MELO3C011342.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C011342.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
DescriptionPseudouridine synthase
Locationchr03: 25249672 .. 25254400 (+)
RNA-Seq ExpressionMELO3C011342.jh1
SyntenyMELO3C011342.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonstart_codonCDSpolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAGAAAACAGACAGCCGATTAGCGAAAACCCAAAAACGAACATATCGAAGACGGGAGAATGATTTCCGTTTGCTCCGCTCGAACAATTCAAGCTTCACCTTCTTCTATTCACTTCTTTTTCCCTCGAAATCGTAGGAGAATCGATTCAAATGTTCTTCGATTTTCCGCCAATTTCTCCAGTAGATGCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCCGGAACCAGACTGGAAGAGACCGTGGACATCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATATCTTCCCGCATTAACGGTATTAGTAGGGCTCGAGTTCAGTCCAGTATCAGAGCGGGATTAGTTTCAGTTAATGGCCGAGTTATCGATAAGGTAGCGTCATTTTTCCATAGAGAAGCTACATTTGCTTAACATTTTTTTCCTTTCTATTTAGCTTCTTTCAGCTATTTTAGTGTGATTATCAAGTGGTCTGTAATCTGGATGGCGAATTATGTTCAGCCGATATTTTCTGTTTTGTTCTATATTTGAGTTCTTAACTTGTGTGTTCACTTGCAACTTCTATATATTTTTTTAATACTCAAGCAGGTTTCTCACAATGTAAAAGCTGGGGATGTGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCTCACATGGTAATGATTCAAAAAAGTTTAATATTGTTATTAGCAATACTATTGGAAGTTCTTTGTGGATGCAAGATACCGATACTTGTGGATAAAATTAAACGCTCTCCCCTCCCCCGCCTGAAGTTTTTAGTAAGATTGAAATTTGCACGTATCTGTGTTTAGCGGTTTTCTTCATGATTCAGGTTGTTCATCCAGCACCTGGAAATGCGACGGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCGATGGTTTCAACATCAACTGGGGAACATCAGTCGGATTCGGATGTTTCAGATGATGAGTTCAGTTCAATTGAAGTTTTTTCTAGAGTTTCTGAGGCGCCTATTCGTCCCGGTATTGTGCATAGGTTAGATAAAGGAACTAGTGGGTTGCTTGTTGTTGCCAAGGTGATTTGCTCTCCTTTTTAAGCTTCTTTGTTTCTGAAGATTAAAGCAGACAATTCAAATTTGGCGCTTCAAAAGTTTGCTACTCAAACACTTGTAATATCATGTCTAACTTCCAAGTTTTTTGCATTGGAACTACAGGATGAACATTCCCATGCTCACTTATCTGCTCAATTCAAGCAACATTCTATTGAGAGAGTTTATATTAGTCTCACATGTGGAGTTCCACCCTCAACTGCTGGGCGTATCGAGATACCAATTTGCCGTGATCCAAACAATCGTATCCGCATGACTGCAACACATGGAACAAAAAATAATCCACATGCAAAGCACGCTGCTAGTAGGTAATTAGTTTTTCTGCATCTTTTATTTTATTTTATGTACATAATTATTTTACAGTGATAGAAAAAATTTAAATATCATTAATTTTGGGCGTTCCTAATGCAAAGTTGAAACACTGAACGTTTCACAGATACAATGTTATGGAAATTTTAGCCCAAGGAGGTTCTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACGCATCAGGTACGTAGTGTAAATGTCTTTCTCCTTAGGCTGCTTCAAATGACAATATATATATACATATTACAATCCATCAACAATGCCAGTTTAGAACATAAATTATATTCCCTAAACACCACGTATATGTGCAAAGACATACATTTCTTTTGTTTGTAAAATGTTTCTCTACAATACTGCTACTGTTTTCCAGTCTTACGTATATATGCATATGTAACTCTTGAACATACAAGCAGCCACATTTACCATTCCACATTCGCTGCAATTTCAGTTCTTTTCTTTCTTTTTTTCTTTTGAAAATTGAAATGCAGTTTTACTTTACTCGGTCTTAGGATTGAATATTTGTGAAGTAGATACGTGCACATGCGAAGTATATGGGAATACCGTTACTTGGAGACGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGAACTAGAGCATCATCTAATTGTCACGGCCAACTCATGCAGCTTGTTTCTTCATTAGAGAGGCCCTGTCTTCATGCTCTGACTCTTGGGTTAGAAAATAAATCTTTAAGAGCTCATTGAGTTACATTCTTATTTCATTTACTATCCGTTTTTCGAGAAATATGTGATTTTGACCTTTCTTCTTTATTTTATTTTATTTTATTTTATTTTATTTTTTCTTTTCTAAAATAGTTTATAATTTTCATCTCTTATTTCTAATCTTCTGAAACTAAATGGGATTTCTATTTATATTATTTTCCTTTAATAATTGTATGCAAAATATCAAAACATTAATTTGACATATACTCATTTCCAATTTTCAGTTAAAATTTCCACTTTTTGAAATAGAAACGTTGTAGAGAAAAATCAACGATAACGATTGCTAATAACATTTTTGTTCTTTTTTAATAAAAATGGAGAAAAGCAAGAAATGGTATAGAAACATGTTTTCACTTTTTACAATAAAAGAGACTGCAATCAAGTGAGAAGCTAGAATAAGATCATTAATAATGAATCTTTACTTGTTTATTTCCTCCATAAAAAACTAATTCAAGATGACTGAATATTTTGTAGGTTTGTACATCCTCACACGGGGAAGAACATACGTTTTTCATGCCCACCCCCAACAGATTTTGCTGAAATTTTGAGTCAGCTTCGTGAAATTGGCACAAAAAAGGTATCCCTTATTTTGATTTCTTTCATTTTATAAGTCGAGCCAAGCCCTTTGCTTGGTAATTGGTTATTTTACAGATTTAATATCCATCCATCATCATGAAGAACAATATAAAATATGGCCCAAGGGCTACAATTGACACAACCAACCTCATTCGTAGCTACCTAGATAGTTAATACGTGTCACCTTTCTGCCTATTTTCTGGGCGCCCCCTTGGCATGTTATAGCTAATACCAGGTCTTCTCTATTTACTAGCTATGTTTCACCTGGTTCTTGCGTTTGGAGTGACTATTTATTGGTTTACTTTCCTTTTGTACTTAATCGCTAACTGATTCTACTAAACTTAAAATAAATATAAATTGTGTAATGTGGGTGAAAATTTAGCAATACATAAAATATATTTTCTAATTATTTAAACGATGTTTTTATACGATGAAACATTGGAGACATGATAGATATAACATAATAACTAAATAGCAGACAAACAAGCATAGAAGTTCTCCTTTTACTTGCTTCTCTTATAAATAGTAACGAATATGGACCTTTGTTCTATCCGTTCCAGATTATCTATACAAATGACATAATGGGGACAAATCCCTGAACAATACCGTGAACTTATACCATGTGGAAATTTCGCATCATATCTTGTGGATTAATCTTGTGCAGGTCTAATGGTAGATAGGGCAAATTGAAATAATTTTGAGCTTTACAAAATATATTAGATGTTGAACAATGAATACTTCCATAGATACGATGTCCCAGTTAACCTACAATAGAGTGTTGTCATAAAAGTTGCAGGTTACCAAATCTTAGCTGGGAGCCTCCAGTATCTGGCGAGTAGACTCATCGTCAAATGGCTCTAAAATGAAAGATCAAAAAGGTACATTAAGCCCTAACATACAGCCATTTAAGTTATGATGTCTGAAGCACAAATTTAAGTTCTGGGTAACTTACATATCTTGAATTCCATTTACAAATTTGACGTTGAATTCCACGTATTTTTAATCTCTGCCATCAACTTCGTTTATTGAAACCATTTAGAATCAATATTGTAAATACGGTTAGTTACTAAAGTAGTTAGCTATATACAGTTAATTCTAATACATTTCTTAAAATTTAGATGAAGAAACACAATTGGATTCTTTCTTCTGGCCACCGCTTCTTTTATTTCATTTTCCATATTCAGTGACCTTTCCCCGATCTTTTCGCTTCAATTCATAATAAGAATATGTTGATGTTTGGGTTAGTGTGTTTTGCTCTGTTACTTTCCTTCGAATATTCAACTCTGTTAATTTGCTTACAATAATATACACAGGAAATGCTCCGATTGCTCAATAACTCGTATTGAAACTGAGATTTAGCAGCATGCAACAAGAGCATAAGGTCAGCAGAAACCCCACACGATATCGGTCCTCCCCACGGTGTTTCTTCTATCCAAACTATCATGTAAGGTATCTGATATTGGTAATGATATGGTATTCGTAAGGGCACAAGGGTAGTTAGTTAAAAGTTTGCGTAGTTGGTTATAAATAGAGGGGAGCATACTTTCCCTCAGTCCCCCAATTCTCCCCTATTTGTAACCAACTACGCTAATTTCCTGGCTAACTACGCCAACGCCCTTACTAATACAATACTAATATCCGGACCTTAAATAGGTACTTTATCGTTCGTTGCTTGTGTCGAACATTTTCCTACTCTCCATGTGCAATGGAACCTCATGTTCAACTTAATGTTGATGGTAAGCCACCATTCCTATTAAACATTCGATTCCATTGCAATCTTCTTTTTCGGTTTCATATGATTTAAATAATATGTTTCGAAAGATGACAAAATTGAATGATTATTATTTTTTTAGATTTCTAATAAAAGTCA

mRNA sequence

GTAAGAAAACAGACAGCCGATTAGCGAAAACCCAAAAACGAACATATCGAAGACGGGAGAATGATTTCCGTTTGCTCCGCTCGAACAATTCAAGCTTCACCTTCTTCTATTCACTTCTTTTTCCCTCGAAATCGTAGGAGAATCGATTCAAATGTTCTTCGATTTTCCGCCAATTTCTCCAGTAGATGCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCCGGAACCAGACTGGAAGAGACCGTGGACATCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATATCTTCCCGCATTAACGGTATTAGTAGGGCTCGAGTTCAGTCCAGTATCAGAGCGGGATTAGTTTCAGTTAATGGCCGAGTTATCGATAAGGTTTCTCACAATGTAAAAGCTGGGGATGTGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCTCACATGGTTGTTCATCCAGCACCTGGAAATGCGACGGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCGATGGTTTCAACATCAACTGGGGAACATCAGTCGGATTCGGATGTTTCAGATGATGAGTTCAGTTCAATTGAAGTTTTTTCTAGAGTTTCTGAGGCGCCTATTCGTCCCGGTATTGTGCATAGGTTAGATAAAGGAACTAGTGGGTTGCTTGTTGTTGCCAAGGATGAACATTCCCATGCTCACTTATCTGCTCAATTCAAGCAACATTCTATTGAGAGAGTTTATATTAGTCTCACATGTGGAGTTCCACCCTCAACTGCTGGGCGTATCGAGATACCAATTTGCCGTGATCCAAACAATCGTATCCGCATGACTGCAACACATGGAACAAAAAATAATCCACATGCAAAGCACGCTGCTAGTAGATACAATGTTATGGAAATTTTAGCCCAAGGAGGTTCTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACGCATCAGATACGTGCACATGCGAAGTATATGGGAATACCGTTACTTGGAGACGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGAACTAGAGCATCATCTAATTGTCACGGCCAACTCATGCAGCTTGTTTCTTCATTAGAGAGGCCCTGTCTTCATGCTCTGACTCTTGGGTTTGTACATCCTCACACGGGGAAGAACATACGTTTTTCATGCCCACCCCCAACAGATTTTGCTGAAATTTTGAGTCAGCTTCGTGAAATTGGCACAAAAAAGTTGCAGGTTACCAAATCTTAGCTGGGAGCCTCCAGTATCTGGCGAGTAGACTCATCGTCAAATGGCTCTAAAATGAAAGATCAAAAAGGAAATGCTCCGATTGCTCAATAACTCGTATTGAAACTGAGATTTAGCAGCATGCAACAAGAGCATAAGGTCAGCAGAAACCCCACACGATATCGGTCCTCCCCACGGTGTTTCTTCTATCCAAACTATCATGTAAGGTATCTGATATTGGTAATGATATGGTATTCGTAAGGGCACAAGGGTAGTTAGTTAAAAGTTTGCGTAGTTGGTTATAAATAGAGGGGAGCATACTTTCCCTCAGTCCCCCAATTCTCCCCTATTTGTAACCAACTACGCTAATTTCCTGGCTAACTACGCCAACGCCCTTACTAATACAATACTAATATCCGGACCTTAAATAGGTACTTTATCGTTCGTTGCTTGTGTCGAACATTTTCCTACTCTCCATGTGCAATGGAACCTCATGTTCAACTTAATGTTGATGGTAAGCCACCATTCCTATTAAACATTCGATTCCATTGCAATCTTCTTTTTCGGTTTCATATGATTTAAATAATATGTTTCGAAAGATGACAAAATTGAATGATTATTATTTTTTTAGATTTCTAATAAAAGTCA

Coding sequence (CDS)

ATGATTTCCGTTTGCTCCGCTCGAACAATTCAAGCTTCACCTTCTTCTATTCACTTCTTTTTCCCTCGAAATCGTAGGAGAATCGATTCAAATGTTCTTCGATTTTCCGCCAATTTCTCCAGTAGATGCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCCGGAACCAGACTGGAAGAGACCGTGGACATCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATATCTTCCCGCATTAACGGTATTAGTAGGGCTCGAGTTCAGTCCAGTATCAGAGCGGGATTAGTTTCAGTTAATGGCCGAGTTATCGATAAGGTTTCTCACAATGTAAAAGCTGGGGATGTGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCTCACATGGTTGTTCATCCAGCACCTGGAAATGCGACGGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCGATGGTTTCAACATCAACTGGGGAACATCAGTCGGATTCGGATGTTTCAGATGATGAGTTCAGTTCAATTGAAGTTTTTTCTAGAGTTTCTGAGGCGCCTATTCGTCCCGGTATTGTGCATAGGTTAGATAAAGGAACTAGTGGGTTGCTTGTTGTTGCCAAGGATGAACATTCCCATGCTCACTTATCTGCTCAATTCAAGCAACATTCTATTGAGAGAGTTTATATTAGTCTCACATGTGGAGTTCCACCCTCAACTGCTGGGCGTATCGAGATACCAATTTGCCGTGATCCAAACAATCGTATCCGCATGACTGCAACACATGGAACAAAAAATAATCCACATGCAAAGCACGCTGCTAGTAGATACAATGTTATGGAAATTTTAGCCCAAGGAGGTTCTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACGCATCAGATACGTGCACATGCGAAGTATATGGGAATACCGTTACTTGGAGACGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGAACTAGAGCATCATCTAATTGTCACGGCCAACTCATGCAGCTTGTTTCTTCATTAGAGAGGCCCTGTCTTCATGCTCTGACTCTTGGGTTTGTACATCCTCACACGGGGAAGAACATACGTTTTTCATGCCCACCCCCAACAGATTTTGCTGAAATTTTGAGTCAGCTTCGTGAAATTGGCACAAAAAAGTTGCAGGTTACCAAATCTTAG

Protein sequence

MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANYAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLREIGTKKLQVTKS
Homology
BLAST of MELO3C011342.jh1 vs. NCBI nr
Match: XP_008445318.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo] >XP_008445319.1 PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 861 bits (2225), Expect = 3.56e-315
Identity = 431/431 (100.00%), Postives = 431/431 (100.00%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60

Query: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG
Sbjct: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120

Query: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300
           HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA
Sbjct: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300

Query: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL
Sbjct: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420
           LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE
Sbjct: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420

Query: 421 IGTKKLQVTKS 431
           IGTKKLQVTKS
Sbjct: 421 IGTKKLQVTKS 431

BLAST of MELO3C011342.jh1 vs. NCBI nr
Match: XP_008445320.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 853 bits (2203), Expect = 5.60e-312
Identity = 429/431 (99.54%), Postives = 429/431 (99.54%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60

Query: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG
Sbjct: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120

Query: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300
           HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA
Sbjct: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300

Query: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL
Sbjct: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420
           LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE
Sbjct: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420

Query: 421 IGTKKLQVTKS 431
           IGTKK  VTKS
Sbjct: 421 IGTKK--VTKS 429

BLAST of MELO3C011342.jh1 vs. NCBI nr
Match: KGN62268.1 (hypothetical protein Csa_018737 [Cucumis sativus])

HSP 1 Score: 820 bits (2118), Expect = 5.66e-299
Identity = 412/432 (95.37%), Postives = 421/432 (97.45%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420

Query: 421 EIGTKKLQVTKS 431
           EIGT+K QVTKS
Sbjct: 421 EIGTQKWQVTKS 432

BLAST of MELO3C011342.jh1 vs. NCBI nr
Match: XP_031736623.1 (RNA pseudouridine synthase 2, chloroplastic [Cucumis sativus])

HSP 1 Score: 812 bits (2097), Expect = 1.52e-295
Identity = 407/426 (95.54%), Postives = 416/426 (97.65%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420

Query: 421 EIGTKK 425
           EIGT+K
Sbjct: 421 EIGTQK 426

BLAST of MELO3C011342.jh1 vs. NCBI nr
Match: KAA0064849.1 (RNA pseudouridine synthase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 778 bits (2010), Expect = 4.84e-283
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60

Query: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG
Sbjct: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120

Query: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300
           HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA
Sbjct: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300

Query: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL
Sbjct: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLG 391
           LRTRASSNCHGQLMQLVSSLERPCLHALTLG
Sbjct: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLG 391

BLAST of MELO3C011342.jh1 vs. ExPASy Swiss-Prot
Match: Q3ECD0 (RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g76050 PE=2 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 3.4e-143
Identity = 276/431 (64.04%), Postives = 326/431 (75.64%), Query Frame = 0

Query: 3   SVCSARTIQ--ASPS---SIHF-FFPRNRRRIDSNVLRFSANFSS--RCSDYDEYSEAQS 62
           S+ +A++++  +SPS   SI F FFP+      SN +R S+   +   CS     +E   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK-----VSNFVRASSGIPNLVACSP----TEIII 70

Query: 63  ERIANYAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVS 122
            R+ N AG R+EE VD   GK+RLDSWISSRING+SRARVQSSIR GLV+VNGRV+DKVS
Sbjct: 71  PRV-NNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVS 130

Query: 123 HNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLV 182
           HNVK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLV
Sbjct: 131 HNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLV 190

Query: 183 NAILNHCSLPMVSTSTGEHQSDSD---VSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTS 242
           N IL+HCSLP V  S  E   DSD    SDDE  +    S    A +RPGIVHRLDKGT+
Sbjct: 191 NGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTT 250

Query: 243 GLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAT 302
           GLLVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIEIPI RD +NRIRM A 
Sbjct: 251 GLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAI 310

Query: 303 HGTKNNPHAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYG 362
            G      A+HAASRY V+E  A GGS+LVEWRLETGRTHQIRAHAKYMG+PLLGDEVYG
Sbjct: 311 PGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLGDEVYG 370

Query: 363 GTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF 422
           GTKSMA+SLL+ R S +   ++++L+S ++RPCLHA+ LGF HP TG+ ++FSCPPP+D 
Sbjct: 371 GTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCPPPSDL 429

BLAST of MELO3C011342.jh1 vs. ExPASy Swiss-Prot
Match: Q5Z8P2 (RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0717400 PE=2 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 6.7e-139
Identity = 261/428 (60.98%), Postives = 312/428 (72.90%), Query Frame = 0

Query: 7   ARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQS-ERIANYAGTRL 66
           A T  ASP +I        RR      R      +RC   D  +EA +  R   + GTRL
Sbjct: 2   ATTAAASPPAIATALSALLRRQRRRSSRCVGASHARCLAADANAEAVAPSRRGGHGGTRL 61

Query: 67  EETVDINPGKVRLDSWISSRI--NGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVI 126
           EE V    G+ R+D+WIS+R+   G+SRAR+Q+SIRAGLV VNGR + KVSH VK GD++
Sbjct: 62  EEAVPAGEGRSRIDAWISARLGGGGVSRARIQASIRAGLVVVNGRPVSKVSHMVKGGDIV 121

Query: 127 NCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSL 186
           +CT+ ELQPLRAEPEDI LDIVYED+H+LVVNKPAHMVVHPAPGNA GTLVNAIL+HC +
Sbjct: 122 SCTVLELQPLRAEPEDIPLDIVYEDDHLLVVNKPAHMVVHPAPGNANGTLVNAILHHCKI 181

Query: 187 P----MVSTSTGEHQSDS-----DVSD-DEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGL 246
                +   S  +   DS     DV D D+F++ EV S V EA +RPGIVHRLDKGTSGL
Sbjct: 182 STFTCLARNSIDDECPDSSDDDIDVFDIDQFTTGEVSSEVREALVRPGIVHRLDKGTSGL 241

Query: 247 LVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHG 306
           LVVAKDEHSHA L+ QFK H+I RVYISLTCG P   +GRIE+PI RDPNNRIRM AT G
Sbjct: 242 LVVAKDEHSHAQLAEQFKLHTIRRVYISLTCGAPNPNSGRIEVPIARDPNNRIRMIATPG 301

Query: 307 TKNNPHAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGT 366
           +  + +A+HAASRY V E+ A GGS+LVEWRLETGRTHQIRAHAKY+GIPLLGDE YGGT
Sbjct: 302 S-GHRYARHAASRYKVREVFAGGGSALVEWRLETGRTHQIRAHAKYLGIPLLGDETYGGT 361

Query: 367 KSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAE 422
           KSMA+SLLR R  S  H  L  ++S ++RPCLHA  LGF HPH+GK + FSCPPP DF E
Sbjct: 362 KSMALSLLRPRTPSRYHCDLSNMISKIDRPCLHAALLGFKHPHSGKILEFSCPPPDDFTE 421

BLAST of MELO3C011342.jh1 vs. ExPASy Swiss-Prot
Match: Q45480 (Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168) OX=224308 GN=ylyB PE=3 SV=3)

HSP 1 Score: 218.4 bits (555), Expect = 1.6e-55
Identity = 135/346 (39.02%), Postives = 186/346 (53.76%), Query Frame = 0

Query: 77  RLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAE 136
           R+D +++S  N  SR +VQ  ++ G V VNG  + K ++ ++ GD +  T+ E + L   
Sbjct: 16  RIDKFLASTENDWSRTQVQQWVKDGQVVVNGSAV-KANYKIQPGDQVTVTVPEPEALDVL 75

Query: 137 PEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQSD 196
            E +DLDI YED+ VLVVNKP  MVVHPAPG+ TGTLVN ++ HC+              
Sbjct: 76  AEPMDLDIYYEDQDVLVVNKPRGMVVHPAPGHLTGTLVNGLMAHCT-------------- 135

Query: 197 SDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIE 256
                 + S I          +RPGIVHR+DK TSGLL+VAK++ +H  L  Q    ++ 
Sbjct: 136 ------DLSGI-------NGVMRPGIVHRIDKDTSGLLMVAKNDMAHESLVNQLVNKTVT 195

Query: 257 RVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQG 316
           R Y ++  G+     G I+ PI RD  +R  MT T   KN      A + ++V+E     
Sbjct: 196 RKYTAVVHGLISHDDGTIDAPIGRDKKDRQSMTVTRDGKN------AVTHFHVLERFQD- 255

Query: 317 GSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQL 376
             +LVE +LETGRTHQIR H KY+G PL GD  YG  K++           + +GQ    
Sbjct: 256 -FTLVECQLETGRTHQIRVHMKYIGFPLAGDPKYGPRKTI-----------DFNGQ---- 303

Query: 377 VSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLREIG 423
                   LHA  LGF HP TG+ + F  P P D AE++  LR+ G
Sbjct: 316 -------ALHAGVLGFDHPRTGEYVEFEAPLPEDMAELIENLRKNG 303

BLAST of MELO3C011342.jh1 vs. ExPASy Swiss-Prot
Match: P50513 (Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) OX=264203 GN=rluD PE=3 SV=2)

HSP 1 Score: 212.2 bits (539), Expect = 1.1e-53
Identity = 136/343 (39.65%), Postives = 185/343 (53.94%), Query Frame = 0

Query: 77  RLDSWISSRINGISRARVQSSIRAGLVS-VNGRVIDKVSHNVKAGDVINCTISELQPLRA 136
           RLD  ++S I  +SR R+++ I +G VS   G ++   +  +K+ D     I   +P   
Sbjct: 16  RLDRALASLITRLSRERLKNLISSGCVSNSQGALVRDPAFKIKSLDCFTVDIPLPRPAHN 75

Query: 137 EPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQS 196
           EP+DI L+IV+EDEH+LVVNKPA MVVHPA GN   TLVNA+L HC+        G+   
Sbjct: 76  EPQDIPLEIVFEDEHLLVVNKPAGMVVHPAAGNYDNTLVNALLYHCA--------GKLSG 135

Query: 197 DSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSI 256
              V+                  RPGIVHR+DK TSGLLVVAK + +HA L+AQF  HSI
Sbjct: 136 IGGVA------------------RPGIVHRIDKDTSGLLVVAKTDPAHAGLAAQFADHSI 195

Query: 257 ERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQ 316
            R Y ++  G  PS  G ++ P+ R   NR +M       ++   K A + Y ++  L  
Sbjct: 196 NRRYRAIVDG-HPSLQGHVDAPLARSSVNRQKMAIV----SDGRGKRAVTHYRMITPLK- 255

Query: 317 GGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQ 376
             +SL+E RLETGRTHQ+R H   +G  LLGD VYG +K    +LL++ A          
Sbjct: 256 -NASLIECRLETGRTHQVRVHMSSIGHSLLGDPVYGRSKKAHHALLQSLA---------- 311

Query: 377 LVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQL 419
                +R  LHA  LGF+HP +GK + F    P D   +   L
Sbjct: 316 ----FQRQALHAAHLGFIHPISGKQVDFDAEMPQDMQLLFKML 311

BLAST of MELO3C011342.jh1 vs. ExPASy Swiss-Prot
Match: O50310 (Uncharacterized RNA pseudouridine synthase Cpar_0723 OS=Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) OX=517417 GN=Cpar_0723 PE=3 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.4e-51
Identity = 130/348 (37.36%), Postives = 189/348 (54.31%), Query Frame = 0

Query: 76  VRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRA 135
           +R+D +++ ++  ++R +VQ++I  G V VNG+ + K ++ VK+ D I+ T         
Sbjct: 12  MRIDRYLTQQVENMTRNKVQAAIEEGRVLVNGKPV-KSNYRVKSCDHIHLTFLRPPAPEL 71

Query: 136 EPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQS 195
            PEDI + I+YED+ ++V++K A MVVHPA GN TGTL NAIL+H               
Sbjct: 72  APEDIPITILYEDDDLMVIDKEAGMVVHPAFGNWTGTLANAILHHLG------------K 131

Query: 196 DSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSI 255
           D+D  D             +  +RPGIVHRLDK TSGL+++AK+  +   L+ QF    +
Sbjct: 132 DADELD-------------KTEMRPGIVHRLDKDTSGLIIIAKNPTALHKLARQFANRQV 191

Query: 256 ERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQ 315
           E+VY ++  GVP + +G I+  I R   NR  M   +        KHA + Y V+E L  
Sbjct: 192 EKVYKAIVWGVPKAESGTIKTNIGRSHKNRKVM--ANFPYEGAEGKHAITDYQVVEDL-- 251

Query: 316 GGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQ 375
           G  SL++  L TGRTHQIR H +++G  +LGDE YGG         RT   S        
Sbjct: 252 GYFSLMDVTLHTGRTHQIRVHLQHLGHSILGDETYGGATP------RTLPFSKSEPFTRN 311

Query: 376 LVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLREIGT 424
           L+  + R  LHA TL F  P TG+ + FS P P D   +L ++R + T
Sbjct: 312 LLELMSRQALHAQTLRFRQPTTGEPLSFSAPLPEDMELVLEKIRTVMT 323

BLAST of MELO3C011342.jh1 vs. ExPASy TrEMBL
Match: A0A1S3BCE4 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 861 bits (2225), Expect = 1.73e-315
Identity = 431/431 (100.00%), Postives = 431/431 (100.00%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60

Query: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG
Sbjct: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120

Query: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300
           HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA
Sbjct: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300

Query: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL
Sbjct: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420
           LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE
Sbjct: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420

Query: 421 IGTKKLQVTKS 431
           IGTKKLQVTKS
Sbjct: 421 IGTKKLQVTKS 431

BLAST of MELO3C011342.jh1 vs. ExPASy TrEMBL
Match: A0A1S3BD56 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 853 bits (2203), Expect = 2.71e-312
Identity = 429/431 (99.54%), Postives = 429/431 (99.54%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60

Query: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG
Sbjct: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120

Query: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300
           HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA
Sbjct: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300

Query: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL
Sbjct: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420
           LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE
Sbjct: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420

Query: 421 IGTKKLQVTKS 431
           IGTKK  VTKS
Sbjct: 421 IGTKK--VTKS 429

BLAST of MELO3C011342.jh1 vs. ExPASy TrEMBL
Match: A0A0A0LKD7 (Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1)

HSP 1 Score: 820 bits (2118), Expect = 2.74e-299
Identity = 412/432 (95.37%), Postives = 421/432 (97.45%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420

Query: 421 EIGTKKLQVTKS 431
           EIGT+K QVTKS
Sbjct: 421 EIGTQKWQVTKS 432

BLAST of MELO3C011342.jh1 vs. ExPASy TrEMBL
Match: A0A5A7VHA3 (Pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001610 PE=3 SV=1)

HSP 1 Score: 778 bits (2010), Expect = 2.35e-283
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60

Query: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG
Sbjct: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120

Query: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300
           HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA
Sbjct: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300

Query: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL
Sbjct: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLG 391
           LRTRASSNCHGQLMQLVSSLERPCLHALTLG
Sbjct: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLG 391

BLAST of MELO3C011342.jh1 vs. ExPASy TrEMBL
Match: A0A6J1CMS3 (Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1)

HSP 1 Score: 728 bits (1878), Expect = 8.66e-263
Identity = 366/427 (85.71%), Postives = 390/427 (91.33%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60
           MISVCS R IQA+PS I  FFPRNR RI SNV R + N S+RCSDYDE  EA SERI NY
Sbjct: 1   MISVCSVRAIQAAPS-IQLFFPRNRGRITSNVHRIATNHSTRCSDYDECGEAHSERITNY 60

Query: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AG RLEE VD+N GK+RLDSWIS RINGISRARVQSSIRAGLV+VNGRVIDKVSHN+KAG
Sbjct: 61  AGMRLEEIVDVNSGKIRLDSWISCRINGISRARVQSSIRAGLVTVNGRVIDKVSHNIKAG 120

Query: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           D+INCTISELQPLRA+PEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DMINCTISELQPLRAKPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFS-RVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           CSLPMV+TS GE  SD D+SDDEFSS E+ S R SEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 CSLPMVATSNGE-LSDMDISDDEFSSNEILSSRASEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRM+A   T  NPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPICRDPNNRIRMSAVLRTNKNPH 300

Query: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRY V+EILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMA+S
Sbjct: 301 AKYAASRYKVIEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMALS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420
           LLRTR SS+CH QLMQLVS+LERPCLHALTLGF+HP+TG+NIRFSCPPPTDFA+I SQL 
Sbjct: 361 LLRTRTSSSCHSQLMQLVSALERPCLHALTLGFIHPYTGENIRFSCPPPTDFADIYSQLC 420

Query: 421 EIGTKKL 426
           EIGTKK+
Sbjct: 421 EIGTKKV 425

BLAST of MELO3C011342.jh1 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 509.6 bits (1311), Expect = 2.4e-144
Identity = 276/431 (64.04%), Postives = 326/431 (75.64%), Query Frame = 0

Query: 3   SVCSARTIQ--ASPS---SIHF-FFPRNRRRIDSNVLRFSANFSS--RCSDYDEYSEAQS 62
           S+ +A++++  +SPS   SI F FFP+      SN +R S+   +   CS     +E   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK-----VSNFVRASSGIPNLVACSP----TEIII 70

Query: 63  ERIANYAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVS 122
            R+ N AG R+EE VD   GK+RLDSWISSRING+SRARVQSSIR GLV+VNGRV+DKVS
Sbjct: 71  PRV-NNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVS 130

Query: 123 HNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLV 182
           HNVK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLV
Sbjct: 131 HNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLV 190

Query: 183 NAILNHCSLPMVSTSTGEHQSDSD---VSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTS 242
           N IL+HCSLP V  S  E   DSD    SDDE  +    S    A +RPGIVHRLDKGT+
Sbjct: 191 NGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTT 250

Query: 243 GLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAT 302
           GLLVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIEIPI RD +NRIRM A 
Sbjct: 251 GLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAI 310

Query: 303 HGTKNNPHAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYG 362
            G      A+HAASRY V+E  A GGS+LVEWRLETGRTHQIRAHAKYMG+PLLGDEVYG
Sbjct: 311 PGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLGDEVYG 370

Query: 363 GTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF 422
           GTKSMA+SLL+ R S +   ++++L+S ++RPCLHA+ LGF HP TG+ ++FSCPPP+D 
Sbjct: 371 GTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCPPPSDL 429

BLAST of MELO3C011342.jh1 vs. TAIR 10
Match: AT1G76050.1 (Pseudouridine synthase family protein )

HSP 1 Score: 341.3 bits (874), Expect = 1.2e-93
Identity = 199/315 (63.17%), Postives = 230/315 (73.02%), Query Frame = 0

Query: 3   SVCSARTIQ--ASPS---SIHF-FFPRNRRRIDSNVLRFSANFSS--RCSDYDEYSEAQS 62
           S+ +A++++  +SPS   SI F FFP+      SN +R S+   +   CS     +E   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK-----VSNFVRASSGIPNLVACSP----TEIII 70

Query: 63  ERIANYAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVS 122
            R+ N AG R+EE VD   GK+RLDSWISSRING+SRARVQSSIR GLV+VNGRV+DKVS
Sbjct: 71  PRV-NNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVS 130

Query: 123 HNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLV 182
           HNVK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLV
Sbjct: 131 HNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLV 190

Query: 183 NAILNHCSLPMVSTSTGEHQSDSD---VSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTS 242
           N IL+HCSLP V  S  E   DSD    SDDE  +    S    A +RPGIVHRLDKGT+
Sbjct: 191 NGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTT 250

Query: 243 GLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAT 302
           GLLVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIEIPI RD +NRIRM A 
Sbjct: 251 GLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAI 310

Query: 303 HGTKNNPHAKHAASR 307
            G      A+HAASR
Sbjct: 311 PGGVRGGRARHAASR 313

BLAST of MELO3C011342.jh1 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 74.7 bits (182), Expect = 2.0e-13
Identity = 69/272 (25.37%), Postives = 118/272 (43.38%), Query Frame = 0

Query: 98  IRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKP 157
           I+ G + ++G V+   +  +++G  +   +    P +       L+++YED+ ++ +NKP
Sbjct: 56  IQNGQIQIDGEVVKDPNTLLRSGSKL---VYSRLPWKEPDTPYSLEVLYEDDDLIALNKP 115

Query: 158 AHMVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAP 217
           + + V P                  L  +    G++ S             + SR S  P
Sbjct: 116 SGLQVLPG---------GLFQQRTVLTQLQWCFGKNDS------------YIGSRESPHP 175

Query: 218 IRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIERVYI 277
           +    VHRL +GTSG+L+ AK + +   L+A F +                   + ++Y 
Sbjct: 176 VP---VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYR 235

Query: 278 SLTCGVPPSTAGRIEIPI--CRDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQGGS 337
           +L  G+       I+ PI   R P       A      +P  K A S+  V+E   +   
Sbjct: 236 ALADGIVEEDEVVIKQPIGVVRYPG-----VAQGLYVASPEGKPAFSKVFVLERDREKNC 295

Query: 338 SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVY 351
           SLV+  +++GR HQIR H  YMG PL+GD +Y
Sbjct: 296 SLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY 295

BLAST of MELO3C011342.jh1 vs. TAIR 10
Match: AT1G56345.1 (Pseudouridine synthase family protein )

HSP 1 Score: 70.1 bits (170), Expect = 5.0e-12
Identity = 74/301 (24.58%), Postives = 108/301 (35.88%), Query Frame = 0

Query: 143 DIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQSDSDVSDD 202
           DI+YEDE+++ VNKP             G    A+L      ++ +S+  H         
Sbjct: 76  DILYEDEYLMAVNKP------------KGVYCEAVLRSAPQIVLDSSSEYH--------- 135

Query: 203 EFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISL 262
                              + +RLD+ TSG++++ K     A L   F +H I + YI+L
Sbjct: 136 -------------------LANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRKSYIAL 195

Query: 263 TCGVPPS------------------------TAGRIEIP---ICRDPNNRIRMTATHGTK 322
             G  P+                          GR+ +P     RD      + + +  K
Sbjct: 196 CIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRV-LPGGSFVRDMETTFEVVSVNSVK 255

Query: 323 NNPHAKHAASRYNVME---ILAQGGSS-----LVEWRLETGRTHQIRAHAKYMGIPLLGD 382
           N        +   V E    L+ GG       +V     +GRTHQIR H +Y+GIP+ GD
Sbjct: 256 NESCELEDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQYLGIPIRGD 315

Query: 383 EVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPP 409
             Y                   HG       + E   LHA  L   HP TG +I    P 
Sbjct: 316 VKY-------------------HGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPL 316

BLAST of MELO3C011342.jh1 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 66.6 bits (161), Expect = 5.5e-11
Identity = 66/266 (24.81%), Postives = 113/266 (42.48%), Query Frame = 0

Query: 98  IRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKP 157
           I+ G + ++G V+   +  +++G  +   +    P +       L+++YED+ ++ +NKP
Sbjct: 56  IQNGQIQIDGEVVKDPNTLLRSGSKL---VYSRLPWKEPDTPYSLEVLYEDDDLIALNKP 115

Query: 158 AHMVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAP 217
           + + V P                  L  +    G++ S             + SR S  P
Sbjct: 116 SGLQVLPG---------GLFQQRTVLTQLQWCFGKNDS------------YIGSRESPHP 175

Query: 218 IRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIERVYI 277
           +    VHRL +GTSG+L+ AK + +   L+A F +                   + ++Y 
Sbjct: 176 VP---VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYR 235

Query: 278 SLTCGVPPSTAGRIEIPI--CRDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQGGS 337
           +L  G+       I+ PI   R P       A      +P  K A S+  V+E   +   
Sbjct: 236 ALADGIVEEDEVVIKQPIGVVRYPG-----VAQGLYVASPEGKPAFSKVFVLERDREKNC 289

Query: 338 SLVEWRLETGRTHQIRAHAKYMGIPL 345
           SLV+  +++GR HQIR H  YMG PL
Sbjct: 296 SLVKVEIQSGRPHQIRIHLAYMGHPL 289

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008445318.13.56e-315100.00PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo]... [more]
XP_008445320.15.60e-31299.54PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis melo][more]
KGN62268.15.66e-29995.37hypothetical protein Csa_018737 [Cucumis sativus][more]
XP_031736623.11.52e-29595.54RNA pseudouridine synthase 2, chloroplastic [Cucumis sativus][more]
KAA0064849.14.84e-283100.00RNA pseudouridine synthase 2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q3ECD03.4e-14364.04RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q5Z8P26.7e-13960.98RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=3... [more]
Q454801.6e-5539.02Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168... [more]
P505131.1e-5339.65Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mob... [more]
O503101.4e-5137.36Uncharacterized RNA pseudouridine synthase Cpar_0723 OS=Chlorobaculum parvum (st... [more]
Match NameE-valueIdentityDescription
A0A1S3BCE41.73e-315100.00Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1[more]
A0A1S3BD562.71e-31299.54Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1[more]
A0A0A0LKD72.74e-29995.37Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1[more]
A0A5A7VHA32.35e-283100.00Pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A6J1CMS38.66e-26385.71Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G76050.22.4e-14464.04Pseudouridine synthase family protein [more]
AT1G76050.11.2e-9363.17Pseudouridine synthase family protein [more]
AT3G52260.22.0e-1325.37Pseudouridine synthase family protein [more]
AT1G56345.15.0e-1224.58Pseudouridine synthase family protein [more]
AT3G52260.15.5e-1124.81Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002942RNA-binding S4 domainSMARTSM00363s4_6coord: 76..138
e-value: 9.8E-7
score: 38.4
IPR002942RNA-binding S4 domainPFAMPF01479S4coord: 76..123
e-value: 1.6E-9
score: 37.3
IPR002942RNA-binding S4 domainCDDcd00165S4coord: 76..155
e-value: 5.04586E-14
score: 64.193
IPR006225Pseudouridine synthase, RluC/RluDTIGRFAMTIGR00005TIGR00005coord: 76..332
e-value: 4.3E-58
score: 194.9
IPR036986RNA-binding S4 domain superfamilyGENE3D3.10.290.10coord: 61..131
e-value: 2.3E-16
score: 61.0
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 150..332
e-value: 1.8E-26
score: 93.1
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 151..332
e-value: 1.95782E-48
score: 159.04
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 133..332
e-value: 2.2E-47
score: 163.4
NoneNo IPR availablePANTHERPTHR21600:SF57RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE Dcoord: 75..332
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 75..332
NoneNo IPR availablePROSITEPS50889S4coord: 76..149
score: 12.485687
NoneNo IPR availableSUPERFAMILY55174Alpha-L RNA-binding motifcoord: 75..163
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 223..237
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 136..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C011342.jh1.t1MELO3C011342.jh1.t1mRNA
MELO3C011342.jh1.t2MELO3C011342.jh1.t2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding