MELO3C012935 (gene) Melon (DHL92) v4

Overview
NameMELO3C012935
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionPectinesterase
Locationchr04: 14522400 .. 14524474 (+)
RNA-Seq ExpressionMELO3C012935
SyntenyMELO3C012935
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACATTATTCCAAAGAGATAACTTTGTTAATAACAACGTTGTTGCTGTTGTTTTGTCATGTGAATGTTTCAAAGGCATTGGATTATTGCGAGTTGAATTATAATGATCCTCATAAAGTTGCATACACAATTGTTGTTGATAAATTTGGGAGAGGTAACTTCCACACAATCCAAAGTGCCATTGATTCTGTCCCTTCCCAAAGTAACAGATGGATTAGAATTCAAATTTCTCCTGGCATATATACGTACGGTATATGATCATTTATTTCCCGTTTATTAAAATAACATTTTAGTCCCCATACGTCAAATTCCATCTTATTTTCATCATGTTTAGATTTACTTCTTTTTTTTTTATAAAACTTATAAAGATGTCTATCGATGTCTATCGCTGTCTATCAGTATCTTTTAGTGATAGATGTTAGCTAGAAAAATGAATAGTTTAGTTTGGTTAGCTTGTTTGTGTCTAACACTTGGTGGACACATATCGGATATTTGTTGTTAGTGCAACAAATGTTTTATGCATGTCTAATACATTTTGTGATCAGTAGACTAAAAAAGACACATATACGGCAATATATTAATAACTTTTGAGCATGTGATACATCAAGCTAAGTATTTTAAGCATGTTAATGCATCAACTCATTAGCTATGAAGTTTCTTTTACTATAAGAATGATATTATATTTTTAAGAATGAATATTTTAATAAGCATGTCATCGTCGTGCCATGTCCTAAATTTTTAAATAATGTCAGTGTTGTGGTGTCCCTATTATTTCATATCCATGTCTCATATTTGGATTCGTGTTCTTAGGATTAAGTGATAGTAGTTTATCAGTGTCTGTTTATTATCTATCAATGATATTTTGCTATTATATATTTTTCTATATATATTTGAAAACAAGCATTATACATGTTATAATAACAGGGAAAAAGTAATGATACCCCCAGACAAATCATGCATATTTCTAGATGGAAGTGGGTTTAGAGTGACTGAAATTCAATGGAATGACCATGCAACAACAGCTACTAGTCCCACCTTTACTTCTTATGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGGTATATATATATATATATAACTATTTCATTTCCCTAACTTTCGAATACTGTATATATTTTCTTTGTTTTATGTCTATTATCTGTGATGCAAAATTGGCAGAATACTTATAATGCACTTGGAAGTGTGACTAGACGTGAAGATATAAAACCAGCAATTGCAGCTCTAATCCAAGGAGACAAAGCGATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACTCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTCGGTCAGTCTATCTATAAGGTTATCTTCTAAATAACCTTACATGTACATGCGCTCCAATCGAAGGTTTTGTATATGTGTGTGAGCTTGAAGTCAAATTGAAGTGTTGATTTTTTTCCTTTTTGTTTGTTTGTTTGTGTTGTGTTTAACGCAGGAATGTGTGATAAACATACCAATCAATGCGTATGCTCCATTGCTAAATGAAGGTTTTATAACAGCTCAAGGAAAGGAGAATCCAAGTGAAAGCAGCGGATTCGTATTCATTAGATGCATAGTGAAGGGAAGTGGGAAGGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTGACCCTGCTGGTTGGAATTCTTGGCAACAAGTTGGCCATGAGTAATTAATTTTCTTTTCATCTTTTCATTTCATTTCATTTAATATAATAGTATATTATCTTCTTTTAAATTTTAGTGAGGTCTAAATTATTATTATCATTATCTTTGTTTTGTTTGTAGGAGTAATCTGACGTATTCAGAGACTCGTTGTATTGGACCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGCTTAAGAGATTGGATGTATATCAAATCAGACGATTTACAGATATTTCTTATATTGATCCACAAGGTTATTGGACTTCAAGGCTTCCCGTTTTATCCTAA

mRNA sequence

ATGGAACATTATTCCAAAGAGATAACTTTGTTAATAACAACGTTGTTGCTGTTGTTTTGTCATGTGAATGTTTCAAAGGCATTGGATTATTGCGAGTTGAATTATAATGATCCTCATAAAGTTGCATACACAATTGTTGTTGATAAATTTGGGAGAGGTAACTTCCACACAATCCAAAGTGCCATTGATTCTGTCCCTTCCCAAAGTAACAGATGGATTAGAATTCAAATTTCTCCTGGCATATATACGTACGATGGAAGTGGGTTTAGAGTGACTGAAATTCAATGGAATGACCATGCAACAACAGCTACTAGTCCCACCTTTACTTCTTATGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGAATACTTATAATGCACTTGGAAGTGTGACTAGACGTGAAGATATAAAACCAGCAATTGCAGCTCTAATCCAAGGAGACAAAGCGATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACTCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTCGGTCATCAAATTGAAGTGTTGATTTTTTTCCTTTTTGTTTGTTTGTTTGTGTTGTGTTTAACGCAGGAATGTGTGATAAACATACCAATCAATGCGTATGCTCCATTGCTAAATGAAGGTTTTATAACAGCTCAAGGAAAGGAGAATCCAAGTGAAAGCAGCGGATTCGTATTCATTAGATGCATAGTGAAGGGAAGTGGGAAGGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTGACCCTGCTGGTTGGAATTCTTGGCAACAAGTTGGCCATGAGAGTAATCTGACGTATTCAGAGACTCGTTGTATTGGACCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGCTTAAGAGATTGGATGTATATCAAATCAGACGATTTACAGATATTTCTTATATTGATCCACAAGGTTATTGGACTTCAAGGCTTCCCGTTTTATCCTAA

Coding sequence (CDS)

ATGGAACATTATTCCAAAGAGATAACTTTGTTAATAACAACGTTGTTGCTGTTGTTTTGTCATGTGAATGTTTCAAAGGCATTGGATTATTGCGAGTTGAATTATAATGATCCTCATAAAGTTGCATACACAATTGTTGTTGATAAATTTGGGAGAGGTAACTTCCACACAATCCAAAGTGCCATTGATTCTGTCCCTTCCCAAAGTAACAGATGGATTAGAATTCAAATTTCTCCTGGCATATATACGTACGATGGAAGTGGGTTTAGAGTGACTGAAATTCAATGGAATGACCATGCAACAACAGCTACTAGTCCCACCTTTACTTCTTATGCACAAAACCTTGTAGTACAAGGCATCACTTTTAGGAATACTTATAATGCACTTGGAAGTGTGACTAGACGTGAAGATATAAAACCAGCAATTGCAGCTCTAATCCAAGGAGACAAAGCGATATTTCATAAATGTGGTTTCATTGGTTTGCAAGATACTTTGTGGGATGGTCCTGGCCGTCATCTATTCACTCAGTGCTACATTGAAGGTGTCATTGATGTCATTTCTGGTTTCGGTCATCAAATTGAAGTGTTGATTTTTTTCCTTTTTGTTTGTTTGTTTGTGTTGTGTTTAACGCAGGAATGTGTGATAAACATACCAATCAATGCGTATGCTCCATTGCTAAATGAAGGTTTTATAACAGCTCAAGGAAAGGAGAATCCAAGTGAAAGCAGCGGATTCGTATTCATTAGATGCATAGTGAAGGGAAGTGGGAAGGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTCTGTTTTCTACCTTCTTGCATTGACCCTGCTGGTTGGAATTCTTGGCAACAAGTTGGCCATGAGAGTAATCTGACGTATTCAGAGACTCGTTGTATTGGACCAGGAGCAGATACTTCAAGCCGTGTGCCTTGGCTTAAGAGATTGGATGTATATCAAATCAGACGATTTACAGATATTTCTTATATTGATCCACAAGGTTATTGGACTTCAAGGCTTCCCGTTTTATCCTAA

Protein sequence

MEHYSKEITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQSAIDSVPSQSNRWIRIQISPGIYTYDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLPVLS
Homology
BLAST of MELO3C012935 vs. NCBI nr
Match: XP_016900403.1 (PREDICTED: probable pectinesterase 55 [Cucumis melo])

HSP 1 Score: 734.2 bits (1894), Expect = 5.3e-208
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MEHYSKEITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQS 60
           MEHYSKEITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQS
Sbjct: 1   MEHYSKEITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQS 60

Query: 61  AIDSVPSQSNRWIRIQISPGIYTYDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGI 120
           AIDSVPSQSNRWIRIQISPGIYTYDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGI
Sbjct: 61  AIDSVPSQSNRWIRIQISPGIYTYDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGI 120

Query: 121 TFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIE 180
           TFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIE
Sbjct: 121 TFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIE 180

Query: 181 GVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEGFITAQGKENPS 240
           GVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEGFITAQGKENPS
Sbjct: 181 GVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEGFITAQGKENPS 240

Query: 241 ESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTY 300
           ESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTY
Sbjct: 241 ESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTY 300

Query: 301 SETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLPVLS 351
           SETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLPVLS
Sbjct: 301 SETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLPVLS 350

BLAST of MELO3C012935 vs. NCBI nr
Match: KAE8650757.1 (hypothetical protein Csa_017626 [Cucumis sativus])

HSP 1 Score: 564.7 bits (1454), Expect = 5.6e-157
Identity = 279/354 (78.81%), Postives = 298/354 (84.18%), Query Frame = 0

Query: 1   MEH-YSKEITL-LITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTI 60
           MEH YSKEI+L +ITTL++LFC V VSKALD CELNYNDPHKVAYTIVVDK G+GNF TI
Sbjct: 1   MEHYYSKEISLFIITTLVVLFC-VKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTI 60

Query: 61  QSAIDSVPSQSNRWIRIQISPGIYT-------------YDGSGFRVTEIQWNDHATTATS 120
           QSAI+SVPSQS +WIRIQISPGIYT              DGSG +VTEI WNDH TTA S
Sbjct: 61  QSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAAS 120

Query: 121 PTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTL 180
           PTFT+ AQNLVVQGITFRNTYNA GSV RREDIKPA+AALIQGDK IFHKCGFIGLQDTL
Sbjct: 121 PTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTL 180

Query: 181 WDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPL 240
           WDGPGRHLFTQCYIEGVIDVISGFG  I                 +ECVINIP+NAYAPL
Sbjct: 181 WDGPGRHLFTQCYIEGVIDVISGFGQSI----------------YKECVINIPVNAYAPL 240

Query: 241 LNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAG 300
           LNEGFITAQGKENP+ESSGFVF+RCIV+GSG VFLGRAYRPFSTVIFHLCFLPSCI+PAG
Sbjct: 241 LNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAG 300

Query: 301 WNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQ 340
           WNSW Q GHES+LTYSETRCIGPGADTSSRVPW+ RLD + IR FTDISYIDPQ
Sbjct: 301 WNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQ 337

BLAST of MELO3C012935 vs. NCBI nr
Match: KAA0038126.1 (putative pectinesterase 55 [Cucumis melo var. makuwa] >TYK20481.1 putative pectinesterase 55 [Cucumis melo var. makuwa])

HSP 1 Score: 495.7 bits (1275), Expect = 3.2e-136
Identity = 239/266 (89.85%), Postives = 240/266 (90.23%), Query Frame = 0

Query: 85  DGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAA 144
           DGSGFRVTEIQWNDHATTATSPTFTSYAQNLV       NTYNALGSVTRREDIKPAIAA
Sbjct: 12  DGSGFRVTEIQWNDHATTATSPTFTSYAQNLV-------NTYNALGSVTRREDIKPAIAA 71

Query: 145 LIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCL 204
           LIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG  I           
Sbjct: 72  LIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSI----------- 131

Query: 205 FVLCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAY 264
                 +ECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAY
Sbjct: 132 -----YKECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAY 191

Query: 265 RPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDV 324
           RPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDV
Sbjct: 192 RPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDV 251

Query: 325 YQIRRFTDISYIDPQGYWTSRLPVLS 351
           YQIRRFTDISYIDPQGYWTSRLPVLS
Sbjct: 252 YQIRRFTDISYIDPQGYWTSRLPVLS 254

BLAST of MELO3C012935 vs. NCBI nr
Match: XP_023524602.1 (LOW QUALITY PROTEIN: probable pectinesterase 55 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 473.0 bits (1216), Expect = 2.2e-129
Identity = 232/355 (65.35%), Postives = 278/355 (78.31%), Query Frame = 0

Query: 7   EITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQSAIDSVP 66
           E  LLITTLLLL C +NVSKALD CELN NDP++VAYTIVVDK G GNF TIQSAIDS+P
Sbjct: 2   EQILLITTLLLLLC-LNVSKALD-CELNPNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIP 61

Query: 67  SQSNRWIRIQISPGIYT-------------YDGSGFRVTEIQWNDHATTATSPTFTSYAQ 126
           SQ++RWIRIQIS GIY+              +G+G   TEI+WNDHATTATS TFTS A+
Sbjct: 62  SQNSRWIRIQISSGIYSEKVHISEEKSCIFLEGAGRSSTEIRWNDHATTATSATFTSIAE 121

Query: 127 NLVVQGITFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHL 186
           NLVV+GITF+NTYN  GSV R+EDI PA+AALI+GDK IFHKCGF+GLQDTLWDG GRH 
Sbjct: 122 NLVVKGITFKNTYNVPGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHR 181

Query: 187 FTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEGFITA 246
           +T+CYIEGVIDVISG G  I                 + C INIP++ YAP++  G+ITA
Sbjct: 182 YTECYIEGVIDVISGAGQSI----------------YESCEINIPVDRYAPVIGHGYITA 241

Query: 247 QGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVG 306
           QGKE+ S+++GFVFI C V GSG V+LGRAYRPFSTVI++  FL +CI+PAGW+ W+QVG
Sbjct: 242 QGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACINPAGWDPWEQVG 301

Query: 307 HESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLPV 349
           HE++LTY ETRCIGPGADTS RVPWLK+L + +IRRFT IS++D +G WTS+LP+
Sbjct: 302 HEASLTYLETRCIGPGADTSKRVPWLKKLSIDEIRRFTSISFVDQEG-WTSKLPL 337

BLAST of MELO3C012935 vs. NCBI nr
Match: XP_011652807.1 (probable pectinesterase 55 [Cucumis sativus])

HSP 1 Score: 468.8 bits (1205), Expect = 4.2e-128
Identity = 218/266 (81.95%), Postives = 232/266 (87.22%), Query Frame = 0

Query: 85  DGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAA 144
           DGSG +VTEI WNDH TTA SPTFT+ AQNLVVQGITFRNTYNA GSV RREDIKPA+AA
Sbjct: 47  DGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAA 106

Query: 145 LIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCL 204
           LIQGDK IFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG  I           
Sbjct: 107 LIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSI----------- 166

Query: 205 FVLCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAY 264
                 +ECVINIP+NAYAPLLNEGFITAQGKENP+ESSGFVF+RCIV+GSG VFLGRAY
Sbjct: 167 -----YKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAY 226

Query: 265 RPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDV 324
           RPFSTVIFHLCFLPSCI+PAGWNSW Q GHES+LTYSETRCIGPGADTSSRVPW+ RLD 
Sbjct: 227 RPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDA 286

Query: 325 YQIRRFTDISYIDPQGYWTSRLPVLS 351
           + IR FTDISYIDPQGYWTSR+P+LS
Sbjct: 287 FHIRSFTDISYIDPQGYWTSRIPLLS 296

BLAST of MELO3C012935 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 6.4e-71
Identity = 135/329 (41.03%), Postives = 189/329 (57.45%), Query Frame = 0

Query: 38  PHKV---AYTIVVDKFGRGNFHTIQSAIDSVPSQSNRWIRIQISPGIY------------ 97
           PH +    + ++VD+ G GNF TIQ AIDSVP  +  W  I +  G+Y            
Sbjct: 19  PHLIEAKPFEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPF 78

Query: 98  -TYDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYN--ALGSVTRREDIK 157
               G+G R T ++W+DHA+ A SPTF + A N VV+ ITF N+YN  + G + +   + 
Sbjct: 79  IVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRV- 138

Query: 158 PAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFF 217
           PA+AA I GDK+ F+  GF G+QDTLWD  GRH F +C I+G +D I G G  I      
Sbjct: 139 PAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSI------ 198

Query: 218 LFVCLFVLCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVF 277
                      Q CVI +      P +  G+ITAQG+ N ++++GFVFI C+V G GK +
Sbjct: 199 ----------YQSCVIQVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAY 258

Query: 278 LGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWL 337
           LGRA+RP+S VIF+   L   +DP GW  W   G+E  LTY+E  C G G++TS R  W+
Sbjct: 259 LGRAWRPYSRVIFYNSNLTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWV 318

Query: 338 KRLDVYQIRRFTDISYIDPQGYWTSRLPV 349
           K+L    ++   D+S+I+ +G W   LP+
Sbjct: 319 KKLSASAVQHLADLSFIN-RGGWVEDLPI 328

BLAST of MELO3C012935 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 263.5 bits (672), Expect = 3.5e-69
Identity = 133/322 (41.30%), Postives = 185/322 (57.45%), Query Frame = 0

Query: 40  KVAYTIVVDKFGRGNFHTIQSAIDSVPSQSNRWIRIQISPGIYT-------------YDG 99
           ++AYTI VD  G GNF T+QSAIDS+   ++ WIR+    GIY                G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 100 SGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALI 159
            G   T I+++DH  T  S TFT++A ++V+ GITF+NTYN + +   + +I PA+AA +
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPN--NKREIVPAVAARM 149

Query: 160 QGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFV 219
            GD+ +     F+GLQDTL+DG GRH + +C I G ID I G+G                
Sbjct: 150 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQS-------------- 209

Query: 220 LCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRP 279
             L +EC +N+ +  YAP    G ITA  + +PS+  GFVF  C V G GK  LGRA+  
Sbjct: 210 --LFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGS 269

Query: 280 FSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQ 339
            + VIF    L   + P GW++W+  G+E +LT+ E  C G GADTS RVPWLK+L + +
Sbjct: 270 NARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSE 329

Query: 340 IRRFTDISYIDPQGYWTSRLPV 349
           +  F  +S+ID  G W SR P+
Sbjct: 330 VDGFASVSFIDQDG-WISRFPI 332

BLAST of MELO3C012935 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 5.0e-68
Identity = 133/319 (41.69%), Postives = 186/319 (58.31%), Query Frame = 0

Query: 45  IVVDKFGRGNFHTIQSAIDSVPSQSNRWIRIQISPGIY------TYD-------GSGFRV 104
           + VD+ G GNF TIQ AIDSVP  +  W  I +  G+Y       Y+       G+G R+
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 105 TEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYN--ALGSVTRREDIKPAIAALIQGD 164
           T ++W+DH + A SPTF++ A N VV+ ITF N+YN  + G + +     PA+AALI GD
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPR-TPAVAALIGGD 153

Query: 165 KAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCL 224
           K+ F+  GF G+QDTLWD  GRH F +C I+G +D I G G  I                
Sbjct: 154 KSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSI---------------- 213

Query: 225 TQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFST 284
            Q CVI +      P L  G+ITAQG+ NP +++GF+FI C+V G+G  FLGR +R +S 
Sbjct: 214 YQSCVIQVLGGQLEPGL-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSR 273

Query: 285 VIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRR 344
           VIF+   L   + P GW++W  VGHE+ L ++E  C G GA+   RV W+K+L    I+ 
Sbjct: 274 VIFYNSNLTDVVVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQN 333

Query: 345 FTDISYIDPQGYWTSRLPV 349
             D+S+I+ +G W   LP+
Sbjct: 334 LADLSFIN-RGGWVEDLPI 333

BLAST of MELO3C012935 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.8e-65
Identity = 131/322 (40.68%), Postives = 178/322 (55.28%), Query Frame = 0

Query: 40  KVAYTIVVDKFGRGNFHTIQSAIDSVPSQSNRWIRIQISPGIYT-------------YDG 99
           ++A TI+V+      F T+QSAIDS+P Q+  WIRI IS GIY+               G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 100 SGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALI 159
            G   T I + DH  T TS TFTSY  N+++ GITF+N YN   S       KPA+AA++
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 160 QGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFV 219
            GDK       F G QDTL+D  GRH + +C I G ID I G    I             
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSI------------- 215

Query: 220 LCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRP 279
               + C + + +  Y P    G ITAQG+++P++  GFVF  C V GSGK  LGRA++ 
Sbjct: 216 ---FEGCTLKLRVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKS 275

Query: 280 FSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQ 339
           +S VIF+       I P GW++W+  G E ++T+ E  C G GADTS RVPWL +     
Sbjct: 276 YSRVIFYRSMFSDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKD 335

Query: 340 IRRFTDISYIDPQGYWTSRLPV 349
           + +FT++++ID +G W SRLP+
Sbjct: 336 VLQFTNLTFIDEEG-WLSRLPI 337

BLAST of MELO3C012935 vs. ExPASy Swiss-Prot
Match: O04953 (Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2)

HSP 1 Score: 237.3 bits (604), Expect = 2.7e-61
Identity = 137/342 (40.06%), Postives = 175/342 (51.17%), Query Frame = 0

Query: 10  LLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQSAIDSVPSQS 69
           L I   LLL   +   KALD    N     KV  TIVVD+ G                  
Sbjct: 4   LFIFIALLLSSCIGTLKALDQTCGN-----KVVNTIVVDQAG------------------ 63

Query: 70  NRWIRIQISPGIYTYDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNAL 129
                           G G RVT I +N HA T  S TFTSY  ++VV+ ++  NTYN L
Sbjct: 64  ---------------SGEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRL 123

Query: 130 GSVTRRE----DIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDV 189
            S+T+      DIKPA+A  + GDK+ F+ C F+GLQDT+WD  GRH F  CYIEG ID 
Sbjct: 124 TSLTKANGMSWDIKPAVAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDF 183

Query: 190 ISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGF 249
           I G G  +                 ++C IN    A A  ++ G+ITAQG+ + S+ SGF
Sbjct: 184 IFGSGQSV----------------YEDCHINATAGALASKVSFGYITAQGRSSDSDPSGF 243

Query: 250 VFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRC 309
           VF+R  V GS  V+LGRAY PFS VIF    L S + P GW SW   G+E + TY+E  C
Sbjct: 244 VFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDLSSVVHPEGWYSWHYGGYEMSFTYAEVEC 291

Query: 310 IGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLP 348
            G G+D S RVPW+ +L  +  ++   IS    Q  W S +P
Sbjct: 304 KGAGSDMSRRVPWIDKLHSFYTKQQFSISNFIDQDQWISNIP 291

BLAST of MELO3C012935 vs. ExPASy TrEMBL
Match: A0A1S4DWQ1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 2.6e-208
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MEHYSKEITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQS 60
           MEHYSKEITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQS
Sbjct: 1   MEHYSKEITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQS 60

Query: 61  AIDSVPSQSNRWIRIQISPGIYTYDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGI 120
           AIDSVPSQSNRWIRIQISPGIYTYDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGI
Sbjct: 61  AIDSVPSQSNRWIRIQISPGIYTYDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGI 120

Query: 121 TFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIE 180
           TFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIE
Sbjct: 121 TFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIE 180

Query: 181 GVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEGFITAQGKENPS 240
           GVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEGFITAQGKENPS
Sbjct: 181 GVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEGFITAQGKENPS 240

Query: 241 ESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTY 300
           ESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTY
Sbjct: 241 ESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTY 300

Query: 301 SETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLPVLS 351
           SETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLPVLS
Sbjct: 301 SETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLPVLS 350

BLAST of MELO3C012935 vs. ExPASy TrEMBL
Match: A0A0A0LB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 5.1e-164
Identity = 288/365 (78.90%), Postives = 309/365 (84.66%), Query Frame = 0

Query: 1   MEH-YSKEITL-LITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTI 60
           MEH YSKEI+L +ITTL++LFC V VSKALD CELNYNDPHKVAYTIVVDK G+GNF TI
Sbjct: 1   MEHYYSKEISLFIITTLVVLFC-VKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTI 60

Query: 61  QSAIDSVPSQSNRWIRIQISPGIYT-------------YDGSGFRVTEIQWNDHATTATS 120
           QSAI+SVPSQS +WIRIQISPGIYT              DGSG +VTEI WNDH TTA S
Sbjct: 61  QSAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAAS 120

Query: 121 PTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTL 180
           PTFT+ AQNLVVQGITFRNTYNA GSV RREDIKPA+AALIQGDK IFHKCGFIGLQDTL
Sbjct: 121 PTFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTL 180

Query: 181 WDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPL 240
           WDGPGRHLFTQCYIEGVIDVISGFG  I                 +ECVINIP+NAYAPL
Sbjct: 181 WDGPGRHLFTQCYIEGVIDVISGFGQSI----------------YKECVINIPVNAYAPL 240

Query: 241 LNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAG 300
           LNEGFITAQGKENP+ESSGFVF+RCIV+GSG VFLGRAYRPFSTVIFHLCFLPSCI+PAG
Sbjct: 241 LNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAG 300

Query: 301 WNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSR 351
           WNSW Q GHES+LTYSETRCIGPGADTSSRVPW+ RLD + IR FTDISYIDPQGYWTSR
Sbjct: 301 WNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSR 348

BLAST of MELO3C012935 vs. ExPASy TrEMBL
Match: A0A5D3DA76 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00550 PE=4 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.5e-136
Identity = 239/266 (89.85%), Postives = 240/266 (90.23%), Query Frame = 0

Query: 85  DGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAA 144
           DGSGFRVTEIQWNDHATTATSPTFTSYAQNLV       NTYNALGSVTRREDIKPAIAA
Sbjct: 12  DGSGFRVTEIQWNDHATTATSPTFTSYAQNLV-------NTYNALGSVTRREDIKPAIAA 71

Query: 145 LIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCL 204
           LIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG  I           
Sbjct: 72  LIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSI----------- 131

Query: 205 FVLCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAY 264
                 +ECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAY
Sbjct: 132 -----YKECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAY 191

Query: 265 RPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDV 324
           RPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDV
Sbjct: 192 RPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDV 251

Query: 325 YQIRRFTDISYIDPQGYWTSRLPVLS 351
           YQIRRFTDISYIDPQGYWTSRLPVLS
Sbjct: 252 YQIRRFTDISYIDPQGYWTSRLPVLS 254

BLAST of MELO3C012935 vs. ExPASy TrEMBL
Match: A0A6J1DWQ8 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 2.2e-122
Identity = 225/352 (63.92%), Postives = 260/352 (73.86%), Query Frame = 0

Query: 1   MEHYSKEITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQS 60
           MEHYSK    LI TLL   C  NVS AL  CELNY +P++VAY+IVV+KFGRGNF TIQS
Sbjct: 1   MEHYSKGTIWLIPTLLFA-CLYNVSNAL-ACELNYQNPYRVAYSIVVNKFGRGNFRTIQS 60

Query: 61  AIDSVPSQSNRWIRIQISPGIY-------------TYDGSGFRVTEIQWNDHATTATSPT 120
           AIDS+P  S +WIR+QIS G Y               DG+G RVTEIQWNDH TTATS T
Sbjct: 61  AIDSIPFGSTKWIRVQISAGTYWEKVTIPKGKSCIFIDGAGSRVTEIQWNDHQTTATSAT 120

Query: 121 FTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWD 180
           FTS+A+NLVV+GI FRNTYNA GSV R+EDI PA+AALI GDKA F++C FIGLQDTLWD
Sbjct: 121 FTSFAENLVVRGIAFRNTYNAPGSVRRQEDIAPAVAALIMGDKASFYECRFIGLQDTLWD 180

Query: 181 GPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLN 240
           GPGRH F  CYIEGVIDVISG G  I                 ++C+IN+PI+ YAP+L+
Sbjct: 181 GPGRHHFVNCYIEGVIDVISGSGQSI----------------YEKCMINVPIHQYAPILS 240

Query: 241 EGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWN 300
            G+ITAQGKENP+E SGFVF  C V G+G+V+LGRAYRPFSTVIFH  FL   IDPAGWN
Sbjct: 241 YGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGWN 300

Query: 301 SWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQ 340
            W QVGHE++LTY+E  C GPGA+ S RVPWLKRL   +IR FTDIS+ID +
Sbjct: 301 PWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIRHFTDISFIDQE 334

BLAST of MELO3C012935 vs. ExPASy TrEMBL
Match: A0A067HG98 (Pectinesterase OS=Citrus sinensis OX=2711 GN=CISIN_1g043974mg PE=4 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 1.2e-83
Identity = 169/354 (47.74%), Postives = 214/354 (60.45%), Query Frame = 0

Query: 10  LLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQSAIDSVPSQS 69
           L   TLLLL    NVS ALD C+LN  +P KVAYTI V + G GNF  IQ AI+ +P  +
Sbjct: 8   LFAVTLLLL--SSNVSTALD-CKLNNANPLKVAYTISVSRSGGGNFTKIQDAINFIPVNN 67

Query: 70  NRWIRIQISPGIYT-------------YDGSGFRVTEIQWNDHATTATSPTFTSYAQNLV 129
           ++WIRIQISP IY               +G+  R T+IQW DH TT +SPTFTS ++N+V
Sbjct: 68  DQWIRIQISPEIYQEKVTIPQNKPCIFLEGASSRTTKIQWGDHDTTISSPTFTSLSENVV 127

Query: 130 VQGITFRNTYNALGSVTR---REDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHL 189
            +GI F+NTYN   S  +   R ++K A+AA I GDK+ F+ CGF GLQDTL+D  GRH 
Sbjct: 128 AKGILFQNTYNVPPSYRQNQERMEVKQALAARISGDKSAFYNCGFAGLQDTLFDDHGRHY 187

Query: 190 FTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEGFITA 249
           F QCYIEG ID I G G  I                   C +N+ +  Y P  + G+ITA
Sbjct: 188 FHQCYIEGAIDFIFGNGQSI----------------YHNCAVNVTLKEYLPEKDYGYITA 247

Query: 250 QGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVG 309
           QG+ +  + SGFVF  C   GSGK +LGRAY  FS VI     L   ++P GWN+W  V 
Sbjct: 248 QGRNSTDDPSGFVFTSCQFLGSGKAYLGRAYGAFSRVIIVNSVLTDMVEPQGWNAWNHVE 307

Query: 310 HESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLP 348
           HE N+ Y+E RC G GADTS RV W K L V ++++FTD S+ID  G W ++LP
Sbjct: 308 HEENIEYAEARCRGAGADTSKRVSWEKHLSVDEVKKFTDSSFIDHDG-WIAKLP 341

BLAST of MELO3C012935 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 4.5e-72
Identity = 135/329 (41.03%), Postives = 189/329 (57.45%), Query Frame = 0

Query: 38  PHKV---AYTIVVDKFGRGNFHTIQSAIDSVPSQSNRWIRIQISPGIY------------ 97
           PH +    + ++VD+ G GNF TIQ AIDSVP  +  W  I +  G+Y            
Sbjct: 19  PHLIEAKPFEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPF 78

Query: 98  -TYDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYN--ALGSVTRREDIK 157
               G+G R T ++W+DHA+ A SPTF + A N VV+ ITF N+YN  + G + +   + 
Sbjct: 79  IVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRV- 138

Query: 158 PAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFF 217
           PA+AA I GDK+ F+  GF G+QDTLWD  GRH F +C I+G +D I G G  I      
Sbjct: 139 PAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSI------ 198

Query: 218 LFVCLFVLCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVF 277
                      Q CVI +      P +  G+ITAQG+ N ++++GFVFI C+V G GK +
Sbjct: 199 ----------YQSCVIQVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAY 258

Query: 278 LGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWL 337
           LGRA+RP+S VIF+   L   +DP GW  W   G+E  LTY+E  C G G++TS R  W+
Sbjct: 259 LGRAWRPYSRVIFYNSNLTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWV 318

Query: 338 KRLDVYQIRRFTDISYIDPQGYWTSRLPV 349
           K+L    ++   D+S+I+ +G W   LP+
Sbjct: 319 KKLSASAVQHLADLSFIN-RGGWVEDLPI 328

BLAST of MELO3C012935 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 263.5 bits (672), Expect = 2.5e-70
Identity = 133/322 (41.30%), Postives = 185/322 (57.45%), Query Frame = 0

Query: 40  KVAYTIVVDKFGRGNFHTIQSAIDSVPSQSNRWIRIQISPGIYT-------------YDG 99
           ++AYTI VD  G GNF T+QSAIDS+   ++ WIR+    GIY                G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 100 SGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALI 159
            G   T I+++DH  T  S TFT++A ++V+ GITF+NTYN + +   + +I PA+AA +
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPN--NKREIVPAVAARM 149

Query: 160 QGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFV 219
            GD+ +     F+GLQDTL+DG GRH + +C I G ID I G+G                
Sbjct: 150 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQS-------------- 209

Query: 220 LCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRP 279
             L +EC +N+ +  YAP    G ITA  + +PS+  GFVF  C V G GK  LGRA+  
Sbjct: 210 --LFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGS 269

Query: 280 FSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQ 339
            + VIF    L   + P GW++W+  G+E +LT+ E  C G GADTS RVPWLK+L + +
Sbjct: 270 NARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSE 329

Query: 340 IRRFTDISYIDPQGYWTSRLPV 349
           +  F  +S+ID  G W SR P+
Sbjct: 330 VDGFASVSFIDQDG-WISRFPI 332

BLAST of MELO3C012935 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 259.6 bits (662), Expect = 3.6e-69
Identity = 133/319 (41.69%), Postives = 186/319 (58.31%), Query Frame = 0

Query: 45  IVVDKFGRGNFHTIQSAIDSVPSQSNRWIRIQISPGIY------TYD-------GSGFRV 104
           + VD+ G GNF TIQ AIDSVP  +  W  I +  G+Y       Y+       G+G R+
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 105 TEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYN--ALGSVTRREDIKPAIAALIQGD 164
           T ++W+DH + A SPTF++ A N VV+ ITF N+YN  + G + +     PA+AALI GD
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPR-TPAVAALIGGD 153

Query: 165 KAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCL 224
           K+ F+  GF G+QDTLWD  GRH F +C I+G +D I G G  I                
Sbjct: 154 KSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSI---------------- 213

Query: 225 TQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFST 284
            Q CVI +      P L  G+ITAQG+ NP +++GF+FI C+V G+G  FLGR +R +S 
Sbjct: 214 YQSCVIQVLGGQLEPGL-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSR 273

Query: 285 VIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRR 344
           VIF+   L   + P GW++W  VGHE+ L ++E  C G GA+   RV W+K+L    I+ 
Sbjct: 274 VIFYNSNLTDVVVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQN 333

Query: 345 FTDISYIDPQGYWTSRLPV 349
             D+S+I+ +G W   LP+
Sbjct: 334 LADLSFIN-RGGWVEDLPI 333

BLAST of MELO3C012935 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.3e-66
Identity = 131/322 (40.68%), Postives = 178/322 (55.28%), Query Frame = 0

Query: 40  KVAYTIVVDKFGRGNFHTIQSAIDSVPSQSNRWIRIQISPGIYT-------------YDG 99
           ++A TI+V+      F T+QSAIDS+P Q+  WIRI IS GIY+               G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 100 SGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALI 159
            G   T I + DH  T TS TFTSY  N+++ GITF+N YN   S       KPA+AA++
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 160 QGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFV 219
            GDK       F G QDTL+D  GRH + +C I G ID I G    I             
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSI------------- 215

Query: 220 LCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRP 279
               + C + + +  Y P    G ITAQG+++P++  GFVF  C V GSGK  LGRA++ 
Sbjct: 216 ---FEGCTLKLRVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKS 275

Query: 280 FSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQ 339
           +S VIF+       I P GW++W+  G E ++T+ E  C G GADTS RVPWL +     
Sbjct: 276 YSRVIFYRSMFSDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKD 335

Query: 340 IRRFTDISYIDPQGYWTSRLPV 349
           + +FT++++ID +G W SRLP+
Sbjct: 336 VLQFTNLTFIDEEG-WLSRLPI 337

BLAST of MELO3C012935 vs. TAIR 10
Match: AT5G26810.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 237.3 bits (604), Expect = 1.9e-62
Identity = 137/342 (40.06%), Postives = 175/342 (51.17%), Query Frame = 0

Query: 10  LLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQSAIDSVPSQS 69
           L I   LLL   +   KALD    N     KV  TIVVD+ G                  
Sbjct: 4   LFIFIALLLSSCIGTLKALDQTCGN-----KVVNTIVVDQAG------------------ 63

Query: 70  NRWIRIQISPGIYTYDGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNAL 129
                           G G RVT I +N HA T  S TFTSY  ++VV+ ++  NTYN L
Sbjct: 64  ---------------SGEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRL 123

Query: 130 GSVTRRE----DIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDV 189
            S+T+      DIKPA+A  + GDK+ F+ C F+GLQDT+WD  GRH F  CYIEG ID 
Sbjct: 124 TSLTKANGMSWDIKPAVAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDF 183

Query: 190 ISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGF 249
           I G G  +                 ++C IN    A A  ++ G+ITAQG+ + S+ SGF
Sbjct: 184 IFGSGQSV----------------YEDCHINATAGALASKVSFGYITAQGRSSDSDPSGF 243

Query: 250 VFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRC 309
           VF+R  V GS  V+LGRAY PFS VIF    L S + P GW SW   G+E + TY+E  C
Sbjct: 244 VFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDLSSVVHPEGWYSWHYGGYEMSFTYAEVEC 291

Query: 310 IGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLP 348
            G G+D S RVPW+ +L  +  ++   IS    Q  W S +P
Sbjct: 304 KGAGSDMSRRVPWIDKLHSFYTKQQFSISNFIDQDQWISNIP 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_016900403.15.3e-208100.00PREDICTED: probable pectinesterase 55 [Cucumis melo][more]
KAE8650757.15.6e-15778.81hypothetical protein Csa_017626 [Cucumis sativus][more]
KAA0038126.13.2e-13689.85putative pectinesterase 55 [Cucumis melo var. makuwa] >TYK20481.1 putative pecti... [more]
XP_023524602.12.2e-12965.35LOW QUALITY PROTEIN: probable pectinesterase 55 [Cucurbita pepo subsp. pepo][more]
XP_011652807.14.2e-12881.95probable pectinesterase 55 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q3E9D36.4e-7141.03Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1[more]
Q4PSQ53.5e-6941.30Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2[more]
Q4PSN05.0e-6841.69Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1[more]
O644791.8e-6540.68Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1[more]
O049532.7e-6140.06Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S4DWQ12.6e-208100.00Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1[more]
A0A0A0LB125.1e-16478.90Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1[more]
A0A5D3DA761.5e-13689.85Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00550... [more]
A0A6J1DWQ82.2e-12263.92Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1[more]
A0A067HG981.2e-8347.74Pectinesterase OS=Citrus sinensis OX=2711 GN=CISIN_1g043974mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G18990.14.5e-7241.03Pectin lyase-like superfamily protein [more]
AT2G47280.12.5e-7041.30Pectin lyase-like superfamily protein [more]
AT3G24130.13.6e-6941.69Pectin lyase-like superfamily protein [more]
AT2G19150.11.3e-6640.68Pectin lyase-like superfamily protein [more]
AT5G26810.11.9e-6240.06Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 45..337
e-value: 6.5E-38
score: 130.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 38..348
e-value: 1.3E-70
score: 240.3
NoneNo IPR availablePANTHERPTHR31321:SF82PECTINESTERASEcoord: 9..349
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 9..349
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 41..343

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C012935.1MELO3C012935.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
molecular_function GO:0030599 pectinesterase activity