MELO3C009584 (gene) Melon (DHL92) v4

BLAST of MELO3C009584 vs. NCBI nr
Match: XP_008442988.1 (PREDICTED: probable glutamate carboxypeptidase 2 isoform X1 [Cucumis melo] >KAA0043770.1 putative glutamate carboxypeptidase 2 isoform X1 [Cucumis melo var. makuwa] >TYK25363.1 putative glutamate carboxypeptidase 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1403.3 bits (3631), Expect = 0.0e+00
Identity = 701/701 (100.00%), Postives = 701/701 (100.00%), Query Frame = 0

Query: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60
           MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL
Sbjct: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60

Query: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
           LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK
Sbjct: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120

Query: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180
           STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG
Sbjct: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180

Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240
           VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300
           GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300

Query: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
           AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG
Sbjct: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360

Query: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
           TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN
Sbjct: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420

Query: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
           VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS
Sbjct: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480

Query: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
           DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL
Sbjct: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540

Query: 541 SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE 600
           SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE
Sbjct: 541 SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE 600

Query: 601 QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA 660
           QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA
Sbjct: 601 QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA 660

Query: 661 DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 702
           DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS
Sbjct: 661 DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 701

BLAST of MELO3C009584 vs. NCBI nr
Match: XP_004136724.3 (LOW QUALITY PROTEIN: probable glutamate carboxypeptidase AMP1 [Cucumis sativus])

HSP 1 Score: 1339.7 bits (3466), Expect = 0.0e+00
Identity = 671/701 (95.72%), Postives = 684/701 (97.57%), Query Frame = 0

Query: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60
           MVQ PLKQLATICTSRPAPLPTFFFVIIICVLGFY FHFS+SSSFSVTSSPRNSVRFQQL
Sbjct: 1   MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFSVTSSPRNSVRFQQL 60

Query: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
           LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK
Sbjct: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120

Query: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180
           STSLSVL SNG+VVNIPLSENVEGVVQPYHAYSPSGT YGPAVFVNYGRDEDYRELAKMG
Sbjct: 121 STSLSVLLSNGTVVNIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMG 180

Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240
           VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLY E D FRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300
           GWAAVDGAERLNLNDSEVLKRFPKIPSMPLS+ESAEIIL+SLDTASVPPEWR K+ANL S
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWR-KKANLGS 300

Query: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
           AAVGPGGP+FINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG
Sbjct: 301 AAVGPGGPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360

Query: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
           TAALLDIARRF+LLR+LGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN
Sbjct: 361 TAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420

Query: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
           VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS
Sbjct: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480

Query: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
           DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL
Sbjct: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540

Query: 541 SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE 600
           SDDLILPFSY+SYANQLQAYKDTLNHLLDGSVSL +LS+SIEELK AAQEIENEAKRLRE
Sbjct: 541 SDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLRE 600

Query: 601 QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA 660
           QE SSDVALFQKRALNDRLMLAERGFLDVDGLRG PWFKHLVYGP S+YESALVYFPGIA
Sbjct: 601 QEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIA 660

Query: 661 DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 702
           DAVSESKE+NK  LEELIQHEIWRVARAI RAAAALKGELS
Sbjct: 661 DAVSESKEINKRELEELIQHEIWRVARAIRRAAAALKGELS 700

BLAST of MELO3C009584 vs. NCBI nr
Match: XP_038905595.1 (probable glutamate carboxypeptidase AMP1 [Benincasa hispida])

HSP 1 Score: 1285.4 bits (3325), Expect = 0.0e+00
Identity = 639/701 (91.16%), Postives = 665/701 (94.86%), Query Frame = 0

Query: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60
           M QPPLKQLATICTS+PAPLPTF FV+IICVLGF+ FHFSS SSFS TS+P NSVRFQQL
Sbjct: 1   MAQPPLKQLATICTSKPAPLPTFLFVVIICVLGFFTFHFSSLSSFSATSTPSNSVRFQQL 60

Query: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
           LLSS SNYTVASYLRSLTLHPHLAGTEPS ETV+YVESHFR+LGLETHSIQYDALLSYPK
Sbjct: 61  LLSSASNYTVASYLRSLTLHPHLAGTEPSLETVQYVESHFRELGLETHSIQYDALLSYPK 120

Query: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180
           S SLS    NGSVVNIPLSE VEGVVQPYHAYSPSGT YG AVFVNYGRDEDYR LAK+G
Sbjct: 121 SASLSARLPNGSVVNIPLSEYVEGVVQPYHAYSPSGTAYGTAVFVNYGRDEDYRALAKIG 180

Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240
           V V GCIAVARKGEFPRGVVVAKAEANGAKGVLLYTE D FRQGFERGTVMRGIGDPLSP
Sbjct: 181 VAVDGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEGDGFRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300
           GWAA+DGAERL+LNDSEVLKRFPKIPSMPLS+E+AEIIL+SLD+ASVPPEWRD +  L S
Sbjct: 241 GWAAIDGAERLSLNDSEVLKRFPKIPSMPLSAEAAEIILSSLDSASVPPEWRDMKGKLGS 300

Query: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
           AAVGPGGP FINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG
Sbjct: 301 AAVGPGGPTFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360

Query: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
           TAALLDIARRFALLR+LGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN
Sbjct: 361 TAALLDIARRFALLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420

Query: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
           VDCAVQGPGFF+GATPQLDDLLH+VTAQVQDPDVKGATV+DTWTA+NGIGNIERLGA+NS
Sbjct: 421 VDCAVQGPGFFSGATPQLDDLLHEVTAQVQDPDVKGATVYDTWTAKNGIGNIERLGALNS 480

Query: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
           DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLF RHVTVGSIWGLLALRL
Sbjct: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFQRHVTVGSIWGLLALRL 540

Query: 541 SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE 600
           SDDLILPFSYVSYANQLQAY+DTLN +LDGSVSL TLSTSI+ELKSAAQEIENEAKRLRE
Sbjct: 541 SDDLILPFSYVSYANQLQAYQDTLNDVLDGSVSLHTLSTSIQELKSAAQEIENEAKRLRE 600

Query: 601 QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA 660
           QE  SDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFP IA
Sbjct: 601 QEIFSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPSIA 660

Query: 661 DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 702
           DAVSESK+MNK  +EE+IQHEIWRVARAI RAA ALKGELS
Sbjct: 661 DAVSESKKMNKREMEEVIQHEIWRVARAIRRAALALKGELS 701

BLAST of MELO3C009584 vs. NCBI nr
Match: KAE8651122.1 (hypothetical protein Csa_002557 [Cucumis sativus])

HSP 1 Score: 1252.3 bits (3239), Expect = 0.0e+00
Identity = 654/828 (78.99%), Postives = 673/828 (81.28%), Query Frame = 0

Query: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60
           MVQ PLKQLATICTSRPAPLPTFFFVIIICVLGFY FHFS+SSSFSVTSSPRNSVRFQQL
Sbjct: 1   MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFSVTSSPRNSVRFQQL 60

Query: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
           LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK
Sbjct: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120

Query: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180
           STSLSVL SNG+VVNIPLSENVEGVVQPYHAYSPSGT YGPAVFVNYGRDEDYRELAKMG
Sbjct: 121 STSLSVLLSNGTVVNIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMG 180

Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240
           VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLY E D FRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKE----- 300
           GWAAVDGAERLNLNDSEVLKRFPKIPSMPLS+ESAEIIL+SLDTASVPPEWR ++     
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRTRKPILGR 300

Query: 301 ------ANLRSAAVGPGGPMF--------------------------------------- 360
                     S ++    P+                                        
Sbjct: 301 RRWGLVVRFLSTSLTRRRPLLMRSDFCFTAVNYLSNYNCPSLTNLDHNCGDLSGKNSSTT 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 QEYLLLPCHLMIYYFVSWVPHNTSCVFLMLTLHKLQVGKVLLRLLQGQYTKNMGQHCRIS 420

Query: 421 -----------------INFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFG 480
                            +N+  +GERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFG
Sbjct: 421 GVLGVYNLSEIKIKNISVNWKQKGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFG 480

Query: 481 AVDPNSGTAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGT 540
           AVDPNSGTAALLDIARRF+LLR+LGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGT
Sbjct: 481 AVDPNSGTAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGT 540

Query: 541 KAVAYLNVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIE 600
           KAVAYLNVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIE
Sbjct: 541 KAVAYLNVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIE 600

Query: 601 RLGAVNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIW 660
           RLGAVNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIW
Sbjct: 601 RLGAVNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIW 660

Query: 661 GLLALRLSDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIEN 702
           GLLALRLSDDLILPFSY+SYANQLQAYKDTLNHLLDGSVSL +LS+SIEELK AAQEIEN
Sbjct: 661 GLLALRLSDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIEN 720

BLAST of MELO3C009584 vs. NCBI nr
Match: XP_023539721.1 (probable glutamate carboxypeptidase AMP1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1219.1 bits (3153), Expect = 0.0e+00
Identity = 599/701 (85.45%), Postives = 643/701 (91.73%), Query Frame = 0

Query: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60
           M  PPLKQL T+C+S+P+P+ T  F IIICVLGFY FH SSSSSFSVTSS RNSVR++Q 
Sbjct: 1   MAPPPLKQLTTLCSSKPSPVTTLLFFIIICVLGFYTFHSSSSSSFSVTSSLRNSVRYRQF 60

Query: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
           LLSS SNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLG ETHSI+YDALLSYPK
Sbjct: 61  LLSSASNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGFETHSIRYDALLSYPK 120

Query: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180
             SL+    NGSVV IPLSENVEGVV+PYHAYSPSGT YG AVFVNYGRDEDYR LA MG
Sbjct: 121 YASLTARLPNGSVVQIPLSENVEGVVKPYHAYSPSGTAYGVAVFVNYGRDEDYRALAAMG 180

Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240
           VTV GCIAVARKGEFPRGVVVAKAEANG KGVLLY + D +RQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVAGCIAVARKGEFPRGVVVAKAEANGVKGVLLYADSDGYRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300
           GWAA+DGAERLNLNDSEVL++FPKIPSMPLS+E+AEIIL+SLDTASVPPEWRD    L +
Sbjct: 241 GWAAIDGAERLNLNDSEVLRKFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTIKLGT 300

Query: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
           AAVGPGGP ++NFTYQGERK+ATI NV+AVIKGLEEPDRFV+MGNHRDAWSFGAVDPNSG
Sbjct: 301 AAVGPGGPTYLNFTYQGERKIATIHNVLAVIKGLEEPDRFVIMGNHRDAWSFGAVDPNSG 360

Query: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
           TAALLDIARRFALLRKLGW+PRRT+LLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN
Sbjct: 361 TAALLDIARRFALLRKLGWSPRRTLLLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420

Query: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
           VDCAVQGPGFF+GATPQLDDLLH+VTAQV+DPDVKGATV+DTWTA NGI NIERLGA+NS
Sbjct: 421 VDCAVQGPGFFSGATPQLDDLLHEVTAQVEDPDVKGATVYDTWTATNGIANIERLGALNS 480

Query: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
           DFAAFVQHAGVPS+DVYYGRDFPVYHTAFDTYDWMA+YGDPLFHRH  VGSIWGLLALRL
Sbjct: 481 DFAAFVQHAGVPSIDVYYGRDFPVYHTAFDTYDWMASYGDPLFHRHAAVGSIWGLLALRL 540

Query: 541 SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE 600
           +DDLILPFSYVSYANQLQAY+D LN +LDGSVSL+ LSTSI E KSAA+EIENEAKRLRE
Sbjct: 541 ADDLILPFSYVSYANQLQAYRDILNDILDGSVSLQPLSTSIHEFKSAAEEIENEAKRLRE 600

Query: 601 QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA 660
           QET + VALFQ RALNDRLMLAERGFLDVDGLRGR WFKHLVYGP SDYESAL YFPGIA
Sbjct: 601 QETFTAVALFQTRALNDRLMLAERGFLDVDGLRGRTWFKHLVYGPSSDYESALAYFPGIA 660

Query: 661 DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 702
           DA+SES +MN+   + +IQHEIWRV RAI RAAAALKGELS
Sbjct: 661 DAISESTKMNQSEGQGVIQHEIWRVVRAITRAAAALKGELS 701

BLAST of MELO3C009584 vs. ExPASy Swiss-Prot
Match: Q9M1S8 (Probable glutamate carboxypeptidase AMP1 OS=Arabidopsis thaliana OX=3702 GN=AMP1 PE=1 SV=3)

HSP 1 Score: 781.9 bits (2018), Expect = 5.9e-225
Identity = 402/710 (56.62%), Postives = 516/710 (72.68%), Query Frame = 0

Query: 3   QPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSS--FSVTSSPRNSVRFQQL 62
           +P +  ++ I   +P PL +F FVI++ V  FY  H   + +     + +  N++R ++L
Sbjct: 8   RPTVTGISIIPFRQPPPLCSFLFVIVLFVATFYTLHHPDAVTPPLLFSRNAYNALRLRRL 67

Query: 63  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 122
            LSS SN T++SYLR LT HPHLAGT+PS +T+ YV +HF+ LGLETH  +Y+ALLSYP 
Sbjct: 68  FLSSASNATISSYLRELTRHPHLAGTKPSLDTLHYVFNHFQSLGLETHVAEYEALLSYPT 127

Query: 123 STSLSVLFSNGSVVNIPLSENVEG---VVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELA 182
             S++  FSN + +   L++ V G   VV+PYHAYSPSG+  G  VFVN+G + DY  L 
Sbjct: 128 HISVTASFSNTTTLEFDLND-VPGDSPVVRPYHAYSPSGSAQGNVVFVNHGEERDYHALE 187

Query: 183 KMGVTVVGCIAVARKGE-FPRGVVVAKAEANGAKGVLLYTEDDRFR-QGFERGTVMRGIG 242
            +GV+V GC+ +ARKGE   RG +V  AEA GA GVL+Y E+D     G ERGTVMRGIG
Sbjct: 188 SIGVSVKGCVVLARKGENLGRGAIVKIAEAKGALGVLIYAENDGGGFGGIERGTVMRGIG 247

Query: 243 DPLSPGWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKE 302
           DP+SPGW  V G E+L+L+D  V +RFPKIPS+PLS  +AEIIL SL  A  P EWR+  
Sbjct: 248 DPVSPGWPGVVGGEKLSLDDELVTRRFPKIPSLPLSLRNAEIILASLGGARAPLEWRN-- 307

Query: 303 ANLRSAAVGPG-----GPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAW 362
               S  VGPG     G M IN T+QGE K+  I NV+  I+G EE DR+V++GNHRDAW
Sbjct: 308 ----SGRVGPGQRVGPGRMVINMTFQGEMKMKKINNVVVTIRGSEEADRYVILGNHRDAW 367

Query: 363 SFGAVDPNSGTAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVN 422
           ++GAVDPNSGT+ALLDI+RRFALL K GW PRRTILLCSWDAEEFGMIGSTEW+E+N++N
Sbjct: 368 TYGAVDPNSGTSALLDISRRFALLLKSGWRPRRTILLCSWDAEEFGMIGSTEWIEENVLN 427

Query: 423 LGTKAVAYLNVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIG 482
           LG  AVAYLNVDCAVQG GFFAGATPQLD LL DV   VQDPD  G TV +T+ ++N I 
Sbjct: 428 LGASAVAYLNVDCAVQGSGFFAGATPQLDGLLVDVLKLVQDPDAVGLTVEETFKSQNNI- 487

Query: 483 NIERLGAVNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVG 542
            I+RL  V+SDF+ F+ HAG+PS+D+YYG D+PVYHTAFD+YDWM +  DPLFHRHV + 
Sbjct: 488 -IQRLSRVDSDFSGFLHHAGIPSIDMYYGADYPVYHTAFDSYDWMIHNADPLFHRHVAMA 547

Query: 543 SIWGLLALRLSDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQE 602
            IWGLL + L+D+ ++PF Y+SYA+QLQA++D L+ LL+G VS+  LS +I+E    A+E
Sbjct: 548 GIWGLLGILLADEPLIPFDYISYADQLQAHRDKLSKLLEGKVSVNPLSMAIQEFSLVAKE 607

Query: 603 IENEAKRLREQETSSD--VALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSD 662
             +EAK+L+ +  S +   A  ++R LNDRLML ERGFLD +G++G+ WFKHLVYGP ++
Sbjct: 608 AADEAKKLKGKSYSKNDVAAAAKRRELNDRLMLVERGFLDAEGIKGKEWFKHLVYGPAAE 667

Query: 663 YESALVYFPGIADAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKG 699
            ES L +FPGIADA++    MN    E +I+HEIWRVARAI RA+ ALKG
Sbjct: 668 PESKLGFFPGIADAIA----MN--ASEGIIEHEIWRVARAIQRASKALKG 702

BLAST of MELO3C009584 vs. ExPASy Swiss-Prot
Match: A0A1D6L709 (Probable glutamate carboxypeptidase VP8 OS=Zea mays OX=4577 GN=VP8 PE=2 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 2.0e-196
Identity = 351/669 (52.47%), Postives = 464/669 (69.36%), Query Frame = 0

Query: 38  HFSSSSSFSVTSSPRNSVRFQQLLLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVE 97
           H +++++    + P  S  F  L  S G+N ++A+ LR+LT  PHLAGT  S+    +V 
Sbjct: 39  HVAAAAASDRLTDPSRSRLF--LSQSPGANASIAADLRALTAGPHLAGTPASAGAAAHVL 98

Query: 98  SHFRDLGLETHSIQYDALLSYPKSTSLSVLFSNGSVV-NIPLSENVE---GVVQPYHAYS 157
           +  R  GL+T + +Y+ LLSYP   SL++L  +GS++  + L E  +    VV PYHAY+
Sbjct: 99  ARLRAAGLQTLTREYEPLLSYPGHASLALLRPDGSLLARLSLEEPADEGRRVVPPYHAYA 158

Query: 158 PSGTVYGPAVFVNYGRDEDYRELAKMGVTVVGCIAVARKGEFPRGVVVAKAEANGAKGVL 217
           PSG     AVFVN GR+EDY  L ++GV V G +AVAR+G   RG VVA+A   GA  VL
Sbjct: 159 PSGGAVAEAVFVNLGREEDYVVLERLGVGVRGRVAVARRGGGYRGGVVARAADKGAVAVL 218

Query: 218 LYTEDDRFRQGFERGTV-MRGIGDPLSPGWAAVDGAERLNLNDSEVLKRFPKIPSMPLSS 277
           +    D    G ERG V + G GDPL+PGWAA  GAERL  +D  V +RFP IPSMP+S+
Sbjct: 219 IAGNAD---GGVERGVVLLGGPGDPLTPGWAATSGAERLKFDDKAVKQRFPSIPSMPVSA 278

Query: 278 ESAEIILNSLDTASVPPEWRDKEANLRSAAVGPGGPMFINFTYQGERKVATIRNVIAVIK 337
           ++A  I+ SL   ++P EW+D    + +  +GP GP  +NFTYQ +RK   IR++  +IK
Sbjct: 279 KTAAAIIRSLGGPAIPAEWKD-GLGVDTGGLGP-GPTLVNFTYQEDRKFYKIRDIFGIIK 338

Query: 338 GLEEPDRFVLMGNHRDAWSFGAVDPNSGTAALLDIARRFALLRKLGWNPRRTILLCSWDA 397
           G EEPDR+V++GNHRDAW++GAVDPNSGTAALLDIARR  ++ + GW PRR+I+LCSWD 
Sbjct: 339 GQEEPDRYVILGNHRDAWTYGAVDPNSGTAALLDIARRLGIMLQSGWKPRRSIILCSWDG 398

Query: 398 EEFGMIGSTEWVEQNIVNLGTKAVAYLNVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDP 457
           EEFGMIGSTEWVE N+ +L +KAVAYLNVDCAVQG GFFAG+TPQLD LL D+T QV+DP
Sbjct: 399 EEFGMIGSTEWVEDNLEDLHSKAVAYLNVDCAVQGVGFFAGSTPQLDKLLVDITRQVRDP 458

Query: 458 DVKGATVHDTWTAENGIGNIERLGAVNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTY 517
           DV G  VHDTW   +G   IERL   +SDFA F+ HAG+PSVD+YYG +FP YHTA DTY
Sbjct: 459 DVTGKMVHDTWNEMSGGIKIERLARTDSDFAPFLHHAGIPSVDLYYGEEFPGYHTALDTY 518

Query: 518 DWMANYGDPLFHRHVTVGSIWGLLALRLSDDLILPFSYVSYANQLQAYKDTLNHLLDGSV 577
           +WM  +GDP F RH+ +  IWGLLALRL++D +LPF Y +Y +QLQ +  TL+ L     
Sbjct: 519 NWMEKHGDPFFLRHLAITEIWGLLALRLANDPVLPFDYQAYTSQLQEHIKTLSALTSNGH 578

Query: 578 SLRTLSTSIEELKSAAQEIENEAKRLREQETSSDVALFQKRALNDRLMLAERGFLDVDGL 637
           ++  ++  + +L  AA E+  E K+L++ +   + A  ++R LNDRL+LAER FL  +GL
Sbjct: 579 AVNLMNGCVNDLSGAAMEVLKEMKKLQQMDLYDEHARMRRRLLNDRLLLAERSFLQPEGL 638

Query: 638 RGRPWFKHLVYGPPSDYESALVYFPGIADAVSESKEMNKMGLEELIQHEIWRVARAIGRA 697
           +GR WFKHL+Y PP DYES L +FPGIADA+S S  ++    E  +QHE+W+V RAI RA
Sbjct: 639 QGRGWFKHLLYSPPEDYESKLSFFPGIADAISRSANLSDKEQEVAMQHEVWKVCRAIQRA 698

Query: 698 AAALKGELS 702
           A+ L+GE S
Sbjct: 699 ASVLRGEFS 700

BLAST of MELO3C009584 vs. ExPASy Swiss-Prot
Match: Q852M4 (Probable glutamate carboxypeptidase PLA3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA3 PE=1 SV=2)

HSP 1 Score: 684.5 bits (1765), Expect = 1.3e-195
Identity = 346/648 (53.40%), Postives = 460/648 (70.99%), Query Frame = 0

Query: 60  LLLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYP 119
           L LS G+N T+A+ LR+LT  PHLAGT  ++     V S FR  GL T + +Y  LLSYP
Sbjct: 92  LALSEGANATIAADLRALTAGPHLAGTGAAAGAAARVLSGFRAAGLRTLTREYTPLLSYP 151

Query: 120 KSTSLSVLFSNGSVV-NIPLSENVE---GVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRE 179
              SL++L ++ +++ ++ L E  +    +V+PYHAY+PSG     AVFVN GR+EDY  
Sbjct: 152 GHASLALLRADRTLLAHLSLDEPADVGRRLVRPYHAYAPSGGAVAEAVFVNLGREEDYLT 211

Query: 180 LAKMGVTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTV-MRGI 239
           L ++GV+V G +AVA +G   RG VV +A    A  VL+    D    G ERGTV + G 
Sbjct: 212 LERLGVSVRGRVAVAIRGGGYRGGVVRRAAERSAAAVLIAGHAD---GGVERGTVILGGP 271

Query: 240 GDPLSPGWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDK 299
           GDPL+PGWAA  GAERL+ +  +V +RFP IPSMP+S ++A  I+ +L   ++P +W+  
Sbjct: 272 GDPLTPGWAATAGAERLDFDHEDVKRRFPAIPSMPVSGKTASAIIRTLGGPALPADWQTG 331

Query: 300 EA-NLRSAAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFG 359
               +    VGP GP  +NFTYQ +RK+  I+++ A+IKG EEPDR+V++GNHRDAW++G
Sbjct: 332 VGLPVDVGGVGP-GPTLVNFTYQEDRKMGMIQDIFAIIKGYEEPDRYVILGNHRDAWTYG 391

Query: 360 AVDPNSGTAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGT 419
           AVDPNSGT+ALLDIARR  ++ + GW PRRTI+LCSWDAEEFGMIGSTEWVE+N+ +L +
Sbjct: 392 AVDPNSGTSALLDIARRLGIMLQSGWTPRRTIILCSWDAEEFGMIGSTEWVEENLEDLQS 451

Query: 420 KAVAYLNVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIE 479
           KAVAYLNVDCAVQG G FAG+TPQLD+LL DVT QV+DPDV+G TVHDTW    G  NIE
Sbjct: 452 KAVAYLNVDCAVQGIGLFAGSTPQLDNLLVDVTRQVKDPDVEGKTVHDTWNKMTGGINIE 511

Query: 480 RLGAVNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIW 539
           RL   +SDFA F+ HAG+P +D+YYG++FP YHTA D+Y WM  +GDPLF RHV +  IW
Sbjct: 512 RLARTDSDFAPFLHHAGIPCMDLYYGKEFPGYHTALDSYHWMEKHGDPLFLRHVAIVEIW 571

Query: 540 GLLALRLSDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIEN 599
           GLLALRL+DD +LPF Y +YA+QLQ + +  + +++ S  +  L+ SIE+L  A  E   
Sbjct: 572 GLLALRLADDPVLPFDYQTYASQLQEHANAFSSMMENSKWVHLLNRSIEDLSDAGLEFLK 631

Query: 600 EAKRLREQETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESAL 659
           EAK+L++Q  S   +L ++R LNDRL+LAER FL  DGL+GR WFKHL+Y PP DYES L
Sbjct: 632 EAKKLQDQNISDGYSLMRRRLLNDRLLLAERSFLQADGLQGRGWFKHLMYSPPEDYESKL 691

Query: 660 VYFPGIADAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 702
            +FPG+ADA+S S   +    +  ++HE+ +++RAI RAA  L+GE S
Sbjct: 692 SFFPGVADAISRSSNRSAKEQQAAVRHEVRKISRAIQRAADVLRGEFS 735

BLAST of MELO3C009584 vs. ExPASy Swiss-Prot
Match: Q7Y228 (Probable glutamate carboxypeptidase LAMP1 OS=Arabidopsis thaliana OX=3702 GN=LAMP1 PE=2 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 4.1e-162
Identity = 316/686 (46.06%), Postives = 429/686 (62.54%), Query Frame = 0

Query: 41  SSSSFSVTSSPRNSVRFQQLLLSS--GSNYTVASYLRSLTLHPHLAGTEPSSETVRYVES 100
           S S FS+ SSP  S  + +L +S+    N +VA  L +LT  PH+AGT  ++E   YV S
Sbjct: 15  SYSFFSLFSSPPKS-HYHELFISTSFSDNASVALNLHTLTRRPHVAGTVANAEAAEYVRS 74

Query: 101 HFRDLGLETHSIQYDALLSYPKSTSLSVLFSNGSVVNI-------PLSEN--VEGVVQPY 160
            F    L++H + Y   L+YP   SL VL    S   I        L +N     V+  +
Sbjct: 75  VFTSSALKSHVVAYQVSLTYPVHRSL-VLTPTDSAKPITFLLEQEKLGDNPYANEVMPTF 134

Query: 161 HAYSPSGTVYGPAVFVNYGRDEDYRELAK-MGVTVVGCIAVARKGEFPRGVVVAKAEANG 220
           H Y+ SG V GP V+ NYGR ED+  L K MGV V G + +AR G+  RG +V  A   G
Sbjct: 135 HGYAKSGNVSGPVVYANYGRVEDFVRLKKDMGVNVSGAVVIARYGQIYRGDIVKNAYEAG 194

Query: 221 AKGVLLYTE------DDRF-------RQGFERGTVMRGIGDPLSPGWAAVDGAERLNLND 280
           A GV++YT+      D+ F         G + GTV  G+GDP +PGWA+VDG ERL+   
Sbjct: 195 AVGVVIYTDKRDYGGDEWFPASKWMPPSGVQVGTVYNGLGDPTTPGWASVDGCERLSDEA 254

Query: 281 SEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRSAAVGPGGPMFINFTY 340
            E+    P IPS+P+S+  AE+IL      +V  +  D +       VGP GP  +N +Y
Sbjct: 255 VELSGDVPLIPSLPVSAADAEVILK-----TVVGDVSDGDV----YPVGP-GPGVLNLSY 314

Query: 341 QGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAALLDIARRFALLR 400
            GE  +A I NVI VI+G EEPDR+V++GNHRDAW+FGAVDPNSGTA L++IA+R   L+
Sbjct: 315 IGETVIAKIENVIGVIEGEEEPDRYVILGNHRDAWTFGAVDPNSGTAVLMEIAQRLDKLQ 374

Query: 401 KLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLNVDCAVQGPGFFAGAT 460
           K GW PRRTI+LC+WDAEE+G+IGSTEWVE+N   L ++AVAYLNVDCAV GPGF A AT
Sbjct: 375 KRGWKPRRTIILCNWDAEEYGLIGSTEWVEENREMLSSRAVAYLNVDCAVSGPGFHASAT 434

Query: 461 PQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNSDFAAFVQHAGVPSVD 520
           PQLD+L+     +V+DPD    T++++W   +    I RLG   SD+A+FVQH GVP VD
Sbjct: 435 PQLDELIKVAAQEVRDPDNATQTIYESWIGSSDSVVIRRLGGGGSDYASFVQHVGVPGVD 494

Query: 521 VYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRLSDDLILPFSYVSYAN 580
           + +GR +PVYH+ +D + WM  +GDP+F RHV + S+ GL+ALRL+D+ I+PF+Y SYA 
Sbjct: 495 MSFGRGYPVYHSMYDDFTWMEKFGDPMFQRHVAMASVLGLVALRLADEEIIPFNYTSYAL 554

Query: 581 QL-QAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLREQETSSDVALFQKRA 640
           +L ++ +D  N  L  ++ + TL  SIE+L +AA+ I  E + ++           + R 
Sbjct: 555 ELKKSAEDLENEKLGHNIDVSTLIKSIEDLSTAAKHISLEKEAIK--------GALKVRE 614

Query: 641 LNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIADAVSESKEMNKMGL 700
           LNDRLM+AER   D DGL  RPW+KHL+YGP    +     FPG+ DA+  +K++N    
Sbjct: 615 LNDRLMMAERALTDRDGLSERPWYKHLIYGPSKYDDYGSKSFPGVDDAIDNAKKLNTKAS 674

BLAST of MELO3C009584 vs. ExPASy Swiss-Prot
Match: P70627 (Glutamate carboxypeptidase 2 OS=Rattus norvegicus OX=10116 GN=Folh1 PE=1 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.2e-100
Identity = 238/692 (34.39%), Postives = 367/692 (53.03%), Query Frame = 0

Query: 31  VLGFYAFHF--SSSSSFSVTSSPRNSVRFQQLLLSSGSNYTVASYLRSLTLHPHLAGTEP 90
           ++GF    F   S+ S S  S P     F Q L +      +  +L + T  PHLAGT+ 
Sbjct: 35  IIGFLFGWFIKPSNDSTSSVSYPGMKKAFLQELKAE----NIKKFLYNFTRTPHLAGTQH 94

Query: 91  SSETVRYVESHFRDLGLETHSI-QYDALLSYPKSTS---LSVLFSNGSVV--------NI 150
           + E  + + + +++ GL+   +  YD LLSYP  T    +S++  +G+ +        + 
Sbjct: 95  NFELAKQIHAQWKEFGLDLVELSDYDVLLSYPNKTHPNYISIINEDGNEIFKTSLAELSP 154

Query: 151 PLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAK-MGVTVVGCIAVARKGEF 210
           P  EN+  VV PY A+SP GT  G  V+VNY R ED+ +L + M +   G I +AR G+ 
Sbjct: 155 PGYENISDVVPPYSAFSPQGTPEGDLVYVNYARTEDFFKLERVMKINCSGKIVIARYGQV 214

Query: 211 PRGVVVAKAEANGAKGVLLYTED-DRF--------------RQGFERGTV--MRGIGDPL 270
            RG  V  A+  GAKG++LY++  D F                G +RG V  + G GDPL
Sbjct: 215 FRGNKVKNAQLAGAKGIILYSDPADYFVPGVKSYPDGWNLPGGGVQRGNVLNLNGAGDPL 274

Query: 271 SPGWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANL 330
           +PG+ A + A R    ++  L   P IP  P+  + A+ +L  +   S PP+   K    
Sbjct: 275 TPGYPANEYAYRHEFTEAVGL---PSIPVHPIGYDDAQKLLEHMG-GSAPPDSSWKGGLK 334

Query: 331 RSAAVGPG-----GPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFG 390
               VGPG         +        KV  I NVI  +KG  EPDR+V++G HRDAW FG
Sbjct: 335 VPYNVGPGFAGNFSKQKVKLHIHSYNKVTRIYNVIGTLKGAVEPDRYVILGGHRDAWVFG 394

Query: 391 AVDPNSGTAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGT 450
            +DP SG A + +I R F  L+K GW PRRTIL  SWDAEEFG++GSTEW E++   L  
Sbjct: 395 GIDPQSGAAVVHEIVRTFGTLKKKGWRPRRTILFASWDAEEFGLLGSTEWAEEHSRLLQE 454

Query: 451 KAVAYLNVDCAVQGP-GFFAGATPQLDDLLHDVTAQVQDPD--VKGATVHDTWTAEN--- 510
           + VAY+N D +++G        TP +  L++++T ++  PD   +G +++D+W  ++   
Sbjct: 455 RGVAYINADSSIEGNYTLRVDCTPLMHSLVYNLTKELPSPDEGFEGKSLYDSWKEKSPST 514

Query: 511 ---GIGNIERLGAVNSDFAAFVQHAGVPSVDVYYGRD--------FPVYHTAFDTYDWMA 570
              G+  I +LG+ N DF  F Q  G+ S    Y ++        +P+YH+ ++TY+ + 
Sbjct: 515 EFIGMPRISKLGSGN-DFEVFFQRLGIASGRARYTKNWKNNKVSSYPLYHSVYETYELVE 574

Query: 571 NYGDPLFHRHVTVGSIWGLLALRLSDDLILPFSYVSYANQLQAYKDTL-NHLLDGSVSLR 630
            + DP F  H+TV  + G +   L++ ++LPF   SYA  L+ + +T+ N  ++    ++
Sbjct: 575 KFYDPTFKYHLTVAQVRGAMVFELANSIVLPFDCQSYAVALKKHAETIYNISMNHPQEMK 634

Query: 631 TLSTSIEELKSAAQEIENEA----KRLREQETSSDVALFQKRALNDRLMLAERGFLDVDG 664
               S + L SA     + A    +RL++ + S+ + L   R LND+LM  ER F+D  G
Sbjct: 635 AYMISFDSLFSAVNNFTDVASKFNQRLQDLDKSNPILL---RILNDQLMYLERAFIDPLG 694

BLAST of MELO3C009584 vs. ExPASy TrEMBL
Match: A0A5D3DNZ1 (Putative glutamate carboxypeptidase 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005020 PE=3 SV=1)

HSP 1 Score: 1403.3 bits (3631), Expect = 0.0e+00
Identity = 701/701 (100.00%), Postives = 701/701 (100.00%), Query Frame = 0

Query: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60
           MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL
Sbjct: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60

Query: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
           LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK
Sbjct: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120

Query: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180
           STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG
Sbjct: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180

Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240
           VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300
           GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300

Query: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
           AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG
Sbjct: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360

Query: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
           TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN
Sbjct: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420

Query: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
           VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS
Sbjct: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480

Query: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
           DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL
Sbjct: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540

Query: 541 SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE 600
           SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE
Sbjct: 541 SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE 600

Query: 601 QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA 660
           QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA
Sbjct: 601 QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA 660

Query: 661 DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 702
           DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS
Sbjct: 661 DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 701

BLAST of MELO3C009584 vs. ExPASy TrEMBL
Match: A0A1S3B7R6 (probable glutamate carboxypeptidase 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486721 PE=3 SV=1)

HSP 1 Score: 1403.3 bits (3631), Expect = 0.0e+00
Identity = 701/701 (100.00%), Postives = 701/701 (100.00%), Query Frame = 0

Query: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60
           MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL
Sbjct: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60

Query: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
           LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK
Sbjct: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120

Query: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180
           STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG
Sbjct: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180

Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240
           VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300
           GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300

Query: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
           AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG
Sbjct: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360

Query: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
           TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN
Sbjct: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420

Query: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
           VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS
Sbjct: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480

Query: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
           DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL
Sbjct: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540

Query: 541 SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE 600
           SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE
Sbjct: 541 SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE 600

Query: 601 QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA 660
           QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA
Sbjct: 601 QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA 660

Query: 661 DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 702
           DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS
Sbjct: 661 DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 701

BLAST of MELO3C009584 vs. ExPASy TrEMBL
Match: A0A0A0LEA0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G790960 PE=3 SV=1)

HSP 1 Score: 1340.9 bits (3469), Expect = 0.0e+00
Identity = 670/701 (95.58%), Postives = 683/701 (97.43%), Query Frame = 0

Query: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60
           MVQ PLKQLATICTSRPAPLPTFFFVIIICVLGFY FHFS+SSSFSVTSSPRNSVRFQQL
Sbjct: 1   MVQSPLKQLATICTSRPAPLPTFFFVIIICVLGFYTFHFSTSSSFSVTSSPRNSVRFQQL 60

Query: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
           LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK
Sbjct: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120

Query: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180
           STSLSVL SNG+VV IPLSENVEGVVQPYHAYSPSGT YGPAVFVNYGRDEDYRELAKMG
Sbjct: 121 STSLSVLLSNGTVVYIPLSENVEGVVQPYHAYSPSGTAYGPAVFVNYGRDEDYRELAKMG 180

Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240
           VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLY E D FRQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYVEGDGFRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300
           GWAAVDGAERLNLNDSEVLKRFPKIPSMPLS+ESAEIIL+SLDTASVPPEWRDK+ANL S
Sbjct: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSAESAEIILSSLDTASVPPEWRDKKANLGS 300

Query: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
           AAVGPGGP+FINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG
Sbjct: 301 AAVGPGGPIFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360

Query: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
           TAALLDIARRF+LLR+LGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN
Sbjct: 361 TAALLDIARRFSLLRRLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420

Query: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
           VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS
Sbjct: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480

Query: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
           DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL
Sbjct: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540

Query: 541 SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE 600
           SDDLILPFSY+SYANQLQAYKDTLNHLLDGSVSL +LS+SIEELK AAQEIENEAKRLRE
Sbjct: 541 SDDLILPFSYISYANQLQAYKDTLNHLLDGSVSLHSLSSSIEELKFAAQEIENEAKRLRE 600

Query: 601 QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA 660
           QE SSDVALFQKRALNDRLMLAERGFLDVDGLRG PWFKHLVYGP S+YESALVYFPGIA
Sbjct: 601 QEASSDVALFQKRALNDRLMLAERGFLDVDGLRGHPWFKHLVYGPLSNYESALVYFPGIA 660

Query: 661 DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 702
           DAVSESK +NK  LEELIQHEIWRVARAI RAAAALKGELS
Sbjct: 661 DAVSESKVINKRELEELIQHEIWRVARAIRRAAAALKGELS 701

BLAST of MELO3C009584 vs. ExPASy TrEMBL
Match: A0A6J1FMI1 (probable glutamate carboxypeptidase AMP1 OS=Cucurbita moschata OX=3662 GN=LOC111446753 PE=3 SV=1)

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 595/701 (84.88%), Postives = 643/701 (91.73%), Query Frame = 0

Query: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60
           M  PPLKQL T+C+S+P+P+ T  F IIICVLGFY FH SSSSSFS TSS RNSVR++Q 
Sbjct: 1   MAPPPLKQLTTLCSSKPSPVTTLLFFIIICVLGFYTFHSSSSSSFSATSSLRNSVRYRQF 60

Query: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
           LLSS SNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLG ETHSI+YDALLSYPK
Sbjct: 61  LLSSASNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGFETHSIRYDALLSYPK 120

Query: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180
             SL+    NGSVV IPLSENVEGVV+PYHAYSPSGT YG AVFVNYGRDEDYR LA MG
Sbjct: 121 YASLTARLPNGSVVQIPLSENVEGVVKPYHAYSPSGTAYGVAVFVNYGRDEDYRALAAMG 180

Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240
           VTV GCIAVARKGEFPRGVVVAKAEANG KGVLLY + D +RQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVAGCIAVARKGEFPRGVVVAKAEANGVKGVLLYADSDGYRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300
           GWAA+DGAERLNLNDSEVL++FPKIPSMPLS+E+AEIIL+SLDTASVPPEWRD    L +
Sbjct: 241 GWAAIDGAERLNLNDSEVLRKFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTIKLGT 300

Query: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
           AAVGPGGP ++NFTYQGERK+ATI NV+AVIKGLEEPDRFV+MGNHRDAWSFGAVDPNSG
Sbjct: 301 AAVGPGGPTYLNFTYQGERKIATIHNVLAVIKGLEEPDRFVIMGNHRDAWSFGAVDPNSG 360

Query: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
           TAALLDIARRFALLRKLGW+PRRT+LLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN
Sbjct: 361 TAALLDIARRFALLRKLGWSPRRTLLLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420

Query: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
           VDCAVQGPGFF+GATPQLDDLLH+VTAQV+DPDVKGATV+DTWTA NGIGNIERLGA+NS
Sbjct: 421 VDCAVQGPGFFSGATPQLDDLLHEVTAQVEDPDVKGATVYDTWTATNGIGNIERLGALNS 480

Query: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
           DFAAFVQHAGVPS+DVYYGRDFPVYHTAFDTYDWMA+YGDPLFHRH  VGSIWGLLALRL
Sbjct: 481 DFAAFVQHAGVPSIDVYYGRDFPVYHTAFDTYDWMASYGDPLFHRHAAVGSIWGLLALRL 540

Query: 541 SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE 600
           +DDLILPFSY SYANQLQAY+D LN +LDGSV+L++LSTSI E KSAA+EIENEAKRLRE
Sbjct: 541 ADDLILPFSYASYANQLQAYRDKLNDILDGSVALQSLSTSIHEFKSAAKEIENEAKRLRE 600

Query: 601 QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA 660
           QET + VALFQ RALNDRLMLAERGFLDV+GLRGR WFKHLVYGP SDYESAL YFPGIA
Sbjct: 601 QETFTAVALFQTRALNDRLMLAERGFLDVEGLRGRTWFKHLVYGPSSDYESALSYFPGIA 660

Query: 661 DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 702
           +A+SES +MN+   + +IQHEIWRV RAI RAAAALKGELS
Sbjct: 661 NAISESMKMNQSEGQGVIQHEIWRVVRAITRAAAALKGELS 701

BLAST of MELO3C009584 vs. ExPASy TrEMBL
Match: A0A6J1KUI3 (probable glutamate carboxypeptidase AMP1 OS=Cucurbita maxima OX=3661 GN=LOC111498787 PE=3 SV=1)

HSP 1 Score: 1205.7 bits (3118), Expect = 0.0e+00
Identity = 592/701 (84.45%), Postives = 638/701 (91.01%), Query Frame = 0

Query: 1   MVQPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSSFSVTSSPRNSVRFQQL 60
           M   PLKQL T+C+S+P+P+ T  F IIICVLGFY FH SSSSSF  TSS RNSVR++Q 
Sbjct: 1   MAPMPLKQLTTLCSSKPSPVTTLLFFIIICVLGFYTFHSSSSSSFFATSSLRNSVRYRQF 60

Query: 61  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 120
           LLSS SNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLG ETHSI+YDALLSYPK
Sbjct: 61  LLSSASNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGFETHSIRYDALLSYPK 120

Query: 121 STSLSVLFSNGSVVNIPLSENVEGVVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELAKMG 180
             SL+    NGSVV IPLSENVEGVV PYHAYSPSGT YG AVFVNYGRDEDYR LA MG
Sbjct: 121 YASLTARLPNGSVVQIPLSENVEGVVNPYHAYSPSGTAYGVAVFVNYGRDEDYRTLAAMG 180

Query: 181 VTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTEDDRFRQGFERGTVMRGIGDPLSP 240
           VTV GCIAVARKGEFPR VVVAKAEANG KGVLLYT+ D +RQGFERGTVMRGIGDPLSP
Sbjct: 181 VTVAGCIAVARKGEFPRSVVVAKAEANGVKGVLLYTDSDGYRQGFERGTVMRGIGDPLSP 240

Query: 241 GWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRS 300
           GWAA+DGAERLNLNDSEVL++FPKIPSMPLS+E+AEIIL+SLDTASVPPEWRD    L +
Sbjct: 241 GWAAIDGAERLNLNDSEVLRKFPKIPSMPLSAEAAEIILSSLDTASVPPEWRDTTVKLGT 300

Query: 301 AAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSG 360
           AAVGPGGP ++NFTYQ E+K+ATI NV+AVIKGLEEPDRFV+MGNHRDAWSFGAVDPNSG
Sbjct: 301 AAVGPGGPTYLNFTYQAEKKIATIHNVLAVIKGLEEPDRFVIMGNHRDAWSFGAVDPNSG 360

Query: 361 TAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420
           TAALLDIARRF+LLRKLGW+PRRT+LLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN
Sbjct: 361 TAALLDIARRFSLLRKLGWSPRRTLLLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLN 420

Query: 421 VDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNS 480
           VDCAVQGPGFF+GATPQLDDLLH+VTAQV+DPDVKGATV+DTWTA NGIGNIERLGA+NS
Sbjct: 421 VDCAVQGPGFFSGATPQLDDLLHEVTAQVEDPDVKGATVYDTWTATNGIGNIERLGALNS 480

Query: 481 DFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRL 540
           DFAAFVQHAGVPS+DVYYGRDFPVYHTAFDTYDWMA+YGDPLFHRH  VGSIWGLLALRL
Sbjct: 481 DFAAFVQHAGVPSIDVYYGRDFPVYHTAFDTYDWMASYGDPLFHRHAAVGSIWGLLALRL 540

Query: 541 SDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLRE 600
           +DDLILPFSYVSYANQLQAY+D LN +LDGSVSL++LSTSI E KSAA+EIE EAKRLRE
Sbjct: 541 ADDLILPFSYVSYANQLQAYRDILNDILDGSVSLQSLSTSIHEFKSAAEEIEKEAKRLRE 600

Query: 601 QETSSDVALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIA 660
           QET + VALFQ RALNDRLMLAERGFLDVDGLRG  WFKHLVYGP SDYESAL YFPGIA
Sbjct: 601 QETFTAVALFQTRALNDRLMLAERGFLDVDGLRGHTWFKHLVYGPSSDYESALAYFPGIA 660

Query: 661 DAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKGELS 702
           DA+SES +MN+   + +IQHEIWRV RAI RAAAALKGELS
Sbjct: 661 DAISESMKMNQSEGQGVIQHEIWRVVRAITRAAAALKGELS 701

BLAST of MELO3C009584 vs. TAIR 10
Match: AT3G54720.1 (Peptidase M28 family protein )

HSP 1 Score: 781.9 bits (2018), Expect = 4.2e-226
Identity = 402/710 (56.62%), Postives = 516/710 (72.68%), Query Frame = 0

Query: 3   QPPLKQLATICTSRPAPLPTFFFVIIICVLGFYAFHFSSSSS--FSVTSSPRNSVRFQQL 62
           +P +  ++ I   +P PL +F FVI++ V  FY  H   + +     + +  N++R ++L
Sbjct: 8   RPTVTGISIIPFRQPPPLCSFLFVIVLFVATFYTLHHPDAVTPPLLFSRNAYNALRLRRL 67

Query: 63  LLSSGSNYTVASYLRSLTLHPHLAGTEPSSETVRYVESHFRDLGLETHSIQYDALLSYPK 122
            LSS SN T++SYLR LT HPHLAGT+PS +T+ YV +HF+ LGLETH  +Y+ALLSYP 
Sbjct: 68  FLSSASNATISSYLRELTRHPHLAGTKPSLDTLHYVFNHFQSLGLETHVAEYEALLSYPT 127

Query: 123 STSLSVLFSNGSVVNIPLSENVEG---VVQPYHAYSPSGTVYGPAVFVNYGRDEDYRELA 182
             S++  FSN + +   L++ V G   VV+PYHAYSPSG+  G  VFVN+G + DY  L 
Sbjct: 128 HISVTASFSNTTTLEFDLND-VPGDSPVVRPYHAYSPSGSAQGNVVFVNHGEERDYHALE 187

Query: 183 KMGVTVVGCIAVARKGE-FPRGVVVAKAEANGAKGVLLYTEDDRFR-QGFERGTVMRGIG 242
            +GV+V GC+ +ARKGE   RG +V  AEA GA GVL+Y E+D     G ERGTVMRGIG
Sbjct: 188 SIGVSVKGCVVLARKGENLGRGAIVKIAEAKGALGVLIYAENDGGGFGGIERGTVMRGIG 247

Query: 243 DPLSPGWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKE 302
           DP+SPGW  V G E+L+L+D  V +RFPKIPS+PLS  +AEIIL SL  A  P EWR+  
Sbjct: 248 DPVSPGWPGVVGGEKLSLDDELVTRRFPKIPSLPLSLRNAEIILASLGGARAPLEWRN-- 307

Query: 303 ANLRSAAVGPG-----GPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAW 362
               S  VGPG     G M IN T+QGE K+  I NV+  I+G EE DR+V++GNHRDAW
Sbjct: 308 ----SGRVGPGQRVGPGRMVINMTFQGEMKMKKINNVVVTIRGSEEADRYVILGNHRDAW 367

Query: 363 SFGAVDPNSGTAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVN 422
           ++GAVDPNSGT+ALLDI+RRFALL K GW PRRTILLCSWDAEEFGMIGSTEW+E+N++N
Sbjct: 368 TYGAVDPNSGTSALLDISRRFALLLKSGWRPRRTILLCSWDAEEFGMIGSTEWIEENVLN 427

Query: 423 LGTKAVAYLNVDCAVQGPGFFAGATPQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIG 482
           LG  AVAYLNVDCAVQG GFFAGATPQLD LL DV   VQDPD  G TV +T+ ++N I 
Sbjct: 428 LGASAVAYLNVDCAVQGSGFFAGATPQLDGLLVDVLKLVQDPDAVGLTVEETFKSQNNI- 487

Query: 483 NIERLGAVNSDFAAFVQHAGVPSVDVYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVG 542
            I+RL  V+SDF+ F+ HAG+PS+D+YYG D+PVYHTAFD+YDWM +  DPLFHRHV + 
Sbjct: 488 -IQRLSRVDSDFSGFLHHAGIPSIDMYYGADYPVYHTAFDSYDWMIHNADPLFHRHVAMA 547

Query: 543 SIWGLLALRLSDDLILPFSYVSYANQLQAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQE 602
            IWGLL + L+D+ ++PF Y+SYA+QLQA++D L+ LL+G VS+  LS +I+E    A+E
Sbjct: 548 GIWGLLGILLADEPLIPFDYISYADQLQAHRDKLSKLLEGKVSVNPLSMAIQEFSLVAKE 607

Query: 603 IENEAKRLREQETSSD--VALFQKRALNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSD 662
             +EAK+L+ +  S +   A  ++R LNDRLML ERGFLD +G++G+ WFKHLVYGP ++
Sbjct: 608 AADEAKKLKGKSYSKNDVAAAAKRRELNDRLMLVERGFLDAEGIKGKEWFKHLVYGPAAE 667

Query: 663 YESALVYFPGIADAVSESKEMNKMGLEELIQHEIWRVARAIGRAAAALKG 699
            ES L +FPGIADA++    MN    E +I+HEIWRVARAI RA+ ALKG
Sbjct: 668 PESKLGFFPGIADAIA----MN--ASEGIIEHEIWRVARAIQRASKALKG 702

BLAST of MELO3C009584 vs. TAIR 10
Match: AT5G19740.1 (Peptidase M28 family protein )

HSP 1 Score: 573.2 bits (1476), Expect = 2.9e-163
Identity = 316/686 (46.06%), Postives = 429/686 (62.54%), Query Frame = 0

Query: 41  SSSSFSVTSSPRNSVRFQQLLLSS--GSNYTVASYLRSLTLHPHLAGTEPSSETVRYVES 100
           S S FS+ SSP  S  + +L +S+    N +VA  L +LT  PH+AGT  ++E   YV S
Sbjct: 15  SYSFFSLFSSPPKS-HYHELFISTSFSDNASVALNLHTLTRRPHVAGTVANAEAAEYVRS 74

Query: 101 HFRDLGLETHSIQYDALLSYPKSTSLSVLFSNGSVVNI-------PLSEN--VEGVVQPY 160
            F    L++H + Y   L+YP   SL VL    S   I        L +N     V+  +
Sbjct: 75  VFTSSALKSHVVAYQVSLTYPVHRSL-VLTPTDSAKPITFLLEQEKLGDNPYANEVMPTF 134

Query: 161 HAYSPSGTVYGPAVFVNYGRDEDYRELAK-MGVTVVGCIAVARKGEFPRGVVVAKAEANG 220
           H Y+ SG V GP V+ NYGR ED+  L K MGV V G + +AR G+  RG +V  A   G
Sbjct: 135 HGYAKSGNVSGPVVYANYGRVEDFVRLKKDMGVNVSGAVVIARYGQIYRGDIVKNAYEAG 194

Query: 221 AKGVLLYTE------DDRF-------RQGFERGTVMRGIGDPLSPGWAAVDGAERLNLND 280
           A GV++YT+      D+ F         G + GTV  G+GDP +PGWA+VDG ERL+   
Sbjct: 195 AVGVVIYTDKRDYGGDEWFPASKWMPPSGVQVGTVYNGLGDPTTPGWASVDGCERLSDEA 254

Query: 281 SEVLKRFPKIPSMPLSSESAEIILNSLDTASVPPEWRDKEANLRSAAVGPGGPMFINFTY 340
            E+    P IPS+P+S+  AE+IL      +V  +  D +       VGP GP  +N +Y
Sbjct: 255 VELSGDVPLIPSLPVSAADAEVILK-----TVVGDVSDGDV----YPVGP-GPGVLNLSY 314

Query: 341 QGERKVATIRNVIAVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAALLDIARRFALLR 400
            GE  +A I NVI VI+G EEPDR+V++GNHRDAW+FGAVDPNSGTA L++IA+R   L+
Sbjct: 315 IGETVIAKIENVIGVIEGEEEPDRYVILGNHRDAWTFGAVDPNSGTAVLMEIAQRLDKLQ 374

Query: 401 KLGWNPRRTILLCSWDAEEFGMIGSTEWVEQNIVNLGTKAVAYLNVDCAVQGPGFFAGAT 460
           K GW PRRTI+LC+WDAEE+G+IGSTEWVE+N   L ++AVAYLNVDCAV GPGF A AT
Sbjct: 375 KRGWKPRRTIILCNWDAEEYGLIGSTEWVEENREMLSSRAVAYLNVDCAVSGPGFHASAT 434

Query: 461 PQLDDLLHDVTAQVQDPDVKGATVHDTWTAENGIGNIERLGAVNSDFAAFVQHAGVPSVD 520
           PQLD+L+     +V+DPD    T++++W   +    I RLG   SD+A+FVQH GVP VD
Sbjct: 435 PQLDELIKVAAQEVRDPDNATQTIYESWIGSSDSVVIRRLGGGGSDYASFVQHVGVPGVD 494

Query: 521 VYYGRDFPVYHTAFDTYDWMANYGDPLFHRHVTVGSIWGLLALRLSDDLILPFSYVSYAN 580
           + +GR +PVYH+ +D + WM  +GDP+F RHV + S+ GL+ALRL+D+ I+PF+Y SYA 
Sbjct: 495 MSFGRGYPVYHSMYDDFTWMEKFGDPMFQRHVAMASVLGLVALRLADEEIIPFNYTSYAL 554

Query: 581 QL-QAYKDTLNHLLDGSVSLRTLSTSIEELKSAAQEIENEAKRLREQETSSDVALFQKRA 640
           +L ++ +D  N  L  ++ + TL  SIE+L +AA+ I  E + ++           + R 
Sbjct: 555 ELKKSAEDLENEKLGHNIDVSTLIKSIEDLSTAAKHISLEKEAIK--------GALKVRE 614

Query: 641 LNDRLMLAERGFLDVDGLRGRPWFKHLVYGPPSDYESALVYFPGIADAVSESKEMNKMGL 700
           LNDRLM+AER   D DGL  RPW+KHL+YGP    +     FPG+ DA+  +K++N    
Sbjct: 615 LNDRLMMAERALTDRDGLSERPWYKHLIYGPSKYDDYGSKSFPGVDDAIDNAKKLNTKAS 674

BLAST of MELO3C009584 vs. TAIR 10
Match: AT4G07670.1 (protease-associated (PA) domain-containing protein )

HSP 1 Score: 202.2 bits (513), Expect = 1.4e-51
Identity = 115/260 (44.23%), Postives = 154/260 (59.23%), Query Frame = 0

Query: 163 VFVNYGRDEDYRELAK-MGVTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTE---- 222
           V+ NYGR ED+  L K MGV V G + +AR G+  +  +V  A   GA GV++YT     
Sbjct: 11  VYANYGRVEDFVRLKKDMGVNVSGAVVIARYGQIYKVDIVNNAYEAGAVGVVIYTNKRDY 70

Query: 223 --DDRF-------RQGFERGTVMRGIGDPLSPGWAAVDGAERLNLNDSEVLKRFPKIPSM 282
             D+ F         GF+ GTV  G+GDP +PGWA+VDG ERL+    E+    P IPS+
Sbjct: 71  GGDEWFPASKWMPPSGFQVGTVYNGLGDPTTPGWASVDGCERLSDEAVELSGDVPLIPSL 130

Query: 283 PLSSESAEIILNSLDTASVPPEWRDKEANLRSAAVGPGGPMFINFTYQGERKVATIRNVI 342
           P+S+  AE+IL ++                    VGP GP  +N +Y     V  I+NVI
Sbjct: 131 PVSAADAEVILKTI-----------------VGDVGP-GPGILNLSY----IVTKIQNVI 190

Query: 343 AVIKGLEEPDRFVLMGNHRDAWSFGAVDPNSGTAALLD--------IARRFALLRKLGWN 401
            VI+G EEPDR+V++ NHRD W+F AVDPNSGTA L++        IA+R   L+K GW 
Sbjct: 191 GVIEGEEEPDRYVILRNHRDTWTFRAVDPNSGTAVLMEASKSYLQHIAQRLDKLQKRGWK 248

BLAST of MELO3C009584 vs. TAIR 10
Match: AT4G07670.2 (protease-associated (PA) domain-containing protein )

HSP 1 Score: 194.9 bits (494), Expect = 2.2e-49
Identity = 106/235 (45.11%), Postives = 143/235 (60.85%), Query Frame = 0

Query: 179 MGVTVVGCIAVARKGEFPRGVVVAKAEANGAKGVLLYTE------DDRF-------RQGF 238
           MGV V G + +AR G+  +  +V  A   GA GV++YT       D+ F         GF
Sbjct: 1   MGVNVSGAVVIARYGQIYKVDIVNNAYEAGAVGVVIYTNKRDYGGDEWFPASKWMPPSGF 60

Query: 239 ERGTVMRGIGDPLSPGWAAVDGAERLNLNDSEVLKRFPKIPSMPLSSESAEIILNSLDTA 298
           + GTV  G+GDP +PGWA+VDG ERL+    E+    P IPS+P+S+  AE+IL ++   
Sbjct: 61  QVGTVYNGLGDPTTPGWASVDGCERLSDEAVELSGDVPLIPSLPVSAADAEVILKTI--- 120

Query: 299 SVPPEWRDKEANLRSAAVGPGGPMFINFTYQGERKVATIRNVIAVIKGLEEPDRFVLMGN 358
                            VGP GP  +N +Y     V  I+NVI VI+G EEPDR+V++ N
Sbjct: 121 --------------VGDVGP-GPGILNLSY----IVTKIQNVIGVIEGEEEPDRYVILRN 180

Query: 359 HRDAWSFGAVDPNSGTAALLDIARRFALLRKLGWNPRRTILLCSWDAEEFGMIGS 401
           HRD W+F AVDPNSGTA L++IA+R   L+K GW PRRTI+LC+WDAEE+G++ S
Sbjct: 181 HRDTWTFRAVDPNSGTAVLMEIAQRLDKLQKRGWKPRRTIILCNWDAEEYGLVSS 213