQYRLLPIDINIRSLPSSAPSIGFPSNWT 948
           IQYR  P+DIN++++P+   S   P  W+
Sbjct: 821 IQYRFHPLDINVKTMPTVVSS-RVPPAWS 836

BLAST of MELO3C013323 vs. ExPASy TrEMBL
Match: A0A1S3BID3 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103490400 PE=3 SV=1)

HSP 1 Score: 1854.7 bits (4803), Expect = 0.0e+00
Identity = 951/951 (100.00%), Postives = 951/951 (100.00%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVI 60
           MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVI
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVI 60

Query: 61  LKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDG 120
           LKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDG
Sbjct: 61  LKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDG 120

Query: 121 NEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAESPLS 180
           NEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAESPLS
Sbjct: 121 NEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAESPLS 180

Query: 181 QTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISG 240
           QTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISG
Sbjct: 181 QTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISG 240

Query: 241 VQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSGQLR 300
           VQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSGQLR
Sbjct: 241 VQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSGQLR 300

Query: 301 SGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKS 360
           SGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKS
Sbjct: 301 SGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKS 360

Query: 361 LWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLW 420
           LWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLW
Sbjct: 361 LWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLW 420

Query: 421 LGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVST 480
           LGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVST
Sbjct: 421 LGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVST 480

Query: 481 YFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFAS 540
           YFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFAS
Sbjct: 481 YFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFAS 540

Query: 541 IKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKRLLNIV 600
           IKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKRLLNIV
Sbjct: 541 IKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKRLLNIV 600

Query: 601 RYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMR 660
           RYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMR
Sbjct: 601 RYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMR 660

Query: 661 EDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVI 720
           EDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVI
Sbjct: 661 EDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVI 720

Query: 721 FGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDR 780
           FGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDR
Sbjct: 721 FGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDR 780

Query: 781 CEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIA 840
           CEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIA
Sbjct: 781 CEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIA 840

Query: 841 TPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERW 900
           TPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERW
Sbjct: 841 TPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERW 900

Query: 901 ARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 952
           ARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 ARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 951

BLAST of MELO3C013323 vs. ExPASy TrEMBL
Match: A0A5D3DI81 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004470 PE=3 SV=1)

HSP 1 Score: 1852.4 bits (4797), Expect = 0.0e+00
Identity = 950/951 (99.89%), Postives = 950/951 (99.89%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVI 60
           MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVI
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHHLVNDSDPSDRTEVI 60

Query: 61  LKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDG 120
           LKIDD GSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDG
Sbjct: 61  LKIDDSGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDG 120

Query: 121 NEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAESPLS 180
           NEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAESPLS
Sbjct: 121 NEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAESPLS 180

Query: 181 QTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISG 240
           QTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISG
Sbjct: 181 QTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISG 240

Query: 241 VQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSGQLR 300
           VQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSGQLR
Sbjct: 241 VQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKSGQLR 300

Query: 301 SGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKS 360
           SGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKS
Sbjct: 301 SGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKS 360

Query: 361 LWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLW 420
           LWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLW
Sbjct: 361 LWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLW 420

Query: 421 LGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVST 480
           LGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVST
Sbjct: 421 LGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVST 480

Query: 481 YFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFAS 540
           YFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFAS
Sbjct: 481 YFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFAS 540

Query: 541 IKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKRLLNIV 600
           IKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKRLLNIV
Sbjct: 541 IKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKRLLNIV 600

Query: 601 RYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMR 660
           RYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMR
Sbjct: 601 RYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMR 660

Query: 661 EDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVI 720
           EDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVI
Sbjct: 661 EDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVI 720

Query: 721 FGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDR 780
           FGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDR
Sbjct: 721 FGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDR 780

Query: 781 CEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIA 840
           CEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIA
Sbjct: 781 CEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIA 840

Query: 841 TPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERW 900
           TPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERW
Sbjct: 841 TPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERW 900

Query: 901 ARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 952
           ARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 ARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 951

BLAST of MELO3C013323 vs. ExPASy TrEMBL
Match: A0A0A0K619 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_7G017150 PE=3 SV=1)

HSP 1 Score: 1696.4 bits (4392), Expect = 0.0e+00
Identity = 888/961 (92.40%), Postives = 902/961 (93.86%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDP 60
           MDTLKKSFKGNVSFKHTRKISAGG  SEINHEELPILLNHQ+TDH       H VNDSD 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SDRTEVILKIDDGG-SSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGAR 120
           SDRTEVILKID GG SSAVSRSLDS  NNGG VWRESRYDFWNNDE GIGES      AR
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGES------AR 120

Query: 121 MSD-SGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRII 180
           M D SG D NEGF+FVQTGYGMEDPPTKLIGDFL KQKI GETTLDMDLEMEELKPNRI+
Sbjct: 121 MGDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIV 180

Query: 181 PPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHE 240
           PPLAESPLSQTSKDLKVSFQ DSTEISSNDQS+RRR RDS+DLKEE KG Q P QQ HHE
Sbjct: 181 PPLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHE 240

Query: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSG 300
           RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRL DPPAEPDRLSG
Sbjct: 241 RLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSG 300

Query: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTL 360
           LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPD+FKRGNF+ALTVLQW SLI+ITAA ICTL
Sbjct: 301 LIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTL 360

Query: 361 SVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGV 420
           SVPYLREKSLWELDIWKWEVMI ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGV
Sbjct: 361 SVPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGV 420

Query: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480
           RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV
Sbjct: 421 RKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKV 480

Query: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIP 540
           LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG+ IP
Sbjct: 481 LASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIP 540

Query: 541 PDLK-ATFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSA 600
           PDLK ATFASIKSGR I S RTHKSFCAKS K SRALTKN NDGITIDHLHKLS KNVSA
Sbjct: 541 PDLKAATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSA 600

Query: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYI 660
           WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVA RGYKYI
Sbjct: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYI 660

Query: 661 YLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAV 720
           YLDDL+RFMREDEVLKT SLFEGAAEN+RISKS LKNWVVN FRERRALALTLNDTKTAV
Sbjct: 661 YLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAV 720

Query: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780
           DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF
Sbjct: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780

Query: 781 VMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMG 840
           VMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMG
Sbjct: 781 VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG 840

Query: 841 ESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHR 900
           ES+EF VHIATPAEKITAMK RIISYIEGNKEHW PAPMIVFKDIDGLNK+KLAVWLSHR
Sbjct: 841 ESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHR 900

Query: 901 MNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 952
           MNHQDS ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA
Sbjct: 901 MNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPA 955

BLAST of MELO3C013323 vs. ExPASy TrEMBL
Match: A0A6J1C288 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111007608 PE=3 SV=1)

HSP 1 Score: 1486.1 bits (3846), Expect = 0.0e+00
Identity = 776/959 (80.92%), Postives = 844/959 (88.01%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHH------LVNDSDPS 60
           MDT+KKSFK NVSFKHTRKISAGG  +EI+H+ELPILL+H+    H       +ND DPS
Sbjct: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRHRGRRSMNDCDPS 60

Query: 61  DRTEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETG-----IGESASRVR 120
           DRTEVILKIDDGGS+AVSR    V  NGGKVWRE+ YDFWNND  G         ASRV 
Sbjct: 61  DRTEVILKIDDGGSAAVSR---PVEGNGGKVWRETNYDFWNNDGKGENGASRASGASRVS 120

Query: 121 GARMSDSGDDGNEGFQFVQTGYG--MEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKP 180
           GAR SD+G D NEGF+FVQ GYG  +EDPP KLIG+FLHKQK+RGETTLDMDLEMEEL+ 
Sbjct: 121 GARASDNG-DRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQ 180

Query: 181 NRIIPPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQ 240
           +RIIPPLAESPLSQTSKDLKVSFQQDSTE SSND S+RRRYRDS +L++E+KG+Q PWQQ
Sbjct: 181 DRIIPPLAESPLSQTSKDLKVSFQQDSTESSSND-SIRRRYRDSRELQDEYKGQQSPWQQ 240

Query: 241 SHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPD 300
           SHHER GSPTISGVQN+  AEA RC SNLSF  ELSFQR S+LLRAKTKSRL DPP EP 
Sbjct: 241 SHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPA 300

Query: 301 RLSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAF 360
            LSGLIPKSG LRSGFLGK E++DDDPFLE+DLP+DFKR +F+ LT+LQW SL+LIT A 
Sbjct: 301 LLSGLIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGAL 360

Query: 361 ICTLSVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYF 420
           +CTL +PYLR  SLWEL+IWKWEVM+LILICGRLVSGWGIRI VF IERNFLLRKR+LYF
Sbjct: 361 VCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYF 420

Query: 421 VYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTL 480
           VYGVRKPVQNC+WLGLVLIAW LLFNKRVE +TN ++L YV RVLV LL+STLIWLVKTL
Sbjct: 421 VYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTL 480

Query: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG 540
           MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPL+EIRKNEE EER+ADEVQKLQNAG
Sbjct: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAG 540

Query: 541 LTIPPDLKATFASIKSGRAISSERTHKSFCAKSS--KFSRALTKNGNDGITIDHLHKLSP 600
           ++IPPDLKA  +S+KSGR I      KS   KSS  K SR LTKNGNDGITIDHLHKLSP
Sbjct: 541 VSIPPDLKADTSSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSP 600

Query: 601 KNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARR 660
           KNVSAWNMKRLLN+VRYGSISTLDEQI GPCL+DEST EI+SEREAKAAAKKIFQNVARR
Sbjct: 601 KNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARR 660

Query: 661 GYKYIYLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLND 720
             KYIY +DLMRFMREDEVL+T SLFEGA E+RRISKS LKNWVVN FRERR+LALTLND
Sbjct: 661 ELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLND 720

Query: 721 TKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEA 780
           TKTAVD+LHHMVNVIFGILILILWLI+LGIASSKFF F+SSQIVVVAFIFGNTCKTIFEA
Sbjct: 721 TKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFEA 780

Query: 781 IIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYR 840
           IIFLFVMHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYR
Sbjct: 781 IIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYR 840

Query: 841 SPDMGESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAV 900
           SPDMGESVEF +HIATPAE I  MKQRIISYIEGNK HW P+PM VFKD++ LN+L+LAV
Sbjct: 841 SPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLAV 900

Query: 901 WLSHRMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPS 945
           WLSHRMNHQDS ERWARRSVLVE+VVKVCQELDIQYRLLPIDIN+ SLPSSAPS+GF S
Sbjct: 901 WLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFTS 954

BLAST of MELO3C013323 vs. ExPASy TrEMBL
Match: A0A6J1EJZ4 (mechanosensitive ion channel protein 6-like OS=Cucurbita moschata OX=3662 GN=LOC111435046 PE=4 SV=1)

HSP 1 Score: 1315.4 bits (3403), Expect = 0.0e+00
Identity = 687/936 (73.40%), Postives = 791/936 (84.51%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHH-------LVNDSDP 60
           MD+ KKS K N  FKH+RKISAGG  SEIN EELPILL+HQ +D H         ND +P
Sbjct: 1   MDSFKKSLKHNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRPKVRRPANDGEP 60

Query: 61  SDRTEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFW-NNDETGIGESASRVRGAR 120
           S  +EVILK+DDGGSSAVSRS +S A  GGKVWRESRYDFW NND  G G SAS   GAR
Sbjct: 61  SGHSEVILKVDDGGSSAVSRSQES-AGYGGKVWRESRYDFWSNNDGIGTGGSASWASGAR 120

Query: 121 MSDSGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIP 180
            SDSG D NEGF+FV+ G G +DPPTKLIGDFLHKQK+RGETTLD+DLEMEEL+ +R   
Sbjct: 121 ASDSG-DRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKT 180

Query: 181 PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHER 240
           P+ +SPLS+ SKDLKVSFQ DSTEISSN +S+RRRY++  +++EE K  QP W QSHHE 
Sbjct: 181 PMMDSPLSRASKDLKVSFQHDSTEISSN-ESVRRRYKEFREMQEESKRGQPSWHQSHHEH 240

Query: 241 LGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGL 300
            GS +ISGVQN+S+AEAMR ASNLSF S LS  RKS+LL+AK KSRLTDPPAEPDRLSGL
Sbjct: 241 GGS-SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGL 300

Query: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLS 360
           + KSGQLRSGFLG+ +D++DDPFLE+D PDDF+RG   ALTVLQW SL LITAA +CTLS
Sbjct: 301 VNKSGQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLS 360

Query: 361 VPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVR 420
           + +LRE SLWE  IWKWEVM+L+LICGRLVSGWGI I VFFIERNFLLRK+VLYFVYGV+
Sbjct: 361 IRHLRENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGVK 420

Query: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480
           +PVQNCLWLGLVLIAWHLLF+KRV+ QTN+ IL+YV + LVCLL+ TLIWL KTLM+KVL
Sbjct: 421 RPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLGTLIWLAKTLMLKVL 480

Query: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPP 540
           ASSFHVSTYFDRIQESLFNQYVIETLSGPP +E++K+EEE+++IADEVQ+LQ+AG+ +PP
Sbjct: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPP 540

Query: 541 DLK-ATFASIKSG-RAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSA 600
           DL+ A  +SIKSG R  S+  + +S   K  K SR  TKNG++GI IDHLHKL+ KNVSA
Sbjct: 541 DLRAAALSSIKSGERVRSNVGSQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSA 600

Query: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDES-TTEIKSEREAKAAAKKIFQNVARRGYKY 660
           WNMKRLL IVRYGSI+TLDEQI+GP LDDES TTEIKSEREAKAAAKKIFQNVAR G+KY
Sbjct: 601 WNMKRLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGFKY 660

Query: 661 IYLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTA 720
           IYLDDLMRFM+E+EVLKT S FEGA E RRISKS LKNWVVN FRERRAL+LTLNDTKTA
Sbjct: 661 IYLDDLMRFMKEEEVLKTLSRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTA 720

Query: 721 VDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFL 780
           V++L  +VNVIF I IL+LW+I+LGIAS K  +++SSQIV+VAFIFGNT K IFEAIIFL
Sbjct: 721 VERLGLVVNVIFSIFILVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFL 780

Query: 781 FVMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDM 840
           FVMHPFDVGDRCEID  QMVVEEMNILTTVFLR+DNLKII+PNSVLATK+IHN YRSPDM
Sbjct: 781 FVMHPFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDM 840

Query: 841 GESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSH 900
           GE +E  VHI TP EKITAMKQRIIS+IE +KEHWCP+P+I+ KDID  +KL +++WLSH
Sbjct: 841 GEFIEICVHIMTPLEKITAMKQRIISFIEADKEHWCPSPIILMKDIDSADKLAMSIWLSH 900

Query: 901 RMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLP 926
            MNHQD  ERWARRSV+VEEV+K+CQE DI   L+P
Sbjct: 901 TMNHQDIRERWARRSVVVEEVLKICQEFDITPTLVP 932

BLAST of MELO3C013323 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 947.6 bits (2448), Expect = 7.8e-276
Identity = 515/926 (55.62%), Postives = 653/926 (70.52%), Query Frame = 0

Query: 52  DPSDRTEVILKID--DGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGI--GESASR 111
           D +DR EVI+KID  +G ++ VS          GK+WR+  YDFW + E  +  G +A+ 
Sbjct: 4   DAADRREVIVKIDGENGNNNGVS------GETVGKIWRDGSYDFWTDGEGNLNKGHNAAA 63

Query: 112 VRGARMSDSGDD--GNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEEL 171
           V   R + +  +   +EGF+F +     EDPPTKLIG FLHKQ+  GE  LDMDL M+EL
Sbjct: 64  VDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMDEL 123

Query: 172 KPNRIIPPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPW 231
           + +R + P++ESP   T +D  V  +   +  ++ND                        
Sbjct: 124 Q-SRGLTPVSESPRVSTKRD-PVGRRDSRSNTNNNDD----------------------- 183

Query: 232 QQSHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSN-LLRAKTKSRLTDPPA 291
                                 E ++C+ N         QR S+ LL+ +T+SRL+DPP 
Sbjct: 184 ---------------------GEVVKCSGN-----NAPIQRSSSTLLKMRTRSRLSDPPT 243

Query: 292 ------EPDRLSGLIPKSGQLRSGFLGKI-----EDDDDDPFLEDDLPDDFKRGNFTALT 351
                   D  SG IPKSGQ++SGF GK      E+++DDPF  +DLP+++++   +   
Sbjct: 244 PQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWI 303

Query: 352 VLQWFSLILITAAFICTLSVPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFF 411
           VL+W SLILI A F+CTL++P LR+K LWEL +WKWE M+L+LICGRLVS W ++I VFF
Sbjct: 304 VLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFF 363

Query: 412 IERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLV 471
           IERNFLLRKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+++V K  NT  L  V+++ V
Sbjct: 364 IERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFV 423

Query: 472 CLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEE 531
           CLL+  L+WLVKTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI+KNEEEE
Sbjct: 424 CLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEE 483

Query: 532 ERIADEVQKLQNAGLTIPPDLKATFASIKSGRAISSERTHKSFCAKSSKFSRALTKNG-- 591
           ERI+ EV+K QN G             I+SG   S  +T      KS   S  L+  G  
Sbjct: 484 ERISVEVKKFQNPG----------GVEIQSGAQKSPMKT-----GKSPFLSHVLSNGGGG 543

Query: 592 ---NDGITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSE 651
              N GITID LHKL+PKNVSAW MKRL+NI+R GS++TLDEQ++ P LDD+   +I+SE
Sbjct: 544 GGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSE 603

Query: 652 REAKAAAKKIFQNVARRGYKYIYLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNW 711
            EAK AA+KIF NVA+ G K+IY +D+MRF+ +DE LKT SLFEGA+E  RISKS LKNW
Sbjct: 604 FEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNW 663

Query: 712 VVNVFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQI 771
           VVN FRERRALALTLNDTKTAV++LH MVN++ GI+IL++WLI+LGI S+KF + +SSQ+
Sbjct: 664 VVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQV 723

Query: 772 VVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKI 831
           VVVAFIFGN CK +FE+II+LFV+HPFDVGDRCEIDG QMVVEEMNILTTVFLR+DN K+
Sbjct: 724 VVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKV 783

Query: 832 IIPNSVLATKLIHNFYRSPDMGESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAP 891
           + PNS+L TK I N+YRSPDMG+ +EF +HI TPAEKI  +KQRI SYIEG K+HW PAP
Sbjct: 784 VYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAP 843

Query: 892 MIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDI 951
           MIVFKD++ LN +++AVW +HRMNHQD  E+WARRS LVEE+ K+C+ELDI+YRL P+DI
Sbjct: 844 MIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDI 853

BLAST of MELO3C013323 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 877.5 bits (2266), Expect = 9.9e-255
Identity = 481/930 (51.72%), Postives = 634/930 (68.17%), Query Frame = 0

Query: 52  DPSDRTEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGA 111
           D +DR + I+ I+   S AV  +  S    GG +W+ES YDFW+ +         + +  
Sbjct: 5   DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGE---------KGKND 64

Query: 112 RMSDSGDDGNEGFQFVQTG------YGMEDPPTKLIGDFLHKQKIRG-ETTLDMDLEMEE 171
           +  D  D+    F F Q G        + DPP+KLIG FLHKQ+  G E +LD++L M E
Sbjct: 65  KKGDDEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAE 124

Query: 172 LKPNRIIPPLAESPLSQTSKDLKVSFQQDSTEISSN---DQSMRRRYRDSHDLKEEFKGE 231
           L+ N    P   +  S T +    +  + S+ + +    D   RR+ R S          
Sbjct: 125 LQSN---TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTS---------- 184

Query: 232 QPPWQQSHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTD 291
                      LG  +    +N   AE ++C S           +K  L R KTKSRL D
Sbjct: 185 -----------LGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQD 244

Query: 292 PPA-------EPDRLSGLIPKSGQLRSGFLGKI------------EDDDDDPFLEDDLPD 351
           PP        + +  SG   +SG  +SGFLGK             E++++DPFL++DLP+
Sbjct: 245 PPTPTHPAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPE 304

Query: 352 DFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKSLWELDIWKWEVMILILICGRLV 411
           +FKR   +    L+W SL+LI  + +C+L++  L+ K+ W+LD+WKWEV +L+LICGRLV
Sbjct: 305 EFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLV 364

Query: 412 SGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNT 471
           S W +RI VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T +
Sbjct: 365 SSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRS 424

Query: 472 SILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPP 531
           + L YV+RVLVCLL++ +IWLVKT++VKVLASSFH+STYFDRIQESLF QYVIETLSGPP
Sbjct: 425 TALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPP 484

Query: 532 LVEIRKNEEEEERIADEVQKLQN-AGLTIPPDLKATFASIKSGRAISSERTHKSFCAKSS 591
           L+EI++ EEEE+++A++V+ L+  AG  +PP LKAT  S                  KS 
Sbjct: 485 LMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMK-------------VGKSP 544

Query: 592 KFSRALTKNGND--GITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDD 651
             +R  +K G D  GI ID L +++ KNVSAWNMKRL+NI+  G+ISTLD+ ++    +D
Sbjct: 545 GLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQED 604

Query: 652 ESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMREDEVLKTTSLFEGAAENRR 711
           E  T I+SE EAK AA+KIF NV   G +YIYL+D +RF+ E+E  +  +LFEGA+E+ +
Sbjct: 605 EDATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDK 664

Query: 712 ISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSK 771
           ISKS LKNWVV  FRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIA+++
Sbjct: 665 ISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTR 724

Query: 772 FFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTTV 831
           F + LSSQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG Q+VVEEMNILTTV
Sbjct: 725 FLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTV 784

Query: 832 FLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIATPAEKITAMKQRIISYIEG 891
           FLRYDN KII PNSVL TK I N+YRSPDMG++VEF VHIATP EKITA+KQRI+SY++ 
Sbjct: 785 FLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDN 844

Query: 892 NKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWARRSVLVEEVVKVCQELDI 947
            K++W PAPMIVF  +D LN +K+AVWL+HRMNHQD  ER+ RR +L+EEV K C+ELDI
Sbjct: 845 KKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDI 875

BLAST of MELO3C013323 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 833.6 bits (2152), Expect = 1.6e-241
Identity = 453/892 (50.78%), Postives = 624/892 (69.96%), Query Frame = 0

Query: 84  KVWRESRYDFWNNDETGIGESASRVRGARMSDSGDDGNEGFQFVQTGYGME-DPPTKLIG 143
           K WRES  +FW+ND+      +S+  G       DDG+  F    +    E DPP+KLI 
Sbjct: 26  KFWRESSINFWHNDK------SSKPPGG----EEDDGSFDFMRRSSEKSEEPDPPSKLIN 85

Query: 144 DFLHKQKIRG-ETTLDMDLEMEELKPNRIIPPLAESPLSQTSKDLKVSFQQDSTEISSND 203
            FL+KQK  G E +LDM+  M EL+ N  +PPL+ + +S ++    V+    ++  +   
Sbjct: 86  QFLNKQKASGDEISLDMEANMPELQKN-TVPPLSSTAVSGSAS--PVTAPVTASYRNGTG 145

Query: 204 QSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSPTISGVQNESLAEAMRCASNLSFHSE 263
            ++RRR ++   L    K       + +        + G      +E ++C SN      
Sbjct: 146 DAIRRR-QNRVTLSPSVKDGDSSEDEENR-------VDG------SEVVKCTSN------ 205

Query: 264 LSFQRKSNLLRAKTKSRLTDPPAE--PDRLSGLIPKSGQLRSGFLG-------------K 323
            S  R   L++ KT+SRL DPP    PD +SG  P+SG L  GF G             K
Sbjct: 206 RSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSK 265

Query: 324 IEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKSLWELDI 383
             ++++DPF E+DLP+  ++       +++W  LILI A+ IC+L +PYLR K+LW+L +
Sbjct: 266 DLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLAL 325

Query: 384 WKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLI 443
           WKWEVM+L+LICGRLVS W +++ V+F+E NFL RK+VLYFVYG+RKPVQNCLWLGLVLI
Sbjct: 326 WKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLI 385

Query: 444 AWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQ 503
           AWH LF+K+VE++  +++L YV++VL+CLL++ +IWL+KTL+VKVLASSFH+STYFDRIQ
Sbjct: 386 AWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQ 445

Query: 504 ESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLKATFASIKSGRA 563
           ESLF QYVIETLSGPP +EI     EEE++A++V+  +  G  + P L     S      
Sbjct: 446 ESLFTQYVIETLSGPPRIEI---HIEEEKVANDVKTFEIVGRKLSP-LGPKAVSSPPQVT 505

Query: 564 ISSERTHK--SFCAKSSKFSRALTK--NGNDGITIDHLHKLSPKNVSAWNMKRLLNIVRY 623
           + S R  K  S   KS   SR+ +K   G +GI IDHL +++ KNVSAW MK+L+N+++ 
Sbjct: 506 VGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKK 565

Query: 624 GSISTLDEQIRGPCL-DDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMRE 683
           G++STLDEQI+     +D+  T+I+SE EAK AA+KIFQNVA  G +YIY++D MRF+ E
Sbjct: 566 GTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSE 625

Query: 684 DEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVIF 743
           DE  +   LFEGA+E  +ISKS LKNWVVN FRERRALALTLNDTKTAV++LH +V+V+ 
Sbjct: 626 DESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDVLV 685

Query: 744 GILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRC 803
            I+ILI+WL++LGIA++KF + +SSQ+++V F+FGN+CKTIFEA+IF+FVMHPFDVGDRC
Sbjct: 686 SIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRC 745

Query: 804 EIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIAT 863
           EIDG QM+VEEMNILTTVFLR+DN KI+ PNS+L TK I N+YRSPDM +++EFFVHIAT
Sbjct: 746 EIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIAT 805

Query: 864 PAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWA 923
           P EK TA++QRI+SY++  K+HW P+PMIVF+D+ GLN +K+A+W +H+MNHQ+  ER+ 
Sbjct: 806 PPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERYV 865

Query: 924 RRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAP--SIGFPSNWTSPAS 952
           RR  L+EE+ ++C+ELDI+YRL P++IN++SLP++ P  S   P +W    S
Sbjct: 866 RRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPITSDRIPPSWNQQRS 880

BLAST of MELO3C013323 vs. TAIR 10
Match: AT3G14810.2 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 807.4 bits (2084), Expect = 1.3e-233
Identity = 455/930 (48.92%), Postives = 601/930 (64.62%), Query Frame = 0

Query: 52  DPSDRTEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGA 111
           D +DR + I+ I+   S AV  +  S    GG +W+ES YDFW+ +         + +  
Sbjct: 5   DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGE---------KGKND 64

Query: 112 RMSDSGDDGNEGFQFVQTG------YGMEDPPTKLIGDFLHKQKIRG-ETTLDMDLEMEE 171
           +  D  D+    F F Q G        + DPP+KLIG FLHKQ+  G E +LD++L M E
Sbjct: 65  KKGDDEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAE 124

Query: 172 LKPNRIIPPLAESPLSQTSKDLKVSFQQDSTEISSN---DQSMRRRYRDSHDLKEEFKGE 231
           L+ N    P   +  S T +    +  + S+ + +    D   RR+ R S          
Sbjct: 125 LQSN---TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTS---------- 184

Query: 232 QPPWQQSHHERLGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTD 291
                      LG  +    +N   AE ++C S           +K  L R KTKSRL D
Sbjct: 185 -----------LGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQD 244

Query: 292 PPA-------EPDRLSGLIPKSGQLRSGFLGKI------------EDDDDDPFLEDDLPD 351
           PP        + +  SG   +SG  +SGFLGK             E++++DPFL++DLP+
Sbjct: 245 PPTPTHPAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPE 304

Query: 352 DFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKSLWELDIWKWEVMILILICGRLV 411
           +FKR   +    L+W SL+LI  + +C+L++  L+ K+ W+LD+WKWEV +L+LICGRLV
Sbjct: 305 EFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLV 364

Query: 412 SGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNT 471
           S W +RI VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T +
Sbjct: 365 SSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRS 424

Query: 472 SILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPP 531
           + L                                  TYFDRIQESLF QYVIETLSGPP
Sbjct: 425 TALR---------------------------------TYFDRIQESLFTQYVIETLSGPP 484

Query: 532 LVEIRKNEEEEERIADEVQKLQN-AGLTIPPDLKATFASIKSGRAISSERTHKSFCAKSS 591
           L+EI++ EEEE+++A++V+ L+  AG  +PP LKAT  S                  KS 
Sbjct: 485 LMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMK-------------VGKSP 544

Query: 592 KFSRALTKNGND--GITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDD 651
             +R  +K G D  GI ID L +++ KNVSAWNMKRL+NI+  G+ISTLD+ ++    +D
Sbjct: 545 GLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQED 604

Query: 652 ESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMREDEVLKTTSLFEGAAENRR 711
           E  T I+SE EAK AA+KIF NV   G +YIYL+D +RF+ E+E  +  +LFEGA+E+ +
Sbjct: 605 EDATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDK 664

Query: 712 ISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSK 771
           ISKS LKNWV   FRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIA+++
Sbjct: 665 ISKSCLKNWV--AFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTR 724

Query: 772 FFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTTV 831
           F + LSSQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG Q+VVEEMNILTTV
Sbjct: 725 FLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTV 784

Query: 832 FLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIATPAEKITAMKQRIISYIEG 891
           FLRYDN KII PNSVL TK I N+YRSPDMG++VEF VHIATP EKITA+KQRI+SY++ 
Sbjct: 785 FLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDN 840

Query: 892 NKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWARRSVLVEEVVKVCQELDI 947
            K++W PAPMIVF  +D LN +K+AVWL+HRMNHQD  ER+ RR +L+EEV K C+ELDI
Sbjct: 845 KKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDI 840

BLAST of MELO3C013323 vs. TAIR 10
Match: AT2G17000.1 (Mechanosensitive ion channel family protein )

HSP 1 Score: 772.3 bits (1993), Expect = 4.5e-223
Identity = 397/689 (57.62%), Postives = 527/689 (76.49%), Query Frame = 0

Query: 266 RKSNLL-RAKTKSRLTDPPAEPD-RLSGLIPKSGQLRSGFLGKIEDD---DDDPFLEDDL 325
           RK+ L+ RAK +SRL DPP E + + S  I  S QLRSG LG+  DD   +DD   E+D+
Sbjct: 161 RKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDV 220

Query: 326 PDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYLREKSLWELDIWKWEVMILILICGR 385
           P ++++    A+T+LQW SLI +  A + +L +   R  +LW L +WKWEV++L+LICGR
Sbjct: 221 PVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGR 280

Query: 386 LVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQT 445
           LVSG GIRI VFFIERNFLLRKRVLYFVYGV+  VQNCLWLGLVL+AWH LF+K+VEK+T
Sbjct: 281 LVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKET 340

Query: 446 NTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 505
            + +L  +S++LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LF+ Y+IETLSG
Sbjct: 341 QSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSG 400

Query: 506 PPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDL-KATFASIKSGRAISSERTHKSFCAK 565
           PP++E+ + EEEE+R  DE+ K+Q  G  + P+L  A F   KSG  +            
Sbjct: 401 PPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM------------ 460

Query: 566 SSKFSRALTKNGND-GITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLD 625
           + KFS  + K G+D GIT+D LHK++ KNVSAWNMKRL+ IVR  S+STLDEQ      +
Sbjct: 461 NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 520

Query: 626 DESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLMRFMREDEVLKTTSLFEGAAENR 685
           DEST +I+SE+EAKAAA+KIF+NVA+ G K+IYL+DLMRF+R DE +KT  LFEGA   +
Sbjct: 521 DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 580

Query: 686 RISKSVLKNWVVNVFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASS 745
           +I+KS LKNW+VN FRERRALALTLNDTKTAV+KLHHM++ +  I+I+++WLI+L IA+S
Sbjct: 581 KITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATS 640

Query: 746 KFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGTQMVVEEMNILTT 805
           K+ +FL+SQ+V++AF+FGN+ KT+FE+IIFLF++HP+DVGDR  ID  +MVVEEMNILTT
Sbjct: 641 KYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTT 700

Query: 806 VFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIATPAEKITAMKQRIISYIE 865
           VFLR DNLKI+ PN +L  K IHN+ RSPDMG+ V   VHI TP EKI A+KQRI SYI+
Sbjct: 701 VFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYID 760

Query: 866 GNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWARRSVLVEEVVKVCQELD 925
              E+W P   ++ KD++ LN +++A+WL H++NHQ+  ER+ RR++L+EEV+K+  ELD
Sbjct: 761 SKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELD 820

Query: 926 IQYRLLPIDINIRSLPSSAPSIGFPSNWT 948
           IQYR  P+DIN++++P+   S   P  W+
Sbjct: 821 IQYRFHPLDINVKTMPTVVSS-RVPPAWS 836