MELO3C009858.jh1 (gene) Melon (Harukei-3) v1.41

Overview


Name	MELO3C009858.jh1
Type	gene
Organism	Cucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Description	Calcium-binding EF hand family protein
Location	chr04: 28538606 .. 28539358 (+)
RNA-Seq Expression	MELO3C009858.jh1
Synteny	MELO3C009858.jh1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonstart_codonCDSpolypeptidestop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

AACTCAGTCCCCAAACAAAATTCTATATCCATGTTCTTTAGATTAGAGTACCATTTAATTACAATGCAAACGCAGGTAACCAACGCTGTCAAGAGCGTGCAGCTCGACCCGTCACCGCTCGTTTTTGTCGATTGGATATCAAATCTACCAATTTTATTCAACTTTGCAGGCAGAATACATGAGCTGATTAGATCTCTACCTTATTGGTCAATTCTTCAATCACAGTACTCCAAAGAGAAAGCTTTATCACAAAGTAATTGCTCTTCACAATCTGATGATAAAATCATCATCACTACTCAGCAAAATTGCTCAATGAGCAGAGAGGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGGAGGGAAGAAAATGATGATGATGATGATGATGATGATGATGAAGAAATTGGGGAAAGTGATGAAATTATAAGAGGTATGTTTGAAGAGAATGAACCAAGTTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAAAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATAGCCAGATTTGATCTCAATAATGATGGTAAAATTGATTTCCATGAATTTGTTAAGTTTATGGAAGTTGCTTTGTGTTAGAATATTTTGCTTCTACTATTATAATTATTTGTAATAACTTTTTTCATTTTTATTACAGACATTGTTCTTGAATGA

mRNA sequence

AACTCAGTCCCCAAACAAAATTCTATATCCATGTTCTTTAGATTAGAGTACCATTTAATTACAATGCAAACGCAGGTAACCAACGCTGTCAAGAGCGTGCAGCTCGACCCGTCACCGCTCGTTTTTGTCGATTGGATATCAAATCTACCAATTTTATTCAACTTTGCAGGCAGAATACATGAGCTGATTAGATCTCTACCTTATTGGTCAATTCTTCAATCACAGTACTCCAAAGAGAAAGCTTTATCACAAAGTAATTGCTCTTCACAATCTGATGATAAAATCATCATCACTACTCAGCAAAATTGCTCAATGAGCAGAGAGGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGGAGGGAAGAAAATGATGATGATGATGATGATGATGATGATGAAGAAATTGGGGAAAGTGATGAAATTATAAGAGGTATGTTTGAAGAGAATGAACCAAGTTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAAAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATAGCCAGATTTGATCTCAATAATGATGGTAAAATTGATTTCCATGAATTTGTTAAGTTTATGGAAGTTGCTTTGTGTTAGAATATTTTGCTTCTACTATTATAATTATTTGTAATAACTTTTTTCATTTTTATTACAGACATTGTTCTTGAATGA

Coding sequence (CDS)

ATGTTCTTTAGATTAGAGTACCATTTAATTACAATGCAAACGCAGGTAACCAACGCTGTCAAGAGCGTGCAGCTCGACCCGTCACCGCTCGTTTTTGTCGATTGGATATCAAATCTACCAATTTTATTCAACTTTGCAGGCAGAATACATGAGCTGATTAGATCTCTACCTTATTGGTCAATTCTTCAATCACAGTACTCCAAAGAGAAAGCTTTATCACAAAGTAATTGCTCTTCACAATCTGATGATAAAATCATCATCACTACTCAGCAAAATTGCTCAATGAGCAGAGAGGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGGAGGGAAGAAAATGATGATGATGATGATGATGATGATGATGAAGAAATTGGGGAAAGTGATGAAATTATAAGAGGTATGTTTGAAGAGAATGAACCAAGTTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAAAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATAGCCAGATTTGATCTCAATAATGATGGTAAAATTGATTTCCATGAATTTGTTAAGTTTATGGAAGTTGCTTTGTGTTAG

Protein sequence

MFFRLEYHLITMQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDDDDEEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALC

Homology

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BLAST of MELO3C009858.jh1 vs. NCBI nr
Match: XP_008443616.1 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 389 bits (1000), Expect = 4.58e-136
Identity = 200/204 (98.04%), Postives = 200/204 (98.04%), Query Frame = 0


Query: 12  MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 71
           MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 72  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDDDDEEIGESD 131
           LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDD   EEIG SD
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDD---EEIGASD 120

Query: 132 EIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIA 191
           EIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIA
Sbjct: 121 EIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIA 180

Query: 192 RFDLNNDGKIDFHEFVKFMEVALC 215
           RFDLNNDGKIDFHEFVKFMEVALC
Sbjct: 181 RFDLNNDGKIDFHEFVKFMEVALC 201

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BLAST of MELO3C009858.jh1 vs. NCBI nr
Match: KGN59556.1 (hypothetical protein Csa_001548 [Cucumis sativus])

HSP 1 Score: 327 bits (837), Expect = 4.33e-111
Identity = 180/215 (83.72%), Postives = 193/215 (89.77%), Query Frame = 0


Query: 1   MFFRLEYHLITMQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWS 60
           MFFRLEY+LITM+T++T+ +KSVQLDPSP VFVD IS LPILFNFA RI EL+RSLP W 
Sbjct: 1   MFFRLEYYLITMETRLTD-IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLP-WP 60

Query: 61  ILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCS-MSREEVRFVMEKLELFWREENDDDD 120
           ILQ  +SKEKALSQ NCSS+S D+IIITTQQNCS MSREEVRFVMEKLELFWREE D   
Sbjct: 61  ILQP-HSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGG 120

Query: 121 DDDDDEEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNK 180
            D   + IGESDE+IRGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNK
Sbjct: 121 GD---KGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNK 180

Query: 181 GIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 214
           GIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL
Sbjct: 181 GIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 209

[Close]

BLAST of MELO3C009858.jh1 vs. NCBI nr
Match: XP_004147503.1 (probable calcium-binding protein CML45 [Cucumis sativus])

HSP 1 Score: 308 bits (788), Expect = 8.56e-104
Identity = 170/204 (83.33%), Postives = 182/204 (89.22%), Query Frame = 0


Query: 12  MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 71
           M+T++T+ +KSVQLDPSP VFVD IS LPILFNFA RI EL+RSLP W ILQ  +SKEKA
Sbjct: 1   METRLTD-IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLP-WPILQP-HSKEKA 60

Query: 72  LSQSNCSSQSDDKIIITTQQNCS-MSREEVRFVMEKLELFWREENDDDDDDDDDEEIGES 131
           LSQ NCSS+S D+IIITTQQNCS MSREEVRFVMEKLELFWREE D    D   + IGES
Sbjct: 61  LSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGD---KGIGES 120

Query: 132 DEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMI 191
           DE+IRGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNKGIFIHDCKTMI
Sbjct: 121 DEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMI 180

Query: 192 ARFDLNNDGKIDFHEFVKFMEVAL 214
           ARFDLNNDGKIDFHEFVKFMEVAL
Sbjct: 181 ARFDLNNDGKIDFHEFVKFMEVAL 198

[Close]

BLAST of MELO3C009858.jh1 vs. NCBI nr
Match: XP_038904514.1 (probable calcium-binding protein CML45 [Benincasa hispida])

HSP 1 Score: 233 bits (595), Expect = 1.59e-74
Identity = 139/195 (71.28%), Postives = 151/195 (77.44%), Query Frame = 0


Query: 22  SVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQS 81
           +VQL P+PLVFVD  S LPILF FA RIH+LI S+P  S  QS  SKEKA  QSNCSSQS
Sbjct: 9   NVQLIPTPLVFVDRTSILPILFTFADRIHDLI-SVPL-SFFQSP-SKEKASPQSNCSSQS 68

Query: 82  DDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDDDDEEIGE--SDEIIRGMFE 141
           DD++I T Q+NC MSREEVRFVM+KLELF  +        D DEEIGE    E +  MFE
Sbjct: 69  DDQVI-TQQKNCLMSREEVRFVMDKLELFCSKN-------DGDEEIGECFGGEELTAMFE 128

Query: 142 ENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDG 201
           ENEPSLEELKQTFNVFDRN+DGFID  EL IVL LL+SN GIFIHDCK MIARFD NNDG
Sbjct: 129 ENEPSLEELKQTFNVFDRNRDGFIDAEELHIVLGLLQSNNGIFIHDCKRMIARFDHNNDG 188

Query: 202 KIDFHEFVKFMEVAL 214
           KIDF EFVKFMEVAL
Sbjct: 189 KIDFTEFVKFMEVAL 192

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BLAST of MELO3C009858.jh1 vs. NCBI nr
Match: XP_023528818.1 (probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 178 bits (452), Expect = 8.50e-53
Identity = 123/217 (56.68%), Postives = 139/217 (64.06%), Query Frame = 0


Query: 1   MFFRLEYHLITMQTQVT---NAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLP 60
           MFF+LEY L     Q T     + S      PLVF+D  S       FA  IH+LI  + 
Sbjct: 1   MFFKLEYLLTMHNPQPTINLQLIPSTTTTTPPLVFIDRAS------IFADTIHDLISLV- 60

Query: 61  YWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDD 120
            +S  QS   KEKA  QS  S QS+ K      ++  MSREEV+ VMEKLEL+  EE DD
Sbjct: 61  -FSFFQSP-PKEKAAPQSRAS-QSEGK------EDWLMSREEVKSVMEKLELYCNEEEDD 120

Query: 121 DDDDDDDEEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLES 180
           + D     E    DE+ RGMFEENEPSLEELKQTFNVFDRN+DGFID  EL  VL LL S
Sbjct: 121 EID-----ECFGRDEV-RGMFEENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLAS 180

Query: 181 NKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 214
           N  IFIHDCK+MIARFD NND KIDF+EFVKFMEVAL
Sbjct: 181 NNTIFIHDCKSMIARFDHNNDQKIDFNEFVKFMEVAL 195

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BLAST of MELO3C009858.jh1 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 5.4e-20
Identity = 67/182 (36.81%), Postives = 96/182 (52.75%), Query Frame = 0


Query: 32  FVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQ 91
           F  W+S+  I   F  R   L++        ++   +++ L++       DD        
Sbjct: 23  FCRWVSSTRI---FLSRFVPLLQHHQRVFDKKNNKDQQETLTKQEDDDDDDDDDDDDDDD 82

Query: 92  NCSMSREEVRFVMEKLELFWREENDDDDDDDDDEEIGESDEIIRGMFEENEPSLEELKQT 151
           +  +SREE   VM  L LF+         +DD  +   S + +  +FEE E SLEE+KQ 
Sbjct: 83  DIDISREEAEMVMRSLGLFY---------NDDQLQEQYSAKEVSSLFEEKEASLEEVKQA 142

Query: 152 FNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 211
           F+VFD NKDGFID  EL  VL++L   +G ++ +C  MI   D N DGKIDF+EFVKFME
Sbjct: 143 FDVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFME 192

Query: 212 VA 214
            +
Sbjct: 203 TS 192

[Close]

BLAST of MELO3C009858.jh1 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-16
Identity = 70/218 (32.11%), Postives = 105/218 (48.17%), Query Frame = 0


Query: 7   YHLITMQTQVTNAVKSVQLDPSPLV---------FVDWISNLPILFNFAGRIHELIRSLP 66
           Y  ITM++ ++   +S  L   PL          F  W+S   + F+             
Sbjct: 7   YSFITMKSSLSKCKQSSSLS-FPLFGLINFFLIGFFRWVSFAQLFFS------------R 66

Query: 67  YWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDD 126
           +W ++Q Q    +  S+      S          +  + RE+V  VM+ L L   +EN+ 
Sbjct: 67  FWPLVQHQQCVSEKKSKDLEFQTSIKHEEYRDDDDDGLCREDVGMVMKSLGLSTDQENEG 126

Query: 127 DDDDDDDEEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLES 186
                  +E+         +FEE EPSLEE+KQ F+VFD N+DGFID  +L  VL++L  
Sbjct: 127 LQKQYSSKEVS-------NLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGL 186

Query: 187 NKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALC 216
            +G  + +C+ MI  FD + DG+IDF+ FVKFME   C
Sbjct: 187 KQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFMENNFC 204

[Close]

BLAST of MELO3C009858.jh1 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 36/94 (38.30%), Postives = 58/94 (61.70%), Query Frame = 0


Query: 118 DDDDDDDEEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLES 177
           D + D   +I E  E+ + + +E +   E++K+ FNVFD+N DGFI   EL  VLS L  
Sbjct: 50  DVNGDGCVDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGL 109

Query: 178 NKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 212
            +G  + DCK MI + D++ DG++++ EF + M+
Sbjct: 110 KQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

[Close]

BLAST of MELO3C009858.jh1 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.2e-08
Identity = 47/129 (36.43%), Postives = 75/129 (58.14%), Query Frame = 0


Query: 84  KIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDDDDEEIGESDEIIRGMFEENEP 143
           K+++  + +   S EEV+ ++            DD +   +  I E +E    + E+NE 
Sbjct: 67  KVVVPNRADIKTSVEEVKAII------------DDSEALYECLIEEGEEY---LLEKNEM 126

Query: 144 SLEEL-KQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKID 203
             +E+ K+ F +FD N+DGFIDE+EL  VLSLL  ++   + +C+ M+  +D N DGKID
Sbjct: 127 MGKEIVKEAFRLFDENQDGFIDENELKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKID 179

Query: 204 FHEFVKFME 212
           F+EFVK +E
Sbjct: 187 FYEFVKLIE 179

[Close]

BLAST of MELO3C009858.jh1 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.6e-08
Identity = 35/93 (37.63%), Postives = 56/93 (60.22%), Query Frame = 0


Query: 118 DDDDDDDEEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLES 177
           D + D   +I E   + + M EE E   E++++ F VFD+N DGFI + EL  VL+ +  
Sbjct: 50  DVNGDGAMDIDEFGSLYQEMVEEKEEE-EDMREAFRVFDQNGDGFITDEELRSVLASMGL 109

Query: 178 NKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFM 211
            +G  + DCK MI++ D++ DG ++F EF + M
Sbjct: 110 KQGRTLEDCKKMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of MELO3C009858.jh1 vs. ExPASy TrEMBL
Match: A0A1S3B8I1 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 PE=4 SV=1)

HSP 1 Score: 389 bits (1000), Expect = 2.22e-136
Identity = 200/204 (98.04%), Postives = 200/204 (98.04%), Query Frame = 0


Query: 12  MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 71
           MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 72  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDDDDEEIGESD 131
           LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDD   EEIG SD
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDD---EEIGASD 120

Query: 132 EIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIA 191
           EIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIA
Sbjct: 121 EIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIA 180

Query: 192 RFDLNNDGKIDFHEFVKFMEVALC 215
           RFDLNNDGKIDFHEFVKFMEVALC
Sbjct: 181 RFDLNNDGKIDFHEFVKFMEVALC 201

[Close]

BLAST of MELO3C009858.jh1 vs. ExPASy TrEMBL
Match: A0A0A0LHQ5 (Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G825010 PE=4 SV=1)

HSP 1 Score: 327 bits (837), Expect = 2.10e-111
Identity = 180/215 (83.72%), Postives = 193/215 (89.77%), Query Frame = 0


Query: 1   MFFRLEYHLITMQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWS 60
           MFFRLEY+LITM+T++T+ +KSVQLDPSP VFVD IS LPILFNFA RI EL+RSLP W 
Sbjct: 1   MFFRLEYYLITMETRLTD-IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLP-WP 60

Query: 61  ILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCS-MSREEVRFVMEKLELFWREENDDDD 120
           ILQ  +SKEKALSQ NCSS+S D+IIITTQQNCS MSREEVRFVMEKLELFWREE D   
Sbjct: 61  ILQP-HSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGG 120

Query: 121 DDDDDEEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNK 180
            D   + IGESDE+IRGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNK
Sbjct: 121 GD---KGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNK 180

Query: 181 GIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 214
           GIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL
Sbjct: 181 GIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 209

[Close]

BLAST of MELO3C009858.jh1 vs. ExPASy TrEMBL
Match: A0A6J1J5Q6 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111481990 PE=4 SV=1)

HSP 1 Score: 176 bits (446), Expect = 2.51e-52
Identity = 115/190 (60.53%), Postives = 129/190 (67.89%), Query Frame = 0


Query: 27  PSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKII 86
           P PLVF+D  S       FA  IH+L+ SL + S  QS   KEKA  QS  S QS+ K  
Sbjct: 21  PPPLVFIDRAS------IFADTIHDLV-SLAF-SFFQSP-PKEKAAPQSRAS-QSEGK-- 80

Query: 87  ITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDDDDEEIGE--SDEIIRGMFEENEPS 146
               ++  MSREEV+ VMEKLEL+  EE DD        EI E    E +RGMFEENEPS
Sbjct: 81  ----EDWLMSREEVKSVMEKLELYCNEEGDD--------EIDECFGREEVRGMFEENEPS 140

Query: 147 LEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFH 206
           LEELKQTFNVFDRN+DGFID HEL  VL LL SN  IFIHDCK+MIARFD NND KIDF+
Sbjct: 141 LEELKQTFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNNDEKIDFN 186

Query: 207 EFVKFMEVAL 214
           EFVKFMEV+L
Sbjct: 201 EFVKFMEVSL 186

[Close]

BLAST of MELO3C009858.jh1 vs. ExPASy TrEMBL
Match: A0A6J1F854 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111441729 PE=4 SV=1)

HSP 1 Score: 173 bits (439), Expect = 2.79e-51
Identity = 114/186 (61.29%), Postives = 129/186 (69.35%), Query Frame = 0


Query: 29  PLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKIIIT 88
           PLVF+D  S       FAG IH+L+ SL + S  QS   K+KA  QS  S QS+ K    
Sbjct: 22  PLVFIDRAS------IFAGTIHDLV-SLAF-SFFQSP-PKDKAAPQSRAS-QSESK---- 81

Query: 89  TQQNCSMSREEVRFVMEKLELFWREENDDDDDDDDDEEIGESDEIIRGMFEENEPSLEEL 148
             ++  MSREEV+ VMEKLEL+  EE DD+ D     E    DE+ RGMFEENEPSLEEL
Sbjct: 82  --EDWLMSREEVKSVMEKLELYCNEEEDDEID-----ECFGRDEV-RGMFEENEPSLEEL 141

Query: 149 KQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVK 208
           KQTFNVFDRN+DGFID  EL  VL LL SN  IFIHDCK+MIARFD NND KIDF+EFVK
Sbjct: 142 KQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVK 185

Query: 209 FMEVAL 214
           FMEVAL
Sbjct: 202 FMEVAL 185

[Close]

BLAST of MELO3C009858.jh1 vs. ExPASy TrEMBL
Match: A0A6J1EIK8 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111434428 PE=4 SV=1)

HSP 1 Score: 163 bits (412), Expect = 3.27e-47
Identity = 107/188 (56.91%), Postives = 125/188 (66.49%), Query Frame = 0


Query: 30  LVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKIIITT 89
           L+ VD IS   ILF+F  RIH+LI S P WS+  S   KEKA  Q N S +S+     T 
Sbjct: 16  LLLVDRISISSILFSFVDRIHDLI-SQP-WSLFHSP-PKEKASPQGN-SLESE----ATQ 75

Query: 90  QQNCSMSREEVRFVMEKLELFWREENDDDDDDDDDEEIGES---DEIIRGMFEENEPSLE 149
            +N S+S+EEV+FVMEKLE F             DEEI ES    E I  +F E+EPSLE
Sbjct: 76  HKNSSISKEEVKFVMEKLEFFCS-----------DEEIEESWLGGEEIGAIFYESEPSLE 135

Query: 150 ELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEF 209
           ELKQTFNVFD+N+DG ID  EL  VL LL  N+G+FIHDC  MIARFD N DGKIDF+EF
Sbjct: 136 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 184

Query: 210 VKFMEVAL 214
           VKFME+AL
Sbjct: 196 VKFMEIAL 184

[Close]

BLAST of MELO3C009858.jh1 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 99.4 bits (246), Expect = 3.8e-21
Identity = 67/182 (36.81%), Postives = 96/182 (52.75%), Query Frame = 0


Query: 32  FVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQ 91
           F  W+S+  I   F  R   L++        ++   +++ L++       DD        
Sbjct: 23  FCRWVSSTRI---FLSRFVPLLQHHQRVFDKKNNKDQQETLTKQEDDDDDDDDDDDDDDD 82

Query: 92  NCSMSREEVRFVMEKLELFWREENDDDDDDDDDEEIGESDEIIRGMFEENEPSLEELKQT 151
           +  +SREE   VM  L LF+         +DD  +   S + +  +FEE E SLEE+KQ 
Sbjct: 83  DIDISREEAEMVMRSLGLFY---------NDDQLQEQYSAKEVSSLFEEKEASLEEVKQA 142

Query: 152 FNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 211
           F+VFD NKDGFID  EL  VL++L   +G ++ +C  MI   D N DGKIDF+EFVKFME
Sbjct: 143 FDVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFME 192

Query: 212 VA 214
            +
Sbjct: 203 TS 192

[Close]

BLAST of MELO3C009858.jh1 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 88.2 bits (217), Expect = 8.8e-18
Identity = 70/218 (32.11%), Postives = 105/218 (48.17%), Query Frame = 0


Query: 7   YHLITMQTQVTNAVKSVQLDPSPLV---------FVDWISNLPILFNFAGRIHELIRSLP 66
           Y  ITM++ ++   +S  L   PL          F  W+S   + F+             
Sbjct: 7   YSFITMKSSLSKCKQSSSLS-FPLFGLINFFLIGFFRWVSFAQLFFS------------R 66

Query: 67  YWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDD 126
           +W ++Q Q    +  S+      S          +  + RE+V  VM+ L L   +EN+ 
Sbjct: 67  FWPLVQHQQCVSEKKSKDLEFQTSIKHEEYRDDDDDGLCREDVGMVMKSLGLSTDQENEG 126

Query: 127 DDDDDDDEEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLES 186
                  +E+         +FEE EPSLEE+KQ F+VFD N+DGFID  +L  VL++L  
Sbjct: 127 LQKQYSSKEVS-------NLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGL 186

Query: 187 NKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALC 216
            +G  + +C+ MI  FD + DG+IDF+ FVKFME   C
Sbjct: 187 KQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFMENNFC 204

[Close]

BLAST of MELO3C009858.jh1 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 65.1 bits (157), Expect = 8.0e-11
Identity = 36/94 (38.30%), Postives = 58/94 (61.70%), Query Frame = 0


Query: 118 DDDDDDDEEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLES 177
           D + D   +I E  E+ + + +E +   E++K+ FNVFD+N DGFI   EL  VLS L  
Sbjct: 50  DVNGDGCVDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGL 109

Query: 178 NKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 212
            +G  + DCK MI + D++ DG++++ EF + M+
Sbjct: 110 KQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

[Close]

BLAST of MELO3C009858.jh1 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 61.6 bits (148), Expect = 8.8e-10
Identity = 47/129 (36.43%), Postives = 75/129 (58.14%), Query Frame = 0


Query: 84  KIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDDDDEEIGESDEIIRGMFEENEP 143
           K+++  + +   S EEV+ ++            DD +   +  I E +E    + E+NE 
Sbjct: 67  KVVVPNRADIKTSVEEVKAII------------DDSEALYECLIEEGEEY---LLEKNEM 126

Query: 144 SLEEL-KQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKID 203
             +E+ K+ F +FD N+DGFIDE+EL  VLSLL  ++   + +C+ M+  +D N DGKID
Sbjct: 127 MGKEIVKEAFRLFDENQDGFIDENELKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKID 179

Query: 204 FHEFVKFME 212
           F+EFVK +E
Sbjct: 187 FYEFVKLIE 179

[Close]

BLAST of MELO3C009858.jh1 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 60.1 bits (144), Expect = 2.6e-09
Identity = 35/93 (37.63%), Postives = 56/93 (60.22%), Query Frame = 0


Query: 118 DDDDDDDEEIGESDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLES 177
           D + D   +I E   + + M EE E   E++++ F VFD+N DGFI + EL  VL+ +  
Sbjct: 50  DVNGDGAMDIDEFGSLYQEMVEEKEEE-EDMREAFRVFDQNGDGFITDEELRSVLASMGL 109

Query: 178 NKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFM 211
            +G  + DCK MI++ D++ DG ++F EF + M
Sbjct: 110 KQGRTLEDCKKMISKVDVDGDGMVNFKEFKQMM 141

The following BLAST results are available for this feature:

BLAST of MELO3C009858.jh1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008443616.1	4.58e-136	98.04	PREDICTED: probable calcium-binding protein CML30 [Cucumis melo]	[more]
KGN59556.1	4.33e-111	83.72	hypothetical protein Csa_001548 [Cucumis sativus]	[more]
XP_004147503.1	8.56e-104	83.33	probable calcium-binding protein CML45 [Cucumis sativus]	[more]
XP_038904514.1	1.59e-74	71.28	probable calcium-binding protein CML45 [Benincasa hispida]	[more]
XP_023528818.1	8.50e-53	56.68	probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MELO3C009858.jh1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG5	5.4e-20	36.81	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q93Z27	1.2e-16	32.11	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9LNE7	1.1e-09	38.30	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SN89	1.2e-08	36.43	Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ...	[more]
Q9SU00	3.6e-08	37.63	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]

BLAST of MELO3C009858.jh1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B8I1	2.22e-136	98.04	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 P...	[more]
A0A0A0LHQ5	2.10e-111	83.72	Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G82501...	[more]
A0A6J1J5Q6	2.51e-52	60.53	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114819...	[more]
A0A6J1F854	2.79e-51	61.29	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1EIK8	3.27e-47	56.91	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]

BLAST of MELO3C009858.jh1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29000.1	3.8e-21	36.81	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	8.8e-18	32.11	Calcium-binding EF-hand family protein	[more]
AT1G05990.1	8.0e-11	38.30	EF hand calcium-binding protein family	[more]
AT3G47480.1	8.8e-10	36.43	Calcium-binding EF-hand family protein	[more]
AT4G12860.1	2.6e-09	37.63	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 185..213 e-value: 8.7E-4 score: 28.6 coord: 147..175 e-value: 2.2E-5 score: 33.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 146..211 e-value: 1.6E-9 score: 38.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 181..215 score: 11.054376
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 143..178 score: 13.760229
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 147..210 e-value: 1.80271E-15 score: 66.4173
None	No IPR available	GENE3D	1.10.238.10		coord: 79..214 e-value: 1.6E-18 score: 69.2
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 16..211
None	No IPR available	PANTHER	PTHR10891:SF745	CALCIUM-BINDING PROTEIN CML46-RELATED	coord: 16..211
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 156..168
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 194..206
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 139..210

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C009858.jh1.t1	MELO3C009858.jh1.t1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding