Homology
BLAST of MELO3C010940 vs. NCBI nr
Match:
XP_008444711.1 (PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo])
HSP 1 Score: 1311.2 bits (3392), Expect = 0.0e+00
Identity = 641/641 (100.00%), Postives = 641/641 (100.00%), Query Frame = 0
Query: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL
Sbjct: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
Query: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180
AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180
Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
Query: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
BLAST of MELO3C010940 vs. NCBI nr
Match:
KAA0065238.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo var. makuwa])
HSP 1 Score: 1307.4 bits (3382), Expect = 0.0e+00
Identity = 638/641 (99.53%), Postives = 640/641 (99.84%), Query Frame = 0
Query: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL
Sbjct: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
Query: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180
AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMK+LGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180
Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCA SLVGEPIMYPEINTLVDELHQRRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCAFSLVGEPIMYPEINTLVDELHQRRIS 420
Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
Query: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
DSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
BLAST of MELO3C010940 vs. NCBI nr
Match:
XP_004152698.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis sativus] >KGN62597.1 hypothetical protein Csa_021988 [Cucumis sativus])
HSP 1 Score: 1283.1 bits (3319), Expect = 0.0e+00
Identity = 626/641 (97.66%), Postives = 636/641 (99.22%), Query Frame = 0
Query: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
MS SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTL SRKPKLFFISQTGTSKAL
Sbjct: 1 MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
Query: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180
AEDFRVGSLLLSQCKFSVFGVGSRVYGET+NAVARDFSKR+K+LGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
DIDKCFDDWSRKLLKV+K DAEGNGVELCSGIAGDSDAVSVEE+ EEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEED-EEEDDDLGEEDIVDL 240
Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
EDIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVN 360
Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDV 540
Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPF 600
Query: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
DSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 640
BLAST of MELO3C010940 vs. NCBI nr
Match:
XP_038884961.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida] >XP_038884963.1 S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa hispida])
HSP 1 Score: 1256.9 bits (3251), Expect = 0.0e+00
Identity = 612/641 (95.48%), Postives = 632/641 (98.60%), Query Frame = 0
Query: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
MSFSSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPTLSSR+PKLFFISQTGTSKAL
Sbjct: 1 MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60
Query: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
AHRLLNVLNSN LVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61 AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180
AEDFRVGSLLLS+CKF+VFGVGSR YGET+NAVAR+FSKRMK+LGAKEIL +G+GDVDGG
Sbjct: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180
Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
DID+CFDDWSR+LLKV+KVDA GNGV+L SGI GDSDA SVEE+ EEED DLGEEDIVDL
Sbjct: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEED-EEEDADLGEEDIVDL 240
Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
SAIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480
Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
Query: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
DSKDY+APTPSWAVYGSDEGGFDP+QSRYRKERHH+PKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKPTS 640
BLAST of MELO3C010940 vs. NCBI nr
Match:
XP_022997485.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita maxima])
HSP 1 Score: 1228.4 bits (3177), Expect = 0.0e+00
Identity = 599/641 (93.45%), Postives = 619/641 (96.57%), Query Frame = 0
Query: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
MSFSSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT SR+PKLFFISQTGTSKAL
Sbjct: 1 MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
Query: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
AHRLLNVLNSN L FD+VDPKD+EPEDLFKE LVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61 AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120
Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180
AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVARDFSKRMK+LGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
DIDKCFDDWSR+LLKV+KVDA G G +L SGI D DA SVEE ++ED D GEEDIVDL
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEE--DDEDADPGEEDIVDL 240
Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
TFLVTNAQFPDKIK LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQ
Sbjct: 421 TFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480
Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
QRTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
Query: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
DSKDY+APTPSWAVYG+DEGGFDP QSRYRKERHH+PKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639
BLAST of MELO3C010940 vs. ExPASy Swiss-Prot
Match:
Q8RXN5 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis thaliana OX=3702 GN=TYW1 PE=2 SV=1)
HSP 1 Score: 965.3 bits (2494), Expect = 3.4e-280
Identity = 470/644 (72.98%), Postives = 539/644 (83.70%), Query Frame = 0
Query: 4 SSRSAKLTILALLSASTLYCFYKSRRLRKLK-LSLNP--TLSSRKPKLFFISQTGTSKAL 63
SS +L +ALLSA+T YC +K RRL+ LK LSLNP TL + + K+FFISQTGT+KAL
Sbjct: 5 SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64
Query: 64 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 123
A RL + SN + FD+VDP YEPEDL KETLVLF+ASTW+ G PP + +FL NWL ES
Sbjct: 65 AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124
Query: 124 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 183
AEDFRVGSLLLS CKF+VFGVGSR YGE+YNAVA++ S RM LG E++P+GEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184
Query: 184 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEY-----EEEDDDLGEE 243
++D+ F DW +++V+K G + +G++ AV + EY EE++D+ +
Sbjct: 185 ELDRAFQDWCDGVIRVLK----GGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADG 244
Query: 244 DIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICR 303
IVDLEDIAGK PS+++ V T K++GKK MVTPVIRASL KQGYKIIGSHSGVKICR
Sbjct: 245 GIVDLEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICR 304
Query: 304 WTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDP 363
WTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P
Sbjct: 305 WTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEP 364
Query: 364 LDIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH 423
IV A+DLH MIKQMKGVPGVT EKL+EGL+PRHCALSLVGEPIMYPEIN LVDELH
Sbjct: 365 SVIVKGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELH 424
Query: 424 QRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKA 483
RRISTFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKA
Sbjct: 425 GRRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKA 484
Query: 484 LNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVP 543
L EKQQRTVYRLTLVKGWNTE++DAY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVP
Sbjct: 485 LQEKQQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVP 544
Query: 544 WHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVA 603
WH+DVK+FSEAL+ KS GEYEVACEH HSCCVLL +T+KF+VDG+WFTWIDYEKFHDLVA
Sbjct: 545 WHTDVKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVA 604
Query: 604 SGKPFDSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKP 640
SG+PF S DY+A TPSWAVYG+ EGGFDP Q RY+KER+H PKP
Sbjct: 605 SGEPFTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKP 642
BLAST of MELO3C010940 vs. ExPASy Swiss-Prot
Match:
Q8H8N3 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativa subsp. japonica OX=39947 GN=TYW1 PE=2 SV=1)
HSP 1 Score: 879.0 bits (2270), Expect = 3.2e-254
Identity = 434/642 (67.60%), Postives = 510/642 (79.44%), Query Frame = 0
Query: 4 SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLS--SRKPKLFFISQTGTSKALA 63
+S ++ L +L LLS+S+++ YKS RLR+ P + P L + S TGTSKALA
Sbjct: 12 ASTTSHLALLLLLSSSSVFFLYKSLRLRRNNPPSPPPGQGPAPTPTLLYASATGTSKALA 71
Query: 64 HRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSESA 123
L L G+ D ++P+DL L+L V T + G PPP A FLA WL ESA
Sbjct: 72 AGLSRRLAEAGVTAHPADAAAFDPDDLPSLPLLLLVLPTHDGGAPPPAAAFLARWLEESA 131
Query: 124 EDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGGD 183
DFR G+ LLS +F+VFGVGSR YGET+NA AR FS+ +++LGA E++ +GEGDVDGGD
Sbjct: 132 ADFRAGAALLSGLRFAVFGVGSRAYGETFNAAARSFSRWLRALGAAEVVAVGEGDVDGGD 191
Query: 184 IDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDI---- 243
++ F++W ++++VVK G E+ G G+SD E E EE DDD EE++
Sbjct: 192 LEVVFEEWCGRVVRVVK------GEEIGEGHNGESDGFD-ELEEEESDDDDDEEEVDGGE 251
Query: 244 VDLEDIAGKGP-SRKSTNVVE---TNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKI 303
VD+EDIAGK P +R+ VE +NG NG + MVTP+IR SLEKQGYKIIGSHSGVKI
Sbjct: 252 VDMEDIAGKAPAARRRNGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKI 311
Query: 304 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMD 363
CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+W+MD
Sbjct: 312 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMD 371
Query: 364 DPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDE 423
DPLDIVN+AID HTKM+KQMKGVPGV E+L EGLSPRHCALSLVGEPIMYPEIN L+DE
Sbjct: 372 DPLDIVNAAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDE 431
Query: 424 LHQRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSL 483
LH+R ISTFLVTNAQFPDKIK LKP+TQLYVSVDAATKESLKA+DRPLF DFWERF+DSL
Sbjct: 432 LHRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSL 491
Query: 484 KALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMEN 543
K+L++K QRTVYRLTLVKGWN E+ID Y+ L S+G+PDF+EIKGVTYCGSS TSKLTMEN
Sbjct: 492 KSLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMEN 551
Query: 544 VPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDL 603
VPWHSDVK FSEALA KS G YEVACEH HSCCVLLAK DKF+++G+W TWIDY++FH+L
Sbjct: 552 VPWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHEL 611
Query: 604 VASGKPFDSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHH 636
V SGKPF S+DY+A TPSWAVYG++EGGFDPDQSRY+KER H
Sbjct: 612 VTSGKPFRSQDYMALTPSWAVYGAEEGGFDPDQSRYKKERRH 646
BLAST of MELO3C010940 vs. ExPASy Swiss-Prot
Match:
Q2KHP8 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopus laevis OX=8355 GN=tyw1 PE=2 SV=1)
HSP 1 Score: 629.8 bits (1623), Expect = 3.4e-179
Identity = 335/677 (49.48%), Postives = 437/677 (64.55%), Query Frame = 0
Query: 30 LRKLKLSLNPTLSSRKP--------KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPK 89
L K K + +L+S K K+F+ SQTGTSK AH L + GL +L++ K
Sbjct: 59 LLKTKALKSDSLTSNKEQKVYVAGVKIFYGSQTGTSKGFAHLLAEEVTLLGLPVELINMK 118
Query: 90 DYEPED-LFKET----LVLFVASTWEDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKF 149
+Y+P+D L +ET + +F+ +T+ DG PP A++ WL E++ DFR G L ++
Sbjct: 119 EYDPDDNLVEETTSKNICVFLVATYTDGKPPESAEWFCKWLEEASNDFRFGKTYLKGMRY 178
Query: 150 SVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVD-----GGDIDKCFDDWSR 209
+VFG+G+ VY YN V ++ K + L A ++ EGD + G I+ F+ W R
Sbjct: 179 AVFGLGNSVYSTHYNTVGKNIDKWLWMLSANRVMTRAEGDCNVVKSKHGSIESDFEAWKR 238
Query: 210 KLLKVVKVDAEGNGVELCSG--------IAGDSDAVSVEEEYEEEDDDLGEED------- 269
K L +K G + CSG S SVEEE EEE+ ED
Sbjct: 239 KFLNRLKALLSGE-KKPCSGKCKKGKCKSKKKSSIESVEEEEEEEEKHSEHEDTEDDTFE 298
Query: 270 ----------------IVDLEDIAGKGPS--RKSTNVVETNGKLNGKKV----------- 329
++D+ED+ GK S +KS ++N +L GK +
Sbjct: 299 TSSDSEPEEHGEPGSGLIDVEDL-GKAMSNMKKSKREHDSNTEL-GKSLQGDEAKEKEEP 358
Query: 330 --MVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATP 389
M+TP ++ +L KQGY++IGSHSGVK+CRWTKS LRGRGGCYKH+FYGIESHRCME TP
Sbjct: 359 REMITPALQEALTKQGYRLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTP 418
Query: 390 SLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLE 449
SLACANKCVFCWRHHTNPVG W+W+MD P I+ AI H MIKQ KGVPGV E+ E
Sbjct: 419 SLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILEEAILNHQNMIKQFKGVPGVKPERFE 478
Query: 450 EGLSPRHCALSLVGEPIMYPEINTLVDELHQRRISTFLVTNAQFPDKIKLLKPVTQLYVS 509
EGL+ +HCALSLVGEPIMYPEINT + LH + IS+FLVTNAQFP++I+ L+PVTQLYVS
Sbjct: 479 EGLAVKHCALSLVGEPIMYPEINTFLRLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVS 538
Query: 510 VDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLF 569
VDA+TK+SLK IDRPLF DFW+RFIDSLKAL EKQQRTVYRLTLVK WN +++ AY++L
Sbjct: 539 VDASTKDSLKKIDRPLFKDFWQRFIDSLKALAEKQQRTVYRLTLVKAWNVDELKAYADLV 598
Query: 570 SIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSC 629
++G PDF+E+KGVTYCG S+ S LTM NVPWH +V F E L +YEVACEH HS
Sbjct: 599 ALGLPDFIEVKGVTYCGESSASNLTMANVPWHEEVIRFVEEL-VDLLPDYEVACEHEHSN 658
Query: 630 CVLLAKTDKFRVDGQWFTWIDYEKFHDLVAS------GKPFDSKDYIAPTPSWAVYGSDE 637
C+L+A KF+++G+W TWIDYE+F +L+ + + F + DY+A TP WA++GS E
Sbjct: 659 CMLIAH-KKFKINGEWCTWIDYERFQELIQAFGESKGSRTFTALDYVAKTPEWALFGSRE 718
BLAST of MELO3C010940 vs. ExPASy Swiss-Prot
Match:
Q08C92 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio rerio OX=7955 GN=tyw1 PE=2 SV=1)
HSP 1 Score: 620.5 bits (1599), Expect = 2.1e-176
Identity = 316/649 (48.69%), Postives = 427/649 (65.79%), Query Frame = 0
Query: 47 KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKE-----TLVLFVASTW 106
K+F+ SQTGT+K A L + + G+ +++D KD++PED E + +F+ +T+
Sbjct: 80 KVFYGSQTGTAKGFAKELAEDVIAQGIQCEVIDMKDFDPEDRLAEECTSKIICVFLVATY 139
Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRM 166
DG P A++ WL E++ DFR G L +++VFG+G+ VY +N V++ K +
Sbjct: 140 TDGQPTESAEWFCKWLEEASTDFRYGKTYLKGMRYAVFGLGNSVYVGHFNTVSKSIDKWL 199
Query: 167 KSLGAKEILPIGEGDVD-----GGDIDKCFDDWSRKLLKVVKVDAEGNGVELCSG----- 226
L A I+ GEGD + G + F W K L ++ A+G + CSG
Sbjct: 200 WMLSAARIMTRGEGDCNVVKSRHGSVQADFQVWKGKFLNRLQALAKGE-KKACSGNCKKA 259
Query: 227 ----------IAGDSDAVSVEEEYEEE------DDDLGEED------IVDLEDIAG---- 286
A D+ +++ + EEE D++ ED ++D+ED+
Sbjct: 260 SCKNKKKHKEEAEDNHSLAEKNNSEEELMESSSDEESSSEDEKSHGSVIDMEDLGNVMNH 319
Query: 287 --KGPSR-----KSTNVVETNGKLNG-----KKVMVTPVIRASLEKQGYKIIGSHSGVKI 346
K R + + V+ NG+ ++ M+TP +R SL KQGYK+IGSHSGVK+
Sbjct: 320 MKKAKQRMEEDEEDSQRVKQNGERKSECEEERREMITPALRDSLTKQGYKLIGSHSGVKL 379
Query: 347 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMD 406
RWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG W+W+MD
Sbjct: 380 RRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMD 439
Query: 407 DPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDE 466
I+ A++ H MI+Q +GVPGV E+ EEGL+ +HCALSLVGEPIMYPEIN+ +
Sbjct: 440 PAEKIIQEAMENHRNMIRQFRGVPGVRPERFEEGLTVKHCALSLVGEPIMYPEINSFLKL 499
Query: 467 LHQRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSL 526
LHQ+ IS+FLVTNAQFP++I+ L PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+DSL
Sbjct: 500 LHQQNISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSL 559
Query: 527 KALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMEN 586
+AL EKQQRTVYRLTLVK WN +++ AY++L ++G+PDF+E+KGVTYCG S+ S LTM N
Sbjct: 560 RALGEKQQRTVYRLTLVKAWNVDELKAYADLIALGQPDFIEVKGVTYCGESSASSLTMAN 619
Query: 587 VPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDL 637
VPWH +V F + L A +YE+ACEH HS C+LLA KF+VDG+W+TWIDYE+F +L
Sbjct: 620 VPWHEEVIYFVQQL-ANLLPDYEIACEHEHSNCLLLA-NHKFKVDGEWWTWIDYERFQEL 679
BLAST of MELO3C010940 vs. ExPASy Swiss-Prot
Match:
Q9NV66 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Homo sapiens OX=9606 GN=TYW1 PE=2 SV=2)
HSP 1 Score: 610.1 bits (1572), Expect = 2.8e-173
Identity = 309/650 (47.54%), Postives = 415/650 (63.85%), Query Frame = 0
Query: 47 KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPED-----LFKETLVLFVASTW 106
K+F+ SQTGT+K A L + S L +++ K+Y+P+D + + + +F+ +T+
Sbjct: 80 KIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATY 139
Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRM 166
DG P A++ WL E++ DFR G L +++VFG+G+ Y +N V ++ K +
Sbjct: 140 TDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWL 199
Query: 167 KSLGAKEILPIGEGDVD-----GGDIDKCFDDWSRKLLKVVKVDAEGNGVELCSGI---- 226
LGA ++ GEGD D G I+ F W K + ++ +G + C G
Sbjct: 200 WMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKG 259
Query: 227 ---------------AGDSDAVSVEEEYEEE------DDDLGEED------IVDLEDIAG 286
+ + D + + EEE +++ G ED IVD+ED+
Sbjct: 260 KCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGK 319
Query: 287 -----KGPSRKSTNVVETNGKLNG--------KKVMVTPVIRASLEKQGYKIIGSHSGVK 346
K R+ E +G ++ M+TP +R +L KQGY++IGSHSGVK
Sbjct: 320 IMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVK 379
Query: 347 ICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEM 406
+CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG W+W+M
Sbjct: 380 LCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKM 439
Query: 407 DDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVD 466
D P I+ AI+ H MIKQ KGVPGV E+ EEG++ +HCALSLVGEPIMYPEIN +
Sbjct: 440 DQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLK 499
Query: 467 ELHQRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 526
LHQ +IS+FLVTNAQFP +I+ L+PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+DS
Sbjct: 500 LLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDS 559
Query: 527 LKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTME 586
LKAL KQQRTVYRLTLVK WN +++ AY+ L S+G PDF+E+KGVTYCG S+ S LTM
Sbjct: 560 LKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMA 619
Query: 587 NVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHD 637
+VPWH +V F L EYE+ACEH HS C+L+A KF++ G+W+TWIDY +F +
Sbjct: 620 HVPWHEEVVQFVHELVDLIP-EYEIACEHEHSNCLLIAHR-KFKIGGEWWTWIDYNRFQE 679
BLAST of MELO3C010940 vs. ExPASy TrEMBL
Match:
A0A1S3BAY2 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo OX=3656 GN=LOC103487965 PE=3 SV=1)
HSP 1 Score: 1311.2 bits (3392), Expect = 0.0e+00
Identity = 641/641 (100.00%), Postives = 641/641 (100.00%), Query Frame = 0
Query: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL
Sbjct: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
Query: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180
AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180
Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
Query: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
BLAST of MELO3C010940 vs. ExPASy TrEMBL
Match:
A0A5A7VC83 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005790 PE=3 SV=1)
HSP 1 Score: 1307.4 bits (3382), Expect = 0.0e+00
Identity = 638/641 (99.53%), Postives = 640/641 (99.84%), Query Frame = 0
Query: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL
Sbjct: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
Query: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180
AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMK+LGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180
Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCA SLVGEPIMYPEINTLVDELHQRRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCAFSLVGEPIMYPEINTLVDELHQRRIS 420
Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
Query: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
DSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
BLAST of MELO3C010940 vs. ExPASy TrEMBL
Match:
A0A0A0LPA4 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis sativus OX=3659 GN=Csa_2G361620 PE=3 SV=1)
HSP 1 Score: 1283.1 bits (3319), Expect = 0.0e+00
Identity = 626/641 (97.66%), Postives = 636/641 (99.22%), Query Frame = 0
Query: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
MS SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTL SRKPKLFFISQTGTSKAL
Sbjct: 1 MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
Query: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180
AEDFRVGSLLLSQCKFSVFGVGSRVYGET+NAVARDFSKR+K+LGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
DIDKCFDDWSRKLLKV+K DAEGNGVELCSGIAGDSDAVSVEE+ EEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEED-EEEDDDLGEEDIVDL 240
Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
EDIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVN 360
Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDV 540
Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPF 600
Query: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
DSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 640
BLAST of MELO3C010940 vs. ExPASy TrEMBL
Match:
A0A6J1K9S7 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita maxima OX=3661 GN=LOC111492394 PE=3 SV=1)
HSP 1 Score: 1228.4 bits (3177), Expect = 0.0e+00
Identity = 599/641 (93.45%), Postives = 619/641 (96.57%), Query Frame = 0
Query: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
MSFSSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT SR+PKLFFISQTGTSKAL
Sbjct: 1 MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
Query: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
AHRLLNVLNSN L FD+VDPKD+EPEDLFKE LVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61 AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120
Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180
AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVARDFSKRMK+LGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
DIDKCFDDWSR+LLKV+KVDA G G +L SGI D DA SVEE ++ED D GEEDIVDL
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEE--DDEDADPGEEDIVDL 240
Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
TFLVTNAQFPDKIK LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQ
Sbjct: 421 TFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480
Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
QRTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
Query: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
DSKDY+APTPSWAVYG+DEGGFDP QSRYRKERHH+PKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639
BLAST of MELO3C010940 vs. ExPASy TrEMBL
Match:
A0A6J1HDZ5 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita moschata OX=3662 GN=LOC111462691 PE=3 SV=1)
HSP 1 Score: 1219.1 bits (3153), Expect = 0.0e+00
Identity = 594/641 (92.67%), Postives = 617/641 (96.26%), Query Frame = 0
Query: 1 MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60
MS SSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT SR+PKLFFISQTGTSKAL
Sbjct: 1 MSLSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
Query: 61 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
AHRLLNVLNSN L FD+VDPKD+EPEDLFKETLVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61 AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180
AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVARDFSKRMK+LGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
Query: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240
DIDKCFDDWSR+LLKV+KVDA G G +L SGI D DA SVEE ++ED D GEEDIVDL
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEE--DDEDADPGEEDIVDL 240
Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420
SAIDLH KMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
TFLVTNAQFPDKIK LKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFIDSLKALN+KQ
Sbjct: 421 TFLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIDSLKALNDKQ 480
Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
QRTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFR+DGQWFTWIDYEKFH LVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRIDGQWFTWIDYEKFHALVASGKPF 600
Query: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 642
DSKDY+APTPSWAVYG+DEGGFDP QSRYRKERHH+PKPTS
Sbjct: 601 DSKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639
BLAST of MELO3C010940 vs. TAIR 10
Match:
AT1G75200.1 (flavodoxin family protein / radical SAM domain-containing protein )
HSP 1 Score: 965.3 bits (2494), Expect = 2.4e-281
Identity = 470/644 (72.98%), Postives = 539/644 (83.70%), Query Frame = 0
Query: 4 SSRSAKLTILALLSASTLYCFYKSRRLRKLK-LSLNP--TLSSRKPKLFFISQTGTSKAL 63
SS +L +ALLSA+T YC +K RRL+ LK LSLNP TL + + K+FFISQTGT+KAL
Sbjct: 5 SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64
Query: 64 AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 123
A RL + SN + FD+VDP YEPEDL KETLVLF+ASTW+ G PP + +FL NWL ES
Sbjct: 65 AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124
Query: 124 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 183
AEDFRVGSLLLS CKF+VFGVGSR YGE+YNAVA++ S RM LG E++P+GEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184
Query: 184 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEY-----EEEDDDLGEE 243
++D+ F DW +++V+K G + +G++ AV + EY EE++D+ +
Sbjct: 185 ELDRAFQDWCDGVIRVLK----GGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADG 244
Query: 244 DIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICR 303
IVDLEDIAGK PS+++ V T K++GKK MVTPVIRASL KQGYKIIGSHSGVKICR
Sbjct: 245 GIVDLEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICR 304
Query: 304 WTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDP 363
WTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P
Sbjct: 305 WTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEP 364
Query: 364 LDIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH 423
IV A+DLH MIKQMKGVPGVT EKL+EGL+PRHCALSLVGEPIMYPEIN LVDELH
Sbjct: 365 SVIVKGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELH 424
Query: 424 QRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKA 483
RRISTFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKA
Sbjct: 425 GRRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKA 484
Query: 484 LNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVP 543
L EKQQRTVYRLTLVKGWNTE++DAY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVP
Sbjct: 485 LQEKQQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVP 544
Query: 544 WHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVA 603
WH+DVK+FSEAL+ KS GEYEVACEH HSCCVLL +T+KF+VDG+WFTWIDYEKFHDLVA
Sbjct: 545 WHTDVKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVA 604
Query: 604 SGKPFDSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKP 640
SG+PF S DY+A TPSWAVYG+ EGGFDP Q RY+KER+H PKP
Sbjct: 605 SGEPFTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKP 642
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_008444711.1 | 0.0e+00 | 100.00 | PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cu... | [more] |
KAA0065238.1 | 0.0e+00 | 99.53 | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo ... | [more] |
XP_004152698.1 | 0.0e+00 | 97.66 | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis sativ... | [more] |
XP_038884961.1 | 0.0e+00 | 95.48 | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Benincasa his... | [more] |
XP_022997485.1 | 0.0e+00 | 93.45 | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita max... | [more] |
Match Name | E-value | Identity | Description | |
Q8RXN5 | 3.4e-280 | 72.98 | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis... | [more] |
Q8H8N3 | 3.2e-254 | 67.60 | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativ... | [more] |
Q2KHP8 | 3.4e-179 | 49.48 | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopu... | [more] |
Q08C92 | 2.1e-176 | 48.69 | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio ... | [more] |
Q9NV66 | 2.8e-173 | 47.54 | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Homo s... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3BAY2 | 0.0e+00 | 100.00 | tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo OX=3656 GN=LO... | [more] |
A0A5A7VC83 | 0.0e+00 | 99.53 | tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo var. makuwa O... | [more] |
A0A0A0LPA4 | 0.0e+00 | 97.66 | tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis sativus OX=3659 GN... | [more] |
A0A6J1K9S7 | 0.0e+00 | 93.45 | tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita maxima OX=3661 G... | [more] |
A0A6J1HDZ5 | 0.0e+00 | 92.67 | tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita moschata OX=3662... | [more] |
Match Name | E-value | Identity | Description | |
AT1G75200.1 | 2.4e-281 | 72.98 | flavodoxin family protein / radical SAM domain-containing protein | [more] |