MELO3C012316.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C012316.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
DescriptionPectinesterase
Locationchr10: 1284159 .. 1289794 (-)
RNA-Seq ExpressionMELO3C012316.jh1
SyntenyMELO3C012316.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSstart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAATTTTGATGATTTTTCTAAAAATTTATATCCACATGGACGGTTTATTAAAATTTAATGAAATTAGACCTCAGACGTTTTTGTCTACATCCGCCTTCCGACTAATTTCGCCTAATTTCCGATTTCCGCACTTATTCTCCCGCCCTCTATATAAACCCTCTCTACTTCTTCACTCACTGAGTCACTTCCGAGTTTACAAAACCATGAACTCACTTCCATTCATTTTCCTTCTCCTTCTCTCTCTCAACTTCGCCGGAGCTTCCGTCTCTCCTCCGTCCTCTACCGCCCTCTGTAAATCCACTCTCTACCCTAAGCTCTGCCGCTCCATTCTCTCCTCTATACGCTTCTCCCCTTCCGATCCATACGGCTACGGAAAATTTTCCGTCAAGCAATGTATCAAACAAGCGATCAAAATGTCGACAGTAATCGGCGACTATCTCGACCGAGGACGCGATTCGAGGCTGAATCGGCGAGAGGCCGGCGCTCTGAGTGACTGCAGAGAGCTCTCCGATTTGAATGTGGAATTTCTTCGGTCGATTGAGAGTGTATTGGAAGCGGCAGAGGGAGTCGATGAGGAACTGGTGGAGCGGGTTGAGAGTACTTTGAGCGCGATCGTGACGAACGGCCAGACTTGCGTCGACGGATTGGTGGAATCGAGGAGTAGTTTGGGGAAGGCGCTATCGGCGCCGCTTTTGAGCGCTGGAGAGCTGTACAGTGTGTCGTTGGGATTGGTCTCGAACGCTATGTCGCGGCGGTGGAAGAAGCGTCGGGAGAAGGGAGGCGGAAACGGAGGGGTTCCGGGGGGTGGGCGGAGCAGAGAGGCATTGAACACGCTGATCAAGGTACGATTGATTGAATAATGGGTTGCCGTTGCTGTTTGGTTTCTGTTTGTCTGTTCGTTCGGTTACACGAAACATTGCATTTGCTTTATTCTATGCATTAAATAATTGGGGGAAAGAAAAATTTTGTTTGGACCTAAGCCACTAATTTAGACCTTTTTTTTAATTAGTATCATATATATATATTTCGGACTAATTGCCAAATCCACCTTTTAAATTAGAAGGACGGTTGCAATTTTATCATTAAATTTTTAACTGTTTGAGCATAAAAATTTAGAGTTATGTTTAAACGTAACAAAATAAACTATAATATTTACAAAATATATCAAAAAAAAAATGATTAGATAGATTATAATATTTTGTTATATTTATAACTTTTTTTTTTCTGAAATTTAGTGATTTGAAAGTAATTTTAAAAAAAGTTATACGATCATTAAATTCAGATATTTAAATTTATATAATTTTATATATTTGAGTGTTCAAATTTTATCATTTGGAAATCTAATTTCTTAAAATTTGATGGTACAAGTAAGTATCATAATTGAGAGATTATTGTTATTATTATTTTATATTATCTTATTTTCAGTTAAGAGACAAAATTACAAATTTAGGATTTAATCCGACATAAAATTTTGAATTTGTCTTAAAGTTTAAGTTTGTTTTGTTAAATACTCATTGGGCGTTAAAACTTGATATTCAAACTCTAGTTATATACTTTTGAAAATTTAGGATCAATAGACATGAGCTTTAATATATAACTAAAATTTGTTTTAATTGAAAAAACAATCGAGCATGTATTTAATTGTAAGTAATGGTCGAATTATTATCCTAAAAATAAAAAGTATTTAATAAATTCTTAAATGTTCAGGTAACATTTACAAACGAATTACTCTAACTTTCAATATCATAATTAATATATTCTTTTTAAAGAGAATTGTTATGAATACATATTTTGTACAAAATATATAAAAAACTTAAATAAATTAAAGAAAATTTGATAATTTTGCTATTTTTCTTTTCATATTTTAATTTTAAAAAATATAGTCTTCAACTTTTAGTTTCTCCTGTTAAATTAGATTTGTGAAACAAATTATGAAGTTTAAAGAATTATTAATTCAAGGTTACAAGTTTAGAATTGTATTAAATGTACTTATTAAATATTAATTAAAGTCTATATATATATAGAAAGAAGTTTTTTGAAATGCAAAACCTTTAGAATTTGTGGACTATAAAAATTTTGTATTATGTATCAAAATATTTATAAAATATAATAATTTTTTCTTCTATTAATAATAATATCATATATAGAAATGGGTAAAAGCTTATCGATATTTATCATCGATTGAATTTAAAATTATGTTGTATACAGTAAATATTTTACCAATTTTATTATTATCCCTATCATCCTTCAACTGATATAATTTAGATGTGGAGATAGTTTTTTATTTTGAGAAGGTGTGGAGGAAAATTATGGCAAGTGGGGTGGAGTATAATATAACATAAAAAATTATGAAAAATTTTAAGTTAATCGGCATTTAATTAATGGTGATCACTTGTTAATTGGAATATTAGGATAAATAAACAGGGAAAGTTCCTGTGACTGCAGAGGCATCTTCAACCTTATCCTTAATACTACAAATTCCCAATTTATAAAAGATTAAGTATTTTATTTTTTTATTATTTTTAAGAAAATAATTATTAATTCTTTTTATTAAACTACGTGAAATAAGTTCGTGAGATTCTTGTTGATGGTATCACGAGAGAGATTTTAGTGGGGACGGCAGAGTAGGATTCCCATGTAGAGAATTTCTCAAATGCATGTTACAAATTGAGATGTGGATTCTTTTCCCAAAAATATTCCTACTTTTAATATGTTTATATATTATTTTTATTTTTTTTATTTTTACTTGATATATATTTTGAGTTCGTCGATGAACTTATAATTTTTTTAAATTATTTAATATGTTGTTGGTTAATGAAGGCTTGAACTTAATAGATTAAAAAACTGAGTGGACCGACTAAAACCATCTGGTTGGTTGGAGATAAGGAGAAGTTCGGTTGATGTCGGTTTGGAAAAATCCAATCCAACAACATTCTAGATATATTTTAATTAGGCTGAAATCGACTCAAACTGACCGATTGCTTAATCAATATTTGGTTCGTACTCTTTTACAGTCGATGTCGGTTTGAATATTTACTAAATTGACGATAGTCGATTTAGTGCTGGTATGGTCGAAACCAACCAACCGATGCTCACCCCTAATTAACTTTGTAATATTAGTGGACGGTAGAATTGAGTTTATAGACTATATGCTTTAGTGTTTGACCAAACATTCTTTTAGTACAACAATTGTGAGGATGAAGATCAAACTTTCTGCTTTCAAAATGAAAAGTTATGTCAATTAATTGGTTTAGTTAAGTTGACTTTGCGACGAAACATTATTCTTTTTAACAATTTGAGTTATTGAAATTTGATAAATTTGTAGCTTGTATATGGTTTTTGGTTTTTATTTTTGAAGTTTATAATCACTTCTTCCAACTATAGATTTCTTTTTCTTTTTGCTAGCTAAGAATGGTACCTAATTTCTTCTTTGATGAGCCTGCCACAGTTTTTTTAGTATGAATTAATTACTGATTTTAGAGTTGATTTTAATTTAAATTTTCTGATAAACTTAGTTTATGCTACAATTATCTTAAAATTTAAAGTTGAATCAGGGACTACATAAAATGGAACCATGCAACAACCACTCCACAAAGTGCTCAGGAAGGAAGCGTTTGTTAACTGATTTAGGCAGCTCAGGAATTTTGATAAACAACACTGTGGTTGTTAGCTCAACTGGTGCTGACAACTTCACATCAATTGGAGATGCAATTGCATTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTCATCTATGTCAGGGAAGGATACTATGAAGAATACGTCGTCGTCCCCAAATTCAAAACCAATATAATGCTTATTGGAGACGGCATTAACCGAACCATCATCACCGGGAACCATAACGTCGTTGATGGTTGGACAACTTATAATTCTTCTACCTTCAGTAAGTAGATCACAAATTCAATACTACTGTCTAATCTTGATTTCACTCACTTTTACTTTTTTCTAAAGAGACATAAAAGTCATCTCATCACAAACTTTGCTCTTTGTTTTTCAAAACATTGCAATATTACTCTTGAAGTAGAATTTCGTTGAAATTTTGGATGAAAATTTGGAATTGAAACGATATACAAATGCCTATAACGATATGATAGTGTCAATTTTTGTTCTAAATGGTCACTGTCACATTATTTCAAGTTCCAATTTCATCCAACATTCTAATGGATTCCAACTTTAGTAATAGTTTTGAAACATTTACGAAATGTCAAAGGATAATACGACAATTTTTGAACAAATACGAGTAATTTTAAATAAAAGAACAGCCATGCAATTTGAATGGTGTGGACATATTTTGACATAATCAATTTATTGCAATTCTTATTTTATCTTTCACTACACCCCCCTCCTCCCAGAGGAAAATCCTTTTACCACAATTCTTAAAAAGTTAAATTACAAGGTATCCATTAATTTTTTTTCTCAAGAATTGGAAACTCACCTTTGATATAGTGTTCTTTGAATTTTTAGACAAAAACGAAGACTTAAAAGCAACGATTTCTGTTAGAAAACGATATGTATATGACCTAAGACGGTATGACGGTTTCTAATTTCCGTTCTGTCACTCCTTTTCAATTTCTGTTCAACATTCTAACAAATTTCTACTTCGAAAGGTTTAGAAGCAAAGTTGAAAGGTATTTTTATAGGTCGTTTAAATATTTGTATTTTGCAGCTGTTTGTGGGGATGGATTTGTTGCAATAGATGTGACATTCAGGAACACAGCAGGACCAGAAAAGCACCAAGCAGTAGCATTAAGAAACAGTGCAGATCTCTCAACATTCTACCGCTGTAGTTTTGAAGGATATCAAGACACTCTTTATGTACATTCCCTAAGACAATTCTATAGAGAATGTGATATTTATGGAACTGTTGATTTCATTTTTGGCAATGCAGCTGCAGTTTTCCAGCAATGCAATCTCTATGCAAGAAAGCCACTCCCCAACCAAAAAAACGCCTTCACCGCACAGGGTCGAACCGATCCGAACCAAAACACAGGCATCTCAATCCACAACTGCACCATCAAGGCTGCGCCCGACTGGGTAATGGATTCGAATACAACAACGACAAACTATCTTGGTCGGCCGTGGAAGCAGTACTCGAGGACAGTGTATATGCAATCTTATATTGGGGATTTGATAAGCCCAGTTGGGTGGTTGGAATGGAATGGAACAGTTGGATTGGAGACATTGTACTATGGAGAGTATGAGAATTATGGACCTGGAGCTAATACAAGTTTGAGAGTGAATTGGCCTGGATTTAGCTTGATGAATCTTACACAAGCTATGAATTTTACTGTCTATAATTTCACTATGGGAGATACTTGGTTGCCTTATACTGATATCCCTTTCTCTGGAGGTTTGCTAATTAAGTAAAGTTCTCCTCTCGTTCAAGCTTTTTGATCATTTATCTATGGAGAAATATGTATTCTTGGTTGCTTCAGTGTATTGGTTACTATGAAATCAAGAGAAACCTATAAAGAACAAGCTGGCTGCCGAAGGGAGTTTGGCTTGGTGTCGTTGATACCATCTCTAGATATAGAGATTGAATGCTTGATTTCTTAATTTGTATTTGTTGTATTAAGGAAAAAAAAAAGACTCGTCGATTTGATAAA

mRNA sequence

GAAATTTTGATGATTTTTCTAAAAATTTATATCCACATGGACGGTTTATTAAAATTTAATGAAATTAGACCTCAGACGTTTTTGTCTACATCCGCCTTCCGACTAATTTCGCCTAATTTCCGATTTCCGCACTTATTCTCCCGCCCTCTATATAAACCCTCTCTACTTCTTCACTCACTGAGTCACTTCCGAGTTTACAAAACCATGAACTCACTTCCATTCATTTTCCTTCTCCTTCTCTCTCTCAACTTCGCCGGAGCTTCCGTCTCTCCTCCGTCCTCTACCGCCCTCTGTAAATCCACTCTCTACCCTAAGCTCTGCCGCTCCATTCTCTCCTCTATACGCTTCTCCCCTTCCGATCCATACGGCTACGGAAAATTTTCCGTCAAGCAATGTATCAAACAAGCGATCAAAATGTCGACAGTAATCGGCGACTATCTCGACCGAGGACGCGATTCGAGGCTGAATCGGCGAGAGGCCGGCGCTCTGAGTGACTGCAGAGAGCTCTCCGATTTGAATGTGGAATTTCTTCGGTCGATTGAGAGTGTATTGGAAGCGGCAGAGGGAGTCGATGAGGAACTGGTGGAGCGGGTTGAGAGTACTTTGAGCGCGATCGTGACGAACGGCCAGACTTGCGTCGACGGATTGGTGGAATCGAGGAGTAGTTTGGGGAAGGCGCTATCGGCGCCGCTTTTGAGCGCTGGAGAGCTGTACAGTGTGTCGTTGGGATTGGTCTCGAACGCTATGTCGCGGCGGTGGAAGAAGCGTCGGGAGAAGGGAGGCGGAAACGGAGGGGTTCCGGGGGGTGGGCGGAGCAGAGAGGCATTGAACACGCTGATCAAGGGACTACATAAAATGGAACCATGCAACAACCACTCCACAAAGTGCTCAGGAAGGAAGCGTTTGTTAACTGATTTAGGCAGCTCAGGAATTTTGATAAACAACACTGTGGTTGTTAGCTCAACTGGTGCTGACAACTTCACATCAATTGGAGATGCAATTGCATTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTCATCTATGTCAGGGAAGGATACTATGAAGAATACGTCGTCGTCCCCAAATTCAAAACCAATATAATGCTTATTGGAGACGGCATTAACCGAACCATCATCACCGGGAACCATAACGTCGTTGATGGTTGGACAACTTATAATTCTTCTACCTTCAACAAAAACGAAGACTTAAAAGCAACGATTTCTGTTAGAAAACGATATGTATATGACCTAAGACGGTATGACGCTGTTTGTGGGGATGGATTTGTTGCAATAGATGTGACATTCAGGAACACAGCAGGACCAGAAAAGCACCAAGCAGTAGCATTAAGAAACAGTGCAGATCTCTCAACATTCTACCGCTGTAGTTTTGAAGGATATCAAGACACTCTTTATGTACATTCCCTAAGACAATTCTATAGAGAATGTGATATTTATGGAACTGTTGATTTCATTTTTGGCAATGCAGCTGCAGTTTTCCAGCAATGCAATCTCTATGCAAGAAAGCCACTCCCCAACCAAAAAAACGCCTTCACCGCACAGGGTCGAACCGATCCGAACCAAAACACAGGCATCTCAATCCACAACTGCACCATCAAGGCTGCGCCCGACTGGGTAATGGATTCGAATACAACAACGACAAACTATCTTGGTCGGCCGTGGAAGCAGTACTCGAGGACAGTGTATATGCAATCTTATATTGGGGATTTGATAAGCCCAGTTGGGTGGTTGGAATGGAATGGAACAGTTGGATTGGAGACATTGTACTATGGAGAGTATGAGAATTATGGACCTGGAGCTAATACAAGTTTGAGAGTGAATTGGCCTGGATTTAGCTTGATGAATCTTACACAAGCTATGAATTTTACTGTCTATAATTTCACTATGGGAGATACTTGGTTGCCTTATACTGATATCCCTTTCTCTGGAGGTTTGCTAATTAAGTAAAGTTCTCCTCTCGTTCAAGCTTTTTGATCATTTATCTATGGAGAAATATGTATTCTTGGTTGCTTCAGTGTATTGGTTACTATGAAATCAAGAGAAACCTATAAAGAACAAGCTGGCTGCCGAAGGGAGTTTGGCTTGGTGTCGTTGATACCATCTCTAGATATAGAGATTGAATGCTTGATTTCTTAATTTGTATTTGTTGTATTAAGGAAAAAAAAAAGACTCGTCGATTTGATAAA

Coding sequence (CDS)

ATGATTTTTCTAAAAATTTATATCCACATGGACGGTTTATTAAAATTTAATGAAATTAGACCTCAGACGTTTTTGTCTACATCCGCCTTCCGACTAATTTCGCCTAATTTCCGATTTCCGCACTTATTCTCCCGCCCTCTATATAAACCCTCTCTACTTCTTCACTCACTGAGTCACTTCCGAGTTTACAAAACCATGAACTCACTTCCATTCATTTTCCTTCTCCTTCTCTCTCTCAACTTCGCCGGAGCTTCCGTCTCTCCTCCGTCCTCTACCGCCCTCTGTAAATCCACTCTCTACCCTAAGCTCTGCCGCTCCATTCTCTCCTCTATACGCTTCTCCCCTTCCGATCCATACGGCTACGGAAAATTTTCCGTCAAGCAATGTATCAAACAAGCGATCAAAATGTCGACAGTAATCGGCGACTATCTCGACCGAGGACGCGATTCGAGGCTGAATCGGCGAGAGGCCGGCGCTCTGAGTGACTGCAGAGAGCTCTCCGATTTGAATGTGGAATTTCTTCGGTCGATTGAGAGTGTATTGGAAGCGGCAGAGGGAGTCGATGAGGAACTGGTGGAGCGGGTTGAGAGTACTTTGAGCGCGATCGTGACGAACGGCCAGACTTGCGTCGACGGATTGGTGGAATCGAGGAGTAGTTTGGGGAAGGCGCTATCGGCGCCGCTTTTGAGCGCTGGAGAGCTGTACAGTGTGTCGTTGGGATTGGTCTCGAACGCTATGTCGCGGCGGTGGAAGAAGCGTCGGGAGAAGGGAGGCGGAAACGGAGGGGTTCCGGGGGGTGGGCGGAGCAGAGAGGCATTGAACACGCTGATCAAGGGACTACATAAAATGGAACCATGCAACAACCACTCCACAAAGTGCTCAGGAAGGAAGCGTTTGTTAACTGATTTAGGCAGCTCAGGAATTTTGATAAACAACACTGTGGTTGTTAGCTCAACTGGTGCTGACAACTTCACATCAATTGGAGATGCAATTGCATTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTCATCTATGTCAGGGAAGGATACTATGAAGAATACGTCGTCGTCCCCAAATTCAAAACCAATATAATGCTTATTGGAGACGGCATTAACCGAACCATCATCACCGGGAACCATAACGTCGTTGATGGTTGGACAACTTATAATTCTTCTACCTTCAACAAAAACGAAGACTTAAAAGCAACGATTTCTGTTAGAAAACGATATGTATATGACCTAAGACGGTATGACGCTGTTTGTGGGGATGGATTTGTTGCAATAGATGTGACATTCAGGAACACAGCAGGACCAGAAAAGCACCAAGCAGTAGCATTAAGAAACAGTGCAGATCTCTCAACATTCTACCGCTGTAGTTTTGAAGGATATCAAGACACTCTTTATGTACATTCCCTAAGACAATTCTATAGAGAATGTGATATTTATGGAACTGTTGATTTCATTTTTGGCAATGCAGCTGCAGTTTTCCAGCAATGCAATCTCTATGCAAGAAAGCCACTCCCCAACCAAAAAAACGCCTTCACCGCACAGGGTCGAACCGATCCGAACCAAAACACAGGCATCTCAATCCACAACTGCACCATCAAGGCTGCGCCCGACTGGGTAATGGATTCGAATACAACAACGACAAACTATCTTGGTCGGCCGTGGAAGCAGTACTCGAGGACAGTGTATATGCAATCTTATATTGGGGATTTGATAAGCCCAGTTGGGTGGTTGGAATGGAATGGAACAGTTGGATTGGAGACATTGTACTATGGAGAGTATGAGAATTATGGACCTGGAGCTAATACAAGTTTGAGAGTGAATTGGCCTGGATTTAGCTTGATGAATCTTACACAAGCTATGAATTTTACTGTCTATAATTTCACTATGGGAGATACTTGGTTGCCTTATACTGATATCCCTTTCTCTGGAGGTTTGCTAATTAAGTAA

Protein sequence

MIFLKIYIHMDGLLKFNEIRPQTFLSTSAFRLISPNFRFPHLFSRPLYKPSLLLHSLSHFRVYKTMNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAEGVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNAMSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGSSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKTNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVCGDGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK
Homology
BLAST of MELO3C012316.jh1 vs. NCBI nr
Match: XP_008446714.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Cucumis melo])

HSP 1 Score: 1124 bits (2908), Expect = 0.0
Identity = 564/589 (95.76%), Postives = 564/589 (95.76%), Query Frame = 0

Query: 66  MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 125
           MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 126 VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 185
           VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE
Sbjct: 61  VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 120

Query: 186 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 245
           GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA
Sbjct: 121 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 180

Query: 246 MSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGS 305
           MSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGS
Sbjct: 181 MSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGS 240

Query: 306 SGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKT 365
           SGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKT
Sbjct: 241 SGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKT 300

Query: 366 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVCG 425
           NIMLIGDGINRTIITGNHNVVDGWTTYNSSTF                         VCG
Sbjct: 301 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTF------------------------TVCG 360

Query: 426 DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY 485
           DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY
Sbjct: 361 DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY 420

Query: 486 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV 545
           GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV
Sbjct: 421 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV 480

Query: 546 MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG 605
           MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG
Sbjct: 481 MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG 540

Query: 606 ANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK 654
           ANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK
Sbjct: 541 ANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK 565

BLAST of MELO3C012316.jh1 vs. NCBI nr
Match: XP_004135375.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucumis sativus] >KGN52152.1 hypothetical protein Csa_007825 [Cucumis sativus])

HSP 1 Score: 1090 bits (2819), Expect = 0.0
Identity = 544/589 (92.36%), Postives = 554/589 (94.06%), Query Frame = 0

Query: 66  MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 125
           MNSLPFIFLLLLSLNFAGASVSPP STALCKSTLYPKLCRSILS+IRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPPSTALCKSTLYPKLCRSILSTIRFSPSDPYGYGKFS 60

Query: 126 VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 185
           VKQCIKQA KMSTVIGDYL+RGRDSRLNR EAGALSDCR+LSDLNVEFLRSIE VLEAAE
Sbjct: 61  VKQCIKQATKMSTVIGDYLNRGRDSRLNRPEAGALSDCRDLSDLNVEFLRSIERVLEAAE 120

Query: 186 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 245
           GVDEELVERVES LSAIVTNGQTC+DGLVESRSSLG ALS PLLSAGELYSVSLGLVSNA
Sbjct: 121 GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSGPLLSAGELYSVSLGLVSNA 180

Query: 246 MSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGS 305
           MSRRWKKRREKGGGNGGVPGGGRSRE L+TLIKGLHKMEPCNN STKC GR+RLLTDLGS
Sbjct: 181 MSRRWKKRREKGGGNGGVPGGGRSREPLDTLIKGLHKMEPCNNQSTKCLGRQRLLTDLGS 240

Query: 306 SGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKT 365
           +GILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYV+EGYYEEYVVVPKFKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVKEGYYEEYVVVPKFKT 300

Query: 366 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVCG 425
           NIMLIGDGINRTIITGNHNVVDGWTTYNSSTF                         VCG
Sbjct: 301 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTF------------------------TVCG 360

Query: 426 DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY 485
           DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY
Sbjct: 361 DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY 420

Query: 486 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV 545
           GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV
Sbjct: 421 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV 480

Query: 546 MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG 605
           MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG
Sbjct: 481 MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG 540

Query: 606 ANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK 654
           ANTSLRVNWPGFSL+N+TQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK
Sbjct: 541 ANTSLRVNWPGFSLLNVTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK 565

BLAST of MELO3C012316.jh1 vs. NCBI nr
Match: XP_038891461.1 (probable pectinesterase/pectinesterase inhibitor 25 [Benincasa hispida])

HSP 1 Score: 1023 bits (2646), Expect = 0.0
Identity = 512/590 (86.78%), Postives = 534/590 (90.51%), Query Frame = 0

Query: 66  MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 125
           MNS+PFIFL LL L FAGA  SPP STALCKSTLYPKLCRSILS+IRFSPSDPYGYGKFS
Sbjct: 1   MNSVPFIFLFLLFLGFAGAYDSPPPSTALCKSTLYPKLCRSILSTIRFSPSDPYGYGKFS 60

Query: 126 VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 185
           VKQC+KQAIKMS+VIG YLDRGRDSRLNRREAGALSDC+ELSD NVEFLRSIE VL+AAE
Sbjct: 61  VKQCLKQAIKMSSVIGHYLDRGRDSRLNRREAGALSDCKELSDSNVEFLRSIEGVLDAAE 120

Query: 186 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 245
           GVDEE VERVES LSAIVTNGQTC+DGLVES SS G  LSAPL  AGELYSVSLGLVSN+
Sbjct: 121 GVDEERVERVESILSAIVTNGQTCIDGLVESGSSFGNVLSAPLSIAGELYSVSLGLVSNS 180

Query: 246 MSRRWKKRREKGGGNGG-VPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLG 305
           +SRRWKKRR+KG G+GG  P G R+RE LNTLIKGLHKM  CNN S KCSGRKRLLT+LG
Sbjct: 181 LSRRWKKRRQKGNGDGGNFPAGPRNREPLNTLIKGLHKMGSCNNQSRKCSGRKRLLTNLG 240

Query: 306 SSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFK 365
           S+GILINNTVVVSSTG DNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVV+PKFK
Sbjct: 241 STGILINNTVVVSSTGGDNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFK 300

Query: 366 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVC 425
           TN+MLIGDGINRT+ITGN NV+DGWTTYNSSTF                         VC
Sbjct: 301 TNVMLIGDGINRTVITGNRNVIDGWTTYNSSTF------------------------TVC 360

Query: 426 GDGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDI 485
           GDGFVAID+TFRNTAGPEKHQAVA+RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDI
Sbjct: 361 GDGFVAIDMTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDI 420

Query: 486 YGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDW 545
           YGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTI AAPDW
Sbjct: 421 YGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIMAAPDW 480

Query: 546 VMDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGP 605
           VMDSNTTT NYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGE+ENYGP
Sbjct: 481 VMDSNTTT-NYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEFENYGP 540

Query: 606 GANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK 654
           GANTSLRVNWPG+SLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK
Sbjct: 541 GANTSLRVNWPGYSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK 565

BLAST of MELO3C012316.jh1 vs. NCBI nr
Match: XP_022976564.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima])

HSP 1 Score: 983 bits (2540), Expect = 0.0
Identity = 490/588 (83.33%), Postives = 524/588 (89.12%), Query Frame = 0

Query: 66  MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 125
           MNS PFIFL LLSL  AGAS SPP S  LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 126 VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 185
           V++C+KQAIKMS+VIG+YLDRG DSRLNR+E GAL DCRELSD NVEFL+SI+ VLEAAE
Sbjct: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 186 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 245
           GVDEEL+ER+ES LSAIVTNGQTC+DGLVESRSSLG ALSAPLLSAGELYSVSLGLV N+
Sbjct: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180

Query: 246 MSRRWKKRREKGGGNGG-VPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLG 305
           +SRRWKKR +KG   GG  P   +SRE LNTLIKGLH M  CN  STKCSGRKR+LTDLG
Sbjct: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQ-STKCSGRKRMLTDLG 240

Query: 306 SSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFK 365
           ++GILINNTVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYVR+GYY+EYVVVPK+K
Sbjct: 241 TTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYK 300

Query: 366 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVC 425
           TNIMLIGDGINRT+ITGNHNVVDGWTTYNSSTF                         V 
Sbjct: 301 TNIMLIGDGINRTVITGNHNVVDGWTTYNSSTF------------------------TVY 360

Query: 426 GDGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDI 485
           GDGFVAID+TFRNTAG EKHQAVA+RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+
Sbjct: 361 GDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDV 420

Query: 486 YGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDW 545
           YGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQ+TGISIHNCTI AAP+W
Sbjct: 421 YGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNW 480

Query: 546 VMDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGP 605
           VMD N TTTNYLGRPWK +SRTVYMQSYIGDLISPVGWLEWNGTVGL+TLYYGE+ENYGP
Sbjct: 481 VMDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGP 540

Query: 606 GANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLL 652
           GANTSLRV+W G SLMN+T+AMNFTVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 GANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of MELO3C012316.jh1 vs. NCBI nr
Match: XP_022150485.1 (probable pectinesterase/pectinesterase inhibitor 25 [Momordica charantia])

HSP 1 Score: 981 bits (2537), Expect = 0.0
Identity = 490/587 (83.48%), Postives = 522/587 (88.93%), Query Frame = 0

Query: 66  MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 125
           MNSLP+IFLLLLSL  AGAS SPP S ALCK+TLYPKLC SILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60

Query: 126 VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 185
           VKQC+KQAIKMS+VIG YL RG  SRLN+REAGAL DCREL+DLNVEFLRSIESVLEAAE
Sbjct: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120

Query: 186 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 245
           GVDE++VERVES LSAIVTNGQTC+DGLVES+SSLG ALSAPL +AGELYSVSLGLV N+
Sbjct: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180

Query: 246 MSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGS 305
           +SRRWKKRR+        PGGG +RE L TLIKGL KM  CN  ST CSGR+RLLTDLG+
Sbjct: 181 LSRRWKKRRQS------FPGGGGNREPLITLIKGLRKMGSCNQ-STICSGRERLLTDLGT 240

Query: 306 SGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKT 365
           +GI INNTVVVSSTGADNFTSIGDAIAF+PNNSMP+DGYFVIYVREGYYEEYVVVPK+KT
Sbjct: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKT 300

Query: 366 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVCG 425
           NIMLIGDGINRT+ITGNH+VVDGWTTYNSSTF                         VCG
Sbjct: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTF------------------------TVCG 360

Query: 426 DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY 485
           DGFVAIDVTFRNTAGPEKHQAVA+RNSADLSTFYRCSFEGYQDTLYVHS RQFYRECDIY
Sbjct: 361 DGFVAIDVTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIY 420

Query: 486 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV 545
           GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQ+TGISIHNCTI+AAPDW 
Sbjct: 421 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWA 480

Query: 546 MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG 605
           MDSN T  NYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGT GLETLYYGE+EN+GPG
Sbjct: 481 MDSNATIANYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPG 540

Query: 606 ANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLL 652
           ANTS+RVNWPG+SLMN TQAMNFTVYNFTMGDTWLPYTDIPF+GGL+
Sbjct: 541 ANTSMRVNWPGYSLMNATQAMNFTVYNFTMGDTWLPYTDIPFNGGLV 556

BLAST of MELO3C012316.jh1 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 8.5e-194
Identity = 345/572 (60.31%), Postives = 422/572 (73.78%), Query Frame = 0

Query: 90  SSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKQCIKQAIKMSTVIGDYLDR--G 149
           S +  CKST YPKLCR+IL++++ SPSDPY YGKF++KQC+KQA ++S VI  Y  R   
Sbjct: 78  SPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVES 137

Query: 150 RDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAEGVDEELVERVESTLSAIVTNGQ 209
           +       E GA++DC ELS+L+V +L ++ + L+ A+ +   LVE V S LS +VTN Q
Sbjct: 138 KPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGVVTNQQ 197

Query: 210 TCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNAMSRRWKKRREKGGGNGGVPGGG 269
           TC+DGLVE++S    A+ +P+ +   LYS+SLGLVS+A++R  K+ +      G + GGG
Sbjct: 198 TCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFK---ASKGKILGGG 257

Query: 270 RS--REALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGSS---GILINNTVVVSSTGAD 329
            S  RE L TLIKGL K   C+N    C    R L +LG +    IL++  V+V    +D
Sbjct: 258 NSTYREPLETLIKGLRK--TCDN-DKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSD 317

Query: 330 NFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKTNIMLIGDGINRTIITGN 389
           NFT+I DAIA APNN+ P+DGYFVIY REG YEEY+VVP  K N+ML+GDGIN+TIITGN
Sbjct: 318 NFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGN 377

Query: 390 HNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVCGDGFVAIDVTFRNTAGPE 449
           HNVVDGWTTYN S+F                        AV G+ F+A+DVTFRNTAGPE
Sbjct: 378 HNVVDGWTTYNCSSF------------------------AVVGERFMAVDVTFRNTAGPE 437

Query: 450 KHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQC 509
           KHQAVALRN+A+ S+FYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAA+FQ C
Sbjct: 438 KHQAVALRNNAEGSSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNC 497

Query: 510 NLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQ 569
           N+YARKP+  QKNA TA GR DPNQNTGISI NCTIKAAPD   +  +  T +LGRPWK 
Sbjct: 498 NIYARKPMAKQKNAITAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMT-FLGRPWKP 557

Query: 570 YSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNL 629
           YSRTV+MQSYI D++ PVGWLEWNGT+GL+T+YYGEY N+GPGANT+ RV W G++L+NL
Sbjct: 558 YSRTVFMQSYISDIVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNL 617

Query: 630 TQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK 655
            +AMNFTVYNFTMGDTWLP TDIPF GGLL K
Sbjct: 618 AEAMNFTVYNFTMGDTWLPQTDIPFYGGLLSK 618

BLAST of MELO3C012316.jh1 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 2.0e-187
Identity = 341/573 (59.51%), Postives = 410/573 (71.55%), Query Frame = 0

Query: 87  SPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKQCIKQAIKMSTVIGDYLDR 146
           SP      CKST YPKLCR+ILS+++ SPSDPY YGKF++KQC+KQA ++S VI  +  R
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 147 --GRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAEGVDEELVERVESTLSAIVT 206
                      E  A++DC EL++L+VE+L ++   L+AAE +   LV+RV S L  +VT
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVT 199

Query: 207 NGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNAMSRRWKKRREKGGGNGGVP 266
           N QTC+DGLV+++S    A+  PL +   LYSVSLGLVS+A++R  K+ +   G  G + 
Sbjct: 200 NQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYK---GSKGKIF 259

Query: 267 GGGRS--REALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGSS---GILINNTVVVSST 326
           GGG    RE L TLIK L K   C+     C    R L +LG +    IL+   V V   
Sbjct: 260 GGGNKPVREPLETLIKVLRK--TCDK-GKDCRKANRNLGELGETSGGSILVREAVTVGPY 319

Query: 327 GADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKTNIMLIGDGINRTII 386
             DNF +I +A+A APN++ P+ GYFVIY R G YEEYVV+   K NIMLIGDGIN+TII
Sbjct: 320 ETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTII 379

Query: 387 TGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVCGDGFVAIDVTFRNTA 446
           +GNH+ +DGWTTYNSSTF                        AV GD FVA+DVTFRNTA
Sbjct: 380 SGNHSFIDGWTTYNSSTF------------------------AVVGDRFVAVDVTFRNTA 439

Query: 447 GPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVF 506
           GPEKHQAVA+RN+AD STFYRCSFEGYQDTLYVHSLRQFYRECDIYGT+DFIFGNAAA+F
Sbjct: 440 GPEKHQAVAVRNNADGSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIF 499

Query: 507 QQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRP 566
           Q CN+YARKP+ NQKNA TA GRTDPNQ TGISI NCTI AAPD   D  +T T +LGRP
Sbjct: 500 QNCNIYARKPMANQKNAVTAHGRTDPNQKTGISIINCTIGAAPDLAADPKSTMT-FLGRP 559

Query: 567 WKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSL 626
           WK YSRTVY+QSYI D++ PVGWLEWNGT GL+T+ YGEY+N+GPGA+TS RV W G+SL
Sbjct: 560 WKPYSRTVYIQSYISDVVQPVGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSL 619

Query: 627 MNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLL 653
           +NL QAMNFTVYNFT+GDTWLP TDIPF GGLL
Sbjct: 620 LNLVQAMNFTVYNFTLGDTWLPQTDIPFYGGLL 621

BLAST of MELO3C012316.jh1 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 501.1 bits (1289), Expect = 1.9e-140
Identity = 283/595 (47.56%), Postives = 376/595 (63.19%), Query Frame = 0

Query: 72  IFLLLLSLNFAGASVSPP-SSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKQCI 131
           +FL L +L  A  ++ P  SS+ +CK+T  PK C+S+    + S  D   YG+FS+++ +
Sbjct: 11  LFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVREYGRFSLRKSL 70

Query: 132 KQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFL-RSIESV--LEAAEGV 191
            Q+ K +  I  YL R  ++ L++   GAL DCR L+ L  ++L  S E+V    +++ +
Sbjct: 71  TQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 130

Query: 192 DEELVERVESTLSAIVTNGQTCVDGLVESRSS---LGKALSAPLLSAGELYSVSLGLVSN 251
                + +++ LSA +TN QTC+DG+  + SS   +   ++ PL++  +L+SVSL L   
Sbjct: 131 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLAL--- 190

Query: 252 AMSRRW--KKRREKGGGNGGVPGGGRS-----REALNTLIKGLHKMEPCNNHSTKCSGRK 311
             ++ W  KK+++    +   P    S     R   N  +  L   E         S RK
Sbjct: 191 -FTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALP-LKMTEHTRAVYESLSRRK 250

Query: 312 RLLTDLGSSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEY 371
               D   + +L+++ V V+  G  NFT+I +A+  APN +    GYFVIYV  G YEE 
Sbjct: 251 LADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEEN 310

Query: 372 VVVPKFKTNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDL 431
           VV+ K K  +M+IGDGINRT++TGN NVVDGWTT+NS+TF                    
Sbjct: 311 VVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATF-------------------- 370

Query: 432 RRYDAVCGDGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQ 491
               AV    FVA+++TFRNTAGPEKHQAVA+R+SADLS FY CSFE YQDTLY HSLRQ
Sbjct: 371 ----AVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQ 430

Query: 492 FYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCT 551
           FYRECDIYGTVDFIFGNAA VFQ CNLY R+P+ NQ NA TAQGRTDPNQNTGISIHNCT
Sbjct: 431 FYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCT 490

Query: 552 IKAAPDWVMDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYG 611
           IK A D ++ SN T   YLGRPWK+YSRTV+MQSYI +++ PVGW EWNG   L TLYY 
Sbjct: 491 IKPADD-LVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYA 550

Query: 612 EYENYGPGANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLL 653
           EY N G G++T+ RV WPG+ ++N T A NFTV NF +GD W+  + +P+  GLL
Sbjct: 551 EYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of MELO3C012316.jh1 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 4.6e-131
Identity = 271/609 (44.50%), Postives = 367/609 (60.26%), Query Frame = 0

Query: 70  PFIFLLLLSL---NFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSV 129
           P   L+ LS    +   +S +  +S+ +CK+T  PK C+S+      S  +   YG FS+
Sbjct: 4   PIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFSI 63

Query: 130 KQCIKQAIKMSTVIGDYLDRGRDSRLNRREA-GALSDCRELSDLNVEF-LRSIESVLEAA 189
           ++ + Q+ K    +  Y+   R++ L++     AL DCR L+ L +++ L S E+V + +
Sbjct: 64  RKSLSQSRKFIRTVDRYIK--RNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTS 123

Query: 190 -----EGVDEELVERVESTLSAIVTNGQTCVDGLVESRS-----SLGKALSAPLLSAGEL 249
                + +     + +++ LSA +TN QTC++GL  + S     ++   ++ PL++  +L
Sbjct: 124 AKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKL 183

Query: 250 YSVSLGLVSNAMSRRW-KKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKC 309
             VSL L     ++ W  K++++ G     P  G S       +     +       TK 
Sbjct: 184 LGVSLAL----FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKA 243

Query: 310 ---SGRKRLLTDLGSSG-------ILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDG 369
              S  +R L D  S+G       +LI++ V VS  G  NFT+I  A+A APNN+    G
Sbjct: 244 VYESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAG 303

Query: 370 YFVIYVREGYYEEYVVVPKFKTNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDL 429
           +F+IYV  G YEEY+ + K K  +M+IGDGIN+T++TGN +VVDGWTT+NS+TF      
Sbjct: 304 FFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATF------ 363

Query: 430 KATISVRKRYVYDLRRYDAVCGDGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSF 489
                             AV    FVA+++TFRNTAGPEKHQAVALR+ AD S FY CSF
Sbjct: 364 ------------------AVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSF 423

Query: 490 EGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRT 549
           E YQDTLY HSLRQFYRECD+YGTVDFIFGNAA VFQ CNLY RKP+PNQ NA TAQGR+
Sbjct: 424 EAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRS 483

Query: 550 DPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWL 609
           DPNQNTG SI NCTIK A D ++ SN T   YLGRPWK+YSRTVYMQSYI   + PVGW 
Sbjct: 484 DPNQNTGTSIQNCTIKPADD-LVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWR 543

Query: 610 EWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYT 653
           EWNG   L TLYY EY N GPG+NT+ RV WPG+ ++N T A NFTV    +   W+  T
Sbjct: 544 EWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKT 578

BLAST of MELO3C012316.jh1 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 465.7 bits (1197), Expect = 8.7e-130
Identity = 266/604 (44.04%), Postives = 353/604 (58.44%), Query Frame = 0

Query: 66  MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 125
           ++SLP  F +   +   G + SP +   +C+    P  CRS+L +    P D Y YG+ S
Sbjct: 14  LSSLPSPF-ISAQIPAIGNATSPSN---ICRFAPDPSYCRSVLPN---QPGDIYSYGRLS 73

Query: 126 VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 185
           +++ + +A +  ++I   LDR +     +   GAL DC+ L+ L +++L S     ++ +
Sbjct: 74  LRRSLSRARRFISMIDAELDR-KGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTK 133

Query: 186 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 245
            +     E V + LSA +TN QTC++GL  + S  G  LS  L +  +LY VSL L    
Sbjct: 134 TLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENG--LSGDLFNDTKLYGVSLAL---- 193

Query: 246 MSRRWKKRREK---------------GGGNGGVP--GGGRSREALNTLIKGLHKMEPCNN 305
            S+ W  RR++               G  NG +P     R+R   NT+ +          
Sbjct: 194 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTR---------- 253

Query: 306 HSTKCSGRKRLLTDLGSSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIY 365
                  RK L +D  +  + +++ V V   G  NFT+I  AIA APN +   +GYF+IY
Sbjct: 254 -------RKLLQSD--ADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIY 313

Query: 366 VREGYYEEYVVVPKFKTNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATIS 425
           V  G YEEYV VPK K  +M+IGDGIN+T+ITGN +VVDGWTT+NS+TF           
Sbjct: 314 VTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATF----------- 373

Query: 426 VRKRYVYDLRRYDAVCGDGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQD 485
                         + G  F+ +++T RNTAGP K QAVALR+  DLS FY CSFE YQD
Sbjct: 374 -------------ILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQD 433

Query: 486 TLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQN 545
           TLY HSLRQFYRECD+YGTVDFIFGNAA V Q CNLY R+P   Q N  TAQGRTDPNQN
Sbjct: 434 TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQN 493

Query: 546 TGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGT 605
           TG +IH CTI+ A D +  SN T   YLGRPWK+YSRTV MQ+YI   + P GW  W+G 
Sbjct: 494 TGTAIHGCTIRPADD-LATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGD 553

Query: 606 VGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFS 653
             L TLYY EY N GPG++T+ RV WPG+ ++N T A NFTV NF +G+ W+  T +PF 
Sbjct: 554 FALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFV 559

BLAST of MELO3C012316.jh1 vs. ExPASy TrEMBL
Match: A0A1S3BFR9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103489353 PE=3 SV=1)

HSP 1 Score: 1124 bits (2908), Expect = 0.0
Identity = 564/589 (95.76%), Postives = 564/589 (95.76%), Query Frame = 0

Query: 66  MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 125
           MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 126 VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 185
           VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE
Sbjct: 61  VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 120

Query: 186 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 245
           GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA
Sbjct: 121 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 180

Query: 246 MSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGS 305
           MSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGS
Sbjct: 181 MSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGS 240

Query: 306 SGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKT 365
           SGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKT
Sbjct: 241 SGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKT 300

Query: 366 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVCG 425
           NIMLIGDGINRTIITGNHNVVDGWTTYNSSTF                         VCG
Sbjct: 301 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTF------------------------TVCG 360

Query: 426 DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY 485
           DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY
Sbjct: 361 DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY 420

Query: 486 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV 545
           GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV
Sbjct: 421 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV 480

Query: 546 MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG 605
           MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG
Sbjct: 481 MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG 540

Query: 606 ANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK 654
           ANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK
Sbjct: 541 ANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK 565

BLAST of MELO3C012316.jh1 vs. ExPASy TrEMBL
Match: A0A0A0KUN8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G612810 PE=3 SV=1)

HSP 1 Score: 1090 bits (2819), Expect = 0.0
Identity = 544/589 (92.36%), Postives = 554/589 (94.06%), Query Frame = 0

Query: 66  MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 125
           MNSLPFIFLLLLSLNFAGASVSPP STALCKSTLYPKLCRSILS+IRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPPSTALCKSTLYPKLCRSILSTIRFSPSDPYGYGKFS 60

Query: 126 VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 185
           VKQCIKQA KMSTVIGDYL+RGRDSRLNR EAGALSDCR+LSDLNVEFLRSIE VLEAAE
Sbjct: 61  VKQCIKQATKMSTVIGDYLNRGRDSRLNRPEAGALSDCRDLSDLNVEFLRSIERVLEAAE 120

Query: 186 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 245
           GVDEELVERVES LSAIVTNGQTC+DGLVESRSSLG ALS PLLSAGELYSVSLGLVSNA
Sbjct: 121 GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSGPLLSAGELYSVSLGLVSNA 180

Query: 246 MSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGS 305
           MSRRWKKRREKGGGNGGVPGGGRSRE L+TLIKGLHKMEPCNN STKC GR+RLLTDLGS
Sbjct: 181 MSRRWKKRREKGGGNGGVPGGGRSREPLDTLIKGLHKMEPCNNQSTKCLGRQRLLTDLGS 240

Query: 306 SGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKT 365
           +GILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYV+EGYYEEYVVVPKFKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVKEGYYEEYVVVPKFKT 300

Query: 366 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVCG 425
           NIMLIGDGINRTIITGNHNVVDGWTTYNSSTF                         VCG
Sbjct: 301 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTF------------------------TVCG 360

Query: 426 DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY 485
           DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY
Sbjct: 361 DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY 420

Query: 486 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV 545
           GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV
Sbjct: 421 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV 480

Query: 546 MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG 605
           MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG
Sbjct: 481 MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG 540

Query: 606 ANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK 654
           ANTSLRVNWPGFSL+N+TQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK
Sbjct: 541 ANTSLRVNWPGFSLLNVTQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK 565

BLAST of MELO3C012316.jh1 vs. ExPASy TrEMBL
Match: A0A6J1INW0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1)

HSP 1 Score: 983 bits (2540), Expect = 0.0
Identity = 490/588 (83.33%), Postives = 524/588 (89.12%), Query Frame = 0

Query: 66  MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 125
           MNS PFIFL LLSL  AGAS SPP S  LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 126 VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 185
           V++C+KQAIKMS+VIG+YLDRG DSRLNR+E GAL DCRELSD NVEFL+SI+ VLEAAE
Sbjct: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 186 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 245
           GVDEEL+ER+ES LSAIVTNGQTC+DGLVESRSSLG ALSAPLLSAGELYSVSLGLV N+
Sbjct: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180

Query: 246 MSRRWKKRREKGGGNGG-VPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLG 305
           +SRRWKKR +KG   GG  P   +SRE LNTLIKGLH M  CN  STKCSGRKR+LTDLG
Sbjct: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQ-STKCSGRKRMLTDLG 240

Query: 306 SSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFK 365
           ++GILINNTVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYVR+GYY+EYVVVPK+K
Sbjct: 241 TTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYK 300

Query: 366 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVC 425
           TNIMLIGDGINRT+ITGNHNVVDGWTTYNSSTF                         V 
Sbjct: 301 TNIMLIGDGINRTVITGNHNVVDGWTTYNSSTF------------------------TVY 360

Query: 426 GDGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDI 485
           GDGFVAID+TFRNTAG EKHQAVA+RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+
Sbjct: 361 GDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDV 420

Query: 486 YGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDW 545
           YGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQ+TGISIHNCTI AAP+W
Sbjct: 421 YGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNW 480

Query: 546 VMDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGP 605
           VMD N TTTNYLGRPWK +SRTVYMQSYIGDLISPVGWLEWNGTVGL+TLYYGE+ENYGP
Sbjct: 481 VMDLNETTTNYLGRPWKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGP 540

Query: 606 GANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLL 652
           GANTSLRV+W G SLMN+T+AMNFTVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 GANTSLRVDWDGHSLMNITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of MELO3C012316.jh1 vs. ExPASy TrEMBL
Match: A0A6J1D8L4 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1)

HSP 1 Score: 981 bits (2537), Expect = 0.0
Identity = 490/587 (83.48%), Postives = 522/587 (88.93%), Query Frame = 0

Query: 66  MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 125
           MNSLP+IFLLLLSL  AGAS SPP S ALCK+TLYPKLC SILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60

Query: 126 VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 185
           VKQC+KQAIKMS+VIG YL RG  SRLN+REAGAL DCREL+DLNVEFLRSIESVLEAAE
Sbjct: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120

Query: 186 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 245
           GVDE++VERVES LSAIVTNGQTC+DGLVES+SSLG ALSAPL +AGELYSVSLGLV N+
Sbjct: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180

Query: 246 MSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGS 305
           +SRRWKKRR+        PGGG +RE L TLIKGL KM  CN  ST CSGR+RLLTDLG+
Sbjct: 181 LSRRWKKRRQS------FPGGGGNREPLITLIKGLRKMGSCNQ-STICSGRERLLTDLGT 240

Query: 306 SGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKT 365
           +GI INNTVVVSSTGADNFTSIGDAIAF+PNNSMP+DGYFVIYVREGYYEEYVVVPK+KT
Sbjct: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKT 300

Query: 366 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVCG 425
           NIMLIGDGINRT+ITGNH+VVDGWTTYNSSTF                         VCG
Sbjct: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTF------------------------TVCG 360

Query: 426 DGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIY 485
           DGFVAIDVTFRNTAGPEKHQAVA+RNSADLSTFYRCSFEGYQDTLYVHS RQFYRECDIY
Sbjct: 361 DGFVAIDVTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIY 420

Query: 486 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWV 545
           GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQ+TGISIHNCTI+AAPDW 
Sbjct: 421 GTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWA 480

Query: 546 MDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPG 605
           MDSN T  NYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGT GLETLYYGE+EN+GPG
Sbjct: 481 MDSNATIANYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPG 540

Query: 606 ANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLL 652
           ANTS+RVNWPG+SLMN TQAMNFTVYNFTMGDTWLPYTDIPF+GGL+
Sbjct: 541 ANTSMRVNWPGYSLMNATQAMNFTVYNFTMGDTWLPYTDIPFNGGLV 556

BLAST of MELO3C012316.jh1 vs. ExPASy TrEMBL
Match: A0A6J1GYC3 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458594 PE=3 SV=1)

HSP 1 Score: 968 bits (2503), Expect = 0.0
Identity = 489/591 (82.74%), Postives = 523/591 (88.49%), Query Frame = 0

Query: 66  MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 125
           MNS+PFIFLL LSL  AGAS S P S  LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSVPFIFLLFLSLGLAGASDSAPPSAFLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 126 VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 185
           VK+C+KQAIKMS+VIG+YL+RG DSRLNR+E GAL DCRELSD NVEFL+SI+ VLEAAE
Sbjct: 61  VKKCLKQAIKMSSVIGEYLNRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 186 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 245
           GVDEELVER+ES LSAIVTNGQTC+DGLVESRSSLG ALSAPLLSAGELYSVSLGLV N+
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180

Query: 246 MSRRWKKRREKGGGNGG-VPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLG 305
           +SRRWKKRR+KG G GG  P   +SRE LNTLIKGLH M  CN  STKC GRKRLLTDLG
Sbjct: 181 LSRRWKKRRQKGDGEGGNFPAAAQSREPLNTLIKGLH-MSSCNQ-STKCWGRKRLLTDLG 240

Query: 306 SSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFK 365
           ++GILINNTVVVSSTGADNFTSIGDAI+FAPNNSM QDGYFVIYVR+GYY+EYV+VPK+K
Sbjct: 241 TTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMLQDGYFVIYVRQGYYDEYVIVPKYK 300

Query: 366 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVC 425
           TNIMLIGDGINRT+ITGNHNVVDGWTTYNSSTF                         V 
Sbjct: 301 TNIMLIGDGINRTVITGNHNVVDGWTTYNSSTF------------------------TVY 360

Query: 426 GDGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDI 485
           GDGFVAID+TFRNTAG EKHQAVA+RNSADLSTFYRCSFEGYQDTLYVHS+RQFYRECD+
Sbjct: 361 GDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDV 420

Query: 486 YGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDW 545
           YGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQ+TGISIHNCTIKAAP+W
Sbjct: 421 YGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPEW 480

Query: 546 VMDSNTTTT---NYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYEN 605
           VMD N TTT   NYLGRPWK +SRTVYMQSYIGDLISPVGWLEWNGTVGL+TLYYGE+EN
Sbjct: 481 VMDLNATTTTTMNYLGRPWKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFEN 540

Query: 606 YGPGANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLL 652
           YG GANTS RVNW G SLMN+T+AMNFTVYNFTMGDTWLPYTDIPFSGGLL
Sbjct: 541 YGAGANTSSRVNWGGHSLMNITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 565

BLAST of MELO3C012316.jh1 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 678.3 bits (1749), Expect = 6.0e-195
Identity = 345/572 (60.31%), Postives = 422/572 (73.78%), Query Frame = 0

Query: 90  SSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKQCIKQAIKMSTVIGDYLDR--G 149
           S +  CKST YPKLCR+IL++++ SPSDPY YGKF++KQC+KQA ++S VI  Y  R   
Sbjct: 78  SPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVES 137

Query: 150 RDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAEGVDEELVERVESTLSAIVTNGQ 209
           +       E GA++DC ELS+L+V +L ++ + L+ A+ +   LVE V S LS +VTN Q
Sbjct: 138 KPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGVVTNQQ 197

Query: 210 TCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNAMSRRWKKRREKGGGNGGVPGGG 269
           TC+DGLVE++S    A+ +P+ +   LYS+SLGLVS+A++R  K+ +      G + GGG
Sbjct: 198 TCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFK---ASKGKILGGG 257

Query: 270 RS--REALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGSS---GILINNTVVVSSTGAD 329
            S  RE L TLIKGL K   C+N    C    R L +LG +    IL++  V+V    +D
Sbjct: 258 NSTYREPLETLIKGLRK--TCDN-DKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSD 317

Query: 330 NFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKTNIMLIGDGINRTIITGN 389
           NFT+I DAIA APNN+ P+DGYFVIY REG YEEY+VVP  K N+ML+GDGIN+TIITGN
Sbjct: 318 NFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGN 377

Query: 390 HNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVCGDGFVAIDVTFRNTAGPE 449
           HNVVDGWTTYN S+F                        AV G+ F+A+DVTFRNTAGPE
Sbjct: 378 HNVVDGWTTYNCSSF------------------------AVVGERFMAVDVTFRNTAGPE 437

Query: 450 KHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQC 509
           KHQAVALRN+A+ S+FYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAA+FQ C
Sbjct: 438 KHQAVALRNNAEGSSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNC 497

Query: 510 NLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQ 569
           N+YARKP+  QKNA TA GR DPNQNTGISI NCTIKAAPD   +  +  T +LGRPWK 
Sbjct: 498 NIYARKPMAKQKNAITAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMT-FLGRPWKP 557

Query: 570 YSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNL 629
           YSRTV+MQSYI D++ PVGWLEWNGT+GL+T+YYGEY N+GPGANT+ RV W G++L+NL
Sbjct: 558 YSRTVFMQSYISDIVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNL 617

Query: 630 TQAMNFTVYNFTMGDTWLPYTDIPFSGGLLIK 655
            +AMNFTVYNFTMGDTWLP TDIPF GGLL K
Sbjct: 618 AEAMNFTVYNFTMGDTWLPQTDIPFYGGLLSK 618

BLAST of MELO3C012316.jh1 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 657.1 bits (1694), Expect = 1.4e-188
Identity = 341/573 (59.51%), Postives = 410/573 (71.55%), Query Frame = 0

Query: 87  SPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKQCIKQAIKMSTVIGDYLDR 146
           SP      CKST YPKLCR+ILS+++ SPSDPY YGKF++KQC+KQA ++S VI  +  R
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 147 --GRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAEGVDEELVERVESTLSAIVT 206
                      E  A++DC EL++L+VE+L ++   L+AAE +   LV+RV S L  +VT
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVT 199

Query: 207 NGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNAMSRRWKKRREKGGGNGGVP 266
           N QTC+DGLV+++S    A+  PL +   LYSVSLGLVS+A++R  K+ +   G  G + 
Sbjct: 200 NQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYK---GSKGKIF 259

Query: 267 GGGRS--REALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGSS---GILINNTVVVSST 326
           GGG    RE L TLIK L K   C+     C    R L +LG +    IL+   V V   
Sbjct: 260 GGGNKPVREPLETLIKVLRK--TCDK-GKDCRKANRNLGELGETSGGSILVREAVTVGPY 319

Query: 327 GADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKTNIMLIGDGINRTII 386
             DNF +I +A+A APN++ P+ GYFVIY R G YEEYVV+   K NIMLIGDGIN+TII
Sbjct: 320 ETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTII 379

Query: 387 TGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDLRRYDAVCGDGFVAIDVTFRNTA 446
           +GNH+ +DGWTTYNSSTF                        AV GD FVA+DVTFRNTA
Sbjct: 380 SGNHSFIDGWTTYNSSTF------------------------AVVGDRFVAVDVTFRNTA 439

Query: 447 GPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVF 506
           GPEKHQAVA+RN+AD STFYRCSFEGYQDTLYVHSLRQFYRECDIYGT+DFIFGNAAA+F
Sbjct: 440 GPEKHQAVAVRNNADGSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIF 499

Query: 507 QQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRP 566
           Q CN+YARKP+ NQKNA TA GRTDPNQ TGISI NCTI AAPD   D  +T T +LGRP
Sbjct: 500 QNCNIYARKPMANQKNAVTAHGRTDPNQKTGISIINCTIGAAPDLAADPKSTMT-FLGRP 559

Query: 567 WKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSL 626
           WK YSRTVY+QSYI D++ PVGWLEWNGT GL+T+ YGEY+N+GPGA+TS RV W G+SL
Sbjct: 560 WKPYSRTVYIQSYISDVVQPVGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSL 619

Query: 627 MNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLL 653
           +NL QAMNFTVYNFT+GDTWLP TDIPF GGLL
Sbjct: 620 LNLVQAMNFTVYNFTLGDTWLPQTDIPFYGGLL 621

BLAST of MELO3C012316.jh1 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 501.1 bits (1289), Expect = 1.3e-141
Identity = 283/595 (47.56%), Postives = 376/595 (63.19%), Query Frame = 0

Query: 72  IFLLLLSLNFAGASVSPP-SSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKQCI 131
           +FL L +L  A  ++ P  SS+ +CK+T  PK C+S+    + S  D   YG+FS+++ +
Sbjct: 11  LFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVREYGRFSLRKSL 70

Query: 132 KQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFL-RSIESV--LEAAEGV 191
            Q+ K +  I  YL R  ++ L++   GAL DCR L+ L  ++L  S E+V    +++ +
Sbjct: 71  TQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 130

Query: 192 DEELVERVESTLSAIVTNGQTCVDGLVESRSS---LGKALSAPLLSAGELYSVSLGLVSN 251
                + +++ LSA +TN QTC+DG+  + SS   +   ++ PL++  +L+SVSL L   
Sbjct: 131 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLAL--- 190

Query: 252 AMSRRW--KKRREKGGGNGGVPGGGRS-----REALNTLIKGLHKMEPCNNHSTKCSGRK 311
             ++ W  KK+++    +   P    S     R   N  +  L   E         S RK
Sbjct: 191 -FTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALP-LKMTEHTRAVYESLSRRK 250

Query: 312 RLLTDLGSSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEY 371
               D   + +L+++ V V+  G  NFT+I +A+  APN +    GYFVIYV  G YEE 
Sbjct: 251 LADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEEN 310

Query: 372 VVVPKFKTNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATISVRKRYVYDL 431
           VV+ K K  +M+IGDGINRT++TGN NVVDGWTT+NS+TF                    
Sbjct: 311 VVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATF-------------------- 370

Query: 432 RRYDAVCGDGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQDTLYVHSLRQ 491
               AV    FVA+++TFRNTAGPEKHQAVA+R+SADLS FY CSFE YQDTLY HSLRQ
Sbjct: 371 ----AVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQ 430

Query: 492 FYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQNTGISIHNCT 551
           FYRECDIYGTVDFIFGNAA VFQ CNLY R+P+ NQ NA TAQGRTDPNQNTGISIHNCT
Sbjct: 431 FYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCT 490

Query: 552 IKAAPDWVMDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTVGLETLYYG 611
           IK A D ++ SN T   YLGRPWK+YSRTV+MQSYI +++ PVGW EWNG   L TLYY 
Sbjct: 491 IKPADD-LVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYA 550

Query: 612 EYENYGPGANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFSGGLL 653
           EY N G G++T+ RV WPG+ ++N T A NFTV NF +GD W+  + +P+  GLL
Sbjct: 551 EYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of MELO3C012316.jh1 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 469.9 bits (1208), Expect = 3.3e-132
Identity = 271/609 (44.50%), Postives = 367/609 (60.26%), Query Frame = 0

Query: 70  PFIFLLLLSL---NFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSV 129
           P   L+ LS    +   +S +  +S+ +CK+T  PK C+S+      S  +   YG FS+
Sbjct: 4   PIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFSI 63

Query: 130 KQCIKQAIKMSTVIGDYLDRGRDSRLNRREA-GALSDCRELSDLNVEF-LRSIESVLEAA 189
           ++ + Q+ K    +  Y+   R++ L++     AL DCR L+ L +++ L S E+V + +
Sbjct: 64  RKSLSQSRKFIRTVDRYIK--RNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTS 123

Query: 190 -----EGVDEELVERVESTLSAIVTNGQTCVDGLVESRS-----SLGKALSAPLLSAGEL 249
                + +     + +++ LSA +TN QTC++GL  + S     ++   ++ PL++  +L
Sbjct: 124 AKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKL 183

Query: 250 YSVSLGLVSNAMSRRW-KKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKC 309
             VSL L     ++ W  K++++ G     P  G S       +     +       TK 
Sbjct: 184 LGVSLAL----FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKA 243

Query: 310 ---SGRKRLLTDLGSSG-------ILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDG 369
              S  +R L D  S+G       +LI++ V VS  G  NFT+I  A+A APNN+    G
Sbjct: 244 VYESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAG 303

Query: 370 YFVIYVREGYYEEYVVVPKFKTNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDL 429
           +F+IYV  G YEEY+ + K K  +M+IGDGIN+T++TGN +VVDGWTT+NS+TF      
Sbjct: 304 FFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATF------ 363

Query: 430 KATISVRKRYVYDLRRYDAVCGDGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSF 489
                             AV    FVA+++TFRNTAGPEKHQAVALR+ AD S FY CSF
Sbjct: 364 ------------------AVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSF 423

Query: 490 EGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRT 549
           E YQDTLY HSLRQFYRECD+YGTVDFIFGNAA VFQ CNLY RKP+PNQ NA TAQGR+
Sbjct: 424 EAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRS 483

Query: 550 DPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWL 609
           DPNQNTG SI NCTIK A D ++ SN T   YLGRPWK+YSRTVYMQSYI   + PVGW 
Sbjct: 484 DPNQNTGTSIQNCTIKPADD-LVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWR 543

Query: 610 EWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYT 653
           EWNG   L TLYY EY N GPG+NT+ RV WPG+ ++N T A NFTV    +   W+  T
Sbjct: 544 EWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKT 578

BLAST of MELO3C012316.jh1 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 465.7 bits (1197), Expect = 6.2e-131
Identity = 266/604 (44.04%), Postives = 353/604 (58.44%), Query Frame = 0

Query: 66  MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 125
           ++SLP  F +   +   G + SP +   +C+    P  CRS+L +    P D Y YG+ S
Sbjct: 14  LSSLPSPF-ISAQIPAIGNATSPSN---ICRFAPDPSYCRSVLPN---QPGDIYSYGRLS 73

Query: 126 VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 185
           +++ + +A +  ++I   LDR +     +   GAL DC+ L+ L +++L S     ++ +
Sbjct: 74  LRRSLSRARRFISMIDAELDR-KGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTK 133

Query: 186 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 245
            +     E V + LSA +TN QTC++GL  + S  G  LS  L +  +LY VSL L    
Sbjct: 134 TLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENG--LSGDLFNDTKLYGVSLAL---- 193

Query: 246 MSRRWKKRREK---------------GGGNGGVP--GGGRSREALNTLIKGLHKMEPCNN 305
            S+ W  RR++               G  NG +P     R+R   NT+ +          
Sbjct: 194 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTR---------- 253

Query: 306 HSTKCSGRKRLLTDLGSSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIY 365
                  RK L +D  +  + +++ V V   G  NFT+I  AIA APN +   +GYF+IY
Sbjct: 254 -------RKLLQSD--ADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIY 313

Query: 366 VREGYYEEYVVVPKFKTNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFNKNEDLKATIS 425
           V  G YEEYV VPK K  +M+IGDGIN+T+ITGN +VVDGWTT+NS+TF           
Sbjct: 314 VTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATF----------- 373

Query: 426 VRKRYVYDLRRYDAVCGDGFVAIDVTFRNTAGPEKHQAVALRNSADLSTFYRCSFEGYQD 485
                         + G  F+ +++T RNTAGP K QAVALR+  DLS FY CSFE YQD
Sbjct: 374 -------------ILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQD 433

Query: 486 TLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQN 545
           TLY HSLRQFYRECD+YGTVDFIFGNAA V Q CNLY R+P   Q N  TAQGRTDPNQN
Sbjct: 434 TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQN 493

Query: 546 TGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGT 605
           TG +IH CTI+ A D +  SN T   YLGRPWK+YSRTV MQ+YI   + P GW  W+G 
Sbjct: 494 TGTAIHGCTIRPADD-LATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGD 553

Query: 606 VGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLTQAMNFTVYNFTMGDTWLPYTDIPFS 653
             L TLYY EY N GPG++T+ RV WPG+ ++N T A NFTV NF +G+ W+  T +PF 
Sbjct: 554 FALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFV 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008446714.10.095.76PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Cucumis melo][more]
XP_004135375.10.092.36probable pectinesterase/pectinesterase inhibitor 25 [Cucumis sativus] >KGN52152.... [more]
XP_038891461.10.086.78probable pectinesterase/pectinesterase inhibitor 25 [Benincasa hispida][more]
XP_022976564.10.083.33probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima][more]
XP_022150485.10.083.48probable pectinesterase/pectinesterase inhibitor 25 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q94CB18.5e-19460.31Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Q9FF772.0e-18759.51Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3... [more]
Q8RXK71.9e-14047.56Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
Q9SRX44.6e-13144.50Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
O222568.7e-13044.04Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A1S3BFR90.095.76Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103489353 PE=3 SV=1[more]
A0A0A0KUN80.092.36Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G612810 PE=3 SV=1[more]
A0A6J1INW00.083.33Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1[more]
A0A6J1D8L40.083.48Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1[more]
A0A6J1GYC30.082.74Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458594 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10720.26.0e-19560.31Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G04970.11.4e-18859.51Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.11.3e-14147.56Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G02810.13.3e-13244.50Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47550.16.2e-13144.04Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 86..241
e-value: 1.9E-22
score: 90.5
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 71..217
e-value: 1.7E-10
score: 38.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 93..238
e-value: 7.1E-18
score: 65.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 92..245
e-value: 6.0E-23
score: 83.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 93..244
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 310..653
e-value: 1.3E-112
score: 378.2
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 314..400
e-value: 1.1E-22
score: 80.1
coord: 422..637
e-value: 6.7E-107
score: 356.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 251..270
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 422..651
NoneNo IPR availablePANTHERPTHR31707:SF231PECTINESTERASE/PECTINESTERASE INHIBITOR 47-RELATEDcoord: 422..651
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 70..398
NoneNo IPR availablePANTHERPTHR31707:SF231PECTINESTERASE/PECTINESTERASE INHIBITOR 47-RELATEDcoord: 70..398
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 93..244
e-value: 6.34366E-30
score: 113.687
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 484..493
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 309..650

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C012316.jh1.t1MELO3C012316.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity