MELO3C011977 (gene) Melon (DHL92) v4

Overview
NameMELO3C011977
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionPectin lyase-like superfamily protein
Locationchr10: 3474551 .. 3478045 (+)
RNA-Seq ExpressionMELO3C011977
SyntenyMELO3C011977
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACAAATAAGCAAGAAGTTAACTTTTTCACTGTTTCTGTTTTTGGTAGTCTCTTTGCTGGTGTCTTCATCTGTAGTTAGAGTGGAAGCAAGAAAGCAATATCACAAGAAGAGCAAGCATCAGAAGCAAAAGGGTGATGGCAATGGCCATGGTTCAGGCCCTGCACCAGCTCCCCTTCCGTATTACAATCAATCTCAGTCCACCATTTTTAATATCTTGTCTTTTGGAGCAAAGGGCAATGGAGTTTCTGATGATTCCAAGGTAAAAATTGTACAAAACAACCTCAAAAAACAGATACAGAGCTAAAAGGTGTGACTGATATACTTTGTTGAACTTGTGAAAACCATTCTATGCAGGCACTTCTAGCAGCATGGAAAGCTGCATGTGAGATTCCAGGAGCTACGTTGGAAATACCATCACAGCACAAGTTTCTCATCAAACCCATCACTCTAAATGGCCCTTGTATGGCTCAGCTTGTTCTTCAGGTCTGTACAACTTTCTTCTCCTTCAGGATTTTTGTATATTTGAGAATATAACAAAAAAGACAAAAAGTATATTCTTTGACTTGTCATGCAACAGATTGGTTTTTTTTTTTAAAAAAAAGAAATTAGTGCTGTGCTTAGAGTTTAAAGGATAAGATAATTAATGGGAAGTGCAATTCCAAAGCAGATTGATGGAGCTGTATTGGCACCTCCAAAAGTAAGTTCGTGGCCCAAATCCAGCTTGTTTCAATGGCTTAATTTCAAATGGCTACATAACTTCACCGTTCAAGGCTCTGGTACTGTCAATGGCCAGGGCTTTAATTGGTGGACTCTCTCCACATTCTACAATAACAAAGTTAGTTCCTTTTTTCCCCTCTTCGTTAAATTAGGCTAATTACATTGAGAGGTCATGGTTATAGCTAAATAATTTATTGTTGTTTTTGTTTCTGCAGAAGAAATTGAAGCACATTCCAGATATGAAGCCCACTGTAAACTTTTCTTACACTCTCTCTCATTTGCTTGTGATTACGATTTTCGTTTTTAATTCAATTGGGATTTTCTTTTAATACCTTGCTTATAAATGACAGGCTTTGAGATTTTATTCCAGTTATAATGTGACAGTTCGTGACATCACCATAATAAACAGTCCACAGTGCCATCTGAAATTTGACAATTCCGGCGCCGTCAAAATTGACAATATCACCATTTCTTCGCCGGAGAACAGCCCCAACACCGACGGCATTCACCTGCAGAACACTCGAGATGTTGAGATTCAACACTCCAACATTGGCTGTGGTATAAACCGACCCAATTCACAGCCTTTCTTTAATAATATTTTATTGTCCTTTCAAGAGTTAACTTTTACTCTCGATCTTTTTTCTTTCTGTTTTATAATTTTGAGGACACAAGAAAGTGGTGCTTTGAAAAAGAAAATCTTGACTCAAACTTCAAAAATACTTTTTAAGATGTAAAAACAATATAAATGATTAAAATAAAGTAGAAAACTAAAGTTTGATCCGTTTAAATACCCTTCTAATAATATAAACAATTGTTAGAAAAATATTATCAATAAGAATGTTATTATATCTTATTAAAATAATTATTTTAGTTAACAATTTTGTTTGATTGCACTATATTCAAAATTGATTCTATAAATTATTACTATATTTTGAGAATTCTAGGTTAAACATTTTAAAGCCATTTGTTACTAATCATCTCTCAGGTGTTTCTTCAAAGAAGGCTTCTAGATTGATTCAATTTTTTGAACTATTTCTTATTTGTTAAGATTTTTTTTAAATAAAAAAACTACTTTTGATATTCTTAAATCACTGAATAAGCTTTAGTAAACACACTTTTAAATTATTAATTTTTTCAAAGGTGCTTGCAGAAAAGCCAATTTTATTTAAAAAGTATTTATTTTTCTGTTTTCCAAACACACTATTAATTCAGATTGTCACATGTGTAGATTAATGTCAGCCTCAGTTCTAAAGAAATGATCATATAATTTTCTAGGAAAACAAATGTTTTACTCAAATTCACATATCTTCTCTTGTATTTTTAATGATGGAAAATTATGAGGTAAAAATAACTCCATAGAGGTTAGGAAAGTATACCTCCAGTTAACAATATTTTGATTGCAATAGCACATAAAGCTACACAACAAAGATACTGGACTACACAATTGAACCAACTTATTTTAAGATTTGTTATTTTAACAGGAGATGACTGTGTTTCCATTCAGACTGGCTGCTCCAACATCCACATTCATCATATCAATTGTGGCCCTGGACATGGCATAAGGTAAAATTTTAATTTTACTTTTTCCATAAAGCTAATTCCCGTAGAGAATATTATTTATGGGCTTGATTTCGCTCAACTTTTCTTAAACATTATTATATTCAGCACTAAAGATTATTTTCTTGCCTATAATTACAGCTTGGGGGGACTTGGGAAAGATAAAAGTGCTGCATGTGTCTCCAATATTGTTGTCCAAAACATCTCAATTCAAAACACTCTTTCAGGAGTGAGAATCAAGACCTGGCAGGTAAAAAAAAGTTTAAAGTTAAACTACAAATCTGTCTCCTTTAATTATGGTCACTGTTCTATTGTACCTATAATGAATAATTATGGATTGAGTTGATTTCATCATTCAAAAAGAAAAATTATGGATTGATTTGACACGATGAACAAAGTAAGTACATAGATAGAATATAGTACATAGTGACACGGTTTATAAGAAAGATTTCATATGTTCGCTTTGTAAAACGGGAGTATAATACAGTGCATTAGGAAACCCTTTCTTTCTCAATAAAAGCAGTTATTTTATTTTTTTTAAGATGATATATTCAACCATTCTCCTTCACTTGTAATTTATTTCTTGCATTAATGATAGAAATTACATAGAATCACAGATGTAATTCAAACACTCTTATCCCATTTAGCCAATAAAAATCCTGGGATGGATAGAGTCGGACGGTTCTGATTGGATTTGGTCGAATATAAACAGGGAGGAATTGGGTCAGTGAAGAATGTATCATTCTCAAACATCCAAGTTTCTGATGTAAAGGTGCCTATTATGATAGACCAATACTACTGCGACAAGTCCAAATGTAAGAACCAAACGGGAGCAGTTGCAATTTCAGGCATAACATACGACCAAATCATAGGAAGTTACTCAGTACAGCCTATTCATTTAGCCTGCAGCAGCCAAATCCCATGCGTAGACGTTGATCTCATTGACATACAGCTGAAGCCATCTTCAGAATCCAGAGGCTTTCAAAACTCCTTATGTTGGAATTCTTATGGTAAATCTCAAGCCCCTCTTCTTCCTACTAGCATAGACTCTTGCCTTAGAAGGGGTGGTGGTGCTGTAAGGAGAATTGCTAGATCCCATGATTATGTCTGCTACTGATTCTCTATGTTTCCTCTCCTGTTTCTGTTTTTTCCTCTCTCTAGCTTCCCTTCTGCAGTTTTTTCTTTATTTTTTTTGAAAAATGTAGGAAGGTGATGA

mRNA sequence

ATGAAACAAATAAGCAAGAAGTTAACTTTTTCACTGTTTCTGTTTTTGGTAGTCTCTTTGCTGGTGTCTTCATCTGTAGTTAGAGTGGAAGCAAGAAAGCAATATCACAAGAAGAGCAAGCATCAGAAGCAAAAGGGTGATGGCAATGGCCATGGTTCAGGCCCTGCACCAGCTCCCCTTCCGTATTACAATCAATCTCAGTCCACCATTTTTAATATCTTGTCTTTTGGAGCAAAGGGCAATGGAGTTTCTGATGATTCCAAGGCACTTCTAGCAGCATGGAAAGCTGCATGTGAGATTCCAGGAGCTACGTTGGAAATACCATCACAGCACAAGTTTCTCATCAAACCCATCACTCTAAATGGCCCTTGTATGGCTCAGCTTGTTCTTCAGATTGATGGAGCTGTATTGGCACCTCCAAAAGTAAGTTCGTGGCCCAAATCCAGCTTGTTTCAATGGCTTAATTTCAAATGGCTACATAACTTCACCGTTCAAGGCTCTGGTACTGTCAATGGCCAGGGCTTTAATTGGTGGACTCTCTCCACATTCTACAATAACAAAAAGAAATTGAAGCACATTCCAGATATGAAGCCCACTGCTTTGAGATTTTATTCCAGTTATAATGTGACAGTTCGTGACATCACCATAATAAACAGTCCACAGTGCCATCTGAAATTTGACAATTCCGGCGCCGTCAAAATTGACAATATCACCATTTCTTCGCCGGAGAACAGCCCCAACACCGACGGCATTCACCTGCAGAACACTCGAGATGTTGAGATTCAACACTCCAACATTGGCTGTGGAGATGACTGTGTTTCCATTCAGACTGGCTGCTCCAACATCCACATTCATCATATCAATTGTGGCCCTGGACATGGCATAAGCTTGGGGGGACTTGGGAAAGATAAAAGTGCTGCATGTGTCTCCAATATTGTTGTCCAAAACATCTCAATTCAAAACACTCTTTCAGGAGTGAGAATCAAGACCTGGCAGGGAGGAATTGGGTCAGTGAAGAATGTATCATTCTCAAACATCCAAGTTTCTGATGTAAAGGTGCCTATTATGATAGACCAATACTACTGCGACAAGTCCAAATGTAAGAACCAAACGGGAGCAGTTGCAATTTCAGGCATAACATACGACCAAATCATAGGAAGTTACTCAGTACAGCCTATTCATTTAGCCTGCAGCAGCCAAATCCCATGCGTAGACGTTGATCTCATTGACATACAGCTGAAGCCATCTTCAGAATCCAGAGGCTTTCAAAACTCCTTATGTTGGAATTCTTATGGTAAATCTCAAGCCCCTCTTCTTCCTACTAGCATAGACTCTTGCCTTAGAAGGGGTGGTGGTGCTGTAAGGAGAATTGCTAGATCCCATGATTATGTCTGCTACTGATTCTCTATGTTTCCTCTCCTGTTTCTGTTTTTTCCTCTCTCTAGCTTCCCTTCTGCAGTTTTTTCTTTATTTTTTTTGAAAAATGTAGGAAGGTGATGA

Coding sequence (CDS)

ATGAAACAAATAAGCAAGAAGTTAACTTTTTCACTGTTTCTGTTTTTGGTAGTCTCTTTGCTGGTGTCTTCATCTGTAGTTAGAGTGGAAGCAAGAAAGCAATATCACAAGAAGAGCAAGCATCAGAAGCAAAAGGGTGATGGCAATGGCCATGGTTCAGGCCCTGCACCAGCTCCCCTTCCGTATTACAATCAATCTCAGTCCACCATTTTTAATATCTTGTCTTTTGGAGCAAAGGGCAATGGAGTTTCTGATGATTCCAAGGCACTTCTAGCAGCATGGAAAGCTGCATGTGAGATTCCAGGAGCTACGTTGGAAATACCATCACAGCACAAGTTTCTCATCAAACCCATCACTCTAAATGGCCCTTGTATGGCTCAGCTTGTTCTTCAGATTGATGGAGCTGTATTGGCACCTCCAAAAGTAAGTTCGTGGCCCAAATCCAGCTTGTTTCAATGGCTTAATTTCAAATGGCTACATAACTTCACCGTTCAAGGCTCTGGTACTGTCAATGGCCAGGGCTTTAATTGGTGGACTCTCTCCACATTCTACAATAACAAAAAGAAATTGAAGCACATTCCAGATATGAAGCCCACTGCTTTGAGATTTTATTCCAGTTATAATGTGACAGTTCGTGACATCACCATAATAAACAGTCCACAGTGCCATCTGAAATTTGACAATTCCGGCGCCGTCAAAATTGACAATATCACCATTTCTTCGCCGGAGAACAGCCCCAACACCGACGGCATTCACCTGCAGAACACTCGAGATGTTGAGATTCAACACTCCAACATTGGCTGTGGAGATGACTGTGTTTCCATTCAGACTGGCTGCTCCAACATCCACATTCATCATATCAATTGTGGCCCTGGACATGGCATAAGCTTGGGGGGACTTGGGAAAGATAAAAGTGCTGCATGTGTCTCCAATATTGTTGTCCAAAACATCTCAATTCAAAACACTCTTTCAGGAGTGAGAATCAAGACCTGGCAGGGAGGAATTGGGTCAGTGAAGAATGTATCATTCTCAAACATCCAAGTTTCTGATGTAAAGGTGCCTATTATGATAGACCAATACTACTGCGACAAGTCCAAATGTAAGAACCAAACGGGAGCAGTTGCAATTTCAGGCATAACATACGACCAAATCATAGGAAGTTACTCAGTACAGCCTATTCATTTAGCCTGCAGCAGCCAAATCCCATGCGTAGACGTTGATCTCATTGACATACAGCTGAAGCCATCTTCAGAATCCAGAGGCTTTCAAAACTCCTTATGTTGGAATTCTTATGGTAAATCTCAAGCCCCTCTTCTTCCTACTAGCATAGACTCTTGCCTTAGAAGGGGTGGTGGTGCTGTAAGGAGAATTGCTAGATCCCATGATTATGTCTGCTACTGA

Protein sequence

MKQISKKLTFSLFLFLVVSLLVSSSVVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSIDSCLRRGGGAVRRIARSHDYVCY
Homology
BLAST of MELO3C011977 vs. NCBI nr
Match: XP_008446184.2 (PREDICTED: polygalacturonase At1g48100 [Cucumis melo])

HSP 1 Score: 926.0 bits (2392), Expect = 1.3e-265
Identity = 449/450 (99.78%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 17  VVSLLVSSSVVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSF 76
           +VSLLVSSSVVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSF
Sbjct: 1   MVSLLVSSSVVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSF 60

Query: 77  GAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAV 136
           GAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAV
Sbjct: 61  GAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAV 120

Query: 137 LAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDM 196
           LAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDM
Sbjct: 121 LAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDM 180

Query: 197 KPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNT 256
           KPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNT
Sbjct: 181 KPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNT 240

Query: 257 RDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQN 316
           RDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQN
Sbjct: 241 RDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQN 300

Query: 317 ISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAI 376
           ISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAI
Sbjct: 301 ISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAI 360

Query: 377 SGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQA 436
           SGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQA
Sbjct: 361 SGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQA 420

Query: 437 PLLPTSIDSCLRRGGGAVRRIARSHDYVCY 467
           PLLPTSIDSCLRRGGGAVRRIARSHDYVCY
Sbjct: 421 PLLPTSIDSCLRRGGGAVRRIARSHDYVCY 450

BLAST of MELO3C011977 vs. NCBI nr
Match: TYK15622.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 912.9 bits (2358), Expect = 1.1e-261
Identity = 440/441 (99.77%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 26  VVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVSD 85
           +VRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVSD
Sbjct: 3   LVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVSD 62

Query: 86  DSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAVLAPPKVSSW 145
           DSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAVLAPPKVSSW
Sbjct: 63  DSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAVLAPPKVSSW 122

Query: 146 PKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKPTALRFYS 205
           PKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKPTALRFYS
Sbjct: 123 PKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKPTALRFYS 182

Query: 206 SYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHSN 265
           SYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHSN
Sbjct: 183 SYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHSN 242

Query: 266 IGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLSG 325
           IGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLSG
Sbjct: 243 IGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLSG 302

Query: 326 VRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQII 385
           VRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQII
Sbjct: 303 VRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQII 362

Query: 386 GSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSIDS 445
           GSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSIDS
Sbjct: 363 GSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSIDS 422

Query: 446 CLRRGGGAVRRIARSHDYVCY 467
           CLRRGGGAVRRIARSHDYVCY
Sbjct: 423 CLRRGGGAVRRIARSHDYVCY 443

BLAST of MELO3C011977 vs. NCBI nr
Match: KAA0034297.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 874.0 bits (2257), Expect = 5.7e-250
Identity = 429/443 (96.84%), Postives = 431/443 (97.29%), Query Frame = 0

Query: 26  VVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVSD 85
           +VRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVSD
Sbjct: 3   LVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVSD 62

Query: 86  DSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVL-QIDGAVLAPPKVSS 145
           DSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVL QIDGAVLAPPKVSS
Sbjct: 63  DSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQQIDGAVLAPPKVSS 122

Query: 146 WPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKPT-ALRF 205
           WPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNK     +    P  ALRF
Sbjct: 123 WPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNK-----VSSFFPLFALRF 182

Query: 206 YSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQH 265
           YSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQH
Sbjct: 183 YSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQH 242

Query: 266 SNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTL 325
           SNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTL
Sbjct: 243 SNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTL 302

Query: 326 SGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQ 385
           SGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQ
Sbjct: 303 SGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQ 362

Query: 386 IIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSI 445
           IIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSI
Sbjct: 363 IIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSI 422

Query: 446 DSCLRRGGGAVRRIARSHDYVCY 467
           DSCLRRGGGAVRRIARSHDYVCY
Sbjct: 423 DSCLRRGGGAVRRIARSHDYVCY 440

BLAST of MELO3C011977 vs. NCBI nr
Match: XP_023542214.1 (polygalacturonase At1g48100 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 825.5 bits (2131), Expect = 2.3e-235
Identity = 402/456 (88.16%), Postives = 426/456 (93.42%), Query Frame = 0

Query: 12  LFLFLVVSLLVSSSVVRVEARKQYH-KKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTI 71
           L LFL VSLLVSSSVV +EARK +H KKSKHQKQKG   G GS PAPAPLP YNQ+QSTI
Sbjct: 8   LTLFL-VSLLVSSSVVPLEARKHHHNKKSKHQKQKGGAKGSGSSPAPAPLPDYNQTQSTI 67

Query: 72  FNILSFGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVL 131
           FNILSFGAKGNGV DDSKAL+AAWKAAC+IPGATL+IPS +KFLIKPITLNGPCM QLVL
Sbjct: 68  FNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPCMPQLVL 127

Query: 132 QIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKL 191
           QIDG VLAPPKVSSW KSSLFQWLNFKWLHNFT+ GSGTV+GQGFNWWT+ST YNNKKK 
Sbjct: 128 QIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLYNNKKKG 187

Query: 192 KHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDG 251
           KH+PDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSG VK+DNITISSPE+SPNTDG
Sbjct: 188 KHVPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVDNITISSPESSPNTDG 247

Query: 252 IHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVS 311
           IHLQNT+DVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLGGLGKDKSAACVS
Sbjct: 248 IHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGGLGKDKSAACVS 307

Query: 312 NIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQ 371
           NIVV+NISIQNTLSGVRIKTWQGG+GSVKNVSFSNIQVSDVKVPIMIDQYYCDK +CKNQ
Sbjct: 308 NIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYRCKNQ 367

Query: 372 TGAVAISGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNS 431
           TGAVAISGI YDQIIGSY+VQPIHLACSSQIPC+DVDLI IQL PSS SRGFQ++LCWNS
Sbjct: 368 TGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGSRGFQSALCWNS 427

Query: 432 YGKSQAPLLPTSIDSCLRRGGGAVRRIARSHDYVCY 467
           YGKSQAPL+P+SID CLRRG GAVRRIARSHD+ CY
Sbjct: 428 YGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

BLAST of MELO3C011977 vs. NCBI nr
Match: XP_022966725.1 (polygalacturonase At1g48100 [Cucurbita maxima])

HSP 1 Score: 825.1 bits (2130), Expect = 3.1e-235
Identity = 401/456 (87.94%), Postives = 426/456 (93.42%), Query Frame = 0

Query: 12  LFLFLVVSLLVSSSVVRVEARKQYH-KKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTI 71
           L LFL VSLLVSSSVV +EARK +H KKSKHQKQ+G G G GS PAPAPLP YNQ+QSTI
Sbjct: 8   LTLFL-VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDYNQTQSTI 67

Query: 72  FNILSFGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVL 131
           FNILSFGAKGNGV DDSKAL+AAW AAC+IPGATL+IP  +KFLIKPITLNGPCM QLVL
Sbjct: 68  FNILSFGAKGNGVFDDSKALIAAWTAACKIPGATLQIPPDYKFLIKPITLNGPCMPQLVL 127

Query: 132 QIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKL 191
           QIDG VLAPPKVSSW KSSLFQWLNFKWLHNFT+ GSGTV+GQGFNWWT+ST YNNKKK 
Sbjct: 128 QIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLYNNKKKG 187

Query: 192 KHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDG 251
           KH+PDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSG VK+DNITISSPE+SPNTDG
Sbjct: 188 KHVPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGNVKVDNITISSPESSPNTDG 247

Query: 252 IHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVS 311
           IHLQNT+DVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKS ACVS
Sbjct: 248 IHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSVACVS 307

Query: 312 NIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQ 371
           NIVV+NISIQNTLSGVRIKTWQGG+GSVKNVSFSNIQVSDVKVPIMIDQYYCDK +CKNQ
Sbjct: 308 NIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYRCKNQ 367

Query: 372 TGAVAISGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNS 431
           TGAVAISGI YDQIIGSY+VQPIHLACSSQIPC+DVDLI IQL PSS SRGFQ++LCWNS
Sbjct: 368 TGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGSRGFQSALCWNS 427

Query: 432 YGKSQAPLLPTSIDSCLRRGGGAVRRIARSHDYVCY 467
           YGKSQAPL+P+SID CLRRG GAVRRIARSHD+ CY
Sbjct: 428 YGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

BLAST of MELO3C011977 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.3e-92
Identity = 183/419 (43.68%), Postives = 241/419 (57.52%), Query Frame = 0

Query: 53  SGPAPAPLPYYNQSQS-TIFNILSFGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQH 112
           S P P+P P    S S  +F++ SFGA G+G  DD+ A   AWKAAC +    +  P   
Sbjct: 61  SDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGG 120

Query: 113 KFLIKPITLNGPCMAQLVLQIDGAVLAPPKVSSWP-KSSLFQWLNFKWLHNFTVQGSGTV 172
            F I     +GPC   LV Q+DG ++ P     WP K +  QWL F  L  FT  G GTV
Sbjct: 121 VFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTV 180

Query: 173 NGQGFNWWTL--STFYNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDN 232
            G G  WW L               P   PT +RF+ S N+ V+ + I NSPQ H+KFD 
Sbjct: 181 EGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDG 240

Query: 233 SGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHIN 292
              V I+ I ISSP+ SPNTDGIHL NTR V I +S +  GDDC+SI TGCS++ I  + 
Sbjct: 241 CQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVT 300

Query: 293 CGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQV 352
           CGP HGIS+G LG   S ACVSNI V+N  I+++ +G+R+KTWQGG GSV N+ F NIQ+
Sbjct: 301 CGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQM 360

Query: 353 SDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQIIGSYSVQ--PIHLACSSQIPCVDV 412
            +V   I++DQYYC    C+N+T AV +  + Y  I G+Y V+  PIH ACS  + C ++
Sbjct: 361 ENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNI 420

Query: 413 DLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSIDSCLRRGGGAVRRIARSHDYVC 466
            + +++L P  E     +  CWN+YGK +   +P  ID CL  G   V   A   +Y C
Sbjct: 421 TMSEVELLP-EEGELVDDPFCWNAYGKQETLTIP-PID-CLLDGSPVVEE-AYDSNYGC 475

BLAST of MELO3C011977 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 5.1e-84
Identity = 164/385 (42.60%), Postives = 230/385 (59.74%), Query Frame = 0

Query: 65  QSQST------IFNILSFGAKGNGVSDDSKALLAAWKAACEIPGAT-LEIPSQHKFLIKP 124
           QS+ST      + ++ +FGAKGNGV+DD+KA   AWK AC     T + +P  +  L++P
Sbjct: 41  QSRSTRPRSERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRP 100

Query: 125 ITLNGPCMAQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNW 184
           I L+GPC A+L LQI G ++AP     W   +  +WL F  L   TV+G GTVNG G  W
Sbjct: 101 IDLSGPCKARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEW 160

Query: 185 WTLSTFYNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNI 244
           W  S  +N+    +      PTAL F+   N+ V ++ +I+S Q H+   +   V I  +
Sbjct: 161 WERSCKHNHSNPCRG----APTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGL 220

Query: 245 TISSPENSPNTDGIHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISL 304
            + +P  SPNTDGIH+  +R + I ++ +  GDDC+SI    + I I +I CGPGHGIS+
Sbjct: 221 KVIAPATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISI 280

Query: 305 GGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMI 364
           G LGK KS   V +I V    I +T +GVRIKTWQGG G V  + F NI++++V  PI+I
Sbjct: 281 GSLGKSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIII 340

Query: 365 DQYYCDKSK-CKNQTGAVAISGITYDQIIG-SYSVQPIHLACSSQIPCVDVDLIDIQLKP 424
           DQYYCD  K C NQT A++I  I++  + G S S + I ++CS   PC ++ L DI L+P
Sbjct: 341 DQYYCDSRKPCANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEP 400

Query: 425 SSESRGFQNSLCWNSYGKSQAPLLP 441
           S+   GF  S CW +YG S   + P
Sbjct: 401 SN-GDGFTESFCWEAYGSSSGQVYP 420

BLAST of MELO3C011977 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 3.0e-76
Identity = 152/396 (38.38%), Postives = 232/396 (58.59%), Query Frame = 0

Query: 70  IFNILSFGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLV 129
           IFN+  +GA G+G  D ++A   AW+AAC+ P A L +P   KF++  +  NGPC     
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117

Query: 130 LQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKK 189
            ++DG + A    +SW  + +  WL F  L  FT+ G G ++GQG  WW     + N ++
Sbjct: 118 FKVDGIIAAYQNPASWKNNRI--WLQFAKLTGFTLMGKGVIDGQGKQWWAGQCKWVNGRE 177

Query: 190 LKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTD 249
           + +  D +PTA++F  S  + ++ + ++NSP+ HL F N   VKI  I+I++P +SPNTD
Sbjct: 178 ICNDRD-RPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSPNTD 237

Query: 250 GIHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACV 309
           GI +  +++  +Q + IG GDDCV+I TG SNI I  + CGPGHGIS+G LG++ S A V
Sbjct: 238 GIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSRAEV 297

Query: 310 SNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYC-DKSKCK 369
           S + V      +T +G+RIKTWQGG G   ++ + N+++ + + PI+I+Q+YC   S C+
Sbjct: 298 SYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSASACQ 357

Query: 370 NQTGAVAISGITYDQIIG-SYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLC 429
           NQ  AV I  +TY  I G S +   I L CS  +PC D+ L DI LK +S      + L 
Sbjct: 358 NQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKDIKLSDISLKLTSGK--IASCLN 417

Query: 430 WNSYGKSQAPLLPTSIDSCLRRGGGAVRRIARSHDY 464
            N+ G     ++P    +C      A R+ ++SH +
Sbjct: 418 DNANGYFSGHVIP----ACKNLSPSAKRKESKSHKH 444

BLAST of MELO3C011977 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 5.7e-75
Identity = 152/365 (41.64%), Postives = 215/365 (58.90%), Query Frame = 0

Query: 68  STIFNILSFGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQ 127
           S   N+  FGAKG+G  DD+KA   AWKAAC    + + +  +  +L++PI+ +GPC + 
Sbjct: 87  SKTVNVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSG 146

Query: 128 LVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNK 187
           L +QI G + A    S + K     WL F  + N  V+G GT+NG G  WW      N+ 
Sbjct: 147 LTMQIYGTIEASDDRSDYRKDGR-HWLVFDSVQNLRVEGGGTINGNGKIWW-----QNSC 206

Query: 188 KKLKHIP-DMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSP 247
           K  K +P    PTAL FY S +V V+++ I N+ Q H+ FDN   V+  N+ +++PENSP
Sbjct: 207 KTNKALPCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSP 266

Query: 248 NTDGIHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSA 307
           NTDGIH+  T+++ I    IG GDDC+SI  G   + ++ I CGPGHGIS+G LG   S 
Sbjct: 267 NTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSE 326

Query: 308 ACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSK 367
           A VS++VV    +  T +GVRIKTWQGG GS  N+ F N+++ +V+ PI+IDQ YCD+ K
Sbjct: 327 AHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDK 386

Query: 368 -CKNQTGAVAISGITYDQIIGS-YSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQN 427
            C+ Q+ AV +  + Y  I G+  S   I   CS + PC  + L D+ L+   E      
Sbjct: 387 PCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLE--IEGGAAAK 442

Query: 428 SLCWN 430
           +LC N
Sbjct: 447 ALCNN 442

BLAST of MELO3C011977 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 282.7 bits (722), Expect = 7.4e-75
Identity = 146/351 (41.60%), Postives = 214/351 (60.97%), Query Frame = 0

Query: 69  TIFNILSFGAKGNGVSDDSKALLAAWKAACEIPGA-TLEIPSQHKFLIKPITLNGPCMAQ 128
           T  ++  FGAKG+G +DD++A + AWK AC   GA  L +P  + +L+K I L GPC + 
Sbjct: 66  TTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSI 125

Query: 129 LVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTVQG--SGTVNGQGFNWWTLSTFYN 188
           L +QI G + A  K S +    + +W+ F  ++N +V G  +G V+G G  WW      N
Sbjct: 126 LTVQIFGTLSASQKRSDY--KDISKWIMFDGVNNLSVDGGDTGVVDGNGETWW-----QN 185

Query: 189 NKKKLKHIPDMK-PTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPEN 248
           + K+ K  P  K PTAL FY+S ++ V+++ + N+ Q  +  +    V++ N+ +++P +
Sbjct: 186 SCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPAD 245

Query: 249 SPNTDGIHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDK 308
           SPNTDGIH+ NT+++ +  S IG GDDC+SI++G  N+ I+ I CGPGHGIS+G LG D 
Sbjct: 246 SPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDN 305

Query: 309 SAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDK 368
           S A VS + V    +  T +GVRIKT+QGG G+  N+ F NIQ+ +VK PI+IDQ YCDK
Sbjct: 306 SKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDK 365

Query: 369 SKCKNQTGAVAISGITYDQIIG-SYSVQPIHLACSSQIPCVDVDLIDIQLK 415
           SKC  +  AV +  + Y  I G S S   I   CS   PC  + L  + +K
Sbjct: 366 SKCTTEKSAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIK 409

BLAST of MELO3C011977 vs. ExPASy TrEMBL
Match: A0A1S3BEF8 (polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103488989 PE=3 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 6.2e-266
Identity = 449/450 (99.78%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 17  VVSLLVSSSVVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSF 76
           +VSLLVSSSVVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSF
Sbjct: 1   MVSLLVSSSVVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSF 60

Query: 77  GAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAV 136
           GAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAV
Sbjct: 61  GAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAV 120

Query: 137 LAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDM 196
           LAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDM
Sbjct: 121 LAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDM 180

Query: 197 KPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNT 256
           KPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNT
Sbjct: 181 KPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNT 240

Query: 257 RDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQN 316
           RDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQN
Sbjct: 241 RDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQN 300

Query: 317 ISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAI 376
           ISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAI
Sbjct: 301 ISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAI 360

Query: 377 SGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQA 436
           SGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQA
Sbjct: 361 SGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQA 420

Query: 437 PLLPTSIDSCLRRGGGAVRRIARSHDYVCY 467
           PLLPTSIDSCLRRGGGAVRRIARSHDYVCY
Sbjct: 421 PLLPTSIDSCLRRGGGAVRRIARSHDYVCY 450

BLAST of MELO3C011977 vs. ExPASy TrEMBL
Match: A0A5D3CVS9 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001190 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 5.4e-262
Identity = 440/441 (99.77%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 26  VVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVSD 85
           +VRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVSD
Sbjct: 3   LVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVSD 62

Query: 86  DSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAVLAPPKVSSW 145
           DSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAVLAPPKVSSW
Sbjct: 63  DSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAVLAPPKVSSW 122

Query: 146 PKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKPTALRFYS 205
           PKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKPTALRFYS
Sbjct: 123 PKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKPTALRFYS 182

Query: 206 SYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHSN 265
           SYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHSN
Sbjct: 183 SYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHSN 242

Query: 266 IGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLSG 325
           IGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLSG
Sbjct: 243 IGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLSG 302

Query: 326 VRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQII 385
           VRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQII
Sbjct: 303 VRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQII 362

Query: 386 GSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSIDS 445
           GSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSIDS
Sbjct: 363 GSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSIDS 422

Query: 446 CLRRGGGAVRRIARSHDYVCY 467
           CLRRGGGAVRRIARSHDYVCY
Sbjct: 423 CLRRGGGAVRRIARSHDYVCY 443

BLAST of MELO3C011977 vs. ExPASy TrEMBL
Match: A0A5A7SYE1 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G002970 PE=3 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 2.8e-250
Identity = 429/443 (96.84%), Postives = 431/443 (97.29%), Query Frame = 0

Query: 26  VVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVSD 85
           +VRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVSD
Sbjct: 3   LVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGAKGNGVSD 62

Query: 86  DSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVL-QIDGAVLAPPKVSS 145
           DSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVL QIDGAVLAPPKVSS
Sbjct: 63  DSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQQIDGAVLAPPKVSS 122

Query: 146 WPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKPT-ALRF 205
           WPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNK     +    P  ALRF
Sbjct: 123 WPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNK-----VSSFFPLFALRF 182

Query: 206 YSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQH 265
           YSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQH
Sbjct: 183 YSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQH 242

Query: 266 SNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTL 325
           SNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTL
Sbjct: 243 SNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTL 302

Query: 326 SGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQ 385
           SGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQ
Sbjct: 303 SGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQ 362

Query: 386 IIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSI 445
           IIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSI
Sbjct: 363 IIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSI 422

Query: 446 DSCLRRGGGAVRRIARSHDYVCY 467
           DSCLRRGGGAVRRIARSHDYVCY
Sbjct: 423 DSCLRRGGGAVRRIARSHDYVCY 440

BLAST of MELO3C011977 vs. ExPASy TrEMBL
Match: A0A6J1HSE8 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111466350 PE=3 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.5e-235
Identity = 401/456 (87.94%), Postives = 426/456 (93.42%), Query Frame = 0

Query: 12  LFLFLVVSLLVSSSVVRVEARKQYH-KKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTI 71
           L LFL VSLLVSSSVV +EARK +H KKSKHQKQ+G G G GS PAPAPLP YNQ+QSTI
Sbjct: 8   LTLFL-VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDYNQTQSTI 67

Query: 72  FNILSFGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVL 131
           FNILSFGAKGNGV DDSKAL+AAW AAC+IPGATL+IP  +KFLIKPITLNGPCM QLVL
Sbjct: 68  FNILSFGAKGNGVFDDSKALIAAWTAACKIPGATLQIPPDYKFLIKPITLNGPCMPQLVL 127

Query: 132 QIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKL 191
           QIDG VLAPPKVSSW KSSLFQWLNFKWLHNFT+ GSGTV+GQGFNWWT+ST YNNKKK 
Sbjct: 128 QIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLYNNKKKG 187

Query: 192 KHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDG 251
           KH+PDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSG VK+DNITISSPE+SPNTDG
Sbjct: 188 KHVPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGNVKVDNITISSPESSPNTDG 247

Query: 252 IHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVS 311
           IHLQNT+DVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKS ACVS
Sbjct: 248 IHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSVACVS 307

Query: 312 NIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQ 371
           NIVV+NISIQNTLSGVRIKTWQGG+GSVKNVSFSNIQVSDVKVPIMIDQYYCDK +CKNQ
Sbjct: 308 NIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYRCKNQ 367

Query: 372 TGAVAISGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNS 431
           TGAVAISGI YDQIIGSY+VQPIHLACSSQIPC+DVDLI IQL PSS SRGFQ++LCWNS
Sbjct: 368 TGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGSRGFQSALCWNS 427

Query: 432 YGKSQAPLLPTSIDSCLRRGGGAVRRIARSHDYVCY 467
           YGKSQAPL+P+SID CLRRG GAVRRIARSHD+ CY
Sbjct: 428 YGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

BLAST of MELO3C011977 vs. ExPASy TrEMBL
Match: A0A6J1FZF6 (polygalacturonase At1g48100 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 1.6e-234
Identity = 400/456 (87.72%), Postives = 425/456 (93.20%), Query Frame = 0

Query: 12  LFLFLVVSLLVSSSVVRVEARKQYH-KKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTI 71
           L LFL VSLLVSSSVV +EARK +H KKSKHQKQ+G G G GS PAPAPLP YNQ+QSTI
Sbjct: 8   LTLFL-VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDYNQTQSTI 67

Query: 72  FNILSFGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVL 131
           FNILSFGAKGNGV DDSKAL+AAWKAAC+IPGATL+IPS +KFLIKPITLNGPCM QLVL
Sbjct: 68  FNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPCMPQLVL 127

Query: 132 QIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKL 191
           QIDG VLAPPKVSSW KSSLFQWLNFKWLHNFT+ G GTV+GQGFNWWT+ST YNNKKK 
Sbjct: 128 QIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWTVSTLYNNKKKG 187

Query: 192 KHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDG 251
           KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSG VK++NITISSPE+SPNTDG
Sbjct: 188 KHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITISSPESSPNTDG 247

Query: 252 IHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVS 311
           IHLQNT+DVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLGGLGKDKS ACVS
Sbjct: 248 IHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGGLGKDKSVACVS 307

Query: 312 NIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQ 371
           NIVV+NISIQNTLSGVRIKTWQGG+GSVKNVSFSNIQVSDVKVPIMIDQYYCDK +CKNQ
Sbjct: 308 NIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYRCKNQ 367

Query: 372 TGAVAISGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNS 431
           TGAVAISGI YDQIIGSY+VQPIHLACSSQIPC+DVDLI IQL PSS SRGFQ++LCWNS
Sbjct: 368 TGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGSRGFQSALCWNS 427

Query: 432 YGKSQAPLLPTSIDSCLRRGGGAVRRIARSHDYVCY 467
           YGKSQAPL+P+SID CLRRG GAVRRIARSHD+ CY
Sbjct: 428 YGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

BLAST of MELO3C011977 vs. TAIR 10
Match: AT3G26610.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 624.0 bits (1608), Expect = 9.6e-179
Identity = 297/468 (63.46%), Postives = 373/468 (79.70%), Query Frame = 0

Query: 6   KKLTFSLFLFLVVSLLVSSSVVRVEARKQYHKKSKHQKQKGDGNGHGSGPAPAPLPYYNQ 65
           K L     L  V+ +  + S V VE RK + KK K + ++   N     PAPAP P  N+
Sbjct: 5   KSLPILAMLLGVIIVAAAISTVSVEGRKHHVKKIKPKHRRHSKNTPTGSPAPAPYPSTNE 64

Query: 66  SQSTIFNILSFGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCM 125
               +FNI S+GAKG+GVSDDSKAL+ AWKAAC++ G  +EIP+  +FL+K +TL GPC 
Sbjct: 65  G---VFNIFSYGAKGDGVSDDSKALVGAWKAACKVVGGKVEIPAGTQFLVKAVTLQGPCK 124

Query: 126 AQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYN 185
            + V+QI+G ++AP K+ SWP SSLFQWLNFKW+ + T+QGSGT+NG+G+NWW L T Y 
Sbjct: 125 EETVVQIEGILVAPEKIGSWPNSSLFQWLNFKWVSHLTIQGSGTLNGRGYNWWNLDT-YQ 184

Query: 186 NKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENS 245
            + + K+IP MKPTALRFYSS NVTVRDI+I+NSP CHLKFD+S  VK++NITISSPENS
Sbjct: 185 TQTRNKYIPPMKPTALRFYSSNNVTVRDISIVNSPLCHLKFDDSDGVKVNNITISSPENS 244

Query: 246 PNTDGIHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKS 305
           PNTDGIHLQNTR+VEIQHSNI CGDDCVSIQTG SN+HIHHINCGPGHGIS+GGLGKDKS
Sbjct: 245 PNTDGIHLQNTRNVEIQHSNIACGDDCVSIQTGSSNVHIHHINCGPGHGISIGGLGKDKS 304

Query: 306 AACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKS 365
            ACVS+I+V++ISIQNTL+GVRIKTWQGG+G VKN++FSNIQV DVKVPI+IDQYYCDKS
Sbjct: 305 VACVSDIIVEDISIQNTLAGVRIKTWQGGLGVVKNLTFSNIQVKDVKVPIVIDQYYCDKS 364

Query: 366 KCKNQTGAVAISGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGF--- 425
           KCKNQT AV+ISG+ Y+ I+GS++VQP+ +ACS+ +PC+DVDL+DI+L+PS   RG    
Sbjct: 365 KCKNQTRAVSISGVKYNNIVGSFTVQPVRIACSNNVPCMDVDLMDIRLRPSGGIRGLQTH 424

Query: 426 --QNSLCWNSYGKSQAPLLPTSIDSCLRR---GGGAVRRIARSHDYVC 466
             Q +LCWNSYGK+Q PL+P+SI  CLR+   GG   ++++RS+D +C
Sbjct: 425 QQQQALCWNSYGKTQGPLVPSSIGYCLRKSNIGGYYSQKVSRSYDKIC 468

BLAST of MELO3C011977 vs. TAIR 10
Match: AT1G10640.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 439.1 bits (1128), Expect = 4.4e-123
Identity = 222/466 (47.64%), Postives = 303/466 (65.02%), Query Frame = 0

Query: 35  YHKKSKHQ---KQKGDGNGHG------SGP---APAPLPYYNQSQSTIFNILSFGAKGNG 94
           +H KSK +   K K     H       SGP    P PLP   Q  S +FN++ FGAKG+G
Sbjct: 73  HHHKSKPKPKPKLKTPPKAHDNNSPVVSGPPKVQPPPLP--PQKGSQVFNVMDFGAKGDG 132

Query: 95  VSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGP-CMAQLVLQIDGAVLAPPK 154
             DD+KA  AAW AAC+I  + + +P ++ +L+ PI+ +GP C A +V Q+DG ++AP  
Sbjct: 133 KCDDTKAFEAAWVAACKIEASVMLVPPEYTYLVGPISFSGPYCQANIVFQLDGTIIAPTD 192

Query: 155 VSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTL-STFYNNKKKL---------- 214
             +W K  L  W++F  L    VQG G ++G+G  WW   S F ++  KL          
Sbjct: 193 SKTWGK-GLMWWIDFTKLKGIKVQGKGVIDGRGSGWWQQDSPFIDSDTKLIVPLNNSANQ 252

Query: 215 -----------KHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITI 274
                      + +P +KPTALRF  S+ V V  ITI NSPQCHLKFD+   V + +I +
Sbjct: 253 NPPMPIRSELDERMPSIKPTALRFSGSFGVEVTGITIQNSPQCHLKFDDCVGVVVHDIAV 312

Query: 275 SSPENSPNTDGIHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGG 334
           SSP +SPNTDGIHLQNT+DV I  + + CGDDC+SIQTGCSN+ +H++NCGPGHGIS+G 
Sbjct: 313 SSPGDSPNTDGIHLQNTKDVLIHSTTLACGDDCISIQTGCSNVFVHNVNCGPGHGISIGS 372

Query: 335 LGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQ 394
           LGK+ + ACVSNI V+++++ NT++GVRIKTWQGG+GSVK + FSNIQ++ V++PI I+Q
Sbjct: 373 LGKEGTKACVSNITVRDVAMHNTMTGVRIKTWQGGVGSVKGIIFSNIQLNQVQIPITINQ 432

Query: 395 YYCDKSKCKNQTGAVAISGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSES 454
           +YCD SKCKNQT AVA+ G+TY++I G+Y+V+P+H ACS   PCVDV L  I+LKP  E 
Sbjct: 433 FYCDHSKCKNQTSAVAVEGVTYERIKGTYTVKPVHFACSDNFPCVDVQLSSIELKPVQEK 492

Query: 455 RGFQNSLCWNSYGKSQAPLLPTSIDSCLRRGGGAVRRIARSHDYVC 466
               ++ CW ++G+   P LP  ID CL+ G     ++   HD VC
Sbjct: 493 YRMYDAYCWQTFGELNTPTLP-PID-CLKIGKPPRNKVQSDHD-VC 532

BLAST of MELO3C011977 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 439.1 bits (1128), Expect = 4.4e-123
Identity = 211/434 (48.62%), Postives = 290/434 (66.82%), Query Frame = 0

Query: 55  PAPAPLPYYNQSQSTIFNILSFGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFL 114
           P P PLP      S  FN+L FGAKG+G+SDD++A  AAW +AC++  +T+ IP  + FL
Sbjct: 107 PPPTPLPLQPVEDSQQFNVLDFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFL 166

Query: 115 IKPITLNGP-CMAQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQ 174
           + PI+ +GP C A +V Q++G ++AP    SW    L  W+ F  L   T+QG+G ++G+
Sbjct: 167 VGPISFSGPYCQANIVFQLEGMIVAPTDTESW-GGGLMWWIEFTKLSGITIQGNGVIDGR 226

Query: 175 GFNWWT---LSTF-----------YNNKKKLK-----------HIPDMKPTALRFYSSYN 234
           G  WW    LS +            NN  + +            +P +KPTALRFY S +
Sbjct: 227 GTVWWQQDYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSID 286

Query: 235 VTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHSNIGC 294
           VTV  ITI NSPQCHLKFDN   V + ++ +SSP +SPNTDGIHLQNTRDV I  + + C
Sbjct: 287 VTVTGITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLAC 346

Query: 295 GDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLSGVRI 354
           GDDC+SIQTGCSN+++H++NCGPGHGIS+G LGKD + ACVSNI V+++ + NT++GVRI
Sbjct: 347 GDDCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRI 406

Query: 355 KTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQIIGSY 414
           KTWQGGIGSVK + FSNIQ+++V++PI+IDQ+YCD SKC N T AV++ G+TY++I G+Y
Sbjct: 407 KTWQGGIGSVKGILFSNIQLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTY 466

Query: 415 SVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLPTSIDSCLR 463
           +V+P+H ACS   PC+DV L  I+LKP        +  CW ++G+  +  +P  ID CL+
Sbjct: 467 TVKPVHFACSDSFPCIDVQLSGIELKPVQLQYHMYDPFCWKTFGELNSATVP-PID-CLQ 526

BLAST of MELO3C011977 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.7e-111
Identity = 189/374 (50.53%), Postives = 254/374 (67.91%), Query Frame = 0

Query: 68  STIFNILSFGAKGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNG-PCMA 127
           S   N+L  GAKG+G SDD+KA   AW+ AC++  +TL +PS   FL+ P++  G  C  
Sbjct: 48  SGTINVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGKECKE 107

Query: 128 QLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNN 187
           ++V Q++G ++AP   S+W  S L QW+ FK L   T++G G ++G+G  WW       N
Sbjct: 108 KIVFQLEGKIIAPTSASAW-GSGLLQWIEFKALQGITIKGKGIIDGRGSVWW-------N 167

Query: 188 KKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSP 247
                 +P  KPTALRFY S  VTV  ITI NSPQ HLKFDN  ++++ + T SSP +SP
Sbjct: 168 DMMGTKMPRTKPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDFTTSSPGDSP 227

Query: 248 NTDGIHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSA 307
           NTDGIHLQN++D  I  S + CGDDC+SIQTGCSNI+IH ++CGPGHGIS+GGLGKD + 
Sbjct: 228 NTDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISIGGLGKDNTK 287

Query: 308 ACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSK 367
           ACVSNI V+++++  T +GVRIK+WQGG GSVK V FSNIQVS+V  PI+IDQYYCD   
Sbjct: 288 ACVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPIIIDQYYCDGGG 347

Query: 368 CKNQTGAVAISGITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSL 427
           C N+T AVA+S I Y  I G+Y+ +P+  ACS  +PC  + L  I+LKP++      +  
Sbjct: 348 CHNETSAVAVSNINYINIKGTYTKEPVRFACSDSLPCTGISLSTIELKPATGKASSLDPF 407

Query: 428 CWNSYGKSQAPLLP 441
           CW ++G+ +   LP
Sbjct: 408 CWKAHGELKTKTLP 413

BLAST of MELO3C011977 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 358.6 bits (919), Expect = 7.5e-99
Identity = 178/396 (44.95%), Postives = 247/396 (62.37%), Query Frame = 0

Query: 55  PAPAPLPYYNQSQS---TIFNILSFGAKGNGVSDDSKALLAAWKAACEIPG---ATLEIP 114
           P+PAP P YN + +   T+F++ SFGA G+  +DD+ A   AW AAC   G   A L +P
Sbjct: 29  PSPAPNPAYNDNDNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALLLVP 88

Query: 115 SQHKFLIKPITLNGPCMAQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTVQGSG 174
               FL+KP T NGPC   LVLQIDG +++P    SWP +   QW+ F  ++  ++QGSG
Sbjct: 89  YTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSNYQRQWMMFYRVNGLSIQGSG 148

Query: 175 TVNGQGFNWWTL--STFYNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKF 234
            +NG+G  WW L            +  P   P A+R + S  V ++ I  +NS Q H++F
Sbjct: 149 VINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQFHVRF 208

Query: 235 DNSGAVKIDNITISSPENSPNTDGIHLQNTRDVEIQHSNIGCGDDCVSIQTGCSNIHIHH 294
           DN   V +D++ I +P +SPNTDGIH++NT +V+I++S I  GDDC+SI  GC N+ I +
Sbjct: 209 DNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNVDIKN 268

Query: 295 INCGPGHGISLGGLGKDKSAACVSNIVVQNISIQNTLSGVRIKTWQGGIGSVKNVSFSNI 354
           + CGP HGIS+G LG   S A VSNI V N +I N+ +GVRIKTWQGG GSV  + FSNI
Sbjct: 269 VTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIVFSNI 328

Query: 355 QVSDVKVPIMIDQYYCDKSKCKNQTGAVAISGITYDQIIGSYSVQ--PIHLACSSQIPCV 414
            + +V+ PIMIDQYYC  + C NQT AV IS + Y  I G+Y ++  PIH  CS  +PC 
Sbjct: 329 LMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDSVPCT 388

Query: 415 DVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPLLP 441
           ++ L ++ L P S+ +  +N  CWN+YG  +  + P
Sbjct: 389 NLTLTEVDLFP-SKGQHLENPFCWNAYGSMKITVPP 423

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008446184.21.3e-26599.78PREDICTED: polygalacturonase At1g48100 [Cucumis melo][more]
TYK15622.11.1e-26199.77polygalacturonase [Cucumis melo var. makuwa][more]
KAA0034297.15.7e-25096.84polygalacturonase [Cucumis melo var. makuwa][more]
XP_023542214.12.3e-23588.16polygalacturonase At1g48100 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022966725.13.1e-23587.94polygalacturonase At1g48100 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q949Z11.3e-9243.68Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ55.1e-8442.60Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432123.0e-7638.38Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
P353365.7e-7541.64Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Q8RY297.4e-7541.60Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3BEF86.2e-26699.78polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103488989 PE=3 SV=1[more]
A0A5D3CVS95.4e-26299.77Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001... [more]
A0A5A7SYE12.8e-25096.84Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G002... [more]
A0A6J1HSE81.5e-23587.94polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111466350 PE=3 SV=... [more]
A0A6J1FZF61.6e-23487.72polygalacturonase At1g48100 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
Match NameE-valueIdentityDescription
AT3G26610.19.6e-17963.46Pectin lyase-like superfamily protein [more]
AT1G10640.14.4e-12347.64Pectin lyase-like superfamily protein [more]
AT1G60590.14.4e-12348.62Pectin lyase-like superfamily protein [more]
AT5G14650.11.7e-11150.53Pectin lyase-like superfamily protein [more]
AT1G56710.17.5e-9944.95Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 256..277
e-value: 4000.0
score: 2.9
coord: 206..255
e-value: 61.0
score: 12.5
coord: 279..299
e-value: 7800.0
score: 0.9
coord: 309..330
e-value: 42.0
score: 13.0
coord: 338..359
e-value: 2400.0
score: 4.5
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 103..420
e-value: 3.4E-81
score: 272.8
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 57..446
e-value: 9.1E-116
score: 389.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..58
NoneNo IPR availablePANTHERPTHR31375:SF92PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 25..453
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 25..453
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 60..442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C011977.1MELO3C011977.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity