MELO3C008894 (gene) Melon (DHL92) v4

Overview


Name	MELO3C008894
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	cysteine proteinase inhibitor A-like
Location	chr08: 23233813 .. 23234324 (+)
RNA-Seq Expression	MELO3C008894
Synteny	MELO3C008894

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTAAATCATCCTTAGAAAGCTTCTTCAATTCCAAGTACCAATAAAAAAGGCCATTAAAAAATGTCGATCATGTCCGCTTTTAAACCGATTGAGGATATCAATGATAATTTGGAAGTGCAAGATTTAGGAAACTTGACAGTGGATGACCATAACAAGAAAACAGGTGAAAATCTGAAGTTCGTGCGTGTGGTGAATGGTTTAGCTGCATCATTTTCTGACCCAATATCTCCTCTTGGAGGGGTACTATATGAGCTTGTGTTGGAGGCTATAACCACCAGCAAAATCAATTGGACATATAAGGCCAGGGTGTCGAAATCTAAGGCTATCACCCCACCCTTTCACATGTTGTATAGCTTCCAATCTTTTAAACCTGTTCTCGAGTACGAGCACCAACACTAATATGAGGCTCATCTCTCTGCTTCACAATATGCTTAAACAAGTATACTCTCGATGTCTTTAATTATCTATCAACGATATTAGCATTGCTATAAATAAAATACCAAATACTTATC

mRNA sequence

GTAAATCATCCTTAGAAAGCTTCTTCAATTCCAAGTACCAATAAAAAAGGCCATTAAAAAATGTCGATCATGTCCGCTTTTAAACCGATTGAGGATATCAATGATAATTTGGAAGTGCAAGATTTAGGAAACTTGACAGTGGATGACCATAACAAGAAAACAGGTGAAAATCTGAAGTTCGTGCGTGTGGTGAATGGTTTAGCTGCATCATTTTCTGACCCAATATCTCCTCTTGGAGGGGTACTATATGAGCTTGTGTTGGAGGCTATAACCACCAGCAAAATCAATTGGACATATAAGGCCAGGGTGTCGAAATCTAAGGCTATCACCCCACCCTTTCACATGTTGTATAGCTTCCAATCTTTTAAACCTGTTCTCGAGTACGAGCACCAACACTAATATGAGGCTCATCTCTCTGCTTCACAATATGCTTAAACAAGTATACTCTCGATGTCTTTAATTATCTATCAACGATATTAGCATTGCTATAAATAAAATACCAAATACTTATC

Coding sequence (CDS)

ATGTCGATCATGTCCGCTTTTAAACCGATTGAGGATATCAATGATAATTTGGAAGTGCAAGATTTAGGAAACTTGACAGTGGATGACCATAACAAGAAAACAGGTGAAAATCTGAAGTTCGTGCGTGTGGTGAATGGTTTAGCTGCATCATTTTCTGACCCAATATCTCCTCTTGGAGGGGTACTATATGAGCTTGTGTTGGAGGCTATAACCACCAGCAAAATCAATTGGACATATAAGGCCAGGGTGTCGAAATCTAAGGCTATCACCCCACCCTTTCACATGTTGTATAGCTTCCAATCTTTTAAACCTGTTCTCGAGTACGAGCACCAACACTAA

Protein sequence

MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGGVLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYEHQH

Homology

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BLAST of MELO3C008894 vs. NCBI nr
Match: XP_008442277.1 (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa] >TYK05422.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa])

HSP 1 Score: 226.1 bits (575), Expect = 1.5e-55
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 0


Query: 1   MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG 60
           MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG
Sbjct: 1   MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG 60

Query: 61  VLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYEHQH 113
           VLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYEHQH
Sbjct: 61  VLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYEHQH 112

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BLAST of MELO3C008894 vs. NCBI nr
Match: KAE8649716.1 (hypothetical protein Csa_012476 [Cucumis sativus])

HSP 1 Score: 152.9 bits (385), Expect = 1.6e-33
Identity = 77/112 (68.75%), Postives = 90/112 (80.36%), Query Frame = 0


Query: 1   MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG 60
           MS  S F+PI+DIND  +VQ++GNLTVDDHNKKTGENLKFVRVVNGL A F DP SPLGG
Sbjct: 1   MSFTSPFEPIKDINDP-QVQEIGNLTVDDHNKKTGENLKFVRVVNGLFAGFVDPFSPLGG 60

Query: 61  VLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYEHQH 113
            +YELVLEAI   KINWTYK +VS S  I  P  ++Y+FQSF+PVL+YE +H
Sbjct: 61  TVYELVLEAINAGKINWTYKVKVSASTTIR-PLGVVYNFQSFEPVLQYEEKH 110

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BLAST of MELO3C008894 vs. NCBI nr
Match: KAE8649717.1 (hypothetical protein Csa_012134, partial [Cucumis sativus])

HSP 1 Score: 91.7 bits (226), Expect = 4.4e-15
Identity = 45/57 (78.95%), Postives = 50/57 (87.72%), Query Frame = 0


Query: 12 DINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGGVLYELVLE 69
          DIND  +VQ++GNLTVDDHNKKTGENLKFVRVVNGL A F DP SPLGG +YELVL+
Sbjct: 1  DINDP-QVQEIGNLTVDDHNKKTGENLKFVRVVNGLFARFVDPFSPLGGTVYELVLD 56

[Close]

BLAST of MELO3C008894 vs. NCBI nr
Match: XP_031739698.1 (uncharacterized protein LOC116403239 [Cucumis sativus] >KAE8649718.1 hypothetical protein Csa_012480 [Cucumis sativus])

HSP 1 Score: 86.7 bits (213), Expect = 1.4e-13
Identity = 48/88 (54.55%), Postives = 62/88 (70.45%), Query Frame = 0


Query: 1  MSIMSAF---KPIEDINDNLEVQD-LGNLTVDDHNKKTGENLKFVRVVNGLAAS-FSDPI 60
          MS+  A+   KPIEDIND+L+VQ  +G L V+DHNKKTG+NL+ V VVNGL +  F  P 
Sbjct: 10 MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69

Query: 61 SPLGGVLYELVLEAITTSKINWTYKARV 84
          S   G+LY L++EA T   INWTY A++
Sbjct: 70 STHEGILYHLLVEAKTIEGINWTYVAKL 97

[Close]

BLAST of MELO3C008894 vs. NCBI nr
Match: TYK05428.1 (Proteinase inhibitor I25, cystatin [Cucumis melo var. makuwa])

HSP 1 Score: 86.3 bits (212), Expect = 1.9e-13
Identity = 48/88 (54.55%), Postives = 62/88 (70.45%), Query Frame = 0


Query: 1  MSIMSAF---KPIEDINDNLEVQD-LGNLTVDDHNKKTGENLKFVRVVNGLAAS-FSDPI 60
          MS+  A+   KPIE+IND+L+VQ  +G L V+DHNKKTGENL+FV VVNGL +  F  P 
Sbjct: 1  MSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGLRSEVFVQPG 60

Query: 61 SPLGGVLYELVLEAITTSKINWTYKARV 84
              G+LY L++EA T   INWTY A++
Sbjct: 61 CTHEGILYHLLVEAKTIEGINWTYVAKL 88

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BLAST of MELO3C008894 vs. ExPASy TrEMBL
Match: A0A5A7TJK0 (Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001140 PE=4 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 7.3e-56
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 0


Query: 1   MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG 60
           MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG
Sbjct: 1   MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG 60

Query: 61  VLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYEHQH 113
           VLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYEHQH
Sbjct: 61  VLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYEHQH 112

[Close]

BLAST of MELO3C008894 vs. ExPASy TrEMBL
Match: A0A1S3B5B9 (cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=4 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 7.3e-56
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 0


Query: 1   MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG 60
           MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG
Sbjct: 1   MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG 60

Query: 61  VLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYEHQH 113
           VLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYEHQH
Sbjct: 61  VLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYEHQH 112

[Close]

BLAST of MELO3C008894 vs. ExPASy TrEMBL
Match: A0A0A0L3W1 (Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE=4 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 3.3e-16
Identity = 56/113 (49.56%), Postives = 74/113 (65.49%), Query Frame = 0


Query: 1   MSIMSAF---KPIEDINDNLEVQD-LGNLTVDDHNKKTGENLKFVRVVNGLAAS-FSDPI 60
           MS+  A+   KPIEDIND+L+VQ  +G L V+DHNKKTG+NL+ V VVNGL +  F  P 
Sbjct: 10  MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69

Query: 61  SPLGGVLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEY 109
           S   G+LY L++EA T   INWTY   V+K   +     + Y F+SF+P+L Y
Sbjct: 70  STHEGILYHLLVEAKTIEGINWTY---VAKLLELYVGCRIRYEFKSFEPLLPY 119

[Close]

BLAST of MELO3C008894 vs. ExPASy TrEMBL
Match: A0A5D3C2L1 (Proteinase inhibitor I25, cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001230 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 9.0e-14
Identity = 48/88 (54.55%), Postives = 62/88 (70.45%), Query Frame = 0


Query: 1  MSIMSAF---KPIEDINDNLEVQD-LGNLTVDDHNKKTGENLKFVRVVNGLAAS-FSDPI 60
          MS+  A+   KPIE+IND+L+VQ  +G L V+DHNKKTGENL+FV VVNGL +  F  P 
Sbjct: 1  MSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGLRSEVFVQPG 60

Query: 61 SPLGGVLYELVLEAITTSKINWTYKARV 84
              G+LY L++EA T   INWTY A++
Sbjct: 61 CTHEGILYHLLVEAKTIEGINWTYVAKL 88

[Close]

BLAST of MELO3C008894 vs. ExPASy TrEMBL
Match: A0A0A0L191 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.9e-09
Identity = 47/102 (46.08%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 8   KPIEDINDNLEVQDLGNLTVDDHNK-KTGENLKFVRVVNGLAASFSDPISPLGGVLYELV 67
           KPI DIND   VQ +G L V+DH+    GE LKFVRVVNGL +           +LY LV
Sbjct: 11  KPIIDINDP-HVQIIGKLAVEDHDSHNPGEKLKFVRVVNGLKSDKYIGPGFTEVILYHLV 70

Query: 68  LEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEY 109
           LEA T  +INWTY  ++ K   +     + + F SF+PVL Y
Sbjct: 71  LEAKTNEEINWTYATKLEK---VYSGCLIRHVFLSFEPVLPY 108

The following BLAST results are available for this feature:

BLAST of MELO3C008894 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008442277.1	1.5e-55	100.00	PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cys...	[more]
KAE8649716.1	1.6e-33	68.75	hypothetical protein Csa_012476 [Cucumis sativus]	[more]
KAE8649717.1	4.4e-15	78.95	hypothetical protein Csa_012134, partial [Cucumis sativus]	[more]
XP_031739698.1	1.4e-13	54.55	uncharacterized protein LOC116403239 [Cucumis sativus] >KAE8649718.1 hypothetica...	[more]
TYK05428.1	1.9e-13	54.55	Proteinase inhibitor I25, cystatin [Cucumis melo var. makuwa]	[more]

BLAST of MELO3C008894 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MELO3C008894 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TJK0	7.3e-56	100.00	Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3B5B9	7.3e-56	100.00	cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=...	[more]
A0A0A0L3W1	3.3e-16	49.56	Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE...	[more]
A0A5D3C2L1	9.0e-14	54.55	Proteinase inhibitor I25, cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A0A0L191	3.9e-09	46.08	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE...	[more]

BLAST of MELO3C008894 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 1..112 e-value: 2.0E-15 score: 58.6
None	No IPR available	PANTHER	PTHR47116:SF5	PHLOEM FILAMENT PROTEIN	coord: 5..102
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 10..100
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 10..101 e-value: 3.2E-9 score: 37.0
IPR027214	Cystatin	PANTHER	PTHR47116	PHLOEM FILAMENT PROTEIN	coord: 5..102

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C008894.1	MELO3C008894.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity