MELO3C012397.jh1 (gene) Melon (Harukei-3) v1.41

BLAST of MELO3C012397.jh1 vs. NCBI nr
Match: KAA0034694.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK09245.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 878 bits (2268), Expect = 0.0
Identity = 453/466 (97.21%), Postives = 462/466 (99.14%), Query Frame = 0

Query: 18  MCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIGLSGAIITRLFHAFYGAD 77
           MCLYICIGANSQSFANTGSMVTCVKNFPQSRGA+LGILKGYIGLSGAIIT+LFHAFYGAD
Sbjct: 1   MCLYICIGANSQSFANTGSMVTCVKNFPQSRGALLGILKGYIGLSGAIITQLFHAFYGAD 60

Query: 78  AKSLILFIGWLPPAISFASIPIVRIMKVIRQPKELKVFYNFLYISLALAGFLMLMIIVES 137
           AKSLILFIGWLPPA+SFAS+PI+RIMKVIRQP ELKVFYNFLYISLALAGFLMLMIIVES
Sbjct: 61  AKSLILFIGWLPPAVSFASLPIIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVES 120

Query: 138 KTEFTQNQYGGSVAVLLLLLLLPLVVVVIEEYNLWKLKTAPVKSPTPSVQIVTEQLPKTE 197
           KTEFTQNQYGGSVAVLLLLLLLP+VVVVIEEYNLWKLKT+ VKSPTPSVQIVTEQLPKTE
Sbjct: 121 KTEFTQNQYGGSVAVLLLLLLLPIVVVVIEEYNLWKLKTSLVKSPTPSVQIVTEQLPKTE 180

Query: 198 HPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLIVFTSAACGLGGTLTAIDNLGQI 257
           HPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLIVFTSAACGLGGTLTAIDN GQI
Sbjct: 181 HPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLIVFTSAACGLGGTLTAIDNFGQI 240

Query: 258 GMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSEIVLIKYKFPRTLIFSLILLLSCVGHL 317
           GMSLGYPKK ISTFVTLVSIWNYLGRVACGFFSEIVLIKYKFPRTLIFSLILLLSCVGHL
Sbjct: 241 GMSLGYPKKCISTFVTLVSIWNYLGRVACGFFSEIVLIKYKFPRTLIFSLILLLSCVGHL 300

Query: 318 MIAFDVPNGIYVASIVIGFCFGAQWPLIFAIISDLFGLKYYSTLYNFGSVASPIGLYVLN 377
           MIAFDVPNG+YVASIVIGFCFGAQWPLIFAIISDLFGLKYYSTLYNFGSVASPIGLYVLN
Sbjct: 301 MIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISDLFGLKYYSTLYNFGSVASPIGLYVLN 360

Query: 378 VKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGGECFKLSFIVITAVSLMGMFISLILVI 437
           VKVAGNFYDREAEKQLRA+GIIRKAGEELKCYGGECFKLSFIVITAVSLMGMFISLILVI
Sbjct: 361 VKVAGNFYDREAEKQLRARGIIRKAGEELKCYGGECFKLSFIVITAVSLMGMFISLILVI 420

Query: 438 RTRSFYKSDIYKKFRDEAETEVAGNEAAATTGAAEVTEKTIIHRRS 483
           RTRSFYKSDIYKKFRDEAETEVAGNEAAATTGAAEVTEKTIIHRRS
Sbjct: 421 RTRSFYKSDIYKKFRDEAETEVAGNEAAATTGAAEVTEKTIIHRRS 466

BLAST of MELO3C012397.jh1 vs. NCBI nr
Match: XP_011655862.2 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KAE8648847.1 hypothetical protein Csa_008229 [Cucumis sativus])

HSP 1 Score: 831 bits (2147), Expect = 5.10e-300
Identity = 427/483 (88.41%), Postives = 452/483 (93.58%), Query Frame = 0

Query: 1   MIWLAITHRISTPKVWQMCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIG 60
           MIWLAIT+RISTPKVWQMCLYICIGANSQSF+NTGS+VTCVKNFPQSRGAVLGILKGY+G
Sbjct: 105 MIWLAITNRISTPKVWQMCLYICIGANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVG 164

Query: 61  LSGAIITRLFHAFYGADAKSLILFIGWLPPAISFASIPIVRIMKVIRQPKELKVFYNFLY 120
           LSGAIIT+LFHAFYGAD KSLILFI WLP AIS AS+PI+RIMK IRQP ELKVFYNFLY
Sbjct: 165 LSGAIITQLFHAFYGADEKSLILFIAWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLY 224

Query: 121 ISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVVVVIEEYNLWKLKTAPVK 180
           ISLALAGFLML+IIVESKT+FTQNQYGG VAV+LLL   PLV+VV+EEYNLWKLKT  +K
Sbjct: 225 ISLALAGFLMLVIIVESKTKFTQNQYGGIVAVVLLL---PLVIVVMEEYNLWKLKTTLIK 284

Query: 181 SPTPSVQIVTEQLPKTEHPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLIVFTSA 240
           S  PSVQIVTEQLPKTEHPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLI+ TS 
Sbjct: 285 SSNPSVQIVTEQLPKTEHPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLILLTSV 344

Query: 241 ACGLGGTLTAIDNLGQIGMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSEIVLIKYKFP 300
           ACGLGGTLTAIDNLGQIGMSLGYPKKSIS FVTLVSIW+YLGRV CGF SEIVLIKYKFP
Sbjct: 345 ACGLGGTLTAIDNLGQIGMSLGYPKKSISIFVTLVSIWSYLGRVGCGFISEIVLIKYKFP 404

Query: 301 RTLIFSLILLLSCVGHLMIAFDVPNGIYVASIVIGFCFGAQWPLIFAIISDLFGLKYYST 360
           RTLI SLILLLSCVGHLMIAFDVPNG+YVASIVIGFCFGAQWPL+FAIIS+LFGLKYY+T
Sbjct: 405 RTLILSLILLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLVFAIISELFGLKYYAT 464

Query: 361 LYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGGECFKLSFIV 420
           L+ FG+VASP+GLYVLNVKVAGNFYDREA KQL  KGI+RKAG+EL CYGGECFKLSF V
Sbjct: 465 LHVFGAVASPVGLYVLNVKVAGNFYDREARKQLNTKGILRKAGKELNCYGGECFKLSFTV 524

Query: 421 ITAVSLMGMFISLILVIRTRSFYKSDIYKKFRDEAETEVAGNEAAATTGAAEVTEKTIIH 480
           ITAV+LMGMFISLILVIRTRSFYKSDIYKKFRDEAETEVAGNEA AT GA E TEKTII 
Sbjct: 525 ITAVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAETEVAGNEAVATAGAVEETEKTIIQ 584

Query: 481 RRS 483
           RRS
Sbjct: 585 RRS 584

BLAST of MELO3C012397.jh1 vs. NCBI nr
Match: XP_004142500.2 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8648848.1 hypothetical protein Csa_009434 [Cucumis sativus])

HSP 1 Score: 811 bits (2095), Expect = 3.74e-292
Identity = 416/477 (87.21%), Postives = 442/477 (92.66%), Query Frame = 0

Query: 1   MIWLAITHRISTPKVWQMCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIG 60
           MIWLAIT+RISTPKVWQMCLYICIGANSQSFANTGSMVTCV NFP+SRG VLGILKGY G
Sbjct: 105 MIWLAITNRISTPKVWQMCLYICIGANSQSFANTGSMVTCVMNFPESRGVVLGILKGYAG 164

Query: 61  LSGAIITRLFHAFYGADAKSLILFIGWLPPAISFASIPIVRIMKVIRQPKELKVFYNFLY 120
           LSGAIIT+LFHAFYGAD KSL+LFIGWLP A+SFAS+ I+RI+KVIRQP ELKVFYNFLY
Sbjct: 165 LSGAIITQLFHAFYGADTKSLVLFIGWLPTAVSFASLRIIRIIKVIRQPNELKVFYNFLY 224

Query: 121 ISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVVVVIEEYNLWKLKTAPVK 180
           ISLALAGFLMLMIIVESKTEFTQNQYGGS AV+LLLLLLPL++VV+EEY LWKLKTA ++
Sbjct: 225 ISLALAGFLMLMIIVESKTEFTQNQYGGSAAVVLLLLLLPLLIVVMEEYKLWKLKTALIQ 284

Query: 181 SPTPSVQIVTEQLPKTEHPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLIVFTSA 240
           SP PSVQIVTEQLPKTEHPKQE KEPSCW TIFSPP+RGEDFTILQGLFSVDMLI+FTSA
Sbjct: 285 SPNPSVQIVTEQLPKTEHPKQEHKEPSCWTTIFSPPKRGEDFTILQGLFSVDMLILFTSA 344

Query: 241 ACGLGGTLTAIDNLGQIGMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSEIVLIKYKFP 300
           ACG+GGTLTAIDNLGQIG+SLGYPK+SISTFVTLVSIWNYLGRVACGF SEIVL KYK P
Sbjct: 345 ACGMGGTLTAIDNLGQIGVSLGYPKRSISTFVTLVSIWNYLGRVACGFLSEIVLRKYKCP 404

Query: 301 RTLIFSLILLLSCVGHLMIAFDVPNGIYVASIVIGFCFGAQWPLIFAIISDLFGLKYYST 360
           RTLI SLILLLSCVGHLMIAFDVPNG+YVASIVIGFCFGAQWPLIFAIIS+LFGLKYYST
Sbjct: 405 RTLILSLILLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYST 464

Query: 361 LYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGGECFKLSFIV 420
           LYNFGSVASPIGLYVLNVKVAGNFYDREAEKQL AKGIIRKAGEELKC GG CFKLSFIV
Sbjct: 465 LYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLEAKGIIRKAGEELKCIGGACFKLSFIV 524

Query: 421 ITAVSLMGMFISLILVIRTRSFYKSDIYKKFRDEAET-EVAGNEAAATTGAAEVTEK 476
           IT V+L+GM +SLILVIRTRSFY+SDIYKKFR+E ET EVAGN      G  E   K
Sbjct: 525 ITGVTLLGMLVSLILVIRTRSFYRSDIYKKFREEVETTEVAGNRVVEAAGETEERRK 581

BLAST of MELO3C012397.jh1 vs. NCBI nr
Match: XP_008446819.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0034695.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK09246.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 795 bits (2052), Expect = 1.36e-285
Identity = 410/478 (85.77%), Postives = 436/478 (91.21%), Query Frame = 0

Query: 1   MIWLAITHRISTPKVWQMCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIG 60
           MIWLA+T RISTPKVWQMCLYICIGANSQSFANTGS+VTCVKNFP+SRG VLGILKGY+G
Sbjct: 105 MIWLAVTRRISTPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVG 164

Query: 61  LSGAIITRLFHAFYGADAKSLILFIGWLPPAISFASIPIVRIMKVIRQPKELKVFYNFLY 120
           LSGAIIT+LFHAFYG D KSLIL IGWLP AISFAS+  +RIMKVIRQP ELKVFYNFLY
Sbjct: 165 LSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLY 224

Query: 121 ISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVVVVIEEYNLWKLKTAPVK 180
           ISLALAGFLMLMIIVESK EF QN+YGGS AV+LLLL LPL VV+IEEYNLWKLKTA VK
Sbjct: 225 ISLALAGFLMLMIIVESKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVK 284

Query: 181 SPTPSVQIVTEQLPKTEHPKQ-EQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLIVFTS 240
           SP PSVQIVTE+LPKTEHPKQ EQKEPSCW TIF+PP+RGEDFTILQ LFS DMLI+F +
Sbjct: 285 SPNPSVQIVTEKLPKTEHPKQLEQKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIA 344

Query: 241 AACGLGGTLTAIDNLGQIGMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSEIVLIKYKF 300
           A CG+GGTLTAIDNLGQIGMSLGYPK+SISTFVTLVSIWNYLGRVA GF SEIVL KYKF
Sbjct: 345 AICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKF 404

Query: 301 PRTLIFSLILLLSCVGHLMIAFDVPNGIYVASIVIGFCFGAQWPLIFAIISDLFGLKYYS 360
           PR LI SL LLLSCVGHL+IAFDVPNG+YVASIVIGFCFGAQWPLIFAIIS+LFGLKYYS
Sbjct: 405 PRPLILSLTLLLSCVGHLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYS 464

Query: 361 TLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGGECFKLSFI 420
           TLYNFGSVASPIGLY+LNVKVAGNFYDREAEKQL AKGIIRKAGEELKC+GGECFKLSFI
Sbjct: 465 TLYNFGSVASPIGLYILNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFI 524

Query: 421 VITAVSLMGMFISLILVIRTRSFYKSDIYKKFRDEAET-EVAGNEAAATTGAAEVTEK 476
           VITAV+L+GM +SL+LVIRTRSFYKSDIYKKFRDEAET EVAGN      G  E   K
Sbjct: 525 VITAVTLLGMLVSLVLVIRTRSFYKSDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

BLAST of MELO3C012397.jh1 vs. NCBI nr
Match: XP_038893353.1 (LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 793 bits (2048), Expect = 6.85e-285
Identity = 403/480 (83.96%), Postives = 438/480 (91.25%), Query Frame = 0

Query: 1   MIWLAITHRISTPKVWQMCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIG 60
           MIWLA+T RIS PKVWQMC+YICIGANSQSFANTGS+VTCVKNFP+SRG VLGILKGY+G
Sbjct: 105 MIWLAVTRRISAPKVWQMCVYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVG 164

Query: 61  LSGAIITRLFHAFYGADAKSLILFIGWLPPAISFASIPIVRIMKVIRQPKELKVFYNFLY 120
           LSGAIIT+LFHAFYG D KSLIL IGWLP AISFA +  +RI+KVIR P ELKVFYNFLY
Sbjct: 165 LSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIIKVIRHPNELKVFYNFLY 224

Query: 121 ISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVVVVIEEYNLWKLKTAPVK 180
           ISL LAGFLMLMII+ESKTEFTQN+YGGS AV+L+LL LPL VV+IEEYNLWKLKTA +K
Sbjct: 225 ISLGLAGFLMLMIIIESKTEFTQNEYGGSAAVVLVLLFLPLAVVIIEEYNLWKLKTAFIK 284

Query: 181 SPTPSVQIVTEQLPKTEHPKQ----EQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLIV 240
           S  P VQIVTEQLPKTEHPKQ    EQKEPSCW T+FSPPERGEDFTILQ LFS+DMLI+
Sbjct: 285 SSNPCVQIVTEQLPKTEHPKQTPKQEQKEPSCWTTVFSPPERGEDFTILQALFSIDMLIL 344

Query: 241 FTSAACGLGGTLTAIDNLGQIGMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSEIVLIK 300
           F SA CG+GGTLTAIDNLGQIGMSLGYPK+SISTFV+LVSIWNYLGR+A GF SEIV+ K
Sbjct: 345 FLSATCGVGGTLTAIDNLGQIGMSLGYPKRSISTFVSLVSIWNYLGRIASGFVSEIVVTK 404

Query: 301 YKFPRTLIFSLILLLSCVGHLMIAFDVPNGIYVASIVIGFCFGAQWPLIFAIISDLFGLK 360
           YKFPR LI SL LLLSCVGHLMIAFDVPNG+YVAS+VIGFCFGAQWP+IFAIIS+LFGLK
Sbjct: 405 YKFPRPLILSLTLLLSCVGHLMIAFDVPNGLYVASVVIGFCFGAQWPVIFAIISELFGLK 464

Query: 361 YYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGGECFKL 420
           YYSTLYNFGSVASPIGLY+LNVKVAGNFYDREAEKQLRAKGI RKAG++LKC+GGECFKL
Sbjct: 465 YYSTLYNFGSVASPIGLYILNVKVAGNFYDREAEKQLRAKGITRKAGKKLKCFGGECFKL 524

Query: 421 SFIVITAVSLMGMFISLILVIRTRSFYKSDIYKKFRDEAETEVAGNEAAATTGAAEVTEK 476
           SFIVITAV+L+G FISLILVIRT+SFYKSDIYKKFRDEAETEVAGN A A  GA E T+K
Sbjct: 525 SFIVITAVTLLGTFISLILVIRTKSFYKSDIYKKFRDEAETEVAGNGAVAPAGAGEETKK 584

BLAST of MELO3C012397.jh1 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 5.2e-15
Identity = 103/452 (22.79%), Postives = 201/452 (44.47%), Query Frame = 0

Query: 3   WLAITHRISTPKVWQMCLYICIGAN-SQSFANTGSMVTCVKNFPQSRGAVLGILKGYIGL 62
           WL IT+ I+ P  + +    C+ A  S  + NT   + C+++FP +R   L +   + G+
Sbjct: 126 WLVITNIITLP--YSLVFLCCLLAGLSICWFNTACFILCIRHFPNNRALALSLTVSFNGI 185

Query: 63  SGAIITRLFHAFYGADAKSLILFIGWLPPAISFASI-PIVRIMKVIRQP-----KELKVF 122
           S A+ +  F+A   + +   +L    +P  +SFA++ P++    +   P     +     
Sbjct: 186 SAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHV 245

Query: 123 YNFLYISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVVVVIEEYNLWKLK 182
           +  L +   +  F +L  +  S T   +  + G+V +L+  L  PL+V    +Y L  + 
Sbjct: 246 FTILNVLAVITSFHLL--LSSSSTSSARLNFIGAVVLLVFPLCAPLLVYA-RDYFLPVIN 305

Query: 183 TAPVKSPTPSVQIVTEQL--PKTEHPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDM 242
                  +  V +  ++L   KT    +   E        +    G++ +    +  ++ 
Sbjct: 306 ARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKEGNTVRLGDEHSFRLLISRLEF 365

Query: 243 LIVFTSAACGLGGTLTAIDNLGQIGMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSEIV 302
            + + +  CG    L   +NLGQI  SLG   ++ +T VT+ S +++ GR+      + +
Sbjct: 366 WLYYIAYFCGGTIGLVYSNNLGQIAQSLG---QNSTTLVTIYSSFSFFGRLLSA-APDFM 425

Query: 303 LIKYKFPRTLIFSLILLLSCVGHLMIAF--DVPNGIYVASIVIGFCFGAQWPLIFAIISD 362
             +++  RT  F++ LL + +   ++A        +  A+ +IG   G  +    +I SD
Sbjct: 426 HKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSD 485

Query: 363 LFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGG 422
           LFG       +N      PIG  +L   +A + Y+  A     +  I     + + C G 
Sbjct: 486 LFGPNSVGVNHNILITNIPIG-SLLYGYIAASIYEANA-----SPDITPIVSDSIVCIGR 545

Query: 423 ECFKLSFIVITAVSLMGMFISLILVIRTRSFY 444
           +C+  +F+    +S++G+  SL L IRT+  Y
Sbjct: 546 DCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of MELO3C012397.jh1 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 9.3e-04
Identity = 40/160 (25.00%), Postives = 74/160 (46.25%), Query Frame = 0

Query: 267 SISTFVTLVSIWNYLGRVACGFFSEIVLIKYKFPRTLIFSLILLLSCVGHLMI------A 326
           S ST V+L + ++ + R+  GF SE   ++    R ++ S+I L++   HLM+       
Sbjct: 311 SFSTHVSLFATFSTVSRLVVGFSSE--AMESHVSRPVLLSVIALVAACIHLMVPSGIFTV 370

Query: 327 FDVPNGIYVASIVIGFCFGAQWPLIFAIISDLFGLKYYSTLYNFGSVASPIGLYVLNVKV 386
           FD      V +IV GF +G+ + L+  I++ ++G+    T++         G ++L + V
Sbjct: 371 FDNAKYFSVVTIVNGFSYGSSFTLVPTIVTKVWGIANLGTIW---------GSFILALAV 430

Query: 387 AGNFYDREAEKQLRAKGIIRKAGEELKCYGGECFKLSFIV 421
               Y     K   A   +        C G  C+ L+F++
Sbjct: 431 GSLGYGLLFAKVYDAASEVGVGSMSQVCSGVHCYGLTFVI 459

BLAST of MELO3C012397.jh1 vs. ExPASy TrEMBL
Match: A0A5D3CCM6 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002390 PE=4 SV=1)

HSP 1 Score: 878 bits (2268), Expect = 0.0
Identity = 453/466 (97.21%), Postives = 462/466 (99.14%), Query Frame = 0

Query: 18  MCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIGLSGAIITRLFHAFYGAD 77
           MCLYICIGANSQSFANTGSMVTCVKNFPQSRGA+LGILKGYIGLSGAIIT+LFHAFYGAD
Sbjct: 1   MCLYICIGANSQSFANTGSMVTCVKNFPQSRGALLGILKGYIGLSGAIITQLFHAFYGAD 60

Query: 78  AKSLILFIGWLPPAISFASIPIVRIMKVIRQPKELKVFYNFLYISLALAGFLMLMIIVES 137
           AKSLILFIGWLPPA+SFAS+PI+RIMKVIRQP ELKVFYNFLYISLALAGFLMLMIIVES
Sbjct: 61  AKSLILFIGWLPPAVSFASLPIIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVES 120

Query: 138 KTEFTQNQYGGSVAVLLLLLLLPLVVVVIEEYNLWKLKTAPVKSPTPSVQIVTEQLPKTE 197
           KTEFTQNQYGGSVAVLLLLLLLP+VVVVIEEYNLWKLKT+ VKSPTPSVQIVTEQLPKTE
Sbjct: 121 KTEFTQNQYGGSVAVLLLLLLLPIVVVVIEEYNLWKLKTSLVKSPTPSVQIVTEQLPKTE 180

Query: 198 HPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLIVFTSAACGLGGTLTAIDNLGQI 257
           HPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLIVFTSAACGLGGTLTAIDN GQI
Sbjct: 181 HPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLIVFTSAACGLGGTLTAIDNFGQI 240

Query: 258 GMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSEIVLIKYKFPRTLIFSLILLLSCVGHL 317
           GMSLGYPKK ISTFVTLVSIWNYLGRVACGFFSEIVLIKYKFPRTLIFSLILLLSCVGHL
Sbjct: 241 GMSLGYPKKCISTFVTLVSIWNYLGRVACGFFSEIVLIKYKFPRTLIFSLILLLSCVGHL 300

Query: 318 MIAFDVPNGIYVASIVIGFCFGAQWPLIFAIISDLFGLKYYSTLYNFGSVASPIGLYVLN 377
           MIAFDVPNG+YVASIVIGFCFGAQWPLIFAIISDLFGLKYYSTLYNFGSVASPIGLYVLN
Sbjct: 301 MIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISDLFGLKYYSTLYNFGSVASPIGLYVLN 360

Query: 378 VKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGGECFKLSFIVITAVSLMGMFISLILVI 437
           VKVAGNFYDREAEKQLRA+GIIRKAGEELKCYGGECFKLSFIVITAVSLMGMFISLILVI
Sbjct: 361 VKVAGNFYDREAEKQLRARGIIRKAGEELKCYGGECFKLSFIVITAVSLMGMFISLILVI 420

Query: 438 RTRSFYKSDIYKKFRDEAETEVAGNEAAATTGAAEVTEKTIIHRRS 483
           RTRSFYKSDIYKKFRDEAETEVAGNEAAATTGAAEVTEKTIIHRRS
Sbjct: 421 RTRSFYKSDIYKKFRDEAETEVAGNEAAATTGAAEVTEKTIIHRRS 466

BLAST of MELO3C012397.jh1 vs. ExPASy TrEMBL
Match: A0A5D3CFW3 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002400 PE=4 SV=1)

HSP 1 Score: 795 bits (2052), Expect = 6.56e-286
Identity = 410/478 (85.77%), Postives = 436/478 (91.21%), Query Frame = 0

Query: 1   MIWLAITHRISTPKVWQMCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIG 60
           MIWLA+T RISTPKVWQMCLYICIGANSQSFANTGS+VTCVKNFP+SRG VLGILKGY+G
Sbjct: 105 MIWLAVTRRISTPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVG 164

Query: 61  LSGAIITRLFHAFYGADAKSLILFIGWLPPAISFASIPIVRIMKVIRQPKELKVFYNFLY 120
           LSGAIIT+LFHAFYG D KSLIL IGWLP AISFAS+  +RIMKVIRQP ELKVFYNFLY
Sbjct: 165 LSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLY 224

Query: 121 ISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVVVVIEEYNLWKLKTAPVK 180
           ISLALAGFLMLMIIVESK EF QN+YGGS AV+LLLL LPL VV+IEEYNLWKLKTA VK
Sbjct: 225 ISLALAGFLMLMIIVESKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVK 284

Query: 181 SPTPSVQIVTEQLPKTEHPKQ-EQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLIVFTS 240
           SP PSVQIVTE+LPKTEHPKQ EQKEPSCW TIF+PP+RGEDFTILQ LFS DMLI+F +
Sbjct: 285 SPNPSVQIVTEKLPKTEHPKQLEQKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIA 344

Query: 241 AACGLGGTLTAIDNLGQIGMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSEIVLIKYKF 300
           A CG+GGTLTAIDNLGQIGMSLGYPK+SISTFVTLVSIWNYLGRVA GF SEIVL KYKF
Sbjct: 345 AICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKF 404

Query: 301 PRTLIFSLILLLSCVGHLMIAFDVPNGIYVASIVIGFCFGAQWPLIFAIISDLFGLKYYS 360
           PR LI SL LLLSCVGHL+IAFDVPNG+YVASIVIGFCFGAQWPLIFAIIS+LFGLKYYS
Sbjct: 405 PRPLILSLTLLLSCVGHLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYS 464

Query: 361 TLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGGECFKLSFI 420
           TLYNFGSVASPIGLY+LNVKVAGNFYDREAEKQL AKGIIRKAGEELKC+GGECFKLSFI
Sbjct: 465 TLYNFGSVASPIGLYILNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFI 524

Query: 421 VITAVSLMGMFISLILVIRTRSFYKSDIYKKFRDEAET-EVAGNEAAATTGAAEVTEK 476
           VITAV+L+GM +SL+LVIRTRSFYKSDIYKKFRDEAET EVAGN      G  E   K
Sbjct: 525 VITAVTLLGMLVSLVLVIRTRSFYKSDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

BLAST of MELO3C012397.jh1 vs. ExPASy TrEMBL
Match: A0A1S3BG09 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 PE=4 SV=1)

HSP 1 Score: 795 bits (2052), Expect = 6.56e-286
Identity = 410/478 (85.77%), Postives = 436/478 (91.21%), Query Frame = 0

Query: 1   MIWLAITHRISTPKVWQMCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIG 60
           MIWLA+T RISTPKVWQMCLYICIGANSQSFANTGS+VTCVKNFP+SRG VLGILKGY+G
Sbjct: 105 MIWLAVTRRISTPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVG 164

Query: 61  LSGAIITRLFHAFYGADAKSLILFIGWLPPAISFASIPIVRIMKVIRQPKELKVFYNFLY 120
           LSGAIIT+LFHAFYG D KSLIL IGWLP AISFAS+  +RIMKVIRQP ELKVFYNFLY
Sbjct: 165 LSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLY 224

Query: 121 ISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVVVVIEEYNLWKLKTAPVK 180
           ISLALAGFLMLMIIVESK EF QN+YGGS AV+LLLL LPL VV+IEEYNLWKLKTA VK
Sbjct: 225 ISLALAGFLMLMIIVESKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVK 284

Query: 181 SPTPSVQIVTEQLPKTEHPKQ-EQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLIVFTS 240
           SP PSVQIVTE+LPKTEHPKQ EQKEPSCW TIF+PP+RGEDFTILQ LFS DMLI+F +
Sbjct: 285 SPNPSVQIVTEKLPKTEHPKQLEQKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIA 344

Query: 241 AACGLGGTLTAIDNLGQIGMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSEIVLIKYKF 300
           A CG+GGTLTAIDNLGQIGMSLGYPK+SISTFVTLVSIWNYLGRVA GF SEIVL KYKF
Sbjct: 345 AICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKF 404

Query: 301 PRTLIFSLILLLSCVGHLMIAFDVPNGIYVASIVIGFCFGAQWPLIFAIISDLFGLKYYS 360
           PR LI SL LLLSCVGHL+IAFDVPNG+YVASIVIGFCFGAQWPLIFAIIS+LFGLKYYS
Sbjct: 405 PRPLILSLTLLLSCVGHLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYS 464

Query: 361 TLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGGECFKLSFI 420
           TLYNFGSVASPIGLY+LNVKVAGNFYDREAEKQL AKGIIRKAGEELKC+GGECFKLSFI
Sbjct: 465 TLYNFGSVASPIGLYILNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFI 524

Query: 421 VITAVSLMGMFISLILVIRTRSFYKSDIYKKFRDEAET-EVAGNEAAATTGAAEVTEK 476
           VITAV+L+GM +SL+LVIRTRSFYKSDIYKKFRDEAET EVAGN      G  E   K
Sbjct: 525 VITAVTLLGMLVSLVLVIRTRSFYKSDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

BLAST of MELO3C012397.jh1 vs. ExPASy TrEMBL
Match: A0A6J1GZI8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111458291 PE=4 SV=1)

HSP 1 Score: 775 bits (2001), Expect = 4.86e-278
Identity = 398/485 (82.06%), Postives = 432/485 (89.07%), Query Frame = 0

Query: 1   MIWLAITHRISTPKVWQMCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIG 60
           MIWLA+T +I+ PKVWQMCLYICIGANSQSFANTGS+VTCVKNFP+SRG VLGILKGY+G
Sbjct: 105 MIWLAVTRKIAAPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVG 164

Query: 61  LSGAIITRLFHAFYGADAKSLILFIGWLPPAISFASIPIVRIMKVIRQPKELKVFYNFLY 120
           LSGAIIT+LFHAFYG D KSLIL IGWLP AISFA +  +RIMKVIRQP ELKVFYNFLY
Sbjct: 165 LSGAIITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLY 224

Query: 121 ISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVVVVIEEYNLWKLKTAP-- 180
           ISLALAGFLMLMIIVESKTEF+Q+ YGGS AV+LLLL LPL VV+IEEYNLW LKTA   
Sbjct: 225 ISLALAGFLMLMIIVESKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAAT 284

Query: 181 --VKSPTPSVQIVTEQLPKTEHPK-----QEQKEPSCWRTIFSPPERGEDFTILQGLFSV 240
             ++SP   VQIVTE+LPKTEH K     + QKEPSCW TIF+PPERGEDFTILQ LFSV
Sbjct: 285 AAIRSPNHPVQIVTEKLPKTEHQKHIPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSV 344

Query: 241 DMLIVFTSAACGLGGTLTAIDNLGQIGMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSE 300
           DMLI+F +A CG+GGTLTAIDNLGQIGMSLGYPK+SISTFVTLVSIWNYLGRV  GF SE
Sbjct: 345 DMLILFLAAICGVGGTLTAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSE 404

Query: 301 IVLIKYKFPRTLIFSLILLLSCVGHLMIAFDVPNGIYVASIVIGFCFGAQWPLIFAIISD 360
           IVL KYKFPR L+ S+ LL+SCVGHLMIAFDVPNG+YVASIVIGFCFGAQWPLIFAIIS+
Sbjct: 405 IVLTKYKFPRPLMLSMTLLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISE 464

Query: 361 LFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGG 420
           LFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGI+RK GEELKC+GG
Sbjct: 465 LFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGG 524

Query: 421 ECFKLSFIVITAVSLMGMFISLILVIRTRSFYKSDIYKKFRDEAETEVAGNEAAATTGAA 476
           ECFKLSF+VIT V+L+GMF+SLILVIRTRSFYKSDIYKKFRDEAETE+AGN A    G  
Sbjct: 525 ECFKLSFLVITGVTLLGMFVSLILVIRTRSFYKSDIYKKFRDEAETEMAGNGAVVAAGPP 584

BLAST of MELO3C012397.jh1 vs. ExPASy TrEMBL
Match: A0A6J1K186 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489721 PE=4 SV=1)

HSP 1 Score: 768 bits (1983), Expect = 1.99e-275
Identity = 393/481 (81.70%), Postives = 428/481 (88.98%), Query Frame = 0

Query: 1   MIWLAITHRISTPKVWQMCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIG 60
           MIWLA+T +IS PKVWQMCLYICIGANSQSFANTGS+VTCVKNFP+SRG VLGILKGY+G
Sbjct: 105 MIWLAVTGKISAPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVG 164

Query: 61  LSGAIITRLFHAFYGADAKSLILFIGWLPPAISFASIPIVRIMKVIRQPKELKVFYNFLY 120
           LSGAIIT+LFHAFYG D KSLIL IGWLP AISFA +  +RIMKVIRQP ELKVFYNFLY
Sbjct: 165 LSGAIITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLY 224

Query: 121 ISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVVVVIEEYNLWKLKTAP-- 180
           ISLALAGFLMLMII+ESKTEF+Q+ YGGS AV+LLLL LPL VV+IEEYNLW LKTA   
Sbjct: 225 ISLALAGFLMLMIIIESKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAAT 284

Query: 181 ---VKSPTPSVQIVTEQLPKTEHPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLI 240
              ++SP   VQIVTE++PKTEH    QK+PSCW TIF+PPERGEDFTILQ LFSVDM I
Sbjct: 285 AAAIRSPNHPVQIVTEKVPKTEH----QKQPSCWTTIFNPPERGEDFTILQALFSVDMFI 344

Query: 241 VFTSAACGLGGTLTAIDNLGQIGMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSEIVLI 300
           +F +A CG+GGTLTAIDNLGQIGMSLGYPK+SISTFVTLVSIWNYLGRV  GF SEIVL 
Sbjct: 345 LFLAAICGVGGTLTAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLT 404

Query: 301 KYKFPRTLIFSLILLLSCVGHLMIAFDVPNGIYVASIVIGFCFGAQWPLIFAIISDLFGL 360
           KYKFPR L+ S+ LL+SCVGHLMIAFDVPNG+YVASIVIGFCFGAQWPLIFAIIS+LFGL
Sbjct: 405 KYKFPRPLMLSMTLLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGL 464

Query: 361 KYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGGECFK 420
           KYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGI+RK GEELKC+GGECFK
Sbjct: 465 KYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFK 524

Query: 421 LSFIVITAVSLMGMFISLILVIRTRSFYKSDIYKKFRDEAETEVAGNEAAATTGAAEVTE 476
           LSF+VIT V+L+GMF+SLILVIRTRSFYKSDIYKKFRDEAETE+AGN A       E T 
Sbjct: 525 LSFLVITGVTLLGMFVSLILVIRTRSFYKSDIYKKFRDEAETEIAGNGAVVAAEPPEETR 581

BLAST of MELO3C012397.jh1 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 625.2 bits (1611), Expect = 4.5e-179
Identity = 320/488 (65.57%), Postives = 390/488 (79.92%), Query Frame = 0

Query: 1   MIWLAITHRISTPKVWQMCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIG 60
           MIWLA+T RIS P+VW MCLYIC+GANSQSFANTGS+VTCVKNFP+SRG VLGILKGY+G
Sbjct: 102 MIWLAVTERISKPQVWHMCLYICVGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVG 161

Query: 61  LSGAIITRLFHAFYGADAKSLILFIGWLPPAISFASIPIVRIMKVIRQPKELKVFYNFLY 120
           LSGAIIT+L+ AFYG D K LIL IGWLP  +SFA +  +RIMKV RQ  ELKVFYNFLY
Sbjct: 162 LSGAIITQLYRAFYGEDTKELILMIGWLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLY 221

Query: 121 ISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVVVVIEEYNLWKLKTAPVK 180
           ISL LA FLM++II+   + FTQ+++GGS AV+++LLLLP++VV++EE  LWK K   + 
Sbjct: 222 ISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVIVLLLLPIIVVILEEKKLWKEKQVALN 281

Query: 181 SPTPSVQIVTEQLPKTEHPK------QEQKE-------PSCWRTIFSPPERGEDFTILQG 240
            P P + +VTE+ PK +  +      +E KE       PSCW T+F+PPERG+D+TILQ 
Sbjct: 282 DPAP-INVVTEK-PKLDSSEFKDDDGEESKEVVEKVKTPSCWTTVFNPPERGDDYTILQA 341

Query: 241 LFSVDMLIVFTSAACGLGGTLTAIDNLGQIGMSLGYPKKSISTFVTLVSIWNYLGRVACG 300
           LFSVDMLI+F +  CG+GGTLTAIDNLGQIG SLGYPK+S+STFV+LVSIWNY GRV  G
Sbjct: 342 LFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSG 401

Query: 301 FFSEIVLIKYKFPRTLIFSLILLLSCVGHLMIAFDVPNGIYVASIVIGFCFGAQWPLIFA 360
             SEI LIKYKFPR L+ +++LLLSC GHL+IAF+VP G+YVAS++IGFCFGAQWPL+FA
Sbjct: 402 VVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFA 461

Query: 361 IISDLFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIIRKAGEELK 420
           IIS++FGLKYYSTLYNFGSVASPIG Y+LNV+VAG  YD EA KQ +A G  R  G++L 
Sbjct: 462 IISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLN 521

Query: 421 CYGGECFKLSFIVITAVSLMGMFISLILVIRTRSFYKSDIYKKFRDE---AETEVAGNEA 473
           C G  CFKLSFI+I AV+L G+ +S++LVIRT+ FYKSDIYKKFR++   AE E+A   A
Sbjct: 522 CIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKFYKSDIYKKFREKALAAEMEMAAPAA 581

BLAST of MELO3C012397.jh1 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 530.8 bits (1366), Expect = 1.2e-150
Identity = 263/464 (56.68%), Postives = 343/464 (73.92%), Query Frame = 0

Query: 1   MIWLAITHRISTPKVWQMCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIG 60
           MIWL +T +++ PKVWQMCLYICIGANSQ+FANTG++VTCVKNFP+SRG +LG+LKGY+G
Sbjct: 98  MIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALVTCVKNFPESRGVMLGLLKGYVG 157

Query: 61  LSGAIITRLFHAFYGADAKSLILFIGWLPPAISFASIPIVRIMKVIRQPKELKVFYNFLY 120
           LSGAI T+L+ A YG D+KSLIL I WLP A+S   + ++R  KV+RQ  EL VFY FLY
Sbjct: 158 LSGAIFTQLYFAIYGHDSKSLILLIAWLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLY 217

Query: 121 ISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVVVVIEEYNLWKLKTAPVK 180
           IS+ LA FLM M I E +  F++  Y  S  +   LL +PL V V +E  +W +   P++
Sbjct: 218 ISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALLFVPLTVSVKQELEVWNMMKLPIE 277

Query: 181 SPT------PSVQIVTEQLPKTEHPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDML 240
            P+      P  ++  +Q    +   +E++  SC+ T+FSPP RGED+TILQ L S DM+
Sbjct: 278 EPSEVKVEKPKKELDLDQDKAAKVNGEEKETKSCFSTVFSPPPRGEDYTILQALLSTDMI 337

Query: 241 IVFTSAACGLGGTLTAIDNLGQIGMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSEIVL 300
           I+F +  CGLG +LTA+DNLGQIG SLGYP  ++S+FV+LVSIWNY GRV  GF SE +L
Sbjct: 338 ILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLL 397

Query: 301 IKYKFPRTLIFSLILLLSCVGHLMIAFDVPNGIYVASIVIGFCFGAQWPLIFAIISDLFG 360
            KYK PR L+ +L+LLLSC GHL+IAF VP  +Y+ASI++GF FGAQ PL+FAIIS+LFG
Sbjct: 398 AKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFG 457

Query: 361 LKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGGECF 420
           LKYYSTL+N G +ASP+G Y+LNV+V G  YD+EA KQL A+G+ RK  ++L C G +C+
Sbjct: 458 LKYYSTLFNCGQLASPLGSYILNVRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCY 517

Query: 421 KLSFIVITAVSLMGMFISLILVIRTRSFYKSDIYKKFRDEAETE 459
           KL F+++ AV+  G  +SL L IRTR FYK DIYKKFR+  E+E
Sbjct: 518 KLPFLILAAVTFFGALVSLGLAIRTREFYKGDIYKKFRESPESE 561

BLAST of MELO3C012397.jh1 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 2.5e-68
Identity = 161/451 (35.70%), Postives = 257/451 (56.98%), Query Frame = 0

Query: 1   MIWLAITHRISTPKVWQMCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIG 60
           ++WL +T ++    +W + + I +G N +++ NT S+V+C+ NFP+SRG V+GILKG+ G
Sbjct: 98  VVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLVSCIHNFPESRGPVVGILKGFSG 157

Query: 61  LSGAIITRLFHAFYGADAKSLILFIGWLPPAISFASI----PIVRIMKVIRQPKELKVFY 120
           LSGAI+T+++  F  +   S+IL +   PP +  A +    P+ R  +   +  +L+ F 
Sbjct: 158 LSGAILTQVYLMFNPSHDSSVILMVALGPPVVVLALLFIVRPVERSCRTNLRSDDLR-FL 217

Query: 121 NFLYISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVV----VVIEEYNLW 180
                 + LA +L+ +++++S  + TQ     S A+L++ +++P++V    V I   N+ 
Sbjct: 218 AIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVIFMVVPVLVPFSSVFISGNNVT 277

Query: 181 KLKTAPVKSPTPSVQIVTEQLPKTEHPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVD 240
            +K     S     +  T  + +++ P ++++ P            GEDFT+LQ L   D
Sbjct: 278 SVKPEEGTSNVDQHEART-LIERSDRPPEKKRAPCI----------GEDFTLLQALGQAD 337

Query: 241 MLIVFTSAACGLGGTLTAIDNLGQIGMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSEI 300
             ++F S   G+G  +T IDNLGQI  SLGY    I  FV+L+SI N+LGRVA G+FSE+
Sbjct: 338 FWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKI--FVSLISISNFLGRVAGGYFSEL 397

Query: 301 VLIKYKFPRTLIFSLILLLSCVGHLMIAFDVPNGIYVASIVIGFCFGAQWPLIFAIISDL 360
           ++ K   PRTL  S++  +  +G +  A D P  IYV +IVIG  +GA W +  A +SD+
Sbjct: 398 IIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASVSDI 457

Query: 361 FGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGGE 420
           FGLK + +LYNF   A PIG +V +  +A N YD  A KQ           E L C G  
Sbjct: 458 FGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQAGP----TTETESLVCTGSV 517

Query: 421 CFKLSFIVITAVSLMGMFISLILVIRTRSFY 444
           C+ ++  +++ + LM M +SL +V RTR FY
Sbjct: 518 CYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

BLAST of MELO3C012397.jh1 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 2.5e-68
Identity = 161/451 (35.70%), Postives = 257/451 (56.98%), Query Frame = 0

Query: 1   MIWLAITHRISTPKVWQMCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIG 60
           ++WL +T ++    +W + + I +G N +++ NT S+V+C+ NFP+SRG V+GILKG+ G
Sbjct: 98  VVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLVSCIHNFPESRGPVVGILKGFSG 157

Query: 61  LSGAIITRLFHAFYGADAKSLILFIGWLPPAISFASI----PIVRIMKVIRQPKELKVFY 120
           LSGAI+T+++  F  +   S+IL +   PP +  A +    P+ R  +   +  +L+ F 
Sbjct: 158 LSGAILTQVYLMFNPSHDSSVILMVALGPPVVVLALLFIVRPVERSCRTNLRSDDLR-FL 217

Query: 121 NFLYISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVV----VVIEEYNLW 180
                 + LA +L+ +++++S  + TQ     S A+L++ +++P++V    V I   N+ 
Sbjct: 218 AIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVIFMVVPVLVPFSSVFISGNNVT 277

Query: 181 KLKTAPVKSPTPSVQIVTEQLPKTEHPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVD 240
            +K     S     +  T  + +++ P ++++ P            GEDFT+LQ L   D
Sbjct: 278 SVKPEEGTSNVDQHEART-LIERSDRPPEKKRAPCI----------GEDFTLLQALGQAD 337

Query: 241 MLIVFTSAACGLGGTLTAIDNLGQIGMSLGYPKKSISTFVTLVSIWNYLGRVACGFFSEI 300
             ++F S   G+G  +T IDNLGQI  SLGY    I  FV+L+SI N+LGRVA G+FSE+
Sbjct: 338 FWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKI--FVSLISISNFLGRVAGGYFSEL 397

Query: 301 VLIKYKFPRTLIFSLILLLSCVGHLMIAFDVPNGIYVASIVIGFCFGAQWPLIFAIISDL 360
           ++ K   PRTL  S++  +  +G +  A D P  IYV +IVIG  +GA W +  A +SD+
Sbjct: 398 IIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASVSDI 457

Query: 361 FGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIIRKAGEELKCYGGE 420
           FGLK + +LYNF   A PIG +V +  +A N YD  A KQ           E L C G  
Sbjct: 458 FGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQAGP----TTETESLVCTGSV 517

Query: 421 CFKLSFIVITAVSLMGMFISLILVIRTRSFY 444
           C+ ++  +++ + LM M +SL +V RTR FY
Sbjct: 518 CYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

BLAST of MELO3C012397.jh1 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 254.2 bits (648), Expect = 2.1e-67
Identity = 161/486 (33.13%), Postives = 266/486 (54.73%), Query Frame = 0

Query: 2   IWLAITHRISTPKVWQMCLYICIGANSQSFANTGSMVTCVKNFPQSRGAVLGILKGYIGL 61
           +WL +T R     +W MC+ I +G N +++ NTG++V+ V+NFP+SRG V+GILKG+ GL
Sbjct: 98  VWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVSGVQNFPKSRGPVVGILKGFAGL 157

Query: 62  SGAIITRLFHAFYGADAKSLILFIGWLPPAISFASIPIVRIMKVIRQPKELK-VFYNFLY 121
            GAII++++   + ++  SLIL +   P  +    +  +R +   +Q +      + F+Y
Sbjct: 158 GGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCLMFFIRPVGGHKQIRPTDGASFTFIY 217

Query: 122 -ISLALAGFLMLMIIVESKTEFTQNQYGGSVAVLLLLLLLPLVVVV-------------- 181
            + L LA +LM +++++     + N       VL ++L++P++V +              
Sbjct: 218 GVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVILVVPILVPIMTSFFTETNEPDDT 277

Query: 182 IEEYNLWKLKTAPVKSPTPSVQIV---------TEQLPKTEHPK----------QEQKEP 241
           IEE  + K +       TP + +           + LP +E  K          Q   E 
Sbjct: 278 IEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDLLPASERHKRIAHLQAQLMQAAAEG 337

Query: 242 SCWRTIFSPPERGEDFTILQGLFSVDMLIVFTSAACGLGGTLTAIDNLGQIGMSLGYPKK 301
           +        P RGEDFT+ Q L   D  ++F S   G G  LT IDNLGQ+  SLGY   
Sbjct: 338 AVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNT 397

Query: 302 SISTFVTLVSIWNYLGRVACGFFSEIVLIKYKFPRTLIFSLILLLSCVGHLMIAFDVPNG 361
            +   V+++SIWN+LGR+  G+FSE+V+  Y +PR +  ++  L+  VGH+  A+  P  
Sbjct: 398 HV--LVSMISIWNFLGRIGGGYFSELVVRDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGA 457

Query: 362 IYVASIVIGFCFGAQWPLIFAIISDLFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYD 421
           +Y+ +++IG  +GA W ++ A  S+LFGLK +  LYNF ++A+P G  V +  +A + YD
Sbjct: 458 MYIGTLLIGLGYGAHWAIVPATASELFGLKKFGALYNFLTLANPAGSLVFSGMIASSIYD 517

Query: 422 REAEKQLRAKGIIRKAGEELKCYGGECFKLSFIVITAVSLMGMFISLILVIRTRSFYKSD 453
           REAE+Q  A G +    + L+C G  CF L+ ++++   ++   +S+ILV RT+S Y + 
Sbjct: 518 REAERQ--AHGSVFDPDDALRCNGSICFFLTSLIMSGFCIIACMLSMILVRRTKSVY-TH 577