MELO3C013178 (gene) Melon (DHL92) v4

Overview
NameMELO3C013178
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
Description1-phosphatidylinositol-3-phosphate 5-kinase
Locationchr01: 13087988 .. 13094470 (-)
RNA-Seq ExpressionMELO3C013178
SyntenyMELO3C013178
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTATTCAAGAAGCATGCAGCTCACAAACACATGCCGACACATTGTAAGAACCCGAAGTTAGTACTGATTCAAGGCATGCTTGGTGAGGCTCCAATAAGTAGACTGTCTTCATTTAATTCAATGGATCAGGTACTGAAATTTAGACGTTTCTGTCTGACTGAGAAATACACCTGTATGTCTTCTGATTTCTTTTTTGTCTGATCACAGGAAAATGATTTTACGAATCATGTTATAGAGATGATTGAAGGTTGCACTGCAAATGTTATTTTAGTTGAAAAAACTGCAGCACGAGTTATCCAAGAAGCAATTCTCAAGAAAGGCATGACATTAGTGCTTGATATGAAGCTCCACCGTCTTCAGAGAATAGCTCTTTGTACTGGTTCACCAATTTTAACATCTGAAACTTTAATGAGCCAAAAGACAAGACAGTGTGATGCTGTTTATTTTCAAAAAATTGTAGAAGAACATGCTGGTGTTTTGGAGGGAGGAAAGATGTCAACTAAAACTTTAATGTTTATTGAGGGCTGTCCAACACGTTTGGGTTGTACGGTTAGTGTGCTATTTCTAGTTGGCTCTCTCTACCATTATATATTGAGTTTGAGATAGATTCATTCATTACCAATCAACTACGCAAATGGATGGTTGGTGTTCTCTGGCTGTTTAATTGCTGGTTTCATTTTTGTGAAGGAAAAAGAAAAGATTTTGTGTTACTGTAGACAATTCCTTGAGTGTGAAAATAAGTTAAGTTAAAAGAACTTTAGCTTTTTCAAAGCTCCATCCTGGCAAGAAATACTCCTTTTTTTAGGGGCACAAACATTAGTTGAGACACAAGCATTAGTTGGGACACATGTTTCAGTTTTGTAAGAGTTTGAGCCATATCATGCAATTTTGTCTTTTGACAGCCTTCTAAAACAAAGATGAGAATATTGGTTGCATGAATTCTTGTAGGATTACACTAGCATGGTCTTTGATTTAATCCCTATATCTGCAGAGAGTTGCACTCACTTTTTATGTTCTAATTTTTCATCTCACCATCATATGGGGTGTGTGGTGTTGCCTCTTTCTAAATTTTTTTTGTATTATGACATCCATGGTTGGACTTTGATATTGTTTTGCCTGATGATAGATTAAATTCCAAATTAGAATATTGATTTAATTGACCGTCTTTTGGGCACAAATGAACTATGTACTTAAAATCTGCTGCAGATTTTGCTGAAAGGAGCTCACAGTGATGAACTGAAGAGGGTTAAGTTGGTTGTGCAGTGTGCCGTTGTTATGGCATTTCAATTAATATTAGAGACTTCTTTCATTGTTGACCAGCAAGCTATGTTTGCAACTATTCCATTTGGTGGAGTACCATCGATTGCTTCATCTGATCCCCAATCACCTTTAGAGCCTCGTAGCCCAAAAGTGGGTCAAGCTGAGAGTGCAAGTGATCAAATTGAACAACCAACGAATATTCATATTTCAAGTGGATCCCATGAGGAGGCATCCAACGAATCAAACGTGGAGACGGTGGAAAAGCTAATAGTGTCCTCTGAACCAGAACCATATAATCCTGCCATATTTTCTGGATTCTCATCCATTTCTGATTCATTAAAGAGAGCTATGGGGGAAAGTTTCCTTTTAACCTCTCCCTATCTGTCATTGTCTTCTTATTTTGGACATGAGAGTGACTTGAGTGGCTTGGTTGCAAAGTCTGACTCAATTCCATCTACTCTACAGGCAGTCTATCAGTTTGATGAGGAAGTTAGGGGCAGTTCTGATGGAGAGAATTCCGTTCATGAACAGTCTGTGTCACATCAATCAACCTTGGAAGGCCTTGGATTCCATGAAACTGCTCCTAACGATACCGGAGACACAATGCAGAAGAAACCGCCATTAGATTCCCAGAGTATTTTGGTTTTGATGTCGAGCCGAAATGCATTGAAGGGAACCATGTGTGAGCAGAGCCATTTTTCACATATTGTGTTCTACAAGAATTTTGATGTTCCTCTAGGGAAATTTTTACAAGAAAATTTACTAAATCAGGTTTGAAATGGAGCCATGATTACCTACTACATTTTAATATACAATTTACTAAATTCACTCGTTAATTTATCTTATAGCATGTGGAATACTAGTTTATAATAATCAATAATTTTGTATATATATATTTTGAAGTTTATATACTTCTTAGCTTCTGGTAAAGCTGCAATAAGACTAGAAGCTGCAGTAAGACTATGATGCATCTAATTATCTTGACTCGTTTGTTTTACCAATGAGAAAAACCATCTGTGCATGAGGCTACTGAGTTTGGAATAGCCCTAATCATAAACTATAAGAGTCAACATTTTCTAAGATGGTTCTGAACTTTGGTGCAACTGCAGAATAATCTCTGCACTGTCTGTGGCGAACTGCCAGAAGCTCACTTTTACTATTATGCACACTATCGAAAGCAGCTTTCCATTCAAGTCAAACAGCTTCCAGGAGATAAAGTTTTGCCAGGTGAAACAGAAGGAAAACTTTGGATGTGGAGTCGTTGCAGTAAGTGTAAATCTCTGGGAGGACCTTCAAAGTCCACAAAAAGGGTGCTGATCTCTACAGCTGCACGTGGCTTATCATTTGGGAAGTTTTTAGAGCTTTGTTTCTCTGATGACACTTTACCTAGTAAATCATCAGTTTGTGGTCATTCTTTGTTTGGGGACTTCCTCTACTTCTTCGGGTACGTGTTGCCATAGACTGGATATTTACTTGTGCTTGCATTTTCATTTTTTCTGTCCTGAACTCAGTCTTCCTTGATTATTATCATTGTGGTTTGTAGACTGGGCAATATGGTTGCTATGTTCAGATATTCTGAGGTAGCAATTTATACGGTCTCTATGCCTCCTCAGAAATTGGAGTTCAACAGTTCAATGAGACAAGGTCATCTCATCAAAGAAATTGAAAATGTATGTAATCTTATCTATGTTTGTCTGTCACCCTTCTCTGCTTTATGATGAGATTCCAATTGATTTCTTCTAATAGTGTATGGATTACTCAATTGTGAAGTGTGTGATTTTTTAACATCTGGTCAAGGTTTACACGAAAGGGATGTTACTTTTCTCTGAGATTGCACACTCTCTGAAGAAGATCATATCTGAACGTAATAGTTCAAATGTAAACCACCCTCAAGGCTTTCTCAATGACTTTTCTTTTGTTGAAGAAATGTTAAATGAAGAACGATCAGAATTTGAGGTTAGCTTTTTGTTGTTGTCTCCTTGGAATTCTCTGGAACTTTTACTCCTTTTGAATTCCTTCTCCTTTTCGTTTATGATGAAGTGCATCCTCTTATTTCTATGCATTAAGGCATACTTTTCCTTTTTTTTTTAATTGAATGAAATGCAGGTAAAAATTCAAAATTCTCTTACTAAGAAAGGAAACCTGGACTTGGCTTTTCACAAATTTCTCAATTTGAATCGATTGCTCTGGGACCTGTTGCTAGAATCATGTATTTGGGATAGACGCCTGCAATCATTAGCATCTTTGGGCGTTACAACAAACTCTGGCACCTCTGAAAATGTTGAGCCAGAACCAGTTATGTTGAAGATGAATAGCAACATCAATGTTGGACCTGAGGAATCAGAATCAATTGCAGAAAATGATGATACAAAAATTCAGCAAGATATATCCGTAGATGAAAATGTATTGCCTCTGAAAGAAATTGCAGTTGAAGGTTCGGATGGAGAGTCGGGTGGTGATGAACTTAATTTACCTTCAGCGATTGAAGTCACGGAGATACAGATTATAGATGATTTGAGCCCCAAAAAACTTTCTCGCCAAGGGACTTTGTCAAATGGCTTTGACTGTCATCATTCGGATTATGAGGATTCTCAAGTAGGAAGAGTTCTGTCATCTGGTGATTTGCATGTAGATAGGACAATTCCAATTTCAATAGGTGATTCGACCCTCAGCAAATTGTTTTGTACACCGTTTTCGGAGATAAGGCAGATGCACTTGAGGGACATCCAGAGAAGTTACTTTCCCGAACTCAAATCCATCAGTAGCTATACTCCAAAACTCTTAACCGCAGCCTCTGACTTCATCCATGAAGAAGGACAGAAGCTGCACATTCATCTTGCTGATGATAATTTTGTTGTTTCAGACTATGAGGGTGAACTCTCGAGCATAGTTGCTTGTGTACTTGCTTTGTTGAAAGATCAACCCTTTCAAACAGATTCTCATAATGAGGATAGCAAGGGAGAGGGAGGAGGCGCTTTACAACCAGCTTTAAGTTCACGAAGCCTAAATAAAGTTCCCTCAAATGGATCCTCAGATTCAGAGTCTAGCATCTCTTCAGAAGAATATCGGTTCTCAAGCTTTGACAGGTTAAATTTGTTGGATTCTCTTGTTTCAGAAACTTTCAAGCGATCGGATCATGAAGGGGTCATAAAGTCACTAGCAAAGGGAAAATATCTTGTGAATTGCCCATATATCAATCAGTTTCGTGATCTTCGGAGTCGATGCTGCCCGTCTGAGCTCCATTTCATAGCTTCTTTGAGTCGTTGTATAAACTGGAACGCAAAAGGTGGGAAGAGCAAATCTTTTTTTGCCAAGACACTTGATGACAGGTTCATTATCAAAGAAATTAAAAGAACTGAGTATGATTCATTTATGAAGTTTGCTCCAGAGTATTTCAAGTACATTAATGAATCATTTGACATGGGGAACCAGACATGCCTTGCTAAAGTTCTTGGAATCTATCAGGTTTGATAGACATAACATTCATCTCTTTGATATTTTATACCACCTCCACCCTTGGTTGTCCTTATTAACATTCTTTTTTAGGTAACGGTGAGAGAGCAAAAGAGTGGGAAAGAGATGAGACATGATTTGATGGTTATGGAGAACCTCAGTTTCGGTCGCAACATAATTCGCCAATATGATCTTAAAGGAGCTCTACATGCTCGATTTAATCCAGCTACCAACAATTTTGGTGAAGTCCTCTTAGATCAGAACTTTGTGAATGACATGAACTCATCTCCCCTCTATGTGAGTAATCGAGCAAAGCGTCGGTTGCAACGGGCGATTTGGAATGATACCACTTTTCTCAATGTGAGTACAAAACTGATTCTTTCACTCATTGAAGTCCTCTCCCCCTGATTGATGAGATCTTTTTGTTTAAAGGTTTATCAGATTTGATATAGTTGTGTTTCGTTGTTGCTTATCATTATTTTTTATTTGTGCTTAGGATGACTTGTTACTGACCTGGTTTTGTATTTTGTATATTTGTATCATCTTTCAATAATAAAAAAATTAAGTTTTACTAGTTTAATTAGCTACAGACTACAGTTTAAGCTTTAAGGACTACTGGTAGGTGGGGTGTGAAGAGTTAAAAGGAAGTGTCACAATCATAACAGATCTTGTTTGGACATTTTACAAGCCTCATCTTAAGTTGTTTGGTTCACTTGCAATAATAGCTTATCTTGCATAGTTTGGTTTTGGAAGATTTCTGAGCATTTCAAGAATAGTCTCTTCTTTGGTTTATGAGTTTTCTCTCTTGTTTTATTTGGATTATTTTAGCTCTCTGCTTAGATCTTACATTTTGTTAGTTTGAAGGTTTGATTATGGAGTCTGCAGGTAGATGCTTTAGTTTTTTCAGATCTTGTTGTATTTCTCCTACCTTCCCTCATTTAGACTTGTGGATCTGATTGAACCCCAAGAATGCATTAGGAAGGTTAATTTTTCTGACATTTTTTGCATAATGCTTGCAGTCGATAAATGTTATGGATTATTCTCTGCTTGTTGGGGTTGATGCCGAAAAGAAGGAACTTGTATGTGGGATAATCGATTACCTTAGACAATACACGTGGGACAAGCAACTAGAGACGTGGGTGAAATCATCCCTCATACCAAAAAATGTACTTCCGACCGTTGTTTCTCCAAAAGAGTACAAGAGGAGATTTAGAAAGTTCATGTCAGCACATTTCTTGAGTGTCCCAGATCATTGGTGCATGCAGCAGAACCTCCCTGGGCCTTGGGACCTCTGTGGCACCGCAGATTGTGCTTCTTCTAATAAGACGAATGCCAATGAAAATGTTCTCTCTTCATAAAGTCCAAAGGAGCAGCTCGGTTTTTTGTAATCAAAATGGAGAAAATGTGGATTGATGAAGGAAGGTGTTCTCTCTTCATGACCTCTTTTGACTAAAGTCCAGCTGCCTCATTATGCTTGCAAGTTCATTCTATGGTTGTAAATAGATGGCTGACATGCCATTCAGTTTTATCATGAGTTTGCCCTGATATTCTGCAAATTTTTTGTTAGCAAAAAGTCTAGGGTGGTGAAATTACTGGTAGGTTGCCTCCGATCATGCTCAGGTTGCCTAGTTGAGGGGCAAAAGGGTAATTGTGTAGTTAAAAGTGTATAGGTTATATAGTAGCTGAAGATTTGTTAATTCTTGTGAAAATATATAAAATCTTCATTCATCTGAGATGTATTAATTGATGATGTTCATTCATTTTCAC

mRNA sequence

ATGGTATTCAAGAAGCATGCAGCTCACAAACACATGCCGACACATTGTAAGAACCCGAAGTTAGTACTGATTCAAGGCATGCTTGGTGAGGCTCCAATAAGTAGACTGTCTTCATTTAATTCAATGGATCAGGAAAATGATTTTACGAATCATGTTATAGAGATGATTGAAGGTTGCACTGCAAATGTTATTTTAGTTGAAAAAACTGCAGCACGAGTTATCCAAGAAGCAATTCTCAAGAAAGGCATGACATTAGTGCTTGATATGAAGCTCCACCGTCTTCAGAGAATAGCTCTTTGTACTGGTTCACCAATTTTAACATCTGAAACTTTAATGAGCCAAAAGACAAGACAGTGTGATGCTGTTTATTTTCAAAAAATTGTAGAAGAACATGCTGGTGTTTTGGAGGGAGGAAAGATGTCAACTAAAACTTTAATGTTTATTGAGGGCTGTCCAACACGTTTGGGTTGTACGATTTTGCTGAAAGGAGCTCACAGTGATGAACTGAAGAGGGTTAAGTTGGTTGTGCAGTGTGCCGTTGTTATGGCATTTCAATTAATATTAGAGACTTCTTTCATTGTTGACCAGCAAGCTATGTTTGCAACTATTCCATTTGGTGGAGTACCATCGATTGCTTCATCTGATCCCCAATCACCTTTAGAGCCTCGTAGCCCAAAAGTGGGTCAAGCTGAGAGTGCAAGTGATCAAATTGAACAACCAACGAATATTCATATTTCAAGTGGATCCCATGAGGAGGCATCCAACGAATCAAACGTGGAGACGGTGGAAAAGCTAATAGTGTCCTCTGAACCAGAACCATATAATCCTGCCATATTTTCTGGATTCTCATCCATTTCTGATTCATTAAAGAGAGCTATGGGGGAAAGTTTCCTTTTAACCTCTCCCTATCTGTCATTGTCTTCTTATTTTGGACATGAGAGTGACTTGAGTGGCTTGGTTGCAAAGTCTGACTCAATTCCATCTACTCTACAGGCAGTCTATCAGTTTGATGAGGAAGTTAGGGGCAGTTCTGATGGAGAGAATTCCGTTCATGAACAGTCTGTGTCACATCAATCAACCTTGGAAGGCCTTGGATTCCATGAAACTGCTCCTAACGATACCGGAGACACAATGCAGAAGAAACCGCCATTAGATTCCCAGAGTATTTTGGTTTTGATGTCGAGCCGAAATGCATTGAAGGGAACCATGTGTGAGCAGAGCCATTTTTCACATATTGTGTTCTACAAGAATTTTGATGTTCCTCTAGGGAAATTTTTACAAGAAAATTTACTAAATCAGAATAATCTCTGCACTGTCTGTGGCGAACTGCCAGAAGCTCACTTTTACTATTATGCACACTATCGAAAGCAGCTTTCCATTCAAGTCAAACAGCTTCCAGGAGATAAAGTTTTGCCAGGTGAAACAGAAGGAAAACTTTGGATGTGGAGTCGTTGCAGTAAGTGTAAATCTCTGGGAGGACCTTCAAAGTCCACAAAAAGGGTGCTGATCTCTACAGCTGCACGTGGCTTATCATTTGGGAAGTTTTTAGAGCTTTGTTTCTCTGATGACACTTTACCTAGTAAATCATCAGTTTGTGGTCATTCTTTGTTTGGGGACTTCCTCTACTTCTTCGGACTGGGCAATATGGTTGCTATGTTCAGATATTCTGAGGTAGCAATTTATACGGTCTCTATGCCTCCTCAGAAATTGGAGTTCAACAGTTCAATGAGACAAGGTCATCTCATCAAAGAAATTGAAAATGTTTACACGAAAGGGATGTTACTTTTCTCTGAGATTGCACACTCTCTGAAGAAGATCATATCTGAACGTAATAGTTCAAATGTAAACCACCCTCAAGGCTTTCTCAATGACTTTTCTTTTGTTGAAGAAATGTTAAATGAAGAACGATCAGAATTTGAGGTAAAAATTCAAAATTCTCTTACTAAGAAAGGAAACCTGGACTTGGCTTTTCACAAATTTCTCAATTTGAATCGATTGCTCTGGGACCTGTTGCTAGAATCATGTATTTGGGATAGACGCCTGCAATCATTAGCATCTTTGGGCGTTACAACAAACTCTGGCACCTCTGAAAATGTTGAGCCAGAACCAGTTATGTTGAAGATGAATAGCAACATCAATGTTGGACCTGAGGAATCAGAATCAATTGCAGAAAATGATGATACAAAAATTCAGCAAGATATATCCGTAGATGAAAATGTATTGCCTCTGAAAGAAATTGCAGTTGAAGGTTCGGATGGAGAGTCGGGTGGTGATGAACTTAATTTACCTTCAGCGATTGAAGTCACGGAGATACAGATTATAGATGATTTGAGCCCCAAAAAACTTTCTCGCCAAGGGACTTTGTCAAATGGCTTTGACTGTCATCATTCGGATTATGAGGATTCTCAAGTAGGAAGAGTTCTGTCATCTGGTGATTTGCATGTAGATAGGACAATTCCAATTTCAATAGGTGATTCGACCCTCAGCAAATTGTTTTGTACACCGTTTTCGGAGATAAGGCAGATGCACTTGAGGGACATCCAGAGAAGTTACTTTCCCGAACTCAAATCCATCAGTAGCTATACTCCAAAACTCTTAACCGCAGCCTCTGACTTCATCCATGAAGAAGGACAGAAGCTGCACATTCATCTTGCTGATGATAATTTTGTTGTTTCAGACTATGAGGGTGAACTCTCGAGCATAGTTGCTTGTGTACTTGCTTTGTTGAAAGATCAACCCTTTCAAACAGATTCTCATAATGAGGATAGCAAGGGAGAGGGAGGAGGCGCTTTACAACCAGCTTTAAGTTCACGAAGCCTAAATAAAGTTCCCTCAAATGGATCCTCAGATTCAGAGTCTAGCATCTCTTCAGAAGAATATCGGTTCTCAAGCTTTGACAGGTTAAATTTGTTGGATTCTCTTGTTTCAGAAACTTTCAAGCGATCGGATCATGAAGGGGTCATAAAGTCACTAGCAAAGGGAAAATATCTTGTGAATTGCCCATATATCAATCAGTTTCGTGATCTTCGGAGTCGATGCTGCCCGTCTGAGCTCCATTTCATAGCTTCTTTGAGTCGTTGTATAAACTGGAACGCAAAAGGTGGGAAGAGCAAATCTTTTTTTGCCAAGACACTTGATGACAGGTTCATTATCAAAGAAATTAAAAGAACTGAGTATGATTCATTTATGAAGTTTGCTCCAGAGTATTTCAAGTACATTAATGAATCATTTGACATGGGGAACCAGACATGCCTTGCTAAAGTTCTTGGAATCTATCAGGTAACGGTGAGAGAGCAAAAGAGTGGGAAAGAGATGAGACATGATTTGATGGTTATGGAGAACCTCAGTTTCGGTCGCAACATAATTCGCCAATATGATCTTAAAGGAGCTCTACATGCTCGATTTAATCCAGCTACCAACAATTTTGGTGAAGTCCTCTTAGATCAGAACTTTGTGAATGACATGAACTCATCTCCCCTCTATGTGAGTAATCGAGCAAAGCGTCGGTTGCAACGGGCGATTTGGAATGATACCACTTTTCTCAATTCGATAAATGTTATGGATTATTCTCTGCTTGTTGGGGTTGATGCCGAAAAGAAGGAACTTGTATGTGGGATAATCGATTACCTTAGACAATACACGTGGGACAAGCAACTAGAGACGTGGGTGAAATCATCCCTCATACCAAAAAATGTACTTCCGACCGTTGTTTCTCCAAAAGAGTACAAGAGGAGATTTAGAAAGTTCATGTCAGCACATTTCTTGAGTGTCCCAGATCATTGGTGCATGCAGCAGAACCTCCCTGGGCCTTGGGACCTCTGTGGCACCGCAGATTGTGCTTCTTCTAATAAGACGAATGCCAATGAAAATGTTCTCTCTTCATAAAGTCCAAAGGAGCAGCTCGGTTTTTTGTAATCAAAATGGAGAAAATGTGGATTGATGAAGGAAGGTGTTCTCTCTTCATGACCTCTTTTGACTAAAGTCCAGCTGCCTCATTATGCTTGCAAGTTCATTCTATGGTTGTAAATAGATGGCTGACATGCCATTCAGTTTTATCATGAGTTTGCCCTGATATTCTGCAAATTTTTTGTTAGCAAAAAGTCTAGGGTGGTGAAATTACTGGTAGGTTGCCTCCGATCATGCTCAGGTTGCCTAGTTGAGGGGCAAAAGGGTAATTGTGTAGTTAAAAGTGTATAGGTTATATAGTAGCTGAAGATTTGTTAATTCTTGTGAAAATATATAAAATCTTCATTCATCTGAGATGTATTAATTGATGATGTTCATTCATTTTCAC

Coding sequence (CDS)

ATGGTATTCAAGAAGCATGCAGCTCACAAACACATGCCGACACATTGTAAGAACCCGAAGTTAGTACTGATTCAAGGCATGCTTGGTGAGGCTCCAATAAGTAGACTGTCTTCATTTAATTCAATGGATCAGGAAAATGATTTTACGAATCATGTTATAGAGATGATTGAAGGTTGCACTGCAAATGTTATTTTAGTTGAAAAAACTGCAGCACGAGTTATCCAAGAAGCAATTCTCAAGAAAGGCATGACATTAGTGCTTGATATGAAGCTCCACCGTCTTCAGAGAATAGCTCTTTGTACTGGTTCACCAATTTTAACATCTGAAACTTTAATGAGCCAAAAGACAAGACAGTGTGATGCTGTTTATTTTCAAAAAATTGTAGAAGAACATGCTGGTGTTTTGGAGGGAGGAAAGATGTCAACTAAAACTTTAATGTTTATTGAGGGCTGTCCAACACGTTTGGGTTGTACGATTTTGCTGAAAGGAGCTCACAGTGATGAACTGAAGAGGGTTAAGTTGGTTGTGCAGTGTGCCGTTGTTATGGCATTTCAATTAATATTAGAGACTTCTTTCATTGTTGACCAGCAAGCTATGTTTGCAACTATTCCATTTGGTGGAGTACCATCGATTGCTTCATCTGATCCCCAATCACCTTTAGAGCCTCGTAGCCCAAAAGTGGGTCAAGCTGAGAGTGCAAGTGATCAAATTGAACAACCAACGAATATTCATATTTCAAGTGGATCCCATGAGGAGGCATCCAACGAATCAAACGTGGAGACGGTGGAAAAGCTAATAGTGTCCTCTGAACCAGAACCATATAATCCTGCCATATTTTCTGGATTCTCATCCATTTCTGATTCATTAAAGAGAGCTATGGGGGAAAGTTTCCTTTTAACCTCTCCCTATCTGTCATTGTCTTCTTATTTTGGACATGAGAGTGACTTGAGTGGCTTGGTTGCAAAGTCTGACTCAATTCCATCTACTCTACAGGCAGTCTATCAGTTTGATGAGGAAGTTAGGGGCAGTTCTGATGGAGAGAATTCCGTTCATGAACAGTCTGTGTCACATCAATCAACCTTGGAAGGCCTTGGATTCCATGAAACTGCTCCTAACGATACCGGAGACACAATGCAGAAGAAACCGCCATTAGATTCCCAGAGTATTTTGGTTTTGATGTCGAGCCGAAATGCATTGAAGGGAACCATGTGTGAGCAGAGCCATTTTTCACATATTGTGTTCTACAAGAATTTTGATGTTCCTCTAGGGAAATTTTTACAAGAAAATTTACTAAATCAGAATAATCTCTGCACTGTCTGTGGCGAACTGCCAGAAGCTCACTTTTACTATTATGCACACTATCGAAAGCAGCTTTCCATTCAAGTCAAACAGCTTCCAGGAGATAAAGTTTTGCCAGGTGAAACAGAAGGAAAACTTTGGATGTGGAGTCGTTGCAGTAAGTGTAAATCTCTGGGAGGACCTTCAAAGTCCACAAAAAGGGTGCTGATCTCTACAGCTGCACGTGGCTTATCATTTGGGAAGTTTTTAGAGCTTTGTTTCTCTGATGACACTTTACCTAGTAAATCATCAGTTTGTGGTCATTCTTTGTTTGGGGACTTCCTCTACTTCTTCGGACTGGGCAATATGGTTGCTATGTTCAGATATTCTGAGGTAGCAATTTATACGGTCTCTATGCCTCCTCAGAAATTGGAGTTCAACAGTTCAATGAGACAAGGTCATCTCATCAAAGAAATTGAAAATGTTTACACGAAAGGGATGTTACTTTTCTCTGAGATTGCACACTCTCTGAAGAAGATCATATCTGAACGTAATAGTTCAAATGTAAACCACCCTCAAGGCTTTCTCAATGACTTTTCTTTTGTTGAAGAAATGTTAAATGAAGAACGATCAGAATTTGAGGTAAAAATTCAAAATTCTCTTACTAAGAAAGGAAACCTGGACTTGGCTTTTCACAAATTTCTCAATTTGAATCGATTGCTCTGGGACCTGTTGCTAGAATCATGTATTTGGGATAGACGCCTGCAATCATTAGCATCTTTGGGCGTTACAACAAACTCTGGCACCTCTGAAAATGTTGAGCCAGAACCAGTTATGTTGAAGATGAATAGCAACATCAATGTTGGACCTGAGGAATCAGAATCAATTGCAGAAAATGATGATACAAAAATTCAGCAAGATATATCCGTAGATGAAAATGTATTGCCTCTGAAAGAAATTGCAGTTGAAGGTTCGGATGGAGAGTCGGGTGGTGATGAACTTAATTTACCTTCAGCGATTGAAGTCACGGAGATACAGATTATAGATGATTTGAGCCCCAAAAAACTTTCTCGCCAAGGGACTTTGTCAAATGGCTTTGACTGTCATCATTCGGATTATGAGGATTCTCAAGTAGGAAGAGTTCTGTCATCTGGTGATTTGCATGTAGATAGGACAATTCCAATTTCAATAGGTGATTCGACCCTCAGCAAATTGTTTTGTACACCGTTTTCGGAGATAAGGCAGATGCACTTGAGGGACATCCAGAGAAGTTACTTTCCCGAACTCAAATCCATCAGTAGCTATACTCCAAAACTCTTAACCGCAGCCTCTGACTTCATCCATGAAGAAGGACAGAAGCTGCACATTCATCTTGCTGATGATAATTTTGTTGTTTCAGACTATGAGGGTGAACTCTCGAGCATAGTTGCTTGTGTACTTGCTTTGTTGAAAGATCAACCCTTTCAAACAGATTCTCATAATGAGGATAGCAAGGGAGAGGGAGGAGGCGCTTTACAACCAGCTTTAAGTTCACGAAGCCTAAATAAAGTTCCCTCAAATGGATCCTCAGATTCAGAGTCTAGCATCTCTTCAGAAGAATATCGGTTCTCAAGCTTTGACAGGTTAAATTTGTTGGATTCTCTTGTTTCAGAAACTTTCAAGCGATCGGATCATGAAGGGGTCATAAAGTCACTAGCAAAGGGAAAATATCTTGTGAATTGCCCATATATCAATCAGTTTCGTGATCTTCGGAGTCGATGCTGCCCGTCTGAGCTCCATTTCATAGCTTCTTTGAGTCGTTGTATAAACTGGAACGCAAAAGGTGGGAAGAGCAAATCTTTTTTTGCCAAGACACTTGATGACAGGTTCATTATCAAAGAAATTAAAAGAACTGAGTATGATTCATTTATGAAGTTTGCTCCAGAGTATTTCAAGTACATTAATGAATCATTTGACATGGGGAACCAGACATGCCTTGCTAAAGTTCTTGGAATCTATCAGGTAACGGTGAGAGAGCAAAAGAGTGGGAAAGAGATGAGACATGATTTGATGGTTATGGAGAACCTCAGTTTCGGTCGCAACATAATTCGCCAATATGATCTTAAAGGAGCTCTACATGCTCGATTTAATCCAGCTACCAACAATTTTGGTGAAGTCCTCTTAGATCAGAACTTTGTGAATGACATGAACTCATCTCCCCTCTATGTGAGTAATCGAGCAAAGCGTCGGTTGCAACGGGCGATTTGGAATGATACCACTTTTCTCAATTCGATAAATGTTATGGATTATTCTCTGCTTGTTGGGGTTGATGCCGAAAAGAAGGAACTTGTATGTGGGATAATCGATTACCTTAGACAATACACGTGGGACAAGCAACTAGAGACGTGGGTGAAATCATCCCTCATACCAAAAAATGTACTTCCGACCGTTGTTTCTCCAAAAGAGTACAAGAGGAGATTTAGAAAGTTCATGTCAGCACATTTCTTGAGTGTCCCAGATCATTGGTGCATGCAGCAGAACCTCCCTGGGCCTTGGGACCTCTGTGGCACCGCAGATTGTGCTTCTTCTAATAAGACGAATGCCAATGAAAATGTTCTCTCTTCATAA

Protein sequence

MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQPTNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGESFLLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVSHQSTLEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSEIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEESESIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLSPKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSEEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDHWCMQQNLPGPWDLCGTADCASSNKTNANENVLSS
Homology
BLAST of MELO3C013178 vs. NCBI nr
Match: TYK23139.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucumis melo var. makuwa])

HSP 1 Score: 2559.3 bits (6632), Expect = 0.0e+00
Identity = 1293/1296 (99.77%), Postives = 1294/1296 (99.85%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 60
            MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT
Sbjct: 308  MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 367

Query: 61   ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 120
            ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD
Sbjct: 368  ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 427

Query: 121  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 180
            AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV
Sbjct: 428  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 487

Query: 181  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP 240
            VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP
Sbjct: 488  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP 547

Query: 241  TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGESFLLT 300
            TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGESFLLT
Sbjct: 548  TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGESFLLT 607

Query: 301  SPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVSHQST 360
            SPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVSHQST
Sbjct: 608  SPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVSHQST 667

Query: 361  LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV 420
            LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV
Sbjct: 668  LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV 727

Query: 421  PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW 480
            PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW
Sbjct: 728  PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW 787

Query: 481  MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF 540
            MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF
Sbjct: 788  MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF 847

Query: 541  LYFFG--LGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSE 600
            LYFFG  LGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSE
Sbjct: 848  LYFFGYVLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSE 907

Query: 601  IAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFH 660
            IAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFH
Sbjct: 908  IAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFH 967

Query: 661  KFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEE 720
            KFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEE
Sbjct: 968  KFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEE 1027

Query: 721  SESIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLS 780
            SESIAENDD+KIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLS
Sbjct: 1028 SESIAENDDSKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLS 1087

Query: 781  PKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSE 840
            PKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSE
Sbjct: 1088 PKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSE 1147

Query: 841  IRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGEL 900
            IRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGEL
Sbjct: 1148 IRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGEL 1207

Query: 901  SSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSE 960
            SSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSE
Sbjct: 1208 SSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSE 1267

Query: 961  EYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSEL 1020
            EYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSEL
Sbjct: 1268 EYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSEL 1327

Query: 1021 HFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFD 1080
            HFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFD
Sbjct: 1328 HFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFD 1387

Query: 1081 MGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPA 1140
            MGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPA
Sbjct: 1388 MGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPA 1447

Query: 1141 TNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAE 1200
            TNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAE
Sbjct: 1448 TNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAE 1507

Query: 1201 KKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVP 1260
            KKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVP
Sbjct: 1508 KKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVP 1567

Query: 1261 DHWCMQQNLPGPWDLCGTADCASSNKTNANENVLSS 1295
            DHWCMQQNLPGPWDLCGTADCASSNKTNANENVLSS
Sbjct: 1568 DHWCMQQNLPGPWDLCGTADCASSNKTNANENVLSS 1603

BLAST of MELO3C013178 vs. NCBI nr
Match: XP_008447757.1 (PREDICTED: putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucumis melo])

HSP 1 Score: 2478.0 bits (6421), Expect = 0.0e+00
Identity = 1257/1294 (97.14%), Postives = 1257/1294 (97.14%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 60
            MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT
Sbjct: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 60

Query: 61   ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 120
            ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD
Sbjct: 61   ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 120

Query: 121  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 180
            AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV
Sbjct: 121  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 180

Query: 181  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP 240
            VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP
Sbjct: 181  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP 240

Query: 241  TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGESFLLT 300
            TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMG      
Sbjct: 241  TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMG------ 300

Query: 301  SPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVSHQST 360
                                           AVYQFDEEVRGSSDGENSVHEQSVSHQST
Sbjct: 301  -------------------------------AVYQFDEEVRGSSDGENSVHEQSVSHQST 360

Query: 361  LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV 420
            LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV
Sbjct: 361  LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV 420

Query: 421  PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW 480
            PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW
Sbjct: 421  PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW 480

Query: 481  MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF 540
            MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF
Sbjct: 481  MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF 540

Query: 541  LYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSEIA 600
            LYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSEIA
Sbjct: 541  LYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSEIA 600

Query: 601  HSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFHKF 660
            HSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFHKF
Sbjct: 601  HSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFHKF 660

Query: 661  LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEESE 720
            LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEESE
Sbjct: 661  LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEESE 720

Query: 721  SIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLSPK 780
            SIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLSPK
Sbjct: 721  SIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLSPK 780

Query: 781  KLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSEIR 840
            KLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSEIR
Sbjct: 781  KLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSEIR 840

Query: 841  QMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGELSS 900
            QMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGELSS
Sbjct: 841  QMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGELSS 900

Query: 901  IVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSEEY 960
            IVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSEEY
Sbjct: 901  IVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSEEY 960

Query: 961  RFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHF 1020
            RFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHF
Sbjct: 961  RFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHF 1020

Query: 1021 IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMG 1080
            IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMG
Sbjct: 1021 IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMG 1080

Query: 1081 NQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATN 1140
            NQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATN
Sbjct: 1081 NQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATN 1140

Query: 1141 NFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAEKK 1200
            NFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAEKK
Sbjct: 1141 NFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAEKK 1200

Query: 1201 ELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDH 1260
            ELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDH
Sbjct: 1201 ELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDH 1257

Query: 1261 WCMQQNLPGPWDLCGTADCASSNKTNANENVLSS 1295
            WCMQQNLPGPWDLCGTADCASSNKTNANENVLSS
Sbjct: 1261 WCMQQNLPGPWDLCGTADCASSNKTNANENVLSS 1257

BLAST of MELO3C013178 vs. NCBI nr
Match: XP_011658963.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucumis sativus] >KAE8646042.1 hypothetical protein Csa_016172 [Cucumis sativus])

HSP 1 Score: 2362.0 bits (6120), Expect = 0.0e+00
Identity = 1199/1294 (92.66%), Postives = 1234/1294 (95.36%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 60
            MVFKKHAAHKHMPTHCKNPKL+LIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT
Sbjct: 290  MVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 349

Query: 61   ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 120
            ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD
Sbjct: 350  ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 409

Query: 121  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 180
            AVYFQKIVEEHAGVLEGGKM TKTLMFIEGCPTRLGCTILLKGAHSDELKRVK+VVQCAV
Sbjct: 410  AVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKIVVQCAV 469

Query: 181  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP 240
            VMAF LILETSFIVDQ+AMFATIPFGGVP+ ASSD QSPLEPRSP VG+AESASD+ EQP
Sbjct: 470  VMAFHLILETSFIVDQRAMFATIPFGGVPATASSDRQSPLEPRSPNVGKAESASDKSEQP 529

Query: 241  TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGESFLLT 300
            TNI IS GSHEE S  SN+E  EKLI+SSEPEPYNPAIFSGFSSISDSLKR MGESFL +
Sbjct: 530  TNICISRGSHEETSTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESFLSS 589

Query: 301  SPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVSHQST 360
            SPY SLSSYFGH SDLSGLVAKS SIPST QAVYQFD EV+GSSD ENSVHE SVSHQST
Sbjct: 590  SPYQSLSSYFGHGSDLSGLVAKSGSIPSTPQAVYQFDVEVKGSSDEENSVHESSVSHQST 649

Query: 361  LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV 420
            LEGLGFHETA N + DTMQKK  LDSQSILVLMSSRNALKG +CEQSHFSHIVFYK+FDV
Sbjct: 650  LEGLGFHETALNYSVDTMQKKMSLDSQSILVLMSSRNALKGAICEQSHFSHIVFYKHFDV 709

Query: 421  PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW 480
            PLGKFLQENLLNQNNLCTVCGELPEAH Y++AHYRKQLSIQVKQLPGDKVLPGETEGKLW
Sbjct: 710  PLGKFLQENLLNQNNLCTVCGELPEAHIYFFAHYRKQLSIQVKQLPGDKVLPGETEGKLW 769

Query: 481  MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF 540
            MWSRCSKCKS GGPSKSTKRV ISTAARGLSFGKFLELCFSDDTL SKS VCGHSLFGDF
Sbjct: 770  MWSRCSKCKSTGGPSKSTKRVPISTAARGLSFGKFLELCFSDDTLLSKSPVCGHSLFGDF 829

Query: 541  LYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSEIA 600
            LYFFGLGNMVAM RYSEVAIYTVSMPPQKLEFNSSMRQGH I+E ENVYTKG+LLF+EIA
Sbjct: 830  LYFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHFIRETENVYTKGVLLFTEIA 889

Query: 601  HSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFHKF 660
            HSLKKIISER SSNVNHPQGF NDFSFVEEMLNEERSEFE+ IQNSLTKKGN D     F
Sbjct: 890  HSLKKIISERKSSNVNHPQGFPNDFSFVEEMLNEERSEFEINIQNSLTKKGNPD-----F 949

Query: 661  LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEESE 720
            LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSG+SE VEPEP++LKMNSNI+VGPEE E
Sbjct: 950  LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPEELE 1009

Query: 721  SIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLSPK 780
            SIAEND+TK+QQDIS+DENVLPLKEI VEGSDGESGGDELNLPSAIEVTEI IIDD SPK
Sbjct: 1010 SIAENDETKVQQDISLDENVLPLKEITVEGSDGESGGDELNLPSAIEVTEIPIIDDPSPK 1069

Query: 781  KLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSEIR 840
            KLSRQGTLSNGFD HHSDYEDSQVGRVLSSGDLH+DRTIPISIGDSTLSKLFCTPFSEIR
Sbjct: 1070 KLSRQGTLSNGFDYHHSDYEDSQVGRVLSSGDLHIDRTIPISIGDSTLSKLFCTPFSEIR 1129

Query: 841  QMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGELSS 900
            QMHLRDIQRSYFPELKSISSYTPKLL AASDFIHEEGQKLHIHLA+DNFVVSDYEGELSS
Sbjct: 1130 QMHLRDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHLANDNFVVSDYEGELSS 1189

Query: 901  IVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSEEY 960
            IVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDS+SSISSEEY
Sbjct: 1190 IVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISSEEY 1249

Query: 961  RFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHF 1020
            RFSSFDRLNLLDSLVSETFKRSD+EGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHF
Sbjct: 1250 RFSSFDRLNLLDSLVSETFKRSDYEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHF 1309

Query: 1021 IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMG 1080
            IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMG
Sbjct: 1310 IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMG 1369

Query: 1081 NQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATN 1140
            NQTCLAKV+GIYQVTVRE KSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATN
Sbjct: 1370 NQTCLAKVVGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATN 1429

Query: 1141 NFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAEKK 1200
            NFGEVLLDQNFVNDMNSSPLYVSNRA RRLQRAIWNDT FLNSINVMDYSLLVGVDAEKK
Sbjct: 1430 NFGEVLLDQNFVNDMNSSPLYVSNRAMRRLQRAIWNDTAFLNSINVMDYSLLVGVDAEKK 1489

Query: 1201 ELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDH 1260
            ELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTV+SPKEYKRRFRKFMSAHFLSVPDH
Sbjct: 1490 ELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSVPDH 1549

Query: 1261 WCMQQNLPGPWDLCGTADCASSNKTNANENVLSS 1295
            WCMQQNLPGPWDL  TAD ASS++TNA+EN  SS
Sbjct: 1550 WCMQQNLPGPWDLFDTADPASSHRTNADENDASS 1578

BLAST of MELO3C013178 vs. NCBI nr
Match: XP_038899624.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Benincasa hispida])

HSP 1 Score: 2331.2 bits (6040), Expect = 0.0e+00
Identity = 1178/1297 (90.82%), Postives = 1220/1297 (94.06%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 60
            MVFKKHAAHKHMPTHCKNPKL+LIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT
Sbjct: 297  MVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 356

Query: 61   ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 120
            ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRL+RIALCTGSPILTSETLMSQKTRQCD
Sbjct: 357  ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQKTRQCD 416

Query: 121  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 180
            AVYFQKIVEEHA VL+GG+  TKTLMFIEGCPTRLGCTILLKGAHSDELKRVK+VVQCAV
Sbjct: 417  AVYFQKIVEEHASVLDGGRRPTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKVVVQCAV 476

Query: 181  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP 240
            VMAF LILETSF+VDQ+AMFATIPFGGVP+I SSDP+SPLEP SP VGQAE+ASD+ EQP
Sbjct: 477  VMAFHLILETSFLVDQRAMFATIPFGGVPAIVSSDPRSPLEPCSPNVGQAENASDKSEQP 536

Query: 241  T---NIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGESF 300
            T   NI+ISSG+HEEASN SN+E+VEKLI+SSEPEPYNPAIFSGFSSI+DSLKR MGESF
Sbjct: 537  TDSMNIYISSGAHEEASNGSNLESVEKLIMSSEPEPYNPAIFSGFSSITDSLKRVMGESF 596

Query: 301  LLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVSH 360
            LL SPY SLSSYFGH SDLSGLV KSDSIPST QA  QFD EVRGSSD EN VHEQSVSH
Sbjct: 597  LLPSPYQSLSSYFGHGSDLSGLVPKSDSIPSTPQASDQFDVEVRGSSDEENLVHEQSVSH 656

Query: 361  QSTLEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKN 420
            QSTLE LGFHET PN + DTMQKK  LDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKN
Sbjct: 657  QSTLEALGFHETVPNYSEDTMQKKTSLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKN 716

Query: 421  FDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEG 480
            FDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLP DKVLPGETEG
Sbjct: 717  FDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPVDKVLPGETEG 776

Query: 481  KLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLF 540
            KLWMWSRCSKCKS GGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLF
Sbjct: 777  KLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLF 836

Query: 541  GDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFS 600
            GDFLYFFGLGNMVAMFRYSEVAIYT+SMPPQKLEFNSSMRQGHLI+E ENVYTKGMLLF+
Sbjct: 837  GDFLYFFGLGNMVAMFRYSEVAIYTISMPPQKLEFNSSMRQGHLIRETENVYTKGMLLFT 896

Query: 601  EIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAF 660
            EIAHSLKKIISE  SSN+NHPQGFLNDFS VEEMLNEERSEFEV IQNSL KKG+ D AF
Sbjct: 897  EIAHSLKKIISECKSSNLNHPQGFLNDFSLVEEMLNEERSEFEVNIQNSLGKKGSPDFAF 956

Query: 661  HKFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPE 720
            H++LNLNRLLWDLLLESCIWDRRLQSL SLGVT NSGT+ENVEPEPVMLKMNSNI+VGPE
Sbjct: 957  HRYLNLNRLLWDLLLESCIWDRRLQSLISLGVTINSGTTENVEPEPVMLKMNSNIDVGPE 1016

Query: 721  ESESIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDL 780
             SESI ENDDTK+Q DISVDENV P +E  VEGS GESGGDELNLP   EVTEI I+DDL
Sbjct: 1017 GSESIVENDDTKVQHDISVDENVSPQREAVVEGSIGESGGDELNLPLVTEVTEIPIMDDL 1076

Query: 781  SPKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFS 840
            SPK+LSRQGTL+NGFD  HSDYE+ QVGRVLSSGDLH+DRTIPIS GDSTLSKLF TPFS
Sbjct: 1077 SPKQLSRQGTLTNGFDYIHSDYENPQVGRVLSSGDLHLDRTIPISTGDSTLSKLFWTPFS 1136

Query: 841  EIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGE 900
            EIRQMH+RDIQRSYFPELKSISSYTPK L AASDFI+EEGQKLHI LA+DNF+VSDYEGE
Sbjct: 1137 EIRQMHIRDIQRSYFPELKSISSYTPKFLPAASDFINEEGQKLHIRLANDNFIVSDYEGE 1196

Query: 901  LSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISS 960
            LSSIVACVLALLKD P Q DSHNEDSKGEGGG  QPALSSRS NKVPSNGSSDSESSISS
Sbjct: 1197 LSSIVACVLALLKDLPLQADSHNEDSKGEGGGVSQPALSSRSQNKVPSNGSSDSESSISS 1256

Query: 961  EEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSE 1020
            EEYRFSSFDRLNLLDSLV ETFKR+DHEGVIKSLAKGKYLVNCPY NQFRDLRSRCCPSE
Sbjct: 1257 EEYRFSSFDRLNLLDSLVPETFKRADHEGVIKSLAKGKYLVNCPYFNQFRDLRSRCCPSE 1316

Query: 1021 LHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESF 1080
            LH+IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESF
Sbjct: 1317 LHYIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESF 1376

Query: 1081 DMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNP 1140
            DMGNQTCLAKVLGIYQVTVRE KSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHAR+NP
Sbjct: 1377 DMGNQTCLAKVLGIYQVTVRELKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARYNP 1436

Query: 1141 ATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDA 1200
            AT+NFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDT FLNSINVMDYSLLVGVDA
Sbjct: 1437 ATSNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTAFLNSINVMDYSLLVGVDA 1496

Query: 1201 EKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSV 1260
            EKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSV
Sbjct: 1497 EKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSV 1556

Query: 1261 PDHWCMQQNLPGPWDLCGTADCASSNKTNANENVLSS 1295
            PDHWCMQQNLPGPWDLCG  DCASS KTN  EN LSS
Sbjct: 1557 PDHWCMQQNLPGPWDLCGNTDCASSQKTNGEENDLSS 1593

BLAST of MELO3C013178 vs. NCBI nr
Match: XP_022932044.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D isoform X2 [Cucurbita moschata])

HSP 1 Score: 2006.9 bits (5198), Expect = 0.0e+00
Identity = 1032/1272 (81.13%), Postives = 1118/1272 (87.89%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 60
            MVFKKHAAHKHM T+ KNP+L+LIQG LGEAPIS LSSFNSMDQENDFTN+VIEMIE CT
Sbjct: 1    MVFKKHAAHKHMVTNWKNPRLILIQGRLGEAPISGLSSFNSMDQENDFTNYVIEMIETCT 60

Query: 61   ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 120
            ANVI+VEKTA+RVIQEAILK+ +TLVLDMKLHRL+RIALCTGSPILTSETL SQK RQCD
Sbjct: 61   ANVIIVEKTASRVIQEAILKRCITLVLDMKLHRLERIALCTGSPILTSETLASQKARQCD 120

Query: 121  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 180
            AVYFQK+V EH+G LEGGK STKTLMFIEGCP RLGCTILLKGA S ELKRVKLVVQ AV
Sbjct: 121  AVYFQKVV-EHSGGLEGGKRSTKTLMFIEGCPARLGCTILLKGARS-ELKRVKLVVQRAV 180

Query: 181  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQ- 240
            VMAF LILETSF+VDQ+AMFATIPFGGV ++ S DPQSPLEP S  + Q E A+D+ E  
Sbjct: 181  VMAFHLILETSFLVDQRAMFATIPFGGVSTMVSPDPQSPLEPCSSNILQVEKANDKSEPC 240

Query: 241  ---PTNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGES 300
                T+I+ISSG HEE+SN SN++ VEK I+ SE EPYNPAI SGFSSISDS K+ MGES
Sbjct: 241  VSLLTDIYISSGPHEESSNGSNLKPVEKPIIPSELEPYNPAILSGFSSISDSSKKVMGES 300

Query: 301  FLLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVS 360
            FLL+S + SLSSYFGHE D  GL  KSDSIPST QA  +FD +VRGSSD ENS HEQS S
Sbjct: 301  FLLSSTHHSLSSYFGHERDWIGLAPKSDSIPSTPQAANRFDVKVRGSSDEENSGHEQSAS 360

Query: 361  HQSTLEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYK 420
            HQS LEGLGFHE APN + +TMQKK  LDSQSILVLMSSRNALKGTMCEQSHF HIVFY+
Sbjct: 361  HQSALEGLGFHENAPNCSEETMQKKSSLDSQSILVLMSSRNALKGTMCEQSHFLHIVFYR 420

Query: 421  NFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETE 480
            NFDVPLGKFLQENLLNQ N CTVC ELPEAHFYY+AHY KQLSIQV+QLPG K+LPG+TE
Sbjct: 421  NFDVPLGKFLQENLLNQTNFCTVCSELPEAHFYYFAHYGKQLSIQVQQLPGYKLLPGQTE 480

Query: 481  GKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSL 540
            GKLWMWSRCSKCKS G PS STK VLISTAAR LSFGKFLELCFSD+TLPSKSS CGHSL
Sbjct: 481  GKLWMWSRCSKCKSKGEPSTSTKMVLISTAARSLSFGKFLELCFSDETLPSKSSGCGHSL 540

Query: 541  FGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLF 600
            FGDFLYFFGLGN VAMFRYS  AIYTVSMPPQKL+FNSSMRQGHLI+E ENV  KG LLF
Sbjct: 541  FGDFLYFFGLGNRVAMFRYSAAAIYTVSMPPQKLDFNSSMRQGHLIRETENVKKKGKLLF 600

Query: 601  SEIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLA 660
            +EIAHSLKKI SE  SSNV  PQ FLNDFS +EEMLN+ERSEFEVKIQNS        LA
Sbjct: 601  TEIAHSLKKISSECRSSNVK-PQCFLNDFSSIEEMLNQERSEFEVKIQNS--------LA 660

Query: 661  FHKFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGP 720
            FHK+L+LN+LLW+LLLESCIWDRRLQSL SLG+T+  GTSENVEPE V + MNSNI+   
Sbjct: 661  FHKYLSLNQLLWELLLESCIWDRRLQSLTSLGLTSTPGTSENVEPESVTMNMNSNIDARH 720

Query: 721  EESESIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDD 780
            EESESIA NDDT++QQDIS DENVLP+KEIAVEG DGESG DE + PS  EVTEI+I+DD
Sbjct: 721  EESESIAANDDTEVQQDISWDENVLPMKEIAVEGLDGESGCDENHSPSVTEVTEIRIMDD 780

Query: 781  LSPKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPF 840
            LSPK LSRQG+LSNGF+ HH D EDSQV RV SSGD+HVDRTIP+   DS+L KL   P 
Sbjct: 781  LSPKHLSRQGSLSNGFNQHHLDDEDSQV-RVASSGDMHVDRTIPVLTADSSLGKLLWAPC 840

Query: 841  SEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEG 900
             EIRQMH+RDIQRS+FPELKS SSYTPKL+ AAS F +EEGQKLHI LA DNF+VSDYEG
Sbjct: 841  VEIRQMHIRDIQRSFFPELKSFSSYTPKLIPAASAFTNEEGQKLHIRLAKDNFIVSDYEG 900

Query: 901  ELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSIS 960
            ELSSIVACVLALL+D   QTDSHNEDSKGEGGG  QP LSS+SLNKVPSNGSSDS+SS +
Sbjct: 901  ELSSIVACVLALLEDLSLQTDSHNEDSKGEGGGVSQPNLSSQSLNKVPSNGSSDSDSSAA 960

Query: 961  SEEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPS 1020
            S+ Y+FS+F+RLNLLDSLV ETFKR+DHEGVIKSLA GKY+VNCPY NQFRDLRS+CCPS
Sbjct: 961  SDAYQFSNFERLNLLDSLVPETFKRTDHEGVIKSLANGKYVVNCPYFNQFRDLRSQCCPS 1020

Query: 1021 ELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINES 1080
            ELH+IASLSRCI WNAKGGKS SFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYI +S
Sbjct: 1021 ELHYIASLSRCIKWNAKGGKSNSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYIKQS 1080

Query: 1081 FDMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFN 1140
            FDMGNQTCLAKVLGIYQV VRE KSGKEMRHD+MVMENLSFGRNIIRQYDLKGALHAR+N
Sbjct: 1081 FDMGNQTCLAKVLGIYQVAVREPKSGKEMRHDVMVMENLSFGRNIIRQYDLKGALHARYN 1140

Query: 1141 PATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVD 1200
            PA NNFGEVLLDQNFV DMNSSP+YVSNRAKRR+QRAIWNDT FLNSINVMDYSLLVGVD
Sbjct: 1141 PAANNFGEVLLDQNFVKDMNSSPIYVSNRAKRRMQRAIWNDTAFLNSINVMDYSLLVGVD 1200

Query: 1201 AEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLS 1260
            AEKKE VCGIIDYLRQYTWDKQLETWVKSSL+PKNVLPTV+SPKEYKRRFRKFMSAHFLS
Sbjct: 1201 AEKKEFVCGIIDYLRQYTWDKQLETWVKSSLVPKNVLPTVISPKEYKRRFRKFMSAHFLS 1260

Query: 1261 VPDHWCMQQNLP 1269
            VPDHWC+QQN P
Sbjct: 1261 VPDHWCLQQNFP 1260

BLAST of MELO3C013178 vs. ExPASy Swiss-Prot
Match: Q9XID0 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thaliana OX=3702 GN=FAB1D PE=3 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 2.1e-297
Identity = 612/1294 (47.30%), Postives = 797/1294 (61.59%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQEND----FTNHVIEMI 60
            +VFKKHAA KHM T  ++P+++L++G+LG  PIS  SS  S++Q+N+    +   V+++I
Sbjct: 230  LVFKKHAALKHMATKYEHPRIMLVEGVLGH-PISGFSSLQSVNQDNEYLLKYVKPVVDII 289

Query: 61   EGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKT 120
            E    +V+LVEK+ +R IQ+ IL KG+TLV DMKLHRLQRI+ C GSPIL+ ++L SQK 
Sbjct: 290  EASKPDVMLVEKSVSRDIQKTILDKGVTLVFDMKLHRLQRISRCIGSPILSVDSLSSQKL 349

Query: 121  RQCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVV 180
            + CD+   +KIVEEH    E  K  TKTLMF+EGCPTRLGCTILLKG HS+ LK+VK VV
Sbjct: 350  KHCDSFRIEKIVEEHNAAGESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVKEVV 409

Query: 181  QCAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQ 240
            Q + ++A+ L+LE SF+ D+  MF+TI      S         +E  SP     ES S+ 
Sbjct: 410  QYSFILAYHLMLEASFLADRHTMFSTIFAKEATSCVVE-----IENFSPSPSPRESPSEA 469

Query: 241  IEQPTNIHISSGSHEEA---SNESNVETVEKLIVSSE----PEPYNPAIFSGFSSISDSL 300
            ++ P    +S+G  E+    + E++ E V       +     EPYNP IF+GFSS+S  L
Sbjct: 470  VDIP----VSNGFDEQTIQINGEADGEKVGTWESDGDHVFSHEPYNPVIFTGFSSLSARL 529

Query: 301  KRAMGESFLLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENS 360
                       S YL             G V   +S+P ++         +    + E  
Sbjct: 530  -----------SKYL-------------GFVQNPESVPVSVDTDVSTTSNLDSIRESEED 589

Query: 361  VHEQSVSHQSTLEGLGFHETAPNDTGDTMQK-----KPPLDSQSILVLMSSRNALKGTMC 420
              E++   Q  L        + +D GD   +     +  L+SQSILVL+S RNAL+G MC
Sbjct: 590  TAEKNEDKQPLLLDPELPVNSSSDDGDNKSQTENDIESTLESQSILVLVSKRNALRGIMC 649

Query: 421  EQSHFSHIVFYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQ 480
            +Q HFSHI FYK+FDVPL KFL++ + NQ NLC  C E PEAH YYYAH  KQL+IQ+K+
Sbjct: 650  DQRHFSHIKFYKHFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLTIQIKR 709

Query: 481  LPGDKVLPGETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDT 540
            +P  K L GE +GK+WMWSRC KCK+     KSTKRVLISTAAR LSFGKFLEL FS  T
Sbjct: 710  IPVAKGLAGEAKGKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELSFSQQT 769

Query: 541  LPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKE 600
              ++SS CGHS   DFL+FFGLG+MVAM  YS+VA YTVS+PP KLE +  ++ G L KE
Sbjct: 770  FLNRSSSCGHSFDSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAGWLEKE 829

Query: 601  IENVYTKGMLLFSEIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQ 660
             + V+TKG+ LF + A  LK++ S+  +S++ + Q      S +EE+L  ER  FE  I+
Sbjct: 830  FQTVFTKGISLFEDAAGFLKRLRSQFTNSDLRY-QRARKLLSNIEELLKHERCIFEENIK 889

Query: 661  NSLTKKGNLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPV 720
            NS  K   +D   H+ L LNR+ W+LLL++ IW+ RLQSL           S+ + P   
Sbjct: 890  NSFDKAKTIDDVSHRLLRLNRMRWELLLQALIWNYRLQSLV---------LSDRLLPSSD 949

Query: 721  MLKMNSNINVGPEESESIAENDDTKIQQDIS---VDENVLPLKEIAVEGSDGESGGDELN 780
              K+         E+      +D K+    S   +D  ++  K+I + G+      D++ 
Sbjct: 950  ETKIYEQGLKTVSEAGMTRYENDNKVSDSGSNGGIDTPLVEHKDIPIAGA-SVGDNDQMA 1009

Query: 781  LPSAIEVTEIQIIDDLSPKKLSRQGTLSNGFDCH-----HSDYEDSQVGRVLSSGDLHVD 840
                 E  E Q +   SP   S    ++N FD H     HS         +  +G+  +D
Sbjct: 1010 ESYVPEDNESQTLCSSSPDTTS---PINNHFDTHLAVNVHSTNGQEADKSIPVTGE-SLD 1069

Query: 841  RTIPISIGDSTL--SKLFCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIH 900
              +  S G   L   + F  PF E+R   + DI++ Y  + + ++++T + L   +  I 
Sbjct: 1070 DEVSTSNGPHILGWDEWFWLPFEELRSKRIVDIEKEYLLKFEYVNNFTQENLQTVNQIIT 1129

Query: 901  EEGQKLHIHLADDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPA 960
            EE  +L I L DD+F+VSDYE ELSS++AC LA L         +NE+SK      +  +
Sbjct: 1130 EESSRLRISLRDDDFIVSDYEDELSSLIACALAHL---------NNEESKKPLSRCIHGS 1189

Query: 961  LSSRSLNKVPSNGSSDSESSISSEEYRFSSFDRLNLL-DSLVSETFKRSDHEGVIKSLAK 1020
            L     N   S  +    S  SSE     S +RL  L    V  TF      G +KS+ K
Sbjct: 1190 LQGFLDNNQDSKQTDRDVSRFSSE-----STNRLETLPPPEVLVTF------GSVKSVGK 1249

Query: 1021 GKYLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKE 1080
             KY +   Y + FRDLR RCC SEL +IASLSRC  W+AKGGKSKS FAKTLDDRFI+KE
Sbjct: 1250 PKYSIVSLYADDFRDLRKRCCSSELDYIASLSRCKPWDAKGGKSKSVFAKTLDDRFIVKE 1309

Query: 1081 IKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTVREQK-SGKEMRHDLMVM 1140
            IK+TEY+SF+ FA EYFKY+ +S+D+GNQTCLAKVLGI+QVTVR+ K  GKE+RHDLMVM
Sbjct: 1310 IKKTEYESFVTFATEYFKYMKDSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIRHDLMVM 1369

Query: 1141 ENLSFGRNIIRQYDLKGALHARFN-PATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQ 1200
            ENLSF R + RQYDLKGALHARF   + N   +VLLDQNFVNDMN SPLYVS  +K+ LQ
Sbjct: 1370 ENLSFSRKVTRQYDLKGALHARFTATSANGEDDVLLDQNFVNDMNKSPLYVSKTSKQNLQ 1429

Query: 1201 RAIWNDTTFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSL-IPK 1260
            RA++NDT+FL SINVMDYSLLVGVD E  ELVCGIIDYLRQYTWDKQLETWVKSSL +PK
Sbjct: 1430 RAVYNDTSFLTSINVMDYSLLVGVDDENHELVCGIIDYLRQYTWDKQLETWVKSSLVVPK 1453

Query: 1261 NVLPTVVSPKEYKRRFRKFMSAHFLSVPDHWCMQ 1265
            NV PTV+SP +YK RFRKFM  HFL VPD WC Q
Sbjct: 1490 NVQPTVISPIDYKTRFRKFMKTHFLCVPDQWCDQ 1453

BLAST of MELO3C013178 vs. ExPASy Swiss-Prot
Match: Q9LUM0 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=3702 GN=FAB1B PE=2 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 2.5e-197
Identity = 485/1338 (36.25%), Postives = 710/1338 (53.06%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPIS-RLSSFNS-MDQENDFTNHVIEMIEG 60
            +V KK+  ++ M T  +  +L+++ G L    +S +LSSF++ + QE D     +  I  
Sbjct: 459  VVCKKNVVNRRMSTKIEKARLLILGGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHA 518

Query: 61   CTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTS-ETLMSQKTR 120
               N++LVEK+ +R  QE +L K ++LVL++K   L RIA CTG+ I+ S + L SQK  
Sbjct: 519  ERPNILLVEKSVSRFAQEYLLAKDISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLG 578

Query: 121  QCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQ 180
             C+     +  EEH    + GK   KTLM+ E CP  LG TILL+GA+ DELK+VK VVQ
Sbjct: 579  YCENFRVDRYPEEHGSTGQVGKKVVKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQ 638

Query: 181  CAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQI 240
              V  A+ L LETSF+ D+ A    +P     ++A  D  + +E RS       + S   
Sbjct: 639  YGVFAAYHLALETSFLADEGAS-PELPLNSPITVALPDKSTSIE-RSISTVPGFTVSTYE 698

Query: 241  EQPTNIHISSGSHEEASNESN-VETVEKLIVSSE--PEPYNPAIFSGFSSISDSLKRAMG 300
            + PT +  +      +   S  + T   L +  +  P PY     SG+ +      R + 
Sbjct: 699  KSPTMLSCAEPQRANSVPVSELLSTTTNLSIQKDIPPIPYG----SGWQA------REIN 758

Query: 301  ESFLLTSPYLSLS----SYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGS--SDGEN 360
             SF+ +   +SL+          SDLSG     D+       +   DE    S    G+ 
Sbjct: 759  PSFVFSRHNISLNLPDRVIESRNSDLSGRSVPVDTPADKSNPIVVADETTNNSLHLSGQG 818

Query: 361  SVHEQS-------VSHQSTLEGLGF----HETAPNDTGDTMQKKP--PLDSQSILVLMSS 420
             V + S       V +Q     L      +   P +T    ++ P  P D QSILV +SS
Sbjct: 819  FVRKSSQIGTSIMVENQDNGSELTIAQQQNNEKPKETQSQKEEFPPSPSDHQSILVSLSS 878

Query: 421  RNALKGTMCEQSHFSHIVFYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYR 480
            R+  KGT+CE+SH   I +Y +FD PLG+FL+++L +Q+  C  C    EAH + Y H +
Sbjct: 879  RSVWKGTVCERSHLFRIKYYGSFDKPLGRFLRDHLFDQSYRCRSCEMPSEAHVHCYTHRQ 938

Query: 481  KQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKF 540
              L+I VK+L  D +LPGE EGK+WMW RC +C  L G   +T RV++S AA GLSFGKF
Sbjct: 939  GSLTISVKKLQ-DYLLPGEKEGKIWMWHRCLRCPRLNGFPPATLRVVMSDAAWGLSFGKF 998

Query: 541  LELCFSDDTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSS 600
            LEL FS+    S+ + CGHSL  D L F+G GNMVA FRY+ + +++V +PP  L FN  
Sbjct: 999  LELSFSNHAAASRVACCGHSLHRDCLRFYGFGNMVACFRYATIDVHSVYLPPSILSFNYE 1058

Query: 601  MRQGHLIKEIENVYTKGMLLFSEIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEE 660
              Q  + +E + V  +  LLFSE+ +++ +I            +GF      +EE+L +E
Sbjct: 1059 -NQDWIQRETDEVIERAELLFSEVLNAISQIAE----------KGFRRRIGELEEVLQKE 1118

Query: 661  RSEFEVKIQNSLTKKGNLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGT 720
            ++EFE  +Q  L ++ N        L L R+   LL +S +WD RL + ++L    NS  
Sbjct: 1119 KAEFEENMQKILHREVNEGQPLVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDD 1178

Query: 721  SENVEPE----------PVM------LKMNSNINVGPEESESIAENDDTKIQQDISVDEN 780
            ++  E E          P M      L   S +N+ P+   +        +Q++   + +
Sbjct: 1179 TKREENEKPPLAKSQTLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGSLNNVQKEADTNSD 1238

Query: 781  VLPLKEIAVEGSDGESGGD-ELNLPSAIEVTEIQIIDDLSPKKLS----------RQGTL 840
            +   K+   E S  ++  D    L + ++V   Q    +  K LS          RQ ++
Sbjct: 1239 LYQEKDDGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQIVMKNLSATLDAAWIGERQTSV 1298

Query: 841  ----SNGFDCHHSDYEDSQVGRVLSSGDLHVDR-------TIPISIGDSTLSKLFCTPFS 900
                +N      S   +S     +S G + +D         +   +  +  SK +     
Sbjct: 1299 EIPTNNKVSLPPSTMSNSSTFPPISEGLMPIDLPEQQNEFKVAYPVSPALPSKNYENSED 1358

Query: 901  EIRQMHL------RDIQRSYF---PELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDN 960
             +  + +      R I +++     +L +   ++P  +++  +   + G +L + +  ++
Sbjct: 1359 SVSWLSVPFLNFYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLND 1418

Query: 961  FVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQP-ALSSRSLNKVPSNG 1020
             VV  Y+ E +S++A  L +  +   QT +  E           P  +     +   SNG
Sbjct: 1419 IVVPVYDDEPTSMIAYAL-MSPEYQRQTSAEGESLVSYPSELNIPRPVDDTIFDPSRSNG 1478

Query: 1021 SSDSESSISSEEYRFSSFDRLNLLDSL-VSETFKRSDHEGVIKSLAKGKYLVNCPYINQF 1080
            S D ES +S    R +S     LLD L  ++        G   +L K KY V C Y  +F
Sbjct: 1479 SVD-ESILSISSSRSTS-----LLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRF 1538

Query: 1081 RDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFA 1140
              LR  C PSEL +I SLSRC  W A+GGKS  FFAKTLDDRFIIK++ +TE +SF+KFA
Sbjct: 1539 EALRGICLPSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFA 1598

Query: 1141 PEYFKYINESFDMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYD 1200
            P YFKY++ES    + TCLAK+LGIYQV  ++ KSGKE + D+++MENL FGR + R YD
Sbjct: 1599 PAYFKYLSESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYD 1658

Query: 1201 LKGALHARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINV 1260
            LKG+  AR+NP ++   +VLLDQN +  M +SP++V N+AKR L+RA+WNDT FL   +V
Sbjct: 1659 LKGSSRARYNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDV 1718

Query: 1261 MDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLI---PKNVLPTVVSPKEYK 1262
            MDYSLLVGVD EK ELV GIID+LRQYTWDK LE+WVK + I   PKN  PTV+SPK+YK
Sbjct: 1719 MDYSLLVGVDEEKNELVLGIIDFLRQYTWDKHLESWVKFTGILGGPKNEAPTVISPKQYK 1765

BLAST of MELO3C013178 vs. ExPASy Swiss-Prot
Match: Q0WUR5 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=3702 GN=FAB1A PE=2 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 2.8e-188
Identity = 473/1349 (35.06%), Postives = 694/1349 (51.45%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPIS-RLSSFNS-MDQENDFTNHVIEMIEG 60
            +V KK+ AH+ M +  + P+L+++ G L    IS +LSSF++ + QE D     +  I+ 
Sbjct: 447  VVCKKNVAHRRMTSKIEKPRLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDS 506

Query: 61   CTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTS-ETLMSQKTR 120
               +++LVEK+ +R  QE +L K ++LVL++K   L+RI+ CTG+ I+ S + L S K  
Sbjct: 507  HNPDILLVEKSVSRFAQEYLLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLG 566

Query: 121  QCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQ 180
             CD  + +K VE H    +  K   KTLMF +GCP  LGCTILLKGAH DELK+VK V+Q
Sbjct: 567  YCDLFHVEKFVETHVSPCQVAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQ 626

Query: 181  CAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPL----------------- 240
              V  A+ L LETSF+ D+ A    +P     ++A  D  S +                 
Sbjct: 627  YGVFAAYHLALETSFLADEGASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEK 686

Query: 241  EPRSPKVGQAESASDQIEQPTNIHISSGSHEEASNESN--VETVEKLIVSSEPEPYNPAI 300
             P +   G+   A+  +   T    SS +H +   + N  ++  E+L+ + +     P  
Sbjct: 687  SPTTELRGEPHKANGDL---TGNFTSSKTHFQGKLDGNDRIDPSERLLHNLDTVYCKPP- 746

Query: 301  FSGFSSISDSLKRAMGE---SFLLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQ 360
                +S  D L   +     SF +  P +    +    S LSG      +        Y 
Sbjct: 747  -ETITSKDDGLVPTLESRQLSFHVEEPSVQKDQW----SVLSG------ATEQVTDGGYT 806

Query: 361  FDEEVRGSSDGENSVHEQSVSHQSTLEGL--GFHETAPNDTGDTMQKKPPLDSQSILVLM 420
             D  V G+         Q+ + Q  +E     FH +A              D QSILV +
Sbjct: 807  NDSAVIGN---------QNFNRQEQMESSKGDFHPSAS-------------DHQSILVSL 866

Query: 421  SSRNALKGTMCEQSHFSHIVFYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAH 480
            S+R   KG++CE++H   I +Y +FD PLG+FL++NL +Q+  C  C    EAH + Y H
Sbjct: 867  STRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCTMPAEAHIHCYTH 926

Query: 481  YRKQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFG 540
             +  L+I VK+LP  ++LPG+ EGK+WMW RC KC  + G   +T+R+++S AA GLSFG
Sbjct: 927  RQGSLTISVKKLP--ELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVMSDAAWGLSFG 986

Query: 541  KFLELCFSDDTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFN 600
            KFLEL FS+    S+ + CGHSL  D L F+G G MVA FRY+ + IY V++PP KL FN
Sbjct: 987  KFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAVTLPPAKLYFN 1046

Query: 601  SSMRQGHLIKEIENVYTKGMLLFSEIAHSLKKIISER--NSSNVNHPQGFLNDFSFVEEM 660
                Q  L KE + V  K  +LF+E+  +L +I ++     S  + P         +  +
Sbjct: 1047 YE-NQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIKLSLEELAGL 1106

Query: 661  LNEERSEFEVKIQNSL--TKKGNLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSLASL-- 720
            L + + E++  +Q  L   K G   +     L +N+L   ++ +S  WD  L   A++  
Sbjct: 1107 LEQRKKEYKDSLQQMLNVVKDGQPTI---DILLINKLRRLIIFDSYAWDECLAGAANMVR 1166

Query: 721  -----------------GVTTNSGTSENVEPEPV-------MLKMNSNINVGPEESESIA 780
                              V+    + E V+  P         L  +++      + +S A
Sbjct: 1167 NNYLEAPKNSAPKVMGRNVSLEKLSDEKVKSIPTHVAICNDSLLQDADYETCLNQGKSFA 1226

Query: 781  ENDDT-KIQQDISVDENVLPLKEIAVEGSDGESGGDEL----------NLPSAIEVTEIQ 840
            +      I +D+  D       +  +E    E G D            +  S  + T++ 
Sbjct: 1227 DTSGKFAIPEDVGSDRP----PDCRMEFDPSEGGKDNFVESSQVVKPAHTESQFQATDLS 1286

Query: 841  IIDDLSPKKLSRQGTLSNGF---DCHHSDYEDSQVG--RVLSS--------GDLHVDRTI 900
              D L    +  Q T  NG        +    +Q+   R+L S        G  + + T 
Sbjct: 1287 --DTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIPDLRLLGSESELNFKGGPTNDEHTT 1346

Query: 901  PISIGDSTLSKLFCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQK 960
             + +   +        +S   + H+    R  +     +SSY         +     G +
Sbjct: 1347 QVQLPSPSFYYSLNKNYSLNSRKHIMAEDRPVY-----VSSY--------RELEWRSGAR 1406

Query: 961  LHIHLADDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGG--ALQPALSS 1020
            L + L  ++ V+  Y+ E +SI+A  L   + +   + S     + + GG  +L  +++ 
Sbjct: 1407 LLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNL 1466

Query: 1021 RSLNKVPSNGSSDSESSISSEEYRFSSF--DRLNLLDSLVSETFKRSDHEGVIKSLAKGK 1080
             SLN + S+ S D   S+SS + + S      L L D     +F      G      K K
Sbjct: 1467 LSLNSL-SDLSVDMSRSLSSADEQVSQLLHSSLYLKDLHARISFTDEGPPG------KVK 1526

Query: 1081 YLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIK 1140
            Y V C Y  +F  LR  CCPSE  FI SL RC  W A+GGKS  FFAK+LDDRFIIK++ 
Sbjct: 1527 YSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVT 1586

Query: 1141 RTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENL 1200
            +TE +SF+KF P YFKY+ ES    + T LAK+LGIYQV+ +  K GKE + D++VMENL
Sbjct: 1587 KTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENL 1646

Query: 1201 SFGRNIIRQYDLKGALHARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIW 1260
             F RN  R YDLKG+  AR+NP T+    VLLDQN V  M +SP++V ++AKR L+RA+W
Sbjct: 1647 LFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSPIFVGSKAKRLLERAVW 1706

Query: 1261 NDTTFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLI---PKNV 1262
            NDT+FL SI+VMDYSLLVGVD E+ ELV GIID++RQYTWDK LETWVK+S +   PKN 
Sbjct: 1707 NDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHLETWVKTSGLLGGPKNS 1726

BLAST of MELO3C013178 vs. ExPASy Swiss-Prot
Match: Q9SSJ8 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thaliana OX=3702 GN=FAB1C PE=2 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 2.8e-188
Identity = 469/1302 (36.02%), Postives = 699/1302 (53.69%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPIS-RLSSFNS-MDQENDFTNHVIEMIEG 60
            +V  K+  HK M +  KNP+++L+ G L    ++ +L+SFN+ + QEN+    +I  IE 
Sbjct: 403  IVCSKNITHKRMISQYKNPRVMLLAGSLEYQRVAGQLASFNTLLQQENEHMKAIIAKIES 462

Query: 61   CTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTS-ETLMSQKTR 120
               NV+LVEK+A+   Q+ +L+K ++LVL++K   L RIA CTG+ +  S +++ + +  
Sbjct: 463  LRPNVLLVEKSASSYAQQYLLEKEISLVLNVKRSLLDRIARCTGAVLCPSLDSISTARLG 522

Query: 121  QCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQ 180
             C+    ++++E+H    +  +  ++TLM+ EGCP RLGCT++L+G+  +ELK+VK V+Q
Sbjct: 523  HCELFRTERVLEQHEAGNQSNRKPSRTLMYFEGCPRRLGCTVVLRGSCREELKKVKHVIQ 582

Query: 181  CAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQI 240
             AV  A+ L LETSF+ D+ A    I       + ++  +  ++     + Q+ + +D  
Sbjct: 583  YAVFAAYHLSLETSFLADEGASLPKIRLKQPGMVRTASQRRIIDEGISLITQSPTETD-- 642

Query: 241  EQPTNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPA-IFSGFSSISDSLKRAMGES 300
               +   + + +HE+   E      E  +  S  E ++P  IF   S +       +   
Sbjct: 643  ---SQALLETAAHED---EHTAPMPEHEVCESLCEDFDPTQIFPPSSEVETEQSDTLNGD 702

Query: 301  F---LLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQ 360
            F   L+T  Y S      HE  L      S  IP T        EE  G  + EN    Q
Sbjct: 703  FANNLVTRSYSSNQLNDLHEPTL----CLSSEIPET-PTQQPSGEEDNGRGEEEN----Q 762

Query: 361  SVSHQSTLEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIV 420
             V+ Q     L  HE+   D   +         QSILV  SSR  LK ++CE+S    I 
Sbjct: 763  LVNPQD----LPQHESFYEDDVSSEYFSAADSHQSILVSFSSRCVLKESVCERSRLLRIK 822

Query: 421  FYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPG 480
            FY +FD PLG++L+++L ++ + C  C EL +AH   Y+H    L+I V++LP  K LPG
Sbjct: 823  FYGSFDKPLGRYLKDDLFDKTSSCRSCKELVDAHVLCYSHQNGNLTINVRRLPSMK-LPG 882

Query: 481  ETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCG 540
            E +GK+WMW RC +C  + G   +T+RV++S AA GLSFGKFLEL FS+    ++ + CG
Sbjct: 883  EQDGKIWMWHRCLRCAHVDGVPPATRRVVMSDAAWGLSFGKFLELSFSNHATANRVASCG 942

Query: 541  HSLFGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGM 600
            HSL  D L F+G GNMVA FRYS + I TV +PP  LEFNS  +Q  +  E   +  K  
Sbjct: 943  HSLQRDCLRFYGFGNMVAFFRYSPINILTVLLPPSMLEFNSHPQQEWIRTEAAELVGKMR 1002

Query: 601  LLFSEIAHSLKKIISERNSSNVNHPQGFLNDFSF----VEEMLNEERSEFEVKIQ----N 660
             +++EI+  L ++  E  SS +   Q    D       + + L +E+ E++  +Q     
Sbjct: 1003 TMYTEISDMLNRM--EEKSSLLEPEQSEACDLHSRIIGLIDQLVKEKDEYDDALQPIFEE 1062

Query: 661  SLTKKGNLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSL------ASLGVTTNSGTSENV 720
            +L  +G+LD+     L LNRL   L++ +  WD +L  L      AS+  T +     N 
Sbjct: 1063 NLQIQGSLDI-----LELNRLRRALMIGAHAWDHQLYLLNSQLKKASVFKTGDDNAPRNP 1122

Query: 721  E---PEPVMLKMNSNINVGPEESESIAE-NDDTKIQQDISVDENVLPLKEIAVEGSDGES 780
            E   P  +  +M    +   E+S + +E N D K  ++I      L       E  D   
Sbjct: 1123 EMHDPPKIDRRMQEGSDERDEQSHTDSEANGDNKDPENIPSPGTSLS------ERIDSAW 1182

Query: 781  GGDELNLPSAIEVTEIQIID--DLSPKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDL 840
             G   NL  A  + E +     + S ++L+R   + + FD      E  Q G   SS  L
Sbjct: 1183 LGSFQNLEKAETIAETEGFSAVNSSLRRLARPIRVQS-FDSAIRFQERIQKGLPPSSLYL 1242

Query: 841  HVDRTIPISIGDSTLSKLFCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFI 900
               R+   S        +   P S + + + + +      +L  I    P  +++AS   
Sbjct: 1243 STLRSFHAS---GEYRNMVRDPVSNVMRTYSQMLPLE-VQKLDLIVGSAPTYISSASQM- 1302

Query: 901  HEEGQKLHI-HLADDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQ 960
              +G ++ I     ++ VV  Y+ + +S+V+  +         +  + E    +G   L 
Sbjct: 1303 -ADGARMLIPQRGLNDIVVPVYDDDPASVVSYAI--------NSKEYKEWIVNKG---LA 1362

Query: 961  PALSSRSLNKVPSNGSSDSE--------SSISSEEYRFSSFDRLNLLDSLVSETFKRSDH 1020
             + SS +LN   S  S+ S           I    Y  S  DR +        T   SD 
Sbjct: 1363 SSSSSSNLNNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQDDRKS-----PHLTISFSDR 1422

Query: 1021 EGVIKSLAKG--KYLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFA 1080
                 +  +G  K+ V C +  QF  LR  CCPSE+ F+ SLSRC  W+A+GGKS  +FA
Sbjct: 1423 ASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRCQRWSAQGGKSNVYFA 1482

Query: 1081 KTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTVREQKSG 1140
            K+LD+RFIIK++ +TE DSF  FAPEYFKY+ ES   G+ TCLAK+LGIYQV+++  K G
Sbjct: 1483 KSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAKILGIYQVSIKHPKGG 1542

Query: 1141 KEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFGEVLLDQNFVNDMNSSPLYV 1200
            KE + DLMVMENL + R I R YDLKG+  +R+NP T+   +VLLD N +  + + P+++
Sbjct: 1543 KETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLLDMNLLETLRTEPIFL 1602

Query: 1201 SNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETW 1260
             ++AKR L+RAIWNDT FL S++VMDYSLLVG D E+KELV GIID++RQYTWDK LETW
Sbjct: 1603 GSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGIIDFMRQYTWDKHLETW 1646

Query: 1261 VKSSLI---PKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDHW 1262
            VK+S I   PKN  PT+VSPK+YKRRFRK M+ +FL+VP+ W
Sbjct: 1663 VKASGILGGPKNASPTIVSPKQYKRRFRKAMTTYFLTVPEPW 1646

BLAST of MELO3C013178 vs. ExPASy Swiss-Prot
Match: O59722 (1-phosphatidylinositol 3-phosphate 5-kinase fab1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=fab1 PE=1 SV=2)

HSP 1 Score: 305.8 bits (782), Expect = 2.3e-81
Identity = 354/1434 (24.69%), Postives = 602/1434 (41.98%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISR--LSSFNSMDQENDFTNHVIEMIEG 60
            ++F K A+ K M    + P++ L+   L  A   +  LS    + Q+ ++  +++  I  
Sbjct: 544  VLFSKKASSKSMDRSLRRPRIALLTFSLDYACDEQRILSLDLIISQQEEYIINLVNRICM 603

Query: 61   CTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQ 120
               N++  +     +  +   + G+     +K   L  IA C  + I++S   +S   R 
Sbjct: 604  LKPNLVFAQGQIPSIALKYFEEHGVIAFHGLKESVLYDIARCCRADIISSIDKLSLCPRL 663

Query: 121  CDAVYFQ---KIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLV 180
                 FQ    +V+E+ G+        KT   ++ C  RLGCTI+L+GA  ++L +VK +
Sbjct: 664  GTCGRFQLRTYVVDENKGL-------RKTFAILDRCSERLGCTIVLRGADYNQLSKVKKI 723

Query: 181  VQCAVVMAFQLILETSFIVDQ--------QAMFATIPFGGVPSIASSDPQSPLEPRSPKV 240
            V+  V++A+ + LE + + D+        +  + ++    +PS AS+           K 
Sbjct: 724  VELVVLIAYHIKLECALLRDKFVNMPELFETTYQSLSRKSLPSFASTAADKEKSQNHEK- 783

Query: 241  GQAESASDQIEQPTNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISD 300
             ++ ++ +Q  +P      S S  + SN S +E +  L  S +           +SSI+ 
Sbjct: 784  -KSLNSDNQSLRPLENENQSVSSTQGSN-SPLELINNLPASDD-----------YSSITK 843

Query: 301  SLKRAMGESFLLTSPYLS-----LSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRG 360
            +LK      FL  SP+LS     L +   +   +   + K       L         V  
Sbjct: 844  ALK----TRFLTFSPFLSKPLPRLLNQVNYYQFIRNKLLKD----VKLHPYSPTGSFVMK 903

Query: 361  SSDGENSVHEQSVSHQSTLEGLGFHETAPNDTGDTMQKK---PPLDSQSILVLMSSRNAL 420
             S+ +N       S++       +H      T      K    P  SQ I++L S  N  
Sbjct: 904  QSENDNVEESYEESYKFFCIDERYHFLEKQWTLYYSHSKLMFSPFSSQRIILLYSIINKE 963

Query: 421  KGTMCEQSHFSHIVFYKNFDVPLGKFLQENLLNQNNLCTVCGELPEA-----HFYYYAHY 480
                C       + FY+  D  LG++++++ LN N  C   GE  +      H+  Y H 
Sbjct: 964  TSVPCIGPERCLLEFYRETDCTLGQYIEDSCLNTNVSCG--GEYCKTNDMLWHYRSYVHG 1023

Query: 481  RKQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGK 540
              ++S+ ++       +PG  E K+ MWS C  CK      K+T   ++S      SFGK
Sbjct: 1024 NSRISVFLESF--SCPVPG-LEEKIIMWSYCKFCK------KNTHITVMSEETWKYSFGK 1083

Query: 541  FLELCFSDDTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNS 600
            +LE  F +  +  +   C HS+    +++FG  NM   F+   + I+ + +P   L  N 
Sbjct: 1084 YLEFMFYNSQIRDRFEFCDHSVMAQHVHYFGYCNMALRFQRDLIEIFELFVPSVTLRNNP 1143

Query: 601  SMRQGHLIKEI-ENVYTKGMLLFSEIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLN 660
            S      IKE+ E  Y +   +  +   S+   I++     V  P+ F        E   
Sbjct: 1144 S-----YIKELKEKEYKRLKGVIEKCLSSVASRINQIKCDWVTDPEKF--------ESCT 1203

Query: 661  EERSEFEVKIQNSLTKKGN------LDLAFHKFLNLNRLLWDLLLESCIWDRRLQSLASL 720
             E S+F   + +  T+  +      L+ +   +L+LN +L  L  +   W++R      L
Sbjct: 1204 SEISKFRTLLSSDYTELYSEFDSIYLNSSTSDYLSLNSILRVLQGKMVKWEQRFLDYQRL 1263

Query: 721  GVTTNSGTSE--NVEPEPVMLKM--------------NSNINVGPE-ESESIAENDDTKI 780
             + +    S+    + + V L+               N+ I++ P  ++ES  +  +   
Sbjct: 1264 YLPSYKELSKIAAAQIKKVFLERPLSQTPLDLPETLENTQIDIYPSFKTESTDDQLEKVT 1323

Query: 781  QQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLSPKKLSRQGTLSN 840
            Q +++ ++ V P  +        ES    +   S I    I   +D+S +       +SN
Sbjct: 1324 QTNVASNKRVAPYADSMANVGSPESDCFSVATSSDIPKANIDFTNDISTQNTFPASPVSN 1383

Query: 841  -GFD---------------CHHSDYEDSQVGR--VLSSGDLHVDRTIPISIGDSTLSKLF 900
             GF                  H     S   R  V +S    ++  I  SI     S   
Sbjct: 1384 SGFSRQTYPNISQRQGVNMLSHKRKSASTSDRRFVNASSTSGMNMPISSSISAKISSIQN 1443

Query: 901  CTPFSEIRQMHLRDIQRSYFP--------ELKSISSYTPKLLTA-------------ASD 960
             T +S  + +  +D++ S           +L+       +L+ A               +
Sbjct: 1444 STKYSPRKPIPAKDVRVSSLVRRFEELSLQLQEKQKRDEELIKARRKRALPVVPSKPVVE 1503

Query: 961  FIHEEGQKLHIHLADDNFVVSDYEGE--LSSIVACVLALLKDQPFQTDSHNEDS------ 1020
              ++  +      ++D   ++D +      S  + V ++ K++  +  S NED+      
Sbjct: 1504 VFNDLNEAFDDENSEDENGINDTKENRATESNFSGVDSMSKER--ENVSSNEDNSPEAFE 1563

Query: 1021 -------KGEGGGALQPALSSRSL-----NKVPSNGSSDSESSI---------------- 1080
                   K E G   Q  L   S      +K+P++G    ++S+                
Sbjct: 1564 DIFGILFKNESGLEEQQNLEPSSQMDKEGSKLPTSGPLADKTSVYRILSAFWNEWNSLNP 1623

Query: 1081 -------SSEEYRFSSFD---RLNLLDSLVSETFKRSDH-----------------EGVI 1140
                      E+ FS  +   R +   SL+S T    D+                 +G+ 
Sbjct: 1624 PPFEFPLQPTEHMFSDSNVIIREDEPSSLISFTLSSPDYLSKMVEIEDSMDEALTNQGLQ 1683

Query: 1141 KS--------------------LAKGKYLVNCP--YINQFRDLRSRCCPSELHFIASLSR 1200
             S                      +G   ++C   +  QF  LR R C  E  F+ SL+R
Sbjct: 1684 GSTQFKIENLMLKPTGTHLKYQFEEGSARLSCKVFFAEQFSALR-RACGCEETFVTSLAR 1743

Query: 1201 CINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTCLA 1260
            C  W + GGKS S F KT D ++I+K + R E DS + FAP YF YI++ F     T L 
Sbjct: 1744 CSLWESSGGKSGSAFLKTFDKKYILKVLSRLESDSLLNFAPAYFDYISKVFFHELPTALT 1803

BLAST of MELO3C013178 vs. ExPASy TrEMBL
Match: A0A5D3DIR8 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G001430 PE=4 SV=1)

HSP 1 Score: 2559.3 bits (6632), Expect = 0.0e+00
Identity = 1293/1296 (99.77%), Postives = 1294/1296 (99.85%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 60
            MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT
Sbjct: 308  MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 367

Query: 61   ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 120
            ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD
Sbjct: 368  ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 427

Query: 121  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 180
            AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV
Sbjct: 428  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 487

Query: 181  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP 240
            VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP
Sbjct: 488  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP 547

Query: 241  TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGESFLLT 300
            TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGESFLLT
Sbjct: 548  TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGESFLLT 607

Query: 301  SPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVSHQST 360
            SPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVSHQST
Sbjct: 608  SPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVSHQST 667

Query: 361  LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV 420
            LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV
Sbjct: 668  LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV 727

Query: 421  PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW 480
            PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW
Sbjct: 728  PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW 787

Query: 481  MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF 540
            MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF
Sbjct: 788  MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF 847

Query: 541  LYFFG--LGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSE 600
            LYFFG  LGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSE
Sbjct: 848  LYFFGYVLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSE 907

Query: 601  IAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFH 660
            IAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFH
Sbjct: 908  IAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFH 967

Query: 661  KFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEE 720
            KFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEE
Sbjct: 968  KFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEE 1027

Query: 721  SESIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLS 780
            SESIAENDD+KIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLS
Sbjct: 1028 SESIAENDDSKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLS 1087

Query: 781  PKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSE 840
            PKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSE
Sbjct: 1088 PKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSE 1147

Query: 841  IRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGEL 900
            IRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGEL
Sbjct: 1148 IRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGEL 1207

Query: 901  SSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSE 960
            SSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSE
Sbjct: 1208 SSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSE 1267

Query: 961  EYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSEL 1020
            EYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSEL
Sbjct: 1268 EYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSEL 1327

Query: 1021 HFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFD 1080
            HFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFD
Sbjct: 1328 HFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFD 1387

Query: 1081 MGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPA 1140
            MGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPA
Sbjct: 1388 MGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPA 1447

Query: 1141 TNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAE 1200
            TNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAE
Sbjct: 1448 TNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAE 1507

Query: 1201 KKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVP 1260
            KKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVP
Sbjct: 1508 KKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVP 1567

Query: 1261 DHWCMQQNLPGPWDLCGTADCASSNKTNANENVLSS 1295
            DHWCMQQNLPGPWDLCGTADCASSNKTNANENVLSS
Sbjct: 1568 DHWCMQQNLPGPWDLCGTADCASSNKTNANENVLSS 1603

BLAST of MELO3C013178 vs. ExPASy TrEMBL
Match: A0A1S3BI51 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo OX=3656 GN=LOC103490153 PE=4 SV=1)

HSP 1 Score: 2478.0 bits (6421), Expect = 0.0e+00
Identity = 1257/1294 (97.14%), Postives = 1257/1294 (97.14%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 60
            MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT
Sbjct: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 60

Query: 61   ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 120
            ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD
Sbjct: 61   ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 120

Query: 121  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 180
            AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV
Sbjct: 121  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 180

Query: 181  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP 240
            VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP
Sbjct: 181  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP 240

Query: 241  TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGESFLLT 300
            TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMG      
Sbjct: 241  TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMG------ 300

Query: 301  SPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVSHQST 360
                                           AVYQFDEEVRGSSDGENSVHEQSVSHQST
Sbjct: 301  -------------------------------AVYQFDEEVRGSSDGENSVHEQSVSHQST 360

Query: 361  LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV 420
            LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV
Sbjct: 361  LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV 420

Query: 421  PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW 480
            PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW
Sbjct: 421  PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW 480

Query: 481  MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF 540
            MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF
Sbjct: 481  MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF 540

Query: 541  LYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSEIA 600
            LYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSEIA
Sbjct: 541  LYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSEIA 600

Query: 601  HSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFHKF 660
            HSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFHKF
Sbjct: 601  HSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFHKF 660

Query: 661  LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEESE 720
            LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEESE
Sbjct: 661  LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEESE 720

Query: 721  SIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLSPK 780
            SIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLSPK
Sbjct: 721  SIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLSPK 780

Query: 781  KLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSEIR 840
            KLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSEIR
Sbjct: 781  KLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSEIR 840

Query: 841  QMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGELSS 900
            QMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGELSS
Sbjct: 841  QMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGELSS 900

Query: 901  IVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSEEY 960
            IVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSEEY
Sbjct: 901  IVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSEEY 960

Query: 961  RFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHF 1020
            RFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHF
Sbjct: 961  RFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHF 1020

Query: 1021 IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMG 1080
            IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMG
Sbjct: 1021 IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMG 1080

Query: 1081 NQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATN 1140
            NQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATN
Sbjct: 1081 NQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATN 1140

Query: 1141 NFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAEKK 1200
            NFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAEKK
Sbjct: 1141 NFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAEKK 1200

Query: 1201 ELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDH 1260
            ELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDH
Sbjct: 1201 ELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDH 1257

Query: 1261 WCMQQNLPGPWDLCGTADCASSNKTNANENVLSS 1295
            WCMQQNLPGPWDLCGTADCASSNKTNANENVLSS
Sbjct: 1261 WCMQQNLPGPWDLCGTADCASSNKTNANENVLSS 1257

BLAST of MELO3C013178 vs. ExPASy TrEMBL
Match: A0A0A0K755 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_7G206930 PE=4 SV=1)

HSP 1 Score: 2362.0 bits (6120), Expect = 0.0e+00
Identity = 1199/1294 (92.66%), Postives = 1234/1294 (95.36%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 60
            MVFKKHAAHKHMPTHCKNPKL+LIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT
Sbjct: 189  MVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 248

Query: 61   ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 120
            ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD
Sbjct: 249  ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 308

Query: 121  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 180
            AVYFQKIVEEHAGVLEGGKM TKTLMFIEGCPTRLGCTILLKGAHSDELKRVK+VVQCAV
Sbjct: 309  AVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKIVVQCAV 368

Query: 181  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQP 240
            VMAF LILETSFIVDQ+AMFATIPFGGVP+ ASSD QSPLEPRSP VG+AESASD+ EQP
Sbjct: 369  VMAFHLILETSFIVDQRAMFATIPFGGVPATASSDRQSPLEPRSPNVGKAESASDKSEQP 428

Query: 241  TNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGESFLLT 300
            TNI IS GSHEE S  SN+E  EKLI+SSEPEPYNPAIFSGFSSISDSLKR MGESFL +
Sbjct: 429  TNICISRGSHEETSTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESFLSS 488

Query: 301  SPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVSHQST 360
            SPY SLSSYFGH SDLSGLVAKS SIPST QAVYQFD EV+GSSD ENSVHE SVSHQST
Sbjct: 489  SPYQSLSSYFGHGSDLSGLVAKSGSIPSTPQAVYQFDVEVKGSSDEENSVHESSVSHQST 548

Query: 361  LEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDV 420
            LEGLGFHETA N + DTMQKK  LDSQSILVLMSSRNALKG +CEQSHFSHIVFYK+FDV
Sbjct: 549  LEGLGFHETALNYSVDTMQKKMSLDSQSILVLMSSRNALKGAICEQSHFSHIVFYKHFDV 608

Query: 421  PLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETEGKLW 480
            PLGKFLQENLLNQNNLCTVCGELPEAH Y++AHYRKQLSIQVKQLPGDKVLPGETEGKLW
Sbjct: 609  PLGKFLQENLLNQNNLCTVCGELPEAHIYFFAHYRKQLSIQVKQLPGDKVLPGETEGKLW 668

Query: 481  MWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDF 540
            MWSRCSKCKS GGPSKSTKRV ISTAARGLSFGKFLELCFSDDTL SKS VCGHSLFGDF
Sbjct: 669  MWSRCSKCKSTGGPSKSTKRVPISTAARGLSFGKFLELCFSDDTLLSKSPVCGHSLFGDF 728

Query: 541  LYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLFSEIA 600
            LYFFGLGNMVAM RYSEVAIYTVSMPPQKLEFNSSMRQGH I+E ENVYTKG+LLF+EIA
Sbjct: 729  LYFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHFIRETENVYTKGVLLFTEIA 788

Query: 601  HSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLAFHKF 660
            HSLKKIISER SSNVNHPQGF NDFSFVEEMLNEERSEFE+ IQNSLTKKGN D     F
Sbjct: 789  HSLKKIISERKSSNVNHPQGFPNDFSFVEEMLNEERSEFEINIQNSLTKKGNPD-----F 848

Query: 661  LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGPEESE 720
            LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSG+SE VEPEP++LKMNSNI+VGPEE E
Sbjct: 849  LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPEELE 908

Query: 721  SIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDDLSPK 780
            SIAEND+TK+QQDIS+DENVLPLKEI VEGSDGESGGDELNLPSAIEVTEI IIDD SPK
Sbjct: 909  SIAENDETKVQQDISLDENVLPLKEITVEGSDGESGGDELNLPSAIEVTEIPIIDDPSPK 968

Query: 781  KLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPFSEIR 840
            KLSRQGTLSNGFD HHSDYEDSQVGRVLSSGDLH+DRTIPISIGDSTLSKLFCTPFSEIR
Sbjct: 969  KLSRQGTLSNGFDYHHSDYEDSQVGRVLSSGDLHIDRTIPISIGDSTLSKLFCTPFSEIR 1028

Query: 841  QMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEGELSS 900
            QMHLRDIQRSYFPELKSISSYTPKLL AASDFIHEEGQKLHIHLA+DNFVVSDYEGELSS
Sbjct: 1029 QMHLRDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHLANDNFVVSDYEGELSS 1088

Query: 901  IVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSISSEEY 960
            IVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDS+SSISSEEY
Sbjct: 1089 IVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISSEEY 1148

Query: 961  RFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHF 1020
            RFSSFDRLNLLDSLVSETFKRSD+EGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHF
Sbjct: 1149 RFSSFDRLNLLDSLVSETFKRSDYEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHF 1208

Query: 1021 IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMG 1080
            IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMG
Sbjct: 1209 IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMG 1268

Query: 1081 NQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATN 1140
            NQTCLAKV+GIYQVTVRE KSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATN
Sbjct: 1269 NQTCLAKVVGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATN 1328

Query: 1141 NFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAEKK 1200
            NFGEVLLDQNFVNDMNSSPLYVSNRA RRLQRAIWNDT FLNSINVMDYSLLVGVDAEKK
Sbjct: 1329 NFGEVLLDQNFVNDMNSSPLYVSNRAMRRLQRAIWNDTAFLNSINVMDYSLLVGVDAEKK 1388

Query: 1201 ELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDH 1260
            ELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTV+SPKEYKRRFRKFMSAHFLSVPDH
Sbjct: 1389 ELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSVPDH 1448

Query: 1261 WCMQQNLPGPWDLCGTADCASSNKTNANENVLSS 1295
            WCMQQNLPGPWDL  TAD ASS++TNA+EN  SS
Sbjct: 1449 WCMQQNLPGPWDLFDTADPASSHRTNADENDASS 1477

BLAST of MELO3C013178 vs. ExPASy TrEMBL
Match: A0A6J1F0I5 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111438379 PE=4 SV=1)

HSP 1 Score: 2006.9 bits (5198), Expect = 0.0e+00
Identity = 1032/1272 (81.13%), Postives = 1118/1272 (87.89%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 60
            MVFKKHAAHKHM T+ KNP+L+LIQG LGEAPIS LSSFNSMDQENDFTN+VIEMIE CT
Sbjct: 300  MVFKKHAAHKHMVTNWKNPRLILIQGRLGEAPISGLSSFNSMDQENDFTNYVIEMIETCT 359

Query: 61   ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 120
            ANVI+VEKTA+RVIQEAILK+ +TLVLDMKLHRL+RIALCTGSPILTSETL SQK RQCD
Sbjct: 360  ANVIIVEKTASRVIQEAILKRCITLVLDMKLHRLERIALCTGSPILTSETLASQKARQCD 419

Query: 121  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 180
            AVYFQK+V EH+G LEGGK STKTLMFIEGCP RLGCTILLKGA S ELKRVKLVVQ AV
Sbjct: 420  AVYFQKVV-EHSGGLEGGKRSTKTLMFIEGCPARLGCTILLKGARS-ELKRVKLVVQRAV 479

Query: 181  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQ- 240
            VMAF LILETSF+VDQ+AMFATIPFGGV ++ S DPQSPLEP S  + Q E A+D+ E  
Sbjct: 480  VMAFHLILETSFLVDQRAMFATIPFGGVSTMVSPDPQSPLEPCSSNILQVEKANDKSEPC 539

Query: 241  ---PTNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGES 300
                T+I+ISSG HEE+SN SN++ VEK I+ SE EPYNPAI SGFSSISDS K+ MGES
Sbjct: 540  VSLLTDIYISSGPHEESSNGSNLKPVEKPIIPSELEPYNPAILSGFSSISDSSKKVMGES 599

Query: 301  FLLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVS 360
            FLL+S + SLSSYFGHE D  GL  KSDSIPST QA  +FD +VRGSSD ENS HEQS S
Sbjct: 600  FLLSSTHHSLSSYFGHERDWIGLAPKSDSIPSTPQAANRFDVKVRGSSDEENSGHEQSAS 659

Query: 361  HQSTLEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYK 420
            HQS LEGLGFHE APN + +TMQKK  LDSQSILVLMSSRNALKGTMCEQSHF HIVFY+
Sbjct: 660  HQSALEGLGFHENAPNCSEETMQKKSSLDSQSILVLMSSRNALKGTMCEQSHFLHIVFYR 719

Query: 421  NFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETE 480
            NFDVPLGKFLQENLLNQ N CTVC ELPEAHFYY+AHY KQLSIQV+QLPG K+LPG+TE
Sbjct: 720  NFDVPLGKFLQENLLNQTNFCTVCSELPEAHFYYFAHYGKQLSIQVQQLPGYKLLPGQTE 779

Query: 481  GKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSL 540
            GKLWMWSRCSKCKS G PS STK VLISTAAR LSFGKFLELCFSD+TLPSKSS CGHSL
Sbjct: 780  GKLWMWSRCSKCKSKGEPSTSTKMVLISTAARSLSFGKFLELCFSDETLPSKSSGCGHSL 839

Query: 541  FGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLF 600
            FGDFLYFFGLGN VAMFRYS  AIYTVSMPPQKL+FNSSMRQGHLI+E ENV  KG LLF
Sbjct: 840  FGDFLYFFGLGNRVAMFRYSAAAIYTVSMPPQKLDFNSSMRQGHLIRETENVKKKGKLLF 899

Query: 601  SEIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLA 660
            +EIAHSLKKI SE  SSNV  PQ FLNDFS +EEMLN+ERSEFEVKIQNS        LA
Sbjct: 900  TEIAHSLKKISSECRSSNVK-PQCFLNDFSSIEEMLNQERSEFEVKIQNS--------LA 959

Query: 661  FHKFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGP 720
            FHK+L+LN+LLW+LLLESCIWDRRLQSL SLG+T+  GTSENVEPE V + MNSNI+   
Sbjct: 960  FHKYLSLNQLLWELLLESCIWDRRLQSLTSLGLTSTPGTSENVEPESVTMNMNSNIDARH 1019

Query: 721  EESESIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDD 780
            EESESIA NDDT++QQDIS DENVLP+KEIAVEG DGESG DE + PS  EVTEI+I+DD
Sbjct: 1020 EESESIAANDDTEVQQDISWDENVLPMKEIAVEGLDGESGCDENHSPSVTEVTEIRIMDD 1079

Query: 781  LSPKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPF 840
            LSPK LSRQG+LSNGF+ HH D EDSQV RV SSGD+HVDRTIP+   DS+L KL   P 
Sbjct: 1080 LSPKHLSRQGSLSNGFNQHHLDDEDSQV-RVASSGDMHVDRTIPVLTADSSLGKLLWAPC 1139

Query: 841  SEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEG 900
             EIRQMH+RDIQRS+FPELKS SSYTPKL+ AAS F +EEGQKLHI LA DNF+VSDYEG
Sbjct: 1140 VEIRQMHIRDIQRSFFPELKSFSSYTPKLIPAASAFTNEEGQKLHIRLAKDNFIVSDYEG 1199

Query: 901  ELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSIS 960
            ELSSIVACVLALL+D   QTDSHNEDSKGEGGG  QP LSS+SLNKVPSNGSSDS+SS +
Sbjct: 1200 ELSSIVACVLALLEDLSLQTDSHNEDSKGEGGGVSQPNLSSQSLNKVPSNGSSDSDSSAA 1259

Query: 961  SEEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPS 1020
            S+ Y+FS+F+RLNLLDSLV ETFKR+DHEGVIKSLA GKY+VNCPY NQFRDLRS+CCPS
Sbjct: 1260 SDAYQFSNFERLNLLDSLVPETFKRTDHEGVIKSLANGKYVVNCPYFNQFRDLRSQCCPS 1319

Query: 1021 ELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINES 1080
            ELH+IASLSRCI WNAKGGKS SFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYI +S
Sbjct: 1320 ELHYIASLSRCIKWNAKGGKSNSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYIKQS 1379

Query: 1081 FDMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFN 1140
            FDMGNQTCLAKVLGIYQV VRE KSGKEMRHD+MVMENLSFGRNIIRQYDLKGALHAR+N
Sbjct: 1380 FDMGNQTCLAKVLGIYQVAVREPKSGKEMRHDVMVMENLSFGRNIIRQYDLKGALHARYN 1439

Query: 1141 PATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVD 1200
            PA NNFGEVLLDQNFV DMNSSP+YVSNRAKRR+QRAIWNDT FLNSINVMDYSLLVGVD
Sbjct: 1440 PAANNFGEVLLDQNFVKDMNSSPIYVSNRAKRRMQRAIWNDTAFLNSINVMDYSLLVGVD 1499

Query: 1201 AEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLS 1260
            AEKKE VCGIIDYLRQYTWDKQLETWVKSSL+PKNVLPTV+SPKEYKRRFRKFMSAHFLS
Sbjct: 1500 AEKKEFVCGIIDYLRQYTWDKQLETWVKSSLVPKNVLPTVISPKEYKRRFRKFMSAHFLS 1559

Query: 1261 VPDHWCMQQNLP 1269
            VPDHWC+QQN P
Sbjct: 1560 VPDHWCLQQNFP 1559

BLAST of MELO3C013178 vs. ExPASy TrEMBL
Match: A0A6J1EVX9 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111438379 PE=4 SV=1)

HSP 1 Score: 2006.9 bits (5198), Expect = 0.0e+00
Identity = 1032/1272 (81.13%), Postives = 1118/1272 (87.89%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCT 60
            MVFKKHAAHKHM T+ KNP+L+LIQG LGEAPIS LSSFNSMDQENDFTN+VIEMIE CT
Sbjct: 1    MVFKKHAAHKHMVTNWKNPRLILIQGRLGEAPISGLSSFNSMDQENDFTNYVIEMIETCT 60

Query: 61   ANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCD 120
            ANVI+VEKTA+RVIQEAILK+ +TLVLDMKLHRL+RIALCTGSPILTSETL SQK RQCD
Sbjct: 61   ANVIIVEKTASRVIQEAILKRCITLVLDMKLHRLERIALCTGSPILTSETLASQKARQCD 120

Query: 121  AVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQCAV 180
            AVYFQK+V EH+G LEGGK STKTLMFIEGCP RLGCTILLKGA S ELKRVKLVVQ AV
Sbjct: 121  AVYFQKVV-EHSGGLEGGKRSTKTLMFIEGCPARLGCTILLKGARS-ELKRVKLVVQRAV 180

Query: 181  VMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQIEQ- 240
            VMAF LILETSF+VDQ+AMFATIPFGGV ++ S DPQSPLEP S  + Q E A+D+ E  
Sbjct: 181  VMAFHLILETSFLVDQRAMFATIPFGGVSTMVSPDPQSPLEPCSSNILQVEKANDKSEPC 240

Query: 241  ---PTNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAMGES 300
                T+I+ISSG HEE+SN SN++ VEK I+ SE EPYNPAI SGFSSISDS K+ MGES
Sbjct: 241  VSLLTDIYISSGPHEESSNGSNLKPVEKPIIPSELEPYNPAILSGFSSISDSSKKVMGES 300

Query: 301  FLLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQSVS 360
            FLL+S + SLSSYFGHE D  GL  KSDSIPST QA  +FD +VRGSSD ENS HEQS S
Sbjct: 301  FLLSSTHHSLSSYFGHERDWIGLAPKSDSIPSTPQAANRFDVKVRGSSDEENSGHEQSAS 360

Query: 361  HQSTLEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYK 420
            HQS LEGLGFHE APN + +TMQKK  LDSQSILVLMSSRNALKGTMCEQSHF HIVFY+
Sbjct: 361  HQSALEGLGFHENAPNCSEETMQKKSSLDSQSILVLMSSRNALKGTMCEQSHFLHIVFYR 420

Query: 421  NFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPGETE 480
            NFDVPLGKFLQENLLNQ N CTVC ELPEAHFYY+AHY KQLSIQV+QLPG K+LPG+TE
Sbjct: 421  NFDVPLGKFLQENLLNQTNFCTVCSELPEAHFYYFAHYGKQLSIQVQQLPGYKLLPGQTE 480

Query: 481  GKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSL 540
            GKLWMWSRCSKCKS G PS STK VLISTAAR LSFGKFLELCFSD+TLPSKSS CGHSL
Sbjct: 481  GKLWMWSRCSKCKSKGEPSTSTKMVLISTAARSLSFGKFLELCFSDETLPSKSSGCGHSL 540

Query: 541  FGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGMLLF 600
            FGDFLYFFGLGN VAMFRYS  AIYTVSMPPQKL+FNSSMRQGHLI+E ENV  KG LLF
Sbjct: 541  FGDFLYFFGLGNRVAMFRYSAAAIYTVSMPPQKLDFNSSMRQGHLIRETENVKKKGKLLF 600

Query: 601  SEIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKGNLDLA 660
            +EIAHSLKKI SE  SSNV  PQ FLNDFS +EEMLN+ERSEFEVKIQNS        LA
Sbjct: 601  TEIAHSLKKISSECRSSNVK-PQCFLNDFSSIEEMLNQERSEFEVKIQNS--------LA 660

Query: 661  FHKFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSNINVGP 720
            FHK+L+LN+LLW+LLLESCIWDRRLQSL SLG+T+  GTSENVEPE V + MNSNI+   
Sbjct: 661  FHKYLSLNQLLWELLLESCIWDRRLQSLTSLGLTSTPGTSENVEPESVTMNMNSNIDARH 720

Query: 721  EESESIAENDDTKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEIQIIDD 780
            EESESIA NDDT++QQDIS DENVLP+KEIAVEG DGESG DE + PS  EVTEI+I+DD
Sbjct: 721  EESESIAANDDTEVQQDISWDENVLPMKEIAVEGLDGESGCDENHSPSVTEVTEIRIMDD 780

Query: 781  LSPKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKLFCTPF 840
            LSPK LSRQG+LSNGF+ HH D EDSQV RV SSGD+HVDRTIP+   DS+L KL   P 
Sbjct: 781  LSPKHLSRQGSLSNGFNQHHLDDEDSQV-RVASSGDMHVDRTIPVLTADSSLGKLLWAPC 840

Query: 841  SEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVVSDYEG 900
             EIRQMH+RDIQRS+FPELKS SSYTPKL+ AAS F +EEGQKLHI LA DNF+VSDYEG
Sbjct: 841  VEIRQMHIRDIQRSFFPELKSFSSYTPKLIPAASAFTNEEGQKLHIRLAKDNFIVSDYEG 900

Query: 901  ELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSESSIS 960
            ELSSIVACVLALL+D   QTDSHNEDSKGEGGG  QP LSS+SLNKVPSNGSSDS+SS +
Sbjct: 901  ELSSIVACVLALLEDLSLQTDSHNEDSKGEGGGVSQPNLSSQSLNKVPSNGSSDSDSSAA 960

Query: 961  SEEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPS 1020
            S+ Y+FS+F+RLNLLDSLV ETFKR+DHEGVIKSLA GKY+VNCPY NQFRDLRS+CCPS
Sbjct: 961  SDAYQFSNFERLNLLDSLVPETFKRTDHEGVIKSLANGKYVVNCPYFNQFRDLRSQCCPS 1020

Query: 1021 ELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINES 1080
            ELH+IASLSRCI WNAKGGKS SFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYI +S
Sbjct: 1021 ELHYIASLSRCIKWNAKGGKSNSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYIKQS 1080

Query: 1081 FDMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFN 1140
            FDMGNQTCLAKVLGIYQV VRE KSGKEMRHD+MVMENLSFGRNIIRQYDLKGALHAR+N
Sbjct: 1081 FDMGNQTCLAKVLGIYQVAVREPKSGKEMRHDVMVMENLSFGRNIIRQYDLKGALHARYN 1140

Query: 1141 PATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVD 1200
            PA NNFGEVLLDQNFV DMNSSP+YVSNRAKRR+QRAIWNDT FLNSINVMDYSLLVGVD
Sbjct: 1141 PAANNFGEVLLDQNFVKDMNSSPIYVSNRAKRRMQRAIWNDTAFLNSINVMDYSLLVGVD 1200

Query: 1201 AEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLS 1260
            AEKKE VCGIIDYLRQYTWDKQLETWVKSSL+PKNVLPTV+SPKEYKRRFRKFMSAHFLS
Sbjct: 1201 AEKKEFVCGIIDYLRQYTWDKQLETWVKSSLVPKNVLPTVISPKEYKRRFRKFMSAHFLS 1260

Query: 1261 VPDHWCMQQNLP 1269
            VPDHWC+QQN P
Sbjct: 1261 VPDHWCLQQNFP 1260

BLAST of MELO3C013178 vs. TAIR 10
Match: AT1G34260.1 (FORMS APLOID AND BINUCLEATE CELLS 1A )

HSP 1 Score: 1023.5 bits (2645), Expect = 1.5e-298
Identity = 612/1294 (47.30%), Postives = 797/1294 (61.59%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQEND----FTNHVIEMI 60
            +VFKKHAA KHM T  ++P+++L++G+LG  PIS  SS  S++Q+N+    +   V+++I
Sbjct: 230  LVFKKHAALKHMATKYEHPRIMLVEGVLGH-PISGFSSLQSVNQDNEYLLKYVKPVVDII 289

Query: 61   EGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKT 120
            E    +V+LVEK+ +R IQ+ IL KG+TLV DMKLHRLQRI+ C GSPIL+ ++L SQK 
Sbjct: 290  EASKPDVMLVEKSVSRDIQKTILDKGVTLVFDMKLHRLQRISRCIGSPILSVDSLSSQKL 349

Query: 121  RQCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVV 180
            + CD+   +KIVEEH    E  K  TKTLMF+EGCPTRLGCTILLKG HS+ LK+VK VV
Sbjct: 350  KHCDSFRIEKIVEEHNAAGESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVKEVV 409

Query: 181  QCAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQ 240
            Q + ++A+ L+LE SF+ D+  MF+TI      S         +E  SP     ES S+ 
Sbjct: 410  QYSFILAYHLMLEASFLADRHTMFSTIFAKEATSCVVE-----IENFSPSPSPRESPSEA 469

Query: 241  IEQPTNIHISSGSHEEA---SNESNVETVEKLIVSSE----PEPYNPAIFSGFSSISDSL 300
            ++ P    +S+G  E+    + E++ E V       +     EPYNP IF+GFSS+S  L
Sbjct: 470  VDIP----VSNGFDEQTIQINGEADGEKVGTWESDGDHVFSHEPYNPVIFTGFSSLSARL 529

Query: 301  KRAMGESFLLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENS 360
                       S YL             G V   +S+P ++         +    + E  
Sbjct: 530  -----------SKYL-------------GFVQNPESVPVSVDTDVSTTSNLDSIRESEED 589

Query: 361  VHEQSVSHQSTLEGLGFHETAPNDTGDTMQK-----KPPLDSQSILVLMSSRNALKGTMC 420
              E++   Q  L        + +D GD   +     +  L+SQSILVL+S RNAL+G MC
Sbjct: 590  TAEKNEDKQPLLLDPELPVNSSSDDGDNKSQTENDIESTLESQSILVLVSKRNALRGIMC 649

Query: 421  EQSHFSHIVFYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQ 480
            +Q HFSHI FYK+FDVPL KFL++ + NQ NLC  C E PEAH YYYAH  KQL+IQ+K+
Sbjct: 650  DQRHFSHIKFYKHFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLTIQIKR 709

Query: 481  LPGDKVLPGETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDT 540
            +P  K L GE +GK+WMWSRC KCK+     KSTKRVLISTAAR LSFGKFLEL FS  T
Sbjct: 710  IPVAKGLAGEAKGKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELSFSQQT 769

Query: 541  LPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKE 600
              ++SS CGHS   DFL+FFGLG+MVAM  YS+VA YTVS+PP KLE +  ++ G L KE
Sbjct: 770  FLNRSSSCGHSFDSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAGWLEKE 829

Query: 601  IENVYTKGMLLFSEIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQ 660
             + V+TKG+ LF + A  LK++ S+  +S++ + Q      S +EE+L  ER  FE  I+
Sbjct: 830  FQTVFTKGISLFEDAAGFLKRLRSQFTNSDLRY-QRARKLLSNIEELLKHERCIFEENIK 889

Query: 661  NSLTKKGNLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPV 720
            NS  K   +D   H+ L LNR+ W+LLL++ IW+ RLQSL           S+ + P   
Sbjct: 890  NSFDKAKTIDDVSHRLLRLNRMRWELLLQALIWNYRLQSLV---------LSDRLLPSSD 949

Query: 721  MLKMNSNINVGPEESESIAENDDTKIQQDIS---VDENVLPLKEIAVEGSDGESGGDELN 780
              K+         E+      +D K+    S   +D  ++  K+I + G+      D++ 
Sbjct: 950  ETKIYEQGLKTVSEAGMTRYENDNKVSDSGSNGGIDTPLVEHKDIPIAGA-SVGDNDQMA 1009

Query: 781  LPSAIEVTEIQIIDDLSPKKLSRQGTLSNGFDCH-----HSDYEDSQVGRVLSSGDLHVD 840
                 E  E Q +   SP   S    ++N FD H     HS         +  +G+  +D
Sbjct: 1010 ESYVPEDNESQTLCSSSPDTTS---PINNHFDTHLAVNVHSTNGQEADKSIPVTGE-SLD 1069

Query: 841  RTIPISIGDSTL--SKLFCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIH 900
              +  S G   L   + F  PF E+R   + DI++ Y  + + ++++T + L   +  I 
Sbjct: 1070 DEVSTSNGPHILGWDEWFWLPFEELRSKRIVDIEKEYLLKFEYVNNFTQENLQTVNQIIT 1129

Query: 901  EEGQKLHIHLADDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPA 960
            EE  +L I L DD+F+VSDYE ELSS++AC LA L         +NE+SK      +  +
Sbjct: 1130 EESSRLRISLRDDDFIVSDYEDELSSLIACALAHL---------NNEESKKPLSRCIHGS 1189

Query: 961  LSSRSLNKVPSNGSSDSESSISSEEYRFSSFDRLNLL-DSLVSETFKRSDHEGVIKSLAK 1020
            L     N   S  +    S  SSE     S +RL  L    V  TF      G +KS+ K
Sbjct: 1190 LQGFLDNNQDSKQTDRDVSRFSSE-----STNRLETLPPPEVLVTF------GSVKSVGK 1249

Query: 1021 GKYLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKE 1080
             KY +   Y + FRDLR RCC SEL +IASLSRC  W+AKGGKSKS FAKTLDDRFI+KE
Sbjct: 1250 PKYSIVSLYADDFRDLRKRCCSSELDYIASLSRCKPWDAKGGKSKSVFAKTLDDRFIVKE 1309

Query: 1081 IKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTVREQK-SGKEMRHDLMVM 1140
            IK+TEY+SF+ FA EYFKY+ +S+D+GNQTCLAKVLGI+QVTVR+ K  GKE+RHDLMVM
Sbjct: 1310 IKKTEYESFVTFATEYFKYMKDSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIRHDLMVM 1369

Query: 1141 ENLSFGRNIIRQYDLKGALHARFN-PATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQ 1200
            ENLSF R + RQYDLKGALHARF   + N   +VLLDQNFVNDMN SPLYVS  +K+ LQ
Sbjct: 1370 ENLSFSRKVTRQYDLKGALHARFTATSANGEDDVLLDQNFVNDMNKSPLYVSKTSKQNLQ 1429

Query: 1201 RAIWNDTTFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSL-IPK 1260
            RA++NDT+FL SINVMDYSLLVGVD E  ELVCGIIDYLRQYTWDKQLETWVKSSL +PK
Sbjct: 1430 RAVYNDTSFLTSINVMDYSLLVGVDDENHELVCGIIDYLRQYTWDKQLETWVKSSLVVPK 1453

Query: 1261 NVLPTVVSPKEYKRRFRKFMSAHFLSVPDHWCMQ 1265
            NV PTV+SP +YK RFRKFM  HFL VPD WC Q
Sbjct: 1490 NVQPTVISPIDYKTRFRKFMKTHFLCVPDQWCDQ 1453

BLAST of MELO3C013178 vs. TAIR 10
Match: AT3G14270.1 (phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 691.0 bits (1782), Expect = 1.8e-198
Identity = 485/1338 (36.25%), Postives = 710/1338 (53.06%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPIS-RLSSFNS-MDQENDFTNHVIEMIEG 60
            +V KK+  ++ M T  +  +L+++ G L    +S +LSSF++ + QE D     +  I  
Sbjct: 459  VVCKKNVVNRRMSTKIEKARLLILGGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHA 518

Query: 61   CTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTS-ETLMSQKTR 120
               N++LVEK+ +R  QE +L K ++LVL++K   L RIA CTG+ I+ S + L SQK  
Sbjct: 519  ERPNILLVEKSVSRFAQEYLLAKDISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLG 578

Query: 121  QCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQ 180
             C+     +  EEH    + GK   KTLM+ E CP  LG TILL+GA+ DELK+VK VVQ
Sbjct: 579  YCENFRVDRYPEEHGSTGQVGKKVVKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQ 638

Query: 181  CAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQI 240
              V  A+ L LETSF+ D+ A    +P     ++A  D  + +E RS       + S   
Sbjct: 639  YGVFAAYHLALETSFLADEGAS-PELPLNSPITVALPDKSTSIE-RSISTVPGFTVSTYE 698

Query: 241  EQPTNIHISSGSHEEASNESN-VETVEKLIVSSE--PEPYNPAIFSGFSSISDSLKRAMG 300
            + PT +  +      +   S  + T   L +  +  P PY     SG+ +      R + 
Sbjct: 699  KSPTMLSCAEPQRANSVPVSELLSTTTNLSIQKDIPPIPYG----SGWQA------REIN 758

Query: 301  ESFLLTSPYLSLS----SYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGS--SDGEN 360
             SF+ +   +SL+          SDLSG     D+       +   DE    S    G+ 
Sbjct: 759  PSFVFSRHNISLNLPDRVIESRNSDLSGRSVPVDTPADKSNPIVVADETTNNSLHLSGQG 818

Query: 361  SVHEQS-------VSHQSTLEGLGF----HETAPNDTGDTMQKKP--PLDSQSILVLMSS 420
             V + S       V +Q     L      +   P +T    ++ P  P D QSILV +SS
Sbjct: 819  FVRKSSQIGTSIMVENQDNGSELTIAQQQNNEKPKETQSQKEEFPPSPSDHQSILVSLSS 878

Query: 421  RNALKGTMCEQSHFSHIVFYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYR 480
            R+  KGT+CE+SH   I +Y +FD PLG+FL+++L +Q+  C  C    EAH + Y H +
Sbjct: 879  RSVWKGTVCERSHLFRIKYYGSFDKPLGRFLRDHLFDQSYRCRSCEMPSEAHVHCYTHRQ 938

Query: 481  KQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKF 540
              L+I VK+L  D +LPGE EGK+WMW RC +C  L G   +T RV++S AA GLSFGKF
Sbjct: 939  GSLTISVKKLQ-DYLLPGEKEGKIWMWHRCLRCPRLNGFPPATLRVVMSDAAWGLSFGKF 998

Query: 541  LELCFSDDTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSS 600
            LEL FS+    S+ + CGHSL  D L F+G GNMVA FRY+ + +++V +PP  L FN  
Sbjct: 999  LELSFSNHAAASRVACCGHSLHRDCLRFYGFGNMVACFRYATIDVHSVYLPPSILSFNYE 1058

Query: 601  MRQGHLIKEIENVYTKGMLLFSEIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEE 660
              Q  + +E + V  +  LLFSE+ +++ +I            +GF      +EE+L +E
Sbjct: 1059 -NQDWIQRETDEVIERAELLFSEVLNAISQIAE----------KGFRRRIGELEEVLQKE 1118

Query: 661  RSEFEVKIQNSLTKKGNLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGT 720
            ++EFE  +Q  L ++ N        L L R+   LL +S +WD RL + ++L    NS  
Sbjct: 1119 KAEFEENMQKILHREVNEGQPLVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDD 1178

Query: 721  SENVEPE----------PVM------LKMNSNINVGPEESESIAENDDTKIQQDISVDEN 780
            ++  E E          P M      L   S +N+ P+   +        +Q++   + +
Sbjct: 1179 TKREENEKPPLAKSQTLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGSLNNVQKEADTNSD 1238

Query: 781  VLPLKEIAVEGSDGESGGD-ELNLPSAIEVTEIQIIDDLSPKKLS----------RQGTL 840
            +   K+   E S  ++  D    L + ++V   Q    +  K LS          RQ ++
Sbjct: 1239 LYQEKDDGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQIVMKNLSATLDAAWIGERQTSV 1298

Query: 841  ----SNGFDCHHSDYEDSQVGRVLSSGDLHVDR-------TIPISIGDSTLSKLFCTPFS 900
                +N      S   +S     +S G + +D         +   +  +  SK +     
Sbjct: 1299 EIPTNNKVSLPPSTMSNSSTFPPISEGLMPIDLPEQQNEFKVAYPVSPALPSKNYENSED 1358

Query: 901  EIRQMHL------RDIQRSYF---PELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDN 960
             +  + +      R I +++     +L +   ++P  +++  +   + G +L + +  ++
Sbjct: 1359 SVSWLSVPFLNFYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLND 1418

Query: 961  FVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQP-ALSSRSLNKVPSNG 1020
             VV  Y+ E +S++A  L +  +   QT +  E           P  +     +   SNG
Sbjct: 1419 IVVPVYDDEPTSMIAYAL-MSPEYQRQTSAEGESLVSYPSELNIPRPVDDTIFDPSRSNG 1478

Query: 1021 SSDSESSISSEEYRFSSFDRLNLLDSL-VSETFKRSDHEGVIKSLAKGKYLVNCPYINQF 1080
            S D ES +S    R +S     LLD L  ++        G   +L K KY V C Y  +F
Sbjct: 1479 SVD-ESILSISSSRSTS-----LLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRF 1538

Query: 1081 RDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFA 1140
              LR  C PSEL +I SLSRC  W A+GGKS  FFAKTLDDRFIIK++ +TE +SF+KFA
Sbjct: 1539 EALRGICLPSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFA 1598

Query: 1141 PEYFKYINESFDMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYD 1200
            P YFKY++ES    + TCLAK+LGIYQV  ++ KSGKE + D+++MENL FGR + R YD
Sbjct: 1599 PAYFKYLSESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYD 1658

Query: 1201 LKGALHARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINV 1260
            LKG+  AR+NP ++   +VLLDQN +  M +SP++V N+AKR L+RA+WNDT FL   +V
Sbjct: 1659 LKGSSRARYNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDV 1718

Query: 1261 MDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLI---PKNVLPTVVSPKEYK 1262
            MDYSLLVGVD EK ELV GIID+LRQYTWDK LE+WVK + I   PKN  PTV+SPK+YK
Sbjct: 1719 MDYSLLVGVDEEKNELVLGIIDFLRQYTWDKHLESWVKFTGILGGPKNEAPTVISPKQYK 1765

BLAST of MELO3C013178 vs. TAIR 10
Match: AT1G71010.1 (FORMS APLOID AND BINUCLEATE CELLS 1C )

HSP 1 Score: 661.0 bits (1704), Expect = 2.0e-189
Identity = 469/1302 (36.02%), Postives = 699/1302 (53.69%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPIS-RLSSFNS-MDQENDFTNHVIEMIEG 60
            +V  K+  HK M +  KNP+++L+ G L    ++ +L+SFN+ + QEN+    +I  IE 
Sbjct: 403  IVCSKNITHKRMISQYKNPRVMLLAGSLEYQRVAGQLASFNTLLQQENEHMKAIIAKIES 462

Query: 61   CTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTS-ETLMSQKTR 120
               NV+LVEK+A+   Q+ +L+K ++LVL++K   L RIA CTG+ +  S +++ + +  
Sbjct: 463  LRPNVLLVEKSASSYAQQYLLEKEISLVLNVKRSLLDRIARCTGAVLCPSLDSISTARLG 522

Query: 121  QCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQ 180
             C+    ++++E+H    +  +  ++TLM+ EGCP RLGCT++L+G+  +ELK+VK V+Q
Sbjct: 523  HCELFRTERVLEQHEAGNQSNRKPSRTLMYFEGCPRRLGCTVVLRGSCREELKKVKHVIQ 582

Query: 181  CAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESASDQI 240
             AV  A+ L LETSF+ D+ A    I       + ++  +  ++     + Q+ + +D  
Sbjct: 583  YAVFAAYHLSLETSFLADEGASLPKIRLKQPGMVRTASQRRIIDEGISLITQSPTETD-- 642

Query: 241  EQPTNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPA-IFSGFSSISDSLKRAMGES 300
               +   + + +HE+   E      E  +  S  E ++P  IF   S +       +   
Sbjct: 643  ---SQALLETAAHED---EHTAPMPEHEVCESLCEDFDPTQIFPPSSEVETEQSDTLNGD 702

Query: 301  F---LLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQ 360
            F   L+T  Y S      HE  L      S  IP T        EE  G  + EN    Q
Sbjct: 703  FANNLVTRSYSSNQLNDLHEPTL----CLSSEIPET-PTQQPSGEEDNGRGEEEN----Q 762

Query: 361  SVSHQSTLEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIV 420
             V+ Q     L  HE+   D   +         QSILV  SSR  LK ++CE+S    I 
Sbjct: 763  LVNPQD----LPQHESFYEDDVSSEYFSAADSHQSILVSFSSRCVLKESVCERSRLLRIK 822

Query: 421  FYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPG 480
            FY +FD PLG++L+++L ++ + C  C EL +AH   Y+H    L+I V++LP  K LPG
Sbjct: 823  FYGSFDKPLGRYLKDDLFDKTSSCRSCKELVDAHVLCYSHQNGNLTINVRRLPSMK-LPG 882

Query: 481  ETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCG 540
            E +GK+WMW RC +C  + G   +T+RV++S AA GLSFGKFLEL FS+    ++ + CG
Sbjct: 883  EQDGKIWMWHRCLRCAHVDGVPPATRRVVMSDAAWGLSFGKFLELSFSNHATANRVASCG 942

Query: 541  HSLFGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTKGM 600
            HSL  D L F+G GNMVA FRYS + I TV +PP  LEFNS  +Q  +  E   +  K  
Sbjct: 943  HSLQRDCLRFYGFGNMVAFFRYSPINILTVLLPPSMLEFNSHPQQEWIRTEAAELVGKMR 1002

Query: 601  LLFSEIAHSLKKIISERNSSNVNHPQGFLNDFSF----VEEMLNEERSEFEVKIQ----N 660
             +++EI+  L ++  E  SS +   Q    D       + + L +E+ E++  +Q     
Sbjct: 1003 TMYTEISDMLNRM--EEKSSLLEPEQSEACDLHSRIIGLIDQLVKEKDEYDDALQPIFEE 1062

Query: 661  SLTKKGNLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSL------ASLGVTTNSGTSENV 720
            +L  +G+LD+     L LNRL   L++ +  WD +L  L      AS+  T +     N 
Sbjct: 1063 NLQIQGSLDI-----LELNRLRRALMIGAHAWDHQLYLLNSQLKKASVFKTGDDNAPRNP 1122

Query: 721  E---PEPVMLKMNSNINVGPEESESIAE-NDDTKIQQDISVDENVLPLKEIAVEGSDGES 780
            E   P  +  +M    +   E+S + +E N D K  ++I      L       E  D   
Sbjct: 1123 EMHDPPKIDRRMQEGSDERDEQSHTDSEANGDNKDPENIPSPGTSLS------ERIDSAW 1182

Query: 781  GGDELNLPSAIEVTEIQIID--DLSPKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDL 840
             G   NL  A  + E +     + S ++L+R   + + FD      E  Q G   SS  L
Sbjct: 1183 LGSFQNLEKAETIAETEGFSAVNSSLRRLARPIRVQS-FDSAIRFQERIQKGLPPSSLYL 1242

Query: 841  HVDRTIPISIGDSTLSKLFCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFI 900
               R+   S        +   P S + + + + +      +L  I    P  +++AS   
Sbjct: 1243 STLRSFHAS---GEYRNMVRDPVSNVMRTYSQMLPLE-VQKLDLIVGSAPTYISSASQM- 1302

Query: 901  HEEGQKLHI-HLADDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQ 960
              +G ++ I     ++ VV  Y+ + +S+V+  +         +  + E    +G   L 
Sbjct: 1303 -ADGARMLIPQRGLNDIVVPVYDDDPASVVSYAI--------NSKEYKEWIVNKG---LA 1362

Query: 961  PALSSRSLNKVPSNGSSDSE--------SSISSEEYRFSSFDRLNLLDSLVSETFKRSDH 1020
             + SS +LN   S  S+ S           I    Y  S  DR +        T   SD 
Sbjct: 1363 SSSSSSNLNNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQDDRKS-----PHLTISFSDR 1422

Query: 1021 EGVIKSLAKG--KYLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFA 1080
                 +  +G  K+ V C +  QF  LR  CCPSE+ F+ SLSRC  W+A+GGKS  +FA
Sbjct: 1423 ASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRCQRWSAQGGKSNVYFA 1482

Query: 1081 KTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTVREQKSG 1140
            K+LD+RFIIK++ +TE DSF  FAPEYFKY+ ES   G+ TCLAK+LGIYQV+++  K G
Sbjct: 1483 KSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAKILGIYQVSIKHPKGG 1542

Query: 1141 KEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFGEVLLDQNFVNDMNSSPLYV 1200
            KE + DLMVMENL + R I R YDLKG+  +R+NP T+   +VLLD N +  + + P+++
Sbjct: 1543 KETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLLDMNLLETLRTEPIFL 1602

Query: 1201 SNRAKRRLQRAIWNDTTFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETW 1260
             ++AKR L+RAIWNDT FL S++VMDYSLLVG D E+KELV GIID++RQYTWDK LETW
Sbjct: 1603 GSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGIIDFMRQYTWDKHLETW 1646

Query: 1261 VKSSLI---PKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDHW 1262
            VK+S I   PKN  PT+VSPK+YKRRFRK M+ +FL+VP+ W
Sbjct: 1663 VKASGILGGPKNASPTIVSPKQYKRRFRKAMTTYFLTVPEPW 1646

BLAST of MELO3C013178 vs. TAIR 10
Match: AT4G33240.1 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 661.0 bits (1704), Expect = 2.0e-189
Identity = 473/1349 (35.06%), Postives = 694/1349 (51.45%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPIS-RLSSFNS-MDQENDFTNHVIEMIEG 60
            +V KK+ AH+ M +  + P+L+++ G L    IS +LSSF++ + QE D     +  I+ 
Sbjct: 447  VVCKKNVAHRRMTSKIEKPRLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDS 506

Query: 61   CTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTS-ETLMSQKTR 120
               +++LVEK+ +R  QE +L K ++LVL++K   L+RI+ CTG+ I+ S + L S K  
Sbjct: 507  HNPDILLVEKSVSRFAQEYLLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLG 566

Query: 121  QCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQ 180
             CD  + +K VE H    +  K   KTLMF +GCP  LGCTILLKGAH DELK+VK V+Q
Sbjct: 567  YCDLFHVEKFVETHVSPCQVAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQ 626

Query: 181  CAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPL----------------- 240
              V  A+ L LETSF+ D+ A    +P     ++A  D  S +                 
Sbjct: 627  YGVFAAYHLALETSFLADEGASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEK 686

Query: 241  EPRSPKVGQAESASDQIEQPTNIHISSGSHEEASNESN--VETVEKLIVSSEPEPYNPAI 300
             P +   G+   A+  +   T    SS +H +   + N  ++  E+L+ + +     P  
Sbjct: 687  SPTTELRGEPHKANGDL---TGNFTSSKTHFQGKLDGNDRIDPSERLLHNLDTVYCKPP- 746

Query: 301  FSGFSSISDSLKRAMGE---SFLLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQ 360
                +S  D L   +     SF +  P +    +    S LSG      +        Y 
Sbjct: 747  -ETITSKDDGLVPTLESRQLSFHVEEPSVQKDQW----SVLSG------ATEQVTDGGYT 806

Query: 361  FDEEVRGSSDGENSVHEQSVSHQSTLEGL--GFHETAPNDTGDTMQKKPPLDSQSILVLM 420
             D  V G+         Q+ + Q  +E     FH +A              D QSILV +
Sbjct: 807  NDSAVIGN---------QNFNRQEQMESSKGDFHPSAS-------------DHQSILVSL 866

Query: 421  SSRNALKGTMCEQSHFSHIVFYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAH 480
            S+R   KG++CE++H   I +Y +FD PLG+FL++NL +Q+  C  C    EAH + Y H
Sbjct: 867  STRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCTMPAEAHIHCYTH 926

Query: 481  YRKQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFG 540
             +  L+I VK+LP  ++LPG+ EGK+WMW RC KC  + G   +T+R+++S AA GLSFG
Sbjct: 927  RQGSLTISVKKLP--ELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVMSDAAWGLSFG 986

Query: 541  KFLELCFSDDTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFN 600
            KFLEL FS+    S+ + CGHSL  D L F+G G MVA FRY+ + IY V++PP KL FN
Sbjct: 987  KFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAVTLPPAKLYFN 1046

Query: 601  SSMRQGHLIKEIENVYTKGMLLFSEIAHSLKKIISER--NSSNVNHPQGFLNDFSFVEEM 660
                Q  L KE + V  K  +LF+E+  +L +I ++     S  + P         +  +
Sbjct: 1047 YE-NQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIKLSLEELAGL 1106

Query: 661  LNEERSEFEVKIQNSL--TKKGNLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSLASL-- 720
            L + + E++  +Q  L   K G   +     L +N+L   ++ +S  WD  L   A++  
Sbjct: 1107 LEQRKKEYKDSLQQMLNVVKDGQPTI---DILLINKLRRLIIFDSYAWDECLAGAANMVR 1166

Query: 721  -----------------GVTTNSGTSENVEPEPV-------MLKMNSNINVGPEESESIA 780
                              V+    + E V+  P         L  +++      + +S A
Sbjct: 1167 NNYLEAPKNSAPKVMGRNVSLEKLSDEKVKSIPTHVAICNDSLLQDADYETCLNQGKSFA 1226

Query: 781  ENDDT-KIQQDISVDENVLPLKEIAVEGSDGESGGDEL----------NLPSAIEVTEIQ 840
            +      I +D+  D       +  +E    E G D            +  S  + T++ 
Sbjct: 1227 DTSGKFAIPEDVGSDRP----PDCRMEFDPSEGGKDNFVESSQVVKPAHTESQFQATDLS 1286

Query: 841  IIDDLSPKKLSRQGTLSNGF---DCHHSDYEDSQVG--RVLSS--------GDLHVDRTI 900
              D L    +  Q T  NG        +    +Q+   R+L S        G  + + T 
Sbjct: 1287 --DTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIPDLRLLGSESELNFKGGPTNDEHTT 1346

Query: 901  PISIGDSTLSKLFCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQK 960
             + +   +        +S   + H+    R  +     +SSY         +     G +
Sbjct: 1347 QVQLPSPSFYYSLNKNYSLNSRKHIMAEDRPVY-----VSSY--------RELEWRSGAR 1406

Query: 961  LHIHLADDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGG--ALQPALSS 1020
            L + L  ++ V+  Y+ E +SI+A  L   + +   + S     + + GG  +L  +++ 
Sbjct: 1407 LLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNL 1466

Query: 1021 RSLNKVPSNGSSDSESSISSEEYRFSSF--DRLNLLDSLVSETFKRSDHEGVIKSLAKGK 1080
             SLN + S+ S D   S+SS + + S      L L D     +F      G      K K
Sbjct: 1467 LSLNSL-SDLSVDMSRSLSSADEQVSQLLHSSLYLKDLHARISFTDEGPPG------KVK 1526

Query: 1081 YLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIK 1140
            Y V C Y  +F  LR  CCPSE  FI SL RC  W A+GGKS  FFAK+LDDRFIIK++ 
Sbjct: 1527 YSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVT 1586

Query: 1141 RTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENL 1200
            +TE +SF+KF P YFKY+ ES    + T LAK+LGIYQV+ +  K GKE + D++VMENL
Sbjct: 1587 KTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENL 1646

Query: 1201 SFGRNIIRQYDLKGALHARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIW 1260
             F RN  R YDLKG+  AR+NP T+    VLLDQN V  M +SP++V ++AKR L+RA+W
Sbjct: 1647 LFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSPIFVGSKAKRLLERAVW 1706

Query: 1261 NDTTFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLI---PKNV 1262
            NDT+FL SI+VMDYSLLVGVD E+ ELV GIID++RQYTWDK LETWVK+S +   PKN 
Sbjct: 1707 NDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHLETWVKTSGLLGGPKNS 1726

BLAST of MELO3C013178 vs. TAIR 10
Match: AT4G33240.2 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 661.0 bits (1704), Expect = 2.0e-189
Identity = 473/1349 (35.06%), Postives = 694/1349 (51.45%), Query Frame = 0

Query: 1    MVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPIS-RLSSFNS-MDQENDFTNHVIEMIEG 60
            +V KK+ AH+ M +  + P+L+++ G L    IS +LSSF++ + QE D     +  I+ 
Sbjct: 446  VVCKKNVAHRRMTSKIEKPRLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDS 505

Query: 61   CTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTS-ETLMSQKTR 120
               +++LVEK+ +R  QE +L K ++LVL++K   L+RI+ CTG+ I+ S + L S K  
Sbjct: 506  HNPDILLVEKSVSRFAQEYLLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLG 565

Query: 121  QCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKLVVQ 180
             CD  + +K VE H    +  K   KTLMF +GCP  LGCTILLKGAH DELK+VK V+Q
Sbjct: 566  YCDLFHVEKFVETHVSPCQVAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQ 625

Query: 181  CAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPL----------------- 240
              V  A+ L LETSF+ D+ A    +P     ++A  D  S +                 
Sbjct: 626  YGVFAAYHLALETSFLADEGASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEK 685

Query: 241  EPRSPKVGQAESASDQIEQPTNIHISSGSHEEASNESN--VETVEKLIVSSEPEPYNPAI 300
             P +   G+   A+  +   T    SS +H +   + N  ++  E+L+ + +     P  
Sbjct: 686  SPTTELRGEPHKANGDL---TGNFTSSKTHFQGKLDGNDRIDPSERLLHNLDTVYCKPP- 745

Query: 301  FSGFSSISDSLKRAMGE---SFLLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQ 360
                +S  D L   +     SF +  P +    +    S LSG      +        Y 
Sbjct: 746  -ETITSKDDGLVPTLESRQLSFHVEEPSVQKDQW----SVLSG------ATEQVTDGGYT 805

Query: 361  FDEEVRGSSDGENSVHEQSVSHQSTLEGL--GFHETAPNDTGDTMQKKPPLDSQSILVLM 420
             D  V G+         Q+ + Q  +E     FH +A              D QSILV +
Sbjct: 806  NDSAVIGN---------QNFNRQEQMESSKGDFHPSAS-------------DHQSILVSL 865

Query: 421  SSRNALKGTMCEQSHFSHIVFYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAH 480
            S+R   KG++CE++H   I +Y +FD PLG+FL++NL +Q+  C  C    EAH + Y H
Sbjct: 866  STRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCTMPAEAHIHCYTH 925

Query: 481  YRKQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFG 540
             +  L+I VK+LP  ++LPG+ EGK+WMW RC KC  + G   +T+R+++S AA GLSFG
Sbjct: 926  RQGSLTISVKKLP--ELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVMSDAAWGLSFG 985

Query: 541  KFLELCFSDDTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYSEVAIYTVSMPPQKLEFN 600
            KFLEL FS+    S+ + CGHSL  D L F+G G MVA FRY+ + IY V++PP KL FN
Sbjct: 986  KFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAVTLPPAKLYFN 1045

Query: 601  SSMRQGHLIKEIENVYTKGMLLFSEIAHSLKKIISER--NSSNVNHPQGFLNDFSFVEEM 660
                Q  L KE + V  K  +LF+E+  +L +I ++     S  + P         +  +
Sbjct: 1046 YE-NQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIKLSLEELAGL 1105

Query: 661  LNEERSEFEVKIQNSL--TKKGNLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSLASL-- 720
            L + + E++  +Q  L   K G   +     L +N+L   ++ +S  WD  L   A++  
Sbjct: 1106 LEQRKKEYKDSLQQMLNVVKDGQPTI---DILLINKLRRLIIFDSYAWDECLAGAANMVR 1165

Query: 721  -----------------GVTTNSGTSENVEPEPV-------MLKMNSNINVGPEESESIA 780
                              V+    + E V+  P         L  +++      + +S A
Sbjct: 1166 NNYLEAPKNSAPKVMGRNVSLEKLSDEKVKSIPTHVAICNDSLLQDADYETCLNQGKSFA 1225

Query: 781  ENDDT-KIQQDISVDENVLPLKEIAVEGSDGESGGDEL----------NLPSAIEVTEIQ 840
            +      I +D+  D       +  +E    E G D            +  S  + T++ 
Sbjct: 1226 DTSGKFAIPEDVGSDRP----PDCRMEFDPSEGGKDNFVESSQVVKPAHTESQFQATDLS 1285

Query: 841  IIDDLSPKKLSRQGTLSNGF---DCHHSDYEDSQVG--RVLSS--------GDLHVDRTI 900
              D L    +  Q T  NG        +    +Q+   R+L S        G  + + T 
Sbjct: 1286 --DTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIPDLRLLGSESELNFKGGPTNDEHTT 1345

Query: 901  PISIGDSTLSKLFCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQK 960
             + +   +        +S   + H+    R  +     +SSY         +     G +
Sbjct: 1346 QVQLPSPSFYYSLNKNYSLNSRKHIMAEDRPVY-----VSSY--------RELEWRSGAR 1405

Query: 961  LHIHLADDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGG--ALQPALSS 1020
            L + L  ++ V+  Y+ E +SI+A  L   + +   + S     + + GG  +L  +++ 
Sbjct: 1406 LLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNL 1465

Query: 1021 RSLNKVPSNGSSDSESSISSEEYRFSSF--DRLNLLDSLVSETFKRSDHEGVIKSLAKGK 1080
             SLN + S+ S D   S+SS + + S      L L D     +F      G      K K
Sbjct: 1466 LSLNSL-SDLSVDMSRSLSSADEQVSQLLHSSLYLKDLHARISFTDEGPPG------KVK 1525

Query: 1081 YLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIK 1140
            Y V C Y  +F  LR  CCPSE  FI SL RC  W A+GGKS  FFAK+LDDRFIIK++ 
Sbjct: 1526 YSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVT 1585

Query: 1141 RTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENL 1200
            +TE +SF+KF P YFKY+ ES    + T LAK+LGIYQV+ +  K GKE + D++VMENL
Sbjct: 1586 KTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENL 1645

Query: 1201 SFGRNIIRQYDLKGALHARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIW 1260
             F RN  R YDLKG+  AR+NP T+    VLLDQN V  M +SP++V ++AKR L+RA+W
Sbjct: 1646 LFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSPIFVGSKAKRLLERAVW 1705

Query: 1261 NDTTFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLI---PKNV 1262
            NDT+FL SI+VMDYSLLVGVD E+ ELV GIID++RQYTWDK LETWVK+S +   PKN 
Sbjct: 1706 NDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHLETWVKTSGLLGGPKNS 1725

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK23139.10.0e+0099.77putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucumis melo var. ma... [more]
XP_008447757.10.0e+0097.14PREDICTED: putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucumis m... [more]
XP_011658963.10.0e+0092.66putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucumis sativus] >KA... [more]
XP_038899624.10.0e+0090.82putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Benincasa hispida][more]
XP_022932044.10.0e+0081.13putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D isoform X2 [Cucurbita... [more]
Match NameE-valueIdentityDescription
Q9XID02.1e-29747.30Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thalia... [more]
Q9LUM02.5e-19736.251-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=370... [more]
Q0WUR52.8e-18835.061-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=370... [more]
Q9SSJ82.8e-18836.02Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thalia... [more]
O597222.3e-8124.691-phosphatidylinositol 3-phosphate 5-kinase fab1 OS=Schizosaccharomyces pombe (s... [more]
Match NameE-valueIdentityDescription
A0A5D3DIR80.0e+0099.771-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BI510.0e+0097.141-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo OX=3656 GN=LOC103490... [more]
A0A0A0K7550.0e+0092.661-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_7G... [more]
A0A6J1F0I50.0e+0081.131-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1EVX90.0e+0081.131-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT1G34260.11.5e-29847.30FORMS APLOID AND BINUCLEATE CELLS 1A [more]
AT3G14270.11.8e-19836.25phosphatidylinositol-4-phosphate 5-kinase family protein [more]
AT1G71010.12.0e-18936.02FORMS APLOID AND BINUCLEATE CELLS 1C [more]
AT4G33240.12.0e-18935.061-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... [more]
AT4G33240.22.0e-18935.061-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, coreSMARTSM00330PIPK_2coord: 968..1255
e-value: 2.5E-86
score: 302.8
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, corePFAMPF01504PIP5Kcoord: 1033..1199
e-value: 5.8E-35
score: 120.9
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, corePROSITEPS51455PIPKcoord: 936..1254
score: 57.529236
IPR002423Chaperonin Cpn60/TCP-1 familyPFAMPF00118Cpn60_TCP1coord: 1..191
e-value: 6.1E-14
score: 51.5
IPR027409GroEL-like apical domain superfamilyGENE3D3.50.7.10GroELcoord: 1..163
e-value: 2.0E-30
score: 108.0
IPR027409GroEL-like apical domain superfamilySUPERFAMILY52029GroEL apical domain-likecoord: 1..163
NoneNo IPR availableGENE3D3.30.810.10coord: 1116..1255
e-value: 2.5E-41
score: 143.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 212..241
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 341..382
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 347..365
NoneNo IPR availablePANTHERPTHR457481-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATEDcoord: 1..1283
NoneNo IPR availablePANTHERPTHR45748:SF41-PHOSPHATIDYLINOSITOL-3-PHOSPHATE 5-KINASE FAB1D-RELATEDcoord: 1..1283
NoneNo IPR availableSUPERFAMILY56104SAICAR synthase-likecoord: 978..1254
IPR027484Phosphatidylinositol-4-phosphate 5-kinase, N-terminalGENE3D3.30.800.10Phosphatidylinositol Phosphate Kinase II Betacoord: 936..1114
e-value: 4.5E-33
score: 116.7
IPR0447691-phosphatidylinositol-3-phosphate 5-kinase, PIPK catalytic domainCDDcd17300PIPKc_PIKfyvecoord: 995..1254
e-value: 1.33825E-133
score: 407.667

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C013178.1MELO3C013178.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046488 phosphatidylinositol metabolic process
molecular_function GO:0000285 1-phosphatidylinositol-3-phosphate 5-kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0016307 phosphatidylinositol phosphate kinase activity