MELO3C013315 (gene) Melon (DHL92) v4

BLAST of MELO3C013315 vs. NCBI nr
Match: KAA0040335.1 ((R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 573.5 bits (1477), Expect = 1.0e-159
Identity = 288/290 (99.31%), Postives = 288/290 (99.31%), Query Frame = 0

Query: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60
           MEETITKESKWK EEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ
Sbjct: 1   MEETITKESKWKNEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60

Query: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120
           LSTALNCSPP LFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE
Sbjct: 61  LSTALNCSPPHLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120

Query: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180
           IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV
Sbjct: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180

Query: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240
           ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG
Sbjct: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240

Query: 241 VEHVGGNMLDFVPKADAAFFMLKSLDQLVIEIVYITASGLLDADTLKSVK 291
           VEHVGGNMLDFVPKADAAFFMLKSLDQLVIEIVYITASGLLDADTLKSVK
Sbjct: 241 VEHVGGNMLDFVPKADAAFFMLKSLDQLVIEIVYITASGLLDADTLKSVK 290

BLAST of MELO3C013315 vs. NCBI nr
Match: XP_016900458.1 (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 532.7 bits (1371), Expect = 2.0e-147
Identity = 270/287 (94.08%), Postives = 272/287 (94.77%), Query Frame = 0

Query: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60
           MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ
Sbjct: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60

Query: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120
           LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE
Sbjct: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120

Query: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180
           IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV
Sbjct: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180

Query: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240
           ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG
Sbjct: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240

Query: 241 VEHVGGNMLDFVPKADAAFFMLKSLDQLVIEIVYITASGLLDADTLK 288
           VEHVGGNMLDFVPKADAAFFM+      VI I  I A G+LD    K
Sbjct: 241 VEHVGGNMLDFVPKADAAFFMVSMFINFVILI--IIALGVLDGTYTK 285

BLAST of MELO3C013315 vs. NCBI nr
Match: KAA0040332.1 ((R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK23368.1 (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK23371.1 (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 527.7 bits (1358), Expect = 6.3e-146
Identity = 261/261 (100.00%), Postives = 261/261 (100.00%), Query Frame = 0

Query: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60
           MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ
Sbjct: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60

Query: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120
           LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE
Sbjct: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120

Query: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180
           IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV
Sbjct: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180

Query: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240
           ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG
Sbjct: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240

Query: 241 VEHVGGNMLDFVPKADAAFFM 262
           VEHVGGNMLDFVPKADAAFFM
Sbjct: 241 VEHVGGNMLDFVPKADAAFFM 261

BLAST of MELO3C013315 vs. NCBI nr
Match: XP_004144926.1 (acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43288.1 hypothetical protein Csa_020491 [Cucumis sativus])

HSP 1 Score: 494.2 bits (1271), Expect = 7.7e-136
Identity = 246/261 (94.25%), Postives = 253/261 (96.93%), Query Frame = 0

Query: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60
           ME+TITKES WKK EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ
Sbjct: 1   MEQTITKESIWKK-EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60

Query: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120
           LST L CSPPLLFRIMRFLTHRGIF+EE TPENLIAYSQTPLSHMLINVAPLLLLENSPE
Sbjct: 61  LSTVLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPE 120

Query: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180
           IVASW NLSA+L+NSYNNN++SDL+VPFEVAHGKDIWSYGARNPGFNRMFNEGL CNARV
Sbjct: 121 IVASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARV 180

Query: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240
           ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVV TSEKYDG
Sbjct: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDG 240

Query: 241 VEHVGGNMLDFVPKADAAFFM 262
           VEHVGGNMLDFVPKADAAFFM
Sbjct: 241 VEHVGGNMLDFVPKADAAFFM 260

BLAST of MELO3C013315 vs. NCBI nr
Match: XP_038887461.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 439.5 bits (1129), Expect = 2.2e-119
Identity = 222/261 (85.06%), Postives = 235/261 (90.04%), Query Frame = 0

Query: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60
           MEETITKESKWKK EEEEAKIQIYKYVFGFAEAA IKCAIELKIA+ IESHGRP MTLSQ
Sbjct: 1   MEETITKESKWKK-EEEEAKIQIYKYVFGFAEAAAIKCAIELKIADAIESHGRP-MTLSQ 60

Query: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120
           LSTALNCSPPLLFRIMRFL HR IF+EETT EN+I YSQTPLSH+LI VAPLL+LENSP 
Sbjct: 61  LSTALNCSPPLLFRIMRFLVHRRIFKEETTAENVIGYSQTPLSHLLITVAPLLVLENSPT 120

Query: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180
           IV SWHNLSA+LK   NN+  SDL+ PFEVAHGK IWSY A NP FNRMFNEGLGCNARV
Sbjct: 121 IVESWHNLSARLK---NNDSTSDLLAPFEVAHGKGIWSYAATNPEFNRMFNEGLGCNARV 180

Query: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240
           ITLP ILE+CGDIFNG+ SLVDVGGGNGTTLS+LVKAFPWMKGINFDLPNVVSTSE+Y+G
Sbjct: 181 ITLPTILESCGDIFNGIGSLVDVGGGNGTTLSILVKAFPWMKGINFDLPNVVSTSEEYNG 240

Query: 241 VEHVGGNMLDFVPKADAAFFM 262
           VEHV GNML FVP ADAAFFM
Sbjct: 241 VEHVAGNMLHFVPNADAAFFM 256

BLAST of MELO3C013315 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.5e-57
Identity = 116/255 (45.49%), Postives = 165/255 (64.71%), Query Frame = 0

Query: 14  EEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESH-GRPTMTLSQLSTALNCSPPLL 73
           +EE +A + I+KYVFGFA+ A  KCAI+LKI   IE+H     +TL++LS+A++ SP  L
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHL 83

Query: 74  FRIMRFLTHRGIFREETTPENL-IAYSQTPLSHMLI-------NVAPLLLLENSPEIVAS 133
            RIMRFL H+GIF+E  T + L   Y  TPLS  L+       ++AP +L E +PE++A 
Sbjct: 84  RRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAP 143

Query: 134 WHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARVITLP 193
           W  LS+ + +  N +       PF+  HGKD+WS+   NP  + M NE + C+AR + +P
Sbjct: 144 WLRLSSVVSSPVNGSTPP----PFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRV-VP 203

Query: 194 AILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDGVEHV 253
            +   C  +F+GV ++VDVGGG G T+ +LVK FPW+KG NFDLP+V+  +E  DGVE+V
Sbjct: 204 RVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENV 263

Query: 254 GGNMLDFVPKADAAF 260
            G+M D +P  DA F
Sbjct: 264 EGDMFDSIPACDAIF 273

BLAST of MELO3C013315 vs. ExPASy Swiss-Prot
Match: B0ZB55 (Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 PE=1 SV=2)

HSP 1 Score: 167.5 bits (423), Expect = 2.2e-40
Identity = 97/252 (38.49%), Postives = 154/252 (61.11%), Query Frame = 0

Query: 22  QIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTAL------NCSP--PLLF 81
           QI++ +F F ++  +KCAIEL+IA++I SHG+P +TLSQ+++ +      + SP  P L 
Sbjct: 9   QIWQLMFSFVDSMALKCAIELRIADIIHSHGKP-ITLSQIASGIRSNSNSSISPNIPYLS 68

Query: 82  RIMRFLTHRGIFRE--ETTPENLIAYSQTPLSHMLI-----NVAPLLLLENSPEIVASWH 141
           RIMRFL  + IF E  E   E +  Y  +  S  L+     ++AP++L++  P  +A WH
Sbjct: 69  RIMRFLVRKNIFTEHQEDNDEVISLYGLSDSSRWLLRDFKSSLAPMVLMQTHPLSMAVWH 128

Query: 142 NLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARVITLPAI 201
            L   ++NS N          FE AHG +IW + + NP FN++FN  +  +   I + A+
Sbjct: 129 FLEDYVRNSSNT---------FEKAHGCNIWEFSSANPDFNKIFNNAM-ASIVPIYMGAV 188

Query: 202 LENCGDIFNGVE-SLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDGVEHVG 258
           L +  D    ++ ++VDVGGG G ++S L+K +P +KGINFDLP+V++T+   DGV H+ 
Sbjct: 189 LSSYKDGLGCIKGTVVDVGGGTGGSISELMKYYPNIKGINFDLPHVIATAPALDGVTHIS 248

BLAST of MELO3C013315 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.1e-39
Identity = 100/260 (38.46%), Postives = 154/260 (59.23%), Query Frame = 0

Query: 14  EEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTALNCSPP--- 73
           EE  + + +I++++F F ++  +KCA+EL I ++I SHGRP +T+S++  +L  + P   
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRP-VTISEIVDSLKTNTPSSS 64

Query: 74  ----LLFRIMRFLTHRGIFREETTPE-NLIAYSQTPLSHMLI-----NVAPLLLLENSPE 133
                L RIMR L H+ +F  E   E N + Y+ T  S  L+     N++PL+L E +P 
Sbjct: 65  PNIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPI 124

Query: 134 IVASWHNLS--AKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNA 193
           ++  W  L   A+ K+S           PFE AHG +IW     +P FN   N  + C+ 
Sbjct: 125 LLKPWQYLGKCAQEKSS-----------PFERAHGCEIWDLALADPKFNNFLNGAMQCST 184

Query: 194 RVITLPAILENCGDIFNGVE-SLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEK 253
             I    +LE   D F+G+  SLVDVGGG G+ ++ +VKA P ++GINFDLP+VV+T+ +
Sbjct: 185 TTIINEMLLE-YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAE 244

Query: 254 YDGVEHVGGNMLDFVPKADA 258
           + GV+HVGG+M   +P+ADA
Sbjct: 245 FPGVKHVGGDMFVDIPEADA 251

BLAST of MELO3C013315 vs. ExPASy Swiss-Prot
Match: Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 7.7e-38
Identity = 95/274 (34.67%), Postives = 159/274 (58.03%), Query Frame = 0

Query: 13  KEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTALNCSP--- 72
           K++   ++ +++ +++GFAE+ V+KCA++L +AN+I + G  +MTLS+LS+ L   P   
Sbjct: 4   KKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSG-TSMTLSELSSRLPSQPVNE 63

Query: 73  PLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLIN-----VAPLLLLENSPEIVAS 132
             L+R+MR+L H  +F + +  +  + Y   P +  L+      +   +L     + +A 
Sbjct: 64  DALYRVMRYLVHMKLFTKASI-DGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAP 123

Query: 133 WHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARVITLP 192
           WH L   L        + +    FE A G +IW Y A +P  N++FNE +  ++R+I + 
Sbjct: 124 WHYLKDGL--------SGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLI-MS 183

Query: 193 AILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDGVEHV 252
           A+++ CG+IFNG+ +LVDVGGG GT +  +  AFP +K   +DLP+V++ S  Y  V  V
Sbjct: 184 ALVKECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCV 243

Query: 253 GGNMLDFVPKADAAFFMLKSL-----DQLVIEIV 274
            G+M  F+PKADA   M+K +     D+  IEI+
Sbjct: 244 AGDMFKFIPKADA--IMMKCILHDWDDKECIEIL 264

BLAST of MELO3C013315 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.0e-37
Identity = 98/267 (36.70%), Postives = 154/267 (57.68%), Query Frame = 0

Query: 13  KEEEEEAKIQ----IYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQLSTALNCS 72
           + ++ EA ++    ++K + G A+  V+KCA+EL+I +++ SH  P +TL+Q+++++  S
Sbjct: 5   RNDQTEAALRGEANVWKSINGIADFMVMKCALELRIPDIVHSHSAP-ITLAQIASSVPDS 64

Query: 73  PPL----LFRIMRFLTHRGIFREETTPE-NLIAYSQTPLSHML---------INVAPLLL 132
           P L    L RIMR L  R IF +  + +   + Y  T  S +L         + +AP + 
Sbjct: 65  PSLNLSYLSRIMRLLVRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQVTLAPFVA 124

Query: 133 LENSPEIVASWHNLSAKLKNSYNNNENSDLVVPFEVAH-GKDIWSYGARNPGFNRMFNEG 192
               P + A W  L+  +K   N          FE+ H G+ +W     NP FN++FN+G
Sbjct: 125 FMTHPYLSAPWSCLARCVKEGGNG---------FEMVHGGRQLWDLSPGNPEFNKVFNDG 184

Query: 193 LGCNARVITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVS 252
           +   AR+ T+ AIL    D+F G+ SLVDVGG  G ++S +VK+ P +KGIN+DLP+VV+
Sbjct: 185 MASTARITTM-AILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVA 244

Query: 253 TSEKYDG-VEHVGGNMLDFVPKADAAF 260
           T+  Y G V HVGGNM +++P A A F
Sbjct: 245 TAPTYTGLVSHVGGNMFEWIPTAVAVF 260

BLAST of MELO3C013315 vs. ExPASy TrEMBL
Match: A0A5A7TFY9 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold460G00400 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 4.8e-160
Identity = 288/290 (99.31%), Postives = 288/290 (99.31%), Query Frame = 0

Query: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60
           MEETITKESKWK EEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ
Sbjct: 1   MEETITKESKWKNEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60

Query: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120
           LSTALNCSPP LFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE
Sbjct: 61  LSTALNCSPPHLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120

Query: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180
           IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV
Sbjct: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180

Query: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240
           ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG
Sbjct: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240

Query: 241 VEHVGGNMLDFVPKADAAFFMLKSLDQLVIEIVYITASGLLDADTLKSVK 291
           VEHVGGNMLDFVPKADAAFFMLKSLDQLVIEIVYITASGLLDADTLKSVK
Sbjct: 241 VEHVGGNMLDFVPKADAAFFMLKSLDQLVIEIVYITASGLLDADTLKSVK 290

BLAST of MELO3C013315 vs. ExPASy TrEMBL
Match: A0A1S4DWV8 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490399 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 9.4e-148
Identity = 270/287 (94.08%), Postives = 272/287 (94.77%), Query Frame = 0

Query: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60
           MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ
Sbjct: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60

Query: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120
           LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE
Sbjct: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120

Query: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180
           IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV
Sbjct: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180

Query: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240
           ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG
Sbjct: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240

Query: 241 VEHVGGNMLDFVPKADAAFFMLKSLDQLVIEIVYITASGLLDADTLK 288
           VEHVGGNMLDFVPKADAAFFM+      VI I  I A G+LD    K
Sbjct: 241 VEHVGGNMLDFVPKADAAFFMVSMFINFVILI--IIALGVLDGTYTK 285

BLAST of MELO3C013315 vs. ExPASy TrEMBL
Match: A0A5D3DI71 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004320 PE=3 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 3.0e-146
Identity = 261/261 (100.00%), Postives = 261/261 (100.00%), Query Frame = 0

Query: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60
           MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ
Sbjct: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60

Query: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120
           LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE
Sbjct: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120

Query: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180
           IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV
Sbjct: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180

Query: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240
           ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG
Sbjct: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240

Query: 241 VEHVGGNMLDFVPKADAAFFM 262
           VEHVGGNMLDFVPKADAAFFM
Sbjct: 241 VEHVGGNMLDFVPKADAAFFM 261

BLAST of MELO3C013315 vs. ExPASy TrEMBL
Match: A0A0A0K1F0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G017710 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 3.7e-136
Identity = 246/261 (94.25%), Postives = 253/261 (96.93%), Query Frame = 0

Query: 1   MEETITKESKWKKEEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60
           ME+TITKES WKK EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ
Sbjct: 1   MEQTITKESIWKK-EEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 60

Query: 61  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 120
           LST L CSPPLLFRIMRFLTHRGIF+EE TPENLIAYSQTPLSHMLINVAPLLLLENSPE
Sbjct: 61  LSTVLICSPPLLFRIMRFLTHRGIFKEEITPENLIAYSQTPLSHMLINVAPLLLLENSPE 120

Query: 121 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 180
           IVASW NLSA+L+NSYNNN++SDL+VPFEVAHGKDIWSYGARNPGFNRMFNEGL CNARV
Sbjct: 121 IVASWQNLSARLQNSYNNNDDSDLLVPFEVAHGKDIWSYGARNPGFNRMFNEGLACNARV 180

Query: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 240
           ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVV TSEKYDG
Sbjct: 181 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVLTSEKYDG 240

Query: 241 VEHVGGNMLDFVPKADAAFFM 262
           VEHVGGNMLDFVPKADAAFFM
Sbjct: 241 VEHVGGNMLDFVPKADAAFFM 260

BLAST of MELO3C013315 vs. ExPASy TrEMBL
Match: A0A6J1I1Q8 (xanthohumol 4'-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111468230 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 1.4e-103
Identity = 195/261 (74.71%), Postives = 221/261 (84.67%), Query Frame = 0

Query: 3   ETITKESKWKK--EEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTLSQ 62
           E  +KES WKK  EEEEEAKIQIYKY+FGFAEAAVIKCAIEL+IA+ IESHG P MTLSQ
Sbjct: 2   EITSKESSWKKEEEEEEEAKIQIYKYIFGFAEAAVIKCAIELQIADAIESHGSP-MTLSQ 61

Query: 63  LSTALNCSPPLLFRIMRFLTHRGIFREETTPENLIAYSQTPLSHMLINVAPLLLLENSPE 122
           LS+ALNCSP LLFRI+RFL HRGIF+++TT E+ IAYS TPLS ML  +APLLLLENSP 
Sbjct: 62  LSSALNCSPSLLFRILRFLVHRGIFKQQTTTESPIAYSHTPLSRMLTTMAPLLLLENSPT 121

Query: 123 IVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARV 182
           +VA WHNLSAK+K     + N      FE AHGKDIWSY A NP FN +FNEGLGCNARV
Sbjct: 122 MVAPWHNLSAKVKADAAGHSNL-----FEAAHGKDIWSYAAANPAFNTLFNEGLGCNARV 181

Query: 183 ITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDG 242
           +TLPAIL +C D+FNGV SLVDVGGGNGTTLS+LVKA PWMKGINFDLPNVV+TS++Y+G
Sbjct: 182 VTLPAILGSCRDMFNGVGSLVDVGGGNGTTLSLLVKACPWMKGINFDLPNVVNTSQEYEG 241

Query: 243 VEHVGGNMLDFVPKADAAFFM 262
           V+HVGG+M D+VPKADAAFFM
Sbjct: 242 VQHVGGSMFDYVPKADAAFFM 256

BLAST of MELO3C013315 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 223.8 bits (569), Expect = 1.8e-58
Identity = 116/255 (45.49%), Postives = 165/255 (64.71%), Query Frame = 0

Query: 14  EEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESH-GRPTMTLSQLSTALNCSPPLL 73
           +EE +A + I+KYVFGFA+ A  KCAI+LKI   IE+H     +TL++LS+A++ SP  L
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHL 83

Query: 74  FRIMRFLTHRGIFREETTPENL-IAYSQTPLSHMLI-------NVAPLLLLENSPEIVAS 133
            RIMRFL H+GIF+E  T + L   Y  TPLS  L+       ++AP +L E +PE++A 
Sbjct: 84  RRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAP 143

Query: 134 WHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARVITLP 193
           W  LS+ + +  N +       PF+  HGKD+WS+   NP  + M NE + C+AR + +P
Sbjct: 144 WLRLSSVVSSPVNGSTPP----PFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRV-VP 203

Query: 194 AILENCGDIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDGVEHV 253
            +   C  +F+GV ++VDVGGG G T+ +LVK FPW+KG NFDLP+V+  +E  DGVE+V
Sbjct: 204 RVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENV 263

Query: 254 GGNMLDFVPKADAAF 260
            G+M D +P  DA F
Sbjct: 264 EGDMFDSIPACDAIF 273

BLAST of MELO3C013315 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.3e-45
Identity = 98/246 (39.84%), Postives = 144/246 (58.54%), Query Frame = 0

Query: 14  EEEEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESH-GRPTMTLSQLSTALNCSPPLL 73
           +EE +A + I++YVFGFA+ A  KCAI+LKI   IE+H     +TLS+LS+A++ SP  L
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSHL 69

Query: 74  FRIMRFLTHRGIFREETTPENL-IAYSQTPLSHMLINVAPLLLLENSPEIVASWHNLSAK 133
            RIMRFL H+G+F+E  T + L   Y+ TPLS  +                     +  K
Sbjct: 70  RRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRM---------------------MITK 129

Query: 134 LKNSYNNNENSDLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARVITLPAILENCG 193
           L                   HGKD+W++   N   +++ NE + C+AR + +P +   C 
Sbjct: 130 L-------------------HGKDLWAFAQDNLCHSQLINEAMACDARRV-VPRVAGACQ 189

Query: 194 DIFNGVESLVDVGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDGVEHVGGNMLDF 253
            +F+GV ++VDVGGG G T+ +LVK FPW+KG NFDLP+V+  ++  DGVE+V G+M D 
Sbjct: 190 GLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDS 214

Query: 254 VPKADA 258
           +P +DA
Sbjct: 250 IPASDA 214

BLAST of MELO3C013315 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 128.3 bits (321), Expect = 1.0e-29
Identity = 82/237 (34.60%), Postives = 124/237 (52.32%), Query Frame = 0

Query: 36  IKCAIELKIANVIESHG---RPTMTLSQLSTALNCSPPLLFRIMRFLTHRGIF----REE 95
           +K A+EL +  ++  +G    PT   S+L T    +P +L RI+R LT   +     R+ 
Sbjct: 36  LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKL 95

Query: 96  TTPENLIAYSQTPLSHML------INVAPLLLLENSPEIVASWHNLSAKLKNSYNNNENS 155
           +       Y   P+   L      +++A L L+     ++ SW++L   +          
Sbjct: 96  SGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAI---------L 155

Query: 156 DLVVPFEVAHGKDIWSYGARNPGFNRMFNEGLGCNARVITLPAILENCGDIFNGVESLVD 215
           D  +PF  A+G   + Y   +P FN++FN G+  N   IT+  ILE     F G+ SLVD
Sbjct: 156 DGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMS-NHSTITMKKILETYKG-FEGLTSLVD 215

Query: 216 VGGGNGTTLSVLVKAFPWMKGINFDLPNVVSTSEKYDGVEHVGGNMLDFVPKADAAF 260
           VGGG G TL ++V  +P +KGINFDLP+V+  +  + G+EHVGG+M   VPK DA F
Sbjct: 216 VGGGIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIF 261

BLAST of MELO3C013315 vs. TAIR 10
Match: AT5G53810.1 (O-methyltransferase family protein )

HSP 1 Score: 105.5 bits (262), Expect = 7.2e-23
Identity = 83/284 (29.23%), Postives = 134/284 (47.18%), Query Frame = 0

Query: 1   MEETITKESKWKKEE--EEEAKIQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPTMTL 60
           ++  +TK  + K+E+  EEEA++   +     A   V+K A+EL + + I + G   + L
Sbjct: 5   LQVPLTKPDRVKEEQEVEEEARLLARRLANAAASPMVLKAALELGVIDTITTVGGGDLWL 64

Query: 61  SQLSTALNC--------SPPLLFRIMRFLTHRGIFREETTPEN-------LIAYSQTPLS 120
           S    AL          +P LL R++RFL    + +  T  E           Y+  P+ 
Sbjct: 65  SPSEIALRLPTKPCNLEAPALLDRMLRFLVSHSVLKCRTVIEENGQTGKVERVYAAEPVC 124

Query: 121 HMLIN--------VAPLLLLENSPEIVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKD 180
             L+N         A L +L+ S   + +W +L   +       E  D    F  AHG  
Sbjct: 125 KYLLNKSDDVSGSFASLFMLDLSDVFIKTWTHLEDVIL------EGRD---AFSSAHGMK 184

Query: 181 IWSYGARNPGFNRMFNEGLGCNARVITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLV 240
           ++ Y   +  F ++FN  +  ++ ++T   +    G  F  V++LVDVGGG G TL ++ 
Sbjct: 185 LFEYIQADERFGKVFNRAMLESSTMVTEKVLKFYEG--FKDVKTLVDVGGGLGNTLGLIT 244

Query: 241 KAFPWMKGINFDLPNVVSTSEKYDGVEHVGGNMLDFVPKADAAF 260
             +P + GINFDL  V++ +  Y GV HV G+M   +PK DA F
Sbjct: 245 SKYPHLIGINFDLAPVLANAHSYPGVNHVAGDMFIKIPKGDAIF 277

BLAST of MELO3C013315 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 95.1 bits (235), Expect = 9.7e-20
Identity = 80/281 (28.47%), Postives = 135/281 (48.04%), Query Frame = 0

Query: 4   TITKESKWKKEEEEEAK---IQIYKYVFGFAEAAVIKCAIELKIANVIESHGRPT-MTLS 63
           T  K    K+E+E + K   +Q    V   A   V+K A+EL + + I +    T ++ S
Sbjct: 11  TYPKPGLTKEEQEIDEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPS 70

Query: 64  QLSTALNC------SPPLLFRIMRFLTHRGIFR--------EETTPENLIAYSQTPLSHM 123
           +++ +L        +P LL R++R L    I +           T +    Y+  P+   
Sbjct: 71  EIAVSLPNKPTNPEAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKY 130

Query: 124 LI-------NVAPLLLLENSPEIVASWHNLSAKLKNSYNNNENSDLVVPFEVAHGKDIWS 183
            +       +++ LLLL +S  I+ +W NL   +       E  D    F  AH   ++ 
Sbjct: 131 FLKDSDGSGSLSSLLLLLHSQVILKTWTNLKDVIL------EGKD---AFSSAHDMRLFE 190

Query: 184 YGARNPGFNRMFNEGLGCNARVITLPAILENCGDIFNGVESLVDVGGGNGTTLSVLVKAF 243
           Y + +  F+++F+  +  ++ ++    + E  G  F  V +LVDVGGG GT L ++   +
Sbjct: 191 YISSDDQFSKLFHRAMSESSTMVMKKVLEEYRG--FEDVNTLVDVGGGIGTILGLITSKY 250

Query: 244 PWMKGINFDLPNVVSTSEKYDGVEHVGGNMLDFVPKADAAF 260
           P +KG+NFDL  V++ +  Y GV+HV G+M   VPK DA F
Sbjct: 251 PHIKGVNFDLAQVLTQAPFYPGVKHVSGDMFIEVPKGDAIF 280